out cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	out
Entrez Gene ID	32926
Full Name	outsiders
Synonyms	BcDNA:LD28120,CG8062,Dmel\CG8062
General protein information	Preferred Names outsiders Names CG8062 gene product from transcript CG8062-RB CG8062-PA CG8062-PB out-PA out-PB outsider
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18C2-18C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_133150.3	NP_573378.1	outsiders, isoform A
NM_001298506.1	NP_001285435.1	outsiders, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14196	NP_573367.1
Drosophila melanogaster (fruit fly)	CG8051	NP_573377.1
Drosophila melanogaster (fruit fly)	out	NP_573378.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000718	XP_311256.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings inform models in which signaling between p53 and the Outsiders (out) monocarboxylate transporter are integrated to regulate programmed cell death decisions.
Title: Programmed cell death of primordial germ cells in Drosophila is regulated by p53 and the Outsiders monocarboxylate transporter.

The following out gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the out cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02113	NM_133150.3 Latest version!	Drosophila melanogaster outsiders (out), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02113	NM_001298506.1 Latest version!	Drosophila melanogaster outsiders (out), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

out ( NM_133150.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573378.1 Drosophila melanogaster outsiders (out), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02113
Clone ID Related Accession (Same CDS sequence)	NM_133150.3 , NM_001298506.1
Accession Version	NM_133150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	outsiders, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_133150.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAT CCAGCTTAAC GGAGAAGAAT CACACCCAAG TGTACAATGA CACAACGAAG 
CCAAAGAAGC CCAAGCGTCG CGACAAGAGC GATCTGGGTC CGGATTTTGT GGCGCCCGAC 
GGGGGATGGG GCTGGGTGGT CTGTCTGGCA GCCGGTTTGA ATAACTTCTT CCTTTTCCCG 
GCTCTGCAGC AGTATGGCCT GATCTACAGG GTGCGGATGC AATCGCTGGG CTTCGATGCC 
AAGCAAACCA CGACCATTGT GAACGTGGTG ATGGCAATCT CCTCGCTAGT GGGCATTGTC 
AATGGCGCCA TGTTCCGGCG GTTCACATTC CGTCAGGTGG CCCTGACCGG CACCAGTCTG 
GCCTTCCTGG GCGTCTTCTT GTCGGCCTTT TGCACCACCT TCTGGCAGTA TATCATCTGT 
CTGTCGGCGA TATTTGGTAT CGGTTTGGGT CTTGCCATGG CCGCCACCTC ACTGGCGGTC 
AATACGTACT TCAAGCTGAA GAGGCGTCGT GCCACGGGTT TCTCGTGGAC GATCACTGGC 
CTGGGACCCA TATTCTTTCC GCAGGTGTCT ACCGTCCTGT TGGGCTACTA TGGTGCCCAG 
GGCACCATTC TGATCTATGC TGGAATCGCC ATGAATGCCA TTCTCTGCGC TTTGACCCTG 
CAACCCGTAC TTTGGCATGT GAAGAAGCCC GAAAAACCAG TTCACAATAC AATCGAGGGA 
ATTGCCGAGA CGGAGAAGCT GCAACCAGAA CTTTTGGAGG CCAATGGCAA TCTGTTGTCG 
CCCAGCAATG ATCCGTGGAA GGATTACGAG TGCAAGTACT GTCAGTATCA GAAACGTTCT 
AAGCGAGGCC TTTTCTCGTC CCAATACCTC TTCAATGTCG ATGATCCCGA GAGGCCGGGG 
TACGAGATCA CCGAGCCAGG AACTCCCATG TTGGCTCGCG CCAATGACGG TTGGTTCGGG 
TCGAAGCTCT CCCTGACCTC GGAGAGCGCG GGAGGAGCTC GCTCTCGCAC TCGTCAAGCT 
CTGATGCGTC AGGTTTCATC CAGAAGTCGT GAGAATCTCG ATAGACTGGA ACAGAATCGT 
CATGACCAGG GTCCAGACAC TCCATCCGCT GCGGCCTTGT ACAAGCCAAA CTACTTCAAT 
CGGGAGCGCG AGGATCTCGA TCGCTATGCC AGCAAGACGA GCGTGTATTC TCGGCCTGGT 
CAGGATGAGC TGCTGCGCTG CACCTGTGCA GAGGACAAGG CACTGTTGCA AAAGACAGCC 
GAATCGCTTC AGGTGAACCT GTTCAATAAT TCGGCCGACA ACACGGAGGC CGAGGAGGAG 
GCCAAAAGGC GCATGACCTT CTTCCAGAAG GTGAGCAAGT TCTTTGATTT GGATCTACTG 
CGGGACTTTA CATTTGTCAA CCTGGCCGTT GGCATGAGCA TCATGATGTT TGGCGAGATG 
AACTTCTCGG TGCTGACACC ATTCATTCTG AACAGCTTTG GGTACACGGA TACACAGATC 
TCGCTGGTGA TGTCGCTGCT GGCCTGCATG GATATATCGG TGAGATTTCT CGCTCCGCTC 
GCCTTGGAGA AGGTGAAGCT GGATAACCGT GTGCTGTTTG CCTTCGGCAT CCTATGCATC 
GCCGTGGGCC GCGTGGTTGT GGCCTTTACC GACTCCTACG AGATCATGAT CGGCGTCTTC 
CTGCTGATCG GCTTTGGCAA GGCATTCCGC ACCATCTTCT CACCGCTGAT AATACCCAGT 
TACGTTCCGC TCAACCGCTT GCCAGCTGCA TCTGGTCTGC AGCTCATCTT CAACACAATA 
TTCTCGTTCG CCATGGGCCC AATTCTTGGT ATCTTGACGG AGGCCTACGG TTATGCAGCC 
ACCATTCATA CCATCAATGC ACTCACCCTT TTGGCTTTAC TCCTTTGGTT AGCTGAGTCC 
GTGGTGCGCC GCATTTTGGG TATACCCTCC AAAGGATTGG GCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573378.1
CDS	469..2436
Translation	MEKSSLTEKNHTQVYNDTTKPKKPKRRDKSDLGPDFVAPDGGWGWVVCLAAGLNNFFLFPALQQYGLIYRVRMQSLGFDAKQTTTIVNVVMAISSLVGIVNGAMFRRFTFRQVALTGTSLAFLGVFLSAFCTTFWQYIICLSAIFGIGLGLAMAATSLAVNTYFKLKRRRATGFSWTITGLGPIFFPQVSTVLLGYYGAQGTILIYAGIAMNAILCALTLQPVLWHVKKPEKPVHNTIEGIAETEKLQPELLEANGNLLSPSNDPWKDYECKYCQYQKRSKRGLFSSQYLFNVDDPERPGYEITEPGTPMLARANDGWFGSKLSLTSESAGGARSRTRQALMRQVSSRSRENLDRLEQNRHDQGPDTPSAAALYKPNYFNREREDLDRYASKTSVYSRPGQDELLRCTCAEDKALLQKTAESLQVNLFNNSADNTEAEEEAKRRMTFFQKVSKFFDLDLLRDFTFVNLAVGMSIMMFGEMNFSVLTPFILNSFGYTDTQISLVMSLLACMDISVRFLAPLALEKVKLDNRVLFAFGILCIAVGRVVVAFTDSYEIMIGVFLLIGFGKAFRTIFSPLIIPSYVPLNRLPAASGLQLIFNTIFSFAMGPILGILTEAYGYAATIHTINALTLLALLLWLAESVVRRILGIPSKGLGQ

Target ORF information:

RefSeq Version	NM_133150.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster outsiders (out), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133150.3

ORF Insert Sequence:

ATGGAAAAAT CCAGCTTAAC GGAGAAGAAT CACACCCAAG TGTACAATGA CACAACGAAG 
CCAAAGAAGC CCAAGCGTCG CGACAAGAGC GATCTGGGTC CGGATTTTGT GGCGCCCGAC 
GGGGGATGGG GCTGGGTGGT CTGTCTGGCA GCCGGTTTGA ATAACTTCTT CCTTTTCCCG 
GCTCTGCAGC AGTATGGCCT GATCTACAGG GTGCGGATGC AATCGCTGGG CTTCGATGCC 
AAGCAAACCA CGACCATTGT GAACGTGGTG ATGGCAATCT CCTCGCTAGT GGGCATTGTC 
AATGGCGCCA TGTTCCGGCG GTTCACATTC CGTCAGGTGG CCCTGACCGG CACCAGTCTG 
GCCTTCCTGG GCGTCTTCTT GTCGGCCTTT TGCACCACCT TCTGGCAGTA TATCATCTGT 
CTGTCGGCGA TATTTGGTAT CGGTTTGGGT CTTGCCATGG CCGCCACCTC ACTGGCGGTC 
AATACGTACT TCAAGCTGAA GAGGCGTCGT GCCACGGGTT TCTCGTGGAC GATCACTGGC 
CTGGGACCCA TATTCTTTCC GCAGGTGTCT ACCGTCCTGT TGGGCTACTA TGGTGCCCAG 
GGCACCATTC TGATCTATGC TGGAATCGCC ATGAATGCCA TTCTCTGCGC TTTGACCCTG 
CAACCCGTAC TTTGGCATGT GAAGAAGCCC GAAAAACCAG TTCACAATAC AATCGAGGGA 
ATTGCCGAGA CGGAGAAGCT GCAACCAGAA CTTTTGGAGG CCAATGGCAA TCTGTTGTCG 
CCCAGCAATG ATCCGTGGAA GGATTACGAG TGCAAGTACT GTCAGTATCA GAAACGTTCT 
AAGCGAGGCC TTTTCTCGTC CCAATACCTC TTCAATGTCG ATGATCCCGA GAGGCCGGGG 
TACGAGATCA CCGAGCCAGG AACTCCCATG TTGGCTCGCG CCAATGACGG TTGGTTCGGG 
TCGAAGCTCT CCCTGACCTC GGAGAGCGCG GGAGGAGCTC GCTCTCGCAC TCGTCAAGCT 
CTGATGCGTC AGGTTTCATC CAGAAGTCGT GAGAATCTCG ATAGACTGGA ACAGAATCGT 
CATGACCAGG GTCCAGACAC TCCATCCGCT GCGGCCTTGT ACAAGCCAAA CTACTTCAAT 
CGGGAGCGCG AGGATCTCGA TCGCTATGCC AGCAAGACGA GCGTGTATTC TCGGCCTGGT 
CAGGATGAGC TGCTGCGCTG CACCTGTGCA GAGGACAAGG CACTGTTGCA AAAGACAGCC 
GAATCGCTTC AGGTGAACCT GTTCAATAAT TCGGCCGACA ACACGGAGGC CGAGGAGGAG 
GCCAAAAGGC GCATGACCTT CTTCCAGAAG GTGAGCAAGT TCTTTGATTT GGATCTACTG 
CGGGACTTTA CATTTGTCAA CCTGGCCGTT GGCATGAGCA TCATGATGTT TGGCGAGATG 
AACTTCTCGG TGCTGACACC ATTCATTCTG AACAGCTTTG GGTACACGGA TACACAGATC 
TCGCTGGTGA TGTCGCTGCT GGCCTGCATG GATATATCGG TGAGATTTCT CGCTCCGCTC 
GCCTTGGAGA AGGTGAAGCT GGATAACCGT GTGCTGTTTG CCTTCGGCAT CCTATGCATC 
GCCGTGGGCC GCGTGGTTGT GGCCTTTACC GACTCCTACG AGATCATGAT CGGCGTCTTC 
CTGCTGATCG GCTTTGGCAA GGCATTCCGC ACCATCTTCT CACCGCTGAT AATACCCAGT 
TACGTTCCGC TCAACCGCTT GCCAGCTGCA TCTGGTCTGC AGCTCATCTT CAACACAATA 
TTCTCGTTCG CCATGGGCCC AATTCTTGGT ATCTTGACGG AGGCCTACGG TTATGCAGCC 
ACCATTCATA CCATCAATGC ACTCACCCTT TTGGCTTTAC TCCTTTGGTT AGCTGAGTCC 
GTGGTGCGCC GCATTTTGGG TATACCCTCC AAAGGATTGG GCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

out ( NM_001298506.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285435.1 Drosophila melanogaster outsiders (out), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02113
Clone ID Related Accession (Same CDS sequence)	NM_133150.3 , NM_001298506.1
Accession Version	NM_001298506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	outsiders, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAAT CCAGCTTAAC GGAGAAGAAT CACACCCAAG TGTACAATGA CACAACGAAG 
CCAAAGAAGC CCAAGCGTCG CGACAAGAGC GATCTGGGTC CGGATTTTGT GGCGCCCGAC 
GGGGGATGGG GCTGGGTGGT CTGTCTGGCA GCCGGTTTGA ATAACTTCTT CCTTTTCCCG 
GCTCTGCAGC AGTATGGCCT GATCTACAGG GTGCGGATGC AATCGCTGGG CTTCGATGCC 
AAGCAAACCA CGACCATTGT GAACGTGGTG ATGGCAATCT CCTCGCTAGT GGGCATTGTC 
AATGGCGCCA TGTTCCGGCG GTTCACATTC CGTCAGGTGG CCCTGACCGG CACCAGTCTG 
GCCTTCCTGG GCGTCTTCTT GTCGGCCTTT TGCACCACCT TCTGGCAGTA TATCATCTGT 
CTGTCGGCGA TATTTGGTAT CGGTTTGGGT CTTGCCATGG CCGCCACCTC ACTGGCGGTC 
AATACGTACT TCAAGCTGAA GAGGCGTCGT GCCACGGGTT TCTCGTGGAC GATCACTGGC 
CTGGGACCCA TATTCTTTCC GCAGGTGTCT ACCGTCCTGT TGGGCTACTA TGGTGCCCAG 
GGCACCATTC TGATCTATGC TGGAATCGCC ATGAATGCCA TTCTCTGCGC TTTGACCCTG 
CAACCCGTAC TTTGGCATGT GAAGAAGCCC GAAAAACCAG TTCACAATAC AATCGAGGGA 
ATTGCCGAGA CGGAGAAGCT GCAACCAGAA CTTTTGGAGG CCAATGGCAA TCTGTTGTCG 
CCCAGCAATG ATCCGTGGAA GGATTACGAG TGCAAGTACT GTCAGTATCA GAAACGTTCT 
AAGCGAGGCC TTTTCTCGTC CCAATACCTC TTCAATGTCG ATGATCCCGA GAGGCCGGGG 
TACGAGATCA CCGAGCCAGG AACTCCCATG TTGGCTCGCG CCAATGACGG TTGGTTCGGG 
TCGAAGCTCT CCCTGACCTC GGAGAGCGCG GGAGGAGCTC GCTCTCGCAC TCGTCAAGCT 
CTGATGCGTC AGGTTTCATC CAGAAGTCGT GAGAATCTCG ATAGACTGGA ACAGAATCGT 
CATGACCAGG GTCCAGACAC TCCATCCGCT GCGGCCTTGT ACAAGCCAAA CTACTTCAAT 
CGGGAGCGCG AGGATCTCGA TCGCTATGCC AGCAAGACGA GCGTGTATTC TCGGCCTGGT 
CAGGATGAGC TGCTGCGCTG CACCTGTGCA GAGGACAAGG CACTGTTGCA AAAGACAGCC 
GAATCGCTTC AGGTGAACCT GTTCAATAAT TCGGCCGACA ACACGGAGGC CGAGGAGGAG 
GCCAAAAGGC GCATGACCTT CTTCCAGAAG GTGAGCAAGT TCTTTGATTT GGATCTACTG 
CGGGACTTTA CATTTGTCAA CCTGGCCGTT GGCATGAGCA TCATGATGTT TGGCGAGATG 
AACTTCTCGG TGCTGACACC ATTCATTCTG AACAGCTTTG GGTACACGGA TACACAGATC 
TCGCTGGTGA TGTCGCTGCT GGCCTGCATG GATATATCGG TGAGATTTCT CGCTCCGCTC 
GCCTTGGAGA AGGTGAAGCT GGATAACCGT GTGCTGTTTG CCTTCGGCAT CCTATGCATC 
GCCGTGGGCC GCGTGGTTGT GGCCTTTACC GACTCCTACG AGATCATGAT CGGCGTCTTC 
CTGCTGATCG GCTTTGGCAA GGCATTCCGC ACCATCTTCT CACCGCTGAT AATACCCAGT 
TACGTTCCGC TCAACCGCTT GCCAGCTGCA TCTGGTCTGC AGCTCATCTT CAACACAATA 
TTCTCGTTCG CCATGGGCCC AATTCTTGGT ATCTTGACGG AGGCCTACGG TTATGCAGCC 
ACCATTCATA CCATCAATGC ACTCACCCTT TTGGCTTTAC TCCTTTGGTT AGCTGAGTCC 
GTGGTGCGCC GCATTTTGGG TATACCCTCC AAAGGATTGG GCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285435.1
CDS	469..2436
Translation	MEKSSLTEKNHTQVYNDTTKPKKPKRRDKSDLGPDFVAPDGGWGWVVCLAAGLNNFFLFPALQQYGLIYRVRMQSLGFDAKQTTTIVNVVMAISSLVGIVNGAMFRRFTFRQVALTGTSLAFLGVFLSAFCTTFWQYIICLSAIFGIGLGLAMAATSLAVNTYFKLKRRRATGFSWTITGLGPIFFPQVSTVLLGYYGAQGTILIYAGIAMNAILCALTLQPVLWHVKKPEKPVHNTIEGIAETEKLQPELLEANGNLLSPSNDPWKDYECKYCQYQKRSKRGLFSSQYLFNVDDPERPGYEITEPGTPMLARANDGWFGSKLSLTSESAGGARSRTRQALMRQVSSRSRENLDRLEQNRHDQGPDTPSAAALYKPNYFNREREDLDRYASKTSVYSRPGQDELLRCTCAEDKALLQKTAESLQVNLFNNSADNTEAEEEAKRRMTFFQKVSKFFDLDLLRDFTFVNLAVGMSIMMFGEMNFSVLTPFILNSFGYTDTQISLVMSLLACMDISVRFLAPLALEKVKLDNRVLFAFGILCIAVGRVVVAFTDSYEIMIGVFLLIGFGKAFRTIFSPLIIPSYVPLNRLPAASGLQLIFNTIFSFAMGPILGILTEAYGYAATIHTINALTLLALLLWLAESVVRRILGIPSKGLGQ

Target ORF information:

RefSeq Version	NM_001298506.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster outsiders (out), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298506.1

ORF Insert Sequence:

ATGGAAAAAT CCAGCTTAAC GGAGAAGAAT CACACCCAAG TGTACAATGA CACAACGAAG 
CCAAAGAAGC CCAAGCGTCG CGACAAGAGC GATCTGGGTC CGGATTTTGT GGCGCCCGAC 
GGGGGATGGG GCTGGGTGGT CTGTCTGGCA GCCGGTTTGA ATAACTTCTT CCTTTTCCCG 
GCTCTGCAGC AGTATGGCCT GATCTACAGG GTGCGGATGC AATCGCTGGG CTTCGATGCC 
AAGCAAACCA CGACCATTGT GAACGTGGTG ATGGCAATCT CCTCGCTAGT GGGCATTGTC 
AATGGCGCCA TGTTCCGGCG GTTCACATTC CGTCAGGTGG CCCTGACCGG CACCAGTCTG 
GCCTTCCTGG GCGTCTTCTT GTCGGCCTTT TGCACCACCT TCTGGCAGTA TATCATCTGT 
CTGTCGGCGA TATTTGGTAT CGGTTTGGGT CTTGCCATGG CCGCCACCTC ACTGGCGGTC 
AATACGTACT TCAAGCTGAA GAGGCGTCGT GCCACGGGTT TCTCGTGGAC GATCACTGGC 
CTGGGACCCA TATTCTTTCC GCAGGTGTCT ACCGTCCTGT TGGGCTACTA TGGTGCCCAG 
GGCACCATTC TGATCTATGC TGGAATCGCC ATGAATGCCA TTCTCTGCGC TTTGACCCTG 
CAACCCGTAC TTTGGCATGT GAAGAAGCCC GAAAAACCAG TTCACAATAC AATCGAGGGA 
ATTGCCGAGA CGGAGAAGCT GCAACCAGAA CTTTTGGAGG CCAATGGCAA TCTGTTGTCG 
CCCAGCAATG ATCCGTGGAA GGATTACGAG TGCAAGTACT GTCAGTATCA GAAACGTTCT 
AAGCGAGGCC TTTTCTCGTC CCAATACCTC TTCAATGTCG ATGATCCCGA GAGGCCGGGG 
TACGAGATCA CCGAGCCAGG AACTCCCATG TTGGCTCGCG CCAATGACGG TTGGTTCGGG 
TCGAAGCTCT CCCTGACCTC GGAGAGCGCG GGAGGAGCTC GCTCTCGCAC TCGTCAAGCT 
CTGATGCGTC AGGTTTCATC CAGAAGTCGT GAGAATCTCG ATAGACTGGA ACAGAATCGT 
CATGACCAGG GTCCAGACAC TCCATCCGCT GCGGCCTTGT ACAAGCCAAA CTACTTCAAT 
CGGGAGCGCG AGGATCTCGA TCGCTATGCC AGCAAGACGA GCGTGTATTC TCGGCCTGGT 
CAGGATGAGC TGCTGCGCTG CACCTGTGCA GAGGACAAGG CACTGTTGCA AAAGACAGCC 
GAATCGCTTC AGGTGAACCT GTTCAATAAT TCGGCCGACA ACACGGAGGC CGAGGAGGAG 
GCCAAAAGGC GCATGACCTT CTTCCAGAAG GTGAGCAAGT TCTTTGATTT GGATCTACTG 
CGGGACTTTA CATTTGTCAA CCTGGCCGTT GGCATGAGCA TCATGATGTT TGGCGAGATG 
AACTTCTCGG TGCTGACACC ATTCATTCTG AACAGCTTTG GGTACACGGA TACACAGATC 
TCGCTGGTGA TGTCGCTGCT GGCCTGCATG GATATATCGG TGAGATTTCT CGCTCCGCTC 
GCCTTGGAGA AGGTGAAGCT GGATAACCGT GTGCTGTTTG CCTTCGGCAT CCTATGCATC 
GCCGTGGGCC GCGTGGTTGT GGCCTTTACC GACTCCTACG AGATCATGAT CGGCGTCTTC 
CTGCTGATCG GCTTTGGCAA GGCATTCCGC ACCATCTTCT CACCGCTGAT AATACCCAGT 
TACGTTCCGC TCAACCGCTT GCCAGCTGCA TCTGGTCTGC AGCTCATCTT CAACACAATA 
TTCTCGTTCG CCATGGGCCC AATTCTTGGT ATCTTGACGG AGGCCTACGG TTATGCAGCC 
ACCATTCATA CCATCAATGC ACTCACCCTT TTGGCTTTAC TCCTTTGGTT AGCTGAGTCC 
GTGGTGCGCC GCATTTTGGG TATACCCTCC AAAGGATTGG GCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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out cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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