ine cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ine
Entrez Gene ID	33659
Full Name	inebriated
Synonyms	CG15444,CG15444d,DMINE,Dmel\CG15444,ine/rosA,rosA
General protein information	Preferred Names inebriated Names CG15444 gene product from transcript CG15444-RC CG15444-PA CG15444-PB CG15444-PC CG15444-PE CG15444-PF ine-PA ine-PB ine-PC ine-PE ine-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24F4-24F4\|2-12 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057664.6	NP_477012.1	inebriated, isoform B
NM_001169400.2	NP_001162871.1	inebriated, isoform F
NM_057665.5	NP_477013.1	inebriated, isoform A
NM_175959.3	NP_787973.1	inebriated, isoform E
NM_175958.3	NP_787972.1	inebriated, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008940	XP_319694.4
Magnaporthe oryzae (rice blast fungus)	MGG_12737	XP_003716693.1
Danio rerio (zebrafish)	LOC569007	XP_001921114.2
Danio rerio (zebrafish)	si:ch211-225b11.1	NP_001103931.1
Magnaporthe oryzae (rice blast fungus)	MGG_02075	XP_003708841.1
Magnaporthe oryzae (rice blast fungus)	MGG_05433	XP_003710293.1
Drosophila melanogaster (fruit fly)	ine	NP_477013.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

two protein isoforms encoded by the inebriated (ine) gene perform osmolyte transport within the Malpighian tubule and hindgut
Title: The Drosophila inebriated-encoded neurotransmitter/osmolyte transporter: dual roles in the control of neuronal excitability and the osmotic stress response.

The following ine gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ine cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13743	NM_057665.5 Latest version!	Drosophila melanogaster inebriated (ine), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13742	NM_001169400.2 Latest version!	Drosophila melanogaster inebriated (ine), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13742	NM_057664.6 Latest version!	Drosophila melanogaster inebriated (ine), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13742	NM_175959.3 Latest version!	Drosophila melanogaster inebriated (ine), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13742	NM_175958.3 Latest version!	Drosophila melanogaster inebriated (ine), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ine ( NM_057665.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477013.1 Drosophila melanogaster inebriated (ine), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13743
Clone ID Related Accession (Same CDS sequence)	NM_057665.5
Accession Version	NM_057665.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inebriated, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057665.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAACC GCCAGGACTA CGATGCACAG TCCTCGAAGC ACTCGGAACA GTCCTTCTTC 
CGCTTCAACC GGGTGGCCAA AGTACCCCTG GGGGTGGATG ACGATGACAG CTACCTGGAC 
ATGTCCGATG AGACGGCCCA GCTCAAGCCG CGCCAGCAGC ACTGGGCCAA TAAGATGCAA 
TTCGTCCTGG CCTGCATAGG ATACTCGGTG GGTCTGGGCA ACGTCTGGCG TTTTCCGTAC 
ATGTGCTACA AGAGTGGCGG TGGTGTTTTT CTAGTACCTT ATTGCATAAT ACTGTTCATA 
TGCAGCATTC CGCTGCTCTT CATGGAGCTG TCCGTTGGCC AATACACCGG TCGAGGACCC 
ATTGGAGCAC TGGGTCAACT GTGTCCTCTG TTCAAGGGAG CCGGACTCGC CAGCGTGGTC 
GTCTCGTTCC TCATGTCCAC CTACTATAGT GTCATCATAG GCTACTCCAT CTACTACTTC 
TTTACGTCGT TCAAAACGGA AATGCCCTGG ATCGACTGCA ACAACAGGTG GAACACGCCC 
GATTGCTGGG TGCCACAGCG CAAGGGGATT AATGCCAGTG CGCCGGACAC ATCTCGCACT 
CCATCCGAGG AATTCTTCGA AAACAAGGTC CTGCAGATCA GCGGTGGCCT GGAGTATCCG 
GGCATGATGC GCTGGGAGCT ATTTGCCTGC CTGATCTGCG CCTGGCTAAT GGTCTACTTC 
GCCACGTGGA AGTCGATCAA GTCGTCGGCG AAGGTGCGCT ACTTCACGGC CACGTTTCCG 
TTCGTGCTGA TCATCATCCT GATGGTGCGC GCGGTGACCC TGGATGGAGC TGCTGAGGGT 
TTGCGCTTCT TCTTCCGTCC CAAGTGGTCG GAGCTGAAGA ACGCCAATGT GTGGATCAAC 
GCCGCATCCC AGAACTTCAA CTCGCTGGGC ATTACATTCG GATCCATGAT CTCCTTTGCC 
AGCTACAACA AGTACAACAA CAATATCCTG CGAGATACCG TGGCAGTGAG TGCGGTGAAT 
ATGATCACCA GTCTCCTGGT GGGCATCTTT GCCTTCTCCA CGCTGGGCAA TCTGGCGCTG 
GAGCAGAACA CCAATGTGAG GGACGTCATC GGTGATGGAC CGGGCATGAT ATTCGTGGTG 
TATCCCCAGG CGATGGCTAA GATGCCGTAC GCTCAGCTCT GGGCGGTCAT GTTCTTCTTC 
ATGCTGCTCT GCCTAGGTCT GAACTCGCAG TTTGCCATTG TGGAGGTTGT GGTCACGTCC 
ATACAGGATG GCTTTCCGCG GTGGATCAAG CGGCACCTGG GCTATCACGA AATCGTGGTC 
CTGTTCGTCT GTGTCATCTC ATGTCTCTTC GGGATGCCCA ACATCATACA GGGTGGCATC 
TACTACTTCC AGCTAATGGA TCACTACGCC GCCTCCGTGA CGATCATGTT TCTGGCCTTC 
TGTCAAATGA TTGCCATTGC CTGGTTCTAC GGCACGGGAA GACTGTCGAA GAACGTCAAG 
CAAATGACCG GAAAGGCGCC TTCGTTCTAT CTCAGATCCT GCTGGCTAGT CCTGGGACCC 
TGCCTCCTCT TTGCCATCTG GGTATTGAGT CTGATCAACT ACCACGAGCC CACCTACCAC 
AATGGACGCT ATACCTATCC GGACTGGGCC TACGGAATAG GCTGGATGTT CGCTTCATTC 
TCGCTGATCT GCATACCTGG ATATGCGGTT ATCAACTTCC TGCGCTCCAG TGGAGATACC 
TTTTGGGAAC GCATCCGGAA CACGCTGAGA CCCAACATCT ACGAGTGCAA GATTTGCGGG 
GAACACCACT GCGAACACGA CTATCCGGAG CAGGAGCAGT TTATGCTGGC CCAGGAAATG 
GCCACAGTGT ACAAGCCCAC CAACCCACAC CTGCTCAATC TCGGCCAGAA GTGCGGCTAC 
AACGCCATGC AAGCCTCACC CTCACATGCG GAGGCAGGAG GACCATGTGG CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477013.1
CDS	162..2138
Translation	MPNRQDYDAQSSKHSEQSFFRFNRVAKVPLGVDDDDSYLDMSDETAQLKPRQQHWANKMQFVLACIGYSVGLGNVWRFPYMCYKSGGGVFLVPYCIILFICSIPLLFMELSVGQYTGRGPIGALGQLCPLFKGAGLASVVVSFLMSTYYSVIIGYSIYYFFTSFKTEMPWIDCNNRWNTPDCWVPQRKGINASAPDTSRTPSEEFFENKVLQISGGLEYPGMMRWELFACLICAWLMVYFATWKSIKSSAKVRYFTATFPFVLIIILMVRAVTLDGAAEGLRFFFRPKWSELKNANVWINAASQNFNSLGITFGSMISFASYNKYNNNILRDTVAVSAVNMITSLLVGIFAFSTLGNLALEQNTNVRDVIGDGPGMIFVVYPQAMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRHLGYHEIVVLFVCVISCLFGMPNIIQGGIYYFQLMDHYAASVTIMFLAFCQMIAIAWFYGTGRLSKNVKQMTGKAPSFYLRSCWLVLGPCLLFAIWVLSLINYHEPTYHNGRYTYPDWAYGIGWMFASFSLICIPGYAVINFLRSSGDTFWERIRNTLRPNIYECKICGEHHCEHDYPEQEQFMLAQEMATVYKPTNPHLLNLGQKCGYNAMQASPSHAEAGGPCGQ

Target ORF information:

RefSeq Version	NM_057665.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inebriated (ine), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057665.5

ORF Insert Sequence:

ATGCCGAACC GCCAGGACTA CGATGCACAG TCCTCGAAGC ACTCGGAACA GTCCTTCTTC 
CGCTTCAACC GGGTGGCCAA AGTACCCCTG GGGGTGGATG ACGATGACAG CTACCTGGAC 
ATGTCCGATG AGACGGCCCA GCTCAAGCCG CGCCAGCAGC ACTGGGCCAA TAAGATGCAA 
TTCGTCCTGG CCTGCATAGG ATACTCGGTG GGTCTGGGCA ACGTCTGGCG TTTTCCGTAC 
ATGTGCTACA AGAGTGGCGG TGGTGTTTTT CTAGTACCTT ATTGCATAAT ACTGTTCATA 
TGCAGCATTC CGCTGCTCTT CATGGAGCTG TCCGTTGGCC AATACACCGG TCGAGGACCC 
ATTGGAGCAC TGGGTCAACT GTGTCCTCTG TTCAAGGGAG CCGGACTCGC CAGCGTGGTC 
GTCTCGTTCC TCATGTCCAC CTACTATAGT GTCATCATAG GCTACTCCAT CTACTACTTC 
TTTACGTCGT TCAAAACGGA AATGCCCTGG ATCGACTGCA ACAACAGGTG GAACACGCCC 
GATTGCTGGG TGCCACAGCG CAAGGGGATT AATGCCAGTG CGCCGGACAC ATCTCGCACT 
CCATCCGAGG AATTCTTCGA AAACAAGGTC CTGCAGATCA GCGGTGGCCT GGAGTATCCG 
GGCATGATGC GCTGGGAGCT ATTTGCCTGC CTGATCTGCG CCTGGCTAAT GGTCTACTTC 
GCCACGTGGA AGTCGATCAA GTCGTCGGCG AAGGTGCGCT ACTTCACGGC CACGTTTCCG 
TTCGTGCTGA TCATCATCCT GATGGTGCGC GCGGTGACCC TGGATGGAGC TGCTGAGGGT 
TTGCGCTTCT TCTTCCGTCC CAAGTGGTCG GAGCTGAAGA ACGCCAATGT GTGGATCAAC 
GCCGCATCCC AGAACTTCAA CTCGCTGGGC ATTACATTCG GATCCATGAT CTCCTTTGCC 
AGCTACAACA AGTACAACAA CAATATCCTG CGAGATACCG TGGCAGTGAG TGCGGTGAAT 
ATGATCACCA GTCTCCTGGT GGGCATCTTT GCCTTCTCCA CGCTGGGCAA TCTGGCGCTG 
GAGCAGAACA CCAATGTGAG GGACGTCATC GGTGATGGAC CGGGCATGAT ATTCGTGGTG 
TATCCCCAGG CGATGGCTAA GATGCCGTAC GCTCAGCTCT GGGCGGTCAT GTTCTTCTTC 
ATGCTGCTCT GCCTAGGTCT GAACTCGCAG TTTGCCATTG TGGAGGTTGT GGTCACGTCC 
ATACAGGATG GCTTTCCGCG GTGGATCAAG CGGCACCTGG GCTATCACGA AATCGTGGTC 
CTGTTCGTCT GTGTCATCTC ATGTCTCTTC GGGATGCCCA ACATCATACA GGGTGGCATC 
TACTACTTCC AGCTAATGGA TCACTACGCC GCCTCCGTGA CGATCATGTT TCTGGCCTTC 
TGTCAAATGA TTGCCATTGC CTGGTTCTAC GGCACGGGAA GACTGTCGAA GAACGTCAAG 
CAAATGACCG GAAAGGCGCC TTCGTTCTAT CTCAGATCCT GCTGGCTAGT CCTGGGACCC 
TGCCTCCTCT TTGCCATCTG GGTATTGAGT CTGATCAACT ACCACGAGCC CACCTACCAC 
AATGGACGCT ATACCTATCC GGACTGGGCC TACGGAATAG GCTGGATGTT CGCTTCATTC 
TCGCTGATCT GCATACCTGG ATATGCGGTT ATCAACTTCC TGCGCTCCAG TGGAGATACC 
TTTTGGGAAC GCATCCGGAA CACGCTGAGA CCCAACATCT ACGAGTGCAA GATTTGCGGG 
GAACACCACT GCGAACACGA CTATCCGGAG CAGGAGCAGT TTATGCTGGC CCAGGAAATG 
GCCACAGTGT ACAAGCCCAC CAACCCACAC CTGCTCAATC TCGGCCAGAA GTGCGGCTAC 
AACGCCATGC AAGCCTCACC CTCACATGCG GAGGCAGGAG GACCATGTGG CCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ine ( NM_057664.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477012.1 Drosophila melanogaster inebriated (ine), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13742
Clone ID Related Accession (Same CDS sequence)	NM_057664.6 , NM_001169400.2 , NM_175959.3 , NM_175958.3
Accession Version	NM_057664.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inebriated, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057664.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477012.1
CDS	699..3530
Translation	MAENKDVSQVLNTTPDQVVINRAPPTGLAPLRHSQLSDSGAESCYEGDEQARLIRSSSSRAHKFIIIPATPGTPPGSGAVAGPLPFRQTSSTTSLAAMAAALHKQSPLRHTSVRTRPSSEVLQPGQVLANQPTAAVSTSTVTFTIDDCNEEGDAIPAAASVPAPVTMPAISPTPATLTCTTTTTMAGDSVAVSPLSMELKSRLGSHHSSMRSVVSGYLPGLSDSSGNLVGGVSMATSGLQAPNPLYMQPQASLSGSSYHFHELAGNQIYSDVTSVRSLASIGIGSTDGRKLVIRRVPTTANELFDMVNPQTPPPLGVDDDDSYLDMSDETAQLKPRQQHWANKMQFVLACIGYSVGLGNVWRFPYMCYKSGGGVFLVPYCIILFICSIPLLFMELSVGQYTGRGPIGALGQLCPLFKGAGLASVVVSFLMSTYYSVIIGYSIYYFFTSFKTEMPWIDCNNRWNTPDCWVPQRKGINASAPDTSRTPSEEFFENKVLQISGGLEYPGMMRWELFACLICAWLMVYFATWKSIKSSAKVRYFTATFPFVLIIILMVRAVTLDGAAEGLRFFFRPKWSELKNANVWINAASQNFNSLGITFGSMISFASYNKYNNNILRDTVAVSAVNMITSLLVGIFAFSTLGNLALEQNTNVRDVIGDGPGMIFVVYPQAMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRHLGYHEIVVLFVCVISCLFGMPNIIQGGIYYFQLMDHYAASVTIMFLAFCQMIAIAWFYGTGRLSKNVKQMTGKAPSFYLRSCWLVLGPCLLFAIWVLSLINYHEPTYHNGRYTYPDWAYGIGWMFASFSLICIPGYAVINFLRSSGDTFWERIRNTLRPNIYECKICGEHHCEHDYPEQEQFMLAQEMATVYKPTNPHLLNLGQKCGYNAMQASPSHAEAGGPCGQ

Target ORF information:

RefSeq Version	NM_057664.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inebriated (ine), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057664.6

ORF Insert Sequence:

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ine ( NM_001169400.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162871.1 Drosophila melanogaster inebriated (ine), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13742
Clone ID Related Accession (Same CDS sequence)	NM_057664.6 , NM_001169400.2 , NM_175959.3 , NM_175958.3
Accession Version	NM_001169400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inebriated, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001169400.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162871.1
CDS	501..3332
Translation	MAENKDVSQVLNTTPDQVVINRAPPTGLAPLRHSQLSDSGAESCYEGDEQARLIRSSSSRAHKFIIIPATPGTPPGSGAVAGPLPFRQTSSTTSLAAMAAALHKQSPLRHTSVRTRPSSEVLQPGQVLANQPTAAVSTSTVTFTIDDCNEEGDAIPAAASVPAPVTMPAISPTPATLTCTTTTTMAGDSVAVSPLSMELKSRLGSHHSSMRSVVSGYLPGLSDSSGNLVGGVSMATSGLQAPNPLYMQPQASLSGSSYHFHELAGNQIYSDVTSVRSLASIGIGSTDGRKLVIRRVPTTANELFDMVNPQTPPPLGVDDDDSYLDMSDETAQLKPRQQHWANKMQFVLACIGYSVGLGNVWRFPYMCYKSGGGVFLVPYCIILFICSIPLLFMELSVGQYTGRGPIGALGQLCPLFKGAGLASVVVSFLMSTYYSVIIGYSIYYFFTSFKTEMPWIDCNNRWNTPDCWVPQRKGINASAPDTSRTPSEEFFENKVLQISGGLEYPGMMRWELFACLICAWLMVYFATWKSIKSSAKVRYFTATFPFVLIIILMVRAVTLDGAAEGLRFFFRPKWSELKNANVWINAASQNFNSLGITFGSMISFASYNKYNNNILRDTVAVSAVNMITSLLVGIFAFSTLGNLALEQNTNVRDVIGDGPGMIFVVYPQAMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRHLGYHEIVVLFVCVISCLFGMPNIIQGGIYYFQLMDHYAASVTIMFLAFCQMIAIAWFYGTGRLSKNVKQMTGKAPSFYLRSCWLVLGPCLLFAIWVLSLINYHEPTYHNGRYTYPDWAYGIGWMFASFSLICIPGYAVINFLRSSGDTFWERIRNTLRPNIYECKICGEHHCEHDYPEQEQFMLAQEMATVYKPTNPHLLNLGQKCGYNAMQASPSHAEAGGPCGQ

Target ORF information:

RefSeq Version	NM_001169400.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inebriated (ine), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169400.2

ORF Insert Sequence:

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ine ( NM_175959.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787973.1 Drosophila melanogaster inebriated (ine), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa13742
Clone ID Related Accession (Same CDS sequence)	NM_057664.6 , NM_001169400.2 , NM_175959.3 , NM_175958.3
Accession Version	NM_175959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inebriated, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_175959.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787973.1
CDS	523..3354
Translation	MAENKDVSQVLNTTPDQVVINRAPPTGLAPLRHSQLSDSGAESCYEGDEQARLIRSSSSRAHKFIIIPATPGTPPGSGAVAGPLPFRQTSSTTSLAAMAAALHKQSPLRHTSVRTRPSSEVLQPGQVLANQPTAAVSTSTVTFTIDDCNEEGDAIPAAASVPAPVTMPAISPTPATLTCTTTTTMAGDSVAVSPLSMELKSRLGSHHSSMRSVVSGYLPGLSDSSGNLVGGVSMATSGLQAPNPLYMQPQASLSGSSYHFHELAGNQIYSDVTSVRSLASIGIGSTDGRKLVIRRVPTTANELFDMVNPQTPPPLGVDDDDSYLDMSDETAQLKPRQQHWANKMQFVLACIGYSVGLGNVWRFPYMCYKSGGGVFLVPYCIILFICSIPLLFMELSVGQYTGRGPIGALGQLCPLFKGAGLASVVVSFLMSTYYSVIIGYSIYYFFTSFKTEMPWIDCNNRWNTPDCWVPQRKGINASAPDTSRTPSEEFFENKVLQISGGLEYPGMMRWELFACLICAWLMVYFATWKSIKSSAKVRYFTATFPFVLIIILMVRAVTLDGAAEGLRFFFRPKWSELKNANVWINAASQNFNSLGITFGSMISFASYNKYNNNILRDTVAVSAVNMITSLLVGIFAFSTLGNLALEQNTNVRDVIGDGPGMIFVVYPQAMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRHLGYHEIVVLFVCVISCLFGMPNIIQGGIYYFQLMDHYAASVTIMFLAFCQMIAIAWFYGTGRLSKNVKQMTGKAPSFYLRSCWLVLGPCLLFAIWVLSLINYHEPTYHNGRYTYPDWAYGIGWMFASFSLICIPGYAVINFLRSSGDTFWERIRNTLRPNIYECKICGEHHCEHDYPEQEQFMLAQEMATVYKPTNPHLLNLGQKCGYNAMQASPSHAEAGGPCGQ

Target ORF information:

RefSeq Version	NM_175959.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inebriated (ine), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175959.3

ORF Insert Sequence:

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ine ( NM_175958.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787972.1 Drosophila melanogaster inebriated (ine), transcript variant C, mRNA.

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CloneID	OFa13742
Clone ID Related Accession (Same CDS sequence)	NM_057664.6 , NM_001169400.2 , NM_175959.3 , NM_175958.3
Accession Version	NM_175958.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inebriated, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_175958.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787972.1
CDS	487..3318
Translation	MAENKDVSQVLNTTPDQVVINRAPPTGLAPLRHSQLSDSGAESCYEGDEQARLIRSSSSRAHKFIIIPATPGTPPGSGAVAGPLPFRQTSSTTSLAAMAAALHKQSPLRHTSVRTRPSSEVLQPGQVLANQPTAAVSTSTVTFTIDDCNEEGDAIPAAASVPAPVTMPAISPTPATLTCTTTTTMAGDSVAVSPLSMELKSRLGSHHSSMRSVVSGYLPGLSDSSGNLVGGVSMATSGLQAPNPLYMQPQASLSGSSYHFHELAGNQIYSDVTSVRSLASIGIGSTDGRKLVIRRVPTTANELFDMVNPQTPPPLGVDDDDSYLDMSDETAQLKPRQQHWANKMQFVLACIGYSVGLGNVWRFPYMCYKSGGGVFLVPYCIILFICSIPLLFMELSVGQYTGRGPIGALGQLCPLFKGAGLASVVVSFLMSTYYSVIIGYSIYYFFTSFKTEMPWIDCNNRWNTPDCWVPQRKGINASAPDTSRTPSEEFFENKVLQISGGLEYPGMMRWELFACLICAWLMVYFATWKSIKSSAKVRYFTATFPFVLIIILMVRAVTLDGAAEGLRFFFRPKWSELKNANVWINAASQNFNSLGITFGSMISFASYNKYNNNILRDTVAVSAVNMITSLLVGIFAFSTLGNLALEQNTNVRDVIGDGPGMIFVVYPQAMAKMPYAQLWAVMFFFMLLCLGLNSQFAIVEVVVTSIQDGFPRWIKRHLGYHEIVVLFVCVISCLFGMPNIIQGGIYYFQLMDHYAASVTIMFLAFCQMIAIAWFYGTGRLSKNVKQMTGKAPSFYLRSCWLVLGPCLLFAIWVLSLINYHEPTYHNGRYTYPDWAYGIGWMFASFSLICIPGYAVINFLRSSGDTFWERIRNTLRPNIYECKICGEHHCEHDYPEQEQFMLAQEMATVYKPTNPHLLNLGQKCGYNAMQASPSHAEAGGPCGQ

Target ORF information:

RefSeq Version	NM_175958.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inebriated (ine), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175958.3

ORF Insert Sequence:

ATGGCGGAGA ACAAAGACGT CAGCCAGGTG CTAAACACCA CACCCGACCA GGTGGTCATC 
AATCGTGCCC CGCCCACCGG ACTAGCGCCC TTGAGGCACA GCCAACTCTC TGATAGCGGA 
GCGGAGAGCT GCTACGAGGG CGATGAGCAG GCACGGCTCA TCCGGTCCTC ATCGTCGCGG 
GCGCACAAGT TCATCATAAT ACCGGCCACG CCAGGAACAC CGCCGGGAAG CGGTGCGGTA 
GCAGGACCAC TTCCCTTTCG CCAGACCAGC TCAACCACAT CGCTGGCGGC CATGGCAGCT 
GCGCTGCACA AACAGTCTCC ACTGCGCCAT ACATCGGTGC GAACCAGGCC CAGTTCGGAG 
GTATTGCAAC CGGGCCAGGT GCTCGCCAAT CAGCCAACGG CAGCTGTATC CACCTCCACG 
GTGACCTTCA CCATTGACGA CTGCAATGAA GAAGGCGATG CCATTCCTGC TGCAGCTTCA 
GTTCCAGCTC CCGTGACCAT GCCCGCCATC TCACCCACGC CGGCCACCTT GACCTGCACC 
ACCACCACCA CCATGGCCGG TGATTCGGTG GCCGTCTCGC CGCTCAGCAT GGAGCTGAAG 
TCGCGGTTAG GCAGCCATCA CAGCAGCATG CGCTCCGTAG TCTCAGGTTA TCTTCCAGGC 
CTGAGCGACA GTAGCGGCAA TCTGGTTGGA GGCGTATCGA TGGCCACTTC CGGATTACAG 
GCCCCCAATC CGCTCTACAT GCAACCACAG GCCTCGCTGA GCGGCAGTTC CTATCATTTC 
CACGAACTGG CCGGCAACCA GATCTACTCC GATGTGACCT CCGTTCGCTC GCTGGCCTCC 
ATTGGCATTG GGTCGACGGA TGGGCGCAAG CTGGTCATCC GGCGAGTGCC CACCACCGCC 
AACGAGCTGT TCGACATGGT CAATCCGCAG ACGCCACCAC CCCTGGGGGT GGATGACGAT 
GACAGCTACC TGGACATGTC CGATGAGACG GCCCAGCTCA AGCCGCGCCA GCAGCACTGG 
GCCAATAAGA TGCAATTCGT CCTGGCCTGC ATAGGATACT CGGTGGGTCT GGGCAACGTC 
TGGCGTTTTC CGTACATGTG CTACAAGAGT GGCGGTGGTG TTTTTCTAGT ACCTTATTGC 
ATAATACTGT TCATATGCAG CATTCCGCTG CTCTTCATGG AGCTGTCCGT TGGCCAATAC 
ACCGGTCGAG GACCCATTGG AGCACTGGGT CAACTGTGTC CTCTGTTCAA GGGAGCCGGA 
CTCGCCAGCG TGGTCGTCTC GTTCCTCATG TCCACCTACT ATAGTGTCAT CATAGGCTAC 
TCCATCTACT ACTTCTTTAC GTCGTTCAAA ACGGAAATGC CCTGGATCGA CTGCAACAAC 
AGGTGGAACA CGCCCGATTG CTGGGTGCCA CAGCGCAAGG GGATTAATGC CAGTGCGCCG 
GACACATCTC GCACTCCATC CGAGGAATTC TTCGAAAACA AGGTCCTGCA GATCAGCGGT 
GGCCTGGAGT ATCCGGGCAT GATGCGCTGG GAGCTATTTG CCTGCCTGAT CTGCGCCTGG 
CTAATGGTCT ACTTCGCCAC GTGGAAGTCG ATCAAGTCGT CGGCGAAGGT GCGCTACTTC 
ACGGCCACGT TTCCGTTCGT GCTGATCATC ATCCTGATGG TGCGCGCGGT GACCCTGGAT 
GGAGCTGCTG AGGGTTTGCG CTTCTTCTTC CGTCCCAAGT GGTCGGAGCT GAAGAACGCC 
AATGTGTGGA TCAACGCCGC ATCCCAGAAC TTCAACTCGC TGGGCATTAC ATTCGGATCC 
ATGATCTCCT TTGCCAGCTA CAACAAGTAC AACAACAATA TCCTGCGAGA TACCGTGGCA 
GTGAGTGCGG TGAATATGAT CACCAGTCTC CTGGTGGGCA TCTTTGCCTT CTCCACGCTG 
GGCAATCTGG CGCTGGAGCA GAACACCAAT GTGAGGGACG TCATCGGTGA TGGACCGGGC 
ATGATATTCG TGGTGTATCC CCAGGCGATG GCTAAGATGC CGTACGCTCA GCTCTGGGCG 
GTCATGTTCT TCTTCATGCT GCTCTGCCTA GGTCTGAACT CGCAGTTTGC CATTGTGGAG 
GTTGTGGTCA CGTCCATACA GGATGGCTTT CCGCGGTGGA TCAAGCGGCA CCTGGGCTAT 
CACGAAATCG TGGTCCTGTT CGTCTGTGTC ATCTCATGTC TCTTCGGGAT GCCCAACATC 
ATACAGGGTG GCATCTACTA CTTCCAGCTA ATGGATCACT ACGCCGCCTC CGTGACGATC 
ATGTTTCTGG CCTTCTGTCA AATGATTGCC ATTGCCTGGT TCTACGGCAC GGGAAGACTG 
TCGAAGAACG TCAAGCAAAT GACCGGAAAG GCGCCTTCGT TCTATCTCAG ATCCTGCTGG 
CTAGTCCTGG GACCCTGCCT CCTCTTTGCC ATCTGGGTAT TGAGTCTGAT CAACTACCAC 
GAGCCCACCT ACCACAATGG ACGCTATACC TATCCGGACT GGGCCTACGG AATAGGCTGG 
ATGTTCGCTT CATTCTCGCT GATCTGCATA CCTGGATATG CGGTTATCAA CTTCCTGCGC 
TCCAGTGGAG ATACCTTTTG GGAACGCATC CGGAACACGC TGAGACCCAA CATCTACGAG 
TGCAAGATTT GCGGGGAACA CCACTGCGAA CACGACTATC CGGAGCAGGA GCAGTTTATG 
CTGGCCCAGG AAATGGCCAC AGTGTACAAG CCCACCAACC CACACCTGCT CAATCTCGGC 
CAGAAGTGCG GCTACAACGC CATGCAAGCC TCACCCTCAC ATGCGGAGGC AGGAGGACCA 
TGTGGCCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ine cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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