Nle cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nle gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nle cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa13368 NM_057946.4
Latest version!
Drosophila melanogaster notchless (Nle), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa13368
    Clone ID Related Accession (Same CDS sequence) NM_057946.4
    Accession Version NM_057946.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product notchless
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057946.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTGGCCA AAAAACAGAA AATGCAGGAG ACGGACACGG AGCAAGAGGC CACGCCACAT 
    ACGATACAGG CGCGCCTCGT TTCGGACACG GGCGAGGAAG CGGGCCCGCC AATCGACCTG
    CCGGCAGGAA TCACTACCCA GCAATTGGGT CTGATTTGCA ACGCGCTGCT GAAAAACGAG
    GAAGCCACTC CATATTTGTT TTTCGTGGGC GAGGATGAGA TCAAGAAGAG CCTGGAGGAC
    ACGTTGGACT TGGCGTCAGT GGACACCGAA AACGTGATCG ATATTGTGTA TCAGCCACAG
    GCGGTTTTCA AAGTGCGCCC AGTGACAAGA TGCACGAGTT CCATGCCGGG ACACGCCGAG
    GCTGTGGTTT CGCTGAATTT CAGCCCGGAT GGTGCTCATC TCGCCAGTGG AAGTGGCGAC
    ACCACAGTGC GATTGTGGGA TCTTAACACA GAGACACCGC ACTTCACCTG CACAGGTCAT
    AAGCAGTGGG TTCTGTGCGT ATCCTGGGCT CCGGATGGCA AACGGTTGGC CAGCGGTTGC
    AAAGCGGGCT CTATAATCAT CTGGGACCCG GAGACGGGTC AGCAGAAGGG GCGACCCTTG
    AGTGGGCACA AGAAACACAT CAACTGCCTC GCCTGGGAAC CGTATCATCG CGATCCGGAG
    TGCAGGAAAC TTGCTTCCGC CAGTGGAGAC GGGGACTGCC GGATTTGGGA CGTGAAATTG
    GGCCAGTGCC TTATGAACAT TGCCGGACAC ACAAATGCTG TGACAGCAGT GAGATGGGGT
    GGAGCGGGCC TTATTTATAC ATCCTCCAAA GATCGCACAG TGAAGATGTG GCGAGCAGCT
    GATGGAATCT TGTGCCGGAC GTTCTCTGGC CACGCTCACT GGGTAAACAA CATTGCGCTG
    AGCACCGACT ACGTCCTGCG CACTGGTCCA TTCCATCCGG TGAAGGATCG CTCCAAGAGC
    CACCTCAGTT TGAGCACTGA GGAATTGCAG GAATCTGCCT TGAAGCGCTA CCAGGCCGTG
    TGCCCTGACG AGGTGGAGTC GCTGGTTTCC TGTTCGGATG ACAACACCCT CTATCTGTGG
    CGGAACAACC AGAACAAGTG CGTTGAGCGC ATGACAGGGC ACCAGAACGT GGTCAACGAC
    GTGAAATATT CGCCGGATGT AAAGCTAATT GCGTCTGCTT CATTTGACAA GTCAGTGCGT
    CTGTGGCGAG CCAGCGATGG TCAGTACATG GCCACCTTCC GGGGTCATGT GCAGGCTGTT
    TACACGGTTG CCTGGTCCGC GGACTCCCGC TTGATTGTTT CCGGCAGCAA AGACTCAACT
    CTAAAAGTAT GGAGTGTGCA GACGAAGAAA CTGGCACAGG AGCTGCCTGG ACATGCGGAT
    GAGGTGTTCG GAGTGGACTG GGCGCCCGAT GGCTCTAGAG TTGCCTCTGG TGGCAAGGAC
    AAAGTTATAA AGCTATGGGC TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477294.2
    CDS58..1524
    Translation

    Target ORF information:

    RefSeq Version NM_057946.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster notchless (Nle), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057946.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTTGGCCA AAAAACAGAA AATGCAGGAG ACGGACACGG AGCAAGAGGC CACGCCACAT 
    ACGATACAGG CGCGCCTCGT TTCGGACACG GGCGAGGAAG CGGGCCCGCC AATCGACCTG
    CCGGCAGGAA TCACTACCCA GCAATTGGGT CTGATTTGCA ACGCGCTGCT GAAAAACGAG
    GAAGCCACTC CATATTTGTT TTTCGTGGGC GAGGATGAGA TCAAGAAGAG CCTGGAGGAC
    ACGTTGGACT TGGCGTCAGT GGACACCGAA AACGTGATCG ATATTGTGTA TCAGCCACAG
    GCGGTTTTCA AAGTGCGCCC AGTGACAAGA TGCACGAGTT CCATGCCGGG ACACGCCGAG
    GCTGTGGTTT CGCTGAATTT CAGCCCGGAT GGTGCTCATC TCGCCAGTGG AAGTGGCGAC
    ACCACAGTGC GATTGTGGGA TCTTAACACA GAGACACCGC ACTTCACCTG CACAGGTCAT
    AAGCAGTGGG TTCTGTGCGT ATCCTGGGCT CCGGATGGCA AACGGTTGGC CAGCGGTTGC
    AAAGCGGGCT CTATAATCAT CTGGGACCCG GAGACGGGTC AGCAGAAGGG GCGACCCTTG
    AGTGGGCACA AGAAACACAT CAACTGCCTC GCCTGGGAAC CGTATCATCG CGATCCGGAG
    TGCAGGAAAC TTGCTTCCGC CAGTGGAGAC GGGGACTGCC GGATTTGGGA CGTGAAATTG
    GGCCAGTGCC TTATGAACAT TGCCGGACAC ACAAATGCTG TGACAGCAGT GAGATGGGGT
    GGAGCGGGCC TTATTTATAC ATCCTCCAAA GATCGCACAG TGAAGATGTG GCGAGCAGCT
    GATGGAATCT TGTGCCGGAC GTTCTCTGGC CACGCTCACT GGGTAAACAA CATTGCGCTG
    AGCACCGACT ACGTCCTGCG CACTGGTCCA TTCCATCCGG TGAAGGATCG CTCCAAGAGC
    CACCTCAGTT TGAGCACTGA GGAATTGCAG GAATCTGCCT TGAAGCGCTA CCAGGCCGTG
    TGCCCTGACG AGGTGGAGTC GCTGGTTTCC TGTTCGGATG ACAACACCCT CTATCTGTGG
    CGGAACAACC AGAACAAGTG CGTTGAGCGC ATGACAGGGC ACCAGAACGT GGTCAACGAC
    GTGAAATATT CGCCGGATGT AAAGCTAATT GCGTCTGCTT CATTTGACAA GTCAGTGCGT
    CTGTGGCGAG CCAGCGATGG TCAGTACATG GCCACCTTCC GGGGTCATGT GCAGGCTGTT
    TACACGGTTG CCTGGTCCGC GGACTCCCGC TTGATTGTTT CCGGCAGCAA AGACTCAACT
    CTAAAAGTAT GGAGTGTGCA GACGAAGAAA CTGGCACAGG AGCTGCCTGG ACATGCGGAT
    GAGGTGTTCG GAGTGGACTG GGCGCCCGAT GGCTCTAGAG TTGCCTCTGG TGGCAAGGAC
    AAAGTTATAA AGCTATGGGC TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.