ppk28 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk28
Entrez Gene ID	32671
Full Name	pickpocket 28
Synonyms	CG4805,Dmel\CG4805,PPK28
General protein information	Preferred Names pickpocket 28 Names CG4805 gene product from transcript CG4805-RA CG4805-PA ppk28-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 15A9-15A10

mRNA and Protein(s)

mRNA	Protein	Name
NM_132941.3	NP_573169.2	pickpocket 28

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001602	XP_321501.2
Drosophila melanogaster (fruit fly)	ppk28	NP_573169.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that loss of the critical water sensor, pickpocket 28 (ppk28), altered metabolic homeostasis to promote internal lipid and water stores and extended healthy lifespan.
Title: Water sensor ppk28 modulates Drosophila lifespan and physiology through AKH signaling.

a member of the degenerin/epithelial sodium channel family, PPK28, as an osmosensitive ion channel that mediates the cellular and behavioural response to water
Title: The molecular basis for water taste in Drosophila.

the amiloride-sensitive PPK28 channel may serve as the osmolarity sensor for gustatory water reception in the fruit fly.
Title: The amiloride-sensitive epithelial Na+ channel PPK28 is essential for drosophila gustatory water reception.

The following ppk28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01840	NM_132941.3 Latest version!	Drosophila melanogaster pickpocket 28 (ppk28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk28 ( NM_132941.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573169.2 Drosophila melanogaster pickpocket 28 (ppk28), mRNA.

Price & Availability↑

CloneID	OFa01840
Clone ID Related Accession (Same CDS sequence)	NM_132941.3
Accession Version	NM_132941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pickpocket 28
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132941.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGGACGT TGACGGAAAG CAGGCGGCGC CAAAGTGGCT CCTCCGGGTG CAAAAAGGAC 
TCCGAGTCGG ATGACGATGA GAATACCATC TGTAGCAGGG CGGCCATTAA GAGGAGTGTG 
GTCTACTATC TCAAGAACAG CACCCTCCAT GGATTAAAGT ACATCGCCGA AGAGAGCATC 
ACCATTCCGG AACGCATCTT TTTTGGTCTT GCCTTTGTGC TGGTGGTGAT CCTTTCCGTG 
TTCTTCATAT CCAACGTGTA CGTAAAGTGG AGTGCTTCGC CCATAATCAT TTCGACCAGT 
GCCAAGCAAA AGCTGACCAG CAACATGCCA TTTCCGGCGA TCACGATCTG TAATCTCAAC 
CAGGCGCTGC TAAGCAAAGT GGATCGCATT GGACGGACCA GCACGAATTT CTCGCTGCTG 
ATGGGTCTGT GTGACCAGGG CGGGGACACG ACCATCTCGT ACATCGGCAC CTGGAAGTAC 
TTCAAGGCGA TCCTGGTGGA CGTGGCCCAG CCGTGCGAGA AAATGCTCCT GTACTGCAGC 
TTCGGATCGC GCGAGGAGGA CTGCTCCTGG CTCTTCACCT CCATACTGAC GGACGATGGG 
CTGTGCTGCA ACTTCAATGC CCTGCATCCG TCGTATCTTA TACGGAACTA CAGTGACGAT 
GTGCGGCTGG AGACGGCTCA TCCGAATACC CGTTATGAGC TCATCGATTG GACACCAGAG 
AAGGGTTATG CCCGCAATTT GCCGGAGTTC TACTTTCCGC GCACCTCGGG GGGCACGGGC 
ATCCGCATGG GTCTCACCGT CGTGCTGAAC GCCTCCATAG CGGAGTATTA CTGCACCAAG 
TCGATGAGTG TTGGTTTCAA GGTTCTTGTG CACAATCCCG CCGAGCTGCC AAAAGTGAGC 
AACTATGGCT TCGTTGTGAC CGCGGGACGC GAGGCACGGA TACCCATCGA ACCAGTCTAT 
GAGGATGCCC TGCCCACGAT ACGGTCAATT AAGAAATCGG TGCGTCGCTG CCTCTTCTCC 
GATGAGAATG ACCTGGCCTA CTATCGGACA TATTCGCGTA AGAACTGCGA GCTGGAATGC 
GAGGCGAAGT TGCTGCTACG CGAGTGCAGC TGTGTGCTGT ACTATCTGCC CCGGATCGAT 
CCGTTGGCCA GGGTGTGCGG ACCCAACGAT AATCAGTGCA CGGATCGGGT GCAAACCGAG 
ATTGAGTCAT CGCTGACGAA TCTGTCCTGC GAGAACTGCT GGCCGGGTTG CTTCGAGCTA 
ACATACAGGG CCACACTTTC AACGGCTTCG ATCGTTTCGG ATCCACGATT TCAGGCCGGC 
GAGAATCTAC CCGAATATAT CTTCCACGGG CCATATAGCA ATGCCAGCGA GATTTCGATA 
TTGCACTTCT ACTACATGAC CAACATATTT CGCAGCACCA CCAAGTCCGA GATGTTTGGC 
TTTACCGAAT TCTTGTCTAA TACGGGCGGC CTTTTGGGAC TTTTCATGGG CTTCAGCATT 
TTTTCGGTGA TCGAGATCTT CTTCTACATC ACCGTCCGTC CGTACTGCGC CTCGCGAACA 
CTCCGGCAGC GCCACAAACG CCGCCTGGAG CAGCTGAGCT GGCTGACGCC GATCAGGATG 
CCCGTGCGCA GAGCACTGCG TCGCAATCGA GGTGGTCTCC TCCGGAATCC GCCGCCGCCG 
GCGTACAGTG ATCTGCAAAA GTTCCGCGGG AAACTGGACA AGCCGGAGAC ATGCAAGCGG 
AAACTTTGGC GGACGCTGCA AGTGCGGCCA GTAGTGGATC GGGCGGATGA GGAACTGCCC 
ACCTATCCCT ACCTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573169.2
CDS	240..2060
Translation	MRTLTESRRRQSGSSGCKKDSESDDDENTICSRAAIKRSVVYYLKNSTLHGLKYIAEESITIPERIFFGLAFVLVVILSVFFISNVYVKWSASPIIISTSAKQKLTSNMPFPAITICNLNQALLSKVDRIGRTSTNFSLLMGLCDQGGDTTISYIGTWKYFKAILVDVAQPCEKMLLYCSFGSREEDCSWLFTSILTDDGLCCNFNALHPSYLIRNYSDDVRLETAHPNTRYELIDWTPEKGYARNLPEFYFPRTSGGTGIRMGLTVVLNASIAEYYCTKSMSVGFKVLVHNPAELPKVSNYGFVVTAGREARIPIEPVYEDALPTIRSIKKSVRRCLFSDENDLAYYRTYSRKNCELECEAKLLLRECSCVLYYLPRIDPLARVCGPNDNQCTDRVQTEIESSLTNLSCENCWPGCFELTYRATLSTASIVSDPRFQAGENLPEYIFHGPYSNASEISILHFYYMTNIFRSTTKSEMFGFTEFLSNTGGLLGLFMGFSIFSVIEIFFYITVRPYCASRTLRQRHKRRLEQLSWLTPIRMPVRRALRRNRGGLLRNPPPPAYSDLQKFRGKLDKPETCKRKLWRTLQVRPVVDRADEELPTYPYLD

Target ORF information:

RefSeq Version	NM_132941.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pickpocket 28 (ppk28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132941.3

ORF Insert Sequence:

ATGAGGACGT TGACGGAAAG CAGGCGGCGC CAAAGTGGCT CCTCCGGGTG CAAAAAGGAC 
TCCGAGTCGG ATGACGATGA GAATACCATC TGTAGCAGGG CGGCCATTAA GAGGAGTGTG 
GTCTACTATC TCAAGAACAG CACCCTCCAT GGATTAAAGT ACATCGCCGA AGAGAGCATC 
ACCATTCCGG AACGCATCTT TTTTGGTCTT GCCTTTGTGC TGGTGGTGAT CCTTTCCGTG 
TTCTTCATAT CCAACGTGTA CGTAAAGTGG AGTGCTTCGC CCATAATCAT TTCGACCAGT 
GCCAAGCAAA AGCTGACCAG CAACATGCCA TTTCCGGCGA TCACGATCTG TAATCTCAAC 
CAGGCGCTGC TAAGCAAAGT GGATCGCATT GGACGGACCA GCACGAATTT CTCGCTGCTG 
ATGGGTCTGT GTGACCAGGG CGGGGACACG ACCATCTCGT ACATCGGCAC CTGGAAGTAC 
TTCAAGGCGA TCCTGGTGGA CGTGGCCCAG CCGTGCGAGA AAATGCTCCT GTACTGCAGC 
TTCGGATCGC GCGAGGAGGA CTGCTCCTGG CTCTTCACCT CCATACTGAC GGACGATGGG 
CTGTGCTGCA ACTTCAATGC CCTGCATCCG TCGTATCTTA TACGGAACTA CAGTGACGAT 
GTGCGGCTGG AGACGGCTCA TCCGAATACC CGTTATGAGC TCATCGATTG GACACCAGAG 
AAGGGTTATG CCCGCAATTT GCCGGAGTTC TACTTTCCGC GCACCTCGGG GGGCACGGGC 
ATCCGCATGG GTCTCACCGT CGTGCTGAAC GCCTCCATAG CGGAGTATTA CTGCACCAAG 
TCGATGAGTG TTGGTTTCAA GGTTCTTGTG CACAATCCCG CCGAGCTGCC AAAAGTGAGC 
AACTATGGCT TCGTTGTGAC CGCGGGACGC GAGGCACGGA TACCCATCGA ACCAGTCTAT 
GAGGATGCCC TGCCCACGAT ACGGTCAATT AAGAAATCGG TGCGTCGCTG CCTCTTCTCC 
GATGAGAATG ACCTGGCCTA CTATCGGACA TATTCGCGTA AGAACTGCGA GCTGGAATGC 
GAGGCGAAGT TGCTGCTACG CGAGTGCAGC TGTGTGCTGT ACTATCTGCC CCGGATCGAT 
CCGTTGGCCA GGGTGTGCGG ACCCAACGAT AATCAGTGCA CGGATCGGGT GCAAACCGAG 
ATTGAGTCAT CGCTGACGAA TCTGTCCTGC GAGAACTGCT GGCCGGGTTG CTTCGAGCTA 
ACATACAGGG CCACACTTTC AACGGCTTCG ATCGTTTCGG ATCCACGATT TCAGGCCGGC 
GAGAATCTAC CCGAATATAT CTTCCACGGG CCATATAGCA ATGCCAGCGA GATTTCGATA 
TTGCACTTCT ACTACATGAC CAACATATTT CGCAGCACCA CCAAGTCCGA GATGTTTGGC 
TTTACCGAAT TCTTGTCTAA TACGGGCGGC CTTTTGGGAC TTTTCATGGG CTTCAGCATT 
TTTTCGGTGA TCGAGATCTT CTTCTACATC ACCGTCCGTC CGTACTGCGC CTCGCGAACA 
CTCCGGCAGC GCCACAAACG CCGCCTGGAG CAGCTGAGCT GGCTGACGCC GATCAGGATG 
CCCGTGCGCA GAGCACTGCG TCGCAATCGA GGTGGTCTCC TCCGGAATCC GCCGCCGCCG 
GCGTACAGTG ATCTGCAAAA GTTCCGCGGG AAACTGGACA AGCCGGAGAC ATGCAAGCGG 
AAACTTTGGC GGACGCTGCA AGTGCGGCCA GTAGTGGATC GGGCGGATGA GGAACTGCCC 
ACCTATCCCT ACCTGGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ppk28 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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