LUBEL cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following LUBEL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LUBEL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13961 NM_001273232.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant K (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OFa13961 NM_001169425.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant D (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
OFa13959 NM_001273229.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant H (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa13960 NM_135228.4
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant C (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa13960 NM_001273227.1
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant F (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa13956 NM_001273230.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant I (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa13957 NM_001273228.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant G (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa13958 NM_001273231.2
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant J (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa16329 NM_164709.4
Latest version!
Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant E (LUBEL), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13961
    Clone ID Related Accession (Same CDS sequence) NM_001169425.2 , NM_001273232.2
    Accession Version NM_001169425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169425.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGG TACGCCACTG CACGATCTCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001162896.1
    CDS149..691
    Translation

    Target ORF information:

    RefSeq Version NM_001169425.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant D (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGG TACGCCACTG CACGATCTCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13961
    Clone ID Related Accession (Same CDS sequence) NM_001169425.2 , NM_001273232.2
    Accession Version NM_001273232.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273232.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGG TACGCCACTG CACGATCTCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260161.1
    CDS129..671
    Translation

    Target ORF information:

    RefSeq Version NM_001273232.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant K (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273232.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGG TACGCCACTG CACGATCTCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13959
    Clone ID Related Accession (Same CDS sequence) NM_001273229.2
    Accession Version NM_001273229.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273229.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGGTA GGATACAAAA GAGAATCTCA
    TTTTTCGAAG GCACAAAGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260158.1
    CDS129..2792
    Translation

    Target ORF information:

    RefSeq Version NM_001273229.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant H (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGGTA GGATACAAAA GAGAATCTCA
    TTTTTCGAAG GCACAAAGAC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13960
    Clone ID Related Accession (Same CDS sequence) NM_135228.4 , NM_001273227.1
    Accession Version NM_135228.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135228.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGGT
    AGGATACAAA AGAGAATCTC ATTTTTCGAA GGCACAAAGA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_609072.2
    CDS149..2653
    Translation

    Target ORF information:

    RefSeq Version NM_135228.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant C (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_135228.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGGT
    AGGATACAAA AGAGAATCTC ATTTTTCGAA GGCACAAAGA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13960
    Clone ID Related Accession (Same CDS sequence) NM_135228.4 , NM_001273227.1
    Accession Version NM_001273227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGGT
    AGGATACAAA AGAGAATCTC ATTTTTCGAA GGCACAAAGA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260156.1
    CDS263..2767
    Translation

    Target ORF information:

    RefSeq Version NM_001273227.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant F (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGGT
    AGGATACAAA AGAGAATCTC ATTTTTCGAA GGCACAAAGA CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13956
    Clone ID Related Accession (Same CDS sequence) NM_001273230.2
    Accession Version NM_001273230.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273230.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGAG
    TCCGTTGAAA ACATTTGGAA TACTCTGGAT GAGAGCATAC AGGCGCAGGC GGAGCAGGTA
    CTCAAGAAGG CTCAGAAGGT CTCCACGGCC TGTGGTGGGA CTCCTCCCCG GGAAATAGCA
    GCTGTGGAGA TGGGAACCTC CCCGCCTCCA CAAAGCATTT CCACACAGAC CTACGACGCT
    CTGCCCTTCA ATACAAAGCA AGAAGAGATC ATCCCCGTTG TTCCTGACCG CTTCACGACT
    CCAGAACCAA ACAAGATGGA GCGACGTCCG CACTATCGCA GCAATTCCCA GCTGCAACAG
    GAGAGTGAAC GTTATCGGAG TGCCAATGAC CTGAGGTATC ACGACGGCTT TGGATTGGAT
    CCATATAGTG CCGGTCTGGT CACCAAAAGA CCCAATTTCA TAAACGAGCT ACGCATGCTG
    CAGCTTCAAG TATCTTCACC TTTCGATATG CCGCATGAGA CATTCGGAGT CAAGCATGAG
    CCGGCCAGAG ATCCGGAAAC GGAAATGCAC ATCATACTGA AAGAACTGGA GCTGTACAAG
    TTTACGGTTG AGGAACTGGA AGCTGCCCTT AAGTACTGCT CCCCGGAGAC TCATCCGATT
    CAGTGGCTGC GCGAGAACTG GCACAAGCTG GTGCAGACGG TGCAGAGTTT GTCAACCAAG
    TACGGACAGG AGAGGGGTGA AAACACGATC GGAACTGTGT CCCAAAACGA AGCACGCGAG
    GCATTGCGCA ACTCGGGGGG AAATGTGTGG CAGGCGGTGG CTGACTGCAT CCAACAGAGG
    CAGCAGAAGT ACAGGAAACT GGCGGCCAAG GGAAACTTCC TGCGCGATGA CATTGTCAAC
    GCGCTGACTG CGCACCAAGG TAATGTGGAG CAGGCGCTGG TGGAGCTGAA TCGCACGCAA
    CTCAAGCCAT TTCTAATGCG CATCTGGGGC TCGCCGAATG GCGTTGAGAA TGAGAGTGGC
    GTGGCCATAG ACACCAAGTC GGATATCCAT GACTTCTTGA ACACACACGC CTTGGACTGC
    CTGCAGCCTC CGTTGGCTGG GCAAAGTCCC AGCCCCGCCC AAGCCAATCC TTTCGACCAA
    CCCCGCACCG ACGAGAGCCC CGTCAAATCC ACCTATGCCA CACCCAGTCC CTATCAGATG
    GAGGACAGCA CCCTGAAGAA CCTGGAGATA CTCATTGGCA ACATGGAACA GAACCAGGCC
    AAGCAGAACC AGGAGGTTCT ACGTTCCATC GAGACCATGC TGGACACATT CAAGGGAAAG
    CCGGAACTGG AGTACGAAAC GGATCCGGAG ATAATGCGTA TCCTAACAAA GAGCCCCATT
    AGCACAATGA AACCATCTGG ACCGGCAGAG GATAAGTCAA CCGATGATGT AAAGAACTTT
    GTATGGCAAC ACATTCAGGA AATTGTGCCC AACCTGGTGC AACAGGTTGA ACAGGAGCTC
    ATGGAGAAAC CGGAAGAGGT AGCTAAAATT GAGGCAGAAC AGCCAAAGGA GCCTGAACCT
    CTACTCGAAC CTCAGCCTGA GCCCACACCA AGTGTCGATC CTGCTGTCTA CATCATGGAG
    GAAGTCATCA AGCCCAATTT AAGGGAAGCC AGCATTCGTG AGGAAGTTCA ACCCAGTTTT
    ATCTATGCCA CTGAAATAGC CAACTTCAAG TTGGAATTTG ATCGTGGCAC TGAACGATGG
    CACGAGGCGG AATGGGAAAG CAGCGATCTT ACGGATGCAG AACGCATAGT ATACAAATGT
    TATATGGCTC CTAATGAGCA ACCCAAGGAG GATGTTGTAG ATGTAGCGGT TGAAAGTTCA
    GTCAATTCGC TTCCAACAGC AAAAGCACAA GAAGAATCTG CTCCAGAAGT ACCCATTGAA
    GCTCAAAAGA TTGAAAATAC AGAAGTTACA CAACCAGAGA CCGTTAATGA GGAACTTCGC
    CAGCAGGAAA AACTAGAAAC ACCATTAGTT ATTACAAGTG AGACAATTAG TGAAACAGTT
    TCGCAGACAG CCAATGAAAG TGATAAACAA AAATCAATTG AAAATAATTT GCAAATTAAG
    CAGAATGTGG CAGAAGTACA AGTTCAGTCG GATGACCAGC CAAGCACGAG TAGAGATGCC
    AACCGAAGAG CTAAGAGATC CCAGCAATCG AGAAAGGGTA GATCCCGCGA GCAAAGCCAA
    AAACCAACCA ATCGTACAAA GCTACCAAAC AATATCGATC AGAAAGTAAA TGAGTCAAAA
    ACAGCAGCTA AGGAAACAGA AGCAGTCAAA GATAAAGATC TGAGTGCTGC TGCAAGTAAT
    ATTCAGTCAG ATGTTACTGC ATCTGATCCT AAAACTAGCA CACCCCTTAA AATACTATCT
    GAAGGAACAA ATAGCAACAC TTTGGAAACT ATGGAAAACG TAACATCCAC AGATATAAAC
    GATAATGTTA CTATAGAGGT CATCTCAAAT CGATCCGAAG AAGTTCCAGC GATTCAGGAT
    TTGGGAAAAA CGAAGGACAT ATCTGAACCA ACTGCGAACC CAATCGAAGA AATTACATCC
    ATACAAAATA GTACGACTAT TTCAGAACAA TCTGAAGGGC CTCAAGAACC AGAAATACCT
    ATCGAAGTAT CTGAAACGAC AGAAGCACTT CAAGTCCCTC GAGAAGAAGC AAGCATTGAA
    ATTGTCAGCC CTCCAAACGA AGAACAAACC AAATCTCCAA CATCACAGGA AGTTAATATA
    CAAGACACAT CACACATCAT ATCTTTACCA ATTACTGATG TTACACCCAC TCCAGAAATC
    ATTAATATAG CGCCTAGTAC CTCATCAATA TCCAAAGAAC AAAAGCAAAG TCCGAAGCGT
    CTTTCAAAGA TTCCCGTAAG AACCCTGAGC AGCAGCAGTC TTCGCAGTGA GAGTCGGTCG
    AGCAATCGGA CTCCAACGGC CAATGATGAG ATCGAAAGGG AAGAGACCAC GTCACAAGGT
    GTACCCATAG GGGAAACAGT CAGTTCTCCA AAATCCGAAC AGTTGTCCGA CAACCAGGAG
    GTTAATCTTG TGAGCCAAGA AACACAGTCC AAAAAGGATA CAAATATTGT CGAAGAACCT
    GCCACCCAGC CTTTAGGCTT AGAGCTGGAA GAACATAGTC CGAATGCAAC TGCTGTAGCG
    GTCAGTCCCA CCGACTCGGA CGAAGTATTC GAAGATGCTC CTGAGTTCAG CGGTAGCGAT
    GGAACAAGAC CCCATGATGA AACCGCTTCC GATGCAGAGC TTTATAGCCT TGATAGCGAT
    GGGCAGCGGG CTGAAACCAA GTCCCCAGAG GATGAAGTCG TAATCTTGCT GGATGAGGAA
    TCCCAGATGG AATCTTCGAT AGCCCAGTCC GAAAGCAACG CAAGTCTTGA TTCCCACTCA
    AGTGAATCCG AGACGTCCAA AGTTGTTCTC AAGGAGTTTG TACCCTCTGG AGATCCGGCC
    AAACAGAACC TAAGCGAACT GGTTGAGGAC ACTCAACGTT TGATCAAGCA GATGCGCGAT
    GAGATCAGCA TGGATGAGTT CGAGTCTACC GATGAGGATG AATACTCCGA TGAATACTCG
    GATGAGTATG ACGAAGGGGA GGAGGAGGAG TGGTACGACA GCGAGGGTGA AGAGGAGGGA
    GACTTCGATG GGGAGGAGGG TAATACCTAT AACGAGCACG CATCCTACAT CGAGGAGGCC
    TCCACTGGTG ACGAAGGCAC CGAAATAGAG GACATAATGG AGGAGGATGA GGATCTGGCT
    GATGACGACG AGCCACTGCA ATCACAAATT CCACTAGACA TTGAACCAGT CATATCGCCT
    GCTCTATCAG TCACGCCCAC CAACCAAGAA ACTGATACGA TCGCACACAC TGAGGTTGTT
    TCCTCCACAG GAACGAGGCT GGAAACAGAG TTGCCGAACC CTGCAATGGA ATCCATCTTG
    CCTTCTCAAA GTGTTCAGGA GGACATCAAG GTGGAAGCGA TTCCTATTCA GTCCGCTCCC
    CCCATAGCTG ACTCGGAAAC TCGACCTGCA GAGCAACCAG TTGAGCTTGT GCTAGAAATC
    CCTTCAGAAG TTGAGCCTAC TCCCGTTGAA GAGCCCACTG CCCTGCCCAT AACACCTGCA
    CCACCAATTG TTGATTCCGA ATCGCGACCC GTGGAGCCGC CTGTTGAAAC AGTACTGGAA
    GAACCTAAAA AGGTAACCCC GAGCATGAAA GGCAAGACTG CAAACAGTGG AACTGCCTCC
    AAAGGTCCTT CCACATCAAG TTCGACTAAA ACAAACAAGT CCACAGTTAG TAAGATTCCC
    AAGCCCACCA ATGAACCCAC TAACAAATCG AATAGCACCC CCTTGAACAA GAAGGTTCCG
    CTGCGCTCCA AGTCCTTCTC GGCCCCTATG GGCATTTCAT CCGTGAAGCG AATCCAGGAG
    GTCTACCTCC AGAAGCAGAG TTCATCGATT GCAACCAGCC GAGTGCCGCT CAAGAGCAGT
    CCCGTCACCA AGAAGTCAAT CAACGATGCC ATCAGCAGGT TTAACTCCAA TCAGGCCGAT
    GGACCCAGCA CCAGTGGAGC AGCGGCTGCG GCAGCAGCGG CATTGTTGAA ACCTCGATCC
    CAACCCCGAA TCCCAAAGAA GAAATACCAC GAGACCTGCT TCTCTGACGA TGACTACGAG
    ACCTCGGCCA CGGAGGAGGA GCAGGAGGAA CCCAACTTGG CCGAGCCCCA AAAAGCAGAA
    CAGCTGAAGC GTAAGATGAG TATGCCCGTT TTTCGGGCAT ATCCTAGTGT TCAAGAGCCG
    GTAATCGAGG ATCCAGCGAT CCTTGCCCGC AAGTACGTCG ATCAGGAGCT GGTGACCAAT
    ATCGCAGAGG CCCAGATAGC CGCCACCTTG GTGAGCATGA AGTTCTCGGA AGACGTGGCT
    CTGTGGGCGG CCAGGGAGTG CTCCGATCTG GACCAGGCCA TTGCCATGCT CCAGCAGGAG
    TGCGAGCTCT GCATGAACAG CTATCCCATG AATCAGATGG TGTCCATGCT AAAGTGTCTG
    CACAAGTGCT GCAAGCAGTG TGCCAAGAGC TACTTTACAG TACAGATAAC CGATCGCAGT
    ATAAACGATT GCAGTTGCCC GTTTTGCAAG CTGCCCGAGT TGAGCAACGA GGCTCAGCAT
    GAGGATGAGC ACCTCGAGTA CTTCTCCAAC CTGGACATCT TCCTGAAGAG CATCCTGGAC
    AACGATGTGC ACGAACTGTT CCAGCGCAAG TTGCGCGATC GCTCGCTGCT GCAGGATCCC
    AACTTCAAGT GGTGCATCCA GTGCTCGTCC GGATTCTTCG CTCGGCCCAA GCAGAAGCGA
    TTGATTTGTC CGGACTGTGG ATCCGTTACC TGTGCCCAGT GCCGAAAGCC CTGGGAGCGC
    CAGCACGAGG GCAGTAGCTG CGAAGCTTAT CTGGAATGGA AGCGCGAAAA CGATCCCGAG
    CTGCAGGCCC AGGGTGTCCA GGAGCATTTG GCCCAAAACG GCATCGACTG TCCCAAGTGC
    AAGTTCCGAT ACTCGCTGGC CAGAGGCGGA TGCATGCATT TTACCTGTAC TCAGTGCAAA
    TTCGAGTTCT GCTACGGATG TGCCCGACCC TTCATGATGG GTGCCAAGTG CACTGTGTCC
    ACATACTGTG CCAAGCTCGG TCTCCATGCC CATCATCCGC GCAACTGCCT GTTCTACCTG
    AGGGATAAAA TCCCCCTTCA GTTGCAGTTC CTGCTCAAGG AGCAAAACGT CAAATTCGAC
    ACGGAACCCA TGCAGATCAA GGACGAGTCC AGCAGTTCGT CCAAGGCTCG TGCCCAGGCC
    CGCTGCCCGA TCCCGCTGCA AAAGGAGACG CCGCAAGGAC TAGTCGACAC AGTGTGCAAC
    ACGGAAGTTC CTGACAAGCA CGCTGGCATG TGTCGCACCC ACTACGTAGA GTATTTGGCT
    GGCAAGGTTG CCAAGGCTGG CATCGATCCA CTGCCCATCT TTGATCTGAC GGACTGCGTC
    CAGGAGCTGC GAAGGCGAGG CATCGCTCTG CCGGAGAGAG GACCCTGGGA CACCGATGAG
    ATCTACAAGA ACATGTGCTC CGAGGTAATC AAAAAGCATA TACCACTGAA ATCGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260159.1
    CDS129..8648
    Translation

    Target ORF information:

    RefSeq Version NM_001273230.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant I (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273230.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGAG
    TCCGTTGAAA ACATTTGGAA TACTCTGGAT GAGAGCATAC AGGCGCAGGC GGAGCAGGTA
    CTCAAGAAGG CTCAGAAGGT CTCCACGGCC TGTGGTGGGA CTCCTCCCCG GGAAATAGCA
    GCTGTGGAGA TGGGAACCTC CCCGCCTCCA CAAAGCATTT CCACACAGAC CTACGACGCT
    CTGCCCTTCA ATACAAAGCA AGAAGAGATC ATCCCCGTTG TTCCTGACCG CTTCACGACT
    CCAGAACCAA ACAAGATGGA GCGACGTCCG CACTATCGCA GCAATTCCCA GCTGCAACAG
    GAGAGTGAAC GTTATCGGAG TGCCAATGAC CTGAGGTATC ACGACGGCTT TGGATTGGAT
    CCATATAGTG CCGGTCTGGT CACCAAAAGA CCCAATTTCA TAAACGAGCT ACGCATGCTG
    CAGCTTCAAG TATCTTCACC TTTCGATATG CCGCATGAGA CATTCGGAGT CAAGCATGAG
    CCGGCCAGAG ATCCGGAAAC GGAAATGCAC ATCATACTGA AAGAACTGGA GCTGTACAAG
    TTTACGGTTG AGGAACTGGA AGCTGCCCTT AAGTACTGCT CCCCGGAGAC TCATCCGATT
    CAGTGGCTGC GCGAGAACTG GCACAAGCTG GTGCAGACGG TGCAGAGTTT GTCAACCAAG
    TACGGACAGG AGAGGGGTGA AAACACGATC GGAACTGTGT CCCAAAACGA AGCACGCGAG
    GCATTGCGCA ACTCGGGGGG AAATGTGTGG CAGGCGGTGG CTGACTGCAT CCAACAGAGG
    CAGCAGAAGT ACAGGAAACT GGCGGCCAAG GGAAACTTCC TGCGCGATGA CATTGTCAAC
    GCGCTGACTG CGCACCAAGG TAATGTGGAG CAGGCGCTGG TGGAGCTGAA TCGCACGCAA
    CTCAAGCCAT TTCTAATGCG CATCTGGGGC TCGCCGAATG GCGTTGAGAA TGAGAGTGGC
    GTGGCCATAG ACACCAAGTC GGATATCCAT GACTTCTTGA ACACACACGC CTTGGACTGC
    CTGCAGCCTC CGTTGGCTGG GCAAAGTCCC AGCCCCGCCC AAGCCAATCC TTTCGACCAA
    CCCCGCACCG ACGAGAGCCC CGTCAAATCC ACCTATGCCA CACCCAGTCC CTATCAGATG
    GAGGACAGCA CCCTGAAGAA CCTGGAGATA CTCATTGGCA ACATGGAACA GAACCAGGCC
    AAGCAGAACC AGGAGGTTCT ACGTTCCATC GAGACCATGC TGGACACATT CAAGGGAAAG
    CCGGAACTGG AGTACGAAAC GGATCCGGAG ATAATGCGTA TCCTAACAAA GAGCCCCATT
    AGCACAATGA AACCATCTGG ACCGGCAGAG GATAAGTCAA CCGATGATGT AAAGAACTTT
    GTATGGCAAC ACATTCAGGA AATTGTGCCC AACCTGGTGC AACAGGTTGA ACAGGAGCTC
    ATGGAGAAAC CGGAAGAGGT AGCTAAAATT GAGGCAGAAC AGCCAAAGGA GCCTGAACCT
    CTACTCGAAC CTCAGCCTGA GCCCACACCA AGTGTCGATC CTGCTGTCTA CATCATGGAG
    GAAGTCATCA AGCCCAATTT AAGGGAAGCC AGCATTCGTG AGGAAGTTCA ACCCAGTTTT
    ATCTATGCCA CTGAAATAGC CAACTTCAAG TTGGAATTTG ATCGTGGCAC TGAACGATGG
    CACGAGGCGG AATGGGAAAG CAGCGATCTT ACGGATGCAG AACGCATAGT ATACAAATGT
    TATATGGCTC CTAATGAGCA ACCCAAGGAG GATGTTGTAG ATGTAGCGGT TGAAAGTTCA
    GTCAATTCGC TTCCAACAGC AAAAGCACAA GAAGAATCTG CTCCAGAAGT ACCCATTGAA
    GCTCAAAAGA TTGAAAATAC AGAAGTTACA CAACCAGAGA CCGTTAATGA GGAACTTCGC
    CAGCAGGAAA AACTAGAAAC ACCATTAGTT ATTACAAGTG AGACAATTAG TGAAACAGTT
    TCGCAGACAG CCAATGAAAG TGATAAACAA AAATCAATTG AAAATAATTT GCAAATTAAG
    CAGAATGTGG CAGAAGTACA AGTTCAGTCG GATGACCAGC CAAGCACGAG TAGAGATGCC
    AACCGAAGAG CTAAGAGATC CCAGCAATCG AGAAAGGGTA GATCCCGCGA GCAAAGCCAA
    AAACCAACCA ATCGTACAAA GCTACCAAAC AATATCGATC AGAAAGTAAA TGAGTCAAAA
    ACAGCAGCTA AGGAAACAGA AGCAGTCAAA GATAAAGATC TGAGTGCTGC TGCAAGTAAT
    ATTCAGTCAG ATGTTACTGC ATCTGATCCT AAAACTAGCA CACCCCTTAA AATACTATCT
    GAAGGAACAA ATAGCAACAC TTTGGAAACT ATGGAAAACG TAACATCCAC AGATATAAAC
    GATAATGTTA CTATAGAGGT CATCTCAAAT CGATCCGAAG AAGTTCCAGC GATTCAGGAT
    TTGGGAAAAA CGAAGGACAT ATCTGAACCA ACTGCGAACC CAATCGAAGA AATTACATCC
    ATACAAAATA GTACGACTAT TTCAGAACAA TCTGAAGGGC CTCAAGAACC AGAAATACCT
    ATCGAAGTAT CTGAAACGAC AGAAGCACTT CAAGTCCCTC GAGAAGAAGC AAGCATTGAA
    ATTGTCAGCC CTCCAAACGA AGAACAAACC AAATCTCCAA CATCACAGGA AGTTAATATA
    CAAGACACAT CACACATCAT ATCTTTACCA ATTACTGATG TTACACCCAC TCCAGAAATC
    ATTAATATAG CGCCTAGTAC CTCATCAATA TCCAAAGAAC AAAAGCAAAG TCCGAAGCGT
    CTTTCAAAGA TTCCCGTAAG AACCCTGAGC AGCAGCAGTC TTCGCAGTGA GAGTCGGTCG
    AGCAATCGGA CTCCAACGGC CAATGATGAG ATCGAAAGGG AAGAGACCAC GTCACAAGGT
    GTACCCATAG GGGAAACAGT CAGTTCTCCA AAATCCGAAC AGTTGTCCGA CAACCAGGAG
    GTTAATCTTG TGAGCCAAGA AACACAGTCC AAAAAGGATA CAAATATTGT CGAAGAACCT
    GCCACCCAGC CTTTAGGCTT AGAGCTGGAA GAACATAGTC CGAATGCAAC TGCTGTAGCG
    GTCAGTCCCA CCGACTCGGA CGAAGTATTC GAAGATGCTC CTGAGTTCAG CGGTAGCGAT
    GGAACAAGAC CCCATGATGA AACCGCTTCC GATGCAGAGC TTTATAGCCT TGATAGCGAT
    GGGCAGCGGG CTGAAACCAA GTCCCCAGAG GATGAAGTCG TAATCTTGCT GGATGAGGAA
    TCCCAGATGG AATCTTCGAT AGCCCAGTCC GAAAGCAACG CAAGTCTTGA TTCCCACTCA
    AGTGAATCCG AGACGTCCAA AGTTGTTCTC AAGGAGTTTG TACCCTCTGG AGATCCGGCC
    AAACAGAACC TAAGCGAACT GGTTGAGGAC ACTCAACGTT TGATCAAGCA GATGCGCGAT
    GAGATCAGCA TGGATGAGTT CGAGTCTACC GATGAGGATG AATACTCCGA TGAATACTCG
    GATGAGTATG ACGAAGGGGA GGAGGAGGAG TGGTACGACA GCGAGGGTGA AGAGGAGGGA
    GACTTCGATG GGGAGGAGGG TAATACCTAT AACGAGCACG CATCCTACAT CGAGGAGGCC
    TCCACTGGTG ACGAAGGCAC CGAAATAGAG GACATAATGG AGGAGGATGA GGATCTGGCT
    GATGACGACG AGCCACTGCA ATCACAAATT CCACTAGACA TTGAACCAGT CATATCGCCT
    GCTCTATCAG TCACGCCCAC CAACCAAGAA ACTGATACGA TCGCACACAC TGAGGTTGTT
    TCCTCCACAG GAACGAGGCT GGAAACAGAG TTGCCGAACC CTGCAATGGA ATCCATCTTG
    CCTTCTCAAA GTGTTCAGGA GGACATCAAG GTGGAAGCGA TTCCTATTCA GTCCGCTCCC
    CCCATAGCTG ACTCGGAAAC TCGACCTGCA GAGCAACCAG TTGAGCTTGT GCTAGAAATC
    CCTTCAGAAG TTGAGCCTAC TCCCGTTGAA GAGCCCACTG CCCTGCCCAT AACACCTGCA
    CCACCAATTG TTGATTCCGA ATCGCGACCC GTGGAGCCGC CTGTTGAAAC AGTACTGGAA
    GAACCTAAAA AGGTAACCCC GAGCATGAAA GGCAAGACTG CAAACAGTGG AACTGCCTCC
    AAAGGTCCTT CCACATCAAG TTCGACTAAA ACAAACAAGT CCACAGTTAG TAAGATTCCC
    AAGCCCACCA ATGAACCCAC TAACAAATCG AATAGCACCC CCTTGAACAA GAAGGTTCCG
    CTGCGCTCCA AGTCCTTCTC GGCCCCTATG GGCATTTCAT CCGTGAAGCG AATCCAGGAG
    GTCTACCTCC AGAAGCAGAG TTCATCGATT GCAACCAGCC GAGTGCCGCT CAAGAGCAGT
    CCCGTCACCA AGAAGTCAAT CAACGATGCC ATCAGCAGGT TTAACTCCAA TCAGGCCGAT
    GGACCCAGCA CCAGTGGAGC AGCGGCTGCG GCAGCAGCGG CATTGTTGAA ACCTCGATCC
    CAACCCCGAA TCCCAAAGAA GAAATACCAC GAGACCTGCT TCTCTGACGA TGACTACGAG
    ACCTCGGCCA CGGAGGAGGA GCAGGAGGAA CCCAACTTGG CCGAGCCCCA AAAAGCAGAA
    CAGCTGAAGC GTAAGATGAG TATGCCCGTT TTTCGGGCAT ATCCTAGTGT TCAAGAGCCG
    GTAATCGAGG ATCCAGCGAT CCTTGCCCGC AAGTACGTCG ATCAGGAGCT GGTGACCAAT
    ATCGCAGAGG CCCAGATAGC CGCCACCTTG GTGAGCATGA AGTTCTCGGA AGACGTGGCT
    CTGTGGGCGG CCAGGGAGTG CTCCGATCTG GACCAGGCCA TTGCCATGCT CCAGCAGGAG
    TGCGAGCTCT GCATGAACAG CTATCCCATG AATCAGATGG TGTCCATGCT AAAGTGTCTG
    CACAAGTGCT GCAAGCAGTG TGCCAAGAGC TACTTTACAG TACAGATAAC CGATCGCAGT
    ATAAACGATT GCAGTTGCCC GTTTTGCAAG CTGCCCGAGT TGAGCAACGA GGCTCAGCAT
    GAGGATGAGC ACCTCGAGTA CTTCTCCAAC CTGGACATCT TCCTGAAGAG CATCCTGGAC
    AACGATGTGC ACGAACTGTT CCAGCGCAAG TTGCGCGATC GCTCGCTGCT GCAGGATCCC
    AACTTCAAGT GGTGCATCCA GTGCTCGTCC GGATTCTTCG CTCGGCCCAA GCAGAAGCGA
    TTGATTTGTC CGGACTGTGG ATCCGTTACC TGTGCCCAGT GCCGAAAGCC CTGGGAGCGC
    CAGCACGAGG GCAGTAGCTG CGAAGCTTAT CTGGAATGGA AGCGCGAAAA CGATCCCGAG
    CTGCAGGCCC AGGGTGTCCA GGAGCATTTG GCCCAAAACG GCATCGACTG TCCCAAGTGC
    AAGTTCCGAT ACTCGCTGGC CAGAGGCGGA TGCATGCATT TTACCTGTAC TCAGTGCAAA
    TTCGAGTTCT GCTACGGATG TGCCCGACCC TTCATGATGG GTGCCAAGTG CACTGTGTCC
    ACATACTGTG CCAAGCTCGG TCTCCATGCC CATCATCCGC GCAACTGCCT GTTCTACCTG
    AGGGATAAAA TCCCCCTTCA GTTGCAGTTC CTGCTCAAGG AGCAAAACGT CAAATTCGAC
    ACGGAACCCA TGCAGATCAA GGACGAGTCC AGCAGTTCGT CCAAGGCTCG TGCCCAGGCC
    CGCTGCCCGA TCCCGCTGCA AAAGGAGACG CCGCAAGGAC TAGTCGACAC AGTGTGCAAC
    ACGGAAGTTC CTGACAAGCA CGCTGGCATG TGTCGCACCC ACTACGTAGA GTATTTGGCT
    GGCAAGGTTG CCAAGGCTGG CATCGATCCA CTGCCCATCT TTGATCTGAC GGACTGCGTC
    CAGGAGCTGC GAAGGCGAGG CATCGCTCTG CCGGAGAGAG GACCCTGGGA CACCGATGAG
    ATCTACAAGA ACATGTGCTC CGAGGTAATC AAAAAGCATA TACCACTGAA ATCGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13957
    Clone ID Related Accession (Same CDS sequence) NM_001273228.2
    Accession Version NM_001273228.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273228.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGAGT CCGTTGAAAA CATTTGGAAT
    ACTCTGGATG AGAGCATACA GGCGCAGGCG GAGCAGGTAC TCAAGAAGGC TCAGAAGGTC
    TCCACGGCCT GTGGTGGGAC TCCTCCCCGG GAAATAGCAG CTGTGGAGAT GGGAACCTCC
    CCGCCTCCAC AAAGCATTTC CACACAGACC TACGACGCTC TGCCCTTCAA TACAAAGCAA
    GAAGAGATCA TCCCCGTTGT TCCTGACCGC TTCACGACTC CAGAACCAAA CAAGATGGAG
    CGACGTCCGC ACTATCGCAG CAATTCCCAG CTGCAACAGG AGAGTGAACG TTATCGGAGT
    GCCAATGACC TGAGGTATCA CGACGGCTTT GGATTGGATC CATATAGTGC CGGTCTGGTC
    ACCAAAAGAC CCAATTTCAT AAACGAGCTA CGCATGCTGC AGCTTCAAGT ATCTTCACCT
    TTCGATATGC CGCATGAGAC ATTCGGAGTC AAGCATGAGC CGGCCAGAGA TCCGGAAACG
    GAAATGCACA TCATACTGAA AGAACTGGAG CTGTACAAGT TTACGGTTGA GGAACTGGAA
    GCTGCCCTTA AGTACTGCTC CCCGGAGACT CATCCGATTC AGTGGCTGCG CGAGAACTGG
    CACAAGCTGG TGCAGACGGT GCAGAGTTTG TCAACCAAGT ACGGACAGGA GAGGGGTGAA
    AACACGATCG GAACTGTGTC CCAAAACGAA GCACGCGAGG CATTGCGCAA CTCGGGGGGA
    AATGTGTGGC AGGCGGTGGC TGACTGCATC CAACAGAGGC AGCAGAAGTA CAGGAAACTG
    GCGGCCAAGG GAAACTTCCT GCGCGATGAC ATTGTCAACG CGCTGACTGC GCACCAAGAT
    ATCCATGACT TCTTGAACAC ACACGCCTTG GACTGCCTGC AGCCTCCGTT GGCTGGGCAA
    AGTCCCAGCC CCGCCCAAGC CAATCCTTTC GACCAACCCC GCACCGACGA GAGCCCCGTC
    AAATCCACCT ATGCCACACC CAGTCCCTAT CAGATGGAGG ACAGCACCCT GAAGAACCTG
    GAGATACTCA TTGGCAACAT GGAACAGAAC CAGGCCAAGC AGAACCAGGA GGTTCTACGT
    TCCATCGAGA CCATGCTGGA CACATTCAAG GGAAAGCCGG AACTGGAGTA CGAAACGGAT
    CCGGAGATAA TGCGTATCCT AACAAAGAGC CCCATTAGCA CAATGAAACC ATCTGGACCG
    GCAGAGGATA AGTCAACCGA TGATGTAAAG AACTTTGTAT GGCAACACAT TCAGGAAATT
    GTGCCCAACC TGGTGCAACA GGTTGAACAG GAGCTCATGG AGAAACCGGA AGAGGTAGCT
    AAAATTGAGG CAGAACAGCC AAAGGAGCCT GAACCTCTAC TCGAACCTCA GCCTGAGCCC
    ACACCAAGTG TCGATCCTGC TGTCTACATC ATGGAGGAAG TCATCAAGCC CAATTTAAGG
    GAAGCCAGCA TTCGTGAGGA AGTTCAACCC AGTTTTATCT ATGCCACTGA AATAGCCAAC
    TTCAAGTTGG AATTTGATCG TGGCACTGAA CGATGGCACG AGGCGGAATG GGAAAGCAGC
    GATCTTACGG ATGCAGAACG CATAGTATAC AAATGTTATA TGGCTCCTAA TGAGCAACCC
    AAGGAGGATG TTGTAGATGT AGCGGTTGAA AGTTCAGTCA ATTCGCTTCC AACAGCAAAA
    GCACAAGAAG AATCTGCTCC AGAAGTACCC ATTGAAGCTC AAAAGATTGA AAATACAGAA
    GTTACACAAC CAGAGACCGT TAATGAGGAA CTTCGCCAGC AGGAAAAACT AGAAACACCA
    TTAGTTATTA CAAGTGAGAC AATTAGTGAA ACAGTTTCGC AGACAGCCAA TGAAAGTGAT
    AAACAAAAAT CAATTGAAAA TAATTTGCAA ATTAAGCAGA ATGTGGCAGA AGTACAAGTT
    CAGTCGGATG ACCAGCCAAG CACGAGTAGA GATGCCAACC GAAGAGCTAA GAGATCCCAG
    CAATCGAGAA AGGGTAGATC CCGCGAGCAA AGCCAAAAAC CAACCAATCG TACAAAGCTA
    CCAAACAATA TCGATCAGAA AGTAAATGAG TCAAAAACAG CAGCTAAGGA AACAGAAGCA
    GTCAAAGATA AAGATCTGAG TGCTGCTGCA AGTAATATTC AGTCAGATGT TACTGCATCT
    GATCCTAAAA CTAGCACACC CCTTAAAATA CTATCTGAAG GAACAAATAG CAACACTTTG
    GAAACTATGG AAAACGTAAC ATCCACAGAT ATAAACGATA ATGTTACTAT AGAGGTCATC
    TCAAATCGAT CCGAAGAAGT TCCAGCGATT CAGGATTTGG GAAAAACGAA GGACATATCT
    GAACCAACTG CGAACCCAAT CGAAGAAATT ACATCCATAC AAAATAGTAC GACTATTTCA
    GAACAATCTG AAGGGCCTCA AGAACCAGAA ATACCTATCG AAGTATCTGA AACGACAGAA
    GCACTTCAAG TCCCTCGAGA AGAAGCAAGC ATTGAAATTG TCAGCCCTCC AAACGAAGAA
    CAAACCAAAT CTCCAACATC ACAGGAAGTT AATATACAAG ACACATCACA CATCATATCT
    TTACCAATTA CTGATGTTAC ACCCACTCCA GAAATCATTA ATATAGCGCC TAGTACCTCA
    TCAATATCCA AAGAACAAAA GCAAAGTCCG AAGCGTCTTT CAAAGATTCC CGTAAGAACC
    CTGAGCAGCA GCAGTCTTCG CAGTGAGAGT CGGTCGAGCA ATCGGACTCC AACGGCCAAT
    GATGAGATCG AAAGGGAAGA GACCACGTCA CAAGGTGTAC CCATAGGGGA AACAGTCAGT
    TCTCCAAAAT CCGAACAGTT GTCCGACAAC CAGGAGGTTA ATCTTGTGAG CCAAGAAACA
    CAGTCCAAAA AGGATACAAA TATTGTCGAA GAACCTGCCA CCCAGCCTTT AGGCTTAGAG
    CTGGAAGAAC ATAGTCCGAA TGCAACTGCT GTAGCGGTCA GTCCCACCGA CTCGGACGAA
    GTATTCGAAG ATGCTCCTGA GTTCAGCGGT AGCGATGGAA CAAGACCCCA TGATGAAACC
    GCTTCCGATG CAGAGCTTTA TAGCCTTGAT AGCGATGGGC AGCGGGCTGA AACCAAGTCC
    CCAGAGGATG AAGTCGTAAT CTTGCTGGAT GAGGAATCCC AGATGGAATC TTCGATAGCC
    CAGTCCGAAA GCAACGCAAG TCTTGATTCC CACTCAAGTG AATCCGAGAC GTCCAAAGTT
    GTTCTCAAGG AGTTTGTACC CTCTGGAGAT CCGGCCAAAC AGAACCTAAG CGAACTGGTT
    GAGGACACTC AACGTTTGAT CAAGCAGATG CGCGATGAGA TCAGCATGGA TGAGTTCGAG
    TCTACCGATG AGGATGAATA CTCCGATGAA TACTCGGATG AGTATGACGA AGGGGAGGAG
    GAGGAGTGGT ACGACAGCGA GGGTGAAGAG GAGGGAGACT TCGATGGGGA GGAGGGTAAT
    ACCTATAACG AGCACGCATC CTACATCGAG GAGGCCTCCA CTGGTGACGA AGGCACCGAA
    ATAGAGGACA TAATGGAGGA GGATGAGGAT CTGGCTGATG ACGACGAGCC ACTGCAATCA
    CAAATTCCAC TAGACATTGA ACCAGTCATA TCGCCTGCTC TATCAGTCAC GCCCACCAAC
    CAAGAAACTG ATACGATCGC ACACACTGAG GTTGTTTCCT CCACAGGAAC GAGGCTGGAA
    ACAGAGTTGC CGAACCCTGC AATGGAATCC ATCTTGCCTT CTCAAAGTGT TCAGGAGGAC
    ATCAAGGTGG AAGCGATTCC TATTCAGTCC GCTCCCCCCA TAGCTGACTC GGAAACTCGA
    CCTGCAGAGC AACCAGTTGA GCTTGTGCTA GAAATCCCTT CAGAAGTTGA GCCTACTCCC
    GTTGAAGAGC CCACTGCCCT GCCCATAACA CCTGCACCAC CAATTGTTGA TTCCGAATCG
    CGACCCGTGG AGCCGCCTGT TGAAACAGTA CTGGAAGAAC CTAAAAAGGT AACCCCGAGC
    ATGAAAGGCA AGACTGCAAA CAGTGGAACT GCCTCCAAAG GTCCTTCCAC ATCAAGTTCG
    ACTAAAACAA ACAAGTCCAC AGTTAGTAAG ATTCCCAAGC CCACCAATGA ACCCACTAAC
    AAATCGAATA GCACCCCCTT GAACAAGAAG GTTCCGCTGC GCTCCAAGTC CTTCTCGGCC
    CCTATGGGCA TTTCATCCGT GAAGCGAATC CAGGAGGTCT ACCTCCAGAA GCAGAGTTCA
    TCGATTGCAA CCAGCCGAGT GCCGCTCAAG AGCAGTCCCG TCACCAAGAA GTCAATCAAC
    GATGCCATCA GCAGGTTTAA CTCCAATCAG GCCGATGGAC CCAGCACCAG TGGAGCAGCG
    GCTGCGGCAG CAGCGGCATT GTTGAAACCT CGATCCCAAC CCCGAATCCC AAAGAAGAAA
    TACCACGAGA CCTGCTTCTC TGACGATGAC TACGAGACCT CGGCCACGGA GGAGGAGCAG
    GAGGAACCCA ACTTGGCCGA GCCCCAAAAA GCAGAACAGC TGAAGCGTAA GATGAGTATG
    CCCGTTTTTC GGGCATATCC TAGTGTTCAA GAGCCGGTAA TCGAGGATCC AGCGATCCTT
    GCCCGCAAGT ACGTCGATCA GGAGCTGGTG ACCAATATCG CAGAGGCCCA GATAGCCGCC
    ACCTTGGTGA GCATGAAGTT CTCGGAAGAC GTGGCTCTGT GGGCGGCCAG GGAGTGCTCC
    GATCTGGACC AGGCCATTGC CATGCTCCAG CAGGAGTGCG AGCTCTGCAT GAACAGCTAT
    CCCATGAATC AGATGGTGTC CATGCTAAAG TGTCTGCACA AGTGCTGCAA GCAGTGTGCC
    AAGAGCTACT TTACAGTACA GATAACCGAT CGCAGTATAA ACGATTGCAG TTGCCCGTTT
    TGCAAGCTGC CCGAGTTGAG CAACGAGGCT CAGCATGAGG ATGAGCACCT CGAGTACTTC
    TCCAACCTGG ACATCTTCCT GAAGAGCATC CTGGACAACG ATGTGCACGA ACTGTTCCAG
    CGCAAGTTGC GCGATCGCTC GCTGCTGCAG GATCCCAACT TCAAGTGGTG CATCCAGTGC
    TCGTCCGGAT TCTTCGCTCG GCCCAAGCAG AAGCGATTGA TTTGTCCGGA CTGTGGATCC
    GTTACCTGTG CCCAGTGCCG AAAGCCCTGG GAGCGCCAGC ACGAGGGCAG TAGCTGCGAA
    GCTTATCTGG AATGGAAGCG CGAAAACGAT CCCGAGCTGC AGGCCCAGGG TGTCCAGGAG
    CATTTGGCCC AAAACGGCAT CGACTGTCCC AAGTGCAAGT TCCGATACTC GCTGGCCAGA
    GGCGGATGCA TGCATTTTAC CTGTACTCAG TGCAAATTCG AGTTCTGCTA CGGATGTGCC
    CGACCCTTCA TGATGGGTGC CAAGTGCACT GTGTCCACAT ACTGTGCCAA GCTCGGTCTC
    CATGCCCATC ATCCGCGCAA CTGCCTGTTC TACCTGAGGG ATAAAATCCC CCTTCAGTTG
    CAGTTCCTGC TCAAGGAGCA AAACGTCAAA TTCGACACGG AACCCATGCA GATCAAGGAC
    GAGTCCAGCA GTTCGTCCAA GGCTCGTGCC CAGGCCCGCT GCCCGATCCC GCTGCAAAAG
    GAGACGCCGC AAGGACTAGT CGACACAGTG TGCAACACGG AAGTTCCTGA CAAGCACGCT
    GGCATGTGTC GCACCCACTA CGTAGAGTAT TTGGCTGGCA AGGTTGCCAA GGCTGGCATC
    GATCCACTGC CCATCTTTGA TCTGACGGAC TGCGTCCAGG AGCTGCGAAG GCGAGGCATC
    GCTCTGCCGG AGAGAGGACC CTGGGACACC GATGAGATCT ACAAGAACAT GTGCTCCGAG
    GTAATCAAAA AGCATATACC ACTGAAATCG GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260157.1
    CDS270..8825
    Translation

    Target ORF information:

    RefSeq Version NM_001273228.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant G (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273228.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGAGT CCGTTGAAAA CATTTGGAAT
    ACTCTGGATG AGAGCATACA GGCGCAGGCG GAGCAGGTAC TCAAGAAGGC TCAGAAGGTC
    TCCACGGCCT GTGGTGGGAC TCCTCCCCGG GAAATAGCAG CTGTGGAGAT GGGAACCTCC
    CCGCCTCCAC AAAGCATTTC CACACAGACC TACGACGCTC TGCCCTTCAA TACAAAGCAA
    GAAGAGATCA TCCCCGTTGT TCCTGACCGC TTCACGACTC CAGAACCAAA CAAGATGGAG
    CGACGTCCGC ACTATCGCAG CAATTCCCAG CTGCAACAGG AGAGTGAACG TTATCGGAGT
    GCCAATGACC TGAGGTATCA CGACGGCTTT GGATTGGATC CATATAGTGC CGGTCTGGTC
    ACCAAAAGAC CCAATTTCAT AAACGAGCTA CGCATGCTGC AGCTTCAAGT ATCTTCACCT
    TTCGATATGC CGCATGAGAC ATTCGGAGTC AAGCATGAGC CGGCCAGAGA TCCGGAAACG
    GAAATGCACA TCATACTGAA AGAACTGGAG CTGTACAAGT TTACGGTTGA GGAACTGGAA
    GCTGCCCTTA AGTACTGCTC CCCGGAGACT CATCCGATTC AGTGGCTGCG CGAGAACTGG
    CACAAGCTGG TGCAGACGGT GCAGAGTTTG TCAACCAAGT ACGGACAGGA GAGGGGTGAA
    AACACGATCG GAACTGTGTC CCAAAACGAA GCACGCGAGG CATTGCGCAA CTCGGGGGGA
    AATGTGTGGC AGGCGGTGGC TGACTGCATC CAACAGAGGC AGCAGAAGTA CAGGAAACTG
    GCGGCCAAGG GAAACTTCCT GCGCGATGAC ATTGTCAACG CGCTGACTGC GCACCAAGAT
    ATCCATGACT TCTTGAACAC ACACGCCTTG GACTGCCTGC AGCCTCCGTT GGCTGGGCAA
    AGTCCCAGCC CCGCCCAAGC CAATCCTTTC GACCAACCCC GCACCGACGA GAGCCCCGTC
    AAATCCACCT ATGCCACACC CAGTCCCTAT CAGATGGAGG ACAGCACCCT GAAGAACCTG
    GAGATACTCA TTGGCAACAT GGAACAGAAC CAGGCCAAGC AGAACCAGGA GGTTCTACGT
    TCCATCGAGA CCATGCTGGA CACATTCAAG GGAAAGCCGG AACTGGAGTA CGAAACGGAT
    CCGGAGATAA TGCGTATCCT AACAAAGAGC CCCATTAGCA CAATGAAACC ATCTGGACCG
    GCAGAGGATA AGTCAACCGA TGATGTAAAG AACTTTGTAT GGCAACACAT TCAGGAAATT
    GTGCCCAACC TGGTGCAACA GGTTGAACAG GAGCTCATGG AGAAACCGGA AGAGGTAGCT
    AAAATTGAGG CAGAACAGCC AAAGGAGCCT GAACCTCTAC TCGAACCTCA GCCTGAGCCC
    ACACCAAGTG TCGATCCTGC TGTCTACATC ATGGAGGAAG TCATCAAGCC CAATTTAAGG
    GAAGCCAGCA TTCGTGAGGA AGTTCAACCC AGTTTTATCT ATGCCACTGA AATAGCCAAC
    TTCAAGTTGG AATTTGATCG TGGCACTGAA CGATGGCACG AGGCGGAATG GGAAAGCAGC
    GATCTTACGG ATGCAGAACG CATAGTATAC AAATGTTATA TGGCTCCTAA TGAGCAACCC
    AAGGAGGATG TTGTAGATGT AGCGGTTGAA AGTTCAGTCA ATTCGCTTCC AACAGCAAAA
    GCACAAGAAG AATCTGCTCC AGAAGTACCC ATTGAAGCTC AAAAGATTGA AAATACAGAA
    GTTACACAAC CAGAGACCGT TAATGAGGAA CTTCGCCAGC AGGAAAAACT AGAAACACCA
    TTAGTTATTA CAAGTGAGAC AATTAGTGAA ACAGTTTCGC AGACAGCCAA TGAAAGTGAT
    AAACAAAAAT CAATTGAAAA TAATTTGCAA ATTAAGCAGA ATGTGGCAGA AGTACAAGTT
    CAGTCGGATG ACCAGCCAAG CACGAGTAGA GATGCCAACC GAAGAGCTAA GAGATCCCAG
    CAATCGAGAA AGGGTAGATC CCGCGAGCAA AGCCAAAAAC CAACCAATCG TACAAAGCTA
    CCAAACAATA TCGATCAGAA AGTAAATGAG TCAAAAACAG CAGCTAAGGA AACAGAAGCA
    GTCAAAGATA AAGATCTGAG TGCTGCTGCA AGTAATATTC AGTCAGATGT TACTGCATCT
    GATCCTAAAA CTAGCACACC CCTTAAAATA CTATCTGAAG GAACAAATAG CAACACTTTG
    GAAACTATGG AAAACGTAAC ATCCACAGAT ATAAACGATA ATGTTACTAT AGAGGTCATC
    TCAAATCGAT CCGAAGAAGT TCCAGCGATT CAGGATTTGG GAAAAACGAA GGACATATCT
    GAACCAACTG CGAACCCAAT CGAAGAAATT ACATCCATAC AAAATAGTAC GACTATTTCA
    GAACAATCTG AAGGGCCTCA AGAACCAGAA ATACCTATCG AAGTATCTGA AACGACAGAA
    GCACTTCAAG TCCCTCGAGA AGAAGCAAGC ATTGAAATTG TCAGCCCTCC AAACGAAGAA
    CAAACCAAAT CTCCAACATC ACAGGAAGTT AATATACAAG ACACATCACA CATCATATCT
    TTACCAATTA CTGATGTTAC ACCCACTCCA GAAATCATTA ATATAGCGCC TAGTACCTCA
    TCAATATCCA AAGAACAAAA GCAAAGTCCG AAGCGTCTTT CAAAGATTCC CGTAAGAACC
    CTGAGCAGCA GCAGTCTTCG CAGTGAGAGT CGGTCGAGCA ATCGGACTCC AACGGCCAAT
    GATGAGATCG AAAGGGAAGA GACCACGTCA CAAGGTGTAC CCATAGGGGA AACAGTCAGT
    TCTCCAAAAT CCGAACAGTT GTCCGACAAC CAGGAGGTTA ATCTTGTGAG CCAAGAAACA
    CAGTCCAAAA AGGATACAAA TATTGTCGAA GAACCTGCCA CCCAGCCTTT AGGCTTAGAG
    CTGGAAGAAC ATAGTCCGAA TGCAACTGCT GTAGCGGTCA GTCCCACCGA CTCGGACGAA
    GTATTCGAAG ATGCTCCTGA GTTCAGCGGT AGCGATGGAA CAAGACCCCA TGATGAAACC
    GCTTCCGATG CAGAGCTTTA TAGCCTTGAT AGCGATGGGC AGCGGGCTGA AACCAAGTCC
    CCAGAGGATG AAGTCGTAAT CTTGCTGGAT GAGGAATCCC AGATGGAATC TTCGATAGCC
    CAGTCCGAAA GCAACGCAAG TCTTGATTCC CACTCAAGTG AATCCGAGAC GTCCAAAGTT
    GTTCTCAAGG AGTTTGTACC CTCTGGAGAT CCGGCCAAAC AGAACCTAAG CGAACTGGTT
    GAGGACACTC AACGTTTGAT CAAGCAGATG CGCGATGAGA TCAGCATGGA TGAGTTCGAG
    TCTACCGATG AGGATGAATA CTCCGATGAA TACTCGGATG AGTATGACGA AGGGGAGGAG
    GAGGAGTGGT ACGACAGCGA GGGTGAAGAG GAGGGAGACT TCGATGGGGA GGAGGGTAAT
    ACCTATAACG AGCACGCATC CTACATCGAG GAGGCCTCCA CTGGTGACGA AGGCACCGAA
    ATAGAGGACA TAATGGAGGA GGATGAGGAT CTGGCTGATG ACGACGAGCC ACTGCAATCA
    CAAATTCCAC TAGACATTGA ACCAGTCATA TCGCCTGCTC TATCAGTCAC GCCCACCAAC
    CAAGAAACTG ATACGATCGC ACACACTGAG GTTGTTTCCT CCACAGGAAC GAGGCTGGAA
    ACAGAGTTGC CGAACCCTGC AATGGAATCC ATCTTGCCTT CTCAAAGTGT TCAGGAGGAC
    ATCAAGGTGG AAGCGATTCC TATTCAGTCC GCTCCCCCCA TAGCTGACTC GGAAACTCGA
    CCTGCAGAGC AACCAGTTGA GCTTGTGCTA GAAATCCCTT CAGAAGTTGA GCCTACTCCC
    GTTGAAGAGC CCACTGCCCT GCCCATAACA CCTGCACCAC CAATTGTTGA TTCCGAATCG
    CGACCCGTGG AGCCGCCTGT TGAAACAGTA CTGGAAGAAC CTAAAAAGGT AACCCCGAGC
    ATGAAAGGCA AGACTGCAAA CAGTGGAACT GCCTCCAAAG GTCCTTCCAC ATCAAGTTCG
    ACTAAAACAA ACAAGTCCAC AGTTAGTAAG ATTCCCAAGC CCACCAATGA ACCCACTAAC
    AAATCGAATA GCACCCCCTT GAACAAGAAG GTTCCGCTGC GCTCCAAGTC CTTCTCGGCC
    CCTATGGGCA TTTCATCCGT GAAGCGAATC CAGGAGGTCT ACCTCCAGAA GCAGAGTTCA
    TCGATTGCAA CCAGCCGAGT GCCGCTCAAG AGCAGTCCCG TCACCAAGAA GTCAATCAAC
    GATGCCATCA GCAGGTTTAA CTCCAATCAG GCCGATGGAC CCAGCACCAG TGGAGCAGCG
    GCTGCGGCAG CAGCGGCATT GTTGAAACCT CGATCCCAAC CCCGAATCCC AAAGAAGAAA
    TACCACGAGA CCTGCTTCTC TGACGATGAC TACGAGACCT CGGCCACGGA GGAGGAGCAG
    GAGGAACCCA ACTTGGCCGA GCCCCAAAAA GCAGAACAGC TGAAGCGTAA GATGAGTATG
    CCCGTTTTTC GGGCATATCC TAGTGTTCAA GAGCCGGTAA TCGAGGATCC AGCGATCCTT
    GCCCGCAAGT ACGTCGATCA GGAGCTGGTG ACCAATATCG CAGAGGCCCA GATAGCCGCC
    ACCTTGGTGA GCATGAAGTT CTCGGAAGAC GTGGCTCTGT GGGCGGCCAG GGAGTGCTCC
    GATCTGGACC AGGCCATTGC CATGCTCCAG CAGGAGTGCG AGCTCTGCAT GAACAGCTAT
    CCCATGAATC AGATGGTGTC CATGCTAAAG TGTCTGCACA AGTGCTGCAA GCAGTGTGCC
    AAGAGCTACT TTACAGTACA GATAACCGAT CGCAGTATAA ACGATTGCAG TTGCCCGTTT
    TGCAAGCTGC CCGAGTTGAG CAACGAGGCT CAGCATGAGG ATGAGCACCT CGAGTACTTC
    TCCAACCTGG ACATCTTCCT GAAGAGCATC CTGGACAACG ATGTGCACGA ACTGTTCCAG
    CGCAAGTTGC GCGATCGCTC GCTGCTGCAG GATCCCAACT TCAAGTGGTG CATCCAGTGC
    TCGTCCGGAT TCTTCGCTCG GCCCAAGCAG AAGCGATTGA TTTGTCCGGA CTGTGGATCC
    GTTACCTGTG CCCAGTGCCG AAAGCCCTGG GAGCGCCAGC ACGAGGGCAG TAGCTGCGAA
    GCTTATCTGG AATGGAAGCG CGAAAACGAT CCCGAGCTGC AGGCCCAGGG TGTCCAGGAG
    CATTTGGCCC AAAACGGCAT CGACTGTCCC AAGTGCAAGT TCCGATACTC GCTGGCCAGA
    GGCGGATGCA TGCATTTTAC CTGTACTCAG TGCAAATTCG AGTTCTGCTA CGGATGTGCC
    CGACCCTTCA TGATGGGTGC CAAGTGCACT GTGTCCACAT ACTGTGCCAA GCTCGGTCTC
    CATGCCCATC ATCCGCGCAA CTGCCTGTTC TACCTGAGGG ATAAAATCCC CCTTCAGTTG
    CAGTTCCTGC TCAAGGAGCA AAACGTCAAA TTCGACACGG AACCCATGCA GATCAAGGAC
    GAGTCCAGCA GTTCGTCCAA GGCTCGTGCC CAGGCCCGCT GCCCGATCCC GCTGCAAAAG
    GAGACGCCGC AAGGACTAGT CGACACAGTG TGCAACACGG AAGTTCCTGA CAAGCACGCT
    GGCATGTGTC GCACCCACTA CGTAGAGTAT TTGGCTGGCA AGGTTGCCAA GGCTGGCATC
    GATCCACTGC CCATCTTTGA TCTGACGGAC TGCGTCCAGG AGCTGCGAAG GCGAGGCATC
    GCTCTGCCGG AGAGAGGACC CTGGGACACC GATGAGATCT ACAAGAACAT GTGCTCCGAG
    GTAATCAAAA AGCATATACC ACTGAAATCG GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa13958
    Clone ID Related Accession (Same CDS sequence) NM_001273231.2
    Accession Version NM_001273231.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273231.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGAG
    TCCGTTGAAA ACATTTGGAA TACTCTGGAT GAGAGCATAC AGGCGCAGGC GGAGCAGGTA
    CTCAAGAAGG CTCAGAAGGT CTCCACGGCC TGTGGTGGGA CTCCTCCCCG GGAAATAGCA
    GCTGTGGAGA TGGGAACCTC CCCGCCTCCA CAAAGCATTT CCACACAGAC CTACGACGCT
    CTGCCCTTCA ATACAAAGCA AGAAGAGATC ATCCCCGTTG TTCCTGACCG CTTCACGACT
    CCAGAACCAA ACAAGATGGA GCGACGTCCG CACTATCGCA GCAATTCCCA GCTGCAACAG
    GAGAGTGAAC GTTATCGGAG TGCCAATGAC CTGAGGTATC ACGACGGCTT TGGATTGGAT
    CCATATAGTG CCGGTCTGGT CACCAAAAGA CCCAATTTCA TAAACGAGCT ACGCATGCTG
    CAGCTTCAAG TATCTTCACC TTTCGATATG CCGCATGAGA CATTCGGAGT CAAGCATGAG
    CCGGCCAGAG ATCCGGAAAC GGAAATGCAC ATCATACTGA AAGAACTGGA GCTGTACAAG
    TTTACGGTTG AGGAACTGGA AGCTGCCCTT AAGTACTGCT CCCCGGAGAC TCATCCGATT
    CAGTGGCTGC GCGAGAACTG GCACAAGCTG GTGCAGACGG TGCAGAGTTT GTCAACCAAG
    TACGGACAGG AGAGGGGTGA AAACACGATC GGAACTGTGT CCCAAAACGA AGCACGCGAG
    GCATTGCGCA ACTCGGGGGG AAATGTGTGG CAGGCGGTGG CTGACTGCAT CCAACAGAGG
    CAGCAGAAGT ACAGGAAACT GGCGGCCAAG GGAAACTTCC TGCGCGATGA CATTGTCAAC
    GCGCTGACTG CGCACCAAGA TATCCATGAC TTCTTGAACA CACACGCCTT GGACTGCCTG
    CAGCCTCCGT TGGCTGGGCA AAGTCCCAGC CCCGCCCAAG CCAATCCTTT CGACCAACCC
    CGCACCGACG AGAGCCCCGT CAAATCCACC TATGCCACAC CCAGTCCCTA TCAGATGGAG
    GACAGCACCC TGAAGAACCT GGAGATACTC ATTGGCAACA TGGAACAGAA CCAGGCCAAG
    CAGAACCAGG AGGTTCTACG TTCCATCGAG ACCATGCTGG ACACATTCAA GGGAAAGCCG
    GAACTGGAGT ACGAAACGGA TCCGGAGATA ATGCGTATCC TAACAAAGAG CCCCATTAGC
    ACAATGAAAC CATCTGGACC GGCAGAGGAT AAGTCAACCG ATGATGTAAA GAACTTTGTA
    TGGCAACACA TTCAGGAAAT TGTGCCCAAC CTGGTGCAAC AGGTTGAACA GGAGCTCATG
    GAGAAACCGG AAGAGGTAGC TAAAATTGAG GCAGAACAGC CAAAGGAGCC TGAACCTCTA
    CTCGAACCTC AGCCTGAGCC CACACCAAGT GTCGATCCTG CTGTCTACAT CATGGAGGAA
    GTCATCAAGC CCAATTTAAG GGAAGCCAGC ATTCGTGAGG AAGTTCAACC CAGTTTTATC
    TATGCCACTG AAATAGCCAA CTTCAAGTTG GAATTTGATC GTGGCACTGA ACGATGGCAC
    GAGGCGGAAT GGGAAAGCAG CGATCTTACG GATGCAGAAC GCATAGTATA CAAATGTTAT
    ATGGCTCCTA ATGAGCAACC CAAGGAGGAT GTTGTAGATG TAGCGGTTGA AAGTTCAGTC
    AATTCGCTTC CAACAGCAAA AGCACAAGAA GAATCTGCTC CAGAAGTACC CATTGAAGCT
    CAAAAGATTG AAAATACAGA AGTTACACAA CCAGAGACCG TTAATGAGGA ACTTCGCCAG
    CAGGAAAAAC TAGAAACACC ATTAGTTATT ACAAGTGAGA CAATTAGTGA AACAGTTTCG
    CAGACAGCCA ATGAAAGTGA TAAACAAAAA TCAATTGAAA ATAATTTGCA AATTAAGCAG
    AATGTGGCAG AAGTACAAGT TCAGTCGGAT GACCAGCCAA GCACGAGTAG AGATGCCAAC
    CGAAGAGCTA AGAGATCCCA GCAATCGAGA AAGGGTAGAT CCCGCGAGCA AAGCCAAAAA
    CCAACCAATC GTACAAAGCT ACCAAACAAT ATCGATCAGA AAGTAAATGA GTCAAAAACA
    GCAGCTAAGG AAACAGAAGC AGTCAAAGAT AAAGATCTGA GTGCTGCTGC AAGTAATATT
    CAGTCAGATG TTACTGCATC TGATCCTAAA ACTAGCACAC CCCTTAAAAT ACTATCTGAA
    GGAACAAATA GCAACACTTT GGAAACTATG GAAAACGTAA CATCCACAGA TATAAACGAT
    AATGTTACTA TAGAGGTCAT CTCAAATCGA TCCGAAGAAG TTCCAGCGAT TCAGGATTTG
    GGAAAAACGA AGGACATATC TGAACCAACT GCGAACCCAA TCGAAGAAAT TACATCCATA
    CAAAATAGTA CGACTATTTC AGAACAATCT GAAGGGCCTC AAGAACCAGA AATACCTATC
    GAAGTATCTG AAACGACAGA AGCACTTCAA GTCCCTCGAG AAGAAGCAAG CATTGAAATT
    GTCAGCCCTC CAAACGAAGA ACAAACCAAA TCTCCAACAT CACAGGAAGT TAATATACAA
    GACACATCAC ACATCATATC TTTACCAATT ACTGATGTTA CACCCACTCC AGAAATCATT
    AATATAGCGC CTAGTACCTC ATCAATATCC AAAGAACAAA AGCAAAGTCC GAAGCGTCTT
    TCAAAGATTC CCGTAAGAAC CCTGAGCAGC AGCAGTCTTC GCAGTGAGAG TCGGTCGAGC
    AATCGGACTC CAACGGCCAA TGATGAGATC GAAAGGGAAG AGACCACGTC ACAAGGTGTA
    CCCATAGGGG AAACAGTCAG TTCTCCAAAA TCCGAACAGT TGTCCGACAA CCAGGAGGTT
    AATCTTGTGA GCCAAGAAAC ACAGTCCAAA AAGGATACAA ATATTGTCGA AGAACCTGCC
    ACCCAGCCTT TAGGCTTAGA GCTGGAAGAA CATAGTCCGA ATGCAACTGC TGTAGCGGTC
    AGTCCCACCG ACTCGGACGA AGTATTCGAA GATGCTCCTG AGTTCAGCGG TAGCGATGGA
    ACAAGACCCC ATGATGAAAC CGCTTCCGAT GCAGAGCTTT ATAGCCTTGA TAGCGATGGG
    CAGCGGGCTG AAACCAAGTC CCCAGAGGAT GAAGTCGTAA TCTTGCTGGA TGAGGAATCC
    CAGATGGAAT CTTCGATAGC CCAGTCCGAA AGCAACGCAA GTCTTGATTC CCACTCAAGT
    GAATCCGAGA CGTCCAAAGT TGTTCTCAAG GAGTTTGTAC CCTCTGGAGA TCCGGCCAAA
    CAGAACCTAA GCGAACTGGT TGAGGACACT CAACGTTTGA TCAAGCAGAT GCGCGATGAG
    ATCAGCATGG ATGAGTTCGA GTCTACCGAT GAGGATGAAT ACTCCGATGA ATACTCGGAT
    GAGTATGACG AAGGGGAGGA GGAGGAGTGG TACGACAGCG AGGGTGAAGA GGAGGGAGAC
    TTCGATGGGG AGGAGGGTAA TACCTATAAC GAGCACGCAT CCTACATCGA GGAGGCCTCC
    ACTGGTGACG AAGGCACCGA AATAGAGGAC ATAATGGAGG AGGATGAGGA TCTGGCTGAT
    GACGACGAGC CACTGCAATC ACAAATTCCA CTAGACATTG AACCAGTCAT ATCGCCTGCT
    CTATCAGTCA CGCCCACCAA CCAAGAAACT GATACGATCG CACACACTGA GGTTGTTTCC
    TCCACAGGAA CGAGGCTGGA AACAGAGTTG CCGAACCCTG CAATGGAATC CATCTTGCCT
    TCTCAAAGTG TTCAGGAGGA CATCAAGGTG GAAGCGATTC CTATTCAGTC CGCTCCCCCC
    ATAGCTGACT CGGAAACTCG ACCTGCAGAG CAACCAGTTG AGCTTGTGCT AGAAATCCCT
    TCAGAAGTTG AGCCTACTCC CGTTGAAGAG CCCACTGCCC TGCCCATAAC ACCTGCACCA
    CCAATTGTTG ATTCCGAATC GCGACCCGTG GAGCCGCCTG TTGAAACAGT ACTGGAAGAA
    CCTAAAAAGG TAACCCCGAG CATGAAAGGC AAGACTGCAA ACAGTGGAAC TGCCTCCAAA
    GGTCCTTCCA CATCAAGTTC GACTAAAACA AACAAGTCCA CAGTTAGTAA GATTCCCAAG
    CCCACCAATG AACCCACTAA CAAATCGAAT AGCACCCCCT TGAACAAGAA GGTTCCGCTG
    CGCTCCAAGT CCTTCTCGGC CCCTATGGGC ATTTCATCCG TGAAGCGAAT CCAGGAGGTC
    TACCTCCAGA AGCAGAGTTC ATCGATTGCA ACCAGCCGAG TGCCGCTCAA GAGCAGTCCC
    GTCACCAAGA AGTCAATCAA CGATGCCATC AGCAGGTTTA ACTCCAATCA GGCCGATGGA
    CCCAGCACCA GTGGAGCAGC GGCTGCGGCA GCAGCGGCAT TGTTGAAACC TCGATCCCAA
    CCCCGAATCC CAAAGAAGAA ATACCACGAG ACCTGCTTCT CTGACGATGA CTACGAGACC
    TCGGCCACGG AGGAGGAGCA GGAGGAACCC AACTTGGCCG AGCCCCAAAA AGCAGAACAG
    CTGAAGCGTA AGATGAGTAT GCCCGTTTTT CGGGCATATC CTAGTGTTCA AGAGCCGGTA
    ATCGAGGATC CAGCGATCCT TGCCCGCAAG TACGTCGATC AGGAGCTGGT GACCAATATC
    GCAGAGGCCC AGATAGCCGC CACCTTGGTG AGCATGAAGT TCTCGGAAGA CGTGGCTCTG
    TGGGCGGCCA GGGAGTGCTC CGATCTGGAC CAGGCCATTG CCATGCTCCA GCAGGAGTGC
    GAGCTCTGCA TGAACAGCTA TCCCATGAAT CAGATGGTGT CCATGCTAAA GTGTCTGCAC
    AAGTGCTGCA AGCAGTGTGC CAAGAGCTAC TTTACAGTAC AGATAACCGA TCGCAGTATA
    AACGATTGCA GTTGCCCGTT TTGCAAGCTG CCCGAGTTGA GCAACGAGGC TCAGCATGAG
    GATGAGCACC TCGAGTACTT CTCCAACCTG GACATCTTCC TGAAGAGCAT CCTGGACAAC
    GATGTGCACG AACTGTTCCA GCGCAAGTTG CGCGATCGCT CGCTGCTGCA GGATCCCAAC
    TTCAAGTGGT GCATCCAGTG CTCGTCCGGA TTCTTCGCTC GGCCCAAGCA GAAGCGATTG
    ATTTGTCCGG ACTGTGGATC CGTTACCTGT GCCCAGTGCC GAAAGCCCTG GGAGCGCCAG
    CACGAGGGCA GTAGCTGCGA AGCTTATCTG GAATGGAAGC GCGAAAACGA TCCCGAGCTG
    CAGGCCCAGG GTGTCCAGGA GCATTTGGCC CAAAACGGCA TCGACTGTCC CAAGTGCAAG
    TTCCGATACT CGCTGGCCAG AGGCGGATGC ATGCATTTTA CCTGTACTCA GTGCAAATTC
    GAGTTCTGCT ACGGATGTGC CCGACCCTTC ATGATGGGTG CCAAGTGCAC TGTGTCCACA
    TACTGTGCCA AGCTCGGTCT CCATGCCCAT CATCCGCGCA ACTGCCTGTT CTACCTGAGG
    GATAAAATCC CCCTTCAGTT GCAGTTCCTG CTCAAGGAGC AAAACGTCAA ATTCGACACG
    GAACCCATGC AGATCAAGGA CGAGTCCAGC AGTTCGTCCA AGGCTCGTGC CCAGGCCCGC
    TGCCCGATCC CGCTGCAAAA GGAGACGCCG CAAGGACTAG TCGACACAGT GTGCAACACG
    GAAGTTCCTG ACAAGCACGC TGGCATGTGT CGCACCCACT ACGTAGAGTA TTTGGCTGGC
    AAGGTTGCCA AGGCTGGCAT CGATCCACTG CCCATCTTTG ATCTGACGGA CTGCGTCCAG
    GAGCTGCGAA GGCGAGGCAT CGCTCTGCCG GAGAGAGGAC CCTGGGACAC CGATGAGATC
    TACAAGAACA TGTGCTCCGA GGTAATCAAA AAGCATATAC CACTGAAATC GGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001260160.1
    CDS149..8545
    Translation

    Target ORF information:

    RefSeq Version NM_001273231.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant J (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001273231.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGGC GGCCGGCGCC CAGCGGCGAC AGATGAGCAC ATCGGTGTTC
    AATCTCAACT CGAATCCACG TCGACCACTG TCCGAGGCCC AAAACGGAGG TGCCTGGCTC
    GCCAATCAGC GCATCCAGCA GGCCCAATCC CTGGCGCAGC TAAACTGCGC TGGATGTCAA
    CAGAGTCAGC AGCATCCTGG CTGGGCACAG CATCCGCACC AAGCACTTCC TCAGCACCAG
    CATCCCGATC AGTGGTCACA GTTCGGCTCC CAACAGCAAT TCAACAACTC CAATCTGTCC
    CTGAACGTTG GACCGGGTTA CATGTCGCAG CAACATCATC CACACTATCC GCCACCAGTG
    TTTATGACCC AGCGTGGTAT GATGCCCAAT GTGTATCCAG GAGCACCAGG CTATCCCATG
    ATGCATCCAG GTGTCATGGG AATGCCACCT TCAGCTGCCT CCAGAGCTGC ATCCCGTTCC
    CGGTACGCTG CATCACCAAC ACCCAGCCGC AAATCGATGT CCTTGCGTCG CAAGCGCAAT
    AGCTACGTGG ATGACGAACT TACGGATGAC GAGGACTCCG ACCAGGATGA CCGCAGATCT
    TTGGTGTCCA ATCGCTCGGG CATGACTAGT GCCTCGCGCT CCCAGCATCA CCAAAACCAT
    ATCCAGCCCC GCCAAAGGCG CCTGTCCAGT GCCTCCCAAC TCATAGCCAG CGATGAATTG
    GACGGCGATC AGGTGCATCA CAAGATGCGG AATCGTCGGG GATCCATTGC CAAGTCCGTG
    CAGAGTGAGT GGCTACCGGA GCGACGGGAA AATGAGGGAA CCCTTACCAG AAACAAAACA
    GCAACGGACT CGGCCAGAAC CAGTCGCATT TACTCCGATC TGGAGTCTGA GGGATCGGGT
    GCTCGGGCCT TGGTTCAAGC TAAAATTCAG CAGAAGCTCC AAGAAGCCGA TCAGCACAAA
    TCCTCGAAGA AGGCTGAGCC AAAACGCAAG CCGGAGATGA AGGACGAGAA TACACAGGCG
    GCAGCGGTGG TGCAAAAGGT GGTGGTGCCA CCAGCCCATG AAGAGAGTGC CTCGGAATAC
    GAGGAAGTGG TCGAAGAGGT AACCGCCTCA GAGAGTGAGG CTGAGGCTCA AACAGCACCC
    GATCCCCAGG AAGTTCCTGA CGAGATTGGG GCTGATGATC TCGGCCCACC ACCTTCTACC
    CCAGACCACG AATGGGAATG CGAGTTCTGC ACGTTTGTGA ACGAGCCCAA CATCAAGATC
    TGCTCCATAT GCTGCAAGAC GCCCAGCAAA CCTCCAGTGC AGCCCAACAA GGCCAAAAAG
    GTAGAGGAAA AACCACAGCC TCCAGCTGAA AAGTCTAACA ATATCAAGGC GTCTTCCAAG
    ACGGAAACTA AGCCAGTGCA AAAGCCTACT ACAAAGAGCC AACAACCCAG TCAGAAATCC
    GTTGCTGCTC TTTCGAAGAC TACTCACACC AACTCCACGT CTAGCTCAAA AGCGTCGCCA
    GCTGTAAACT CCAAGACCAC CTCCAGTATT CCTATCAAGA CACCATCGAA ATCCACACTG
    AAAACTTCGT CGGAAAATGA ATCCGATAAC TCGCTGGCCA AGAGTCTTCT GCACAAAGAG
    TCCGTTGAAA ACATTTGGAA TACTCTGGAT GAGAGCATAC AGGCGCAGGC GGAGCAGGTA
    CTCAAGAAGG CTCAGAAGGT CTCCACGGCC TGTGGTGGGA CTCCTCCCCG GGAAATAGCA
    GCTGTGGAGA TGGGAACCTC CCCGCCTCCA CAAAGCATTT CCACACAGAC CTACGACGCT
    CTGCCCTTCA ATACAAAGCA AGAAGAGATC ATCCCCGTTG TTCCTGACCG CTTCACGACT
    CCAGAACCAA ACAAGATGGA GCGACGTCCG CACTATCGCA GCAATTCCCA GCTGCAACAG
    GAGAGTGAAC GTTATCGGAG TGCCAATGAC CTGAGGTATC ACGACGGCTT TGGATTGGAT
    CCATATAGTG CCGGTCTGGT CACCAAAAGA CCCAATTTCA TAAACGAGCT ACGCATGCTG
    CAGCTTCAAG TATCTTCACC TTTCGATATG CCGCATGAGA CATTCGGAGT CAAGCATGAG
    CCGGCCAGAG ATCCGGAAAC GGAAATGCAC ATCATACTGA AAGAACTGGA GCTGTACAAG
    TTTACGGTTG AGGAACTGGA AGCTGCCCTT AAGTACTGCT CCCCGGAGAC TCATCCGATT
    CAGTGGCTGC GCGAGAACTG GCACAAGCTG GTGCAGACGG TGCAGAGTTT GTCAACCAAG
    TACGGACAGG AGAGGGGTGA AAACACGATC GGAACTGTGT CCCAAAACGA AGCACGCGAG
    GCATTGCGCA ACTCGGGGGG AAATGTGTGG CAGGCGGTGG CTGACTGCAT CCAACAGAGG
    CAGCAGAAGT ACAGGAAACT GGCGGCCAAG GGAAACTTCC TGCGCGATGA CATTGTCAAC
    GCGCTGACTG CGCACCAAGA TATCCATGAC TTCTTGAACA CACACGCCTT GGACTGCCTG
    CAGCCTCCGT TGGCTGGGCA AAGTCCCAGC CCCGCCCAAG CCAATCCTTT CGACCAACCC
    CGCACCGACG AGAGCCCCGT CAAATCCACC TATGCCACAC CCAGTCCCTA TCAGATGGAG
    GACAGCACCC TGAAGAACCT GGAGATACTC ATTGGCAACA TGGAACAGAA CCAGGCCAAG
    CAGAACCAGG AGGTTCTACG TTCCATCGAG ACCATGCTGG ACACATTCAA GGGAAAGCCG
    GAACTGGAGT ACGAAACGGA TCCGGAGATA ATGCGTATCC TAACAAAGAG CCCCATTAGC
    ACAATGAAAC CATCTGGACC GGCAGAGGAT AAGTCAACCG ATGATGTAAA GAACTTTGTA
    TGGCAACACA TTCAGGAAAT TGTGCCCAAC CTGGTGCAAC AGGTTGAACA GGAGCTCATG
    GAGAAACCGG AAGAGGTAGC TAAAATTGAG GCAGAACAGC CAAAGGAGCC TGAACCTCTA
    CTCGAACCTC AGCCTGAGCC CACACCAAGT GTCGATCCTG CTGTCTACAT CATGGAGGAA
    GTCATCAAGC CCAATTTAAG GGAAGCCAGC ATTCGTGAGG AAGTTCAACC CAGTTTTATC
    TATGCCACTG AAATAGCCAA CTTCAAGTTG GAATTTGATC GTGGCACTGA ACGATGGCAC
    GAGGCGGAAT GGGAAAGCAG CGATCTTACG GATGCAGAAC GCATAGTATA CAAATGTTAT
    ATGGCTCCTA ATGAGCAACC CAAGGAGGAT GTTGTAGATG TAGCGGTTGA AAGTTCAGTC
    AATTCGCTTC CAACAGCAAA AGCACAAGAA GAATCTGCTC CAGAAGTACC CATTGAAGCT
    CAAAAGATTG AAAATACAGA AGTTACACAA CCAGAGACCG TTAATGAGGA ACTTCGCCAG
    CAGGAAAAAC TAGAAACACC ATTAGTTATT ACAAGTGAGA CAATTAGTGA AACAGTTTCG
    CAGACAGCCA ATGAAAGTGA TAAACAAAAA TCAATTGAAA ATAATTTGCA AATTAAGCAG
    AATGTGGCAG AAGTACAAGT TCAGTCGGAT GACCAGCCAA GCACGAGTAG AGATGCCAAC
    CGAAGAGCTA AGAGATCCCA GCAATCGAGA AAGGGTAGAT CCCGCGAGCA AAGCCAAAAA
    CCAACCAATC GTACAAAGCT ACCAAACAAT ATCGATCAGA AAGTAAATGA GTCAAAAACA
    GCAGCTAAGG AAACAGAAGC AGTCAAAGAT AAAGATCTGA GTGCTGCTGC AAGTAATATT
    CAGTCAGATG TTACTGCATC TGATCCTAAA ACTAGCACAC CCCTTAAAAT ACTATCTGAA
    GGAACAAATA GCAACACTTT GGAAACTATG GAAAACGTAA CATCCACAGA TATAAACGAT
    AATGTTACTA TAGAGGTCAT CTCAAATCGA TCCGAAGAAG TTCCAGCGAT TCAGGATTTG
    GGAAAAACGA AGGACATATC TGAACCAACT GCGAACCCAA TCGAAGAAAT TACATCCATA
    CAAAATAGTA CGACTATTTC AGAACAATCT GAAGGGCCTC AAGAACCAGA AATACCTATC
    GAAGTATCTG AAACGACAGA AGCACTTCAA GTCCCTCGAG AAGAAGCAAG CATTGAAATT
    GTCAGCCCTC CAAACGAAGA ACAAACCAAA TCTCCAACAT CACAGGAAGT TAATATACAA
    GACACATCAC ACATCATATC TTTACCAATT ACTGATGTTA CACCCACTCC AGAAATCATT
    AATATAGCGC CTAGTACCTC ATCAATATCC AAAGAACAAA AGCAAAGTCC GAAGCGTCTT
    TCAAAGATTC CCGTAAGAAC CCTGAGCAGC AGCAGTCTTC GCAGTGAGAG TCGGTCGAGC
    AATCGGACTC CAACGGCCAA TGATGAGATC GAAAGGGAAG AGACCACGTC ACAAGGTGTA
    CCCATAGGGG AAACAGTCAG TTCTCCAAAA TCCGAACAGT TGTCCGACAA CCAGGAGGTT
    AATCTTGTGA GCCAAGAAAC ACAGTCCAAA AAGGATACAA ATATTGTCGA AGAACCTGCC
    ACCCAGCCTT TAGGCTTAGA GCTGGAAGAA CATAGTCCGA ATGCAACTGC TGTAGCGGTC
    AGTCCCACCG ACTCGGACGA AGTATTCGAA GATGCTCCTG AGTTCAGCGG TAGCGATGGA
    ACAAGACCCC ATGATGAAAC CGCTTCCGAT GCAGAGCTTT ATAGCCTTGA TAGCGATGGG
    CAGCGGGCTG AAACCAAGTC CCCAGAGGAT GAAGTCGTAA TCTTGCTGGA TGAGGAATCC
    CAGATGGAAT CTTCGATAGC CCAGTCCGAA AGCAACGCAA GTCTTGATTC CCACTCAAGT
    GAATCCGAGA CGTCCAAAGT TGTTCTCAAG GAGTTTGTAC CCTCTGGAGA TCCGGCCAAA
    CAGAACCTAA GCGAACTGGT TGAGGACACT CAACGTTTGA TCAAGCAGAT GCGCGATGAG
    ATCAGCATGG ATGAGTTCGA GTCTACCGAT GAGGATGAAT ACTCCGATGA ATACTCGGAT
    GAGTATGACG AAGGGGAGGA GGAGGAGTGG TACGACAGCG AGGGTGAAGA GGAGGGAGAC
    TTCGATGGGG AGGAGGGTAA TACCTATAAC GAGCACGCAT CCTACATCGA GGAGGCCTCC
    ACTGGTGACG AAGGCACCGA AATAGAGGAC ATAATGGAGG AGGATGAGGA TCTGGCTGAT
    GACGACGAGC CACTGCAATC ACAAATTCCA CTAGACATTG AACCAGTCAT ATCGCCTGCT
    CTATCAGTCA CGCCCACCAA CCAAGAAACT GATACGATCG CACACACTGA GGTTGTTTCC
    TCCACAGGAA CGAGGCTGGA AACAGAGTTG CCGAACCCTG CAATGGAATC CATCTTGCCT
    TCTCAAAGTG TTCAGGAGGA CATCAAGGTG GAAGCGATTC CTATTCAGTC CGCTCCCCCC
    ATAGCTGACT CGGAAACTCG ACCTGCAGAG CAACCAGTTG AGCTTGTGCT AGAAATCCCT
    TCAGAAGTTG AGCCTACTCC CGTTGAAGAG CCCACTGCCC TGCCCATAAC ACCTGCACCA
    CCAATTGTTG ATTCCGAATC GCGACCCGTG GAGCCGCCTG TTGAAACAGT ACTGGAAGAA
    CCTAAAAAGG TAACCCCGAG CATGAAAGGC AAGACTGCAA ACAGTGGAAC TGCCTCCAAA
    GGTCCTTCCA CATCAAGTTC GACTAAAACA AACAAGTCCA CAGTTAGTAA GATTCCCAAG
    CCCACCAATG AACCCACTAA CAAATCGAAT AGCACCCCCT TGAACAAGAA GGTTCCGCTG
    CGCTCCAAGT CCTTCTCGGC CCCTATGGGC ATTTCATCCG TGAAGCGAAT CCAGGAGGTC
    TACCTCCAGA AGCAGAGTTC ATCGATTGCA ACCAGCCGAG TGCCGCTCAA GAGCAGTCCC
    GTCACCAAGA AGTCAATCAA CGATGCCATC AGCAGGTTTA ACTCCAATCA GGCCGATGGA
    CCCAGCACCA GTGGAGCAGC GGCTGCGGCA GCAGCGGCAT TGTTGAAACC TCGATCCCAA
    CCCCGAATCC CAAAGAAGAA ATACCACGAG ACCTGCTTCT CTGACGATGA CTACGAGACC
    TCGGCCACGG AGGAGGAGCA GGAGGAACCC AACTTGGCCG AGCCCCAAAA AGCAGAACAG
    CTGAAGCGTA AGATGAGTAT GCCCGTTTTT CGGGCATATC CTAGTGTTCA AGAGCCGGTA
    ATCGAGGATC CAGCGATCCT TGCCCGCAAG TACGTCGATC AGGAGCTGGT GACCAATATC
    GCAGAGGCCC AGATAGCCGC CACCTTGGTG AGCATGAAGT TCTCGGAAGA CGTGGCTCTG
    TGGGCGGCCA GGGAGTGCTC CGATCTGGAC CAGGCCATTG CCATGCTCCA GCAGGAGTGC
    GAGCTCTGCA TGAACAGCTA TCCCATGAAT CAGATGGTGT CCATGCTAAA GTGTCTGCAC
    AAGTGCTGCA AGCAGTGTGC CAAGAGCTAC TTTACAGTAC AGATAACCGA TCGCAGTATA
    AACGATTGCA GTTGCCCGTT TTGCAAGCTG CCCGAGTTGA GCAACGAGGC TCAGCATGAG
    GATGAGCACC TCGAGTACTT CTCCAACCTG GACATCTTCC TGAAGAGCAT CCTGGACAAC
    GATGTGCACG AACTGTTCCA GCGCAAGTTG CGCGATCGCT CGCTGCTGCA GGATCCCAAC
    TTCAAGTGGT GCATCCAGTG CTCGTCCGGA TTCTTCGCTC GGCCCAAGCA GAAGCGATTG
    ATTTGTCCGG ACTGTGGATC CGTTACCTGT GCCCAGTGCC GAAAGCCCTG GGAGCGCCAG
    CACGAGGGCA GTAGCTGCGA AGCTTATCTG GAATGGAAGC GCGAAAACGA TCCCGAGCTG
    CAGGCCCAGG GTGTCCAGGA GCATTTGGCC CAAAACGGCA TCGACTGTCC CAAGTGCAAG
    TTCCGATACT CGCTGGCCAG AGGCGGATGC ATGCATTTTA CCTGTACTCA GTGCAAATTC
    GAGTTCTGCT ACGGATGTGC CCGACCCTTC ATGATGGGTG CCAAGTGCAC TGTGTCCACA
    TACTGTGCCA AGCTCGGTCT CCATGCCCAT CATCCGCGCA ACTGCCTGTT CTACCTGAGG
    GATAAAATCC CCCTTCAGTT GCAGTTCCTG CTCAAGGAGC AAAACGTCAA ATTCGACACG
    GAACCCATGC AGATCAAGGA CGAGTCCAGC AGTTCGTCCA AGGCTCGTGC CCAGGCCCGC
    TGCCCGATCC CGCTGCAAAA GGAGACGCCG CAAGGACTAG TCGACACAGT GTGCAACACG
    GAAGTTCCTG ACAAGCACGC TGGCATGTGT CGCACCCACT ACGTAGAGTA TTTGGCTGGC
    AAGGTTGCCA AGGCTGGCAT CGATCCACTG CCCATCTTTG ATCTGACGGA CTGCGTCCAG
    GAGCTGCGAA GGCGAGGCAT CGCTCTGCCG GAGAGAGGAC CCTGGGACAC CGATGAGATC
    TACAAGAACA TGTGCTCCGA GGTAATCAAA AAGCATATAC CACTGAAATC GGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa16329
    Clone ID Related Accession (Same CDS sequence) NM_164709.4
    Accession Version NM_164709.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product linear ubiquitin E3 ligase, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164709.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGAGT CCGTTGAAAA CATTTGGAAT
    ACTCTGGATG AGAGCATACA GGCGCAGGCG GAGCAGGTAC TCAAGAAGGC TCAGAAGGTC
    TCCACGGCCT GTGGTGGGAC TCCTCCCCGG GAAATAGCAG CTGTGGAGAT GGGAACCTCC
    CCGCCTCCAC AAAGCATTTC CACACAGACC TACGACGCTC TGCCCTTCAA TACAAAGCAA
    GAAGAGATCA TCCCCGTTGT TCCTGACCGC TTCACGACTC CAGAACCAAA CAAGATGGAG
    CGACGTCCGC ACTATCGCAG CAATTCCCAG CTGCAACAGG AGAGTGAACG TTATCGGAGT
    GCCAATGACC TGAGGTATCA CGACGGCTTT GGATTGGATC CATATAGTGC CGGTCTGGTC
    ACCAAAAGAC CCAATTTCAT AAACGAGCTA CGCATGCTGC AGCTTCAAGT ATCTTCACCT
    TTCGATATGC CGCATGAGAC ATTCGGAGTC AAGCATGAGC CGGCCAGAGA TCCGGAAACG
    GAAATGCACA TCATACTGAA AGAACTGGAG CTGTACAAGT TTACGGTTGA GGAACTGGAA
    GCTGCCCTTA AGTACTGCTC CCCGGAGACT CATCCGATTC AGTGGCTGCG CGAGAACTGG
    CACAAGCTGG TGCAGACGGT GCAGAGTTTG TCAACCAAGT ACGGACAGGA GAGGGGTGAA
    AACACGATCG GAACTGTGTC CCAAAACGAA GCACGCGAGG CATTGCGCAA CTCGGGGGGA
    AATGTGTGGC AGGCGGTGGC TGACTGCATC CAACAGAGGC AGCAGAAGTA CAGGAAACTG
    GCGGCCAAGG GAAACTTCCT GCGCGATGAC ATTGTCAACG CGCTGACTGC GCACCAAGGT
    AATGTGGAGC AGGCGCTGGT GGAGCTGAAT CGCACGCAAC TCAAGCCATT TCTAATGCGC
    ATCTGGGGCT CGCCGAATGG CGTTGAGAAT GAGAGTGGCG TGGCCATAGA CACCAAGTCG
    GATATCCATG ACTTCTTGAA CACACACGCC TTGGACTGCC TGCAGCCTCC GTTGGCTGGG
    CAAAGTCCCA GCCCCGCCCA AGCCAATCCT TTCGACCAAC CCCGCACCGA CGAGAGCCCC
    GTCAAATCCA CCTATGCCAC ACCCAGTCCC TATCAGATGG AGGACAGCAC CCTGAAGAAC
    CTGGAGATAC TCATTGGCAA CATGGAACAG AACCAGGCCA AGCAGAACCA GGAGGTTCTA
    CGTTCCATCG AGACCATGCT GGACACATTC AAGGGAAAGC CGGAACTGGA GTACGAAACG
    GATCCGGAGA TAATGCGTAT CCTAACAAAG AGCCCCATTA GCACAATGAA ACCATCTGGA
    CCGGCAGAGG ATAAGTCAAC CGATGATGTA AAGAACTTTG TATGGCAACA CATTCAGGAA
    ATTGTGCCCA ACCTGGTGCA ACAGGTTGAA CAGGAGCTCA TGGAGAAACC GGAAGAGGTA
    GCTAAAATTG AGGCAGAACA GCCAAAGGAG CCTGAACCTC TACTCGAACC TCAGCCTGAG
    CCCACACCAA GTGTCGATCC TGCTGTCTAC ATCATGGAGG AAGTCATCAA GCCCAATTTA
    AGGGAAGCCA GCATTCGTGA GGAAGTTCAA CCCAGTTTTA TCTATGCCAC TGAAATAGCC
    AACTTCAAGT TGGAATTTGA TCGTGGCACT GAACGATGGC ACGAGGCGGA ATGGGAAAGC
    AGCGATCTTA CGGATGCAGA ACGCATAGTA TACAAATGTT ATATGGCTCC TAATGAGCAA
    CCCAAGGAGG ATGTTGTAGA TGTAGCGGTT GAAAGTTCAG TCAATTCGCT TCCAACAGCA
    AAAGCACAAG AAGAATCTGC TCCAGAAGTA CCCATTGAAG CTCAAAAGAT TGAAAATACA
    GAAGTTACAC AACCAGAGAC CGTTAATGAG GAACTTCGCC AGCAGGAAAA ACTAGAAACA
    CCATTAGTTA TTACAAGTGA GACAATTAGT GAAACAGTTT CGCAGACAGC CAATGAAAGT
    GATAAACAAA AATCAATTGA AAATAATTTG CAAATTAAGC AGAATGTGGC AGAAGTACAA
    GTTCAGTCGG ATGACCAGCC AAGCACGAGT AGAGATGCCA ACCGAAGAGC TAAGAGATCC
    CAGCAATCGA GAAAGGGTAG ATCCCGCGAG CAAAGCCAAA AACCAACCAA TCGTACAAAG
    CTACCAAACA ATATCGATCA GAAAGTAAAT GAGTCAAAAA CAGCAGCTAA GGAAACAGAA
    GCAGTCAAAG ATAAAGATCT GAGTGCTGCT GCAAGTAATA TTCAGTCAGA TGTTACTGCA
    TCTGATCCTA AAACTAGCAC ACCCCTTAAA ATACTATCTG AAGGAACAAA TAGCAACACT
    TTGGAAACTA TGGAAAACGT AACATCCACA GATATAAACG ATAATGTTAC TATAGAGGTC
    ATCTCAAATC GATCCGAAGA AGTTCCAGCG ATTCAGGATT TGGGAAAAAC GAAGGACATA
    TCTGAACCAA CTGCGAACCC AATCGAAGAA ATTACATCCA TACAAAATAG TACGACTATT
    TCAGAACAAT CTGAAGGGCC TCAAGAACCA GAAATACCTA TCGAAGTATC TGAAACGACA
    GAAGCACTTC AAGTCCCTCG AGAAGAAGCA AGCATTGAAA TTGTCAGCCC TCCAAACGAA
    GAACAAACCA AATCTCCAAC ATCACAGGAA GTTAATATAC AAGACACATC ACACATCATA
    TCTTTACCAA TTACTGATGT TACACCCACT CCAGAAATCA TTAATATAGC GCCTAGTACC
    TCATCAATAT CCAAAGAACA AAAGCAAAGT CCGAAGCGTC TTTCAAAGAT TCCCGTAAGA
    ACCCTGAGCA GCAGCAGTCT TCGCAGTGAG AGTCGGTCGA GCAATCGGAC TCCAACGGCC
    AATGATGAGA TCGAAAGGGA AGAGACCACG TCACAAGGTG TACCCATAGG GGAAACAGTC
    AGTTCTCCAA AATCCGAACA GTTGTCCGAC AACCAGGAGG TTAATCTTGT GAGCCAAGAA
    ACACAGTCCA AAAAGGATAC AAATATTGTC GAAGAACCTG CCACCCAGCC TTTAGGCTTA
    GAGCTGGAAG AACATAGTCC GAATGCAACT GCTGTAGCGG TCAGTCCCAC CGACTCGGAC
    GAAGTATTCG AAGATGCTCC TGAGTTCAGC GGTAGCGATG GAACAAGACC CCATGATGAA
    ACCGCTTCCG ATGCAGAGCT TTATAGCCTT GATAGCGATG GGCAGCGGGC TGAAACCAAG
    TCCCCAGAGG ATGAAGTCGT AATCTTGCTG GATGAGGAAT CCCAGATGGA ATCTTCGATA
    GCCCAGTCCG AAAGCAACGC AAGTCTTGAT TCCCACTCAA GTGAATCCGA GACGTCCAAA
    GTTGTTCTCA AGGAGTTTGT ACCCTCTGGA GATCCGGCCA AACAGAACCT AAGCGAACTG
    GTTGAGGACA CTCAACGTTT GATCAAGCAG ATGCGCGATG AGATCAGCAT GGATGAGTTC
    GAGTCTACCG ATGAGGATGA ATACTCCGAT GAATACTCGG ATGAGTATGA CGAAGGGGAG
    GAGGAGGAGT GGTACGACAG CGAGGGTGAA GAGGAGGGAG ACTTCGATGG GGAGGAGGGT
    AATACCTATA ACGAGCACGC ATCCTACATC GAGGAGGCCT CCACTGGTGA CGAAGGCACC
    GAAATAGAGG ACATAATGGA GGAGGATGAG GATCTGGCTG ATGACGACGA GCCACTGCAA
    TCACAAATTC CACTAGACAT TGAACCAGTC ATATCGCCTG CTCTATCAGT CACGCCCACC
    AACCAAGAAA CTGATACGAT CGCACACACT GAGGTTGTTT CCTCCACAGG AACGAGGCTG
    GAAACAGAGT TGCCGAACCC TGCAATGGAA TCCATCTTGC CTTCTCAAAG TGTTCAGGAG
    GACATCAAGG TGGAAGCGAT TCCTATTCAG TCCGCTCCCC CCATAGCTGA CTCGGAAACT
    CGACCTGCAG AGCAACCAGT TGAGCTTGTG CTAGAAATCC CTTCAGAAGT TGAGCCTACT
    CCCGTTGAAG AGCCCACTGC CCTGCCCATA ACACCTGCAC CACCAATTGT TGATTCCGAA
    TCGCGACCCG TGGAGCCGCC TGTTGAAACA GTACTGGAAG AACCTAAAAA GGTAACCCCG
    AGCATGAAAG GCAAGACTGC AAACAGTGGA ACTGCCTCCA AAGGTCCTTC CACATCAAGT
    TCGACTAAAA CAAACAAGTC CACAGTTAGT AAGATTCCCA AGCCCACCAA TGAACCCACT
    AACAAATCGA ATAGCACCCC CTTGAACAAG AAGGTTCCGC TGCGCTCCAA GTCCTTCTCG
    GCCCCTATGG GCATTTCATC CGTGAAGCGA ATCCAGGAGG TCTACCTCCA GAAGCAGAGT
    TCATCGATTG CAACCAGCCG AGTGCCGCTC AAGAGCAGTC CCGTCACCAA GAAGTCAATC
    AACGATGCCA TCAGCAGGTT TAACTCCAAT CAGGCCGATG GACCCAGCAC CAGTGGAGCA
    GCGGCTGCGG CAGCAGCGGC ATTGTTGAAA CCTCGATCCC AACCCCGAAT CCCAAAGAAG
    AAATACCACG AGACCTGCTT CTCTGACGAT GACTACGAGA CCTCGGCCAC GGAGGAGGAG
    CAGGAGGAAC CCAACTTGGC CGAGCCCCAA AAAGCAGAAC AGCTGAAGCG TAAGATGAGT
    ATGCCCGTTT TTCGGGCATA TCCTAGTGTT CAAGAGCCGG TAATCGAGGA TCCAGCGATC
    CTTGCCCGCA AGTACGTCGA TCAGGAGCTG GTGACCAATA TCGCAGAGGC CCAGATAGCC
    GCCACCTTGG TGAGCATGAA GTTCTCGGAA GACGTGGCTC TGTGGGCGGC CAGGGAGTGC
    TCCGATCTGG ACCAGGCCAT TGCCATGCTC CAGCAGGAGT GCGAGCTCTG CATGAACAGC
    TATCCCATGA ATCAGATGGT GTCCATGCTA AAGTGTCTGC ACAAGTGCTG CAAGCAGTGT
    GCCAAGAGCT ACTTTACAGT ACAGATAACC GATCGCAGTA TAAACGATTG CAGTTGCCCG
    TTTTGCAAGC TGCCCGAGTT GAGCAACGAG GCTCAGCATG AGGATGAGCA CCTCGAGTAC
    TTCTCCAACC TGGACATCTT CCTGAAGAGC ATCCTGGACA ACGATGTGCA CGAACTGTTC
    CAGCGCAAGT TGCGCGATCG CTCGCTGCTG CAGGATCCCA ACTTCAAGTG GTGCATCCAG
    TGCTCGTCCG GATTCTTCGC TCGGCCCAAG CAGAAGCGAT TGATTTGTCC GGACTGTGGA
    TCCGTTACCT GTGCCCAGTG CCGAAAGCCC TGGGAGCGCC AGCACGAGGG CAGTAGCTGC
    GAAGCTTATC TGGAATGGAA GCGCGAAAAC GATCCCGAGC TGCAGGCCCA GGGTGTCCAG
    GAGCATTTGG CCCAAAACGG CATCGACTGT CCCAAGTGCA AGTTCCGATA CTCGCTGGCC
    AGAGGCGGAT GCATGCATTT TACCTGTACT CAGTGCAAAT TCGAGTTCTG CTACGGATGT
    GCCCGACCCT TCATGATGGG TGCCAAGTGC ACTGTGTCCA CATACTGTGC CAAGCTCGGT
    CTCCATGCCC ATCATCCGCG CAACTGCCTG TTCTACCTGA GGGATAAAAT CCCCCTTCAG
    TTGCAGTTCC TGCTCAAGGA GCAAAACGTC AAATTCGACA CGGAACCCAT GCAGATCAAG
    GACGAGTCCA GCAGTTCGTC CAAGGCTCGT GCCCAGGCCC GCTGCCCGAT CCCGCTGCAA
    AAGGAGACGC CGCAAGGACT AGTCGACACA GTGTGCAACA CGGAAGTTCC TGACAAGCAC
    GCTGGCATGT GTCGCACCCA CTACGTAGAG TATTTGGCTG GCAAGGTTGC CAAGGCTGGC
    ATCGATCCAC TGCCCATCTT TGATCTGACG GACTGCGTCC AGGAGCTGCG AAGGCGAGGC
    ATCGCTCTGC CGGAGAGAGG ACCCTGGGAC ACCGATGAGA TCTACAAGAA CATGTGCTCC
    GAGGTAATCA AAAAGCATAT ACCACTGAAA TCGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_723214.2
    CDS149..8827
    Translation

    Target ORF information:

    RefSeq Version NM_164709.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster linear ubiquitin E3 ligase, transcript variant E (LUBEL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_164709.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    ATGACGACCC ACCAGTTGCT AAACAAGAAT GTTCGCACCA TGCCCTCCTG GGTGATGGAG 
    GCAAATGACC GCATTGGGCC CAAACCGCCA CCCACGCCAC CAAACGGTGT GGCTGGTGGT
    CTTCCCAAGG CGCCTGCTCT GCCGCCCAAG GCGAAGAGCA CTCCCGAGCC GGACTACGAG
    ATCATCGAAT TCTCCAGCCA GCAGTACTCC AACGAACCGA TGAAGACCAC AGTGATCAGA
    ACCAAGACAC CAGATAACAA ACTAAAGTGC ACCTTGTGCG GTTCCCAGAA TCCCTGGGTA
    ACCTGTGCCG AGTGCGCCGG CCAAATCTTT TGCGCCTCCT GCGACGACAT GTTCCACAAG
    CATCCCAAAC GTAAGCAGCA CATGAGAAAG GCTGTGGAGC AGGGCACACC GCCGATTCCG
    CCAAAGGCAC AGGCCGGTGG TGGGGCACCA CCACCAGTGG CACCTCCACG ACGCAGCAAA
    CGAGGCCTTT TGACACCGTT TCTGGGCCGC AAGGATCAGA TGCTGCCGCC GCCCTCGCCC
    ACGCCCTCGC ACAAGTCGCT GGGCGGCTGG CGGGGATCGC TCGGTGGCGG GGCCACGCCC
    CCAGTGCCAC CAATTGCCAC CAGCTCTGCC AACCAGATGA ACAACCGACC ACTGCCCGAT
    CCACCGCGCA GCGAGGGCGG GGGATCTTCC AGATCGGGCA CCCCCAAGTC CGTGTTCGAC
    ACCATCCAGC GACCGCCGTC GGTGCAGCTG GAGAAGATCA AGAGCAAGGC CAGCGCCACG
    CTGGACCGCA TGGCCATTCT GCAGCAGCGG TACCGCCAAC AGAAGGCTCG CCAGGATCTG
    AGCGCCAACA GCGAACAGCA TTTGTCGAAT GAAGCCGGCT TCGAGCACTG GTCAAACATT
    TCACCATCGC CCTCGCATTT TCGTTCCGGC AGCATGTCAT CCGGCCTGAA CTCATCCCAT
    TTCGATCTCT CGGATGACTC ACAATTTCAC AATTCCTTGC TGCTCCAACA ACGACAGGCG
    GCCGGCGCCC AGCGGCGACA GATGAGCACA TCGGTGTTCA ATCTCAACTC GAATCCACGT
    CGACCACTGT CCGAGGCCCA AAACGGAGGT GCCTGGCTCG CCAATCAGCG CATCCAGCAG
    GCCCAATCCC TGGCGCAGCT AAACTGCGCT GGATGTCAAC AGAGTCAGCA GCATCCTGGC
    TGGGCACAGC ATCCGCACCA AGCACTTCCT CAGCACCAGC ATCCCGATCA GTGGTCACAG
    TTCGGCTCCC AACAGCAATT CAACAACTCC AATCTGTCCC TGAACGTTGG ACCGGGTTAC
    ATGTCGCAGC AACATCATCC ACACTATCCG CCACCAGTGT TTATGACCCA GCGTGGTATG
    ATGCCCAATG TGTATCCAGG AGCACCAGGC TATCCCATGA TGCATCCAGG TGTCATGGGA
    ATGCCACCTT CAGCTGCCTC CAGAGCTGCA TCCCGTTCCC GGTACGCTGC ATCACCAACA
    CCCAGCCGCA AATCGATGTC CTTGCGTCGC AAGCGCAATA GCTACGTGGA TGACGAACTT
    ACGGATGACG AGGACTCCGA CCAGGATGAC CGCAGATCTT TGGTGTCCAA TCGCTCGGGC
    ATGACTAGTG CCTCGCGCTC CCAGCATCAC CAAAACCATA TCCAGCCCCG CCAAAGGCGC
    CTGTCCAGTG CCTCCCAACT CATAGCCAGC GATGAATTGG ACGGCGATCA GGTGCATCAC
    AAGATGCGGA ATCGTCGGGG ATCCATTGCC AAGTCCGTGC AGAGTGAGTG GCTACCGGAG
    CGACGGGAAA ATGAGGGAAC CCTTACCAGA AACAAAACAG CAACGGACTC GGCCAGAACC
    AGTCGCATTT ACTCCGATCT GGAGTCTGAG GGATCGGGTG CTCGGGCCTT GGTTCAAGCT
    AAAATTCAGC AGAAGCTCCA AGAAGCCGAT CAGCACAAAT CCTCGAAGAA GGCTGAGCCA
    AAACGCAAGC CGGAGATGAA GGACGAGAAT ACACAGGCGG CAGCGGTGGT GCAAAAGGTG
    GTGGTGCCAC CAGCCCATGA AGAGAGTGCC TCGGAATACG AGGAAGTGGT CGAAGAGGTA
    ACCGCCTCAG AGAGTGAGGC TGAGGCTCAA ACAGCACCCG ATCCCCAGGA AGTTCCTGAC
    GAGATTGGGG CTGATGATCT CGGCCCACCA CCTTCTACCC CAGACCACGA ATGGGAATGC
    GAGTTCTGCA CGTTTGTGAA CGAGCCCAAC ATCAAGATCT GCTCCATATG CTGCAAGACG
    CCCAGCAAAC CTCCAGTGCA GCCCAACAAG GCCAAAAAGG TAGAGGAAAA ACCACAGCCT
    CCAGCTGAAA AGTCTAACAA TATCAAGGCG TCTTCCAAGA CGGAAACTAA GCCAGTGCAA
    AAGCCTACTA CAAAGAGCCA ACAACCCAGT CAGAAATCCG TTGCTGCTCT TTCGAAGACT
    ACTCACACCA ACTCCACGTC TAGCTCAAAA GCGTCGCCAG CTGTAAACTC CAAGACCACC
    TCCAGTATTC CTATCAAGAC ACCATCGAAA TCCACACTGA AAACTTCGTC GGAAAATGAA
    TCCGATAACT CGCTGGCCAA GAGTCTTCTG CACAAAGAGT CCGTTGAAAA CATTTGGAAT
    ACTCTGGATG AGAGCATACA GGCGCAGGCG GAGCAGGTAC TCAAGAAGGC TCAGAAGGTC
    TCCACGGCCT GTGGTGGGAC TCCTCCCCGG GAAATAGCAG CTGTGGAGAT GGGAACCTCC
    CCGCCTCCAC AAAGCATTTC CACACAGACC TACGACGCTC TGCCCTTCAA TACAAAGCAA
    GAAGAGATCA TCCCCGTTGT TCCTGACCGC TTCACGACTC CAGAACCAAA CAAGATGGAG
    CGACGTCCGC ACTATCGCAG CAATTCCCAG CTGCAACAGG AGAGTGAACG TTATCGGAGT
    GCCAATGACC TGAGGTATCA CGACGGCTTT GGATTGGATC CATATAGTGC CGGTCTGGTC
    ACCAAAAGAC CCAATTTCAT AAACGAGCTA CGCATGCTGC AGCTTCAAGT ATCTTCACCT
    TTCGATATGC CGCATGAGAC ATTCGGAGTC AAGCATGAGC CGGCCAGAGA TCCGGAAACG
    GAAATGCACA TCATACTGAA AGAACTGGAG CTGTACAAGT TTACGGTTGA GGAACTGGAA
    GCTGCCCTTA AGTACTGCTC CCCGGAGACT CATCCGATTC AGTGGCTGCG CGAGAACTGG
    CACAAGCTGG TGCAGACGGT GCAGAGTTTG TCAACCAAGT ACGGACAGGA GAGGGGTGAA
    AACACGATCG GAACTGTGTC CCAAAACGAA GCACGCGAGG CATTGCGCAA CTCGGGGGGA
    AATGTGTGGC AGGCGGTGGC TGACTGCATC CAACAGAGGC AGCAGAAGTA CAGGAAACTG
    GCGGCCAAGG GAAACTTCCT GCGCGATGAC ATTGTCAACG CGCTGACTGC GCACCAAGGT
    AATGTGGAGC AGGCGCTGGT GGAGCTGAAT CGCACGCAAC TCAAGCCATT TCTAATGCGC
    ATCTGGGGCT CGCCGAATGG CGTTGAGAAT GAGAGTGGCG TGGCCATAGA CACCAAGTCG
    GATATCCATG ACTTCTTGAA CACACACGCC TTGGACTGCC TGCAGCCTCC GTTGGCTGGG
    CAAAGTCCCA GCCCCGCCCA AGCCAATCCT TTCGACCAAC CCCGCACCGA CGAGAGCCCC
    GTCAAATCCA CCTATGCCAC ACCCAGTCCC TATCAGATGG AGGACAGCAC CCTGAAGAAC
    CTGGAGATAC TCATTGGCAA CATGGAACAG AACCAGGCCA AGCAGAACCA GGAGGTTCTA
    CGTTCCATCG AGACCATGCT GGACACATTC AAGGGAAAGC CGGAACTGGA GTACGAAACG
    GATCCGGAGA TAATGCGTAT CCTAACAAAG AGCCCCATTA GCACAATGAA ACCATCTGGA
    CCGGCAGAGG ATAAGTCAAC CGATGATGTA AAGAACTTTG TATGGCAACA CATTCAGGAA
    ATTGTGCCCA ACCTGGTGCA ACAGGTTGAA CAGGAGCTCA TGGAGAAACC GGAAGAGGTA
    GCTAAAATTG AGGCAGAACA GCCAAAGGAG CCTGAACCTC TACTCGAACC TCAGCCTGAG
    CCCACACCAA GTGTCGATCC TGCTGTCTAC ATCATGGAGG AAGTCATCAA GCCCAATTTA
    AGGGAAGCCA GCATTCGTGA GGAAGTTCAA CCCAGTTTTA TCTATGCCAC TGAAATAGCC
    AACTTCAAGT TGGAATTTGA TCGTGGCACT GAACGATGGC ACGAGGCGGA ATGGGAAAGC
    AGCGATCTTA CGGATGCAGA ACGCATAGTA TACAAATGTT ATATGGCTCC TAATGAGCAA
    CCCAAGGAGG ATGTTGTAGA TGTAGCGGTT GAAAGTTCAG TCAATTCGCT TCCAACAGCA
    AAAGCACAAG AAGAATCTGC TCCAGAAGTA CCCATTGAAG CTCAAAAGAT TGAAAATACA
    GAAGTTACAC AACCAGAGAC CGTTAATGAG GAACTTCGCC AGCAGGAAAA ACTAGAAACA
    CCATTAGTTA TTACAAGTGA GACAATTAGT GAAACAGTTT CGCAGACAGC CAATGAAAGT
    GATAAACAAA AATCAATTGA AAATAATTTG CAAATTAAGC AGAATGTGGC AGAAGTACAA
    GTTCAGTCGG ATGACCAGCC AAGCACGAGT AGAGATGCCA ACCGAAGAGC TAAGAGATCC
    CAGCAATCGA GAAAGGGTAG ATCCCGCGAG CAAAGCCAAA AACCAACCAA TCGTACAAAG
    CTACCAAACA ATATCGATCA GAAAGTAAAT GAGTCAAAAA CAGCAGCTAA GGAAACAGAA
    GCAGTCAAAG ATAAAGATCT GAGTGCTGCT GCAAGTAATA TTCAGTCAGA TGTTACTGCA
    TCTGATCCTA AAACTAGCAC ACCCCTTAAA ATACTATCTG AAGGAACAAA TAGCAACACT
    TTGGAAACTA TGGAAAACGT AACATCCACA GATATAAACG ATAATGTTAC TATAGAGGTC
    ATCTCAAATC GATCCGAAGA AGTTCCAGCG ATTCAGGATT TGGGAAAAAC GAAGGACATA
    TCTGAACCAA CTGCGAACCC AATCGAAGAA ATTACATCCA TACAAAATAG TACGACTATT
    TCAGAACAAT CTGAAGGGCC TCAAGAACCA GAAATACCTA TCGAAGTATC TGAAACGACA
    GAAGCACTTC AAGTCCCTCG AGAAGAAGCA AGCATTGAAA TTGTCAGCCC TCCAAACGAA
    GAACAAACCA AATCTCCAAC ATCACAGGAA GTTAATATAC AAGACACATC ACACATCATA
    TCTTTACCAA TTACTGATGT TACACCCACT CCAGAAATCA TTAATATAGC GCCTAGTACC
    TCATCAATAT CCAAAGAACA AAAGCAAAGT CCGAAGCGTC TTTCAAAGAT TCCCGTAAGA
    ACCCTGAGCA GCAGCAGTCT TCGCAGTGAG AGTCGGTCGA GCAATCGGAC TCCAACGGCC
    AATGATGAGA TCGAAAGGGA AGAGACCACG TCACAAGGTG TACCCATAGG GGAAACAGTC
    AGTTCTCCAA AATCCGAACA GTTGTCCGAC AACCAGGAGG TTAATCTTGT GAGCCAAGAA
    ACACAGTCCA AAAAGGATAC AAATATTGTC GAAGAACCTG CCACCCAGCC TTTAGGCTTA
    GAGCTGGAAG AACATAGTCC GAATGCAACT GCTGTAGCGG TCAGTCCCAC CGACTCGGAC
    GAAGTATTCG AAGATGCTCC TGAGTTCAGC GGTAGCGATG GAACAAGACC CCATGATGAA
    ACCGCTTCCG ATGCAGAGCT TTATAGCCTT GATAGCGATG GGCAGCGGGC TGAAACCAAG
    TCCCCAGAGG ATGAAGTCGT AATCTTGCTG GATGAGGAAT CCCAGATGGA ATCTTCGATA
    GCCCAGTCCG AAAGCAACGC AAGTCTTGAT TCCCACTCAA GTGAATCCGA GACGTCCAAA
    GTTGTTCTCA AGGAGTTTGT ACCCTCTGGA GATCCGGCCA AACAGAACCT AAGCGAACTG
    GTTGAGGACA CTCAACGTTT GATCAAGCAG ATGCGCGATG AGATCAGCAT GGATGAGTTC
    GAGTCTACCG ATGAGGATGA ATACTCCGAT GAATACTCGG ATGAGTATGA CGAAGGGGAG
    GAGGAGGAGT GGTACGACAG CGAGGGTGAA GAGGAGGGAG ACTTCGATGG GGAGGAGGGT
    AATACCTATA ACGAGCACGC ATCCTACATC GAGGAGGCCT CCACTGGTGA CGAAGGCACC
    GAAATAGAGG ACATAATGGA GGAGGATGAG GATCTGGCTG ATGACGACGA GCCACTGCAA
    TCACAAATTC CACTAGACAT TGAACCAGTC ATATCGCCTG CTCTATCAGT CACGCCCACC
    AACCAAGAAA CTGATACGAT CGCACACACT GAGGTTGTTT CCTCCACAGG AACGAGGCTG
    GAAACAGAGT TGCCGAACCC TGCAATGGAA TCCATCTTGC CTTCTCAAAG TGTTCAGGAG
    GACATCAAGG TGGAAGCGAT TCCTATTCAG TCCGCTCCCC CCATAGCTGA CTCGGAAACT
    CGACCTGCAG AGCAACCAGT TGAGCTTGTG CTAGAAATCC CTTCAGAAGT TGAGCCTACT
    CCCGTTGAAG AGCCCACTGC CCTGCCCATA ACACCTGCAC CACCAATTGT TGATTCCGAA
    TCGCGACCCG TGGAGCCGCC TGTTGAAACA GTACTGGAAG AACCTAAAAA GGTAACCCCG
    AGCATGAAAG GCAAGACTGC AAACAGTGGA ACTGCCTCCA AAGGTCCTTC CACATCAAGT
    TCGACTAAAA CAAACAAGTC CACAGTTAGT AAGATTCCCA AGCCCACCAA TGAACCCACT
    AACAAATCGA ATAGCACCCC CTTGAACAAG AAGGTTCCGC TGCGCTCCAA GTCCTTCTCG
    GCCCCTATGG GCATTTCATC CGTGAAGCGA ATCCAGGAGG TCTACCTCCA GAAGCAGAGT
    TCATCGATTG CAACCAGCCG AGTGCCGCTC AAGAGCAGTC CCGTCACCAA GAAGTCAATC
    AACGATGCCA TCAGCAGGTT TAACTCCAAT CAGGCCGATG GACCCAGCAC CAGTGGAGCA
    GCGGCTGCGG CAGCAGCGGC ATTGTTGAAA CCTCGATCCC AACCCCGAAT CCCAAAGAAG
    AAATACCACG AGACCTGCTT CTCTGACGAT GACTACGAGA CCTCGGCCAC GGAGGAGGAG
    CAGGAGGAAC CCAACTTGGC CGAGCCCCAA AAAGCAGAAC AGCTGAAGCG TAAGATGAGT
    ATGCCCGTTT TTCGGGCATA TCCTAGTGTT CAAGAGCCGG TAATCGAGGA TCCAGCGATC
    CTTGCCCGCA AGTACGTCGA TCAGGAGCTG GTGACCAATA TCGCAGAGGC CCAGATAGCC
    GCCACCTTGG TGAGCATGAA GTTCTCGGAA GACGTGGCTC TGTGGGCGGC CAGGGAGTGC
    TCCGATCTGG ACCAGGCCAT TGCCATGCTC CAGCAGGAGT GCGAGCTCTG CATGAACAGC
    TATCCCATGA ATCAGATGGT GTCCATGCTA AAGTGTCTGC ACAAGTGCTG CAAGCAGTGT
    GCCAAGAGCT ACTTTACAGT ACAGATAACC GATCGCAGTA TAAACGATTG CAGTTGCCCG
    TTTTGCAAGC TGCCCGAGTT GAGCAACGAG GCTCAGCATG AGGATGAGCA CCTCGAGTAC
    TTCTCCAACC TGGACATCTT CCTGAAGAGC ATCCTGGACA ACGATGTGCA CGAACTGTTC
    CAGCGCAAGT TGCGCGATCG CTCGCTGCTG CAGGATCCCA ACTTCAAGTG GTGCATCCAG
    TGCTCGTCCG GATTCTTCGC TCGGCCCAAG CAGAAGCGAT TGATTTGTCC GGACTGTGGA
    TCCGTTACCT GTGCCCAGTG CCGAAAGCCC TGGGAGCGCC AGCACGAGGG CAGTAGCTGC
    GAAGCTTATC TGGAATGGAA GCGCGAAAAC GATCCCGAGC TGCAGGCCCA GGGTGTCCAG
    GAGCATTTGG CCCAAAACGG CATCGACTGT CCCAAGTGCA AGTTCCGATA CTCGCTGGCC
    AGAGGCGGAT GCATGCATTT TACCTGTACT CAGTGCAAAT TCGAGTTCTG CTACGGATGT
    GCCCGACCCT TCATGATGGG TGCCAAGTGC ACTGTGTCCA CATACTGTGC CAAGCTCGGT
    CTCCATGCCC ATCATCCGCG CAACTGCCTG TTCTACCTGA GGGATAAAAT CCCCCTTCAG
    TTGCAGTTCC TGCTCAAGGA GCAAAACGTC AAATTCGACA CGGAACCCAT GCAGATCAAG
    GACGAGTCCA GCAGTTCGTC CAAGGCTCGT GCCCAGGCCC GCTGCCCGAT CCCGCTGCAA
    AAGGAGACGC CGCAAGGACT AGTCGACACA GTGTGCAACA CGGAAGTTCC TGACAAGCAC
    GCTGGCATGT GTCGCACCCA CTACGTAGAG TATTTGGCTG GCAAGGTTGC CAAGGCTGGC
    ATCGATCCAC TGCCCATCTT TGATCTGACG GACTGCGTCC AGGAGCTGCG AAGGCGAGGC
    ATCGCTCTGC CGGAGAGAGG ACCCTGGGAC ACCGATGAGA TCTACAAGAA CATGTGCTCC
    GAGGTAATCA AAAAGCATAT ACCACTGAAA TCGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.