TTLL4B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTLL4B
Entrez Gene ID	33609
Full Name	CG3964 gene product from transcript CG3964-RB
Synonyms	Dmel\CG3964
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24C8-24C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_164562.2	NP_722946.1	tubulin tyrosine ligase-like 4B, isoform B
NM_001273081.2	NP_001260010.1	tubulin tyrosine ligase-like 4B, isoform C
NM_134966.3	NP_608810.1	tubulin tyrosine ligase-like 4B, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TTLL4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTLL4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13688	NM_001273081.2 Latest version!	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant C (TTLL4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13688	NM_164562.2 Latest version!	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant B (TTLL4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa13689	NM_134966.3 Latest version!	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant A (TTLL4B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTLL4B ( NM_164562.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722946.1 Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant B (TTLL4B), mRNA.

Price & Availability↑

CloneID	OFa13688
Clone ID Related Accession (Same CDS sequence)	NM_164562.2 , NM_001273081.2
Accession Version	NM_164562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tubulin tyrosine ligase-like 4B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164562.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGAACCA 
AATGTTGCAC TTACCGAGAT CGATATGCTG CACGTGCGAA AGGATGAGGT CTTGGACGGT 
GCCACCACGC TGGCCATACT TAAGCCCGTT GTCCAGGAGG GCTGCGAGGT GGCGCCGATT 
CCGGATGCCT GCATCGAGAT CAAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722946.1
CDS	185..3154
Translation	MAQMTEKRTTSARSGTAAKENHSLTGRQNQQNHHNQGHQRSGKGIEILDFHESWLEEVELYKDKMVMCKKLAHHNHVPASSHHLQPHLAAPNRNHGRIVQSSTPQHRSSAGGGSAAGATASDSVSARYSPPGQKQRSPPSPRKRLTPEKVANLGGGGGGSKVGAQRIIIRGTPPNSSTPKVAATPSNVPVVANSVLQSEPYKKVQMLLKRAREEAFAKHTAGNSAANGNGPKRSAHSPPIQAKVPPSNMSCPSTQTLKPSPPSKIVVNSFHGSSMLSTQQQEEKSRLLTQNGLTKSAATALETSLYASLYANAPQNSQPQFHSPRISGGDHVASYVGVSLNKGAGHDNDRKHEDDPCAAIDDINYYDDSDSEEEYVGTPFFLEEDNTETRQSKHTRLLPQKTPSKESVRTTDSEASVYFRPEAILSPSLFPHVPPYLNFTSHADKGPPMPAALHRVLKWKLSPVMPKIVKRVVLNSGFRIIKNTTDWMAVWEKHMKSPGFRTIRSHQKYNHIPGSFRIGRKDTMWRSIYNNMKKFGKKEFGFMQKSYIMPDDLESLRQVWPKNASKLTKWIVKPPASARGTGIRIVNKWSQFPKDRPLVVQKYIERPLLINDNKFDMRLYVVLTSINPLRIYMYKDGLARFASVKYSSELSNLDERCMHLTNYSINKFSQNYAKNEDFNACQGHKWTLQSLWSCLENRGVNTKRLWATLRNLVIKGIVSGESGLNRMYRQNVNFRYNCFELFGFDVLLDENLVPWLLEINISPSLHSELPLDLHVKGPLIQAVLNTALYQVPPKLNERQQADILEELNLTGPLCHDKRIFTTCLTAEEVRKHNQYTNRSIEFREEYVDTILDNLLPDDVRCLIVAEDELARCQPLERIFPTAGTHVYLRYVENARYYNRLLDAWETKYAKNREMGIALLSRYCRDKYHLQVSDAAMEKEPNVALTEIDMLHVRKDEVLDGATTLAILKPVVQEGCEVAPIPDACIEIKS

Target ORF information:

RefSeq Version	NM_164562.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant B (TTLL4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164562.2

ORF Insert Sequence:

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGAACCA 
AATGTTGCAC TTACCGAGAT CGATATGCTG CACGTGCGAA AGGATGAGGT CTTGGACGGT 
GCCACCACGC TGGCCATACT TAAGCCCGTT GTCCAGGAGG GCTGCGAGGT GGCGCCGATT 
CCGGATGCCT GCATCGAGAT CAAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4B ( NM_001273081.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260010.1 Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant C (TTLL4B), mRNA.

Price & Availability↑

CloneID	OFa13688
Clone ID Related Accession (Same CDS sequence)	NM_164562.2 , NM_001273081.2
Accession Version	NM_001273081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tubulin tyrosine ligase-like 4B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273081.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGAACCA 
AATGTTGCAC TTACCGAGAT CGATATGCTG CACGTGCGAA AGGATGAGGT CTTGGACGGT 
GCCACCACGC TGGCCATACT TAAGCCCGTT GTCCAGGAGG GCTGCGAGGT GGCGCCGATT 
CCGGATGCCT GCATCGAGAT CAAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260010.1
CDS	266..3235
Translation	MAQMTEKRTTSARSGTAAKENHSLTGRQNQQNHHNQGHQRSGKGIEILDFHESWLEEVELYKDKMVMCKKLAHHNHVPASSHHLQPHLAAPNRNHGRIVQSSTPQHRSSAGGGSAAGATASDSVSARYSPPGQKQRSPPSPRKRLTPEKVANLGGGGGGSKVGAQRIIIRGTPPNSSTPKVAATPSNVPVVANSVLQSEPYKKVQMLLKRAREEAFAKHTAGNSAANGNGPKRSAHSPPIQAKVPPSNMSCPSTQTLKPSPPSKIVVNSFHGSSMLSTQQQEEKSRLLTQNGLTKSAATALETSLYASLYANAPQNSQPQFHSPRISGGDHVASYVGVSLNKGAGHDNDRKHEDDPCAAIDDINYYDDSDSEEEYVGTPFFLEEDNTETRQSKHTRLLPQKTPSKESVRTTDSEASVYFRPEAILSPSLFPHVPPYLNFTSHADKGPPMPAALHRVLKWKLSPVMPKIVKRVVLNSGFRIIKNTTDWMAVWEKHMKSPGFRTIRSHQKYNHIPGSFRIGRKDTMWRSIYNNMKKFGKKEFGFMQKSYIMPDDLESLRQVWPKNASKLTKWIVKPPASARGTGIRIVNKWSQFPKDRPLVVQKYIERPLLINDNKFDMRLYVVLTSINPLRIYMYKDGLARFASVKYSSELSNLDERCMHLTNYSINKFSQNYAKNEDFNACQGHKWTLQSLWSCLENRGVNTKRLWATLRNLVIKGIVSGESGLNRMYRQNVNFRYNCFELFGFDVLLDENLVPWLLEINISPSLHSELPLDLHVKGPLIQAVLNTALYQVPPKLNERQQADILEELNLTGPLCHDKRIFTTCLTAEEVRKHNQYTNRSIEFREEYVDTILDNLLPDDVRCLIVAEDELARCQPLERIFPTAGTHVYLRYVENARYYNRLLDAWETKYAKNREMGIALLSRYCRDKYHLQVSDAAMEKEPNVALTEIDMLHVRKDEVLDGATTLAILKPVVQEGCEVAPIPDACIEIKS

Target ORF information:

RefSeq Version	NM_001273081.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant C (TTLL4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273081.2

ORF Insert Sequence:

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGAACCA 
AATGTTGCAC TTACCGAGAT CGATATGCTG CACGTGCGAA AGGATGAGGT CTTGGACGGT 
GCCACCACGC TGGCCATACT TAAGCCCGTT GTCCAGGAGG GCTGCGAGGT GGCGCCGATT 
CCGGATGCCT GCATCGAGAT CAAGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4B ( NM_134966.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608810.1 Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant A (TTLL4B), mRNA.

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CloneID	OFa13689
Clone ID Related Accession (Same CDS sequence)	NM_134966.3
Accession Version	NM_134966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tubulin tyrosine ligase-like 4B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134966.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGTGAAC 
GATCAATTCA ATTCAATTGA ATTTCCCCTT CCGTACCGCT TCTGTAGTCC ATTTTCAAGT 
CATATTTCTT ATCTAGGAAC CAAATGTTGC ACTTACCGAG ATCGATATGC TGCACGTGCG 
AAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608810.1
CDS	185..3133
Translation	MAQMTEKRTTSARSGTAAKENHSLTGRQNQQNHHNQGHQRSGKGIEILDFHESWLEEVELYKDKMVMCKKLAHHNHVPASSHHLQPHLAAPNRNHGRIVQSSTPQHRSSAGGGSAAGATASDSVSARYSPPGQKQRSPPSPRKRLTPEKVANLGGGGGGSKVGAQRIIIRGTPPNSSTPKVAATPSNVPVVANSVLQSEPYKKVQMLLKRAREEAFAKHTAGNSAANGNGPKRSAHSPPIQAKVPPSNMSCPSTQTLKPSPPSKIVVNSFHGSSMLSTQQQEEKSRLLTQNGLTKSAATALETSLYASLYANAPQNSQPQFHSPRISGGDHVASYVGVSLNKGAGHDNDRKHEDDPCAAIDDINYYDDSDSEEEYVGTPFFLEEDNTETRQSKHTRLLPQKTPSKESVRTTDSEASVYFRPEAILSPSLFPHVPPYLNFTSHADKGPPMPAALHRVLKWKLSPVMPKIVKRVVLNSGFRIIKNTTDWMAVWEKHMKSPGFRTIRSHQKYNHIPGSFRIGRKDTMWRSIYNNMKKFGKKEFGFMQKSYIMPDDLESLRQVWPKNASKLTKWIVKPPASARGTGIRIVNKWSQFPKDRPLVVQKYIERPLLINDNKFDMRLYVVLTSINPLRIYMYKDGLARFASVKYSSELSNLDERCMHLTNYSINKFSQNYAKNEDFNACQGHKWTLQSLWSCLENRGVNTKRLWATLRNLVIKGIVSGESGLNRMYRQNVNFRYNCFELFGFDVLLDENLVPWLLEINISPSLHSELPLDLHVKGPLIQAVLNTALYQVPPKLNERQQADILEELNLTGPLCHDKRIFTTCLTAEEVRKHNQYTNRSIEFREEYVDTILDNLLPDDVRCLIVAEDELARCQPLERIFPTAGTHVYLRYVENARYYNRLLDAWETKYAKNREMGIALLSRYCRDKYHLQVSDAAMEKVNDQFNSIEFPLPYRFCSPFSSHISYLGTKCCTYRDRYAARAKG

Target ORF information:

RefSeq Version	NM_134966.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tubulin tyrosine ligase-like 4B, transcript variant A (TTLL4B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134966.3

ORF Insert Sequence:

ATGGCTCAGA TGACCGAGAA GAGGACAACG TCGGCCCGAT CTGGTACTGC CGCCAAGGAG 
AACCACAGCC TGACGGGACG GCAGAACCAG CAGAATCATC ACAATCAGGG ACACCAGCGC 
AGCGGCAAGG GCATCGAGAT ACTCGACTTC CACGAATCGT GGCTGGAGGA GGTGGAGCTG 
TACAAGGACA AGATGGTCAT GTGCAAGAAG CTGGCCCATC ACAATCACGT GCCCGCCTCA 
TCGCACCACC TGCAGCCGCA CCTGGCCGCC CCGAATCGAA ATCATGGCAG AATCGTCCAG 
TCCAGCACCC CGCAGCATCG CAGCTCGGCC GGCGGAGGAT CCGCGGCTGG AGCCACAGCA 
TCCGATTCCG TCTCGGCTCG CTACTCGCCG CCTGGTCAAA AGCAAAGATC ACCGCCCTCG 
CCGCGCAAAC GGCTGACGCC GGAGAAGGTG GCCAACTTAG GAGGAGGAGG CGGTGGCTCT 
AAGGTAGGCG CGCAGCGCAT CATCATCCGT GGAACGCCGC CCAACAGCTC CACACCCAAG 
GTAGCCGCCA CGCCCTCAAA TGTTCCCGTC GTGGCCAACA GTGTGCTCCA ATCGGAACCG 
TACAAGAAAG TCCAGATGCT ATTGAAGCGC GCAAGGGAGG AGGCCTTCGC CAAGCACACG 
GCTGGCAATT CAGCCGCGAA TGGCAACGGA CCCAAGCGCT CCGCCCACAG TCCGCCCATT 
CAGGCCAAGG TACCGCCGTC GAACATGTCC TGCCCAAGCA CCCAGACGCT TAAGCCGTCG 
CCGCCCAGCA AGATTGTGGT GAACTCGTTC CACGGCAGCA GCATGCTGAG CACGCAGCAA 
CAGGAGGAGA AGAGCAGACT CCTCACCCAA AACGGGCTGA CCAAGTCGGC GGCCACCGCT 
TTGGAAACGA GTCTCTACGC CTCGCTGTAC GCCAATGCGC CACAGAACAG CCAGCCGCAG 
TTCCATAGCC CGCGGATTTC CGGAGGAGAT CACGTCGCGT CCTATGTGGG CGTCTCACTG 
AACAAGGGAG CCGGACACGA TAACGACAGG AAGCACGAAG ATGATCCGTG CGCAGCCATC 
GACGACATTA ACTACTACGA CGATTCAGAC TCGGAGGAGG AGTATGTGGG CACGCCATTC 
TTTCTGGAGG AGGACAACAC GGAGACGCGC CAGAGCAAGC ACACTCGATT GCTGCCGCAA 
AAGACACCAT CGAAGGAATC TGTGCGCACC ACGGACTCGG AGGCGTCTGT CTATTTCCGT 
CCGGAGGCCA TACTCTCGCC CAGTCTCTTC CCGCACGTTC CGCCGTACCT GAACTTTACG 
TCCCACGCGG ACAAGGGTCC TCCAATGCCG GCAGCATTGC ATCGAGTGCT CAAATGGAAA 
CTCAGCCCCG TGATGCCGAA GATTGTCAAG CGAGTGGTGC TCAATTCGGG CTTCCGCATC 
ATCAAGAACA CCACCGACTG GATGGCCGTG TGGGAGAAGC ACATGAAGAG CCCCGGGTTT 
AGGACCATTC GATCGCACCA GAAGTACAAC CATATACCCG GATCGTTCCG CATCGGTAGA 
AAAGACACCA TGTGGCGCAG CATCTACAAC AATATGAAGA AGTTCGGCAA GAAGGAGTTT 
GGCTTTATGC AAAAATCCTA CATTATGCCA GACGATCTGG AGAGCCTGCG TCAGGTGTGG 
CCCAAAAATG CCTCGAAGCT GACCAAGTGG ATTGTTAAGC CGCCGGCGAG TGCCCGAGGC 
ACGGGCATAC GCATTGTGAA CAAGTGGAGC CAATTTCCCA AAGATCGTCC TCTGGTGGTT 
CAGAAATACA TTGAACGTCC GCTGTTGATC AACGACAATA AGTTCGATAT GCGTCTGTAT 
GTGGTACTGA CCTCGATCAA CCCACTCCGC ATCTATATGT ACAAGGATGG ACTGGCGCGT 
TTCGCGTCCG TTAAGTACAG CTCGGAGCTG AGCAATCTGG ACGAGCGCTG CATGCACCTG 
ACCAACTACA GCATCAACAA GTTCTCGCAG AACTATGCGA AGAACGAGGA TTTCAATGCC 
TGCCAGGGCC ACAAGTGGAC GCTTCAATCG CTGTGGTCCT GCCTGGAGAA TCGTGGCGTG 
AACACTAAGC GCCTGTGGGC CACGCTACGG AATCTGGTCA TCAAGGGCAT CGTGAGCGGG 
GAATCGGGAT TGAATCGCAT GTACCGCCAG AATGTGAACT TTCGCTACAA TTGCTTCGAG 
TTGTTTGGCT TCGATGTGCT CCTGGATGAG AATCTAGTGC CCTGGCTGTT GGAGATAAAT 
ATATCGCCGT CGTTGCATTC GGAATTGCCA CTGGATCTGC ACGTGAAGGG TCCGCTCATT 
CAGGCCGTGC TGAATACGGC TCTATATCAG GTGCCGCCTA AGCTGAACGA ACGTCAGCAG 
GCGGACATCC TGGAGGAGCT TAATCTGACC GGTCCGTTGT GCCATGACAA ACGCATCTTC 
ACCACCTGCC TGACTGCAGA GGAGGTCCGC AAACATAACC AGTACACCAA TCGGAGCATC 
GAATTCCGGG AGGAGTACGT TGATACCATA CTGGACAATC TACTGCCGGA CGATGTCCGT 
TGCCTGATCG TTGCCGAGGA CGAGCTGGCC AGGTGTCAGC CGCTGGAACG GATCTTCCCC 
ACCGCCGGCA CACATGTATA TCTGCGCTAT GTCGAGAATG CCCGGTACTA TAATCGCCTG 
CTGGACGCCT GGGAGACGAA GTACGCCAAG AATAGGGAAA TGGGCATTGC CTTGCTGTCG 
CGCTATTGCC GGGACAAATA CCATTTACAG GTGTCGGACG CCGCCATGGA AAAGGTGAAC 
GATCAATTCA ATTCAATTGA ATTTCCCCTT CCGTACCGCT TCTGTAGTCC ATTTTCAAGT 
CATATTTCTT ATCTAGGAAC CAAATGTTGC ACTTACCGAG ATCGATATGC TGCACGTGCG 
AAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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TTLL4B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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