side-II cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	side-II
Entrez Gene ID	3346206
Full Name	CG42313 gene product from transcript CG42313-RB
Synonyms	42313,BG:DS01219.3,BG:DS03144.1,BG:SD01219.3,CG15275,CG15276,CG15277,CG33515,CT35222,Dmel\CG42313,Dmel_CG15277,Dmel_CG33515,cg15277
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B7-35B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014485.5	NP_001014485.3	sidestep II, isoform A
NM_001299017.1	NP_001285946.1	sidestep II, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG42313	NP_001014485.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009683	XP_318742.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following side-II gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the side-II cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15539	NM_001299017.1 Latest version!	Drosophila melanogaster sidestep II (side-II), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15539	NM_001014485.5 Latest version!	Drosophila melanogaster sidestep II (side-II), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

side-II ( NM_001014485.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014485.3 Drosophila melanogaster sidestep II (side-II), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15539
Clone ID Related Accession (Same CDS sequence)	NM_001014485.5 , NM_001299017.1
Accession Version	NM_001014485.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep II, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001014485.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCTGC TTCTGGCCAT GGCACTGCTA ATGCCGGTCA CATTAGCGCG CCTGCATTCC 
ACTTCCGGCG TAGGAATGTC CGGCGGAGGC AGCAGCATGG GTCCTGGCGG AGGAGGATCC 
GGCAGCGGAG GCATATCCTC GAAGGATTCA CACGTGAAAA CGAAGGACAT TGATGCCGTC 
GAGGGAAAAT CGGTGTCGCT GCCCTGTCCC ATCACAGAGC CACTGGATAA TGTGTATATG 
GTGCTCTGGT TCCGCGATAA TGCCGGCATA CCGCTCTATA GCTTCGATGT GCGCGATAAG 
GAGAGTCGGG AGCAGCCACG ACATTGGTCA GCCCCGGAGG TTTTTGGCTC ACGTGCCAAA 
TTCCATTTTG ACTCGCAGCC AGCAACCTTG GAAATTAAGC GACATGATCA GGGTATTTAC 
CGTTGTCGTG TGGATTTTCG CACCAGTCAA ACGCAAAGTT TCCGTTTCAA TTTGTCAGTA 
ATAATACTTC CGGAGCAGCC AATCATCATG GACCGTTGGG GTCGTCAGCT GAATGGTACT 
CAATTGGGGC CCAAGCAGGA GGGCGATGAT ATCGTTATCA CTTGTCGTGT GGTGGGCGGT 
CGGCCGCAAC CGCAAGTCCG TTGGCTCGTA AATGGACTGC TTGTCGACAA TCAAAACGAG 
CACAATTCCG GCGATGTTAT CGAGAACCGT CTGTTGTGGC CATCGGTGCA GCGCAACGAT 
TTGAATTCCG TATTCACATG TCAGGCACTG AATACGCAAT TAGATAAACC CAAGGAGAAG 
AGCTTCATAC TGGATATGCA CTTGAAGCCT TTGGTTGTGA AAATCCTGGA GCCGCCCAGC 
TCGATGATTG CCGATCGCCG CTACGAGGTG TCCTGCGAAT CCTCCGGCTC TCGGCCAAAT 
GCCATCATCA CCTGGTACAA GGGAAAACGG CAATTACGAC GCACAAAGGA CGACATATCA 
AAGAACTCGA CACGCTCCGA GCTGAGCTTT GTGCCGACCA CAGACGACGA TGGCAAATCG 
ATAACATGCC GTGCGGAAAA TCCAAATGTG AATGGCCTGT ATTTGGAGAC CATGTGGAAA 
TTGAATGTCG TTTATCCTCC CTTGGTGACG TTACGCTTGG GCTCAACTTT GACACCGGAT 
GATATCAAGG AAGGCGACGA TGTTTACTTC GAGTGTCACG TACAATCGAA TCCTCAATGG 
CGGAAACTGT TGTGGTTGCA TAATGGTATT CATCTGGAGC ACAATACATC CGCCCGCGTC 
ATTCGCTCGA ATCAGAGCCT GGTACTCCAG AAGATTACGA AACACTACGC CGGGAACTAT 
GCGTGCAGTG CTATAAACGA CGAGGGCGAG ACGGTCAGCA ATCAGTTGCC ACTGCGGGTG 
AAATACACGC CCATGTGCAA GCACGCCGAC CGCGTAATAT TAATTGGCGC CTCTAAGGAC 
GAGACTGTTG AGGTTGTGTG CGAAATTCAA GCCGATCCGC CGCCGCGGAC TTTTCGCTGG 
AAATTCAACA ACTCGGGCGA AACTCTGGAC GTGGGCAGTG AACGATTCAG CGTGAACGGC 
AGCCGCAGCA TTTTAAAGTA CACACCAGTC ACAGATCAGG ATTATGGCAC ACTGTCATGC 
TGGGCCTCCA ACGAGGTGGG CACCCAGCAG CACCCCTGTC TCTTCCAAGT CGTGCTAGCC 
GCACTGCCGA ATGCCGTCAG CAATTGTACC GTCTTCAACA GAACTGAACT GAGTGTTGAC 
ATACAGTGCA TACCGGGCTA TGACGGCGGC CTGCCGCAAA TATTTGTACT CGAAATGTTT 
TCAACACGCA CCGGCATCAC CAGATTCAAT TTGAGCAACG CCGAGGAGGC ACTATTTTCG 
CTGGAGAACC TGGACACTCT GACCTCGATG ATGGTCCAGG AGAATAATTC GCTGCGGCTG 
CGCATCTACT CGTATAACCA GAAGGGCCGC AGTGCCGCTT ATCTGCTAAC CGATTTCATA 
ATTGGCTCAA CAGCTTACAA GACAGACGAC GATGTAACAC TGACATTGTC GCCGGTGATT 
GTCGGCAGTG TCCTGACCAT CATAATTATT GGTGTGGCCA TCGCGATACG AATATTCGTG 
GTGACATTTG TGCACAATTT GCGGCGGAAT CGTCATCATG GCAGCAAGGC CACCGCCGCC 
GGAGTAACCG CCCAGCCCAG CGATGTCTTC AAGGGCGGCA ACTTGGAGAA GCCGCGATGG 
CAGCACACGG ACATAAAAAC GAAATCATCA GAGGATTTGG TTGAGGACGA ACGTGATCCG 
GATGTAATAC CATCGCAATA CGTCGGTGGG AGCAGCGCCG GCAGCAGTGG CAGCAATGCC 
TCCAACGTGG GCAGCACCAC CGCTGGCACC GCCACCGGGA GCAGCACCTC CACGGCGGTG 
GTGGCCAACG CCAATGCGGC CGCGTCAGCG ATGGCTGGAC CGGGAGGAGC CACATCGTCA 
GCATCTGCCA CAGATGGACA TCAGTTTGCA TCTGGCACAG CGGCGGCAGG AGGCGGGGCC 
TCCAAGTGGT CACCCAATGG CAATGTGACG ACGACGATGC TGACCGGCGA TGCGGTGAAT 
GCAACTGTCA CCTACAATCA AATCAACGCG CAACGGGCGC AGCTGTTGGC GGCGGTGGCT 
GCAGTGGGCG GTGGATGCAG CAGCACCGTA ATCTCGCCCA GCAGCAGCAT ACTGGGCGGT 
CTTGGCAAGC CGCTGGGCAT GGGAATGGGC ATGGGCCTGG GCATGGGAGT GGGTGGCACG 
GTCATCGGGT CGCCCACGTC GCTATCGTCG ACGGCCACTT TGGCAGCTCA CCTGCAGCCG 
GCGCACAGTA GCGGAGTGGG CACATTGCCA CCGGGACTCG GAAGCGGCGG TGGCTACATA 
CTGAACGCTT ATGCCAGCGG GCGCCTGCAT CATCCACCTG GGGGTGGGGT GCCAGGCGGA 
GCGACTCACA CCGCCACACT GAACAGGCAT CACCACCAGC AGCAGCAGCT CCACCACCAC 
CCGCACCACC AGCAGCCACA CCACTCCGGC TCAAGTCTTT TGATGGGAAG TGCTGGAAGC 
GTGATGGGCG TTGTGGGATC CACCACGACA GCCACAACCT CGTGTAACTC CTCGCAGGAT 
CTGGACGATA ACATCAATAT AAATGCGATT AAGGACTGCC TCATGACGCA GCGAGTGCCC 
GAGAGTTGTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014485.3
CDS	1072..4266
Translation	MVLLLAMALLMPVTLARLHSTSGVGMSGGGSSMGPGGGGSGSGGISSKDSHVKTKDIDAVEGKSVSLPCPITEPLDNVYMVLWFRDNAGIPLYSFDVRDKESREQPRHWSAPEVFGSRAKFHFDSQPATLEIKRHDQGIYRCRVDFRTSQTQSFRFNLSVIILPEQPIIMDRWGRQLNGTQLGPKQEGDDIVITCRVVGGRPQPQVRWLVNGLLVDNQNEHNSGDVIENRLLWPSVQRNDLNSVFTCQALNTQLDKPKEKSFILDMHLKPLVVKILEPPSSMIADRRYEVSCESSGSRPNAIITWYKGKRQLRRTKDDISKNSTRSELSFVPTTDDDGKSITCRAENPNVNGLYLETMWKLNVVYPPLVTLRLGSTLTPDDIKEGDDVYFECHVQSNPQWRKLLWLHNGIHLEHNTSARVIRSNQSLVLQKITKHYAGNYACSAINDEGETVSNQLPLRVKYTPMCKHADRVILIGASKDETVEVVCEIQADPPPRTFRWKFNNSGETLDVGSERFSVNGSRSILKYTPVTDQDYGTLSCWASNEVGTQQHPCLFQVVLAALPNAVSNCTVFNRTELSVDIQCIPGYDGGLPQIFVLEMFSTRTGITRFNLSNAEEALFSLENLDTLTSMMVQENNSLRLRIYSYNQKGRSAAYLLTDFIIGSTAYKTDDDVTLTLSPVIVGSVLTIIIIGVAIAIRIFVVTFVHNLRRNRHHGSKATAAGVTAQPSDVFKGGNLEKPRWQHTDIKTKSSEDLVEDERDPDVIPSQYVGGSSAGSSGSNASNVGSTTAGTATGSSTSTAVVANANAAASAMAGPGGATSSASATDGHQFASGTAAAGGGASKWSPNGNVTTTMLTGDAVNATVTYNQINAQRAQLLAAVAAVGGGCSSTVISPSSSILGGLGKPLGMGMGMGLGMGVGGTVIGSPTSLSSTATLAAHLQPAHSSGVGTLPPGLGSGGGYILNAYASGRLHHPPGGGVPGGATHTATLNRHHHQQQQLHHHPHHQQPHHSGSSLLMGSAGSVMGVVGSTTTATTSCNSSQDLDDNININAIKDCLMTQRVPESCV

Target ORF information:

RefSeq Version	NM_001014485.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep II (side-II), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014485.5

ORF Insert Sequence:

ATGGTCCTGC TTCTGGCCAT GGCACTGCTA ATGCCGGTCA CATTAGCGCG CCTGCATTCC 
ACTTCCGGCG TAGGAATGTC CGGCGGAGGC AGCAGCATGG GTCCTGGCGG AGGAGGATCC 
GGCAGCGGAG GCATATCCTC GAAGGATTCA CACGTGAAAA CGAAGGACAT TGATGCCGTC 
GAGGGAAAAT CGGTGTCGCT GCCCTGTCCC ATCACAGAGC CACTGGATAA TGTGTATATG 
GTGCTCTGGT TCCGCGATAA TGCCGGCATA CCGCTCTATA GCTTCGATGT GCGCGATAAG 
GAGAGTCGGG AGCAGCCACG ACATTGGTCA GCCCCGGAGG TTTTTGGCTC ACGTGCCAAA 
TTCCATTTTG ACTCGCAGCC AGCAACCTTG GAAATTAAGC GACATGATCA GGGTATTTAC 
CGTTGTCGTG TGGATTTTCG CACCAGTCAA ACGCAAAGTT TCCGTTTCAA TTTGTCAGTA 
ATAATACTTC CGGAGCAGCC AATCATCATG GACCGTTGGG GTCGTCAGCT GAATGGTACT 
CAATTGGGGC CCAAGCAGGA GGGCGATGAT ATCGTTATCA CTTGTCGTGT GGTGGGCGGT 
CGGCCGCAAC CGCAAGTCCG TTGGCTCGTA AATGGACTGC TTGTCGACAA TCAAAACGAG 
CACAATTCCG GCGATGTTAT CGAGAACCGT CTGTTGTGGC CATCGGTGCA GCGCAACGAT 
TTGAATTCCG TATTCACATG TCAGGCACTG AATACGCAAT TAGATAAACC CAAGGAGAAG 
AGCTTCATAC TGGATATGCA CTTGAAGCCT TTGGTTGTGA AAATCCTGGA GCCGCCCAGC 
TCGATGATTG CCGATCGCCG CTACGAGGTG TCCTGCGAAT CCTCCGGCTC TCGGCCAAAT 
GCCATCATCA CCTGGTACAA GGGAAAACGG CAATTACGAC GCACAAAGGA CGACATATCA 
AAGAACTCGA CACGCTCCGA GCTGAGCTTT GTGCCGACCA CAGACGACGA TGGCAAATCG 
ATAACATGCC GTGCGGAAAA TCCAAATGTG AATGGCCTGT ATTTGGAGAC CATGTGGAAA 
TTGAATGTCG TTTATCCTCC CTTGGTGACG TTACGCTTGG GCTCAACTTT GACACCGGAT 
GATATCAAGG AAGGCGACGA TGTTTACTTC GAGTGTCACG TACAATCGAA TCCTCAATGG 
CGGAAACTGT TGTGGTTGCA TAATGGTATT CATCTGGAGC ACAATACATC CGCCCGCGTC 
ATTCGCTCGA ATCAGAGCCT GGTACTCCAG AAGATTACGA AACACTACGC CGGGAACTAT 
GCGTGCAGTG CTATAAACGA CGAGGGCGAG ACGGTCAGCA ATCAGTTGCC ACTGCGGGTG 
AAATACACGC CCATGTGCAA GCACGCCGAC CGCGTAATAT TAATTGGCGC CTCTAAGGAC 
GAGACTGTTG AGGTTGTGTG CGAAATTCAA GCCGATCCGC CGCCGCGGAC TTTTCGCTGG 
AAATTCAACA ACTCGGGCGA AACTCTGGAC GTGGGCAGTG AACGATTCAG CGTGAACGGC 
AGCCGCAGCA TTTTAAAGTA CACACCAGTC ACAGATCAGG ATTATGGCAC ACTGTCATGC 
TGGGCCTCCA ACGAGGTGGG CACCCAGCAG CACCCCTGTC TCTTCCAAGT CGTGCTAGCC 
GCACTGCCGA ATGCCGTCAG CAATTGTACC GTCTTCAACA GAACTGAACT GAGTGTTGAC 
ATACAGTGCA TACCGGGCTA TGACGGCGGC CTGCCGCAAA TATTTGTACT CGAAATGTTT 
TCAACACGCA CCGGCATCAC CAGATTCAAT TTGAGCAACG CCGAGGAGGC ACTATTTTCG 
CTGGAGAACC TGGACACTCT GACCTCGATG ATGGTCCAGG AGAATAATTC GCTGCGGCTG 
CGCATCTACT CGTATAACCA GAAGGGCCGC AGTGCCGCTT ATCTGCTAAC CGATTTCATA 
ATTGGCTCAA CAGCTTACAA GACAGACGAC GATGTAACAC TGACATTGTC GCCGGTGATT 
GTCGGCAGTG TCCTGACCAT CATAATTATT GGTGTGGCCA TCGCGATACG AATATTCGTG 
GTGACATTTG TGCACAATTT GCGGCGGAAT CGTCATCATG GCAGCAAGGC CACCGCCGCC 
GGAGTAACCG CCCAGCCCAG CGATGTCTTC AAGGGCGGCA ACTTGGAGAA GCCGCGATGG 
CAGCACACGG ACATAAAAAC GAAATCATCA GAGGATTTGG TTGAGGACGA ACGTGATCCG 
GATGTAATAC CATCGCAATA CGTCGGTGGG AGCAGCGCCG GCAGCAGTGG CAGCAATGCC 
TCCAACGTGG GCAGCACCAC CGCTGGCACC GCCACCGGGA GCAGCACCTC CACGGCGGTG 
GTGGCCAACG CCAATGCGGC CGCGTCAGCG ATGGCTGGAC CGGGAGGAGC CACATCGTCA 
GCATCTGCCA CAGATGGACA TCAGTTTGCA TCTGGCACAG CGGCGGCAGG AGGCGGGGCC 
TCCAAGTGGT CACCCAATGG CAATGTGACG ACGACGATGC TGACCGGCGA TGCGGTGAAT 
GCAACTGTCA CCTACAATCA AATCAACGCG CAACGGGCGC AGCTGTTGGC GGCGGTGGCT 
GCAGTGGGCG GTGGATGCAG CAGCACCGTA ATCTCGCCCA GCAGCAGCAT ACTGGGCGGT 
CTTGGCAAGC CGCTGGGCAT GGGAATGGGC ATGGGCCTGG GCATGGGAGT GGGTGGCACG 
GTCATCGGGT CGCCCACGTC GCTATCGTCG ACGGCCACTT TGGCAGCTCA CCTGCAGCCG 
GCGCACAGTA GCGGAGTGGG CACATTGCCA CCGGGACTCG GAAGCGGCGG TGGCTACATA 
CTGAACGCTT ATGCCAGCGG GCGCCTGCAT CATCCACCTG GGGGTGGGGT GCCAGGCGGA 
GCGACTCACA CCGCCACACT GAACAGGCAT CACCACCAGC AGCAGCAGCT CCACCACCAC 
CCGCACCACC AGCAGCCACA CCACTCCGGC TCAAGTCTTT TGATGGGAAG TGCTGGAAGC 
GTGATGGGCG TTGTGGGATC CACCACGACA GCCACAACCT CGTGTAACTC CTCGCAGGAT 
CTGGACGATA ACATCAATAT AAATGCGATT AAGGACTGCC TCATGACGCA GCGAGTGCCC 
GAGAGTTGTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

side-II ( NM_001299017.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285946.1 Drosophila melanogaster sidestep II (side-II), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15539
Clone ID Related Accession (Same CDS sequence)	NM_001014485.5 , NM_001299017.1
Accession Version	NM_001299017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sidestep II, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCCTGC TTCTGGCCAT GGCACTGCTA ATGCCGGTCA CATTAGCGCG CCTGCATTCC 
ACTTCCGGCG TAGGAATGTC CGGCGGAGGC AGCAGCATGG GTCCTGGCGG AGGAGGATCC 
GGCAGCGGAG GCATATCCTC GAAGGATTCA CACGTGAAAA CGAAGGACAT TGATGCCGTC 
GAGGGAAAAT CGGTGTCGCT GCCCTGTCCC ATCACAGAGC CACTGGATAA TGTGTATATG 
GTGCTCTGGT TCCGCGATAA TGCCGGCATA CCGCTCTATA GCTTCGATGT GCGCGATAAG 
GAGAGTCGGG AGCAGCCACG ACATTGGTCA GCCCCGGAGG TTTTTGGCTC ACGTGCCAAA 
TTCCATTTTG ACTCGCAGCC AGCAACCTTG GAAATTAAGC GACATGATCA GGGTATTTAC 
CGTTGTCGTG TGGATTTTCG CACCAGTCAA ACGCAAAGTT TCCGTTTCAA TTTGTCAGTA 
ATAATACTTC CGGAGCAGCC AATCATCATG GACCGTTGGG GTCGTCAGCT GAATGGTACT 
CAATTGGGGC CCAAGCAGGA GGGCGATGAT ATCGTTATCA CTTGTCGTGT GGTGGGCGGT 
CGGCCGCAAC CGCAAGTCCG TTGGCTCGTA AATGGACTGC TTGTCGACAA TCAAAACGAG 
CACAATTCCG GCGATGTTAT CGAGAACCGT CTGTTGTGGC CATCGGTGCA GCGCAACGAT 
TTGAATTCCG TATTCACATG TCAGGCACTG AATACGCAAT TAGATAAACC CAAGGAGAAG 
AGCTTCATAC TGGATATGCA CTTGAAGCCT TTGGTTGTGA AAATCCTGGA GCCGCCCAGC 
TCGATGATTG CCGATCGCCG CTACGAGGTG TCCTGCGAAT CCTCCGGCTC TCGGCCAAAT 
GCCATCATCA CCTGGTACAA GGGAAAACGG CAATTACGAC GCACAAAGGA CGACATATCA 
AAGAACTCGA CACGCTCCGA GCTGAGCTTT GTGCCGACCA CAGACGACGA TGGCAAATCG 
ATAACATGCC GTGCGGAAAA TCCAAATGTG AATGGCCTGT ATTTGGAGAC CATGTGGAAA 
TTGAATGTCG TTTATCCTCC CTTGGTGACG TTACGCTTGG GCTCAACTTT GACACCGGAT 
GATATCAAGG AAGGCGACGA TGTTTACTTC GAGTGTCACG TACAATCGAA TCCTCAATGG 
CGGAAACTGT TGTGGTTGCA TAATGGTATT CATCTGGAGC ACAATACATC CGCCCGCGTC 
ATTCGCTCGA ATCAGAGCCT GGTACTCCAG AAGATTACGA AACACTACGC CGGGAACTAT 
GCGTGCAGTG CTATAAACGA CGAGGGCGAG ACGGTCAGCA ATCAGTTGCC ACTGCGGGTG 
AAATACACGC CCATGTGCAA GCACGCCGAC CGCGTAATAT TAATTGGCGC CTCTAAGGAC 
GAGACTGTTG AGGTTGTGTG CGAAATTCAA GCCGATCCGC CGCCGCGGAC TTTTCGCTGG 
AAATTCAACA ACTCGGGCGA AACTCTGGAC GTGGGCAGTG AACGATTCAG CGTGAACGGC 
AGCCGCAGCA TTTTAAAGTA CACACCAGTC ACAGATCAGG ATTATGGCAC ACTGTCATGC 
TGGGCCTCCA ACGAGGTGGG CACCCAGCAG CACCCCTGTC TCTTCCAAGT CGTGCTAGCC 
GCACTGCCGA ATGCCGTCAG CAATTGTACC GTCTTCAACA GAACTGAACT GAGTGTTGAC 
ATACAGTGCA TACCGGGCTA TGACGGCGGC CTGCCGCAAA TATTTGTACT CGAAATGTTT 
TCAACACGCA CCGGCATCAC CAGATTCAAT TTGAGCAACG CCGAGGAGGC ACTATTTTCG 
CTGGAGAACC TGGACACTCT GACCTCGATG ATGGTCCAGG AGAATAATTC GCTGCGGCTG 
CGCATCTACT CGTATAACCA GAAGGGCCGC AGTGCCGCTT ATCTGCTAAC CGATTTCATA 
ATTGGCTCAA CAGCTTACAA GACAGACGAC GATGTAACAC TGACATTGTC GCCGGTGATT 
GTCGGCAGTG TCCTGACCAT CATAATTATT GGTGTGGCCA TCGCGATACG AATATTCGTG 
GTGACATTTG TGCACAATTT GCGGCGGAAT CGTCATCATG GCAGCAAGGC CACCGCCGCC 
GGAGTAACCG CCCAGCCCAG CGATGTCTTC AAGGGCGGCA ACTTGGAGAA GCCGCGATGG 
CAGCACACGG ACATAAAAAC GAAATCATCA GAGGATTTGG TTGAGGACGA ACGTGATCCG 
GATGTAATAC CATCGCAATA CGTCGGTGGG AGCAGCGCCG GCAGCAGTGG CAGCAATGCC 
TCCAACGTGG GCAGCACCAC CGCTGGCACC GCCACCGGGA GCAGCACCTC CACGGCGGTG 
GTGGCCAACG CCAATGCGGC CGCGTCAGCG ATGGCTGGAC CGGGAGGAGC CACATCGTCA 
GCATCTGCCA CAGATGGACA TCAGTTTGCA TCTGGCACAG CGGCGGCAGG AGGCGGGGCC 
TCCAAGTGGT CACCCAATGG CAATGTGACG ACGACGATGC TGACCGGCGA TGCGGTGAAT 
GCAACTGTCA CCTACAATCA AATCAACGCG CAACGGGCGC AGCTGTTGGC GGCGGTGGCT 
GCAGTGGGCG GTGGATGCAG CAGCACCGTA ATCTCGCCCA GCAGCAGCAT ACTGGGCGGT 
CTTGGCAAGC CGCTGGGCAT GGGAATGGGC ATGGGCCTGG GCATGGGAGT GGGTGGCACG 
GTCATCGGGT CGCCCACGTC GCTATCGTCG ACGGCCACTT TGGCAGCTCA CCTGCAGCCG 
GCGCACAGTA GCGGAGTGGG CACATTGCCA CCGGGACTCG GAAGCGGCGG TGGCTACATA 
CTGAACGCTT ATGCCAGCGG GCGCCTGCAT CATCCACCTG GGGGTGGGGT GCCAGGCGGA 
GCGACTCACA CCGCCACACT GAACAGGCAT CACCACCAGC AGCAGCAGCT CCACCACCAC 
CCGCACCACC AGCAGCCACA CCACTCCGGC TCAAGTCTTT TGATGGGAAG TGCTGGAAGC 
GTGATGGGCG TTGTGGGATC CACCACGACA GCCACAACCT CGTGTAACTC CTCGCAGGAT 
CTGGACGATA ACATCAATAT AAATGCGATT AAGGACTGCC TCATGACGCA GCGAGTGCCC 
GAGAGTTGTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285946.1
CDS	1072..4266
Translation	MVLLLAMALLMPVTLARLHSTSGVGMSGGGSSMGPGGGGSGSGGISSKDSHVKTKDIDAVEGKSVSLPCPITEPLDNVYMVLWFRDNAGIPLYSFDVRDKESREQPRHWSAPEVFGSRAKFHFDSQPATLEIKRHDQGIYRCRVDFRTSQTQSFRFNLSVIILPEQPIIMDRWGRQLNGTQLGPKQEGDDIVITCRVVGGRPQPQVRWLVNGLLVDNQNEHNSGDVIENRLLWPSVQRNDLNSVFTCQALNTQLDKPKEKSFILDMHLKPLVVKILEPPSSMIADRRYEVSCESSGSRPNAIITWYKGKRQLRRTKDDISKNSTRSELSFVPTTDDDGKSITCRAENPNVNGLYLETMWKLNVVYPPLVTLRLGSTLTPDDIKEGDDVYFECHVQSNPQWRKLLWLHNGIHLEHNTSARVIRSNQSLVLQKITKHYAGNYACSAINDEGETVSNQLPLRVKYTPMCKHADRVILIGASKDETVEVVCEIQADPPPRTFRWKFNNSGETLDVGSERFSVNGSRSILKYTPVTDQDYGTLSCWASNEVGTQQHPCLFQVVLAALPNAVSNCTVFNRTELSVDIQCIPGYDGGLPQIFVLEMFSTRTGITRFNLSNAEEALFSLENLDTLTSMMVQENNSLRLRIYSYNQKGRSAAYLLTDFIIGSTAYKTDDDVTLTLSPVIVGSVLTIIIIGVAIAIRIFVVTFVHNLRRNRHHGSKATAAGVTAQPSDVFKGGNLEKPRWQHTDIKTKSSEDLVEDERDPDVIPSQYVGGSSAGSSGSNASNVGSTTAGTATGSSTSTAVVANANAAASAMAGPGGATSSASATDGHQFASGTAAAGGGASKWSPNGNVTTTMLTGDAVNATVTYNQINAQRAQLLAAVAAVGGGCSSTVISPSSSILGGLGKPLGMGMGMGLGMGVGGTVIGSPTSLSSTATLAAHLQPAHSSGVGTLPPGLGSGGGYILNAYASGRLHHPPGGGVPGGATHTATLNRHHHQQQQLHHHPHHQQPHHSGSSLLMGSAGSVMGVVGSTTTATTSCNSSQDLDDNININAIKDCLMTQRVPESCV

Target ORF information:

RefSeq Version	NM_001299017.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sidestep II (side-II), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299017.1

ORF Insert Sequence:

ATGGTCCTGC TTCTGGCCAT GGCACTGCTA ATGCCGGTCA CATTAGCGCG CCTGCATTCC 
ACTTCCGGCG TAGGAATGTC CGGCGGAGGC AGCAGCATGG GTCCTGGCGG AGGAGGATCC 
GGCAGCGGAG GCATATCCTC GAAGGATTCA CACGTGAAAA CGAAGGACAT TGATGCCGTC 
GAGGGAAAAT CGGTGTCGCT GCCCTGTCCC ATCACAGAGC CACTGGATAA TGTGTATATG 
GTGCTCTGGT TCCGCGATAA TGCCGGCATA CCGCTCTATA GCTTCGATGT GCGCGATAAG 
GAGAGTCGGG AGCAGCCACG ACATTGGTCA GCCCCGGAGG TTTTTGGCTC ACGTGCCAAA 
TTCCATTTTG ACTCGCAGCC AGCAACCTTG GAAATTAAGC GACATGATCA GGGTATTTAC 
CGTTGTCGTG TGGATTTTCG CACCAGTCAA ACGCAAAGTT TCCGTTTCAA TTTGTCAGTA 
ATAATACTTC CGGAGCAGCC AATCATCATG GACCGTTGGG GTCGTCAGCT GAATGGTACT 
CAATTGGGGC CCAAGCAGGA GGGCGATGAT ATCGTTATCA CTTGTCGTGT GGTGGGCGGT 
CGGCCGCAAC CGCAAGTCCG TTGGCTCGTA AATGGACTGC TTGTCGACAA TCAAAACGAG 
CACAATTCCG GCGATGTTAT CGAGAACCGT CTGTTGTGGC CATCGGTGCA GCGCAACGAT 
TTGAATTCCG TATTCACATG TCAGGCACTG AATACGCAAT TAGATAAACC CAAGGAGAAG 
AGCTTCATAC TGGATATGCA CTTGAAGCCT TTGGTTGTGA AAATCCTGGA GCCGCCCAGC 
TCGATGATTG CCGATCGCCG CTACGAGGTG TCCTGCGAAT CCTCCGGCTC TCGGCCAAAT 
GCCATCATCA CCTGGTACAA GGGAAAACGG CAATTACGAC GCACAAAGGA CGACATATCA 
AAGAACTCGA CACGCTCCGA GCTGAGCTTT GTGCCGACCA CAGACGACGA TGGCAAATCG 
ATAACATGCC GTGCGGAAAA TCCAAATGTG AATGGCCTGT ATTTGGAGAC CATGTGGAAA 
TTGAATGTCG TTTATCCTCC CTTGGTGACG TTACGCTTGG GCTCAACTTT GACACCGGAT 
GATATCAAGG AAGGCGACGA TGTTTACTTC GAGTGTCACG TACAATCGAA TCCTCAATGG 
CGGAAACTGT TGTGGTTGCA TAATGGTATT CATCTGGAGC ACAATACATC CGCCCGCGTC 
ATTCGCTCGA ATCAGAGCCT GGTACTCCAG AAGATTACGA AACACTACGC CGGGAACTAT 
GCGTGCAGTG CTATAAACGA CGAGGGCGAG ACGGTCAGCA ATCAGTTGCC ACTGCGGGTG 
AAATACACGC CCATGTGCAA GCACGCCGAC CGCGTAATAT TAATTGGCGC CTCTAAGGAC 
GAGACTGTTG AGGTTGTGTG CGAAATTCAA GCCGATCCGC CGCCGCGGAC TTTTCGCTGG 
AAATTCAACA ACTCGGGCGA AACTCTGGAC GTGGGCAGTG AACGATTCAG CGTGAACGGC 
AGCCGCAGCA TTTTAAAGTA CACACCAGTC ACAGATCAGG ATTATGGCAC ACTGTCATGC 
TGGGCCTCCA ACGAGGTGGG CACCCAGCAG CACCCCTGTC TCTTCCAAGT CGTGCTAGCC 
GCACTGCCGA ATGCCGTCAG CAATTGTACC GTCTTCAACA GAACTGAACT GAGTGTTGAC 
ATACAGTGCA TACCGGGCTA TGACGGCGGC CTGCCGCAAA TATTTGTACT CGAAATGTTT 
TCAACACGCA CCGGCATCAC CAGATTCAAT TTGAGCAACG CCGAGGAGGC ACTATTTTCG 
CTGGAGAACC TGGACACTCT GACCTCGATG ATGGTCCAGG AGAATAATTC GCTGCGGCTG 
CGCATCTACT CGTATAACCA GAAGGGCCGC AGTGCCGCTT ATCTGCTAAC CGATTTCATA 
ATTGGCTCAA CAGCTTACAA GACAGACGAC GATGTAACAC TGACATTGTC GCCGGTGATT 
GTCGGCAGTG TCCTGACCAT CATAATTATT GGTGTGGCCA TCGCGATACG AATATTCGTG 
GTGACATTTG TGCACAATTT GCGGCGGAAT CGTCATCATG GCAGCAAGGC CACCGCCGCC 
GGAGTAACCG CCCAGCCCAG CGATGTCTTC AAGGGCGGCA ACTTGGAGAA GCCGCGATGG 
CAGCACACGG ACATAAAAAC GAAATCATCA GAGGATTTGG TTGAGGACGA ACGTGATCCG 
GATGTAATAC CATCGCAATA CGTCGGTGGG AGCAGCGCCG GCAGCAGTGG CAGCAATGCC 
TCCAACGTGG GCAGCACCAC CGCTGGCACC GCCACCGGGA GCAGCACCTC CACGGCGGTG 
GTGGCCAACG CCAATGCGGC CGCGTCAGCG ATGGCTGGAC CGGGAGGAGC CACATCGTCA 
GCATCTGCCA CAGATGGACA TCAGTTTGCA TCTGGCACAG CGGCGGCAGG AGGCGGGGCC 
TCCAAGTGGT CACCCAATGG CAATGTGACG ACGACGATGC TGACCGGCGA TGCGGTGAAT 
GCAACTGTCA CCTACAATCA AATCAACGCG CAACGGGCGC AGCTGTTGGC GGCGGTGGCT 
GCAGTGGGCG GTGGATGCAG CAGCACCGTA ATCTCGCCCA GCAGCAGCAT ACTGGGCGGT 
CTTGGCAAGC CGCTGGGCAT GGGAATGGGC ATGGGCCTGG GCATGGGAGT GGGTGGCACG 
GTCATCGGGT CGCCCACGTC GCTATCGTCG ACGGCCACTT TGGCAGCTCA CCTGCAGCCG 
GCGCACAGTA GCGGAGTGGG CACATTGCCA CCGGGACTCG GAAGCGGCGG TGGCTACATA 
CTGAACGCTT ATGCCAGCGG GCGCCTGCAT CATCCACCTG GGGGTGGGGT GCCAGGCGGA 
GCGACTCACA CCGCCACACT GAACAGGCAT CACCACCAGC AGCAGCAGCT CCACCACCAC 
CCGCACCACC AGCAGCCACA CCACTCCGGC TCAAGTCTTT TGATGGGAAG TGCTGGAAGC 
GTGATGGGCG TTGTGGGATC CACCACGACA GCCACAACCT CGTGTAACTC CTCGCAGGAT 
CTGGACGATA ACATCAATAT AAATGCGATT AAGGACTGCC TCATGACGCA GCGAGTGCCC 
GAGAGTTGTG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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side-II cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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