nAChRalpha7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha7
Entrez Gene ID	32928
Full Name	nicotinic Acetylcholine Receptor alpha7
Synonyms	CG32538,CG8082,CG8109,CT24218,Da7,Dalpha7,Dalpha7 nAChR,Dalpha7-nAChR,Dmel\CG32538,gfA,gfa,jpl,nAChR,nAChR-Dalpha7,nAChR-gfa,nAcRalpha-18C,nAcRalpha-7,nAcRalpha18C,nAchRa7,nj507,nj520,nj522,nj54,nj75
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha7 Names CG32538 gene product from transcript CG32538-RD CG32538-PA CG32538-PD CG32538-PE CG32538-PF Dalpha7 Dalpha7 subunit of the acetylcholine receptor Dalpha7 subunit of the nicotinic acetylcholine receptor giant fiber A giant fibre A jumpless nAChRalpha7-PA nAChRalpha7-PD nAChRalpha7-PE nAChRalpha7-PF nicotinic acetylcholine receptor alpha 18C nicotinic acetylcholine receptor alpha7 non-jumper-507 non-jumper-520 non-jumper-522 non-jumper-54 non-jumper-75 shaker cognate L
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18C2-18C3\|1-62 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_206791.3	NP_996514.1	nicotinic acetylcholine receptor alpha7, isoform A
NM_167645.4	NP_728239.3	nicotinic acetylcholine receptor alpha7, isoform D
NM_001169324.2	NP_001162795.1	nicotinic acetylcholine receptor alpha7, isoform E
NM_001298507.1	NP_001285436.1	nicotinic acetylcholine receptor alpha7, isoform F

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100487782	XP_002935500.2
Danio rerio (zebrafish)	si:ch211-39a7.1	NP_001038562.1
Drosophila melanogaster (fruit fly)	gfA	NP_728239.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000962	XP_309153.3
Gallus gallus (chicken)	LOC396120	NP_990532.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of postsynaptic Dalpha7nAChRs caused a significant increase in the length of proximal dendrites, i.e., the cholinergic dendritic domain.
Title: Intra-neuronal Competition for Synaptic Partners Conserves the Amount of Dendritic Building Material.

essential role for the Dalpha7 nicotinic acetylcholine receptor in giant fiber-mediated escape in Drosophila
Title: The nicotinic acetylcholine receptor Dalpha7 is required for an escape behavior in Drosophila.

In addition to the formation of homomeric nAChR complexes, evidence has been obtained from both radioligand binding and co-immunoprecipitation studies for the co-assembly of Dalpha6 and Dalpha7 into heteromeric cell surface complexes
Title: Molecular characterization of Dalpha6 and Dalpha7 nicotinic acetylcholine receptor subunits from Drosophila: formation of a high-affinity alpha-bungarotoxin binding site revealed by expression of subunit chimeras.

The following nAChRalpha7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02114	NM_001298507.1 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant F (nAChRalpha7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02114	NM_167645.4 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant D (nAChRalpha7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02114	NM_206791.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant A (nAChRalpha7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02941	NM_001169324.2 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant E (nAChRalpha7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha7 ( NM_206791.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996514.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant A (nAChRalpha7), mRNA.

Price & Availability↑

CloneID	OFa02114
Clone ID Related Accession (Same CDS sequence)	NM_206791.3 , NM_167645.4 , NM_001298507.1
Accession Version	NM_206791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha7, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206791.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996514.1
CDS	1305..2987
Translation	MKKPSRSRPDNPSCPGQDRTRSMSFPQPHSLPEATANGGRMLVYGLGLLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISSFITNGEWDLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEMSEWIRVIFLYWLPCILRMQRPGQVGYECPPPPSSSSSSASGEKKQQIQNVELKERSSKSLLANVLDIDDDFRCNHRCASATLPHQPTYYRTMYRQGDDGSVGPVGPAGPVVDGRLHEAISHTCLTSSAEYELALILKELRWITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAPHFIFP

Target ORF information:

RefSeq Version	NM_206791.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant A (nAChRalpha7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206791.3

ORF Insert Sequence:

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha7 ( NM_167645.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728239.3 Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant D (nAChRalpha7), mRNA.

Price & Availability↑

CloneID	OFa02114
Clone ID Related Accession (Same CDS sequence)	NM_206791.3 , NM_167645.4 , NM_001298507.1
Accession Version	NM_167645.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha7, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167645.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728239.3
CDS	1203..2885
Translation	MKKPSRSRPDNPSCPGQDRTRSMSFPQPHSLPEATANGGRMLVYGLGLLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISSFITNGEWDLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEMSEWIRVIFLYWLPCILRMQRPGQVGYECPPPPSSSSSSASGEKKQQIQNVELKERSSKSLLANVLDIDDDFRCNHRCASATLPHQPTYYRTMYRQGDDGSVGPVGPAGPVVDGRLHEAISHTCLTSSAEYELALILKELRWITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAPHFIFP

Target ORF information:

RefSeq Version	NM_167645.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant D (nAChRalpha7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167645.4

ORF Insert Sequence:

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha7 ( NM_001298507.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285436.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant F (nAChRalpha7), mRNA.

Price & Availability↑

CloneID	OFa02114
Clone ID Related Accession (Same CDS sequence)	NM_206791.3 , NM_167645.4 , NM_001298507.1
Accession Version	NM_001298507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha7, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285436.1
CDS	1288..2970
Translation	MKKPSRSRPDNPSCPGQDRTRSMSFPQPHSLPEATANGGRMLVYGLGLLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISSFITNGEWDLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEMSEWIRVIFLYWLPCILRMQRPGQVGYECPPPPSSSSSSASGEKKQQIQNVELKERSSKSLLANVLDIDDDFRCNHRCASATLPHQPTYYRTMYRQGDDGSVGPVGPAGPVVDGRLHEAISHTCLTSSAEYELALILKELRWITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAPHFIFP

Target ORF information:

RefSeq Version	NM_001298507.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant F (nAChRalpha7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298507.1

ORF Insert Sequence:

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTTTCCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha7 ( NM_001169324.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162795.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant E (nAChRalpha7), mRNA.

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CloneID	OFa02941
Clone ID Related Accession (Same CDS sequence)	NM_001169324.2
Accession Version	NM_001169324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha7, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169324.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTGTGAGT 
GGAGTCCGTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162795.1
CDS	1305..2999
Translation	MKKPSRSRPDNPSCPGQDRTRSMSFPQPHSLPEATANGGRMLVYGLGLLIMIPACAAGPHEKRLLHALLDNYNSLERPVVNESDPLQLSFGLTLMQIIDVDEKNQLLITNIWLKLEWNDMNLRWNSSEFGGVRDLRIPPHRLWKPDVLMYNSADEGFDGTYATNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDEAGGDISSFITNGEWDLLGVPGKRNEIYYNCCPEPYIDITFAILIRRKTLYYFFNLIVPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNPDTHEMSEWIRVIFLYWLPCILRMQRPGQVGYECPPPPSSSSSSASGEKKQQIQNVELKERSSKSLLANVLDIDDDFRCNHRCASATLPHQPTYYRTMYRQGDDGSVGPVGPAGPVVDGRLHEAISHTCLTSSAEYELALILKELRWITEQLKKEDETSDITRDWKFAAMVVDRLCLIIFTLFTIIATLAVLFSAPHFIVSGVRG

Target ORF information:

RefSeq Version	NM_001169324.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha7, transcript variant E (nAChRalpha7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169324.2

ORF Insert Sequence:

ATGAAGAAGC CATCACGCAG TCGGCCAGAC AATCCATCCT GCCCTGGTCA GGATAGAACG 
AGATCCATGA GCTTCCCACA ACCGCACTCA TTGCCGGAGG CCACTGCAAA CGGTGGCAGA 
ATGCTGGTCT ATGGCCTGGG ACTTTTAATT ATGATACCGG CTTGTGCGGC TGGACCCCAT 
GAGAAGCGGC TACTCCACGC CCTTCTGGAC AACTACAACA GCCTGGAGCG TCCGGTGGTC 
AATGAATCCG ATCCATTGCA ACTGAGCTTC GGACTAACAC TCATGCAGAT TATCGATGTG 
GACGAAAAGA ATCAACTGCT TATAACGAAT ATTTGGCTCA AATTGGAATG GAACGATATG 
AATCTTCGAT GGAATTCGAG TGAGTTCGGT GGTGTGCGGG ATCTGCGAAT TCCGCCACAT 
CGCCTATGGA AACCGGATGT ACTGATGTAC AACAGTGCCG ACGAGGGCTT CGATGGAACG 
TACGCCACAA ATGTGGTGGT TCGCAATAAT GGGAGCTGTC TGTACGTACC GCCAGGTATA 
TTTAAGTCAA CGTGTAAGAT CGACATTACG TGGTTTCCAT TCGACGATCA GAGATGTGAA 
ATGAAATTTG GTTCGTGGAC CTACGATGGG TTTCAGTTGG ACCTGCAGTT GCAGGACGAA 
GCTGGTGGCG ACATTTCTAG CTTTATAACC AATGGCGAAT GGGACTTGTT AGGTGTGCCC 
GGTAAACGAA ATGAAATCTA CTATAATTGC TGCCCAGAAC CTTATATTGA CATAACATTC 
GCCATTTTGA TAAGGCGCAA AACGTTGTAC TATTTTTTCA ATCTGATTGT GCCGTGCGTA 
CTGATCGCCT CCATGGCACT GCTAGGGTTT ACACTGCCAC CAGATTCTGG TGAAAAGCTT 
TCGCTTGGAG TTACAATTCT ATTATCGCTT ACAGTCTTCC TCAACATGGT GGCCGAAACA 
ATGCCGGCGA CCTCCGATGC GGTACCGCTG CTCGGAACTT ATTTCAATTG CATTATGTTT 
ATGGTGGCCT CATCAGTTGT GTCAACCATA CTTATCCTCA ATTATCATCA TAGAAATCCA 
GATACGCATG AAATGAGTGA ATGGATAAGA GTAATATTCC TTTATTGGTT ACCTTGCATA 
TTGCGCATGC AAAGACCCGG ACAGGTTGGC TACGAATGTC CGCCGCCGCC CTCTTCTTCG 
AGTTCCTCCG CATCCGGCGA GAAGAAGCAA CAGATCCAAA ACGTTGAGCT CAAGGAGAGA 
TCCTCCAAGT CTCTGCTGGC CAATGTGCTC GATATAGACG ATGATTTCCG ATGCAATCAT 
CGATGTGCCA GCGCGACTTT GCCCCACCAG CCCACATATT ACAGGACGAT GTACAGGCAA 
GGGGATGACG GCAGCGTGGG ACCCGTGGGA CCAGCTGGTC CAGTTGTGGA CGGGCGTTTG 
CACGAGGCCA TTTCCCACAC CTGTCTGACA TCCTCTGCGG AGTACGAACT GGCGCTGATA 
CTCAAGGAGC TGCGTTGGAT AACAGAACAG CTCAAAAAAG AGGACGAAAC AAGCGACATT 
ACGCGAGATT GGAAATTTGC TGCCATGGTC GTCGATCGTT TGTGCCTTAT TATTTTCACC 
TTGTTTACTA TTATAGCAAC CCTCGCTGTA CTCTTCTCAG CGCCGCATTT CATTGTGAGT 
GGAGTCCGTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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