retm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	retm
Entrez Gene ID	33899
Full Name	real-time
Synonyms	CG9528,Dmel\CG9528
General protein information	Preferred Names real-time Names CG9528 gene product from transcript CG9528-RC CG9528-PA CG9528-PB CG9528-PC retm-PA retm-PB retm-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 26D4-26D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273203.2	NP_001260132.1	real-time, isoform B
NM_135184.4	NP_609028.2	real-time, isoform A
NM_001298743.1	NP_001285672.1	real-time, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SEC14L5	XP_414710.3
Bos taurus (cattle)	SEC14L5	NP_001178188.1
Mus musculus (house mouse)	Sec14l5	NP_001121197.1
Drosophila melanogaster (fruit fly)	retm	NP_609028.2
Anopheles gambiae (African malaria mosquito)	RETM_ANOGA	XP_319779.4
Xenopus tropicalis (tropical clawed frog)	sec14l1	NP_001096225.1
Homo sapiens (human)	SEC14L5	NP_055507.1
Saccharomyces cerevisiae (baker's yeast)	YKL091C	NP_012832.1
Pan troglodytes (chimpanzee)	SEC14L5	XP_003314986.1
Canis lupus familiaris (dog)	SEC14L5	XP_005621638.1
Rattus norvegicus (Norway rat)	Sec14l5	NP_001129182.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we provide evidence that, like Slmo, both the Prel and Retm proteins are localised to the mitochondria, indicating that the function of the PRELI/MSF1p' domain is specific to this organelle.
Title: A novel family of mitochondrial proteins is represented by the Drosophila genes slmo, preli-like and real-time.

The following retm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the retm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13910	NM_135184.4 Latest version!	Drosophila melanogaster real-time (retm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13910	NM_001298743.1 Latest version!	Drosophila melanogaster real-time (retm), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15804	NM_001273203.2 Latest version!	Drosophila melanogaster real-time (retm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

retm ( NM_135184.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609028.2 Drosophila melanogaster real-time (retm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13910
Clone ID Related Accession (Same CDS sequence)	NM_135184.4 , NM_001298743.1
Accession Version	NM_135184.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	real-time, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135184.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609028.2
CDS	333..2312
Translation	MVQKFQSPVRVYKYPFELVMKAYERRFPTCPQMPIVLDCEVIKDESLEDGAKRNTSRRCKLAVDAPYIFKKLIGVDHVYFLQHNFLDLANRTLSIEAVNESFSSRIEIFERCRYYAHPDNSEWTCFDQSATLDIKNFFGFEHSMEKMGMKQYTQTTLKGKEIIEFFIGQLREEGITHVERWTSPSDATKSPTLDQASDQQHSILLDGDFIARSLGQLSPMQESKLLELRKMLDGVDDLERVPSYQTILRFLAARDWHVSQAYAMLCDSLRWRREHRIDALLAEYSKPAVVVEHFPGGWHHLDKDGRPVYILRLGHMDVKGLLKSLGMDGLLRLALHICEEGIQKINESAERLEKPVLNWSLLVDLEGLSMRHLWRPGIKALLNIIETVERNYPETMGRVLVVRAPRVFPIAWTIVSAFIDEHTRSKFLFYGPDCAHMKDGLAQYLDEEIVPDFLGGPCKTMIHEGGLVPKTLYKMNSLEDHDDEVTAELPTTAAAQALVPGKRLSANQQHDHRNLYKSVDLKAGFAHELLIRNEDPKSVLTWDFDVMRNDLHFTLYRVTQELPEKNDSAVSYFDLQDFVEGVNYFREEPTLICRHKESVQGSHVMHHNDSYLMHWFSPSGAQLNVFYEVLSSANYKGSMTSLQSAFSSNSSAASSVQSR

Target ORF information:

RefSeq Version	NM_135184.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster real-time (retm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135184.4

ORF Insert Sequence:

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

retm ( NM_001298743.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285672.1 Drosophila melanogaster real-time (retm), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13910
Clone ID Related Accession (Same CDS sequence)	NM_135184.4 , NM_001298743.1
Accession Version	NM_001298743.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	real-time, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285672.1
CDS	333..2312
Translation	MVQKFQSPVRVYKYPFELVMKAYERRFPTCPQMPIVLDCEVIKDESLEDGAKRNTSRRCKLAVDAPYIFKKLIGVDHVYFLQHNFLDLANRTLSIEAVNESFSSRIEIFERCRYYAHPDNSEWTCFDQSATLDIKNFFGFEHSMEKMGMKQYTQTTLKGKEIIEFFIGQLREEGITHVERWTSPSDATKSPTLDQASDQQHSILLDGDFIARSLGQLSPMQESKLLELRKMLDGVDDLERVPSYQTILRFLAARDWHVSQAYAMLCDSLRWRREHRIDALLAEYSKPAVVVEHFPGGWHHLDKDGRPVYILRLGHMDVKGLLKSLGMDGLLRLALHICEEGIQKINESAERLEKPVLNWSLLVDLEGLSMRHLWRPGIKALLNIIETVERNYPETMGRVLVVRAPRVFPIAWTIVSAFIDEHTRSKFLFYGPDCAHMKDGLAQYLDEEIVPDFLGGPCKTMIHEGGLVPKTLYKMNSLEDHDDEVTAELPTTAAAQALVPGKRLSANQQHDHRNLYKSVDLKAGFAHELLIRNEDPKSVLTWDFDVMRNDLHFTLYRVTQELPEKNDSAVSYFDLQDFVEGVNYFREEPTLICRHKESVQGSHVMHHNDSYLMHWFSPSGAQLNVFYEVLSSANYKGSMTSLQSAFSSNSSAASSVQSR

Target ORF information:

RefSeq Version	NM_001298743.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster real-time (retm), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298743.1

ORF Insert Sequence:

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

retm ( NM_001273203.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260132.1 Drosophila melanogaster real-time (retm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15804
Clone ID Related Accession (Same CDS sequence)	NM_001273203.2
Accession Version	NM_001273203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	real-time, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273203.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA 
TATGAGAAAG CCGGGGATGG CGGAACGAAC GGCGCCGAAT GGAGATCCCC GGCATTGGAG 
GAGCCCATAG CGGCGGACAT TCTCACGGGC ACCCTGATGG AGAAGAGCCT GAATCTATTT 
GAAACGCCGG ACGTATTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260132.1
CDS	333..2456
Translation	MVQKFQSPVRVYKYPFELVMKAYERRFPTCPQMPIVLDCEVIKDESLEDGAKRNTSRRCKLAVDAPYIFKKLIGVDHVYFLQHNFLDLANRTLSIEAVNESFSSRIEIFERCRYYAHPDNSEWTCFDQSATLDIKNFFGFEHSMEKMGMKQYTQTTLKGKEIIEFFIGQLREEGITHVERWTSPSDATKSPTLDQASDQQHSILLDGDFIARSLGQLSPMQESKLLELRKMLDGVDDLERVPSYQTILRFLAARDWHVSQAYAMLCDSLRWRREHRIDALLAEYSKPAVVVEHFPGGWHHLDKDGRPVYILRLGHMDVKGLLKSLGMDGLLRLALHICEEGIQKINESAERLEKPVLNWSLLVDLEGLSMRHLWRPGIKALLNIIETVERNYPETMGRVLVVRAPRVFPIAWTIVSAFIDEHTRSKFLFYGPDCAHMKDGLAQYLDEEIVPDFLGGPCKTMIHEGGLVPKTLYKMNSLEDHDDEVTAELPTTAAAQALVPGKRLSANQQHDHRNLYKSVDLKAGFAHELLIRNEDPKSVLTWDFDVMRNDLHFTLYRVTQELPEKNDSAVSYFDLQDFVEGVNYFREEPTLICRHKESVQGSHVMHHNDSYLMHWFSPSGAQLNVFYEVLSSANYKGSMTSLQSAFSSNSSAASSVQSR*YEKAGDGGTNGAEWRSPALEEPIAADILTGTLMEKSLNLFETPDVFF

Target ORF information:

RefSeq Version	NM_001273203.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster real-time (retm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273203.2

ORF Insert Sequence:

ATGGTGCAAA AATTCCAGTC ACCCGTTCGC GTCTACAAAT ATCCTTTTGA GCTGGTGATG 
AAGGCCTATG AGCGGCGGTT TCCCACATGC CCGCAGATGC CCATCGTCCT GGACTGTGAG 
GTCATTAAGG ACGAGTCCCT GGAGGACGGG GCCAAGCGAA ACACGAGCCG ACGCTGCAAG 
CTGGCGGTCG ACGCGCCGTA CATCTTCAAA AAGCTTATTG GCGTGGACCA TGTGTACTTC 
CTGCAGCACA ACTTCCTGGA CCTGGCCAAC CGCACGCTGA GCATCGAGGC GGTCAACGAG 
AGCTTCTCGT CGCGCATCGA AATCTTCGAG CGGTGTCGCT ACTACGCCCA TCCGGACAAC 
TCCGAATGGA CGTGCTTTGA CCAGTCGGCC ACGCTGGACA TTAAGAACTT CTTCGGCTTC 
GAGCACTCGA TGGAAAAGAT GGGCATGAAG CAGTACACCC AGACGACGCT GAAGGGCAAA 
GAAATCATTG AGTTCTTTAT CGGGCAGCTG CGCGAGGAAG GCATCACGCA TGTGGAGCGT 
TGGACATCGC CCAGTGATGC AACCAAATCG CCAACCCTCG ATCAGGCTTC GGATCAGCAG 
CATAGCATCT TGCTGGACGG CGACTTTATC GCACGCAGCC TGGGCCAGCT GTCGCCCATG 
CAGGAGTCCA AGCTGCTGGA GCTGCGAAAA ATGCTGGATG GTGTCGATGA CTTGGAGCGT 
GTGCCCAGTT ATCAGACCAT CCTGCGTTTC TTAGCAGCCA GGGACTGGCA CGTTAGCCAG 
GCCTACGCCA TGCTCTGCGA CTCGCTTCGC TGGCGTCGGG AACATCGCAT CGACGCACTG 
TTGGCGGAGT ACTCCAAGCC AGCCGTGGTA GTGGAACACT TTCCGGGCGG CTGGCATCAT 
TTGGACAAGG ACGGTCGACC CGTTTACATT CTGCGCCTGG GACACATGGA CGTCAAGGGC 
CTGCTAAAGT CACTGGGAAT GGATGGACTA CTGCGATTGG CTTTGCACAT CTGCGAGGAA 
GGCATTCAGA AGATCAATGA ATCCGCCGAA CGCCTGGAGA AGCCAGTTCT CAACTGGTCA 
CTGCTTGTCG ATCTGGAGGG TCTTTCTATG CGCCACCTCT GGCGTCCCGG CATCAAAGCC 
CTGCTCAACA TCATCGAGAC TGTGGAGCGC AATTACCCAG AGACCATGGG CCGTGTCCTA 
GTGGTGCGAG CGCCCAGAGT GTTTCCCATT GCATGGACAA TCGTTAGTGC TTTTATTGAT 
GAGCACACCC GATCCAAGTT CCTGTTCTAC GGCCCCGACT GTGCGCACAT GAAGGATGGC 
CTGGCCCAGT ATCTCGACGA GGAGATTGTG CCTGACTTCC TGGGCGGACC GTGCAAGACC 
ATGATTCACG AGGGCGGACT GGTGCCTAAG ACGTTGTACA AGATGAATTC GCTGGAGGAT 
CACGACGACG AAGTGACCGC CGAGCTGCCC ACCACTGCCG CGGCACAGGC TTTGGTTCCA 
GGCAAGCGCC TGTCTGCCAA CCAGCAACAC GATCATCGCA ATCTGTACAA GAGTGTGGAT 
CTCAAGGCCG GATTCGCGCA CGAGCTGCTC ATCCGGAACG AGGATCCCAA GAGTGTGCTC 
ACCTGGGACT TCGATGTGAT GCGCAACGAT CTGCACTTTA CGCTCTACCG GGTCACCCAG 
GAGCTTCCGG AAAAGAATGA TTCCGCCGTC TCGTACTTTG ATCTGCAGGA CTTCGTCGAG 
GGCGTCAATT ATTTCCGTGA GGAGCCAACG CTGATTTGCC GGCACAAGGA GAGCGTCCAG 
GGGTCGCACG TGATGCATCA CAACGACAGC TATCTTATGC ACTGGTTTAG TCCATCGGGT 
GCCCAGTTAA ACGTCTTCTA CGAGGTGCTC AGCTCGGCCA ACTACAAGGG TTCGATGACC 
AGCCTGCAGT CGGCATTCTC ATCGAACTCG TCGGCGGCCA GCTCGGTGCA GAGTCGATGA 
TATGAGAAAG CCGGGGATGG CGGAACGAAC GGCGCCGAAT GGAGATCCCC GGCATTGGAG 
GAGCCCATAG CGGCGGACAT TCTCACGGGC ACCCTGATGG AGAAGAGCCT GAATCTATTT 
GAAACGCCGG ACGTATTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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retm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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