wry cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wry
Entrez Gene ID	33361
Full Name	weary
Synonyms	CG12704,CG15621,CG31665,CT35769,Dmel\CG31665
General protein information	Preferred Names weary Names CG31665 gene product from transcript CG31665-RD CG31665-PB CG31665-PC CG31665-PD fibropellin wry-PB wry-PC wry-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 22B3-22B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_205889.4	NP_995611.2	weary, isoform B
NM_001258913.2	NP_001245842.1	weary, isoform C
NM_164443.4	NP_722737.3	weary, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010049	XP_319202.4
Drosophila melanogaster (fruit fly)	wry	NP_001245842.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identify CG31665/Wry as a previously unknown Notch ligand in Drosophila that when deleted causes cardiomyopathy.
Title: Gene deletion screen for cardiomyopathy in adult Drosophila identifies a new notch ligand.

The following wry gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wry cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13465	NM_001258913.2 Latest version!	Drosophila melanogaster weary (wry), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16090	NM_164443.4 Latest version!	Drosophila melanogaster weary (wry), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa16091	NM_205889.4 Latest version!	Drosophila melanogaster weary (wry), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wry ( NM_001258913.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245842.1 Drosophila melanogaster weary (wry), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13465
Clone ID Related Accession (Same CDS sequence)	NM_001258913.2
Accession Version	NM_001258913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	weary, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001258913.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCGCCGTTCA CGCCACACAG GTGTATCAGG TAACTCGTTC CGGCCACGAA 
CACTGCGATG TGACCGAGGG CGTCCTGCTG GATATCACGC CGCTGGTGGT CGACGGCAGG 
AAGCTGGTTA CTCTGTACGA TAAGGACCTC ACCGAGGGAG TTAACCTGCT AATTGTGGTG 
TCCGAGTTGT GGGGCGCGCA GTGTGTACGC CTGAAGGTGA CCACCAAAAC CGACAATTGC 
GGCGAGAACG CCGATTGTTC CGGCAAGGGA GTGTGCTACT CCAACTCCGG AATGGAGGAG 
TACGAGTGCC AGTGCTGCAG CGGATTCGCA GGACCACACT GCGAGGAGAT AGACGCCTGC 
AATCCGAGTC CTTGCACCAA CAACGGCATC TGCGTGGACC TCTCGCAGGG CCACGAGGGC 
AACTCGTACC AGTGCCTGTG TCCGTACGGA TACGCGGGCA AGAACTGTCA ATACGAGTCG 
GATCCCTGCA ATCCCGCCGA GTGCATGAAT GGCGGCAGCT GTGTGGGCAA CTCGACGCAC 
TTTCGCTGCG ACTGTGCTCC CGGGTTCACA GGACCTCTTT GCCAGCACAG CCTGAATGAG 
TGCGAGTCCT CGCCGTGTGT TCACGGCATC TGCGTCGACC AGGAGGACGG ATTCCGCTGC 
TTCTGCCAGC CAGGATTCGC CGGCGAGCTG TGCAACTTCG AGTATAATGA ATGCGAATCG 
AATCCGTGTC AGAATGGCGG CGAGTGCATC GATCACATTG GCAGCTACGA GTGTCGCTGC 
ACGAAGGGAT TCAGCGGCAA CCGCTGCCAA ATTAAGGTTG ATTTCTGCGC CAACAAGCCC 
TGCCCCGAAG GACATCGCTG TATTGATCAT GGAAATGATT TCAGCTGCGA ATGCCCAGGA 
GGTCGCAACG GACCGGATTG TAATCAAATG CCACGAACGC AATGCAACGT GAATCCGTGC 
ACCCATGGCG GCACTTGTTG GTCCAGCGGC GATAGCTTCT ACTGCGCCTG TCGTCCCGGC 
TACACAGGAA CCATGTGCGA AGATGAGTTC GTGGTGGAGA CGGTCGTTAG TTCCAGCGAA 
TTTATTGTGG ACGATGCGAA CGCCAGAAAT TTTAATGACA AAACTTTCGG TTCATCGGTT 
GTGCTTAAGA GTCCCATTGA ATTGCATAAT GCATATTTTG CGGCCGGAGT CCTGGCAGCC 
GCAATTTTCA TCGTTGCCGT TGTGGTCACC ATTTGTCACT GCAAGGTCAA TCAGACTTAT 
CGAAAATTCT CCACACGTTC CACCTCGTTT ATACCCATAC TGGGCTTCAG TCGCCGCCCG 
AAAGTGCAGG GCAAACTGAA CAAACACTGG CTAAGTGGCA AGGGGGCCAC CGCGACCAGC 
AGCACCAATA TTGTGGCGCC CCCGGGTGGA CCGAGGGCCG GAGGAGCAGC AGGGCCGCAG 
CAGGTAACCC GACATCTGCC TGGACAGCCT CAGACGCAGA CCACACTGGG TGGCCAGCTG 
CAGGAGCGAC CTTTTCTGCG GCACCTGGCA ATGAATCTGG AGAACGACAT GTACTACACG 
GTGGACTTTA GCGAGAACTC ACAGCACTCG CCGTTGATAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245842.1
CDS	216..3680
Translation	MWKIYVLTLVIAVVAATTSETITNDLTAKSNRTLLKTSTEESREISALGGSTSTATPTTTATTSTTTTRATTVAEDEMEETSTPTPTTVRLMTTTQKMVQANLGEIGSIKDALHRIKLSEKPVDGATTTTEQPEAMDQDEQDEQDVTPTISPLQIQMQAAASYQVAETLADLNEEERARYDAMLAQQPTASKLNIVDLYPLKIEDIQPIIRNDNDELIKQRTVFTQHENAEEYKQNLMPNEVEMMVDKEVIEMKDKIQINQEKFGSSTTVKPTTSRPHQGTTADFTGKLLADQDDLITEIESKFQLHETTTLRPRVTTVQHPGNTFIDRRVKKSFEFPMQHRASDVMAMPHIDFANTKFQTDADAPNPPASHPEFSTTKFYNSKELYNEMLLHNKRKLMKAAKAEGSPIKSAEDTTRATSSNVTPEGKTFGPTTTTAAGSTATTTAESTTTAATTTRLPATTTGKYQKELKRAKLLLKALTPPKSVDKSTASPSSTTSTSTSTTTTMATLLATARTRPSAANPTKTASKPIARPRILSRLQEKINSLECEIPNVPQDSHLWRGNETHELLLPIVTTEHCKPDEHCVPNVLTWRGEAEIQSGDILIVEINDAMLNPSHEPNNTSSAVHATQVYQVTRSGHEHCDVTEGVLLDITPLVVDGRKLVTLYDKDLTEGVNLLIVVSELWGAQCVRLKVTTKTDNCGENADCSGKGVCYSNSGMEEYECQCCSGFAGPHCEEIDACNPSPCTNNGICVDLSQGHEGNSYQCLCPYGYAGKNCQYESDPCNPAECMNGGSCVGNSTHFRCDCAPGFTGPLCQHSLNECESSPCVHGICVDQEDGFRCFCQPGFAGELCNFEYNECESNPCQNGGECIDHIGSYECRCTKGFSGNRCQIKVDFCANKPCPEGHRCIDHGNDFSCECPGGRNGPDCNQMPRTQCNVNPCTHGGTCWSSGDSFYCACRPGYTGTMCEDEFVVETVVSSSEFIVDDANARNFNDKTFGSSVVLKSPIELHNAYFAAGVLAAAIFIVAVVVTICHCKVNQTYRKFSTRSTSFIPILGFSRRPKVQGKLNKHWLSGKGATATSSTNIVAPPGGPRAGGAAGPQQVTRHLPGQPQTQTTLGGQLQERPFLRHLAMNLENDMYYTVDFSENSQHSPLIQ

Target ORF information:

RefSeq Version	NM_001258913.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster weary (wry), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258913.2

ORF Insert Sequence:

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCGCCGTTCA CGCCACACAG GTGTATCAGG TAACTCGTTC CGGCCACGAA 
CACTGCGATG TGACCGAGGG CGTCCTGCTG GATATCACGC CGCTGGTGGT CGACGGCAGG 
AAGCTGGTTA CTCTGTACGA TAAGGACCTC ACCGAGGGAG TTAACCTGCT AATTGTGGTG 
TCCGAGTTGT GGGGCGCGCA GTGTGTACGC CTGAAGGTGA CCACCAAAAC CGACAATTGC 
GGCGAGAACG CCGATTGTTC CGGCAAGGGA GTGTGCTACT CCAACTCCGG AATGGAGGAG 
TACGAGTGCC AGTGCTGCAG CGGATTCGCA GGACCACACT GCGAGGAGAT AGACGCCTGC 
AATCCGAGTC CTTGCACCAA CAACGGCATC TGCGTGGACC TCTCGCAGGG CCACGAGGGC 
AACTCGTACC AGTGCCTGTG TCCGTACGGA TACGCGGGCA AGAACTGTCA ATACGAGTCG 
GATCCCTGCA ATCCCGCCGA GTGCATGAAT GGCGGCAGCT GTGTGGGCAA CTCGACGCAC 
TTTCGCTGCG ACTGTGCTCC CGGGTTCACA GGACCTCTTT GCCAGCACAG CCTGAATGAG 
TGCGAGTCCT CGCCGTGTGT TCACGGCATC TGCGTCGACC AGGAGGACGG ATTCCGCTGC 
TTCTGCCAGC CAGGATTCGC CGGCGAGCTG TGCAACTTCG AGTATAATGA ATGCGAATCG 
AATCCGTGTC AGAATGGCGG CGAGTGCATC GATCACATTG GCAGCTACGA GTGTCGCTGC 
ACGAAGGGAT TCAGCGGCAA CCGCTGCCAA ATTAAGGTTG ATTTCTGCGC CAACAAGCCC 
TGCCCCGAAG GACATCGCTG TATTGATCAT GGAAATGATT TCAGCTGCGA ATGCCCAGGA 
GGTCGCAACG GACCGGATTG TAATCAAATG CCACGAACGC AATGCAACGT GAATCCGTGC 
ACCCATGGCG GCACTTGTTG GTCCAGCGGC GATAGCTTCT ACTGCGCCTG TCGTCCCGGC 
TACACAGGAA CCATGTGCGA AGATGAGTTC GTGGTGGAGA CGGTCGTTAG TTCCAGCGAA 
TTTATTGTGG ACGATGCGAA CGCCAGAAAT TTTAATGACA AAACTTTCGG TTCATCGGTT 
GTGCTTAAGA GTCCCATTGA ATTGCATAAT GCATATTTTG CGGCCGGAGT CCTGGCAGCC 
GCAATTTTCA TCGTTGCCGT TGTGGTCACC ATTTGTCACT GCAAGGTCAA TCAGACTTAT 
CGAAAATTCT CCACACGTTC CACCTCGTTT ATACCCATAC TGGGCTTCAG TCGCCGCCCG 
AAAGTGCAGG GCAAACTGAA CAAACACTGG CTAAGTGGCA AGGGGGCCAC CGCGACCAGC 
AGCACCAATA TTGTGGCGCC CCCGGGTGGA CCGAGGGCCG GAGGAGCAGC AGGGCCGCAG 
CAGGTAACCC GACATCTGCC TGGACAGCCT CAGACGCAGA CCACACTGGG TGGCCAGCTG 
CAGGAGCGAC CTTTTCTGCG GCACCTGGCA ATGAATCTGG AGAACGACAT GTACTACACG 
GTGGACTTTA GCGAGAACTC ACAGCACTCG CCGTTGATAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wry ( NM_164443.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722737.3 Drosophila melanogaster weary (wry), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa16090
Clone ID Related Accession (Same CDS sequence)	NM_164443.4
Accession Version	NM_164443.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	weary, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164443.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCAGCGCCGT TCACGCCACA CAGGTGTATC AGGTAACTCG TTCCGGCCAC 
GAACACTGCG ATGTGACCGA GGGCGTCCTG CTGGATATCA CGCCGCTGGT GGTCGACGGC 
AGGAAGCTGG TTACTCTGTA CGATAAGGAC CTCACCGAGG GAGTTAACCT GCTAATTGTG 
GTGTCCGAGT TGTGGGGCGC GCAGTGTGTA CGCCTGAAGG TGACCACCAA AACCGACAAT 
TGCGGCGAGA ACGCCGATTG TTCCGGCAAG GGAGTGTGCT ACTCCAACTC CGGAATGGAG 
GAGTACGAGT GCCAGTGCTG CAGCGGATTC GCAGGACCAC ACTGCGAGGA GATAGACGCC 
TGCAATCCGA GTCCTTGCAC CAACAACGGC ATCTGCGTGG ACCTCTCGCA GGGCCACGAG 
GGCAACTCGT ACCAGTGCCT GTGTCCGTAC GGATACGCGG GCAAGAACTG TCAATACGAG 
TCGGATCCCT GCAATCCCGC CGAGTGCATG AATGGCGGCA GCTGTGTGGG CAACTCGACG 
CACTTTCGCT GCGACTGTGC TCCCGGGTTC ACAGGACCTC TTTGCCAGCA CAGCCTGAAT 
GAGTGCGAGT CCTCGCCGTG TGTTCACGGC ATCTGCGTCG ACCAGGAGGA CGGATTCCGC 
TGCTTCTGCC AGCCAGGATT CGCCGGCGAG CTGTGCAACT TCGAGTATAA TGAATGCGAA 
TCGAATCCGT GTCAGAATGG CGGCGAGTGC ATCGATCACA TTGGCAGCTA CGAGTGTCGC 
TGCACGAAGG GATTCAGCGG CAACCGCTGC CAAATTAAGG TTGATTTCTG CGCCAACAAG 
CCCTGCCCCG AAGGACATCG CTGTATTGAT CATGGAAATG ATTTCAGCTG CGAATGCCCA 
GGAGGTCGCA ACGGACCGGA TTGTAATCAA ATGCCACGAA CGCAATGCAA CGTGAATCCG 
TGCACCCATG GCGGCACTTG TTGGTCCAGC GGCGATAGCT TCTACTGCGC CTGTCGTCCC 
GGCTACACAG GAACCATGTG CGAAGATGAG TTCGTGGTGG AGACGGTCGT TAGTTCCAGC 
GAATTTATTG TGGACGATGC GAACGCCAGA AATTTTAATG ACAAAACTTT CGGTTCATCG 
GTTGTGCTTA AGAGTCCCAT TGAATTGCAT AATGCATATT TTGCGGCCGG AGTCCTGGCA 
GCCGCAATTT TCATCGTTGC CGTTGTGGTC ACCATTTGTC ACTGCAAGGT CAATCAGACT 
TATCGAAAAT TCTCCACACG TTCCACCTCG TTTATACCCA TACTGGGCTT CAGTCGCCGC 
CCGAAAGTGC AGGGCAAACT GAACAAACAC TGGCTAAGTG GCAAGGGGGC CACCGCGACC 
AGCAGCACCA ATATTGTGGC GCCCCCGGGT GGACCGAGGG CCGGAGGAGC AGCAGGGCCG 
CAGCAGGTAA CCCGACATCT GCCTGGACAG CCTCAGACGC AGACCACACT GGGTGGCCAG 
CTGCAGGAGC GACCTTTTCT GCGGCACCTG GCAATGAATC TGGAGAACGA CATGTACTAC 
ACGGTGGACT TTAGCGAGAA CTCACAGCAC TCGCCGTTGA TACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722737.3
CDS	450..3917
Translation	MWKIYVLTLVIAVVAATTSETITNDLTAKSNRTLLKTSTEESREISALGGSTSTATPTTTATTSTTTTRATTVAEDEMEETSTPTPTTVRLMTTTQKMVQANLGEIGSIKDALHRIKLSEKPVDGATTTTEQPEAMDQDEQDEQDVTPTISPLQIQMQAAASYQVAETLADLNEEERARYDAMLAQQPTASKLNIVDLYPLKIEDIQPIIRNDNDELIKQRTVFTQHENAEEYKQNLMPNEVEMMVDKEVIEMKDKIQINQEKFGSSTTVKPTTSRPHQGTTADFTGKLLADQDDLITEIESKFQLHETTTLRPRVTTVQHPGNTFIDRRVKKSFEFPMQHRASDVMAMPHIDFANTKFQTDADAPNPPASHPEFSTTKFYNSKELYNEMLLHNKRKLMKAAKAEGSPIKSAEDTTRATSSNVTPEGKTFGPTTTTAAGSTATTTAESTTTAATTTRLPATTTGKYQKELKRAKLLLKALTPPKSVDKSTASPSSTTSTSTSTTTTMATLLATARTRPSAANPTKTASKPIARPRILSRLQEKINSLECEIPNVPQDSHLWRGNETHELLLPIVTTEHCKPDEHCVPNVLTWRGEAEIQSGDILIVEINDAMLNPSHEPNNTSSSAVHATQVYQVTRSGHEHCDVTEGVLLDITPLVVDGRKLVTLYDKDLTEGVNLLIVVSELWGAQCVRLKVTTKTDNCGENADCSGKGVCYSNSGMEEYECQCCSGFAGPHCEEIDACNPSPCTNNGICVDLSQGHEGNSYQCLCPYGYAGKNCQYESDPCNPAECMNGGSCVGNSTHFRCDCAPGFTGPLCQHSLNECESSPCVHGICVDQEDGFRCFCQPGFAGELCNFEYNECESNPCQNGGECIDHIGSYECRCTKGFSGNRCQIKVDFCANKPCPEGHRCIDHGNDFSCECPGGRNGPDCNQMPRTQCNVNPCTHGGTCWSSGDSFYCACRPGYTGTMCEDEFVVETVVSSSEFIVDDANARNFNDKTFGSSVVLKSPIELHNAYFAAGVLAAAIFIVAVVVTICHCKVNQTYRKFSTRSTSFIPILGFSRRPKVQGKLNKHWLSGKGATATSSTNIVAPPGGPRAGGAAGPQQVTRHLPGQPQTQTTLGGQLQERPFLRHLAMNLENDMYYTVDFSENSQHSPLIQ

Target ORF information:

RefSeq Version	NM_164443.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster weary (wry), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164443.4

ORF Insert Sequence:

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCAGCGCCGT TCACGCCACA CAGGTGTATC AGGTAACTCG TTCCGGCCAC 
GAACACTGCG ATGTGACCGA GGGCGTCCTG CTGGATATCA CGCCGCTGGT GGTCGACGGC 
AGGAAGCTGG TTACTCTGTA CGATAAGGAC CTCACCGAGG GAGTTAACCT GCTAATTGTG 
GTGTCCGAGT TGTGGGGCGC GCAGTGTGTA CGCCTGAAGG TGACCACCAA AACCGACAAT 
TGCGGCGAGA ACGCCGATTG TTCCGGCAAG GGAGTGTGCT ACTCCAACTC CGGAATGGAG 
GAGTACGAGT GCCAGTGCTG CAGCGGATTC GCAGGACCAC ACTGCGAGGA GATAGACGCC 
TGCAATCCGA GTCCTTGCAC CAACAACGGC ATCTGCGTGG ACCTCTCGCA GGGCCACGAG 
GGCAACTCGT ACCAGTGCCT GTGTCCGTAC GGATACGCGG GCAAGAACTG TCAATACGAG 
TCGGATCCCT GCAATCCCGC CGAGTGCATG AATGGCGGCA GCTGTGTGGG CAACTCGACG 
CACTTTCGCT GCGACTGTGC TCCCGGGTTC ACAGGACCTC TTTGCCAGCA CAGCCTGAAT 
GAGTGCGAGT CCTCGCCGTG TGTTCACGGC ATCTGCGTCG ACCAGGAGGA CGGATTCCGC 
TGCTTCTGCC AGCCAGGATT CGCCGGCGAG CTGTGCAACT TCGAGTATAA TGAATGCGAA 
TCGAATCCGT GTCAGAATGG CGGCGAGTGC ATCGATCACA TTGGCAGCTA CGAGTGTCGC 
TGCACGAAGG GATTCAGCGG CAACCGCTGC CAAATTAAGG TTGATTTCTG CGCCAACAAG 
CCCTGCCCCG AAGGACATCG CTGTATTGAT CATGGAAATG ATTTCAGCTG CGAATGCCCA 
GGAGGTCGCA ACGGACCGGA TTGTAATCAA ATGCCACGAA CGCAATGCAA CGTGAATCCG 
TGCACCCATG GCGGCACTTG TTGGTCCAGC GGCGATAGCT TCTACTGCGC CTGTCGTCCC 
GGCTACACAG GAACCATGTG CGAAGATGAG TTCGTGGTGG AGACGGTCGT TAGTTCCAGC 
GAATTTATTG TGGACGATGC GAACGCCAGA AATTTTAATG ACAAAACTTT CGGTTCATCG 
GTTGTGCTTA AGAGTCCCAT TGAATTGCAT AATGCATATT TTGCGGCCGG AGTCCTGGCA 
GCCGCAATTT TCATCGTTGC CGTTGTGGTC ACCATTTGTC ACTGCAAGGT CAATCAGACT 
TATCGAAAAT TCTCCACACG TTCCACCTCG TTTATACCCA TACTGGGCTT CAGTCGCCGC 
CCGAAAGTGC AGGGCAAACT GAACAAACAC TGGCTAAGTG GCAAGGGGGC CACCGCGACC 
AGCAGCACCA ATATTGTGGC GCCCCCGGGT GGACCGAGGG CCGGAGGAGC AGCAGGGCCG 
CAGCAGGTAA CCCGACATCT GCCTGGACAG CCTCAGACGC AGACCACACT GGGTGGCCAG 
CTGCAGGAGC GACCTTTTCT GCGGCACCTG GCAATGAATC TGGAGAACGA CATGTACTAC 
ACGGTGGACT TTAGCGAGAA CTCACAGCAC TCGCCGTTGA TACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wry ( NM_205889.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995611.2 Drosophila melanogaster weary (wry), transcript variant B, mRNA.

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CloneID	OFa16091
Clone ID Related Accession (Same CDS sequence)	NM_205889.4
Accession Version	NM_205889.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	weary, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205889.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCGCCGTTCA CGCCACACAG GTGTATCAGG TAACTCGTTC CGGCCACGAA 
CACTGCGATG TGACCGAGGG CGTCCTGCTG GATATCACGC CGCTGGTGGT CGACGGCAGG 
AAGCTGGTTA CTCTGTACGA TAAGGACCTC ACCGAGGGAG TTAACCTGCT AATTGTGGTG 
TCCGAGTTGT GGGGCGCGCA GTGTGTACGC CTGAAGGTGA CCACCAAAAC CGACAATTGC 
GGCGAGAACG CCGATTGTTC CGGCAAGGGA GTGTGCTACT CCAACTCCGG AATGGAGGAG 
TACGAGTGCC AGTGCTGCAG CGGATTCGCA GGACCACACT GCGAGGAGAT AGACGCCTGC 
AATCCGAGTC CTTGCACCAA CAACGGCATC TGCGTGGACC TCTCGCAGGG CCACGAGGGC 
AACTCGTACC AGTGCCTGTG TCCGTACGGA TACGCGGGCA AGAACTGTCA ATACGAGTCG 
GATCCCTGCA ATCCCGCCGA GTGCATGAAT GGCGGCAGCT GTGTGGGCAA CTCGACGCAC 
TTTCGCTGCG ACTGTGCTCC CGGGTTCACA GGACCTCTTT GCCAGCACAG CCTGAATGAG 
TGCGAGTCCT CGCCGTGTGT TCACGGCATC TGCGTCGACC AGGAGGACGG ATTCCGCTGC 
TTCTGCCAGC CAGGATTCGC CGGCGAGCTG TGCAACTTCG AGTATAATGA ATGCGAATCG 
AATCCGTGTC AGAATGGCGG CGAGTGCATC GATCACATTG GCAGCTACGA GTGTCGCTGC 
ACGAAGGGAT TCAGCGGCAA CCGCTGCCAA ATTAAGGTTG ATTTCTGCGC CAACAAGCCC 
TGCCCCGAAG GACATCGCTG TATTGATCAT GGAAATGATT TCAGCTGCGA ATGCCCAGGA 
GGTCGCAACG GACCGGATTG TAATCAAATG CCACGAACGT ACTTCCAGCA ATGCAACGTG 
AATCCGTGCA CCCATGGCGG CACTTGTTGG TCCAGCGGCG ATAGCTTCTA CTGCGCCTGT 
CGTCCCGGCT ACACAGGAAC CATGTGCGAA GATGAGTTCG TGGTGGAGAC GGTCGTTAGT 
TCCAGCGAAT TTATTGTGGA CGATGCGAAC GCCAGAAATT TTAATGACAA AACTTTCGGT 
TCATCGGTTG TGCTTAAGAG TCCCATTGAA TTGCATAATG CATATTTTGC GGCCGGAGTC 
CTGGCAGCCG CAATTTTCAT CGTTGCCGTT GTGGTCACCA TTTGTCACTG CAAGGTCAAT 
CAGACTTATC GAAAATTCTC CACACGTTCC ACCTCGTTTA TACCCATACT GGGCTTCAGT 
CGCCGCCCGA AAGTGCAGGG CAAACTGAAC AAACACTGGC TAAGTGGCAA GGGGGCCACC 
GCGACCAGCA GCACCAATAT TGTGGCGCCC CCGGGTGGAC CGAGGGCCGG AGGAGCAGCA 
GGGCCGCAGC AGGTAACCCG ACATCTGCCT GGACAGCCTC AGACGCAGAC CACACTGGGT 
GGCCAGCTGC AGGAGCGACC TTTTCTGCGG CACCTGGCAA TGAATCTGGA GAACGACATG 
TACTACACGG TGGACTTTAG CGAGAACTCA CAGCACTCGC CGTTGATACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995611.2
CDS	450..3923
Translation	MWKIYVLTLVIAVVAATTSETITNDLTAKSNRTLLKTSTEESREISALGGSTSTATPTTTATTSTTTTRATTVAEDEMEETSTPTPTTVRLMTTTQKMVQANLGEIGSIKDALHRIKLSEKPVDGATTTTEQPEAMDQDEQDEQDVTPTISPLQIQMQAAASYQVAETLADLNEEERARYDAMLAQQPTASKLNIVDLYPLKIEDIQPIIRNDNDELIKQRTVFTQHENAEEYKQNLMPNEVEMMVDKEVIEMKDKIQINQEKFGSSTTVKPTTSRPHQGTTADFTGKLLADQDDLITEIESKFQLHETTTLRPRVTTVQHPGNTFIDRRVKKSFEFPMQHRASDVMAMPHIDFANTKFQTDADAPNPPASHPEFSTTKFYNSKELYNEMLLHNKRKLMKAAKAEGSPIKSAEDTTRATSSNVTPEGKTFGPTTTTAAGSTATTTAESTTTAATTTRLPATTTGKYQKELKRAKLLLKALTPPKSVDKSTASPSSTTSTSTSTTTTMATLLATARTRPSAANPTKTASKPIARPRILSRLQEKINSLECEIPNVPQDSHLWRGNETHELLLPIVTTEHCKPDEHCVPNVLTWRGEAEIQSGDILIVEINDAMLNPSHEPNNTSSAVHATQVYQVTRSGHEHCDVTEGVLLDITPLVVDGRKLVTLYDKDLTEGVNLLIVVSELWGAQCVRLKVTTKTDNCGENADCSGKGVCYSNSGMEEYECQCCSGFAGPHCEEIDACNPSPCTNNGICVDLSQGHEGNSYQCLCPYGYAGKNCQYESDPCNPAECMNGGSCVGNSTHFRCDCAPGFTGPLCQHSLNECESSPCVHGICVDQEDGFRCFCQPGFAGELCNFEYNECESNPCQNGGECIDHIGSYECRCTKGFSGNRCQIKVDFCANKPCPEGHRCIDHGNDFSCECPGGRNGPDCNQMPRTYFQQCNVNPCTHGGTCWSSGDSFYCACRPGYTGTMCEDEFVVETVVSSSEFIVDDANARNFNDKTFGSSVVLKSPIELHNAYFAAGVLAAAIFIVAVVVTICHCKVNQTYRKFSTRSTSFIPILGFSRRPKVQGKLNKHWLSGKGATATSSTNIVAPPGGPRAGGAAGPQQVTRHLPGQPQTQTTLGGQLQERPFLRHLAMNLENDMYYTVDFSENSQHSPLIQ

Target ORF information:

RefSeq Version	NM_205889.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster weary (wry), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205889.4

ORF Insert Sequence:

ATGTGGAAAA TCTACGTTTT AACATTGGTA ATTGCGGTGG TGGCAGCAAC AACTAGTGAA 
ACCATAACAA ATGACTTGAC TGCGAAAAGC AACAGAACGC TTTTGAAAAC TTCCACGGAG 
GAATCGAGGG AAATTTCAGC TCTGGGCGGG TCAACCTCAA CGGCCACGCC CACCACGACG 
GCCACAACTA GCACAACAAC GACCAGGGCA ACAACCGTGG CGGAGGATGA AATGGAGGAA 
ACTTCCACAC CCACACCAAC TACAGTCAGG CTAATGACAA CCACCCAAAA GATGGTTCAA 
GCCAATTTGG GTGAAATAGG CAGCATCAAG GATGCACTGC ATCGGATTAA ACTGAGCGAA 
AAGCCAGTGG ATGGAGCGAC AACCACCACT GAGCAACCAG AAGCCATGGA TCAGGATGAG 
CAGGATGAGC AGGATGTAAC CCCCACAATT TCCCCGCTGC AGATTCAGAT GCAGGCTGCT 
GCCTCATATC AAGTAGCCGA AACGTTGGCC GACTTGAACG AGGAGGAACG GGCTAGATAC 
GACGCCATGT TGGCCCAGCA GCCCACGGCC AGCAAACTGA ATATAGTGGA CCTATATCCA 
CTGAAAATCG AGGACATTCA GCCAATTATC CGGAACGATA ATGACGAGTT GATTAAGCAG 
AGAACCGTGT TCACCCAGCA TGAAAATGCC GAGGAATACA AGCAGAATCT GATGCCCAAC 
GAAGTGGAGA TGATGGTCGA CAAAGAGGTG ATTGAGATGA AGGACAAGAT TCAAATAAAT 
CAGGAAAAGT TCGGCAGCTC GACCACAGTG AAGCCCACAA CGAGCAGACC GCACCAGGGA 
ACCACAGCCG ATTTCACGGG CAAACTGCTG GCCGATCAGG ATGATTTGAT TACCGAAATC 
GAGAGCAAGT TCCAGCTGCA TGAAACGACA ACTTTGAGGC CCAGAGTTAC GACCGTGCAG 
CATCCGGGCA ATACCTTTAT TGATCGCCGG GTCAAGAAGA GCTTCGAGTT TCCCATGCAG 
CATCGGGCCA GCGATGTGAT GGCCATGCCG CACATTGACT TTGCCAACAC CAAGTTCCAA 
ACGGATGCCG ACGCGCCCAA TCCTCCAGCC TCGCATCCCG AATTCTCCAC CACAAAGTTT 
TACAACAGCA AGGAGCTGTA CAACGAAATG CTGCTCCACA ATAAGCGCAA GTTAATGAAG 
GCAGCCAAGG CTGAAGGCAG TCCGATAAAG TCAGCGGAGG ATACGACTCG GGCCACAAGC 
TCGAACGTGA CGCCAGAAGG GAAAACTTTT GGCCCAACCA CAACTACAGC GGCAGGAAGC 
ACCGCAACAA CAACTGCTGA GTCAACTACC ACGGCAGCAA CAACAACCAG ACTCCCGGCA 
ACAACAACTG GAAAATATCA GAAGGAGCTT AAGCGTGCCA AATTATTGCT CAAGGCCTTG 
ACACCGCCAA AGTCGGTTGA CAAGTCGACG GCATCCCCAT CATCGACCAC CAGCACTAGC 
ACCAGCACCA CCACAACGAT GGCCACTCTG CTGGCCACCG CCAGGACTCG TCCGTCCGCA 
GCTAATCCCA CTAAGACCGC TTCAAAGCCA ATCGCCAGGC CGCGCATCCT GAGTCGCCTG 
CAGGAGAAAA TCAATTCGCT CGAGTGCGAG ATTCCCAATG TGCCGCAGGA CTCGCATCTG 
TGGCGCGGCA ACGAGACTCA CGAGCTGCTG CTGCCCATTG TGACCACGGA ACACTGCAAA 
CCGGACGAGC ACTGCGTCCC GAATGTCCTG ACTTGGCGAG GTGAGGCAGA GATCCAGTCC 
GGCGACATTT TAATTGTGGA AATCAATGAT GCCATGCTGA ATCCTTCGCA CGAGCCGAAC 
AACACGAGCA GCGCCGTTCA CGCCACACAG GTGTATCAGG TAACTCGTTC CGGCCACGAA 
CACTGCGATG TGACCGAGGG CGTCCTGCTG GATATCACGC CGCTGGTGGT CGACGGCAGG 
AAGCTGGTTA CTCTGTACGA TAAGGACCTC ACCGAGGGAG TTAACCTGCT AATTGTGGTG 
TCCGAGTTGT GGGGCGCGCA GTGTGTACGC CTGAAGGTGA CCACCAAAAC CGACAATTGC 
GGCGAGAACG CCGATTGTTC CGGCAAGGGA GTGTGCTACT CCAACTCCGG AATGGAGGAG 
TACGAGTGCC AGTGCTGCAG CGGATTCGCA GGACCACACT GCGAGGAGAT AGACGCCTGC 
AATCCGAGTC CTTGCACCAA CAACGGCATC TGCGTGGACC TCTCGCAGGG CCACGAGGGC 
AACTCGTACC AGTGCCTGTG TCCGTACGGA TACGCGGGCA AGAACTGTCA ATACGAGTCG 
GATCCCTGCA ATCCCGCCGA GTGCATGAAT GGCGGCAGCT GTGTGGGCAA CTCGACGCAC 
TTTCGCTGCG ACTGTGCTCC CGGGTTCACA GGACCTCTTT GCCAGCACAG CCTGAATGAG 
TGCGAGTCCT CGCCGTGTGT TCACGGCATC TGCGTCGACC AGGAGGACGG ATTCCGCTGC 
TTCTGCCAGC CAGGATTCGC CGGCGAGCTG TGCAACTTCG AGTATAATGA ATGCGAATCG 
AATCCGTGTC AGAATGGCGG CGAGTGCATC GATCACATTG GCAGCTACGA GTGTCGCTGC 
ACGAAGGGAT TCAGCGGCAA CCGCTGCCAA ATTAAGGTTG ATTTCTGCGC CAACAAGCCC 
TGCCCCGAAG GACATCGCTG TATTGATCAT GGAAATGATT TCAGCTGCGA ATGCCCAGGA 
GGTCGCAACG GACCGGATTG TAATCAAATG CCACGAACGT ACTTCCAGCA ATGCAACGTG 
AATCCGTGCA CCCATGGCGG CACTTGTTGG TCCAGCGGCG ATAGCTTCTA CTGCGCCTGT 
CGTCCCGGCT ACACAGGAAC CATGTGCGAA GATGAGTTCG TGGTGGAGAC GGTCGTTAGT 
TCCAGCGAAT TTATTGTGGA CGATGCGAAC GCCAGAAATT TTAATGACAA AACTTTCGGT 
TCATCGGTTG TGCTTAAGAG TCCCATTGAA TTGCATAATG CATATTTTGC GGCCGGAGTC 
CTGGCAGCCG CAATTTTCAT CGTTGCCGTT GTGGTCACCA TTTGTCACTG CAAGGTCAAT 
CAGACTTATC GAAAATTCTC CACACGTTCC ACCTCGTTTA TACCCATACT GGGCTTCAGT 
CGCCGCCCGA AAGTGCAGGG CAAACTGAAC AAACACTGGC TAAGTGGCAA GGGGGCCACC 
GCGACCAGCA GCACCAATAT TGTGGCGCCC CCGGGTGGAC CGAGGGCCGG AGGAGCAGCA 
GGGCCGCAGC AGGTAACCCG ACATCTGCCT GGACAGCCTC AGACGCAGAC CACACTGGGT 
GGCCAGCTGC AGGAGCGACC TTTTCTGCGG CACCTGGCAA TGAATCTGGA GAACGACATG 
TACTACACGG TGGACTTTAG CGAGAACTCA CAGCACTCGC CGTTGATACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wry cDNA ORF clone, Drosophila melanogaster(fruit fly)

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