RhoGAP16F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP16F
Entrez Gene ID	32790
Full Name	Rho GTPase activating protein at 16F
Synonyms	CG7122,Dmel\CG7122
General protein information	Preferred Names Rho GTPase activating protein at 16F Names CG7122 gene product from transcript CG7122-RF CG7122-PB CG7122-PC CG7122-PD CG7122-PE CG7122-PF RhoGAP16F-PB RhoGAP16F-PC RhoGAP16F-PD RhoGAP16F-PE RhoGAP16F-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16F6-16F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_133039.5	NP_573267.3	Rho GTPase activating protein at 16F, isoform D
NM_001298471.1	NP_001285400.1	Rho GTPase activating protein at 16F, isoform E
NM_001298472.1	NP_001285401.1	Rho GTPase activating protein at 16F, isoform F
NM_176755.3	NP_788928.2	Rho GTPase activating protein at 16F, isoform B
NM_001258801.2	NP_001245730.1	Rho GTPase activating protein at 16F, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP16F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP16F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01976	NM_133039.5 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01976	NM_176755.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01976	NM_001298472.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01976	NM_001258801.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21530	NM_001298471.1 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP16F ( NM_133039.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573267.3 Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01976
Clone ID Related Accession (Same CDS sequence)	NM_133039.5 , NM_001298472.1 , NM_176755.3 , NM_001258801.2
Accession Version	NM_133039.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 16F, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_133039.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573267.3
CDS	407..1888
Translation	MQTTTSQRPVTPGTPDSAHIVIGAAGFVAHERRQPGKTLMNCFRDRSTTSSSSSQAQHAYVRGHSVTSSTLFRLEGSGGSNCTLDKAGKQDTGRPEAVVLLRELKTRPNKLALLKSSSSKDLTRLFLDDSTNTAVLVSNTSIDVCSSNSSSGGSPAVGRSKSGRDRRNGLECCSSCSKRWHDLKQRMHTLEQDLLVQTTYSQELEQKVGEMSRQLTELLQERDRERDKDKARFAAAGGVAAPGKRTAHGSPNVRPSRLVAWMNLSNWFKSRPSVETLREQRIFFDEPCFDTELEMVLKHDQHRTVPRIVVDCCDLIEQKYRRSTQPIEGIYRQCGDYNKIQTLRFSIDANDYDSLRQPDVDIHTLTGVLKLFLREIKSPLVRVNEAKTFIGQPNQWLLTDLSAKLDSLKRLIRSLPESNRDTMDYIFGHFNRITKVPLQQISAETLSISVTPSIFHTVPQGVHMQDIQQLLREGETLADCVKLMIEYQGRIFE

Target ORF information:

RefSeq Version	NM_133039.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133039.5

ORF Insert Sequence:

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP16F ( NM_001298472.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285401.1 Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa01976
Clone ID Related Accession (Same CDS sequence)	NM_133039.5 , NM_001298472.1 , NM_176755.3 , NM_001258801.2
Accession Version	NM_001298472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 16F, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285401.1
CDS	1081..2562
Translation	MQTTTSQRPVTPGTPDSAHIVIGAAGFVAHERRQPGKTLMNCFRDRSTTSSSSSQAQHAYVRGHSVTSSTLFRLEGSGGSNCTLDKAGKQDTGRPEAVVLLRELKTRPNKLALLKSSSSKDLTRLFLDDSTNTAVLVSNTSIDVCSSNSSSGGSPAVGRSKSGRDRRNGLECCSSCSKRWHDLKQRMHTLEQDLLVQTTYSQELEQKVGEMSRQLTELLQERDRERDKDKARFAAAGGVAAPGKRTAHGSPNVRPSRLVAWMNLSNWFKSRPSVETLREQRIFFDEPCFDTELEMVLKHDQHRTVPRIVVDCCDLIEQKYRRSTQPIEGIYRQCGDYNKIQTLRFSIDANDYDSLRQPDVDIHTLTGVLKLFLREIKSPLVRVNEAKTFIGQPNQWLLTDLSAKLDSLKRLIRSLPESNRDTMDYIFGHFNRITKVPLQQISAETLSISVTPSIFHTVPQGVHMQDIQQLLREGETLADCVKLMIEYQGRIFE

Target ORF information:

RefSeq Version	NM_001298472.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298472.1

ORF Insert Sequence:

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP16F ( NM_176755.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788928.2 Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01976
Clone ID Related Accession (Same CDS sequence)	NM_133039.5 , NM_001298472.1 , NM_176755.3 , NM_001258801.2
Accession Version	NM_176755.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 16F, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_176755.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788928.2
CDS	606..2087
Translation	MQTTTSQRPVTPGTPDSAHIVIGAAGFVAHERRQPGKTLMNCFRDRSTTSSSSSQAQHAYVRGHSVTSSTLFRLEGSGGSNCTLDKAGKQDTGRPEAVVLLRELKTRPNKLALLKSSSSKDLTRLFLDDSTNTAVLVSNTSIDVCSSNSSSGGSPAVGRSKSGRDRRNGLECCSSCSKRWHDLKQRMHTLEQDLLVQTTYSQELEQKVGEMSRQLTELLQERDRERDKDKARFAAAGGVAAPGKRTAHGSPNVRPSRLVAWMNLSNWFKSRPSVETLREQRIFFDEPCFDTELEMVLKHDQHRTVPRIVVDCCDLIEQKYRRSTQPIEGIYRQCGDYNKIQTLRFSIDANDYDSLRQPDVDIHTLTGVLKLFLREIKSPLVRVNEAKTFIGQPNQWLLTDLSAKLDSLKRLIRSLPESNRDTMDYIFGHFNRITKVPLQQISAETLSISVTPSIFHTVPQGVHMQDIQQLLREGETLADCVKLMIEYQGRIFE

Target ORF information:

RefSeq Version	NM_176755.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176755.3

ORF Insert Sequence:

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP16F ( NM_001258801.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245730.1 Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant C, mRNA.

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CloneID	OFa01976
Clone ID Related Accession (Same CDS sequence)	NM_133039.5 , NM_001298472.1 , NM_176755.3 , NM_001258801.2
Accession Version	NM_001258801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 16F, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258801.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245730.1
CDS	346..1827
Translation	MQTTTSQRPVTPGTPDSAHIVIGAAGFVAHERRQPGKTLMNCFRDRSTTSSSSSQAQHAYVRGHSVTSSTLFRLEGSGGSNCTLDKAGKQDTGRPEAVVLLRELKTRPNKLALLKSSSSKDLTRLFLDDSTNTAVLVSNTSIDVCSSNSSSGGSPAVGRSKSGRDRRNGLECCSSCSKRWHDLKQRMHTLEQDLLVQTTYSQELEQKVGEMSRQLTELLQERDRERDKDKARFAAAGGVAAPGKRTAHGSPNVRPSRLVAWMNLSNWFKSRPSVETLREQRIFFDEPCFDTELEMVLKHDQHRTVPRIVVDCCDLIEQKYRRSTQPIEGIYRQCGDYNKIQTLRFSIDANDYDSLRQPDVDIHTLTGVLKLFLREIKSPLVRVNEAKTFIGQPNQWLLTDLSAKLDSLKRLIRSLPESNRDTMDYIFGHFNRITKVPLQQISAETLSISVTPSIFHTVPQGVHMQDIQQLLREGETLADCVKLMIEYQGRIFE

Target ORF information:

RefSeq Version	NM_001258801.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258801.2

ORF Insert Sequence:

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP16F ( NM_001298471.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285400.1 Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant E, mRNA.

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CloneID	OFa21530
Clone ID Related Accession (Same CDS sequence)	NM_001298471.1
Accession Version	NM_001298471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 16F, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGACC GCTTACGTCT CATCCAAACC 
GCTCACCTAC TGTGCCGTTG TGTCGAACGC TTTGCTAACC ACTCGAAATC CGAAATCCTC 
AACCCGAAAC CGAACACCCC ACTGTACCTG CCACACCTCT ATACCCATTC CCGATTCGCT 
CCCGAATCTG TTTCCAATCC CGATCACATC GTCTACAGAT GCACCGCCGA TCGCCGCCGC 
CTGAGCATGC GCAACCTAAA GAAGACTCTG TCGCAGCCGG ACCTGCTCTT TCACAATCTA 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285400.1
CDS	346..2091
Translation	MQTTTSQRPVTPGTPDSAHIVIGAAGFVAHERRQPGKTLMNCFRDRSTTSSSSSQAQHAYVRGHSVTSSTLFRLEGSGGSNCTLDKAGKQDTGRPEAVVLLRELKTRPNKLALLKSSSSKDLTRLFLDDSTNTAVLVSNTSIDVCSSNSSSGGSPAVGRSKSGRDRRNGLECCSSCSKRWHDLKQRMHTLEQDLLVQTTYSQELEQKVGEMSRQLTELLQERDRERDKDKARFAAAGGVAAPGKRTAHGSPNVRPSRLVAWMNLSNWFKSRPSVETLREQRIFFDEPCFDTELEMVLKHDQHRTVPRIVVDCCDLIEQKYRRSTQPIEGIYRQCGDYNKIQTLRFSIDANDYDSLRQPDVDIHTLTGVLKLFLREIKSPLVRVNEAKTFIGQPNQWLLTDLSAKLDSLKRLIRSLPESNRDTMDYIFGHFNRITKVPLQQISAETLSISVTPSIFHTVPQGVHMQDIQQLLREGETLADCVKLMIEYQGRIFDRLRLIQTAHLLCRCVERFANHSKSEILNPKPNTPLYLPHLYTHSRFAPESVSNPDHIVYRCTADRRRLSMRNLKKTLSQPDLLFHNLF

Target ORF information:

RefSeq Version	NM_001298471.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 16F (RhoGAP16F), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298471.1

ORF Insert Sequence:

ATGCAGACGA CGACAAGTCA GCGGCCAGTA ACGCCTGGCA CTCCAGACTC GGCGCATATC 
GTAATCGGCG CCGCGGGATT CGTGGCACAC GAGAGACGCC AGCCCGGAAA GACCCTGATG 
AATTGCTTTC GCGATCGCTC CACGACGTCG TCGTCATCGT CGCAGGCCCA ACACGCCTAT 
GTGCGCGGCC ACAGCGTCAC CTCGAGCACT CTGTTTCGCC TGGAGGGCAG TGGTGGCTCC 
AATTGCACCT TGGACAAGGC GGGCAAGCAG GATACCGGGC GGCCCGAGGC GGTCGTCCTA 
CTCAGAGAGC TGAAAACCAG ACCCAACAAG CTGGCTCTGC TCAAGAGCAG CAGCAGCAAG 
GATCTGACCC GCCTGTTCCT GGACGATTCA ACCAACACAG CCGTGCTGGT GTCCAACACC 
AGTATAGATG TCTGCTCCTC AAATTCCAGC TCTGGCGGCA GTCCGGCGGT GGGCAGGTCC 
AAGTCCGGCA GGGATCGCCG CAATGGACTG GAGTGCTGCA GCAGCTGCTC CAAGCGTTGG 
CACGATCTCA AGCAGCGTAT GCACACTCTG GAGCAGGATC TGCTCGTCCA GACCACATAC 
AGCCAAGAGC TGGAGCAGAA GGTGGGCGAG ATGAGTCGCC AGTTGACGGA ACTGCTTCAG 
GAACGCGACA GGGAGAGGGA TAAGGATAAG GCGCGCTTTG CAGCCGCAGG CGGCGTTGCT 
GCGCCTGGCA AGCGGACTGC TCACGGCAGT CCCAATGTCC GACCCTCGCG CCTGGTGGCC 
TGGATGAACC TGTCCAACTG GTTCAAGAGC AGACCCAGCG TGGAAACGCT GCGGGAACAG 
CGCATCTTCT TCGACGAGCC CTGCTTCGAT ACGGAACTGG AGATGGTCCT CAAGCACGAC 
CAGCACCGCA CTGTGCCGCG CATCGTTGTG GATTGCTGCG ATCTAATCGA GCAAAAGTAC 
CGCAGATCTA CGCAGCCCAT CGAGGGCATC TATCGGCAGT GCGGCGACTA CAATAAAATC 
CAAACGCTGC GCTTCAGCAT CGACGCCAAC GACTACGATT CACTGCGTCA GCCGGACGTC 
GATATACACA CGTTGACCGG TGTGCTTAAG CTCTTCCTGC GCGAAATCAA GAGTCCGTTG 
GTCAGGGTCA ACGAGGCCAA GACCTTCATT GGACAGCCCA ATCAGTGGTT GTTAACCGAT 
CTGTCTGCGA AGTTGGACTC CCTGAAAAGA CTGATTCGTT CGCTGCCGGA GAGCAACCGG 
GACACCATGG ACTACATCTT CGGTCACTTC AATAGAATAA CCAAGGTGCC GCTGCAGCAG 
ATCAGCGCTG AAACGCTGTC CATATCGGTG ACGCCGTCGA TCTTTCACAC GGTGCCACAG 
GGCGTCCACA TGCAGGACAT CCAGCAACTT TTGCGCGAGG GCGAAACGCT GGCCGACTGC 
GTCAAGCTGA TGATCGAGTA CCAGGGGCGC ATATTCGACC GCTTACGTCT CATCCAAACC 
GCTCACCTAC TGTGCCGTTG TGTCGAACGC TTTGCTAACC ACTCGAAATC CGAAATCCTC 
AACCCGAAAC CGAACACCCC ACTGTACCTG CCACACCTCT ATACCCATTC CCGATTCGCT 
CCCGAATCTG TTTCCAATCC CGATCACATC GTCTACAGAT GCACCGCCGA TCGCCGCCGC 
CTGAGCATGC GCAACCTAAA GAAGACTCTG TCGCAGCCGG ACCTGCTCTT TCACAATCTA 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP16F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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