chas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	chas
Entrez Gene ID	32748
Full Name	chascon
Synonyms	CG12987,CG12988,CG12989,CG32556,Dmel\CG32556,EP-631
General protein information	Preferred Names chascon Names CG32556 gene product from transcript CG32556-RA CG32556-PA CG32556-PB CG32556-PC CG32556-PD CG32556-PE chas-PA chas-PB chas-PC chas-PD chas-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16B12-16C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_206773.2	NP_996496.1	chascon, isoform B
NM_001298451.1	NP_001285380.1	chascon, isoform C
NM_167574.2	NP_728092.1	chascon, isoform A
NM_001298452.1	NP_001285381.1	chascon, isoform D
NM_001298453.1	NP_001285382.1	chascon, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01927	NM_206773.2 Latest version!	Drosophila melanogaster chascon (chas), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa01927	NM_001298453.1 Latest version!	Drosophila melanogaster chascon (chas), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OFa01928	NM_001298451.1 Latest version!	Drosophila melanogaster chascon (chas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFa01928	NM_167574.2 Latest version!	Drosophila melanogaster chascon (chas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFa21520	NM_001298452.1 Latest version!	Drosophila melanogaster chascon (chas), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chas ( NM_206773.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996496.1 Drosophila melanogaster chascon (chas), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01927
Clone ID Related Accession (Same CDS sequence)	NM_206773.2 , NM_001298453.1
Accession Version	NM_206773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chascon, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206773.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCTACG CCGCCGAGAC CACCCAGATG GACAAGGACG GTCCCACCGA TCTGGAGCGA 
CCAGGTGACT GGGACAGTCT ATCCGACCGG ATGCGCAAGC TTCGCCGATC CCAGCAGCAG 
CAACAGAAGG AGGTGGCTTT GCTGGCGGAT CGCAAGGATG CGCTCACCAA GCGACCCCTG 
GACTTTGATC GCGAGGAGGA GACGCGCAAG GGCGAGACCA TGAAGTGGCT GGAGTCGCAC 
TTTGGCAGCG AGTCCACCGC CTCCAACGAT TCCCGCGACG ACGAGGCCGA TGCCGAGGTG 
GAGCCCACCA AGAAGAGTTA CTTCAATGTG ACGATCAAGT CGCAGAGCCA TGCCCAATCC 
CACCTGGCCA CCCAGCAGCA TTCGCATCCG CAGCACATCT CGCATGGCCA TCATCCACAG 
ACTTTGCCCA GAAGCGCCGA GAGGGAACGG GAGCGCGAGA GGGAACGCGA AAGGGAGCGG 
GAGAGGGAAA GGGAACGGGA GCGGGATAGG GATCGCCATC CCCTGGCCAT GACCGCATCC 
GCCTCGCAGG AGCGCAAGGC ACCGGCCAAT TCGGCTGGCG GTCGCTCCAA GTACTTCCAG 
GGCATCTCGA ACTGGTCGGA ACGCAAGGAT CCCGGTCCGC CGGTCAATCA CTTCTCGTCG 
CAGGCCTTTC GCGACGATCT GCAGGAGACC ATCCGTCGCA ATGGCCTCAA GAAGAAGGTC 
AGCGGTCCCG GCTCCAGTCA GGGATCAGCC CTGGCTCAGG AGACGGTGGT CCAGCCCAAG 
GATAGCTATG TCAGCCGCGA GGATATACTG CAGCAAAAGC GCCAGAGCCT GTCCTCCCAG 
TTCCTGGCCC GCTCGATGCG TGGCTCGCGC GATGCTCTGG ACGACCTGGA GGCCATACCG 
GGACCGGGAC CAGGTCCATC CATGATATCA CCGCGTGCCG AAGAGCCCTC GCACAAACGG 
GTGGCCTACG GTCATCGCGG CAACAATGGC ACCCACATTA ATGGCCATGC TTTGAATGGA 
GTGGCTCCCA CTCTGGTCAA TGGCCGGGGA AAGAGCTATG AGCCACCAGC TCCTCCGCCG 
GCCGCAGTTC CTCCACTAGA ATTTGGCGGC GGTGGCAGCC AGCTGGGCCA AAATCCGCGA 
CCCACTGTCC CGCAGCGACG ACGTGCCATC GAAAAGAAGC TGGGCGAGCA CAGCCACACC 
CACAGCCACA GCCACAGCCA CTCATCCCAC ACATCGCATA GCGCCCATCT GCCAGTCAAG 
TCGCAGGCTC CAATGCCGTC GCAGCTTAAC CAATTGGAGC CTCCTCCGGA CTACTCGCCG 
CCGCCCAGGA GTCGCAGTCG TTCGTCATCG CCGCAGGTCG ATTACCTGAT GACCAGGGAG 
ATGCCTGCCA GCACAATGAC GCTCAGCCGG AAGCAGCAGC AGCGCACCCG ATTTGCACCC 
ACATCCAGTT ATCCGCCGGC AACTGGAGGT ACTGGTAACC TCCGGCCACC TGGAGCCACC 
TCGGTGTCCA CTTCGAATCT ATCGGCCATG AGCGGCGGCG TCAGTACGTC CAAGTCGAGC 
CGCATGGGTC AGGTGATTGG CAATTCGCTG CGCAAGCTGG TCAGCAAAAT CCGTTCGGCC 
AGTGCGGAGC GTAAGTTTCG CATGAAATCG GCTGCCAGCA AGTCGCGCGA GCAATCGCCC 
AGTCCAGGAC AAGATCCGCG GGGCGTTGGT CCTCCGGTGA CCACCTATCA GCAGTACAAT 
GTGATCGATG GGCATATCGG TAGCCACGAT TCCGAGGATT CCAATAGCGA CAATCAGCGA 
CGCAGGGAGC GGGAGAGGGA GCGCGAAAGA GAGCGGGAAC GGGAACGGGA GCGTGATCCT 
CTCAAGCAAG AGTCCCAGTC GCGACAAAAT TTCAAGCGTG CCGAATCCGC GGATCCCCAA 
CTGCAGCACC TGGAGCAGGC CATAAGTCCC AGGCAGCGGT ACTACCTGGG CGAAGATCCC 
TACTCCAGCA CGCTCTATGG CAAGGAGAAT CACTACGAAC GACGACCACG TCAGCGTAAC 
ATGGAGCGCA CGGATGAGCG AATGGAACGA TTGGAGCGCT TAGAGCGGGA GGATGACTAC 
TACGAGTCCA GGGCACCACC ACCAGTGACG GCTGCGCACA CCTTGGGTCG CTACCAGAAG 
CATGGCCAGC GCTTCAGTGG CTCCACGCCC AATCTGCACC AGCAGCAGCA AGATTACCGC 
TCGGCTCAGA CGTTGCCACG CAAGCTGCAC GAGCAGCGCA CCCAGAGATT TGGGAATGCC 
ATGGAGAAGC AACCGGCTCC GGGCAGGGGG AGCTACCTTT CTGCTTCCGG ACAACAGCAG 
CAGTCAAGGG GGATTCCCCA TAATCAGAAT CAGCAAAATC AGCAGAATCC TAACCCAAGC 
CCGAGCATCA ACGGCAATCC AAATCCAATG CAGGCACAGC AAGGACCGGC CAAGCCGGCT 
CGCACCTACG CCAAGGTCCT GAACCGCAGC AAGAGCTTCA ATGTGCACGG CATGAACGGC 
AGCAACGATC CTAGTCCCAT TTACATCGAG AAGCTCACAA GGAACAACTA TCAGCAGCGT 
CAGGAGCCGA GCTTTGGCTA CGGCCTTGGC CAGGGACCCA ATGCCAATTA CAAGTCCAAT 
CCGCATCTAT TCTCTGGCAA GGACAACTCG CTGAAAAGCG GCCTCAAGAG TCCATCGATT 
GTGAATCTCA TCAGTCGTAG TCAGAAGGAC CTAACCAAGA TTGCTGGTAA CGATGAGGAT 
GACGTGGGCT ATCCGGAGCA GGACAAGAAG CAGTTGTATC TGCGCGGCCT GCACCAGCAG 
GCGCCAGACC TATACCGGGT GATCCATGGC GACGAAGATT ACGGGTCGCG AAAGTATCCG 
CTGCAGGCCA AGTATTCGCT GGACTCGCGT TCCCCGCCAT CCGTGGAGCT CAACAAGGAT 
ACGGCGAGCA TTGTGCGGCG CAGCAGCGAG GTGGACCACC AGCAGAGCCA TCACCACAAC 
CAGCAGCACC ACCAGCACCA CATCCAGAGC CACCAGCAGC AGAACCTAAG GCGTGGTTCG 
GGTGCCACCA TCATTGAGCT GCGCCATCGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996496.1
CDS	890..3985
Translation	MRYAAETTQMDKDGPTDLERPGDWDSLSDRMRKLRRSQQQQQKEVALLADRKDALTKRPLDFDREEETRKGETMKWLESHFGSESTASNDSRDDEADAEVEPTKKSYFNVTIKSQSHAQSHLATQQHSHPQHISHGHHPQTLPRSAERERERERERERERERERERERDRDRHPLAMTASASQERKAPANSAGGRSKYFQGISNWSERKDPGPPVNHFSSQAFRDDLQETIRRNGLKKKVSGPGSSQGSALAQETVVQPKDSYVSREDILQQKRQSLSSQFLARSMRGSRDALDDLEAIPGPGPGPSMISPRAEEPSHKRVAYGHRGNNGTHINGHALNGVAPTLVNGRGKSYEPPAPPPAAVPPLEFGGGGSQLGQNPRPTVPQRRRAIEKKLGEHSHTHSHSHSHSSHTSHSAHLPVKSQAPMPSQLNQLEPPPDYSPPPRSRSRSSSPQVDYLMTREMPASTMTLSRKQQQRTRFAPTSSYPPATGGTGNLRPPGATSVSTSNLSAMSGGVSTSKSSRMGQVIGNSLRKLVSKIRSASAERKFRMKSAASKSREQSPSPGQDPRGVGPPVTTYQQYNVIDGHIGSHDSEDSNSDNQRRRERERERERERERERERDPLKQESQSRQNFKRAESADPQLQHLEQAISPRQRYYLGEDPYSSTLYGKENHYERRPRQRNMERTDERMERLERLEREDDYYESRAPPPVTAAHTLGRYQKHGQRFSGSTPNLHQQQQDYRSAQTLPRKLHEQRTQRFGNAMEKQPAPGRGSYLSASGQQQQSRGIPHNQNQQNQQNPNPSPSINGNPNPMQAQQGPAKPARTYAKVLNRSKSFNVHGMNGSNDPSPIYIEKLTRNNYQQRQEPSFGYGLGQGPNANYKSNPHLFSGKDNSLKSGLKSPSIVNLISRSQKDLTKIAGNDEDDVGYPEQDKKQLYLRGLHQQAPDLYRVIHGDEDYGSRKYPLQAKYSLDSRSPPSVELNKDTASIVRRSSEVDHQQSHHHNQQHHQHHIQSHQQQNLRRGSGATIIELRHRK

Target ORF information:

RefSeq Version	NM_206773.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chascon (chas), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206773.2

ORF Insert Sequence:

ATGCGCTACG CCGCCGAGAC CACCCAGATG GACAAGGACG GTCCCACCGA TCTGGAGCGA 
CCAGGTGACT GGGACAGTCT ATCCGACCGG ATGCGCAAGC TTCGCCGATC CCAGCAGCAG 
CAACAGAAGG AGGTGGCTTT GCTGGCGGAT CGCAAGGATG CGCTCACCAA GCGACCCCTG 
GACTTTGATC GCGAGGAGGA GACGCGCAAG GGCGAGACCA TGAAGTGGCT GGAGTCGCAC 
TTTGGCAGCG AGTCCACCGC CTCCAACGAT TCCCGCGACG ACGAGGCCGA TGCCGAGGTG 
GAGCCCACCA AGAAGAGTTA CTTCAATGTG ACGATCAAGT CGCAGAGCCA TGCCCAATCC 
CACCTGGCCA CCCAGCAGCA TTCGCATCCG CAGCACATCT CGCATGGCCA TCATCCACAG 
ACTTTGCCCA GAAGCGCCGA GAGGGAACGG GAGCGCGAGA GGGAACGCGA AAGGGAGCGG 
GAGAGGGAAA GGGAACGGGA GCGGGATAGG GATCGCCATC CCCTGGCCAT GACCGCATCC 
GCCTCGCAGG AGCGCAAGGC ACCGGCCAAT TCGGCTGGCG GTCGCTCCAA GTACTTCCAG 
GGCATCTCGA ACTGGTCGGA ACGCAAGGAT CCCGGTCCGC CGGTCAATCA CTTCTCGTCG 
CAGGCCTTTC GCGACGATCT GCAGGAGACC ATCCGTCGCA ATGGCCTCAA GAAGAAGGTC 
AGCGGTCCCG GCTCCAGTCA GGGATCAGCC CTGGCTCAGG AGACGGTGGT CCAGCCCAAG 
GATAGCTATG TCAGCCGCGA GGATATACTG CAGCAAAAGC GCCAGAGCCT GTCCTCCCAG 
TTCCTGGCCC GCTCGATGCG TGGCTCGCGC GATGCTCTGG ACGACCTGGA GGCCATACCG 
GGACCGGGAC CAGGTCCATC CATGATATCA CCGCGTGCCG AAGAGCCCTC GCACAAACGG 
GTGGCCTACG GTCATCGCGG CAACAATGGC ACCCACATTA ATGGCCATGC TTTGAATGGA 
GTGGCTCCCA CTCTGGTCAA TGGCCGGGGA AAGAGCTATG AGCCACCAGC TCCTCCGCCG 
GCCGCAGTTC CTCCACTAGA ATTTGGCGGC GGTGGCAGCC AGCTGGGCCA AAATCCGCGA 
CCCACTGTCC CGCAGCGACG ACGTGCCATC GAAAAGAAGC TGGGCGAGCA CAGCCACACC 
CACAGCCACA GCCACAGCCA CTCATCCCAC ACATCGCATA GCGCCCATCT GCCAGTCAAG 
TCGCAGGCTC CAATGCCGTC GCAGCTTAAC CAATTGGAGC CTCCTCCGGA CTACTCGCCG 
CCGCCCAGGA GTCGCAGTCG TTCGTCATCG CCGCAGGTCG ATTACCTGAT GACCAGGGAG 
ATGCCTGCCA GCACAATGAC GCTCAGCCGG AAGCAGCAGC AGCGCACCCG ATTTGCACCC 
ACATCCAGTT ATCCGCCGGC AACTGGAGGT ACTGGTAACC TCCGGCCACC TGGAGCCACC 
TCGGTGTCCA CTTCGAATCT ATCGGCCATG AGCGGCGGCG TCAGTACGTC CAAGTCGAGC 
CGCATGGGTC AGGTGATTGG CAATTCGCTG CGCAAGCTGG TCAGCAAAAT CCGTTCGGCC 
AGTGCGGAGC GTAAGTTTCG CATGAAATCG GCTGCCAGCA AGTCGCGCGA GCAATCGCCC 
AGTCCAGGAC AAGATCCGCG GGGCGTTGGT CCTCCGGTGA CCACCTATCA GCAGTACAAT 
GTGATCGATG GGCATATCGG TAGCCACGAT TCCGAGGATT CCAATAGCGA CAATCAGCGA 
CGCAGGGAGC GGGAGAGGGA GCGCGAAAGA GAGCGGGAAC GGGAACGGGA GCGTGATCCT 
CTCAAGCAAG AGTCCCAGTC GCGACAAAAT TTCAAGCGTG CCGAATCCGC GGATCCCCAA 
CTGCAGCACC TGGAGCAGGC CATAAGTCCC AGGCAGCGGT ACTACCTGGG CGAAGATCCC 
TACTCCAGCA CGCTCTATGG CAAGGAGAAT CACTACGAAC GACGACCACG TCAGCGTAAC 
ATGGAGCGCA CGGATGAGCG AATGGAACGA TTGGAGCGCT TAGAGCGGGA GGATGACTAC 
TACGAGTCCA GGGCACCACC ACCAGTGACG GCTGCGCACA CCTTGGGTCG CTACCAGAAG 
CATGGCCAGC GCTTCAGTGG CTCCACGCCC AATCTGCACC AGCAGCAGCA AGATTACCGC 
TCGGCTCAGA CGTTGCCACG CAAGCTGCAC GAGCAGCGCA CCCAGAGATT TGGGAATGCC 
ATGGAGAAGC AACCGGCTCC GGGCAGGGGG AGCTACCTTT CTGCTTCCGG ACAACAGCAG 
CAGTCAAGGG GGATTCCCCA TAATCAGAAT CAGCAAAATC AGCAGAATCC TAACCCAAGC 
CCGAGCATCA ACGGCAATCC AAATCCAATG CAGGCACAGC AAGGACCGGC CAAGCCGGCT 
CGCACCTACG CCAAGGTCCT GAACCGCAGC AAGAGCTTCA ATGTGCACGG CATGAACGGC 
AGCAACGATC CTAGTCCCAT TTACATCGAG AAGCTCACAA GGAACAACTA TCAGCAGCGT 
CAGGAGCCGA GCTTTGGCTA CGGCCTTGGC CAGGGACCCA ATGCCAATTA CAAGTCCAAT 
CCGCATCTAT TCTCTGGCAA GGACAACTCG CTGAAAAGCG GCCTCAAGAG TCCATCGATT 
GTGAATCTCA TCAGTCGTAG TCAGAAGGAC CTAACCAAGA TTGCTGGTAA CGATGAGGAT 
GACGTGGGCT ATCCGGAGCA GGACAAGAAG CAGTTGTATC TGCGCGGCCT GCACCAGCAG 
GCGCCAGACC TATACCGGGT GATCCATGGC GACGAAGATT ACGGGTCGCG AAAGTATCCG 
CTGCAGGCCA AGTATTCGCT GGACTCGCGT TCCCCGCCAT CCGTGGAGCT CAACAAGGAT 
ACGGCGAGCA TTGTGCGGCG CAGCAGCGAG GTGGACCACC AGCAGAGCCA TCACCACAAC 
CAGCAGCACC ACCAGCACCA CATCCAGAGC CACCAGCAGC AGAACCTAAG GCGTGGTTCG 
GGTGCCACCA TCATTGAGCT GCGCCATCGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chas ( NM_001298453.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285382.1 Drosophila melanogaster chascon (chas), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa01927
Clone ID Related Accession (Same CDS sequence)	NM_206773.2 , NM_001298453.1
Accession Version	NM_001298453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chascon, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGCTACG CCGCCGAGAC CACCCAGATG GACAAGGACG GTCCCACCGA TCTGGAGCGA 
CCAGGTGACT GGGACAGTCT ATCCGACCGG ATGCGCAAGC TTCGCCGATC CCAGCAGCAG 
CAACAGAAGG AGGTGGCTTT GCTGGCGGAT CGCAAGGATG CGCTCACCAA GCGACCCCTG 
GACTTTGATC GCGAGGAGGA GACGCGCAAG GGCGAGACCA TGAAGTGGCT GGAGTCGCAC 
TTTGGCAGCG AGTCCACCGC CTCCAACGAT TCCCGCGACG ACGAGGCCGA TGCCGAGGTG 
GAGCCCACCA AGAAGAGTTA CTTCAATGTG ACGATCAAGT CGCAGAGCCA TGCCCAATCC 
CACCTGGCCA CCCAGCAGCA TTCGCATCCG CAGCACATCT CGCATGGCCA TCATCCACAG 
ACTTTGCCCA GAAGCGCCGA GAGGGAACGG GAGCGCGAGA GGGAACGCGA AAGGGAGCGG 
GAGAGGGAAA GGGAACGGGA GCGGGATAGG GATCGCCATC CCCTGGCCAT GACCGCATCC 
GCCTCGCAGG AGCGCAAGGC ACCGGCCAAT TCGGCTGGCG GTCGCTCCAA GTACTTCCAG 
GGCATCTCGA ACTGGTCGGA ACGCAAGGAT CCCGGTCCGC CGGTCAATCA CTTCTCGTCG 
CAGGCCTTTC GCGACGATCT GCAGGAGACC ATCCGTCGCA ATGGCCTCAA GAAGAAGGTC 
AGCGGTCCCG GCTCCAGTCA GGGATCAGCC CTGGCTCAGG AGACGGTGGT CCAGCCCAAG 
GATAGCTATG TCAGCCGCGA GGATATACTG CAGCAAAAGC GCCAGAGCCT GTCCTCCCAG 
TTCCTGGCCC GCTCGATGCG TGGCTCGCGC GATGCTCTGG ACGACCTGGA GGCCATACCG 
GGACCGGGAC CAGGTCCATC CATGATATCA CCGCGTGCCG AAGAGCCCTC GCACAAACGG 
GTGGCCTACG GTCATCGCGG CAACAATGGC ACCCACATTA ATGGCCATGC TTTGAATGGA 
GTGGCTCCCA CTCTGGTCAA TGGCCGGGGA AAGAGCTATG AGCCACCAGC TCCTCCGCCG 
GCCGCAGTTC CTCCACTAGA ATTTGGCGGC GGTGGCAGCC AGCTGGGCCA AAATCCGCGA 
CCCACTGTCC CGCAGCGACG ACGTGCCATC GAAAAGAAGC TGGGCGAGCA CAGCCACACC 
CACAGCCACA GCCACAGCCA CTCATCCCAC ACATCGCATA GCGCCCATCT GCCAGTCAAG 
TCGCAGGCTC CAATGCCGTC GCAGCTTAAC CAATTGGAGC CTCCTCCGGA CTACTCGCCG 
CCGCCCAGGA GTCGCAGTCG TTCGTCATCG CCGCAGGTCG ATTACCTGAT GACCAGGGAG 
ATGCCTGCCA GCACAATGAC GCTCAGCCGG AAGCAGCAGC AGCGCACCCG ATTTGCACCC 
ACATCCAGTT ATCCGCCGGC AACTGGAGGT ACTGGTAACC TCCGGCCACC TGGAGCCACC 
TCGGTGTCCA CTTCGAATCT ATCGGCCATG AGCGGCGGCG TCAGTACGTC CAAGTCGAGC 
CGCATGGGTC AGGTGATTGG CAATTCGCTG CGCAAGCTGG TCAGCAAAAT CCGTTCGGCC 
AGTGCGGAGC GTAAGTTTCG CATGAAATCG GCTGCCAGCA AGTCGCGCGA GCAATCGCCC 
AGTCCAGGAC AAGATCCGCG GGGCGTTGGT CCTCCGGTGA CCACCTATCA GCAGTACAAT 
GTGATCGATG GGCATATCGG TAGCCACGAT TCCGAGGATT CCAATAGCGA CAATCAGCGA 
CGCAGGGAGC GGGAGAGGGA GCGCGAAAGA GAGCGGGAAC GGGAACGGGA GCGTGATCCT 
CTCAAGCAAG AGTCCCAGTC GCGACAAAAT TTCAAGCGTG CCGAATCCGC GGATCCCCAA 
CTGCAGCACC TGGAGCAGGC CATAAGTCCC AGGCAGCGGT ACTACCTGGG CGAAGATCCC 
TACTCCAGCA CGCTCTATGG CAAGGAGAAT CACTACGAAC GACGACCACG TCAGCGTAAC 
ATGGAGCGCA CGGATGAGCG AATGGAACGA TTGGAGCGCT TAGAGCGGGA GGATGACTAC 
TACGAGTCCA GGGCACCACC ACCAGTGACG GCTGCGCACA CCTTGGGTCG CTACCAGAAG 
CATGGCCAGC GCTTCAGTGG CTCCACGCCC AATCTGCACC AGCAGCAGCA AGATTACCGC 
TCGGCTCAGA CGTTGCCACG CAAGCTGCAC GAGCAGCGCA CCCAGAGATT TGGGAATGCC 
ATGGAGAAGC AACCGGCTCC GGGCAGGGGG AGCTACCTTT CTGCTTCCGG ACAACAGCAG 
CAGTCAAGGG GGATTCCCCA TAATCAGAAT CAGCAAAATC AGCAGAATCC TAACCCAAGC 
CCGAGCATCA ACGGCAATCC AAATCCAATG CAGGCACAGC AAGGACCGGC CAAGCCGGCT 
CGCACCTACG CCAAGGTCCT GAACCGCAGC AAGAGCTTCA ATGTGCACGG CATGAACGGC 
AGCAACGATC CTAGTCCCAT TTACATCGAG AAGCTCACAA GGAACAACTA TCAGCAGCGT 
CAGGAGCCGA GCTTTGGCTA CGGCCTTGGC CAGGGACCCA ATGCCAATTA CAAGTCCAAT 
CCGCATCTAT TCTCTGGCAA GGACAACTCG CTGAAAAGCG GCCTCAAGAG TCCATCGATT 
GTGAATCTCA TCAGTCGTAG TCAGAAGGAC CTAACCAAGA TTGCTGGTAA CGATGAGGAT 
GACGTGGGCT ATCCGGAGCA GGACAAGAAG CAGTTGTATC TGCGCGGCCT GCACCAGCAG 
GCGCCAGACC TATACCGGGT GATCCATGGC GACGAAGATT ACGGGTCGCG AAAGTATCCG 
CTGCAGGCCA AGTATTCGCT GGACTCGCGT TCCCCGCCAT CCGTGGAGCT CAACAAGGAT 
ACGGCGAGCA TTGTGCGGCG CAGCAGCGAG GTGGACCACC AGCAGAGCCA TCACCACAAC 
CAGCAGCACC ACCAGCACCA CATCCAGAGC CACCAGCAGC AGAACCTAAG GCGTGGTTCG 
GGTGCCACCA TCATTGAGCT GCGCCATCGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285382.1
CDS	1427..4522
Translation	MRYAAETTQMDKDGPTDLERPGDWDSLSDRMRKLRRSQQQQQKEVALLADRKDALTKRPLDFDREEETRKGETMKWLESHFGSESTASNDSRDDEADAEVEPTKKSYFNVTIKSQSHAQSHLATQQHSHPQHISHGHHPQTLPRSAERERERERERERERERERERERDRDRHPLAMTASASQERKAPANSAGGRSKYFQGISNWSERKDPGPPVNHFSSQAFRDDLQETIRRNGLKKKVSGPGSSQGSALAQETVVQPKDSYVSREDILQQKRQSLSSQFLARSMRGSRDALDDLEAIPGPGPGPSMISPRAEEPSHKRVAYGHRGNNGTHINGHALNGVAPTLVNGRGKSYEPPAPPPAAVPPLEFGGGGSQLGQNPRPTVPQRRRAIEKKLGEHSHTHSHSHSHSSHTSHSAHLPVKSQAPMPSQLNQLEPPPDYSPPPRSRSRSSSPQVDYLMTREMPASTMTLSRKQQQRTRFAPTSSYPPATGGTGNLRPPGATSVSTSNLSAMSGGVSTSKSSRMGQVIGNSLRKLVSKIRSASAERKFRMKSAASKSREQSPSPGQDPRGVGPPVTTYQQYNVIDGHIGSHDSEDSNSDNQRRRERERERERERERERERDPLKQESQSRQNFKRAESADPQLQHLEQAISPRQRYYLGEDPYSSTLYGKENHYERRPRQRNMERTDERMERLERLEREDDYYESRAPPPVTAAHTLGRYQKHGQRFSGSTPNLHQQQQDYRSAQTLPRKLHEQRTQRFGNAMEKQPAPGRGSYLSASGQQQQSRGIPHNQNQQNQQNPNPSPSINGNPNPMQAQQGPAKPARTYAKVLNRSKSFNVHGMNGSNDPSPIYIEKLTRNNYQQRQEPSFGYGLGQGPNANYKSNPHLFSGKDNSLKSGLKSPSIVNLISRSQKDLTKIAGNDEDDVGYPEQDKKQLYLRGLHQQAPDLYRVIHGDEDYGSRKYPLQAKYSLDSRSPPSVELNKDTASIVRRSSEVDHQQSHHHNQQHHQHHIQSHQQQNLRRGSGATIIELRHRK

Target ORF information:

RefSeq Version	NM_001298453.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chascon (chas), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298453.1

ORF Insert Sequence:

ATGCGCTACG CCGCCGAGAC CACCCAGATG GACAAGGACG GTCCCACCGA TCTGGAGCGA 
CCAGGTGACT GGGACAGTCT ATCCGACCGG ATGCGCAAGC TTCGCCGATC CCAGCAGCAG 
CAACAGAAGG AGGTGGCTTT GCTGGCGGAT CGCAAGGATG CGCTCACCAA GCGACCCCTG 
GACTTTGATC GCGAGGAGGA GACGCGCAAG GGCGAGACCA TGAAGTGGCT GGAGTCGCAC 
TTTGGCAGCG AGTCCACCGC CTCCAACGAT TCCCGCGACG ACGAGGCCGA TGCCGAGGTG 
GAGCCCACCA AGAAGAGTTA CTTCAATGTG ACGATCAAGT CGCAGAGCCA TGCCCAATCC 
CACCTGGCCA CCCAGCAGCA TTCGCATCCG CAGCACATCT CGCATGGCCA TCATCCACAG 
ACTTTGCCCA GAAGCGCCGA GAGGGAACGG GAGCGCGAGA GGGAACGCGA AAGGGAGCGG 
GAGAGGGAAA GGGAACGGGA GCGGGATAGG GATCGCCATC CCCTGGCCAT GACCGCATCC 
GCCTCGCAGG AGCGCAAGGC ACCGGCCAAT TCGGCTGGCG GTCGCTCCAA GTACTTCCAG 
GGCATCTCGA ACTGGTCGGA ACGCAAGGAT CCCGGTCCGC CGGTCAATCA CTTCTCGTCG 
CAGGCCTTTC GCGACGATCT GCAGGAGACC ATCCGTCGCA ATGGCCTCAA GAAGAAGGTC 
AGCGGTCCCG GCTCCAGTCA GGGATCAGCC CTGGCTCAGG AGACGGTGGT CCAGCCCAAG 
GATAGCTATG TCAGCCGCGA GGATATACTG CAGCAAAAGC GCCAGAGCCT GTCCTCCCAG 
TTCCTGGCCC GCTCGATGCG TGGCTCGCGC GATGCTCTGG ACGACCTGGA GGCCATACCG 
GGACCGGGAC CAGGTCCATC CATGATATCA CCGCGTGCCG AAGAGCCCTC GCACAAACGG 
GTGGCCTACG GTCATCGCGG CAACAATGGC ACCCACATTA ATGGCCATGC TTTGAATGGA 
GTGGCTCCCA CTCTGGTCAA TGGCCGGGGA AAGAGCTATG AGCCACCAGC TCCTCCGCCG 
GCCGCAGTTC CTCCACTAGA ATTTGGCGGC GGTGGCAGCC AGCTGGGCCA AAATCCGCGA 
CCCACTGTCC CGCAGCGACG ACGTGCCATC GAAAAGAAGC TGGGCGAGCA CAGCCACACC 
CACAGCCACA GCCACAGCCA CTCATCCCAC ACATCGCATA GCGCCCATCT GCCAGTCAAG 
TCGCAGGCTC CAATGCCGTC GCAGCTTAAC CAATTGGAGC CTCCTCCGGA CTACTCGCCG 
CCGCCCAGGA GTCGCAGTCG TTCGTCATCG CCGCAGGTCG ATTACCTGAT GACCAGGGAG 
ATGCCTGCCA GCACAATGAC GCTCAGCCGG AAGCAGCAGC AGCGCACCCG ATTTGCACCC 
ACATCCAGTT ATCCGCCGGC AACTGGAGGT ACTGGTAACC TCCGGCCACC TGGAGCCACC 
TCGGTGTCCA CTTCGAATCT ATCGGCCATG AGCGGCGGCG TCAGTACGTC CAAGTCGAGC 
CGCATGGGTC AGGTGATTGG CAATTCGCTG CGCAAGCTGG TCAGCAAAAT CCGTTCGGCC 
AGTGCGGAGC GTAAGTTTCG CATGAAATCG GCTGCCAGCA AGTCGCGCGA GCAATCGCCC 
AGTCCAGGAC AAGATCCGCG GGGCGTTGGT CCTCCGGTGA CCACCTATCA GCAGTACAAT 
GTGATCGATG GGCATATCGG TAGCCACGAT TCCGAGGATT CCAATAGCGA CAATCAGCGA 
CGCAGGGAGC GGGAGAGGGA GCGCGAAAGA GAGCGGGAAC GGGAACGGGA GCGTGATCCT 
CTCAAGCAAG AGTCCCAGTC GCGACAAAAT TTCAAGCGTG CCGAATCCGC GGATCCCCAA 
CTGCAGCACC TGGAGCAGGC CATAAGTCCC AGGCAGCGGT ACTACCTGGG CGAAGATCCC 
TACTCCAGCA CGCTCTATGG CAAGGAGAAT CACTACGAAC GACGACCACG TCAGCGTAAC 
ATGGAGCGCA CGGATGAGCG AATGGAACGA TTGGAGCGCT TAGAGCGGGA GGATGACTAC 
TACGAGTCCA GGGCACCACC ACCAGTGACG GCTGCGCACA CCTTGGGTCG CTACCAGAAG 
CATGGCCAGC GCTTCAGTGG CTCCACGCCC AATCTGCACC AGCAGCAGCA AGATTACCGC 
TCGGCTCAGA CGTTGCCACG CAAGCTGCAC GAGCAGCGCA CCCAGAGATT TGGGAATGCC 
ATGGAGAAGC AACCGGCTCC GGGCAGGGGG AGCTACCTTT CTGCTTCCGG ACAACAGCAG 
CAGTCAAGGG GGATTCCCCA TAATCAGAAT CAGCAAAATC AGCAGAATCC TAACCCAAGC 
CCGAGCATCA ACGGCAATCC AAATCCAATG CAGGCACAGC AAGGACCGGC CAAGCCGGCT 
CGCACCTACG CCAAGGTCCT GAACCGCAGC AAGAGCTTCA ATGTGCACGG CATGAACGGC 
AGCAACGATC CTAGTCCCAT TTACATCGAG AAGCTCACAA GGAACAACTA TCAGCAGCGT 
CAGGAGCCGA GCTTTGGCTA CGGCCTTGGC CAGGGACCCA ATGCCAATTA CAAGTCCAAT 
CCGCATCTAT TCTCTGGCAA GGACAACTCG CTGAAAAGCG GCCTCAAGAG TCCATCGATT 
GTGAATCTCA TCAGTCGTAG TCAGAAGGAC CTAACCAAGA TTGCTGGTAA CGATGAGGAT 
GACGTGGGCT ATCCGGAGCA GGACAAGAAG CAGTTGTATC TGCGCGGCCT GCACCAGCAG 
GCGCCAGACC TATACCGGGT GATCCATGGC GACGAAGATT ACGGGTCGCG AAAGTATCCG 
CTGCAGGCCA AGTATTCGCT GGACTCGCGT TCCCCGCCAT CCGTGGAGCT CAACAAGGAT 
ACGGCGAGCA TTGTGCGGCG CAGCAGCGAG GTGGACCACC AGCAGAGCCA TCACCACAAC 
CAGCAGCACC ACCAGCACCA CATCCAGAGC CACCAGCAGC AGAACCTAAG GCGTGGTTCG 
GGTGCCACCA TCATTGAGCT GCGCCATCGC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chas ( NM_001298451.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285380.1 Drosophila melanogaster chascon (chas), transcript variant C, mRNA.

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CloneID	OFa01928
Clone ID Related Accession (Same CDS sequence)	NM_001298451.1 , NM_167574.2
Accession Version	NM_001298451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chascon, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTAA GCAAGACCAT CAAATCAAAA GCTGGCGCCA AAGACGAACA AAACGAACTG 
GTCGAATTCG AACCCAAAGA CGACGAGGAA AGCAACGAGG TCACGGCAAG CAATTGCGAT 
ACAGAAGCTA TTACCAAAGA AGAGGAGTGG ATAACGAAGC GCAAGACAGA ACTAACAACT 
ACCAGACAGA TCGAGACGAG GGTGAAGCGA CAGGTGAAGC TGCAGGATGG CAAAGTGATC 
GAGGATTCGG GGCCGATTGT CTCCACCAAC ACCACCGAGG ACACCGACAA ACAGGAAACG 
GAAACCACTG AGAAACGCGA CCTTGACCTG CCGGATGACC TCGATGGCAA TGCCCAGCTG 
ATCGACGTGG CCGCCCTGCA GGCGGGCGCC ATAAATGAAA CGCAGTTGGC CCAAATTCAA 
GAGGTCTTCG GACCGGCCGA TGGAGCCGGA AAGCTGGTCA AGCGCATGGT TCCGCGTCCG 
GTGGATGGAC TGGTGCGTCA AACGGACGAC AAGAGGGTCA TATCGCACGA GGAGATCAAG 
GACTACCACG AAACGGAGGA CGTACAGCAT CACGGCGACT TTACAGATCA GACCTACGTG 
AACGCAGTTC GCGACGGAGT CGAGAACCTA GAGGCCGTGC TCTGTTCGCC GGAGAGCCAG 
CGGCAGTTGG TGCCGCTCGG ACCCAAGGTG GTTGCCAACC GGAGCAAGTC GCGAAAGACC 
ATCGATTCGG AGGACACACA GAAGCGCTGC CTGGCCCAGG AGGACGGCAC CCTGGTCACC 
GAGAGTCGAC GGACCACCGA GCACGAGTTG ATCGTCGACG ACGAACTGCC CGACCCGCCT 
GCCAACGGCG CCTCCGATGA TCTTGGCCTG CAGCTGCAGG TGGGCGAGCA GGTGACAACG 
ACGGCGGCCA ACCAGCGGTT CTTCCGCCAA CGGGACGAGC AGCATGTGGA CGTGCTAGCC 
AACGGTCGGC TCCAATCCAC CGAGATGCGC TACGCCGCCG AGACCACCCA GATGGACAAG 
GACGGTCCCA CCGATCTGGA GCGACCAGGT GACTGGGACA GTCTATCCGA CCGGATGCGC 
AAGCTTCGCC GATCCCAGCA GCAGCAACAG AAGGAGGTGG CTTTGCTGGC GGATCGCAAG 
GATGCGCTCA CCAAGCGACC CCTGGACTTT GATCGCGAGG AGGAGACGCG CAAGGGCGAG 
ACCATGAAGT GGCTGGAGTC GCACTTTGGC AGCGAGTCCA CCGCCTCCAA CGATTCCCGC 
GACGACGAGG CCGATGCCGA GGTGGAGCCC ACCAAGAAGA GTTACTTCAA TGTGACGATC 
AAGTCGCAGA GCCATGCCCA ATCCCACCTG GCCACCCAGC AGCATTCGCA TCCGCAGCAC 
ATCTCGCATG GCCATCATCC ACAGACTTTG CCCAGAAGCG CCGAGAGGGA ACGGGAGCGC 
GAGAGGGAAC GCGAAAGGGA GCGGGAGAGG GAAAGGGAAC GGGAGCGGGA TAGGGATCGC 
CATCCCCTGG CCATGACCGC ATCCGCCTCG CAGGAGCGCA AGGCACCGGC CAATTCGGCT 
GGCGGTCGCT CCAAGTACTT CCAGGGCATC TCGAACTGGT CGGAACGCAA GGATCCCGGT 
CCGCCGGTCA ATCACTTCTC GTCGCAGGCC TTTCGCGACG ATCTGCAGGA GACCATCCGT 
CGCAATGGCC TCAAGAAGAA GGTCAGCGGT CCCGGCTCCA GTCAGGGATC AGCCCTGGCT 
CAGGAGACGG TGGTCCAGCC CAAGGATAGC TATGTCAGCC GCGAGGATAT ACTGCAGCAA 
AAGCGCCAGA GCCTGTCCTC CCAGTTCCTG GCCCGCTCGA TGCGTGGCTC GCGCGATGCT 
CTGGACGACC TGGAGGCCAT ACCGGGACCG GGACCAGGTC CATCCATGAT ATCACCGCGT 
GCCGAAGAGC CCTCGCACAA ACGGGTGGCC TACGGTCATC GCGGCAACAA TGGCACCCAC 
ATTAATGGCC ATGCTTTGAA TGGAGTGGCT CCCACTCTGG TCAATGGCCG GGGAAAGAGC 
TATGAGCCAC CAGCTCCTCC GCCGGCCGCA GTTCCTCCAC TAGAATTTGG CGGCGGTGGC 
AGCCAGCTGG GCCAAAATCC GCGACCCACT GTCCCGCAGC GACGACGTGC CATCGAAAAG 
AAGCTGGGCG AGCACAGCCA CACCCACAGC CACAGCCACA GCCACTCATC CCACACATCG 
CATAGCGCCC ATCTGCCAGT CAAGTCGCAG GCTCCAATGC CGTCGCAGCT TAACCAATTG 
GAGCCTCCTC CGGACTACTC GCCGCCGCCC AGGAGTCGCA GTCGTTCGTC ATCGCCGCAG 
GTCGATTACC TGATGACCAG GGAGATGCCT GCCAGCACAA TGACGCTCAG CCGGAAGCAG 
CAGCAGCGCA CCCGATTTGC ACCCACATCC AGTTATCCGC CGGCAACTGG AGGTACTGGT 
AACCTCCGGC CACCTGGAGC CACCTCGGTG TCCACTTCGA ATCTATCGGC CATGAGCGGC 
GGCGTCAGTA CGTCCAAGTC GAGCCGCATG GGTCAGGTGA TTGGCAATTC GCTGCGCAAG 
CTGGTCAGCA AAATCCGTTC GGCCAGTGCG GAGCGTAAGT TTCGCATGAA ATCGGCTGCC 
AGCAAGTCGC GCGAGCAATC GCCCAGTCCA GGACAAGATC CGCGGGGCGT TGGTCCTCCG 
GTGACCACCT ATCAGCAGTA CAATGTGATC GATGGGCATA TCGGTAGCCA CGATTCCGAG 
GATTCCAATA GCGACAATCA GCGACGCAGG GAGCGGGAGA GGGAGCGCGA AAGAGAGCGG 
GAACGGGAAC GGGAGCGTGA TCCTCTCAAG CAAGAGTCCC AGTCGCGACA AAATTTCAAG 
CGTGCCGAAT CCGCGGATCC CCAACTGCAG CACCTGGAGC AGGCCATAAG TCCCAGGCAG 
CGGTACTACC TGGGCGAAGA TCCCTACTCC AGCACGCTCT ATGGCAAGGA GAATCACTAC 
GAACGACGAC CACGTCAGCG TAACATGGAG CGCACGGATG AGCGAATGGA ACGATTGGAG 
CGCTTAGAGC GGGAGGATGA CTACTACGAG TCCAGGGCAC CACCACCAGT GACGGCTGCG 
CACACCTTGG GTCGCTACCA GAAGCATGGC CAGCGCTTCA GTGGCTCCAC GCCCAATCTG 
CACCAGCAGC AGCAAGATTA CCGCTCGGCT CAGACGTTGC CACGCAAGCT GCACGAGCAG 
CGCACCCAGA GATTTGGGAA TGCCATGGAG AAGCAACCGG CTCCGGGCAG GGGGAGCTAC 
CTTTCTGCTT CCGGACAACA GCAGCAGTCA AGGGGGATTC CCCATAATCA GAATCAGCAA 
AATCAGCAGA ATCCTAACCC AAGCCCGAGC ATCAACGGCA ATCCAAATCC AATGCAGGCA 
CAGCAAGGAC CGGCCAAGCC GGCTCGCACC TACGCCAAGG TCCTGAACCG CAGCAAGAGC 
TTCAATGTGC ACGGCATGAA CGGCAGCAAC GATCCTAGTC CCATTTACAT CGAGAAGCTC 
ACAAGGAACA ACTATCAGCA GCGTCAGGAG CCGAGCTTTG GCTACGGCCT TGGCCAGGGA 
CCCAATGCCA ATTACAAGTC CAATCCGCAT CTATTCTCTG GCAAGGACAA CTCGCTGAAA 
AGCGGCCTCA AGAGTCCATC GATTGTGAAT CTCATCAGTC GTAGTCAGAA GGACCTAACC 
AAGATTGCTG GTAACGATGA GGATGACGTG GGCTATCCGG AGCAGGACAA GAAGCAGTTG 
TATCTGCGCG GCCTGCACCA GCAGGCGCCA GACCTATACC GGGTGATCCA TGGCGACGAA 
GATTACGGGT CGCGAAAGTA TCCGCTGCAG GCCAAGTATT CGCTGGACTC GCGTTCCCCG 
CCATCCGTGG AGCTCAACAA GGATACGGCG AGCATTGTGC GGCGCAGCAG CGAGGTGGAC 
CACCAGCAGA GCCATCACCA CAACCAGCAG CACCACCAGC ACCACATCCA GAGCCACCAG 
CAGCAGAACC TAAGGCGTGG TTCGGGTGCC ACCATCATTG AGCTGCGCCA TCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285380.1
CDS	399..4478
Translation	MALSKTIKSKAGAKDEQNELVEFEPKDDEESNEVTASNCDTEAITKEEEWITKRKTELTTTRQIETRVKRQVKLQDGKVIEDSGPIVSTNTTEDTDKQETETTEKRDLDLPDDLDGNAQLIDVAALQAGAINETQLAQIQEVFGPADGAGKLVKRMVPRPVDGLVRQTDDKRVISHEEIKDYHETEDVQHHGDFTDQTYVNAVRDGVENLEAVLCSPESQRQLVPLGPKVVANRSKSRKTIDSEDTQKRCLAQEDGTLVTESRRTTEHELIVDDELPDPPANGASDDLGLQLQVGEQVTTTAANQRFFRQRDEQHVDVLANGRLQSTEMRYAAETTQMDKDGPTDLERPGDWDSLSDRMRKLRRSQQQQQKEVALLADRKDALTKRPLDFDREEETRKGETMKWLESHFGSESTASNDSRDDEADAEVEPTKKSYFNVTIKSQSHAQSHLATQQHSHPQHISHGHHPQTLPRSAERERERERERERERERERERERDRDRHPLAMTASASQERKAPANSAGGRSKYFQGISNWSERKDPGPPVNHFSSQAFRDDLQETIRRNGLKKKVSGPGSSQGSALAQETVVQPKDSYVSREDILQQKRQSLSSQFLARSMRGSRDALDDLEAIPGPGPGPSMISPRAEEPSHKRVAYGHRGNNGTHINGHALNGVAPTLVNGRGKSYEPPAPPPAAVPPLEFGGGGSQLGQNPRPTVPQRRRAIEKKLGEHSHTHSHSHSHSSHTSHSAHLPVKSQAPMPSQLNQLEPPPDYSPPPRSRSRSSSPQVDYLMTREMPASTMTLSRKQQQRTRFAPTSSYPPATGGTGNLRPPGATSVSTSNLSAMSGGVSTSKSSRMGQVIGNSLRKLVSKIRSASAERKFRMKSAASKSREQSPSPGQDPRGVGPPVTTYQQYNVIDGHIGSHDSEDSNSDNQRRRERERERERERERERERDPLKQESQSRQNFKRAESADPQLQHLEQAISPRQRYYLGEDPYSSTLYGKENHYERRPRQRNMERTDERMERLERLEREDDYYESRAPPPVTAAHTLGRYQKHGQRFSGSTPNLHQQQQDYRSAQTLPRKLHEQRTQRFGNAMEKQPAPGRGSYLSASGQQQQSRGIPHNQNQQNQQNPNPSPSINGNPNPMQAQQGPAKPARTYAKVLNRSKSFNVHGMNGSNDPSPIYIEKLTRNNYQQRQEPSFGYGLGQGPNANYKSNPHLFSGKDNSLKSGLKSPSIVNLISRSQKDLTKIAGNDEDDVGYPEQDKKQLYLRGLHQQAPDLYRVIHGDEDYGSRKYPLQAKYSLDSRSPPSVELNKDTASIVRRSSEVDHQQSHHHNQQHHQHHIQSHQQQNLRRGSGATIIELRHRK

Target ORF information:

RefSeq Version	NM_001298451.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chascon (chas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298451.1

ORF Insert Sequence:

ATGGCTTTAA GCAAGACCAT CAAATCAAAA GCTGGCGCCA AAGACGAACA AAACGAACTG 
GTCGAATTCG AACCCAAAGA CGACGAGGAA AGCAACGAGG TCACGGCAAG CAATTGCGAT 
ACAGAAGCTA TTACCAAAGA AGAGGAGTGG ATAACGAAGC GCAAGACAGA ACTAACAACT 
ACCAGACAGA TCGAGACGAG GGTGAAGCGA CAGGTGAAGC TGCAGGATGG CAAAGTGATC 
GAGGATTCGG GGCCGATTGT CTCCACCAAC ACCACCGAGG ACACCGACAA ACAGGAAACG 
GAAACCACTG AGAAACGCGA CCTTGACCTG CCGGATGACC TCGATGGCAA TGCCCAGCTG 
ATCGACGTGG CCGCCCTGCA GGCGGGCGCC ATAAATGAAA CGCAGTTGGC CCAAATTCAA 
GAGGTCTTCG GACCGGCCGA TGGAGCCGGA AAGCTGGTCA AGCGCATGGT TCCGCGTCCG 
GTGGATGGAC TGGTGCGTCA AACGGACGAC AAGAGGGTCA TATCGCACGA GGAGATCAAG 
GACTACCACG AAACGGAGGA CGTACAGCAT CACGGCGACT TTACAGATCA GACCTACGTG 
AACGCAGTTC GCGACGGAGT CGAGAACCTA GAGGCCGTGC TCTGTTCGCC GGAGAGCCAG 
CGGCAGTTGG TGCCGCTCGG ACCCAAGGTG GTTGCCAACC GGAGCAAGTC GCGAAAGACC 
ATCGATTCGG AGGACACACA GAAGCGCTGC CTGGCCCAGG AGGACGGCAC CCTGGTCACC 
GAGAGTCGAC GGACCACCGA GCACGAGTTG ATCGTCGACG ACGAACTGCC CGACCCGCCT 
GCCAACGGCG CCTCCGATGA TCTTGGCCTG CAGCTGCAGG TGGGCGAGCA GGTGACAACG 
ACGGCGGCCA ACCAGCGGTT CTTCCGCCAA CGGGACGAGC AGCATGTGGA CGTGCTAGCC 
AACGGTCGGC TCCAATCCAC CGAGATGCGC TACGCCGCCG AGACCACCCA GATGGACAAG 
GACGGTCCCA CCGATCTGGA GCGACCAGGT GACTGGGACA GTCTATCCGA CCGGATGCGC 
AAGCTTCGCC GATCCCAGCA GCAGCAACAG AAGGAGGTGG CTTTGCTGGC GGATCGCAAG 
GATGCGCTCA CCAAGCGACC CCTGGACTTT GATCGCGAGG AGGAGACGCG CAAGGGCGAG 
ACCATGAAGT GGCTGGAGTC GCACTTTGGC AGCGAGTCCA CCGCCTCCAA CGATTCCCGC 
GACGACGAGG CCGATGCCGA GGTGGAGCCC ACCAAGAAGA GTTACTTCAA TGTGACGATC 
AAGTCGCAGA GCCATGCCCA ATCCCACCTG GCCACCCAGC AGCATTCGCA TCCGCAGCAC 
ATCTCGCATG GCCATCATCC ACAGACTTTG CCCAGAAGCG CCGAGAGGGA ACGGGAGCGC 
GAGAGGGAAC GCGAAAGGGA GCGGGAGAGG GAAAGGGAAC GGGAGCGGGA TAGGGATCGC 
CATCCCCTGG CCATGACCGC ATCCGCCTCG CAGGAGCGCA AGGCACCGGC CAATTCGGCT 
GGCGGTCGCT CCAAGTACTT CCAGGGCATC TCGAACTGGT CGGAACGCAA GGATCCCGGT 
CCGCCGGTCA ATCACTTCTC GTCGCAGGCC TTTCGCGACG ATCTGCAGGA GACCATCCGT 
CGCAATGGCC TCAAGAAGAA GGTCAGCGGT CCCGGCTCCA GTCAGGGATC AGCCCTGGCT 
CAGGAGACGG TGGTCCAGCC CAAGGATAGC TATGTCAGCC GCGAGGATAT ACTGCAGCAA 
AAGCGCCAGA GCCTGTCCTC CCAGTTCCTG GCCCGCTCGA TGCGTGGCTC GCGCGATGCT 
CTGGACGACC TGGAGGCCAT ACCGGGACCG GGACCAGGTC CATCCATGAT ATCACCGCGT 
GCCGAAGAGC CCTCGCACAA ACGGGTGGCC TACGGTCATC GCGGCAACAA TGGCACCCAC 
ATTAATGGCC ATGCTTTGAA TGGAGTGGCT CCCACTCTGG TCAATGGCCG GGGAAAGAGC 
TATGAGCCAC CAGCTCCTCC GCCGGCCGCA GTTCCTCCAC TAGAATTTGG CGGCGGTGGC 
AGCCAGCTGG GCCAAAATCC GCGACCCACT GTCCCGCAGC GACGACGTGC CATCGAAAAG 
AAGCTGGGCG AGCACAGCCA CACCCACAGC CACAGCCACA GCCACTCATC CCACACATCG 
CATAGCGCCC ATCTGCCAGT CAAGTCGCAG GCTCCAATGC CGTCGCAGCT TAACCAATTG 
GAGCCTCCTC CGGACTACTC GCCGCCGCCC AGGAGTCGCA GTCGTTCGTC ATCGCCGCAG 
GTCGATTACC TGATGACCAG GGAGATGCCT GCCAGCACAA TGACGCTCAG CCGGAAGCAG 
CAGCAGCGCA CCCGATTTGC ACCCACATCC AGTTATCCGC CGGCAACTGG AGGTACTGGT 
AACCTCCGGC CACCTGGAGC CACCTCGGTG TCCACTTCGA ATCTATCGGC CATGAGCGGC 
GGCGTCAGTA CGTCCAAGTC GAGCCGCATG GGTCAGGTGA TTGGCAATTC GCTGCGCAAG 
CTGGTCAGCA AAATCCGTTC GGCCAGTGCG GAGCGTAAGT TTCGCATGAA ATCGGCTGCC 
AGCAAGTCGC GCGAGCAATC GCCCAGTCCA GGACAAGATC CGCGGGGCGT TGGTCCTCCG 
GTGACCACCT ATCAGCAGTA CAATGTGATC GATGGGCATA TCGGTAGCCA CGATTCCGAG 
GATTCCAATA GCGACAATCA GCGACGCAGG GAGCGGGAGA GGGAGCGCGA AAGAGAGCGG 
GAACGGGAAC GGGAGCGTGA TCCTCTCAAG CAAGAGTCCC AGTCGCGACA AAATTTCAAG 
CGTGCCGAAT CCGCGGATCC CCAACTGCAG CACCTGGAGC AGGCCATAAG TCCCAGGCAG 
CGGTACTACC TGGGCGAAGA TCCCTACTCC AGCACGCTCT ATGGCAAGGA GAATCACTAC 
GAACGACGAC CACGTCAGCG TAACATGGAG CGCACGGATG AGCGAATGGA ACGATTGGAG 
CGCTTAGAGC GGGAGGATGA CTACTACGAG TCCAGGGCAC CACCACCAGT GACGGCTGCG 
CACACCTTGG GTCGCTACCA GAAGCATGGC CAGCGCTTCA GTGGCTCCAC GCCCAATCTG 
CACCAGCAGC AGCAAGATTA CCGCTCGGCT CAGACGTTGC CACGCAAGCT GCACGAGCAG 
CGCACCCAGA GATTTGGGAA TGCCATGGAG AAGCAACCGG CTCCGGGCAG GGGGAGCTAC 
CTTTCTGCTT CCGGACAACA GCAGCAGTCA AGGGGGATTC CCCATAATCA GAATCAGCAA 
AATCAGCAGA ATCCTAACCC AAGCCCGAGC ATCAACGGCA ATCCAAATCC AATGCAGGCA 
CAGCAAGGAC CGGCCAAGCC GGCTCGCACC TACGCCAAGG TCCTGAACCG CAGCAAGAGC 
TTCAATGTGC ACGGCATGAA CGGCAGCAAC GATCCTAGTC CCATTTACAT CGAGAAGCTC 
ACAAGGAACA ACTATCAGCA GCGTCAGGAG CCGAGCTTTG GCTACGGCCT TGGCCAGGGA 
CCCAATGCCA ATTACAAGTC CAATCCGCAT CTATTCTCTG GCAAGGACAA CTCGCTGAAA 
AGCGGCCTCA AGAGTCCATC GATTGTGAAT CTCATCAGTC GTAGTCAGAA GGACCTAACC 
AAGATTGCTG GTAACGATGA GGATGACGTG GGCTATCCGG AGCAGGACAA GAAGCAGTTG 
TATCTGCGCG GCCTGCACCA GCAGGCGCCA GACCTATACC GGGTGATCCA TGGCGACGAA 
GATTACGGGT CGCGAAAGTA TCCGCTGCAG GCCAAGTATT CGCTGGACTC GCGTTCCCCG 
CCATCCGTGG AGCTCAACAA GGATACGGCG AGCATTGTGC GGCGCAGCAG CGAGGTGGAC 
CACCAGCAGA GCCATCACCA CAACCAGCAG CACCACCAGC ACCACATCCA GAGCCACCAG 
CAGCAGAACC TAAGGCGTGG TTCGGGTGCC ACCATCATTG AGCTGCGCCA TCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chas ( NM_167574.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728092.1 Drosophila melanogaster chascon (chas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01928
Clone ID Related Accession (Same CDS sequence)	NM_001298451.1 , NM_167574.2
Accession Version	NM_167574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chascon, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167574.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTTTAA GCAAGACCAT CAAATCAAAA GCTGGCGCCA AAGACGAACA AAACGAACTG 
GTCGAATTCG AACCCAAAGA CGACGAGGAA AGCAACGAGG TCACGGCAAG CAATTGCGAT 
ACAGAAGCTA TTACCAAAGA AGAGGAGTGG ATAACGAAGC GCAAGACAGA ACTAACAACT 
ACCAGACAGA TCGAGACGAG GGTGAAGCGA CAGGTGAAGC TGCAGGATGG CAAAGTGATC 
GAGGATTCGG GGCCGATTGT CTCCACCAAC ACCACCGAGG ACACCGACAA ACAGGAAACG 
GAAACCACTG AGAAACGCGA CCTTGACCTG CCGGATGACC TCGATGGCAA TGCCCAGCTG 
ATCGACGTGG CCGCCCTGCA GGCGGGCGCC ATAAATGAAA CGCAGTTGGC CCAAATTCAA 
GAGGTCTTCG GACCGGCCGA TGGAGCCGGA AAGCTGGTCA AGCGCATGGT TCCGCGTCCG 
GTGGATGGAC TGGTGCGTCA AACGGACGAC AAGAGGGTCA TATCGCACGA GGAGATCAAG 
GACTACCACG AAACGGAGGA CGTACAGCAT CACGGCGACT TTACAGATCA GACCTACGTG 
AACGCAGTTC GCGACGGAGT CGAGAACCTA GAGGCCGTGC TCTGTTCGCC GGAGAGCCAG 
CGGCAGTTGG TGCCGCTCGG ACCCAAGGTG GTTGCCAACC GGAGCAAGTC GCGAAAGACC 
ATCGATTCGG AGGACACACA GAAGCGCTGC CTGGCCCAGG AGGACGGCAC CCTGGTCACC 
GAGAGTCGAC GGACCACCGA GCACGAGTTG ATCGTCGACG ACGAACTGCC CGACCCGCCT 
GCCAACGGCG CCTCCGATGA TCTTGGCCTG CAGCTGCAGG TGGGCGAGCA GGTGACAACG 
ACGGCGGCCA ACCAGCGGTT CTTCCGCCAA CGGGACGAGC AGCATGTGGA CGTGCTAGCC 
AACGGTCGGC TCCAATCCAC CGAGATGCGC TACGCCGCCG AGACCACCCA GATGGACAAG 
GACGGTCCCA CCGATCTGGA GCGACCAGGT GACTGGGACA GTCTATCCGA CCGGATGCGC 
AAGCTTCGCC GATCCCAGCA GCAGCAACAG AAGGAGGTGG CTTTGCTGGC GGATCGCAAG 
GATGCGCTCA CCAAGCGACC CCTGGACTTT GATCGCGAGG AGGAGACGCG CAAGGGCGAG 
ACCATGAAGT GGCTGGAGTC GCACTTTGGC AGCGAGTCCA CCGCCTCCAA CGATTCCCGC 
GACGACGAGG CCGATGCCGA GGTGGAGCCC ACCAAGAAGA GTTACTTCAA TGTGACGATC 
AAGTCGCAGA GCCATGCCCA ATCCCACCTG GCCACCCAGC AGCATTCGCA TCCGCAGCAC 
ATCTCGCATG GCCATCATCC ACAGACTTTG CCCAGAAGCG CCGAGAGGGA ACGGGAGCGC 
GAGAGGGAAC GCGAAAGGGA GCGGGAGAGG GAAAGGGAAC GGGAGCGGGA TAGGGATCGC 
CATCCCCTGG CCATGACCGC ATCCGCCTCG CAGGAGCGCA AGGCACCGGC CAATTCGGCT 
GGCGGTCGCT CCAAGTACTT CCAGGGCATC TCGAACTGGT CGGAACGCAA GGATCCCGGT 
CCGCCGGTCA ATCACTTCTC GTCGCAGGCC TTTCGCGACG ATCTGCAGGA GACCATCCGT 
CGCAATGGCC TCAAGAAGAA GGTCAGCGGT CCCGGCTCCA GTCAGGGATC AGCCCTGGCT 
CAGGAGACGG TGGTCCAGCC CAAGGATAGC TATGTCAGCC GCGAGGATAT ACTGCAGCAA 
AAGCGCCAGA GCCTGTCCTC CCAGTTCCTG GCCCGCTCGA TGCGTGGCTC GCGCGATGCT 
CTGGACGACC TGGAGGCCAT ACCGGGACCG GGACCAGGTC CATCCATGAT ATCACCGCGT 
GCCGAAGAGC CCTCGCACAA ACGGGTGGCC TACGGTCATC GCGGCAACAA TGGCACCCAC 
ATTAATGGCC ATGCTTTGAA TGGAGTGGCT CCCACTCTGG TCAATGGCCG GGGAAAGAGC 
TATGAGCCAC CAGCTCCTCC GCCGGCCGCA GTTCCTCCAC TAGAATTTGG CGGCGGTGGC 
AGCCAGCTGG GCCAAAATCC GCGACCCACT GTCCCGCAGC GACGACGTGC CATCGAAAAG 
AAGCTGGGCG AGCACAGCCA CACCCACAGC CACAGCCACA GCCACTCATC CCACACATCG 
CATAGCGCCC ATCTGCCAGT CAAGTCGCAG GCTCCAATGC CGTCGCAGCT TAACCAATTG 
GAGCCTCCTC CGGACTACTC GCCGCCGCCC AGGAGTCGCA GTCGTTCGTC ATCGCCGCAG 
GTCGATTACC TGATGACCAG GGAGATGCCT GCCAGCACAA TGACGCTCAG CCGGAAGCAG 
CAGCAGCGCA CCCGATTTGC ACCCACATCC AGTTATCCGC CGGCAACTGG AGGTACTGGT 
AACCTCCGGC CACCTGGAGC CACCTCGGTG TCCACTTCGA ATCTATCGGC CATGAGCGGC 
GGCGTCAGTA CGTCCAAGTC GAGCCGCATG GGTCAGGTGA TTGGCAATTC GCTGCGCAAG 
CTGGTCAGCA AAATCCGTTC GGCCAGTGCG GAGCGTAAGT TTCGCATGAA ATCGGCTGCC 
AGCAAGTCGC GCGAGCAATC GCCCAGTCCA GGACAAGATC CGCGGGGCGT TGGTCCTCCG 
GTGACCACCT ATCAGCAGTA CAATGTGATC GATGGGCATA TCGGTAGCCA CGATTCCGAG 
GATTCCAATA GCGACAATCA GCGACGCAGG GAGCGGGAGA GGGAGCGCGA AAGAGAGCGG 
GAACGGGAAC GGGAGCGTGA TCCTCTCAAG CAAGAGTCCC AGTCGCGACA AAATTTCAAG 
CGTGCCGAAT CCGCGGATCC CCAACTGCAG CACCTGGAGC AGGCCATAAG TCCCAGGCAG 
CGGTACTACC TGGGCGAAGA TCCCTACTCC AGCACGCTCT ATGGCAAGGA GAATCACTAC 
GAACGACGAC CACGTCAGCG TAACATGGAG CGCACGGATG AGCGAATGGA ACGATTGGAG 
CGCTTAGAGC GGGAGGATGA CTACTACGAG TCCAGGGCAC CACCACCAGT GACGGCTGCG 
CACACCTTGG GTCGCTACCA GAAGCATGGC CAGCGCTTCA GTGGCTCCAC GCCCAATCTG 
CACCAGCAGC AGCAAGATTA CCGCTCGGCT CAGACGTTGC CACGCAAGCT GCACGAGCAG 
CGCACCCAGA GATTTGGGAA TGCCATGGAG AAGCAACCGG CTCCGGGCAG GGGGAGCTAC 
CTTTCTGCTT CCGGACAACA GCAGCAGTCA AGGGGGATTC CCCATAATCA GAATCAGCAA 
AATCAGCAGA ATCCTAACCC AAGCCCGAGC ATCAACGGCA ATCCAAATCC AATGCAGGCA 
CAGCAAGGAC CGGCCAAGCC GGCTCGCACC TACGCCAAGG TCCTGAACCG CAGCAAGAGC 
TTCAATGTGC ACGGCATGAA CGGCAGCAAC GATCCTAGTC CCATTTACAT CGAGAAGCTC 
ACAAGGAACA ACTATCAGCA GCGTCAGGAG CCGAGCTTTG GCTACGGCCT TGGCCAGGGA 
CCCAATGCCA ATTACAAGTC CAATCCGCAT CTATTCTCTG GCAAGGACAA CTCGCTGAAA 
AGCGGCCTCA AGAGTCCATC GATTGTGAAT CTCATCAGTC GTAGTCAGAA GGACCTAACC 
AAGATTGCTG GTAACGATGA GGATGACGTG GGCTATCCGG AGCAGGACAA GAAGCAGTTG 
TATCTGCGCG GCCTGCACCA GCAGGCGCCA GACCTATACC GGGTGATCCA TGGCGACGAA 
GATTACGGGT CGCGAAAGTA TCCGCTGCAG GCCAAGTATT CGCTGGACTC GCGTTCCCCG 
CCATCCGTGG AGCTCAACAA GGATACGGCG AGCATTGTGC GGCGCAGCAG CGAGGTGGAC 
CACCAGCAGA GCCATCACCA CAACCAGCAG CACCACCAGC ACCACATCCA GAGCCACCAG 
CAGCAGAACC TAAGGCGTGG TTCGGGTGCC ACCATCATTG AGCTGCGCCA TCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728092.1
CDS	448..4527
Translation	MALSKTIKSKAGAKDEQNELVEFEPKDDEESNEVTASNCDTEAITKEEEWITKRKTELTTTRQIETRVKRQVKLQDGKVIEDSGPIVSTNTTEDTDKQETETTEKRDLDLPDDLDGNAQLIDVAALQAGAINETQLAQIQEVFGPADGAGKLVKRMVPRPVDGLVRQTDDKRVISHEEIKDYHETEDVQHHGDFTDQTYVNAVRDGVENLEAVLCSPESQRQLVPLGPKVVANRSKSRKTIDSEDTQKRCLAQEDGTLVTESRRTTEHELIVDDELPDPPANGASDDLGLQLQVGEQVTTTAANQRFFRQRDEQHVDVLANGRLQSTEMRYAAETTQMDKDGPTDLERPGDWDSLSDRMRKLRRSQQQQQKEVALLADRKDALTKRPLDFDREEETRKGETMKWLESHFGSESTASNDSRDDEADAEVEPTKKSYFNVTIKSQSHAQSHLATQQHSHPQHISHGHHPQTLPRSAERERERERERERERERERERERDRDRHPLAMTASASQERKAPANSAGGRSKYFQGISNWSERKDPGPPVNHFSSQAFRDDLQETIRRNGLKKKVSGPGSSQGSALAQETVVQPKDSYVSREDILQQKRQSLSSQFLARSMRGSRDALDDLEAIPGPGPGPSMISPRAEEPSHKRVAYGHRGNNGTHINGHALNGVAPTLVNGRGKSYEPPAPPPAAVPPLEFGGGGSQLGQNPRPTVPQRRRAIEKKLGEHSHTHSHSHSHSSHTSHSAHLPVKSQAPMPSQLNQLEPPPDYSPPPRSRSRSSSPQVDYLMTREMPASTMTLSRKQQQRTRFAPTSSYPPATGGTGNLRPPGATSVSTSNLSAMSGGVSTSKSSRMGQVIGNSLRKLVSKIRSASAERKFRMKSAASKSREQSPSPGQDPRGVGPPVTTYQQYNVIDGHIGSHDSEDSNSDNQRRRERERERERERERERERDPLKQESQSRQNFKRAESADPQLQHLEQAISPRQRYYLGEDPYSSTLYGKENHYERRPRQRNMERTDERMERLERLEREDDYYESRAPPPVTAAHTLGRYQKHGQRFSGSTPNLHQQQQDYRSAQTLPRKLHEQRTQRFGNAMEKQPAPGRGSYLSASGQQQQSRGIPHNQNQQNQQNPNPSPSINGNPNPMQAQQGPAKPARTYAKVLNRSKSFNVHGMNGSNDPSPIYIEKLTRNNYQQRQEPSFGYGLGQGPNANYKSNPHLFSGKDNSLKSGLKSPSIVNLISRSQKDLTKIAGNDEDDVGYPEQDKKQLYLRGLHQQAPDLYRVIHGDEDYGSRKYPLQAKYSLDSRSPPSVELNKDTASIVRRSSEVDHQQSHHHNQQHHQHHIQSHQQQNLRRGSGATIIELRHRK

Target ORF information:

RefSeq Version	NM_167574.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chascon (chas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167574.2

ORF Insert Sequence:

ATGGCTTTAA GCAAGACCAT CAAATCAAAA GCTGGCGCCA AAGACGAACA AAACGAACTG 
GTCGAATTCG AACCCAAAGA CGACGAGGAA AGCAACGAGG TCACGGCAAG CAATTGCGAT 
ACAGAAGCTA TTACCAAAGA AGAGGAGTGG ATAACGAAGC GCAAGACAGA ACTAACAACT 
ACCAGACAGA TCGAGACGAG GGTGAAGCGA CAGGTGAAGC TGCAGGATGG CAAAGTGATC 
GAGGATTCGG GGCCGATTGT CTCCACCAAC ACCACCGAGG ACACCGACAA ACAGGAAACG 
GAAACCACTG AGAAACGCGA CCTTGACCTG CCGGATGACC TCGATGGCAA TGCCCAGCTG 
ATCGACGTGG CCGCCCTGCA GGCGGGCGCC ATAAATGAAA CGCAGTTGGC CCAAATTCAA 
GAGGTCTTCG GACCGGCCGA TGGAGCCGGA AAGCTGGTCA AGCGCATGGT TCCGCGTCCG 
GTGGATGGAC TGGTGCGTCA AACGGACGAC AAGAGGGTCA TATCGCACGA GGAGATCAAG 
GACTACCACG AAACGGAGGA CGTACAGCAT CACGGCGACT TTACAGATCA GACCTACGTG 
AACGCAGTTC GCGACGGAGT CGAGAACCTA GAGGCCGTGC TCTGTTCGCC GGAGAGCCAG 
CGGCAGTTGG TGCCGCTCGG ACCCAAGGTG GTTGCCAACC GGAGCAAGTC GCGAAAGACC 
ATCGATTCGG AGGACACACA GAAGCGCTGC CTGGCCCAGG AGGACGGCAC CCTGGTCACC 
GAGAGTCGAC GGACCACCGA GCACGAGTTG ATCGTCGACG ACGAACTGCC CGACCCGCCT 
GCCAACGGCG CCTCCGATGA TCTTGGCCTG CAGCTGCAGG TGGGCGAGCA GGTGACAACG 
ACGGCGGCCA ACCAGCGGTT CTTCCGCCAA CGGGACGAGC AGCATGTGGA CGTGCTAGCC 
AACGGTCGGC TCCAATCCAC CGAGATGCGC TACGCCGCCG AGACCACCCA GATGGACAAG 
GACGGTCCCA CCGATCTGGA GCGACCAGGT GACTGGGACA GTCTATCCGA CCGGATGCGC 
AAGCTTCGCC GATCCCAGCA GCAGCAACAG AAGGAGGTGG CTTTGCTGGC GGATCGCAAG 
GATGCGCTCA CCAAGCGACC CCTGGACTTT GATCGCGAGG AGGAGACGCG CAAGGGCGAG 
ACCATGAAGT GGCTGGAGTC GCACTTTGGC AGCGAGTCCA CCGCCTCCAA CGATTCCCGC 
GACGACGAGG CCGATGCCGA GGTGGAGCCC ACCAAGAAGA GTTACTTCAA TGTGACGATC 
AAGTCGCAGA GCCATGCCCA ATCCCACCTG GCCACCCAGC AGCATTCGCA TCCGCAGCAC 
ATCTCGCATG GCCATCATCC ACAGACTTTG CCCAGAAGCG CCGAGAGGGA ACGGGAGCGC 
GAGAGGGAAC GCGAAAGGGA GCGGGAGAGG GAAAGGGAAC GGGAGCGGGA TAGGGATCGC 
CATCCCCTGG CCATGACCGC ATCCGCCTCG CAGGAGCGCA AGGCACCGGC CAATTCGGCT 
GGCGGTCGCT CCAAGTACTT CCAGGGCATC TCGAACTGGT CGGAACGCAA GGATCCCGGT 
CCGCCGGTCA ATCACTTCTC GTCGCAGGCC TTTCGCGACG ATCTGCAGGA GACCATCCGT 
CGCAATGGCC TCAAGAAGAA GGTCAGCGGT CCCGGCTCCA GTCAGGGATC AGCCCTGGCT 
CAGGAGACGG TGGTCCAGCC CAAGGATAGC TATGTCAGCC GCGAGGATAT ACTGCAGCAA 
AAGCGCCAGA GCCTGTCCTC CCAGTTCCTG GCCCGCTCGA TGCGTGGCTC GCGCGATGCT 
CTGGACGACC TGGAGGCCAT ACCGGGACCG GGACCAGGTC CATCCATGAT ATCACCGCGT 
GCCGAAGAGC CCTCGCACAA ACGGGTGGCC TACGGTCATC GCGGCAACAA TGGCACCCAC 
ATTAATGGCC ATGCTTTGAA TGGAGTGGCT CCCACTCTGG TCAATGGCCG GGGAAAGAGC 
TATGAGCCAC CAGCTCCTCC GCCGGCCGCA GTTCCTCCAC TAGAATTTGG CGGCGGTGGC 
AGCCAGCTGG GCCAAAATCC GCGACCCACT GTCCCGCAGC GACGACGTGC CATCGAAAAG 
AAGCTGGGCG AGCACAGCCA CACCCACAGC CACAGCCACA GCCACTCATC CCACACATCG 
CATAGCGCCC ATCTGCCAGT CAAGTCGCAG GCTCCAATGC CGTCGCAGCT TAACCAATTG 
GAGCCTCCTC CGGACTACTC GCCGCCGCCC AGGAGTCGCA GTCGTTCGTC ATCGCCGCAG 
GTCGATTACC TGATGACCAG GGAGATGCCT GCCAGCACAA TGACGCTCAG CCGGAAGCAG 
CAGCAGCGCA CCCGATTTGC ACCCACATCC AGTTATCCGC CGGCAACTGG AGGTACTGGT 
AACCTCCGGC CACCTGGAGC CACCTCGGTG TCCACTTCGA ATCTATCGGC CATGAGCGGC 
GGCGTCAGTA CGTCCAAGTC GAGCCGCATG GGTCAGGTGA TTGGCAATTC GCTGCGCAAG 
CTGGTCAGCA AAATCCGTTC GGCCAGTGCG GAGCGTAAGT TTCGCATGAA ATCGGCTGCC 
AGCAAGTCGC GCGAGCAATC GCCCAGTCCA GGACAAGATC CGCGGGGCGT TGGTCCTCCG 
GTGACCACCT ATCAGCAGTA CAATGTGATC GATGGGCATA TCGGTAGCCA CGATTCCGAG 
GATTCCAATA GCGACAATCA GCGACGCAGG GAGCGGGAGA GGGAGCGCGA AAGAGAGCGG 
GAACGGGAAC GGGAGCGTGA TCCTCTCAAG CAAGAGTCCC AGTCGCGACA AAATTTCAAG 
CGTGCCGAAT CCGCGGATCC CCAACTGCAG CACCTGGAGC AGGCCATAAG TCCCAGGCAG 
CGGTACTACC TGGGCGAAGA TCCCTACTCC AGCACGCTCT ATGGCAAGGA GAATCACTAC 
GAACGACGAC CACGTCAGCG TAACATGGAG CGCACGGATG AGCGAATGGA ACGATTGGAG 
CGCTTAGAGC GGGAGGATGA CTACTACGAG TCCAGGGCAC CACCACCAGT GACGGCTGCG 
CACACCTTGG GTCGCTACCA GAAGCATGGC CAGCGCTTCA GTGGCTCCAC GCCCAATCTG 
CACCAGCAGC AGCAAGATTA CCGCTCGGCT CAGACGTTGC CACGCAAGCT GCACGAGCAG 
CGCACCCAGA GATTTGGGAA TGCCATGGAG AAGCAACCGG CTCCGGGCAG GGGGAGCTAC 
CTTTCTGCTT CCGGACAACA GCAGCAGTCA AGGGGGATTC CCCATAATCA GAATCAGCAA 
AATCAGCAGA ATCCTAACCC AAGCCCGAGC ATCAACGGCA ATCCAAATCC AATGCAGGCA 
CAGCAAGGAC CGGCCAAGCC GGCTCGCACC TACGCCAAGG TCCTGAACCG CAGCAAGAGC 
TTCAATGTGC ACGGCATGAA CGGCAGCAAC GATCCTAGTC CCATTTACAT CGAGAAGCTC 
ACAAGGAACA ACTATCAGCA GCGTCAGGAG CCGAGCTTTG GCTACGGCCT TGGCCAGGGA 
CCCAATGCCA ATTACAAGTC CAATCCGCAT CTATTCTCTG GCAAGGACAA CTCGCTGAAA 
AGCGGCCTCA AGAGTCCATC GATTGTGAAT CTCATCAGTC GTAGTCAGAA GGACCTAACC 
AAGATTGCTG GTAACGATGA GGATGACGTG GGCTATCCGG AGCAGGACAA GAAGCAGTTG 
TATCTGCGCG GCCTGCACCA GCAGGCGCCA GACCTATACC GGGTGATCCA TGGCGACGAA 
GATTACGGGT CGCGAAAGTA TCCGCTGCAG GCCAAGTATT CGCTGGACTC GCGTTCCCCG 
CCATCCGTGG AGCTCAACAA GGATACGGCG AGCATTGTGC GGCGCAGCAG CGAGGTGGAC 
CACCAGCAGA GCCATCACCA CAACCAGCAG CACCACCAGC ACCACATCCA GAGCCACCAG 
CAGCAGAACC TAAGGCGTGG TTCGGGTGCC ACCATCATTG AGCTGCGCCA TCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chas ( NM_001298452.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285381.1 Drosophila melanogaster chascon (chas), transcript variant D, mRNA.

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CloneID	OFa21520
Clone ID Related Accession (Same CDS sequence)	NM_001298452.1
Accession Version	NM_001298452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chascon, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGTGA TCCTCCTACT GTCGCCATGC ACTTGCAGCC GCAGCAGCAG CAGCAGCCAC 
GCCCACGACC ACGCCCATGA TCCCGGCCAC GCACCATCCA AGCACCACCA CCAATACCAC 
CAGTACCACC AACACCAACA ACACCACCAA AACAGCCAGC ACCATCAACA ACCAGAACAA 
CCACCATCGG TGGCTAGAAG TGGAGATTCC CGCAGCAGCA ATGAGTCTTC ATCGGCATCC 
ACGGGCGACA CAGCCATTCC GGCTGGATAT CTGCAGTTGC ACCACCAGAA ACAGAATCAA 
AATCAAAATC AGAAGGATCC ACAGAATCGG CAACATTCGG ATAATTATCC CCATCAACCT 
AGTCATAGTT GTAGCACCGC CACAAACACA GCACCACTAT CCGTTTTTGG CCACGGATGT 
TGGATGCCCC AGCAGCCAGC GAACGCCGTG CCGAAGCCAC CGCGATCCCA TGGCCTCCAG 
TGGTCATCGA GCGCCTATCC TGCCACACAT GCCACCAGCC ACCAACCGGT CATCTTTCGC 
GTCCAATCGT CACTGCCCAA CGGGAGCTTT ACTAACGCCA CCACCACCAC AACCACCACC 
ACTGACTACG TGCGTCACGT GACCCGCGTT AACTTCTACG ACATTGATAC TTCGCAGGTC 
GAATTCGAAC CCAAAGACGA CGAGGAAAGC AACGAGGTCA CGGCAAGCAA TTGCGATACA 
GAAGCTATTA CCAAAGAAGA GGAGTGGATA ACGAAGCGCA AGACAGAACT AACAACTACC 
AGACAGATCG AGACGAGGGT GAAGCGACAG GTGAAGCTGC AGGATGGCAA AGTGATCGAG 
GATTCGGGGC CGATTGTCTC CACCAACACC ACCGAGGACA CCGACAAACA GGAAACGGAA 
ACCACTGAGA AACGCGACCT TGACCTGCCG GATGACCTCG ATGGCAATGC CCAGCTGATC 
GACGTGGCCG CCCTGCAGGC GGGCGCCATA AATGAAACGC AGTTGGCCCA AATTCAAGAG 
GTCTTCGGAC CGGCCGATGG AGCCGGAAAG CTGGTCAAGC GCATGGTTCC GCGTCCGGTG 
GATGGACTGG TGCGTCAAAC GGACGACAAG AGGGTCATAT CGCACGAGGA GATCAAGGAC 
TACCACGAAA CGGAGGACGT ACAGCATCAC GGCGACTTTA CAGATCAGAC CTACGTGAAC 
GCAGTTCGCG ACGGAGTCGA GAACCTAGAG GCCGTGCTCT GTTCGCCGGA GAGCCAGCGG 
CAGTTGGTGC CGCTCGGACC CAAGGTGGTT GCCAACCGGA GCAAGTCGCG AAAGACCATC 
GATTCGGAGG ACACACAGAA GCGCTGCCTG GCCCAGGAGG ACGGCACCCT GGTCACCGAG 
AGTCGACGGA CCACCGAGCA CGAGTTGATC GTCGACGACG AACTGCCCGA CCCGCCTGCC 
AACGGCGCCT CCGATGATCT TGGCCTGCAG CTGCAGGTGG GCGAGCAGGT GACAACGACG 
GCGGCCAACC AGCGGTTCTT CCGCCAACGG GACGAGCAGC ATGTGGACGT GCTAGCCAAC 
GGTCGGCTCC AATCCACCGA GATGCGCTAC GCCGCCGAGA CCACCCAGAT GGACAAGGAC 
GGTCCCACCG ATCTGGAGCG ACCAGGTGAC TGGGACAGTC TATCCGACCG GATGCGCAAG 
CTTCGCCGAT CCCAGCAGCA GCAACAGAAG GAGGTGGCTT TGCTGGCGGA TCGCAAGGAT 
GCGCTCACCA AGCGACCCCT GGACTTTGAT CGCGAGGAGG AGACGCGCAA GGGCGAGACC 
ATGAAGTGGC TGGAGTCGCA CTTTGGCAGC GAGTCCACCG CCTCCAACGA TTCCCGCGAC 
GACGAGGCCG ATGCCGAGGT GGAGCCCACC AAGAAGAGTT ACTTCAATGT GACGATCAAG 
TCGCAGAGCC ATGCCCAATC CCACCTGGCC ACCCAGCAGC ATTCGCATCC GCAGCACATC 
TCGCATGGCC ATCATCCACA GACTTTGCCC AGAAGCGCCG AGAGGGAACG GGAGCGCGAG 
AGGGAACGCG AAAGGGAGCG GGAGAGGGAA AGGGAACGGG AGCGGGATAG GGATCGCCAT 
CCCCTGGCCA TGACCGCATC CGCCTCGCAG GAGCGCAAGG CACCGGCCAA TTCGGCTGGC 
GGTCGCTCCA AGTACTTCCA GGGCATCTCG AACTGGTCGG AACGCAAGGA TCCCGGTCCG 
CCGGTCAATC ACTTCTCGTC GCAGGCCTTT CGCGACGATC TGCAGGAGAC CATCCGTCGC 
AATGGCCTCA AGAAGAAGGT CAGCGGTCCC GGCTCCAGTC AGGGATCAGC CCTGGCTCAG 
GAGACGGTGG TCCAGCCCAA GGATAGCTAT GTCAGCCGCG AGGATATACT GCAGCAAAAG 
CGCCAGAGCC TGTCCTCCCA GTTCCTGGCC CGCTCGATGC GTGGCTCGCG CGATGCTCTG 
GACGACCTGG AGGCCATACC GGGACCGGGA CCAGGTCCAT CCATGATATC ACCGCGTGCC 
GAAGAGCCCT CGCACAAACG GGTGGCCTAC GGTCATCGCG GCAACAATGG CACCCACATT 
AATGGCCATG CTTTGAATGG AGTGGCTCCC ACTCTGGTCA ATGGCCGGGG AAAGAGCTAT 
GAGCCACCAG CTCCTCCGCC GGCCGCAGTT CCTCCACTAG AATTTGGCGG CGGTGGCAGC 
CAGCTGGGCC AAAATCCGCG ACCCACTGTC CCGCAGCGAC GACGTGCCAT CGAAAAGAAG 
CTGGGCGAGC ACAGCCACAC CCACAGCCAC AGCCACAGCC ACTCATCCCA CACATCGCAT 
AGCGCCCATC TGCCAGTCAA GTCGCAGGCT CCAATGCCGT CGCAGCTTAA CCAATTGGAG 
CCTCCTCCGG ACTACTCGCC GCCGCCCAGG AGTCGCAGTC GTTCGTCATC GCCGCAGGTC 
GATTACCTGA TGACCAGGGA GATGCCTGCC AGCACAATGA CGCTCAGCCG GAAGCAGCAG 
CAGCGCACCC GATTTGCACC CACATCCAGT TATCCGCCGG CAACTGGAGG TACTGGTAAC 
CTCCGGCCAC CTGGAGCCAC CTCGGTGTCC ACTTCGAATC TATCGGCCAT GAGCGGCGGC 
GTCAGTACGT CCAAGTCGAG CCGCATGGGT CAGGTGATTG GCAATTCGCT GCGCAAGCTG 
GTCAGCAAAA TCCGTTCGGC CAGTGCGGAG CGTAAGTTTC GCATGAAATC GGCTGCCAGC 
AAGTCGCGCG AGCAATCGCC CAGTCCAGGA CAAGATCCGC GGGGCGTTGG TCCTCCGGTG 
ACCACCTATC AGCAGTACAA TGTGATCGAT GGGCATATCG GTAGCCACGA TTCCGAGGAT 
TCCAATAGCG ACAATCAGCG ACGCAGGGAG CGGGAGAGGG AGCGCGAAAG AGAGCGGGAA 
CGGGAACGGG AGCGTGATCC TCTCAAGCAA GAGTCCCAGT CGCGACAAAA TTTCAAGCGT 
GCCGAATCCG CGGATCCCCA ACTGCAGCAC CTGGAGCAGG CCATAAGTCC CAGGCAGCGG 
TACTACCTGG GCGAAGATCC CTACTCCAGC ACGCTCTATG GCAAGGAGAA TCACTACGAA 
CGACGACCAC GTCAGCGTAA CATGGAGCGC ACGGATGAGC GAATGGAACG ATTGGAGCGC 
TTAGAGCGGG AGGATGACTA CTACGAGTCC AGGGCACCAC CACCAGTGAC GGCTGCGCAC 
ACCTTGGGTC GCTACCAGAA GCATGGCCAG CGCTTCAGTG GCTCCACGCC CAATCTGCAC 
CAGCAGCAGC AAGATTACCG CTCGGCTCAG ACGTTGCCAC GCAAGCTGCA CGAGCAGCGC 
ACCCAGAGAT TTGGGAATGC CATGGAGAAG CAACCGGCTC CGGGCAGGGG GAGCTACCTT 
TCTGCTTCCG GACAACAGCA GCAGTCAAGG GGGATTCCCC ATAATCAGAA TCAGCAAAAT 
CAGCAGAATC CTAACCCAAG CCCGAGCATC AACGGCAATC CAAATCCAAT GCAGGCACAG 
CAAGGACCGG CCAAGCCGGC TCGCACCTAC GCCAAGGTCC TGAACCGCAG CAAGAGCTTC 
AATGTGCACG GCATGAACGG CAGCAACGAT CCTAGTCCCA TTTACATCGA GAAGCTCACA 
AGGAACAACT ATCAGCAGCG TCAGGAGCCG AGCTTTGGCT ACGGCCTTGG CCAGGGACCC 
AATGCCAATT ACAAGTCCAA TCCGCATCTA TTCTCTGGCA AGGACAACTC GCTGAAAAGC 
GGCCTCAAGA GTCCATCGAT TGTGAATCTC ATCAGTCGTA GTCAGAAGGA CCTAACCAAG 
ATTGCTGGTA ACGATGAGGA TGACGTGGGC TATCCGGAGC AGGACAAGAA GCAGTTGTAT 
CTGCGCGGCC TGCACCAGCA GGCGCCAGAC CTATACCGGG TGATCCATGG CGACGAAGAT 
TACGGGTCGC GAAAGTATCC GCTGCAGGCC AAGTATTCGC TGGACTCGCG TTCCCCGCCA 
TCCGTGGAGC TCAACAAGGA TACGGCGAGC ATTGTGCGGC GCAGCAGCGA GGTGGACCAC 
CAGCAGAGCC ATCACCACAA CCAGCAGCAC CACCAGCACC ACATCCAGAG CCACCAGCAG 
CAGAACCTAA GGCGTGGTTC GGGTGCCACC ATCATTGAGC TGCGCCATCG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285381.1
CDS	373..5049
Translation	MVVILLLSPCTCSRSSSSSHAHDHAHDPGHAPSKHHHQYHQYHQHQQHHQNSQHHQQPEQPPSVARSGDSRSSNESSSASTGDTAIPAGYLQLHHQKQNQNQNQKDPQNRQHSDNYPHQPSHSCSTATNTAPLSVFGHGCWMPQQPANAVPKPPRSHGLQWSSSAYPATHATSHQPVIFRVQSSLPNGSFTNATTTTTTTTDYVRHVTRVNFYDIDTSQVEFEPKDDEESNEVTASNCDTEAITKEEEWITKRKTELTTTRQIETRVKRQVKLQDGKVIEDSGPIVSTNTTEDTDKQETETTEKRDLDLPDDLDGNAQLIDVAALQAGAINETQLAQIQEVFGPADGAGKLVKRMVPRPVDGLVRQTDDKRVISHEEIKDYHETEDVQHHGDFTDQTYVNAVRDGVENLEAVLCSPESQRQLVPLGPKVVANRSKSRKTIDSEDTQKRCLAQEDGTLVTESRRTTEHELIVDDELPDPPANGASDDLGLQLQVGEQVTTTAANQRFFRQRDEQHVDVLANGRLQSTEMRYAAETTQMDKDGPTDLERPGDWDSLSDRMRKLRRSQQQQQKEVALLADRKDALTKRPLDFDREEETRKGETMKWLESHFGSESTASNDSRDDEADAEVEPTKKSYFNVTIKSQSHAQSHLATQQHSHPQHISHGHHPQTLPRSAERERERERERERERERERERERDRDRHPLAMTASASQERKAPANSAGGRSKYFQGISNWSERKDPGPPVNHFSSQAFRDDLQETIRRNGLKKKVSGPGSSQGSALAQETVVQPKDSYVSREDILQQKRQSLSSQFLARSMRGSRDALDDLEAIPGPGPGPSMISPRAEEPSHKRVAYGHRGNNGTHINGHALNGVAPTLVNGRGKSYEPPAPPPAAVPPLEFGGGGSQLGQNPRPTVPQRRRAIEKKLGEHSHTHSHSHSHSSHTSHSAHLPVKSQAPMPSQLNQLEPPPDYSPPPRSRSRSSSPQVDYLMTREMPASTMTLSRKQQQRTRFAPTSSYPPATGGTGNLRPPGATSVSTSNLSAMSGGVSTSKSSRMGQVIGNSLRKLVSKIRSASAERKFRMKSAASKSREQSPSPGQDPRGVGPPVTTYQQYNVIDGHIGSHDSEDSNSDNQRRRERERERERERERERERDPLKQESQSRQNFKRAESADPQLQHLEQAISPRQRYYLGEDPYSSTLYGKENHYERRPRQRNMERTDERMERLERLEREDDYYESRAPPPVTAAHTLGRYQKHGQRFSGSTPNLHQQQQDYRSAQTLPRKLHEQRTQRFGNAMEKQPAPGRGSYLSASGQQQQSRGIPHNQNQQNQQNPNPSPSINGNPNPMQAQQGPAKPARTYAKVLNRSKSFNVHGMNGSNDPSPIYIEKLTRNNYQQRQEPSFGYGLGQGPNANYKSNPHLFSGKDNSLKSGLKSPSIVNLISRSQKDLTKIAGNDEDDVGYPEQDKKQLYLRGLHQQAPDLYRVIHGDEDYGSRKYPLQAKYSLDSRSPPSVELNKDTASIVRRSSEVDHQQSHHHNQQHHQHHIQSHQQQNLRRGSGATIIELRHRK

Target ORF information:

RefSeq Version	NM_001298452.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chascon (chas), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298452.1

ORF Insert Sequence:

ATGGTGGTGA TCCTCCTACT GTCGCCATGC ACTTGCAGCC GCAGCAGCAG CAGCAGCCAC 
GCCCACGACC ACGCCCATGA TCCCGGCCAC GCACCATCCA AGCACCACCA CCAATACCAC 
CAGTACCACC AACACCAACA ACACCACCAA AACAGCCAGC ACCATCAACA ACCAGAACAA 
CCACCATCGG TGGCTAGAAG TGGAGATTCC CGCAGCAGCA ATGAGTCTTC ATCGGCATCC 
ACGGGCGACA CAGCCATTCC GGCTGGATAT CTGCAGTTGC ACCACCAGAA ACAGAATCAA 
AATCAAAATC AGAAGGATCC ACAGAATCGG CAACATTCGG ATAATTATCC CCATCAACCT 
AGTCATAGTT GTAGCACCGC CACAAACACA GCACCACTAT CCGTTTTTGG CCACGGATGT 
TGGATGCCCC AGCAGCCAGC GAACGCCGTG CCGAAGCCAC CGCGATCCCA TGGCCTCCAG 
TGGTCATCGA GCGCCTATCC TGCCACACAT GCCACCAGCC ACCAACCGGT CATCTTTCGC 
GTCCAATCGT CACTGCCCAA CGGGAGCTTT ACTAACGCCA CCACCACCAC AACCACCACC 
ACTGACTACG TGCGTCACGT GACCCGCGTT AACTTCTACG ACATTGATAC TTCGCAGGTC 
GAATTCGAAC CCAAAGACGA CGAGGAAAGC AACGAGGTCA CGGCAAGCAA TTGCGATACA 
GAAGCTATTA CCAAAGAAGA GGAGTGGATA ACGAAGCGCA AGACAGAACT AACAACTACC 
AGACAGATCG AGACGAGGGT GAAGCGACAG GTGAAGCTGC AGGATGGCAA AGTGATCGAG 
GATTCGGGGC CGATTGTCTC CACCAACACC ACCGAGGACA CCGACAAACA GGAAACGGAA 
ACCACTGAGA AACGCGACCT TGACCTGCCG GATGACCTCG ATGGCAATGC CCAGCTGATC 
GACGTGGCCG CCCTGCAGGC GGGCGCCATA AATGAAACGC AGTTGGCCCA AATTCAAGAG 
GTCTTCGGAC CGGCCGATGG AGCCGGAAAG CTGGTCAAGC GCATGGTTCC GCGTCCGGTG 
GATGGACTGG TGCGTCAAAC GGACGACAAG AGGGTCATAT CGCACGAGGA GATCAAGGAC 
TACCACGAAA CGGAGGACGT ACAGCATCAC GGCGACTTTA CAGATCAGAC CTACGTGAAC 
GCAGTTCGCG ACGGAGTCGA GAACCTAGAG GCCGTGCTCT GTTCGCCGGA GAGCCAGCGG 
CAGTTGGTGC CGCTCGGACC CAAGGTGGTT GCCAACCGGA GCAAGTCGCG AAAGACCATC 
GATTCGGAGG ACACACAGAA GCGCTGCCTG GCCCAGGAGG ACGGCACCCT GGTCACCGAG 
AGTCGACGGA CCACCGAGCA CGAGTTGATC GTCGACGACG AACTGCCCGA CCCGCCTGCC 
AACGGCGCCT CCGATGATCT TGGCCTGCAG CTGCAGGTGG GCGAGCAGGT GACAACGACG 
GCGGCCAACC AGCGGTTCTT CCGCCAACGG GACGAGCAGC ATGTGGACGT GCTAGCCAAC 
GGTCGGCTCC AATCCACCGA GATGCGCTAC GCCGCCGAGA CCACCCAGAT GGACAAGGAC 
GGTCCCACCG ATCTGGAGCG ACCAGGTGAC TGGGACAGTC TATCCGACCG GATGCGCAAG 
CTTCGCCGAT CCCAGCAGCA GCAACAGAAG GAGGTGGCTT TGCTGGCGGA TCGCAAGGAT 
GCGCTCACCA AGCGACCCCT GGACTTTGAT CGCGAGGAGG AGACGCGCAA GGGCGAGACC 
ATGAAGTGGC TGGAGTCGCA CTTTGGCAGC GAGTCCACCG CCTCCAACGA TTCCCGCGAC 
GACGAGGCCG ATGCCGAGGT GGAGCCCACC AAGAAGAGTT ACTTCAATGT GACGATCAAG 
TCGCAGAGCC ATGCCCAATC CCACCTGGCC ACCCAGCAGC ATTCGCATCC GCAGCACATC 
TCGCATGGCC ATCATCCACA GACTTTGCCC AGAAGCGCCG AGAGGGAACG GGAGCGCGAG 
AGGGAACGCG AAAGGGAGCG GGAGAGGGAA AGGGAACGGG AGCGGGATAG GGATCGCCAT 
CCCCTGGCCA TGACCGCATC CGCCTCGCAG GAGCGCAAGG CACCGGCCAA TTCGGCTGGC 
GGTCGCTCCA AGTACTTCCA GGGCATCTCG AACTGGTCGG AACGCAAGGA TCCCGGTCCG 
CCGGTCAATC ACTTCTCGTC GCAGGCCTTT CGCGACGATC TGCAGGAGAC CATCCGTCGC 
AATGGCCTCA AGAAGAAGGT CAGCGGTCCC GGCTCCAGTC AGGGATCAGC CCTGGCTCAG 
GAGACGGTGG TCCAGCCCAA GGATAGCTAT GTCAGCCGCG AGGATATACT GCAGCAAAAG 
CGCCAGAGCC TGTCCTCCCA GTTCCTGGCC CGCTCGATGC GTGGCTCGCG CGATGCTCTG 
GACGACCTGG AGGCCATACC GGGACCGGGA CCAGGTCCAT CCATGATATC ACCGCGTGCC 
GAAGAGCCCT CGCACAAACG GGTGGCCTAC GGTCATCGCG GCAACAATGG CACCCACATT 
AATGGCCATG CTTTGAATGG AGTGGCTCCC ACTCTGGTCA ATGGCCGGGG AAAGAGCTAT 
GAGCCACCAG CTCCTCCGCC GGCCGCAGTT CCTCCACTAG AATTTGGCGG CGGTGGCAGC 
CAGCTGGGCC AAAATCCGCG ACCCACTGTC CCGCAGCGAC GACGTGCCAT CGAAAAGAAG 
CTGGGCGAGC ACAGCCACAC CCACAGCCAC AGCCACAGCC ACTCATCCCA CACATCGCAT 
AGCGCCCATC TGCCAGTCAA GTCGCAGGCT CCAATGCCGT CGCAGCTTAA CCAATTGGAG 
CCTCCTCCGG ACTACTCGCC GCCGCCCAGG AGTCGCAGTC GTTCGTCATC GCCGCAGGTC 
GATTACCTGA TGACCAGGGA GATGCCTGCC AGCACAATGA CGCTCAGCCG GAAGCAGCAG 
CAGCGCACCC GATTTGCACC CACATCCAGT TATCCGCCGG CAACTGGAGG TACTGGTAAC 
CTCCGGCCAC CTGGAGCCAC CTCGGTGTCC ACTTCGAATC TATCGGCCAT GAGCGGCGGC 
GTCAGTACGT CCAAGTCGAG CCGCATGGGT CAGGTGATTG GCAATTCGCT GCGCAAGCTG 
GTCAGCAAAA TCCGTTCGGC CAGTGCGGAG CGTAAGTTTC GCATGAAATC GGCTGCCAGC 
AAGTCGCGCG AGCAATCGCC CAGTCCAGGA CAAGATCCGC GGGGCGTTGG TCCTCCGGTG 
ACCACCTATC AGCAGTACAA TGTGATCGAT GGGCATATCG GTAGCCACGA TTCCGAGGAT 
TCCAATAGCG ACAATCAGCG ACGCAGGGAG CGGGAGAGGG AGCGCGAAAG AGAGCGGGAA 
CGGGAACGGG AGCGTGATCC TCTCAAGCAA GAGTCCCAGT CGCGACAAAA TTTCAAGCGT 
GCCGAATCCG CGGATCCCCA ACTGCAGCAC CTGGAGCAGG CCATAAGTCC CAGGCAGCGG 
TACTACCTGG GCGAAGATCC CTACTCCAGC ACGCTCTATG GCAAGGAGAA TCACTACGAA 
CGACGACCAC GTCAGCGTAA CATGGAGCGC ACGGATGAGC GAATGGAACG ATTGGAGCGC 
TTAGAGCGGG AGGATGACTA CTACGAGTCC AGGGCACCAC CACCAGTGAC GGCTGCGCAC 
ACCTTGGGTC GCTACCAGAA GCATGGCCAG CGCTTCAGTG GCTCCACGCC CAATCTGCAC 
CAGCAGCAGC AAGATTACCG CTCGGCTCAG ACGTTGCCAC GCAAGCTGCA CGAGCAGCGC 
ACCCAGAGAT TTGGGAATGC CATGGAGAAG CAACCGGCTC CGGGCAGGGG GAGCTACCTT 
TCTGCTTCCG GACAACAGCA GCAGTCAAGG GGGATTCCCC ATAATCAGAA TCAGCAAAAT 
CAGCAGAATC CTAACCCAAG CCCGAGCATC AACGGCAATC CAAATCCAAT GCAGGCACAG 
CAAGGACCGG CCAAGCCGGC TCGCACCTAC GCCAAGGTCC TGAACCGCAG CAAGAGCTTC 
AATGTGCACG GCATGAACGG CAGCAACGAT CCTAGTCCCA TTTACATCGA GAAGCTCACA 
AGGAACAACT ATCAGCAGCG TCAGGAGCCG AGCTTTGGCT ACGGCCTTGG CCAGGGACCC 
AATGCCAATT ACAAGTCCAA TCCGCATCTA TTCTCTGGCA AGGACAACTC GCTGAAAAGC 
GGCCTCAAGA GTCCATCGAT TGTGAATCTC ATCAGTCGTA GTCAGAAGGA CCTAACCAAG 
ATTGCTGGTA ACGATGAGGA TGACGTGGGC TATCCGGAGC AGGACAAGAA GCAGTTGTAT 
CTGCGCGGCC TGCACCAGCA GGCGCCAGAC CTATACCGGG TGATCCATGG CGACGAAGAT 
TACGGGTCGC GAAAGTATCC GCTGCAGGCC AAGTATTCGC TGGACTCGCG TTCCCCGCCA 
TCCGTGGAGC TCAACAAGGA TACGGCGAGC ATTGTGCGGC GCAGCAGCGA GGTGGACCAC 
CAGCAGAGCC ATCACCACAA CCAGCAGCAC CACCAGCACC ACATCCAGAG CCACCAGCAG 
CAGAACCTAA GGCGTGGTTC GGGTGCCACC ATCATTGAGC TGCGCCATCG CAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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chas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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