mthl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mthl1
Entrez Gene ID	32637
Full Name	methuselah-like 1
Synonyms	CG4521,Dmel\CG4521,Mist,Mth-1,anon-WO0170980.22,anon-WO0170980.23,mist
General protein information	Preferred Names methuselah-like 1 Names CG4521 gene product from transcript CG4521-RB CG4521-PA CG4521-PB mesoderm-invagination signal transducer mth-like 1 mthl1-PA mthl1-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14F4-14F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298411.1	NP_001285340.1	methuselah-like 1, isoform B
NM_132912.2	NP_573140.1	methuselah-like 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	mthl1	NP_573140.1
Anopheles gambiae (African malaria mosquito)	GPRMTH3	XP_307871.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mthl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mthl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03043	NM_132912.2 Latest version!	Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03043	NM_001298411.1 Latest version!	Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mthl1 ( NM_001298411.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285340.1 Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03043
Clone ID Related Accession (Same CDS sequence)	NM_001298411.1 , NM_132912.2
Accession Version	NM_001298411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	methuselah-like 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGGA GTCGGAGTAG CAGAGCTGCC AGCAGCAACC AGTTCATCAG GCCATGTGGC 
CTACTGACGA CCGTCATCCT GCTGCAGACG CTTGTGTCGA TGAGTTTGGC CATCGAGGAG 
ATGTCGCCAC CACCGGCCGC CCCGCCACGT CCATCGCCGC CGCCCACCGT GAAGCTCAAC 
AAGTGCTGCC ATTCGGGCGA GTATCTGAAC GATGGCACCT GCATCGCCGG CAGCGAAGCC 
CTGTGGCTGC CCATGGTGTA CTTGGTGCAG CAGCAGCGCT TCTTCGAGCC CCATGGCGCC 
AGTCCGCGAT TCTTGAAGTT CCTGCCCAAT ACGAGACCCA CGTGCCGAAA GGACCAGACG 
ACGGAGATCT TTCGCAGCCG CGGAGCCAAT GTGATGCTCT TTCCCAATGG CACGCTCTAT 
GTGCGGGAAC GCGCTCTAAT GGTCCAGCCG TCGGATTACT GTGTCGACTG GGAGGTGGCC 
GTCGTTTGCT TGAACGACAG TCAGCCGATC AATGCTCTGG AGGATCCCGA TTATGCAGCC 
AATCCTCTCG TGCAGCAGGA GCCGCCCAAG GCTAGCCTGC GACTGAGCAA GTGCTGCGGC 
AAGTGGGGCA GCTATAACAC GCAGCTCCAG AACTGCGATC TGCAGCCCAA CCATCAGGCA 
GCCGTCGATG GACTGCTGCG ATTGTCGCCG CAGCTACCGG AGGGCAGCTA TCAGACTAGC 
TACGGCCTGC CCGACTGTGG CCAACCCGGT GGCTACTCCA TTGCCGGTGA TTGGCAGGAT 
GCCAAGCTGG ATCGGAACAC GGCCATGCTC CAGTTGCCGC ACAAGAACCT CAGCGCCGGA 
CAATACTGCC TGGAGCACAC GCAGCGCGAG GGCGAGGTGA AGATCATAGC CTGCCAGCAT 
TTGTTTAGCT CCGCGGCAGG AGCCGGCATC CACGACGGCT CCATTGGAGG CACCATCGAG 
CAGGCCAATG GCCAGAATCT GCAGAAGGCC GTCCTGACTG GTGGCATACT TGTGTCCATT 
GTCTTCCTGT CCGCCACTTT GGTGGCCGGC TTCCTGCTGC CCGCTGTGCA TCATGCGCTT 
CATTGGCGCT GCCAAATCTG CTATGTCACC TGCTTGCTAT TCGGCAAAAT ACTGCTGGCC 
ATCGAGGAGC TGAGCTCCAG CCTGCAGCCG GGCAGTGCCG CCTGCCATAC GCTTGCTATC 
ACCATGCAGT TCTTCTTTCT GGCCGCCTTC TTTTGGCTGA ACACCATGTG CTTCAACATC 
TGGTGGACGT TCCGGGACTT TCGGCCCAGT TCGTTGGAAC GCAATCAGGA GGCACTGCGT 
CGCTATCTGT ACTCCCTGTA CGCTTGGGGC GGTCCTCTGC TTATCACCTT CGTGGCCGCC 
TGCGTGGACC AGCTGCCGGA GACGACGCTA CTGCGTCCGG GATTCGGACA GCTCTACTGC 
TGGTTCGACA ATCGCAATCT CTCGATCTTC GCCTACTTCT ACGGACCCAT TGGCCTGCTG 
CTGTGCGCCA ATATAGCGCT CTTCGTGTCC ACCACCCATC AGTTGACGTG CGGCCTGTGG 
AAACGGGACG ATGTCAAGTC CTCATCGGAG AAGTCCGCCC TGGGACGCGT CTGCCTGAAG 
CTAGTCGTCG TTATGGGTGT CACCTGGATA GCGGACATTC TGTCCTGGCT AGTGGGTGGA 
CCGCATGGCG TATGGTTCTT CACCGACCTG ATCAACGCCC TGCAAGGCGT CTTCATCTTC 
ATCGTGGTGG GCTGCCAGCC GCAGGTGTGG ACCGCCTGCC GCAGGATCTT CTGTCCGCGA 
TTGCGTCATG ACATCACCAA TACGACCAAC GGTGTCCAGC ATTCGAGCAG CTCGCAGGGT 
TTGCCCTCGA TGGCCGGCGG CACGGAGATT ACACAGAACA CCACCACCAC CACCACGACA 
ACCAACACGA CCGCCACTCA CATGCCCAGC AATCCGGCGG AGGATGAGGT ACCGGAGAAG 
GCTCCCATTG CCCCCGTCGC ACCCATCGTC AAAATGGAGA CCATTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285340.1
CDS	510..2540
Translation	MDRSRSSRAASSNQFIRPCGLLTTVILLQTLVSMSLAIEEMSPPPAAPPRPSPPPTVKLNKCCHSGEYLNDGTCIAGSEALWLPMVYLVQQQRFFEPHGASPRFLKFLPNTRPTCRKDQTTEIFRSRGANVMLFPNGTLYVRERALMVQPSDYCVDWEVAVVCLNDSQPINALEDPDYAANPLVQQEPPKASLRLSKCCGKWGSYNTQLQNCDLQPNHQAAVDGLLRLSPQLPEGSYQTSYGLPDCGQPGGYSIAGDWQDAKLDRNTAMLQLPHKNLSAGQYCLEHTQREGEVKIIACQHLFSSAAGAGIHDGSIGGTIEQANGQNLQKAVLTGGILVSIVFLSATLVAGFLLPAVHHALHWRCQICYVTCLLFGKILLAIEELSSSLQPGSAACHTLAITMQFFFLAAFFWLNTMCFNIWWTFRDFRPSSLERNQEALRRYLYSLYAWGGPLLITFVAACVDQLPETTLLRPGFGQLYCWFDNRNLSIFAYFYGPIGLLLCANIALFVSTTHQLTCGLWKRDDVKSSSEKSALGRVCLKLVVVMGVTWIADILSWLVGGPHGVWFFTDLINALQGVFIFIVVGCQPQVWTACRRIFCPRLRHDITNTTNGVQHSSSSQGLPSMAGGTEITQNTTTTTTTTNTTATHMPSNPAEDEVPEKAPIAPVAPIVKMETIC

Target ORF information:

RefSeq Version	NM_001298411.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298411.1

ORF Insert Sequence:

ATGGACAGGA GTCGGAGTAG CAGAGCTGCC AGCAGCAACC AGTTCATCAG GCCATGTGGC 
CTACTGACGA CCGTCATCCT GCTGCAGACG CTTGTGTCGA TGAGTTTGGC CATCGAGGAG 
ATGTCGCCAC CACCGGCCGC CCCGCCACGT CCATCGCCGC CGCCCACCGT GAAGCTCAAC 
AAGTGCTGCC ATTCGGGCGA GTATCTGAAC GATGGCACCT GCATCGCCGG CAGCGAAGCC 
CTGTGGCTGC CCATGGTGTA CTTGGTGCAG CAGCAGCGCT TCTTCGAGCC CCATGGCGCC 
AGTCCGCGAT TCTTGAAGTT CCTGCCCAAT ACGAGACCCA CGTGCCGAAA GGACCAGACG 
ACGGAGATCT TTCGCAGCCG CGGAGCCAAT GTGATGCTCT TTCCCAATGG CACGCTCTAT 
GTGCGGGAAC GCGCTCTAAT GGTCCAGCCG TCGGATTACT GTGTCGACTG GGAGGTGGCC 
GTCGTTTGCT TGAACGACAG TCAGCCGATC AATGCTCTGG AGGATCCCGA TTATGCAGCC 
AATCCTCTCG TGCAGCAGGA GCCGCCCAAG GCTAGCCTGC GACTGAGCAA GTGCTGCGGC 
AAGTGGGGCA GCTATAACAC GCAGCTCCAG AACTGCGATC TGCAGCCCAA CCATCAGGCA 
GCCGTCGATG GACTGCTGCG ATTGTCGCCG CAGCTACCGG AGGGCAGCTA TCAGACTAGC 
TACGGCCTGC CCGACTGTGG CCAACCCGGT GGCTACTCCA TTGCCGGTGA TTGGCAGGAT 
GCCAAGCTGG ATCGGAACAC GGCCATGCTC CAGTTGCCGC ACAAGAACCT CAGCGCCGGA 
CAATACTGCC TGGAGCACAC GCAGCGCGAG GGCGAGGTGA AGATCATAGC CTGCCAGCAT 
TTGTTTAGCT CCGCGGCAGG AGCCGGCATC CACGACGGCT CCATTGGAGG CACCATCGAG 
CAGGCCAATG GCCAGAATCT GCAGAAGGCC GTCCTGACTG GTGGCATACT TGTGTCCATT 
GTCTTCCTGT CCGCCACTTT GGTGGCCGGC TTCCTGCTGC CCGCTGTGCA TCATGCGCTT 
CATTGGCGCT GCCAAATCTG CTATGTCACC TGCTTGCTAT TCGGCAAAAT ACTGCTGGCC 
ATCGAGGAGC TGAGCTCCAG CCTGCAGCCG GGCAGTGCCG CCTGCCATAC GCTTGCTATC 
ACCATGCAGT TCTTCTTTCT GGCCGCCTTC TTTTGGCTGA ACACCATGTG CTTCAACATC 
TGGTGGACGT TCCGGGACTT TCGGCCCAGT TCGTTGGAAC GCAATCAGGA GGCACTGCGT 
CGCTATCTGT ACTCCCTGTA CGCTTGGGGC GGTCCTCTGC TTATCACCTT CGTGGCCGCC 
TGCGTGGACC AGCTGCCGGA GACGACGCTA CTGCGTCCGG GATTCGGACA GCTCTACTGC 
TGGTTCGACA ATCGCAATCT CTCGATCTTC GCCTACTTCT ACGGACCCAT TGGCCTGCTG 
CTGTGCGCCA ATATAGCGCT CTTCGTGTCC ACCACCCATC AGTTGACGTG CGGCCTGTGG 
AAACGGGACG ATGTCAAGTC CTCATCGGAG AAGTCCGCCC TGGGACGCGT CTGCCTGAAG 
CTAGTCGTCG TTATGGGTGT CACCTGGATA GCGGACATTC TGTCCTGGCT AGTGGGTGGA 
CCGCATGGCG TATGGTTCTT CACCGACCTG ATCAACGCCC TGCAAGGCGT CTTCATCTTC 
ATCGTGGTGG GCTGCCAGCC GCAGGTGTGG ACCGCCTGCC GCAGGATCTT CTGTCCGCGA 
TTGCGTCATG ACATCACCAA TACGACCAAC GGTGTCCAGC ATTCGAGCAG CTCGCAGGGT 
TTGCCCTCGA TGGCCGGCGG CACGGAGATT ACACAGAACA CCACCACCAC CACCACGACA 
ACCAACACGA CCGCCACTCA CATGCCCAGC AATCCGGCGG AGGATGAGGT ACCGGAGAAG 
GCTCCCATTG CCCCCGTCGC ACCCATCGTC AAAATGGAGA CCATTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mthl1 ( NM_132912.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573140.1 Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03043
Clone ID Related Accession (Same CDS sequence)	NM_001298411.1 , NM_132912.2
Accession Version	NM_132912.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	methuselah-like 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132912.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGGA GTCGGAGTAG CAGAGCTGCC AGCAGCAACC AGTTCATCAG GCCATGTGGC 
CTACTGACGA CCGTCATCCT GCTGCAGACG CTTGTGTCGA TGAGTTTGGC CATCGAGGAG 
ATGTCGCCAC CACCGGCCGC CCCGCCACGT CCATCGCCGC CGCCCACCGT GAAGCTCAAC 
AAGTGCTGCC ATTCGGGCGA GTATCTGAAC GATGGCACCT GCATCGCCGG CAGCGAAGCC 
CTGTGGCTGC CCATGGTGTA CTTGGTGCAG CAGCAGCGCT TCTTCGAGCC CCATGGCGCC 
AGTCCGCGAT TCTTGAAGTT CCTGCCCAAT ACGAGACCCA CGTGCCGAAA GGACCAGACG 
ACGGAGATCT TTCGCAGCCG CGGAGCCAAT GTGATGCTCT TTCCCAATGG CACGCTCTAT 
GTGCGGGAAC GCGCTCTAAT GGTCCAGCCG TCGGATTACT GTGTCGACTG GGAGGTGGCC 
GTCGTTTGCT TGAACGACAG TCAGCCGATC AATGCTCTGG AGGATCCCGA TTATGCAGCC 
AATCCTCTCG TGCAGCAGGA GCCGCCCAAG GCTAGCCTGC GACTGAGCAA GTGCTGCGGC 
AAGTGGGGCA GCTATAACAC GCAGCTCCAG AACTGCGATC TGCAGCCCAA CCATCAGGCA 
GCCGTCGATG GACTGCTGCG ATTGTCGCCG CAGCTACCGG AGGGCAGCTA TCAGACTAGC 
TACGGCCTGC CCGACTGTGG CCAACCCGGT GGCTACTCCA TTGCCGGTGA TTGGCAGGAT 
GCCAAGCTGG ATCGGAACAC GGCCATGCTC CAGTTGCCGC ACAAGAACCT CAGCGCCGGA 
CAATACTGCC TGGAGCACAC GCAGCGCGAG GGCGAGGTGA AGATCATAGC CTGCCAGCAT 
TTGTTTAGCT CCGCGGCAGG AGCCGGCATC CACGACGGCT CCATTGGAGG CACCATCGAG 
CAGGCCAATG GCCAGAATCT GCAGAAGGCC GTCCTGACTG GTGGCATACT TGTGTCCATT 
GTCTTCCTGT CCGCCACTTT GGTGGCCGGC TTCCTGCTGC CCGCTGTGCA TCATGCGCTT 
CATTGGCGCT GCCAAATCTG CTATGTCACC TGCTTGCTAT TCGGCAAAAT ACTGCTGGCC 
ATCGAGGAGC TGAGCTCCAG CCTGCAGCCG GGCAGTGCCG CCTGCCATAC GCTTGCTATC 
ACCATGCAGT TCTTCTTTCT GGCCGCCTTC TTTTGGCTGA ACACCATGTG CTTCAACATC 
TGGTGGACGT TCCGGGACTT TCGGCCCAGT TCGTTGGAAC GCAATCAGGA GGCACTGCGT 
CGCTATCTGT ACTCCCTGTA CGCTTGGGGC GGTCCTCTGC TTATCACCTT CGTGGCCGCC 
TGCGTGGACC AGCTGCCGGA GACGACGCTA CTGCGTCCGG GATTCGGACA GCTCTACTGC 
TGGTTCGACA ATCGCAATCT CTCGATCTTC GCCTACTTCT ACGGACCCAT TGGCCTGCTG 
CTGTGCGCCA ATATAGCGCT CTTCGTGTCC ACCACCCATC AGTTGACGTG CGGCCTGTGG 
AAACGGGACG ATGTCAAGTC CTCATCGGAG AAGTCCGCCC TGGGACGCGT CTGCCTGAAG 
CTAGTCGTCG TTATGGGTGT CACCTGGATA GCGGACATTC TGTCCTGGCT AGTGGGTGGA 
CCGCATGGCG TATGGTTCTT CACCGACCTG ATCAACGCCC TGCAAGGCGT CTTCATCTTC 
ATCGTGGTGG GCTGCCAGCC GCAGGTGTGG ACCGCCTGCC GCAGGATCTT CTGTCCGCGA 
TTGCGTCATG ACATCACCAA TACGACCAAC GGTGTCCAGC ATTCGAGCAG CTCGCAGGGT 
TTGCCCTCGA TGGCCGGCGG CACGGAGATT ACACAGAACA CCACCACCAC CACCACGACA 
ACCAACACGA CCGCCACTCA CATGCCCAGC AATCCGGCGG AGGATGAGGT ACCGGAGAAG 
GCTCCCATTG CCCCCGTCGC ACCCATCGTC AAAATGGAGA CCATTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573140.1
CDS	510..2540
Translation	MDRSRSSRAASSNQFIRPCGLLTTVILLQTLVSMSLAIEEMSPPPAAPPRPSPPPTVKLNKCCHSGEYLNDGTCIAGSEALWLPMVYLVQQQRFFEPHGASPRFLKFLPNTRPTCRKDQTTEIFRSRGANVMLFPNGTLYVRERALMVQPSDYCVDWEVAVVCLNDSQPINALEDPDYAANPLVQQEPPKASLRLSKCCGKWGSYNTQLQNCDLQPNHQAAVDGLLRLSPQLPEGSYQTSYGLPDCGQPGGYSIAGDWQDAKLDRNTAMLQLPHKNLSAGQYCLEHTQREGEVKIIACQHLFSSAAGAGIHDGSIGGTIEQANGQNLQKAVLTGGILVSIVFLSATLVAGFLLPAVHHALHWRCQICYVTCLLFGKILLAIEELSSSLQPGSAACHTLAITMQFFFLAAFFWLNTMCFNIWWTFRDFRPSSLERNQEALRRYLYSLYAWGGPLLITFVAACVDQLPETTLLRPGFGQLYCWFDNRNLSIFAYFYGPIGLLLCANIALFVSTTHQLTCGLWKRDDVKSSSEKSALGRVCLKLVVVMGVTWIADILSWLVGGPHGVWFFTDLINALQGVFIFIVVGCQPQVWTACRRIFCPRLRHDITNTTNGVQHSSSSQGLPSMAGGTEITQNTTTTTTTTNTTATHMPSNPAEDEVPEKAPIAPVAPIVKMETIC

Target ORF information:

RefSeq Version	NM_132912.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster methuselah-like 1 (mthl1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132912.2

ORF Insert Sequence:

ATGGACAGGA GTCGGAGTAG CAGAGCTGCC AGCAGCAACC AGTTCATCAG GCCATGTGGC 
CTACTGACGA CCGTCATCCT GCTGCAGACG CTTGTGTCGA TGAGTTTGGC CATCGAGGAG 
ATGTCGCCAC CACCGGCCGC CCCGCCACGT CCATCGCCGC CGCCCACCGT GAAGCTCAAC 
AAGTGCTGCC ATTCGGGCGA GTATCTGAAC GATGGCACCT GCATCGCCGG CAGCGAAGCC 
CTGTGGCTGC CCATGGTGTA CTTGGTGCAG CAGCAGCGCT TCTTCGAGCC CCATGGCGCC 
AGTCCGCGAT TCTTGAAGTT CCTGCCCAAT ACGAGACCCA CGTGCCGAAA GGACCAGACG 
ACGGAGATCT TTCGCAGCCG CGGAGCCAAT GTGATGCTCT TTCCCAATGG CACGCTCTAT 
GTGCGGGAAC GCGCTCTAAT GGTCCAGCCG TCGGATTACT GTGTCGACTG GGAGGTGGCC 
GTCGTTTGCT TGAACGACAG TCAGCCGATC AATGCTCTGG AGGATCCCGA TTATGCAGCC 
AATCCTCTCG TGCAGCAGGA GCCGCCCAAG GCTAGCCTGC GACTGAGCAA GTGCTGCGGC 
AAGTGGGGCA GCTATAACAC GCAGCTCCAG AACTGCGATC TGCAGCCCAA CCATCAGGCA 
GCCGTCGATG GACTGCTGCG ATTGTCGCCG CAGCTACCGG AGGGCAGCTA TCAGACTAGC 
TACGGCCTGC CCGACTGTGG CCAACCCGGT GGCTACTCCA TTGCCGGTGA TTGGCAGGAT 
GCCAAGCTGG ATCGGAACAC GGCCATGCTC CAGTTGCCGC ACAAGAACCT CAGCGCCGGA 
CAATACTGCC TGGAGCACAC GCAGCGCGAG GGCGAGGTGA AGATCATAGC CTGCCAGCAT 
TTGTTTAGCT CCGCGGCAGG AGCCGGCATC CACGACGGCT CCATTGGAGG CACCATCGAG 
CAGGCCAATG GCCAGAATCT GCAGAAGGCC GTCCTGACTG GTGGCATACT TGTGTCCATT 
GTCTTCCTGT CCGCCACTTT GGTGGCCGGC TTCCTGCTGC CCGCTGTGCA TCATGCGCTT 
CATTGGCGCT GCCAAATCTG CTATGTCACC TGCTTGCTAT TCGGCAAAAT ACTGCTGGCC 
ATCGAGGAGC TGAGCTCCAG CCTGCAGCCG GGCAGTGCCG CCTGCCATAC GCTTGCTATC 
ACCATGCAGT TCTTCTTTCT GGCCGCCTTC TTTTGGCTGA ACACCATGTG CTTCAACATC 
TGGTGGACGT TCCGGGACTT TCGGCCCAGT TCGTTGGAAC GCAATCAGGA GGCACTGCGT 
CGCTATCTGT ACTCCCTGTA CGCTTGGGGC GGTCCTCTGC TTATCACCTT CGTGGCCGCC 
TGCGTGGACC AGCTGCCGGA GACGACGCTA CTGCGTCCGG GATTCGGACA GCTCTACTGC 
TGGTTCGACA ATCGCAATCT CTCGATCTTC GCCTACTTCT ACGGACCCAT TGGCCTGCTG 
CTGTGCGCCA ATATAGCGCT CTTCGTGTCC ACCACCCATC AGTTGACGTG CGGCCTGTGG 
AAACGGGACG ATGTCAAGTC CTCATCGGAG AAGTCCGCCC TGGGACGCGT CTGCCTGAAG 
CTAGTCGTCG TTATGGGTGT CACCTGGATA GCGGACATTC TGTCCTGGCT AGTGGGTGGA 
CCGCATGGCG TATGGTTCTT CACCGACCTG ATCAACGCCC TGCAAGGCGT CTTCATCTTC 
ATCGTGGTGG GCTGCCAGCC GCAGGTGTGG ACCGCCTGCC GCAGGATCTT CTGTCCGCGA 
TTGCGTCATG ACATCACCAA TACGACCAAC GGTGTCCAGC ATTCGAGCAG CTCGCAGGGT 
TTGCCCTCGA TGGCCGGCGG CACGGAGATT ACACAGAACA CCACCACCAC CACCACGACA 
ACCAACACGA CCGCCACTCA CATGCCCAGC AATCCGGCGG AGGATGAGGT ACCGGAGAAG 
GCTCCCATTG CCCCCGTCGC ACCCATCGTC AAAATGGAGA CCATTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mthl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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