bark cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bark
Entrez Gene ID	33604
Full Name	bark beetle
Synonyms	CG3921,Dmel\CG3921,aka
General protein information	Preferred Names bark beetle Names CG3921 gene product from transcript CG3921-RB CG3921-PA CG3921-PB CG3921-PC anakonda bark-PA bark-PB bark-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 24C4-24C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_134964.4	NP_608808.1	bark beetle, isoform A
NM_001258935.3	NP_001245864.1	bark beetle, isoform B
NM_001258936.2	NP_001245865.1	bark beetle, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	SCRAC1	XP_319903.4
Drosophila melanogaster (fruit fly)	CG3921	NP_001245865.1
Caenorhabditis elegans (roundworm)	CELE_C06B8.7	NP_506859.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study describes the identification and functional characterisation of the putative scavenger receptor-like protein Bark beetle (Bark; CG3921) Bark is required for septate junction (SJ) maturation in developing epithelial cells, in addition Bark controls epithelial cell adhesion within the SJ compartment.
Title: Bark beetle controls epithelial morphogenesis by septate junction maturation in Drosophila.

The following bark gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bark cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13684	NM_134964.4 Latest version!	Drosophila melanogaster bark beetle (bark), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa16171	NM_001258936.2 Latest version!	Drosophila melanogaster bark beetle (bark), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa16171	NM_001258935.3 Latest version!	Drosophila melanogaster bark beetle (bark), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bark ( NM_134964.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608808.1 Drosophila melanogaster bark beetle (bark), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13684
Clone ID Related Accession (Same CDS sequence)	NM_134964.4
Accession Version	NM_134964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bark beetle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134964.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATGACC 
TTTGAAACCT GGAGCTCTAA GATCGACGCT CGTCACAATT ATTGGAGCTA TAATAATACC 
ATCTCGGTGC AATCTCGTAT TAGAGATAAG TCAGATGATC CTATGCTACT GGAGGTCTTA 
GCCGTGCCCT TCCAAATGAA CAATGAGACA ATACTGGATG GTAAATGTCC TCCAGGTTGG 
GCACTGGTCC ATGACACCTG TTTTATCTAC GTGGGAGCTC CCATGACTTT CCACGAAGCT 
CGAGATTTCT GTCGCTCGGA GAACTCCACA ATGCCCTTCA TACGCACCGA TAAGACGACG 
CTATGGAAGT ATTTGCAGAG CCAGATGCGA CACTTGAAAT ATCCGGATAA GGTTTGGATT 
CAGGACTATA ATCACATAGA TCGCTGCACG AGCTTTGTGT TTGGGGAAAT CGAAATTGAG 
GATTGCAACA AGGAGCGCGG TTTTATTTGC GAATCGGATC CCAGGGTTAT CATTGATCCC 
CTATCCTGGC GTGCAGACAT CTTTGCCATC AGTATTATCT CAGCCTTTGT CCTTGCCATT 
ATATTGCTGA TCCTGGTGGC CTTCTGTTGG TTCGCTAAAT CAAAACATCG ACATACCCAA 
CGACTCCAGA GAAGAAACAG CATTCGCCAA AGTCTGCGCA GCTTGAACAG CATCGATCCA 
CAGGGTTCCC TTCGCAGAAG ACCCAATTAT AATATGTCCA GCAATGGAAC ATTGTCCAAG 
GGTCAGGACT ACAAGCAGAT GGTGGCCAAT GGTTCCATAG ATTCCATGGA CAAAAGTGTA 
TTAAGCTCGG AAGCTGGTAG CTTTGAGGGC TATGAACAGA AGCCCCACTA CAATGAGTAT 
GTGAATCAGA ATGCCTTGCG ACCAGCACAT CCTGCACAGG ATCATCAGAG TCACAAGGTG 
GCCACCATTT CCAAGGCGAG TGGCCATAGA GCGAGAGCCG CTGCAGCTGC TGCTGCGGCC 
TTGGAGCCCG ACGCCTTTGA GTTAAGCTAC CGAAACGAAG GATTCCGGGA TAACTCCACC 
TACGGTGATA ACACCCGAGC CAATTCCATT AGTACCAGTG TGGCAGAGGA CACGCCAATC 
ATACATCACA CAGATCAGGA GATCGATGAG GGTGGTTCCG ATTATTATGG AAATGCGAGC 
ACCTTGCCAC TTCGAACCGA GGGAGGAACT CCGGCTGGAA GGCGTGGCCA ACCCGGCTTG 
GCCTTTCTCT CCGAACTCAA ACAGAACCTG CCCGAGTACC AAAGAAGCTC GCATAGCAGT 
TTTATGCCCC ACCGAAGCAG CGGCGACTCA CTGCCCTTTG ATCAGAAGCT AGATCAATTC 
AACTACTCTA CAGAGTCCTC ACTGTACCGC CCAGCGCCTG CGGTTCCCAG TAGTCAGCAA 
GCAACACCGG CGGACATGCG ACGTCCGGAC TCGTATTATA CTGCCGTCAG GAGTAGTAAA 
GCTCCAGTAT CACATTACCG GACACCGAGG CCACTGGCAC AGCCACCGGC TGCACCAAAT 
GTGGCGCCCG CGGGTGGACC GGCGCAACGG CGTCCCAAGA CGGTTTACCA AACCGCATCC 
GAGGAGTCGT CGCCAACGAC ACCGTCACCA CTGACCAATC AGTACCACCG CTCCAAATCG 
GAGGCTCTAC TCGAAACAGA TTTCGATGGG GATGGAGGAT CGGTTGGTCT GCAGCCACTG 
CAAACAAACG GACGAAGTCA CAGTCAGCCC CTGGAAACGG CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608808.1
CDS	278..9625
Translation	MKLQHHKTNRQRISKPHRDPKWASICLWLLVTLAFSTHLARSQESRQTEDSKEVELLQDNDIEFASLDGASQLLPATRHSGADVTVAPQGSTPSMTSSSSYTELQGGEILSDRILRRSESPYLARDDIEVLRGARLTIEPGVTIEFAPTKGLKINGVLQAVGTPTSRIVLKSQSNTANYKLELPDDQEKGIRLVDGPTPVEGRLQLFHKGAWRSVCSNSRNWTLADYGVACKQLGYRGGRFWNWVERTPGYYPRLLYEQPKCKGGEGSLQDCAWTSRQMGAGACDYHNDLGIQCLPVHSETLGHWRGIYFDNAPSTKALGRDNIVYAAQSESRLKYVDIIRAGSGAGFNAKSAVEVQGLPPQMEHVVISHSAYTGFNSTRPWAGFQLQNVTVRKCNGIGVFVNSSQGAVQLDGCSIVDNAGDGIKYVGHDLRGTERKDRASIYDFCTLPTTSGQTYPISLSFTQKYYAGSGKECGKYFFTRPGYLLTLHFENFVLMQNETATVEIYDGASTNDRLLFEWKARNFTRPQSVTSTREKMFVRIRADARQELNGFFRMTSGDSVAYDLKVSQSTVEDNGGRGVAIDNIRSKLHVHSSSVSGNGHVAGVHVTSGAGDVNITSSNISFNNGAGVNITYYGGNRNISRSALTANKGYGVATWLNQTSDVNRMEYIPFNQTSVVEYSQIGGNLETGVFHGNFCRPIWVNITGNSFNGSQQNDIFIESCYQATANGRPNMQLQLGHNQFKYSQANSIYLSPALNLQGRIEYNMFRFGSYGCLFINNDYIYPEFNYFPVKLIIQSNYFMRNSGVHVVSLGLSPYSRAEVQYILFTRNFVRGNNITEAFGPLIAGSEGSDGAGRLNPRSRVAAPVVVGSSNVDIFRNILHNLDSMYEIGSQLTDQSKIINATCNWLGHTDENKIYARLFHRNDRYNLAKINYLPYLLHSSNPGSTAMITVSTVVPRFFHEGSDVIGGEVDGQDMVPAGTYTVTKDINIRPGGKLILQPGTTLKFEPSVGMMVAGKLEARGRRPDDILFTLKRETIMGESNDTETIDLDSETEAIDMETEVIPADGVPRVPVRLVGGAGANEGRLQVYLKGRWGTVCDYGWNVLNAALVCHQLGYSLNPQDWRLLRSQLPNAGTSEDILMANVRCTLQDRDVTKCRAEYEFENTCSHENDVGLRCYEGAWAGVRFSMLAERADLQYVTVEKAGLFDYTTNAFKPAVQMDHARHNLENVRIVNNLQDGLGIIYADIYAGKSVNNIKNSEFSGNKGSGISLKQLDFRVSGSIIKDNKGSGVSHDAVISALDQKEIGGWFNMATDFNSFDTDYDPYLLPREISNIDLGTFEHKYIRTEELLGQNINRKIVVQCPAGYVIGIQLLNPIHNLSTESINILNARTENIRSDLWQVKRDLNVFPVTSSSYGIIIYYESGLQALGGAVLMLSTVTAPVQNIRNRIVSGAVPTLTIRSTKIQKNLRGITGIYYNRYIGDNGEYYLRKANESIKLINSELSYNEREAILIRSPFWDVISSNLSEVTLHVNGSLITQNGLGIRQMSKDLRSSNNLFHYVIQDTTFEQNTHGGFQVSLPYVWQYNENFTHSIYFGNSTWQRNRDFRISVSGHYTVFNITSNVFRENNCPGALISLDGMEKRLRFDNNRFESNNAKFVLLFKADSLSEIIGQVPASIEFNSFKGNNIVTMTANYRNHYMKVARRIRKQHKIPTAVIRLDGVQNVRLYRNLIAENEMDYNLVAGVRSARLNNYFEARENWWGTKDTAFIEAKIFDFDNWNDHADVIYQPFLIEDSYDASVSVVVPFNQDQEIDLTNYKGGRVYKDLLLTKQSTPYYISSDITVMPGKTLTINHGVTMEFEPNVGILVLGTLVAIGYRESPIVMRPFRNATRESLIDVQPKKRALEDMSAPLTEFDSIRLCTSANNCTGDADGLFGLNEGFLEYFNHTTLQWVPICDSRFTERNAQVVCRELGYDPLNVYYGHDRRIEFHTNSLTRIWSWVQPLECRGDEERMEDCAERLNGQLYGHRHECRWDDVFVFVSCNGIADDEVYWGGIRFANSKFEEIQYEHRLHNTRSHARLPLRESQLEFVRIEQAGILHNHKAAAIQAIHKNPSITSVSIENSANHGINMIAPSGKLNLNHLNINNTLGTGISIVSLSGEGRDSDESSFTPLKKLDLPYKLFSLVDICDPQKVLTIEERMLIYYKYDNNPVNCVKIFTSAFRAKPIGFRLLQSNLFNHSKLYGRTDFIKLYDGDIYNVTATYLGKIESDTDNQRSFFKTKGPTMSLQLVASGAPETHGFIAEVVTVPISTLGQYRDALHNITDTHISGAIKGAVTYSSAGEVTPTLTLIGNRIEKNCRQLYGNFSTCTSALNLDVQNMNSLYFMNNLITENQGGLRIRADSRGSATSLRGFVHHNLFMRNRNRPALYVEGRQSSPYQEVELYRNYFAQNMAGYEDVIRLCQVVSNFSYNYVHSNVGGRIMEVSGFEKVRLQIYQTTAHNGFYRNFATNWMTRATIVAGTAGQQYVDNIFENHENDYELLTVNNSMTFETWSSKIDARHNYWSYNNTISVQSRIRDKSDDPMLLEVLAVPFQMNNETILDGKCPPGWALVHDTCFIYVGAPMTFHEARDFCRSENSTMPFIRTDKTTLWKYLQSQMRHLKYPDKVWIQDYNHIDRCTSFVFGEIEIEDCNKERGFICESDPRVIIDPLSWRADIFAISIISAFVLAIILLILVAFCWFAKSKHRHTQRLQRRNSIRQSLRSLNSIDPQGSLRRRPNYNMSSNGTLSKGQDYKQMVANGSIDSMDKSVLSSEAGSFEGYEQKPHYNEYVNQNALRPAHPAQDHQSHKVATISKASGHRARAAAAAAAALEPDAFELSYRNEGFRDNSTYGDNTRANSISTSVAEDTPIIHHTDQEIDEGGSDYYGNASTLPLRTEGGTPAGRRGQPGLAFLSELKQNLPEYQRSSHSSFMPHRSSGDSLPFDQKLDQFNYSTESSLYRPAPAVPSSQQATPADMRRPDSYYTAVRSSKAPVSHYRTPRPLAQPPAAPNVAPAGGPAQRRPKTVYQTASEESSPTTPSPLTNQYHRSKSEALLETDFDGDGGSVGLQPLQTNGRSHSQPLETAM

Target ORF information:

RefSeq Version	NM_134964.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bark beetle (bark), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134964.4

ORF Insert Sequence:

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATGACC 
TTTGAAACCT GGAGCTCTAA GATCGACGCT CGTCACAATT ATTGGAGCTA TAATAATACC 
ATCTCGGTGC AATCTCGTAT TAGAGATAAG TCAGATGATC CTATGCTACT GGAGGTCTTA 
GCCGTGCCCT TCCAAATGAA CAATGAGACA ATACTGGATG GTAAATGTCC TCCAGGTTGG 
GCACTGGTCC ATGACACCTG TTTTATCTAC GTGGGAGCTC CCATGACTTT CCACGAAGCT 
CGAGATTTCT GTCGCTCGGA GAACTCCACA ATGCCCTTCA TACGCACCGA TAAGACGACG 
CTATGGAAGT ATTTGCAGAG CCAGATGCGA CACTTGAAAT ATCCGGATAA GGTTTGGATT 
CAGGACTATA ATCACATAGA TCGCTGCACG AGCTTTGTGT TTGGGGAAAT CGAAATTGAG 
GATTGCAACA AGGAGCGCGG TTTTATTTGC GAATCGGATC CCAGGGTTAT CATTGATCCC 
CTATCCTGGC GTGCAGACAT CTTTGCCATC AGTATTATCT CAGCCTTTGT CCTTGCCATT 
ATATTGCTGA TCCTGGTGGC CTTCTGTTGG TTCGCTAAAT CAAAACATCG ACATACCCAA 
CGACTCCAGA GAAGAAACAG CATTCGCCAA AGTCTGCGCA GCTTGAACAG CATCGATCCA 
CAGGGTTCCC TTCGCAGAAG ACCCAATTAT AATATGTCCA GCAATGGAAC ATTGTCCAAG 
GGTCAGGACT ACAAGCAGAT GGTGGCCAAT GGTTCCATAG ATTCCATGGA CAAAAGTGTA 
TTAAGCTCGG AAGCTGGTAG CTTTGAGGGC TATGAACAGA AGCCCCACTA CAATGAGTAT 
GTGAATCAGA ATGCCTTGCG ACCAGCACAT CCTGCACAGG ATCATCAGAG TCACAAGGTG 
GCCACCATTT CCAAGGCGAG TGGCCATAGA GCGAGAGCCG CTGCAGCTGC TGCTGCGGCC 
TTGGAGCCCG ACGCCTTTGA GTTAAGCTAC CGAAACGAAG GATTCCGGGA TAACTCCACC 
TACGGTGATA ACACCCGAGC CAATTCCATT AGTACCAGTG TGGCAGAGGA CACGCCAATC 
ATACATCACA CAGATCAGGA GATCGATGAG GGTGGTTCCG ATTATTATGG AAATGCGAGC 
ACCTTGCCAC TTCGAACCGA GGGAGGAACT CCGGCTGGAA GGCGTGGCCA ACCCGGCTTG 
GCCTTTCTCT CCGAACTCAA ACAGAACCTG CCCGAGTACC AAAGAAGCTC GCATAGCAGT 
TTTATGCCCC ACCGAAGCAG CGGCGACTCA CTGCCCTTTG ATCAGAAGCT AGATCAATTC 
AACTACTCTA CAGAGTCCTC ACTGTACCGC CCAGCGCCTG CGGTTCCCAG TAGTCAGCAA 
GCAACACCGG CGGACATGCG ACGTCCGGAC TCGTATTATA CTGCCGTCAG GAGTAGTAAA 
GCTCCAGTAT CACATTACCG GACACCGAGG CCACTGGCAC AGCCACCGGC TGCACCAAAT 
GTGGCGCCCG CGGGTGGACC GGCGCAACGG CGTCCCAAGA CGGTTTACCA AACCGCATCC 
GAGGAGTCGT CGCCAACGAC ACCGTCACCA CTGACCAATC AGTACCACCG CTCCAAATCG 
GAGGCTCTAC TCGAAACAGA TTTCGATGGG GATGGAGGAT CGGTTGGTCT GCAGCCACTG 
CAAACAAACG GACGAAGTCA CAGTCAGCCC CTGGAAACGG CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bark ( NM_001258935.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245864.1 Drosophila melanogaster bark beetle (bark), transcript variant B, mRNA.

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CloneID	OFa16171
Clone ID Related Accession (Same CDS sequence)	NM_001258935.3 , NM_001258936.2
Accession Version	NM_001258935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bark beetle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258935.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATTTTG 
AGTTTTGACT ATGAAAACAG GACCTTTGAA ACCTGGAGCT CTAAGATCGA CGCTCGTCAC 
AATTATTGGA GCTATAATAA TACCATCTCG GTGCAATCTC GTATTAGAGA TAAGTCAGAT 
GATCCTATGC TACTGGAGGT CTTAGCCGTG CCCTTCCAAA TGAACAATGA GACAATACTG 
GATGGTAAAT GTCCTCCAGG TTGGGCACTG GTCCATGACA CCTGTTTTAT CTACGTGGGA 
GCTCCCATGA CTTTCCACGA AGCTCGAGAT TTCTGTCGCT CGGAGAACTC CACAATGCCC 
TTCATACGCA CCGATAAGAC GACGCTATGG AAGTATTTGC AGAGCCAGAT GCGACACTTG 
AAATATCCGG ATAAGGTTTG GATTCAGGAC TATAATCACA TAGATCGCTG CACGAGCTTT 
GTGTTTGGGG AAATCGAAAT TGAGGATTGC AACAAGGAGC GCGGTTTTAT TTGCGAATCG 
GATCCCAGGG TTATCATTGA TCCCCTATCC TGGCGTGCAG ACATCTTTGC CATCAGTATT 
ATCTCAGCCT TTGTCCTTGC CATTATATTG CTGATCCTGG TGGCCTTCTG TTGGTTCGCT 
AAATCAAAAC ATCGACATAC CCAACGACTC CAGAGAAGAA ACAGCATTCG CCAAAGTCTG 
CGCAGCTTGA ACAGCATCGA TCCACAGGGT TCCCTTCGCA GAAGACCCAA TTATAATATG 
TCCAGCAATG GAACATTGTC CAAGGGTCAG GACTACAAGC AGATGGTGGC CAATGGTTCC 
ATAGATTCCA TGGACAAAAG TGTATTAAGC TCGGAAGCTG GTAGCTTTGA GGGCTATGAA 
CAGAAGCCCC ACTACAATGA GTATGTGAAT CAGAATGCCT TGCGACCAGC ACATCCTGCA 
CAGGATCATC AGAGTCACAA GGTGGCCACC ATTTCCAAGG CGAGTGGCCA TAGAGCGAGA 
GCCGCTGCAG CTGCTGCTGC GGCCTTGGAG CCCGACGCCT TTGAGTTAAG CTACCGAAAC 
GAAGGATTCC GGGATAACTC CACCTACGGT GATAACACCC GAGCCAATTC CATTAGTACC 
AGTGTGGCAG AGGACACGCC AATCATACAT CACACAGATC AGGAGATCGA TGAGGGTGGT 
TCCGATTATT ATGGAAATGC GAGCACCTTG CCACTTCGAA CCGAGGGAGG AACTCCGGCT 
GGAAGGCGTG GCCAACCCGG CTTGGCCTTT CTCTCCGAAC TCAAACAGAA CCTGCCCGAG 
TACCAAAGAA GCTCGCATAG CAGTTTTATG CCCCACCGAA GCAGCGGCGA CTCACTGCCC 
TTTGATCAGA AGCTAGATCA ATTCAACTAC TCTACAGAGT CCTCACTGTA CCGCCCAGCG 
CCTGCGGTTC CCAGTAGTCA GCAAGCAACA CCGGCGGACA TGCGACGTCC GGACTCGTAT 
TATACTGCCG TCAGGAGTAG TAAAGCTCCA GTATCACATT ACCGGACACC GAGGCCACTG 
GCACAGCCAC CGGCTGCACC AAATGTGGCG CCCGCGGGTG GACCGGCGCA ACGGCGTCCC 
AAGACGGTTT ACCAAACCGC ATCCGAGGAG TCGTCGCCAA CGACACCGTC ACCACTGACC 
AATCAGTACC ACCGCTCCAA ATCGGAGGCT CTACTCGAAA CAGATTTCGA TGGGGATGGA 
GGATCGGTTG GTCTGCAGCC ACTGCAAACA AACGGACGAA GTCACAGTCA GCCCCTGGAA 
ACGGCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245864.1
CDS	278..9649
Translation	MKLQHHKTNRQRISKPHRDPKWASICLWLLVTLAFSTHLARSQESRQTEDSKEVELLQDNDIEFASLDGASQLLPATRHSGADVTVAPQGSTPSMTSSSSYTELQGGEILSDRILRRSESPYLARDDIEVLRGARLTIEPGVTIEFAPTKGLKINGVLQAVGTPTSRIVLKSQSNTANYKLELPDDQEKGIRLVDGPTPVEGRLQLFHKGAWRSVCSNSRNWTLADYGVACKQLGYRGGRFWNWVERTPGYYPRLLYEQPKCKGGEGSLQDCAWTSRQMGAGACDYHNDLGIQCLPVHSETLGHWRGIYFDNAPSTKALGRDNIVYAAQSESRLKYVDIIRAGSGAGFNAKSAVEVQGLPPQMEHVVISHSAYTGFNSTRPWAGFQLQNVTVRKCNGIGVFVNSSQGAVQLDGCSIVDNAGDGIKYVGHDLRGTERKDRASIYDFCTLPTTSGQTYPISLSFTQKYYAGSGKECGKYFFTRPGYLLTLHFENFVLMQNETATVEIYDGASTNDRLLFEWKARNFTRPQSVTSTREKMFVRIRADARQELNGFFRMTSGDSVAYDLKVSQSTVEDNGGRGVAIDNIRSKLHVHSSSVSGNGHVAGVHVTSGAGDVNITSSNISFNNGAGVNITYYGGNRNISRSALTANKGYGVATWLNQTSDVNRMEYIPFNQTSVVEYSQIGGNLETGVFHGNFCRPIWVNITGNSFNGSQQNDIFIESCYQATANGRPNMQLQLGHNQFKYSQANSIYLSPALNLQGRIEYNMFRFGSYGCLFINNDYIYPEFNYFPVKLIIQSNYFMRNSGVHVVSLGLSPYSRAEVQYILFTRNFVRGNNITEAFGPLIAGSEGSDGAGRLNPRSRVAAPVVVGSSNVDIFRNILHNLDSMYEIGSQLTDQSKIINATCNWLGHTDENKIYARLFHRNDRYNLAKINYLPYLLHSSNPGSTAMITVSTVVPRFFHEGSDVIGGEVDGQDMVPAGTYTVTKDINIRPGGKLILQPGTTLKFEPSVGMMVAGKLEARGRRPDDILFTLKRETIMGESNDTETIDLDSETEAIDMETEVIPADGVPRVPVRLVGGAGANEGRLQVYLKGRWGTVCDYGWNVLNAALVCHQLGYSLNPQDWRLLRSQLPNAGTSEDILMANVRCTLQDRDVTKCRAEYEFENTCSHENDVGLRCYEGAWAGVRFSMLAERADLQYVTVEKAGLFDYTTNAFKPAVQMDHARHNLENVRIVNNLQDGLGIIYADIYAGKSVNNIKNSEFSGNKGSGISLKQLDFRVSGSIIKDNKGSGVSHDAVISALDQKEIGGWFNMATDFNSFDTDYDPYLLPREISNIDLGTFEHKYIRTEELLGQNINRKIVVQCPAGYVIGIQLLNPIHNLSTESINILNARTENIRSDLWQVKRDLNVFPVTSSSYGIIIYYESGLQALGGAVLMLSTVTAPVQNIRNRIVSGAVPTLTIRSTKIQKNLRGITGIYYNRYIGDNGEYYLRKANESIKLINSELSYNEREAILIRSPFWDVISSNLSEVTLHVNGSLITQNGLGIRQMSKDLRSSNNLFHYVIQDTTFEQNTHGGFQVSLPYVWQYNENFTHSIYFGNSTWQRNRDFRISVSGHYTVFNITSNVFRENNCPGALISLDGMEKRLRFDNNRFESNNAKFVLLFKADSLSEIIGQVPASIEFNSFKGNNIVTMTANYRNHYMKVARRIRKQHKIPTAVIRLDGVQNVRLYRNLIAENEMDYNLVAGVRSARLNNYFEARENWWGTKDTAFIEAKIFDFDNWNDHADVIYQPFLIEDSYDASVSVVVPFNQDQEIDLTNYKGGRVYKDLLLTKQSTPYYISSDITVMPGKTLTINHGVTMEFEPNVGILVLGTLVAIGYRESPIVMRPFRNATRESLIDVQPKKRALEDMSAPLTEFDSIRLCTSANNCTGDADGLFGLNEGFLEYFNHTTLQWVPICDSRFTERNAQVVCRELGYDPLNVYYGHDRRIEFHTNSLTRIWSWVQPLECRGDEERMEDCAERLNGQLYGHRHECRWDDVFVFVSCNGIADDEVYWGGIRFANSKFEEIQYEHRLHNTRSHARLPLRESQLEFVRIEQAGILHNHKAAAIQAIHKNPSITSVSIENSANHGINMIAPSGKLNLNHLNINNTLGTGISIVSLSGEGRDSDESSFTPLKKLDLPYKLFSLVDICDPQKVLTIEERMLIYYKYDNNPVNCVKIFTSAFRAKPIGFRLLQSNLFNHSKLYGRTDFIKLYDGDIYNVTATYLGKIESDTDNQRSFFKTKGPTMSLQLVASGAPETHGFIAEVVTVPISTLGQYRDALHNITDTHISGAIKGAVTYSSAGEVTPTLTLIGNRIEKNCRQLYGNFSTCTSALNLDVQNMNSLYFMNNLITENQGGLRIRADSRGSATSLRGFVHHNLFMRNRNRPALYVEGRQSSPYQEVELYRNYFAQNMAGYEDVIRLCQVVSNFSYNYVHSNVGGRIMEVSGFEKVRLQIYQTTAHNGFYRNFATNWMTRATIVAGTAGQQYVDNIFENHENDYELLTVNNSILSFDYENRTFETWSSKIDARHNYWSYNNTISVQSRIRDKSDDPMLLEVLAVPFQMNNETILDGKCPPGWALVHDTCFIYVGAPMTFHEARDFCRSENSTMPFIRTDKTTLWKYLQSQMRHLKYPDKVWIQDYNHIDRCTSFVFGEIEIEDCNKERGFICESDPRVIIDPLSWRADIFAISIISAFVLAIILLILVAFCWFAKSKHRHTQRLQRRNSIRQSLRSLNSIDPQGSLRRRPNYNMSSNGTLSKGQDYKQMVANGSIDSMDKSVLSSEAGSFEGYEQKPHYNEYVNQNALRPAHPAQDHQSHKVATISKASGHRARAAAAAAAALEPDAFELSYRNEGFRDNSTYGDNTRANSISTSVAEDTPIIHHTDQEIDEGGSDYYGNASTLPLRTEGGTPAGRRGQPGLAFLSELKQNLPEYQRSSHSSFMPHRSSGDSLPFDQKLDQFNYSTESSLYRPAPAVPSSQQATPADMRRPDSYYTAVRSSKAPVSHYRTPRPLAQPPAAPNVAPAGGPAQRRPKTVYQTASEESSPTTPSPLTNQYHRSKSEALLETDFDGDGGSVGLQPLQTNGRSHSQPLETAM

Target ORF information:

RefSeq Version	NM_001258935.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bark beetle (bark), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258935.3

ORF Insert Sequence:

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATTTTG 
AGTTTTGACT ATGAAAACAG GACCTTTGAA ACCTGGAGCT CTAAGATCGA CGCTCGTCAC 
AATTATTGGA GCTATAATAA TACCATCTCG GTGCAATCTC GTATTAGAGA TAAGTCAGAT 
GATCCTATGC TACTGGAGGT CTTAGCCGTG CCCTTCCAAA TGAACAATGA GACAATACTG 
GATGGTAAAT GTCCTCCAGG TTGGGCACTG GTCCATGACA CCTGTTTTAT CTACGTGGGA 
GCTCCCATGA CTTTCCACGA AGCTCGAGAT TTCTGTCGCT CGGAGAACTC CACAATGCCC 
TTCATACGCA CCGATAAGAC GACGCTATGG AAGTATTTGC AGAGCCAGAT GCGACACTTG 
AAATATCCGG ATAAGGTTTG GATTCAGGAC TATAATCACA TAGATCGCTG CACGAGCTTT 
GTGTTTGGGG AAATCGAAAT TGAGGATTGC AACAAGGAGC GCGGTTTTAT TTGCGAATCG 
GATCCCAGGG TTATCATTGA TCCCCTATCC TGGCGTGCAG ACATCTTTGC CATCAGTATT 
ATCTCAGCCT TTGTCCTTGC CATTATATTG CTGATCCTGG TGGCCTTCTG TTGGTTCGCT 
AAATCAAAAC ATCGACATAC CCAACGACTC CAGAGAAGAA ACAGCATTCG CCAAAGTCTG 
CGCAGCTTGA ACAGCATCGA TCCACAGGGT TCCCTTCGCA GAAGACCCAA TTATAATATG 
TCCAGCAATG GAACATTGTC CAAGGGTCAG GACTACAAGC AGATGGTGGC CAATGGTTCC 
ATAGATTCCA TGGACAAAAG TGTATTAAGC TCGGAAGCTG GTAGCTTTGA GGGCTATGAA 
CAGAAGCCCC ACTACAATGA GTATGTGAAT CAGAATGCCT TGCGACCAGC ACATCCTGCA 
CAGGATCATC AGAGTCACAA GGTGGCCACC ATTTCCAAGG CGAGTGGCCA TAGAGCGAGA 
GCCGCTGCAG CTGCTGCTGC GGCCTTGGAG CCCGACGCCT TTGAGTTAAG CTACCGAAAC 
GAAGGATTCC GGGATAACTC CACCTACGGT GATAACACCC GAGCCAATTC CATTAGTACC 
AGTGTGGCAG AGGACACGCC AATCATACAT CACACAGATC AGGAGATCGA TGAGGGTGGT 
TCCGATTATT ATGGAAATGC GAGCACCTTG CCACTTCGAA CCGAGGGAGG AACTCCGGCT 
GGAAGGCGTG GCCAACCCGG CTTGGCCTTT CTCTCCGAAC TCAAACAGAA CCTGCCCGAG 
TACCAAAGAA GCTCGCATAG CAGTTTTATG CCCCACCGAA GCAGCGGCGA CTCACTGCCC 
TTTGATCAGA AGCTAGATCA ATTCAACTAC TCTACAGAGT CCTCACTGTA CCGCCCAGCG 
CCTGCGGTTC CCAGTAGTCA GCAAGCAACA CCGGCGGACA TGCGACGTCC GGACTCGTAT 
TATACTGCCG TCAGGAGTAG TAAAGCTCCA GTATCACATT ACCGGACACC GAGGCCACTG 
GCACAGCCAC CGGCTGCACC AAATGTGGCG CCCGCGGGTG GACCGGCGCA ACGGCGTCCC 
AAGACGGTTT ACCAAACCGC ATCCGAGGAG TCGTCGCCAA CGACACCGTC ACCACTGACC 
AATCAGTACC ACCGCTCCAA ATCGGAGGCT CTACTCGAAA CAGATTTCGA TGGGGATGGA 
GGATCGGTTG GTCTGCAGCC ACTGCAAACA AACGGACGAA GTCACAGTCA GCCCCTGGAA 
ACGGCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bark ( NM_001258936.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245865.1 Drosophila melanogaster bark beetle (bark), transcript variant C, mRNA.

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CloneID	OFa16171
Clone ID Related Accession (Same CDS sequence)	NM_001258935.3 , NM_001258936.2
Accession Version	NM_001258936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bark beetle, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001258936.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATTTTG 
AGTTTTGACT ATGAAAACAG GACCTTTGAA ACCTGGAGCT CTAAGATCGA CGCTCGTCAC 
AATTATTGGA GCTATAATAA TACCATCTCG GTGCAATCTC GTATTAGAGA TAAGTCAGAT 
GATCCTATGC TACTGGAGGT CTTAGCCGTG CCCTTCCAAA TGAACAATGA GACAATACTG 
GATGGTAAAT GTCCTCCAGG TTGGGCACTG GTCCATGACA CCTGTTTTAT CTACGTGGGA 
GCTCCCATGA CTTTCCACGA AGCTCGAGAT TTCTGTCGCT CGGAGAACTC CACAATGCCC 
TTCATACGCA CCGATAAGAC GACGCTATGG AAGTATTTGC AGAGCCAGAT GCGACACTTG 
AAATATCCGG ATAAGGTTTG GATTCAGGAC TATAATCACA TAGATCGCTG CACGAGCTTT 
GTGTTTGGGG AAATCGAAAT TGAGGATTGC AACAAGGAGC GCGGTTTTAT TTGCGAATCG 
GATCCCAGGG TTATCATTGA TCCCCTATCC TGGCGTGCAG ACATCTTTGC CATCAGTATT 
ATCTCAGCCT TTGTCCTTGC CATTATATTG CTGATCCTGG TGGCCTTCTG TTGGTTCGCT 
AAATCAAAAC ATCGACATAC CCAACGACTC CAGAGAAGAA ACAGCATTCG CCAAAGTCTG 
CGCAGCTTGA ACAGCATCGA TCCACAGGGT TCCCTTCGCA GAAGACCCAA TTATAATATG 
TCCAGCAATG GAACATTGTC CAAGGGTCAG GACTACAAGC AGATGGTGGC CAATGGTTCC 
ATAGATTCCA TGGACAAAAG TGTATTAAGC TCGGAAGCTG GTAGCTTTGA GGGCTATGAA 
CAGAAGCCCC ACTACAATGA GTATGTGAAT CAGAATGCCT TGCGACCAGC ACATCCTGCA 
CAGGATCATC AGAGTCACAA GGTGGCCACC ATTTCCAAGG CGAGTGGCCA TAGAGCGAGA 
GCCGCTGCAG CTGCTGCTGC GGCCTTGGAG CCCGACGCCT TTGAGTTAAG CTACCGAAAC 
GAAGGATTCC GGGATAACTC CACCTACGGT GATAACACCC GAGCCAATTC CATTAGTACC 
AGTGTGGCAG AGGACACGCC AATCATACAT CACACAGATC AGGAGATCGA TGAGGGTGGT 
TCCGATTATT ATGGAAATGC GAGCACCTTG CCACTTCGAA CCGAGGGAGG AACTCCGGCT 
GGAAGGCGTG GCCAACCCGG CTTGGCCTTT CTCTCCGAAC TCAAACAGAA CCTGCCCGAG 
TACCAAAGAA GCTCGCATAG CAGTTTTATG CCCCACCGAA GCAGCGGCGA CTCACTGCCC 
TTTGATCAGA AGCTAGATCA ATTCAACTAC TCTACAGAGT CCTCACTGTA CCGCCCAGCG 
CCTGCGGTTC CCAGTAGTCA GCAAGCAACA CCGGCGGACA TGCGACGTCC GGACTCGTAT 
TATACTGCCG TCAGGAGTAG TAAAGCTCCA GTATCACATT ACCGGACACC GAGGCCACTG 
GCACAGCCAC CGGCTGCACC AAATGTGGCG CCCGCGGGTG GACCGGCGCA ACGGCGTCCC 
AAGACGGTTT ACCAAACCGC ATCCGAGGAG TCGTCGCCAA CGACACCGTC ACCACTGACC 
AATCAGTACC ACCGCTCCAA ATCGGAGGCT CTACTCGAAA CAGATTTCGA TGGGGATGGA 
GGATCGGTTG GTCTGCAGCC ACTGCAAACA AACGGACGAA GTCACAGTCA GCCCCTGGAA 
ACGGCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245865.1
CDS	219..9590
Translation	MKLQHHKTNRQRISKPHRDPKWASICLWLLVTLAFSTHLARSQESRQTEDSKEVELLQDNDIEFASLDGASQLLPATRHSGADVTVAPQGSTPSMTSSSSYTELQGGEILSDRILRRSESPYLARDDIEVLRGARLTIEPGVTIEFAPTKGLKINGVLQAVGTPTSRIVLKSQSNTANYKLELPDDQEKGIRLVDGPTPVEGRLQLFHKGAWRSVCSNSRNWTLADYGVACKQLGYRGGRFWNWVERTPGYYPRLLYEQPKCKGGEGSLQDCAWTSRQMGAGACDYHNDLGIQCLPVHSETLGHWRGIYFDNAPSTKALGRDNIVYAAQSESRLKYVDIIRAGSGAGFNAKSAVEVQGLPPQMEHVVISHSAYTGFNSTRPWAGFQLQNVTVRKCNGIGVFVNSSQGAVQLDGCSIVDNAGDGIKYVGHDLRGTERKDRASIYDFCTLPTTSGQTYPISLSFTQKYYAGSGKECGKYFFTRPGYLLTLHFENFVLMQNETATVEIYDGASTNDRLLFEWKARNFTRPQSVTSTREKMFVRIRADARQELNGFFRMTSGDSVAYDLKVSQSTVEDNGGRGVAIDNIRSKLHVHSSSVSGNGHVAGVHVTSGAGDVNITSSNISFNNGAGVNITYYGGNRNISRSALTANKGYGVATWLNQTSDVNRMEYIPFNQTSVVEYSQIGGNLETGVFHGNFCRPIWVNITGNSFNGSQQNDIFIESCYQATANGRPNMQLQLGHNQFKYSQANSIYLSPALNLQGRIEYNMFRFGSYGCLFINNDYIYPEFNYFPVKLIIQSNYFMRNSGVHVVSLGLSPYSRAEVQYILFTRNFVRGNNITEAFGPLIAGSEGSDGAGRLNPRSRVAAPVVVGSSNVDIFRNILHNLDSMYEIGSQLTDQSKIINATCNWLGHTDENKIYARLFHRNDRYNLAKINYLPYLLHSSNPGSTAMITVSTVVPRFFHEGSDVIGGEVDGQDMVPAGTYTVTKDINIRPGGKLILQPGTTLKFEPSVGMMVAGKLEARGRRPDDILFTLKRETIMGESNDTETIDLDSETEAIDMETEVIPADGVPRVPVRLVGGAGANEGRLQVYLKGRWGTVCDYGWNVLNAALVCHQLGYSLNPQDWRLLRSQLPNAGTSEDILMANVRCTLQDRDVTKCRAEYEFENTCSHENDVGLRCYEGAWAGVRFSMLAERADLQYVTVEKAGLFDYTTNAFKPAVQMDHARHNLENVRIVNNLQDGLGIIYADIYAGKSVNNIKNSEFSGNKGSGISLKQLDFRVSGSIIKDNKGSGVSHDAVISALDQKEIGGWFNMATDFNSFDTDYDPYLLPREISNIDLGTFEHKYIRTEELLGQNINRKIVVQCPAGYVIGIQLLNPIHNLSTESINILNARTENIRSDLWQVKRDLNVFPVTSSSYGIIIYYESGLQALGGAVLMLSTVTAPVQNIRNRIVSGAVPTLTIRSTKIQKNLRGITGIYYNRYIGDNGEYYLRKANESIKLINSELSYNEREAILIRSPFWDVISSNLSEVTLHVNGSLITQNGLGIRQMSKDLRSSNNLFHYVIQDTTFEQNTHGGFQVSLPYVWQYNENFTHSIYFGNSTWQRNRDFRISVSGHYTVFNITSNVFRENNCPGALISLDGMEKRLRFDNNRFESNNAKFVLLFKADSLSEIIGQVPASIEFNSFKGNNIVTMTANYRNHYMKVARRIRKQHKIPTAVIRLDGVQNVRLYRNLIAENEMDYNLVAGVRSARLNNYFEARENWWGTKDTAFIEAKIFDFDNWNDHADVIYQPFLIEDSYDASVSVVVPFNQDQEIDLTNYKGGRVYKDLLLTKQSTPYYISSDITVMPGKTLTINHGVTMEFEPNVGILVLGTLVAIGYRESPIVMRPFRNATRESLIDVQPKKRALEDMSAPLTEFDSIRLCTSANNCTGDADGLFGLNEGFLEYFNHTTLQWVPICDSRFTERNAQVVCRELGYDPLNVYYGHDRRIEFHTNSLTRIWSWVQPLECRGDEERMEDCAERLNGQLYGHRHECRWDDVFVFVSCNGIADDEVYWGGIRFANSKFEEIQYEHRLHNTRSHARLPLRESQLEFVRIEQAGILHNHKAAAIQAIHKNPSITSVSIENSANHGINMIAPSGKLNLNHLNINNTLGTGISIVSLSGEGRDSDESSFTPLKKLDLPYKLFSLVDICDPQKVLTIEERMLIYYKYDNNPVNCVKIFTSAFRAKPIGFRLLQSNLFNHSKLYGRTDFIKLYDGDIYNVTATYLGKIESDTDNQRSFFKTKGPTMSLQLVASGAPETHGFIAEVVTVPISTLGQYRDALHNITDTHISGAIKGAVTYSSAGEVTPTLTLIGNRIEKNCRQLYGNFSTCTSALNLDVQNMNSLYFMNNLITENQGGLRIRADSRGSATSLRGFVHHNLFMRNRNRPALYVEGRQSSPYQEVELYRNYFAQNMAGYEDVIRLCQVVSNFSYNYVHSNVGGRIMEVSGFEKVRLQIYQTTAHNGFYRNFATNWMTRATIVAGTAGQQYVDNIFENHENDYELLTVNNSILSFDYENRTFETWSSKIDARHNYWSYNNTISVQSRIRDKSDDPMLLEVLAVPFQMNNETILDGKCPPGWALVHDTCFIYVGAPMTFHEARDFCRSENSTMPFIRTDKTTLWKYLQSQMRHLKYPDKVWIQDYNHIDRCTSFVFGEIEIEDCNKERGFICESDPRVIIDPLSWRADIFAISIISAFVLAIILLILVAFCWFAKSKHRHTQRLQRRNSIRQSLRSLNSIDPQGSLRRRPNYNMSSNGTLSKGQDYKQMVANGSIDSMDKSVLSSEAGSFEGYEQKPHYNEYVNQNALRPAHPAQDHQSHKVATISKASGHRARAAAAAAAALEPDAFELSYRNEGFRDNSTYGDNTRANSISTSVAEDTPIIHHTDQEIDEGGSDYYGNASTLPLRTEGGTPAGRRGQPGLAFLSELKQNLPEYQRSSHSSFMPHRSSGDSLPFDQKLDQFNYSTESSLYRPAPAVPSSQQATPADMRRPDSYYTAVRSSKAPVSHYRTPRPLAQPPAAPNVAPAGGPAQRRPKTVYQTASEESSPTTPSPLTNQYHRSKSEALLETDFDGDGGSVGLQPLQTNGRSHSQPLETAM

Target ORF information:

RefSeq Version	NM_001258936.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bark beetle (bark), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258936.2

ORF Insert Sequence:

ATGAAGCTGC AACATCATAA AACCAACAGA CAGCGAATAA GTAAACCCCA CAGGGATCCA 
AAATGGGCCA GCATATGTTT ATGGCTGCTC GTAACGCTGG CTTTTTCGAC CCACTTGGCA 
CGGAGCCAAG AAAGTCGACA AACGGAGGAC TCCAAAGAGG TGGAACTGCT GCAGGATAAT 
GACATCGAGT TCGCCAGTCT CGATGGCGCC AGCCAACTAT TGCCAGCAAC CCGACATTCC 
GGCGCAGACG TCACCGTGGC GCCACAAGGT TCCACCCCAT CGATGACGTC ATCTTCGTCA 
TACACCGAGC TGCAAGGCGG CGAAATCCTC AGCGACCGAA TCCTGCGGCG CAGCGAGAGT 
CCTTATTTGG CACGCGACGA TATCGAGGTC CTGCGTGGAG CCCGTTTGAC CATCGAACCG 
GGTGTTACCA TCGAATTCGC CCCCACCAAA GGACTCAAAA TCAACGGCGT CCTGCAGGCG 
GTGGGCACAC CAACCTCCAG AATTGTGTTA AAATCCCAGA GTAACACGGC CAACTACAAA 
CTGGAGCTGC CCGATGATCA AGAGAAGGGC ATTCGACTAG TGGATGGACC CACTCCAGTG 
GAGGGAAGAC TGCAGCTTTT CCACAAGGGT GCATGGAGAT CGGTTTGCTC CAATTCCAGA 
AATTGGACAT TGGCGGATTA TGGAGTGGCT TGTAAGCAGC TGGGCTACAG AGGCGGCAGA 
TTCTGGAATT GGGTGGAGCG AACGCCAGGA TATTATCCCC GTTTGCTGTA CGAGCAGCCC 
AAGTGCAAAG GTGGCGAAGG AAGTCTTCAG GATTGCGCTT GGACCTCCCG GCAAATGGGA 
GCTGGTGCAT GTGATTATCA CAACGATTTG GGCATCCAAT GTCTGCCCGT CCATTCCGAA 
ACTTTGGGCC ATTGGCGAGG TATATACTTC GATAATGCGC CATCCACAAA GGCTCTGGGC 
AGGGATAATA TCGTTTATGC GGCCCAATCG GAGTCACGTC TGAAATATGT GGACATCATA 
AGGGCAGGAA GTGGTGCAGG ATTCAATGCC AAGTCGGCGG TGGAAGTCCA AGGATTACCT 
CCACAAATGG AGCACGTGGT GATCAGTCAC TCGGCCTACA CAGGATTCAA TAGCACACGT 
CCCTGGGCAG GATTTCAGCT ACAAAATGTC ACTGTGAGGA AATGCAATGG CATTGGAGTC 
TTCGTCAATT CGAGTCAAGG CGCTGTGCAA TTGGATGGAT GCTCCATAGT GGATAACGCT 
GGAGATGGTA TCAAATATGT GGGACACGAT TTGAGGGGAA CGGAAAGGAA GGATCGTGCC 
AGCATATATG ACTTTTGCAC TCTGCCCACA ACGTCGGGAC AAACCTATCC CATTTCGCTG 
TCCTTCACGC AGAAATATTA CGCTGGATCT GGCAAGGAGT GTGGCAAGTA CTTCTTCACA 
CGACCAGGAT ATCTGCTCAC TCTCCATTTT GAGAATTTCG TACTCATGCA AAACGAGACG 
GCCACAGTGG AAATTTACGA TGGAGCCTCG ACAAACGATC GCCTGCTGTT CGAATGGAAG 
GCCAGGAATT TTACGAGGCC CCAAAGTGTC ACCTCAACGA GGGAGAAAAT GTTTGTAAGG 
ATACGAGCGG ATGCCAGGCA GGAGCTAAAT GGTTTCTTCA GGATGACGTC CGGCGACTCG 
GTGGCCTACG ATCTTAAGGT GTCACAATCC ACGGTGGAGG ATAATGGAGG TCGTGGTGTT 
GCCATCGACA ATATAAGATC CAAGCTGCAT GTGCACAGTA GCTCCGTCTC CGGCAACGGT 
CATGTGGCAG GAGTTCATGT GACGAGTGGA GCTGGAGACG TGAACATAAC CAGCTCCAAC 
ATAAGCTTTA ACAATGGCGC TGGCGTTAAC ATCACCTATT ACGGTGGCAA TCGAAATATC 
TCCAGATCGG CATTGACTGC CAACAAGGGA TATGGCGTAG CCACTTGGCT AAATCAGACA 
TCGGACGTAA ATCGAATGGA GTACATACCT TTTAACCAAA CCAGCGTGGT GGAGTACTCG 
CAAATCGGTG GTAATCTGGA GACTGGAGTT TTTCACGGCA ACTTCTGTCG TCCCATTTGG 
GTAAACATCA CGGGAAACAG TTTCAACGGT AGTCAACAAA ATGATATATT CATTGAATCC 
TGCTACCAAG CCACTGCCAA TGGACGACCC AACATGCAAC TTCAGCTAGG ACATAATCAG 
TTTAAATACT CCCAAGCGAA CTCCATCTAT CTGAGTCCAG CTTTGAATCT TCAAGGACGC 
ATCGAATACA ATATGTTCCG CTTTGGTTCC TATGGTTGTC TGTTCATCAA CAACGACTAC 
ATATATCCAG AGTTTAATTA TTTCCCGGTC AAGCTGATCA TACAGAGTAA CTACTTCATG 
AGAAACAGCG GAGTTCATGT GGTTTCTCTG GGCCTATCGC CCTACAGCAG AGCGGAAGTA 
CAGTATATAC TCTTCACGAG AAACTTTGTG AGAGGAAACA ATATAACGGA GGCTTTTGGT 
CCTCTAATCG CAGGCTCAGA GGGCAGTGAT GGTGCTGGCC GACTAAATCC GCGATCCCGA 
GTTGCAGCCC CTGTTGTCGT GGGATCAAGT AATGTTGATA TATTCCGAAA CATCCTGCAC 
AATCTGGATT CCATGTATGA AATTGGCTCA CAACTCACTG ATCAAAGTAA GATCATCAAC 
GCCACTTGCA ATTGGCTGGG TCACACGGAT GAGAATAAGA TATACGCTCG GCTGTTTCAT 
CGCAACGATC GCTATAATTT GGCGAAAATA AACTATCTAC CATACCTGTT GCACAGCTCC 
AATCCAGGTT CCACTGCCAT GATTACGGTT TCCACTGTGG TTCCACGATT TTTCCACGAG 
GGAAGCGATG TCATTGGTGG CGAAGTCGAT GGTCAGGATA TGGTACCAGC TGGTACATAT 
ACCGTAACCA AGGACATCAA TATCAGACCT GGTGGTAAGC TCATACTCCA ACCTGGTACC 
ACTCTCAAGT TCGAACCCAG TGTGGGAATG ATGGTGGCTG GAAAATTGGA AGCCAGAGGA 
CGTAGACCCG ATGACATACT CTTCACTTTG AAGCGGGAAA CAATTATGGG CGAATCAAAT 
GATACGGAAA CCATCGATTT GGATAGCGAA ACTGAGGCCA TAGATATGGA AACGGAAGTA 
ATACCCGCGG ATGGTGTACC TCGAGTTCCA GTTCGCTTGG TCGGAGGAGC GGGTGCCAAT 
GAGGGAAGAC TTCAGGTATA CCTAAAGGGA CGATGGGGCA CTGTATGTGA TTATGGCTGG 
AATGTCCTGA ACGCAGCTCT GGTTTGCCAC CAATTGGGTT ACTCCCTGAA TCCACAGGAT 
TGGAGACTAC TACGCTCGCA GTTACCAAAT GCTGGCACAT CCGAGGACAT TTTAATGGCC 
AATGTCAGGT GTACACTCCA GGATCGAGAC GTGACCAAGT GCCGGGCTGA ATACGAGTTC 
GAAAACACGT GTAGCCATGA AAACGATGTG GGTCTCAGGT GTTATGAGGG TGCCTGGGCT 
GGTGTGCGTT TCAGTATGTT GGCCGAAAGA GCGGATCTAC AGTATGTAAC TGTGGAAAAG 
GCCGGACTTT TCGATTATAC CACCAATGCA TTTAAGCCCG CTGTTCAGAT GGATCATGCG 
CGTCACAATC TGGAAAACGT AAGAATCGTG AATAATCTAC AAGACGGTTT GGGTATTATA 
TACGCAGACA TATATGCTGG CAAGTCTGTG AACAACATCA AAAACTCGGA GTTCTCTGGC 
AATAAGGGAA GTGGAATTAG TCTAAAACAA TTGGACTTCA GGGTCTCAGG ATCCATTATC 
AAGGACAACA AAGGCAGTGG TGTTTCTCAC GATGCGGTGA TATCTGCTTT GGATCAAAAG 
GAAATCGGGG GATGGTTTAA CATGGCTACA GATTTCAATT CTTTCGATAC TGACTACGAT 
CCTTATCTCT TGCCCCGTGA AATAAGCAAC ATCGACTTGG GAACCTTCGA ACACAAGTAC 
ATAAGAACTG AAGAGCTATT GGGACAAAAT ATTAATCGCA AAATAGTGGT TCAATGTCCG 
GCAGGCTATG TTATTGGAAT ACAGCTCTTG AATCCCATAC ACAATCTGTC CACAGAGAGC 
ATAAACATCC TGAATGCCAG GACAGAGAAT ATTAGAAGTG ATCTGTGGCA AGTTAAGCGA 
GATTTAAACG TATTCCCAGT AACCAGCAGC AGTTATGGCA TTATAATTTA CTACGAAAGC 
GGACTGCAGG CCTTGGGAGG AGCTGTTTTG ATGCTGAGCA CAGTCACAGC TCCAGTGCAA 
AACATAAGGA ATCGCATTGT GAGCGGTGCA GTGCCCACTC TAACCATTCG ATCAACGAAG 
ATACAAAAGA ATTTGAGGGG TATTACGGGC ATCTACTACA ATCGCTACAT AGGCGACAAT 
GGGGAGTATT ATTTGAGAAA AGCCAACGAA TCAATTAAGC TAATCAACTC TGAGCTTAGC 
TACAATGAAC GAGAAGCTAT ACTTATAAGG TCACCATTCT GGGATGTGAT ATCCAGTAAC 
CTATCAGAGG TTACACTTCA TGTCAATGGT TCGCTAATCA CTCAAAATGG ACTTGGAATA 
CGACAAATGT CGAAGGATCT GCGATCGTCG AACAACCTAT TCCATTACGT CATACAGGAT 
ACTACATTTG AGCAGAATAC TCATGGAGGC TTTCAGGTAT CTTTGCCATA CGTGTGGCAA 
TACAACGAAA ACTTCACGCA TTCGATTTAT TTCGGCAACA GCACCTGGCA AAGAAATCGC 
GACTTCCGCA TTTCCGTTTC CGGTCACTAT ACGGTCTTCA ATATAACCTC AAATGTCTTT 
AGAGAGAATA ACTGCCCTGG CGCACTGATA TCTCTAGATG GAATGGAAAA GCGACTTCGT 
TTTGATAACA ATCGTTTTGA GTCGAATAAC GCCAAGTTTG TGCTGCTTTT CAAGGCGGAT 
TCTCTAAGTG AAATCATTGG ACAAGTACCT GCCAGCATTG AATTTAACAG CTTTAAAGGA 
AATAATATTG TTACCATGAC GGCAAACTAT AGGAATCATT ATATGAAAGT GGCCAGAAGG 
ATACGAAAAC AACACAAAAT ACCCACCGCA GTGATTCGAC TGGATGGTGT CCAGAATGTG 
CGACTATATC GCAATCTGAT AGCCGAAAAT GAAATGGACT ATAATTTGGT GGCAGGTGTG 
AGATCGGCCA GATTGAATAA CTACTTTGAG GCAAGAGAGA ACTGGTGGGG CACCAAGGAT 
ACCGCCTTCA TCGAGGCTAA GATTTTCGAC TTTGACAACT GGAACGATCA TGCGGATGTC 
ATATATCAGC CATTCCTGAT CGAAGATAGT TACGATGCCA GTGTTTCTGT GGTCGTACCC 
TTCAATCAAG ATCAAGAGAT AGATTTGACC AACTATAAAG GTGGTAGAGT GTATAAGGAC 
TTGTTATTGA CGAAACAGAG TACTCCCTAC TATATATCCT CTGATATCAC GGTTATGCCA 
GGCAAAACTT TGACTATAAA CCATGGCGTC ACAATGGAAT TTGAACCAAA CGTTGGAATA 
TTGGTTTTGG GCACACTAGT GGCCATTGGA TACAGAGAAT CACCTATCGT GATGAGGCCC 
TTTAGAAATG CCACCAGGGA ATCTCTGATC GATGTTCAGC CCAAGAAACG GGCACTGGAG 
GATATGAGTG CTCCGCTCAC CGAATTCGAT TCCATAAGAC TGTGCACCAG TGCCAACAAT 
TGCACAGGTG ATGCCGACGG ATTGTTCGGT TTGAATGAGG GCTTTTTGGA GTACTTCAAT 
CACACCACTC TTCAGTGGGT TCCCATTTGT GACAGCAGAT TTACAGAACG AAATGCTCAA 
GTTGTTTGCC GCGAACTGGG CTATGATCCT CTGAATGTTT ACTATGGTCA TGATCGCAGG 
ATAGAGTTCC ACACGAACTC ACTGACGCGA ATTTGGTCAT GGGTGCAGCC ATTGGAATGT 
CGAGGTGACG AGGAGAGAAT GGAGGATTGT GCGGAGCGAT TGAATGGTCA ACTTTATGGA 
CATCGCCACG AATGCCGTTG GGATGATGTA TTCGTCTTTG TCAGCTGCAA TGGCATTGCT 
GACGATGAAG TTTATTGGGG TGGCATAAGA TTCGCCAACT CCAAGTTCGA GGAAATCCAA 
TATGAACATC GTTTGCACAA CACAAGATCA CATGCGCGGT TGCCATTGAG GGAAAGTCAA 
TTGGAGTTCG TCAGGATTGA ACAGGCAGGC ATACTGCATA ATCACAAAGC CGCTGCCATT 
CAAGCCATTC ACAAGAACCC ATCCATAACG TCGGTGAGCA TAGAAAACTC TGCTAATCAT 
GGCATAAACA TGATTGCACC CAGTGGTAAA CTGAATTTGA ATCACCTGAA CATTAACAAT 
ACTTTGGGTA CCGGAATTAG CATTGTATCG CTGAGTGGCG AGGGTCGTGA TAGCGATGAA 
TCTAGTTTTA CGCCCTTAAA GAAGTTGGAT CTCCCATATA AGCTCTTCTC CCTGGTGGAC 
ATATGTGATC CGCAAAAGGT GCTGACCATC GAAGAACGCA TGCTAATTTA CTACAAATAT 
GATAACAATC CAGTGAACTG TGTGAAGATC TTTACCTCAG CTTTTCGAGC TAAGCCAATT 
GGTTTCAGAT TGTTGCAATC GAATTTGTTC AATCACTCCA AATTGTATGG CAGAACCGAT 
TTTATCAAGT TGTACGACGG TGATATCTAT AATGTGACTG CCACTTATTT GGGTAAAATC 
GAATCCGATA CGGATAATCA AAGATCGTTC TTCAAGACCA AGGGCCCAAC GATGAGTCTC 
CAGTTGGTGG CTAGTGGTGC TCCCGAAACA CATGGTTTCA TCGCCGAGGT CGTCACTGTG 
CCCATTTCCA CTTTGGGTCA ATATCGCGAT GCCTTGCACA ATATCACAGA TACTCATATC 
TCAGGTGCGA TCAAAGGAGC GGTTACATAC TCATCCGCCG GTGAGGTCAC GCCCACGTTG 
ACTTTAATTG GCAATCGGAT AGAAAAGAAT TGCCGACAAT TGTATGGCAA TTTCTCCACC 
TGCACTTCAG CTCTGAATTT GGATGTGCAG AATATGAATT CGTTGTATTT TATGAACAAC 
CTGATAACAG AAAATCAAGG TGGTCTTCGG ATTAGAGCTG ATTCTCGAGG TTCAGCCACC 
TCACTTCGAG GCTTTGTTCA TCACAATCTC TTCATGAGGA ATCGCAATCG TCCAGCTCTG 
TACGTCGAAG GCAGACAATC CTCGCCGTAT CAAGAAGTGG AACTTTATCG CAACTACTTT 
GCCCAGAATA TGGCCGGTTA TGAGGATGTA ATAAGATTGT GCCAAGTGGT ATCCAACTTT 
AGCTATAACT ATGTGCACAG CAATGTGGGA GGAAGAATCA TGGAGGTATC TGGCTTCGAA 
AAGGTGCGAT TGCAGATCTA TCAGACAACG GCTCACAATG GTTTCTACAG GAATTTTGCC 
ACCAACTGGA TGACCAGGGC AACAATTGTG GCCGGAACCG CGGGCCAGCA ATATGTTGAT 
AATATTTTCG AGAATCACGA AAACGACTAT GAGCTGCTCA CAGTCAACAA TTCCATTTTG 
AGTTTTGACT ATGAAAACAG GACCTTTGAA ACCTGGAGCT CTAAGATCGA CGCTCGTCAC 
AATTATTGGA GCTATAATAA TACCATCTCG GTGCAATCTC GTATTAGAGA TAAGTCAGAT 
GATCCTATGC TACTGGAGGT CTTAGCCGTG CCCTTCCAAA TGAACAATGA GACAATACTG 
GATGGTAAAT GTCCTCCAGG TTGGGCACTG GTCCATGACA CCTGTTTTAT CTACGTGGGA 
GCTCCCATGA CTTTCCACGA AGCTCGAGAT TTCTGTCGCT CGGAGAACTC CACAATGCCC 
TTCATACGCA CCGATAAGAC GACGCTATGG AAGTATTTGC AGAGCCAGAT GCGACACTTG 
AAATATCCGG ATAAGGTTTG GATTCAGGAC TATAATCACA TAGATCGCTG CACGAGCTTT 
GTGTTTGGGG AAATCGAAAT TGAGGATTGC AACAAGGAGC GCGGTTTTAT TTGCGAATCG 
GATCCCAGGG TTATCATTGA TCCCCTATCC TGGCGTGCAG ACATCTTTGC CATCAGTATT 
ATCTCAGCCT TTGTCCTTGC CATTATATTG CTGATCCTGG TGGCCTTCTG TTGGTTCGCT 
AAATCAAAAC ATCGACATAC CCAACGACTC CAGAGAAGAA ACAGCATTCG CCAAAGTCTG 
CGCAGCTTGA ACAGCATCGA TCCACAGGGT TCCCTTCGCA GAAGACCCAA TTATAATATG 
TCCAGCAATG GAACATTGTC CAAGGGTCAG GACTACAAGC AGATGGTGGC CAATGGTTCC 
ATAGATTCCA TGGACAAAAG TGTATTAAGC TCGGAAGCTG GTAGCTTTGA GGGCTATGAA 
CAGAAGCCCC ACTACAATGA GTATGTGAAT CAGAATGCCT TGCGACCAGC ACATCCTGCA 
CAGGATCATC AGAGTCACAA GGTGGCCACC ATTTCCAAGG CGAGTGGCCA TAGAGCGAGA 
GCCGCTGCAG CTGCTGCTGC GGCCTTGGAG CCCGACGCCT TTGAGTTAAG CTACCGAAAC 
GAAGGATTCC GGGATAACTC CACCTACGGT GATAACACCC GAGCCAATTC CATTAGTACC 
AGTGTGGCAG AGGACACGCC AATCATACAT CACACAGATC AGGAGATCGA TGAGGGTGGT 
TCCGATTATT ATGGAAATGC GAGCACCTTG CCACTTCGAA CCGAGGGAGG AACTCCGGCT 
GGAAGGCGTG GCCAACCCGG CTTGGCCTTT CTCTCCGAAC TCAAACAGAA CCTGCCCGAG 
TACCAAAGAA GCTCGCATAG CAGTTTTATG CCCCACCGAA GCAGCGGCGA CTCACTGCCC 
TTTGATCAGA AGCTAGATCA ATTCAACTAC TCTACAGAGT CCTCACTGTA CCGCCCAGCG 
CCTGCGGTTC CCAGTAGTCA GCAAGCAACA CCGGCGGACA TGCGACGTCC GGACTCGTAT 
TATACTGCCG TCAGGAGTAG TAAAGCTCCA GTATCACATT ACCGGACACC GAGGCCACTG 
GCACAGCCAC CGGCTGCACC AAATGTGGCG CCCGCGGGTG GACCGGCGCA ACGGCGTCCC 
AAGACGGTTT ACCAAACCGC ATCCGAGGAG TCGTCGCCAA CGACACCGTC ACCACTGACC 
AATCAGTACC ACCGCTCCAA ATCGGAGGCT CTACTCGAAA CAGATTTCGA TGGGGATGGA 
GGATCGGTTG GTCTGCAGCC ACTGCAAACA AACGGACGAA GTCACAGTCA GCCCCTGGAA 
ACGGCCATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bark cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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