Cwc25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cwc25
Entrez Gene ID	33460
Full Name	CG2843 gene product from transcript CG2843-RA
Synonyms	CG2843,CWC25,Dmel\CG2843
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23A3-23A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_134861.2	NP_608705.1	Cwc25

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cwc25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cwc25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13532	NM_134861.2 Latest version!	Drosophila melanogaster Cwc25 (Cwc25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cwc25 ( NM_134861.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608705.1 Drosophila melanogaster Cwc25 (Cwc25), mRNA.

Price & Availability↑

CloneID	OFa13532
Clone ID Related Accession (Same CDS sequence)	NM_134861.2
Accession Version	NM_134861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cwc25
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_134861.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTGGCG GAGATCTAAA TCTGAAGAAA TCATGGCATC CGCACACGAT GAAAAACCAG 
GAGCGCGTTT GGAAGGCGGA GGAGCAAGCG AAGATGGAGG AGCGCAAGCT GCAGGATCTG 
CGCAAGGAGA TCAACGAGGA GCGGGACAGA GAGGAACTAA GGCGATTGGG CGAAAGTTCC 
GGAGTGCTGA GCAATAATGG CGGAGCCGCC GGCGAGGCCA AGCTGGAGTG GATGTACAAA 
AACAGCACAG AGCTGATCAA CCGCGAGGAG TACCTCCTGG GCCGTAAGAT CGACAAGTCC 
TTTGAGACGC TGCAAGCGGA GGAAAGCCGC CAGGAGCAGA ACACAGTGGG TCTCAAGCAA 
ACGATCAACC ATGTGGAGCA CGACTGTGTG CCCTTCTCCA TCCGGACCTA CCGCAATGTG 
CAGTCCAACG AACAGGTGGA CATACAGCGC AAGACCCTCG AGGATCCCCT GATGCTCATC 
AAGCAGCGCG AAATGGAATC CCGTCGAAAG CTGCTTGAGA ACCCCGTCAA GCTGAAGGAA 
ATCCATCGCA TTCTCAAGAC TGAACAGGAA CAAAAGGCCG CAGAGGGAAA GAAGAAGAAC 
AAGAAGAACA AAAAGTCTAA GAAATCCAAA AAGAAGAAGA AGAACCGACG ATCCAGCAAT 
GAGGAAAGTA GTGACAGCGA GAGCAATGAT AGCGACGATG ACTTGGACAG AAAATTAGCC 
CGCCAAGTGA GCAAACTCAA GGGAGACCAA GGTGACCTTA AACTGGACAA GCTGCTGGAC 
GCCAAGTACC GCACCATTTC AAATCAATTA GACATGGCCA CCAAGAAGAA AAAGAGCAAG 
AAATCCAAGA AGAAGAAAAG CAGCGATAGC AGTGACGAAA GTAGTGACGA AGAGGAAGAG 
CCAAGGAGAA AGCACAAAAG GAGCCACAGC TCAGAGGACA GAAATCAGCG AGAAAGACGC 
AGGAGTAGAA GCCCCCGGGA TAGAAAAAAG CGACAGAGTA GAAGTCCGGA ACAAAGGCAA 
AGGCAAAGGA GTCCAGAAGA GAGAAGGGAA AGGCGCAGGA GCAGGAGCAG AAGTCCCTTA 
GCGAGAAGAC AGAGAAGAAC CAGGAGCCCA GAGGACAGAA AAGCCAGACG TCAAAGCAAG 
GACACTTCAA AGCAGCGCGA GAAACGCAGG AGTAGGACAC CTGAAGAGCG TTCCAGATCA 
AAACGCAGCA GGAGCCGAAG TTCCAAAGGA GATGACAAGC GAAGGATTGC CGAAAGACCG 
CCTCCCAGCC GTCCCACCGG CAAGCCAAAG TTAAGCGAAG CCGACCGAGA AGCTCGCCTG 
CGCGAAATGA TGGACAACGC CACTTGGAGA GAAGCGGATC GCAGCCAGGT GGTTCGCAAG 
CACCGAGAAG CATACGCGCG CGAGGAGGCC CAAAATCGTG AACGAGACTT CGACAAGGAG 
TTCATTAACA AGGAGGTGAA GAAGGCCATT GCCAACCATA ACTCAATTGG CGATCGCATC 
CGGGCCAATC TCAACAACAT ACAGCGCACC GCCTCCTCAA TGGACAGCAA CTTTGCCCGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608705.1
CDS	102..1667
Translation	MGGGDLNLKKSWHPHTMKNQERVWKAEEQAKMEERKLQDLRKEINEERDREELRRLGESSGVLSNNGGAAGEAKLEWMYKNSTELINREEYLLGRKIDKSFETLQAEESRQEQNTVGLKQTINHVEHDCVPFSIRTYRNVQSNEQVDIQRKTLEDPLMLIKQREMESRRKLLENPVKLKEIHRILKTEQEQKAAEGKKKNKKNKKSKKSKKKKKNRRSSNEESSDSESNDSDDDLDRKLARQVSKLKGDQGDLKLDKLLDAKYRTISNQLDMATKKKKSKKSKKKKSSDSSDESSDEEEEPRRKHKRSHSSEDRNQRERRRSRSPRDRKKRQSRSPEQRQRQRSPEERRERRRSRSRSPLARRQRRTRSPEDRKARRQSKDTSKQREKRRSRTPEERSRSKRSRSRSSKGDDKRRIAERPPPSRPTGKPKLSEADREARLREMMDNATWREADRSQVVRKHREAYAREEAQNRERDFDKEFINKEVKKAIANHNSIGDRIRANLNNIQRTASSMDSNFARK

Target ORF information:

RefSeq Version	NM_134861.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cwc25 (Cwc25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134861.2

ORF Insert Sequence:

ATGGGTGGCG GAGATCTAAA TCTGAAGAAA TCATGGCATC CGCACACGAT GAAAAACCAG 
GAGCGCGTTT GGAAGGCGGA GGAGCAAGCG AAGATGGAGG AGCGCAAGCT GCAGGATCTG 
CGCAAGGAGA TCAACGAGGA GCGGGACAGA GAGGAACTAA GGCGATTGGG CGAAAGTTCC 
GGAGTGCTGA GCAATAATGG CGGAGCCGCC GGCGAGGCCA AGCTGGAGTG GATGTACAAA 
AACAGCACAG AGCTGATCAA CCGCGAGGAG TACCTCCTGG GCCGTAAGAT CGACAAGTCC 
TTTGAGACGC TGCAAGCGGA GGAAAGCCGC CAGGAGCAGA ACACAGTGGG TCTCAAGCAA 
ACGATCAACC ATGTGGAGCA CGACTGTGTG CCCTTCTCCA TCCGGACCTA CCGCAATGTG 
CAGTCCAACG AACAGGTGGA CATACAGCGC AAGACCCTCG AGGATCCCCT GATGCTCATC 
AAGCAGCGCG AAATGGAATC CCGTCGAAAG CTGCTTGAGA ACCCCGTCAA GCTGAAGGAA 
ATCCATCGCA TTCTCAAGAC TGAACAGGAA CAAAAGGCCG CAGAGGGAAA GAAGAAGAAC 
AAGAAGAACA AAAAGTCTAA GAAATCCAAA AAGAAGAAGA AGAACCGACG ATCCAGCAAT 
GAGGAAAGTA GTGACAGCGA GAGCAATGAT AGCGACGATG ACTTGGACAG AAAATTAGCC 
CGCCAAGTGA GCAAACTCAA GGGAGACCAA GGTGACCTTA AACTGGACAA GCTGCTGGAC 
GCCAAGTACC GCACCATTTC AAATCAATTA GACATGGCCA CCAAGAAGAA AAAGAGCAAG 
AAATCCAAGA AGAAGAAAAG CAGCGATAGC AGTGACGAAA GTAGTGACGA AGAGGAAGAG 
CCAAGGAGAA AGCACAAAAG GAGCCACAGC TCAGAGGACA GAAATCAGCG AGAAAGACGC 
AGGAGTAGAA GCCCCCGGGA TAGAAAAAAG CGACAGAGTA GAAGTCCGGA ACAAAGGCAA 
AGGCAAAGGA GTCCAGAAGA GAGAAGGGAA AGGCGCAGGA GCAGGAGCAG AAGTCCCTTA 
GCGAGAAGAC AGAGAAGAAC CAGGAGCCCA GAGGACAGAA AAGCCAGACG TCAAAGCAAG 
GACACTTCAA AGCAGCGCGA GAAACGCAGG AGTAGGACAC CTGAAGAGCG TTCCAGATCA 
AAACGCAGCA GGAGCCGAAG TTCCAAAGGA GATGACAAGC GAAGGATTGC CGAAAGACCG 
CCTCCCAGCC GTCCCACCGG CAAGCCAAAG TTAAGCGAAG CCGACCGAGA AGCTCGCCTG 
CGCGAAATGA TGGACAACGC CACTTGGAGA GAAGCGGATC GCAGCCAGGT GGTTCGCAAG 
CACCGAGAAG CATACGCGCG CGAGGAGGCC CAAAATCGTG AACGAGACTT CGACAAGGAG 
TTCATTAACA AGGAGGTGAA GAAGGCCATT GCCAACCATA ACTCAATTGG CGATCGCATC 
CGGGCCAATC TCAACAACAT ACAGCGCACC GCCTCCTCAA TGGACAGCAA CTTTGCCCGC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cwc25 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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