CG42353 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG42353
Entrez Gene ID	32576
Full Name	CG42353 gene product from transcript CG42353-RA
Synonyms	CG9216,Dmel\CG42353,Dmel_CG9216
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14A9-14A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_167485.4	NP_727936.2	uncharacterized protein, isoform A
NM_132878.4	NP_573106.2	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG42353 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG42353 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01757	NM_132878.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG42353), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01757	NM_167485.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG42353), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG42353 ( NM_167485.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727936.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG42353), mRNA.

Price & Availability↑

CloneID	OFa01757
Clone ID Related Accession (Same CDS sequence)	NM_167485.4 , NM_132878.4
Accession Version	NM_167485.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167485.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACA CCATTGCATC CGAAAAAGAA GGAATCTGTC CTCTAAACGA ATCAAAGTCA 
GGTCAGGAAA TTGATACACC AGTTGACCAA GATTCACAGA AATTGGAGCA AGTTATCTTG 
GACAGCTACA TAACAGCACC TCTTAAAATC GTTGAGCCCT TCGAGAAGTT CGAAAAGGAA 
AAGGAGGAGG AAGACAAAGA GAAGGATAGT CCGGTAAGGA GCTCGACAAG TCCGGAGGAT 
CTCACCGAGA TCGAAGTCAT TAGTGCCAAG GCTAAGAAAT CGGTTAGCTT CAATCCCAAC 
GACGAAGAGA TTCGAAAGTT CACCACCGGT GAACCGATTG TCGATCAAAA GAATCCATTC 
CGCAATGGTA ACATAATAGC AAATACGCGT GAGAAGAAGA CACCGCCTCC GGTGCCAACG 
AAACGATCGA CCCTAAGTGT TCGCAAAACC GTGACCCAGC AACTTAGGGA ACGCGAACGA 
GAACGGGAGC GCGAAAAGGA GCGGGAGCAG CAGAAGAACG AGTCCAACAC TCGAAAAAGT 
AAGATTAATC GAACCCAGAC GCAGACGGAT GACTTTGTGA CCACTGAGGA GGTGCTCAAG 
CAATCCAAGT ACGTGAAAAC GTACATCAAG AATCCGGATG CGTACTTTGT CTATGATCCC 
TCGGTGCTGG CACGCCTTAA GTTAGAGGAA CTCAAGGAGA CAACCGGCAA ATTGCCCAAG 
CGGAAACAGA CGTTGAAGGA AACAAGAACG GGAAAACAGG CCCGCCATCA GAATCAGAAT 
CCCAACCAGA ACCAGAAGGC TCAGCAGTTA ATAAAACCGC CGCAGCCCAC TTTTAAGAAG 
TGCTCGAAGC CAAACTATCC GGAGTTGGCC AACTTAAAAA TTCGAACCGG TACAAATTCG 
GATCCAGAAA GCAGTGCCTT ATTCAATCCC GCTGAGGTAA CGAAAAATGC TCGCAAATTC 
GATGAGAGGG TAAAGAAACT ACAGATCACC TCGGATGATG ATCTAGACGA TATCGATGGA 
CCATTGACGA AAAGTGAGTC CAGTGACGAG TTGGCGGCCA GTTCCTCTGT GCCACAGAGT 
CCGGACAAGC CGGTGGCTAT GAGTGCGCCG GGGGATTTAC CCTCGCCATC ACCGGGCACA 
TTCACCAATA CCATTAGTTC CGATGAGTTC CAAGCGTATC TGGACTCGAA GGGTCTGGCC 
CTAATGCCCA GGCGGGAGCT ATTCTCACCG ACACCCACAA TCGCCAGGAC TTCGACGCCA 
ATGCAAACGG CAGAGGAGCG ACGGCAACAG GTGGCTGAAT CACTGGCGGC TCTGCGCAAA 
AGCAACACCA AGAAGCTAAA TGTACTCCAG CGTATCTCAA ACAGTATATT TCCAGCTCGA 
CGAAAAACTA CTCCCAAATC GGAAACTCCT TCGCCACGGA GTATTTTCAA CGAATTGGAA 
AAGGAGGACT ATCGTCGCAA GGCGGATGTC CCAACTGAGA TACGTCGTGT ACTATTGGAA 
CGCCAGAGTC CTAATTGTCG TCGGCAAAAT GGTCAAGTTC AATCGCCGAC CATTGATTCT 
GGTATCGATA AAAGTCCCAG AAATTGTGAA AGCAATTTGC CGAACAGAAA CGGTGATACA 
AGTTTACAAA GGAGAAATGG TGACATCAAT TTGCAAATGC GAAATGGTGA CACCAGTTTG 
CAGATAAGAA ACGGTGACAA CACAATTTTG CAAAGGCGAA ACGGTGACAA CAACTTGCGA 
AGATCAACAC TGGAGCGACC AGGTGTGAAT CCTAGACGTC AGTGGCCAGA AGCACAGCAG 
GTGGGTCGAA TAAATCGATC CACTTCAGTG GATCGCAGCC AATTCAATCA TGAGCAGCCT 
GCTCTTGCAA GTCCTGTTGC CTCATCGACG CCAGTGCGTG GCCAAAATCA ATGGGATCAA 
TTGCGGGCCA TCAAAGAGGT GACGGATCGC CAGCTTTATC ACAAGCTTCA TCAGCAGCAG 
CAACTGCAGG CACAACCACT CCAGGGACAA CAACAACAGC AGCAGCAGCA GCACACAATC 
AGACAACGAC CGAATGAGGA TATCTACTCC CAGGTGATAG TCCATCCCGA CCAAACCAAC 
TTTGTTCGCG GCTCAATGCA GCGTAACACC TTCTCCGGAA TCAGCGGTCG CAGCAGGCCA 
CTAATGGTTT ATAATGGTAC ACCGATGAGG CAACAACAAA TGCAAAAACA GCCGCCGCGA 
TGTCAGAGTG TCCTGGACAA TATAACGCCA AGTGCTGGAA ATCCAGGCCA ACAGACACCA 
GTGGTAATGC GGCATAGGGT GGCGAACCAG GGCCAATCGC AGCAAATGCC ATCACGTCGC 
ATCGGTGGAC TACTCACGCG CGAGGAGATT CTCGAAAAGG TCAAGGAGTT TTGCCGCAAG 
AGCATTAACC GAGCACCGGA AGCCAAGACG CCACTGCAGC CCATCTATAA GCGACATCTA 
ACGCCGCCCA ACGCCGATGT CTCGCCCGTG TCATACGCTT CTGTCGATGA TCAATGTGCT 
CGACCACAGG TGCCACAGCG TGTCCAGAGT TTACCTGTCG CCCAGGGGCG CTACGTCACC 
AGCGGCATGT CAGTGGACGG GCCGTCACCC ATTTACGCTC ATGTCAGCAA GCGAAACAGT 
TTGCTTTCCA ATGTCTCCGA ACAGTATTTA TCCAACCAGC AACTTGGAAC ACTGACCAGG 
CCGGTGCCGC TGAACCAGTT GGTACTGGTG GATACGGGCG AGGGTGTTCA GGTGGCCCAG 
CTAGTAGACT ATCTACCCTA TGTCCGACCG GCTCCCGCCC AGGGCTATAT GATGCAGGAT 
GGCAGGGCCA CTCCACTCAT CCTGGACCAA TCGGCCCAGC AGAGCAGCCA AATTTACTGG 
ACACCGCAGC ATCGTCAGCG TTTCTCTCAT CCGGTGCCGG CACCCCGTTT TATGAAGAGT 
CAAGCCATGC CCACATCAAG AAACAGCACC ATAAGTCGAC ATATGGAGGA AAGGATGGGT 
AACGCCTCCG ACTGGGAACT CAGCTCGGAA GCTGGTGAAG TGAGGCGTAT AATGGAAAAA 
CAATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727936.2
CDS	275..3343
Translation	MSDTIASEKEGICPLNESKSGQEIDTPVDQDSQKLEQVILDSYITAPLKIVEPFEKFEKEKEEEDKEKDSPVRSSTSPEDLTEIEVISAKAKKSVSFNPNDEEIRKFTTGEPIVDQKNPFRNGNIIANTREKKTPPPVPTKRSTLSVRKTVTQQLREREREREREKEREQQKNESNTRKSKINRTQTQTDDFVTTEEVLKQSKYVKTYIKNPDAYFVYDPSVLARLKLEELKETTGKLPKRKQTLKETRTGKQARHQNQNPNQNQKAQQLIKPPQPTFKKCSKPNYPELANLKIRTGTNSDPESSALFNPAEVTKNARKFDERVKKLQITSDDDLDDIDGPLTKSESSDELAASSSVPQSPDKPVAMSAPGDLPSPSPGTFTNTISSDEFQAYLDSKGLALMPRRELFSPTPTIARTSTPMQTAEERRQQVAESLAALRKSNTKKLNVLQRISNSIFPARRKTTPKSETPSPRSIFNELEKEDYRRKADVPTEIRRVLLERQSPNCRRQNGQVQSPTIDSGIDKSPRNCESNLPNRNGDTSLQRRNGDINLQMRNGDTSLQIRNGDNTILQRRNGDNNLRRSTLERPGVNPRRQWPEAQQVGRINRSTSVDRSQFNHEQPALASPVASSTPVRGQNQWDQLRAIKEVTDRQLYHKLHQQQQLQAQPLQGQQQQQQQQHTIRQRPNEDIYSQVIVHPDQTNFVRGSMQRNTFSGISGRSRPLMVYNGTPMRQQQMQKQPPRCQSVLDNITPSAGNPGQQTPVVMRHRVANQGQSQQMPSRRIGGLLTREEILEKVKEFCRKSINRAPEAKTPLQPIYKRHLTPPNADVSPVSYASVDDQCARPQVPQRVQSLPVAQGRYVTSGMSVDGPSPIYAHVSKRNSLLSNVSEQYLSNQQLGTLTRPVPLNQLVLVDTGEGVQVAQLVDYLPYVRPAPAQGYMMQDGRATPLILDQSAQQSSQIYWTPQHRQRFSHPVPAPRFMKSQAMPTSRNSTISRHMEERMGNASDWELSSEAGEVRRIMEKQL

Target ORF information:

RefSeq Version	NM_167485.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG42353), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167485.4

ORF Insert Sequence:

ATGTCTGACA CCATTGCATC CGAAAAAGAA GGAATCTGTC CTCTAAACGA ATCAAAGTCA 
GGTCAGGAAA TTGATACACC AGTTGACCAA GATTCACAGA AATTGGAGCA AGTTATCTTG 
GACAGCTACA TAACAGCACC TCTTAAAATC GTTGAGCCCT TCGAGAAGTT CGAAAAGGAA 
AAGGAGGAGG AAGACAAAGA GAAGGATAGT CCGGTAAGGA GCTCGACAAG TCCGGAGGAT 
CTCACCGAGA TCGAAGTCAT TAGTGCCAAG GCTAAGAAAT CGGTTAGCTT CAATCCCAAC 
GACGAAGAGA TTCGAAAGTT CACCACCGGT GAACCGATTG TCGATCAAAA GAATCCATTC 
CGCAATGGTA ACATAATAGC AAATACGCGT GAGAAGAAGA CACCGCCTCC GGTGCCAACG 
AAACGATCGA CCCTAAGTGT TCGCAAAACC GTGACCCAGC AACTTAGGGA ACGCGAACGA 
GAACGGGAGC GCGAAAAGGA GCGGGAGCAG CAGAAGAACG AGTCCAACAC TCGAAAAAGT 
AAGATTAATC GAACCCAGAC GCAGACGGAT GACTTTGTGA CCACTGAGGA GGTGCTCAAG 
CAATCCAAGT ACGTGAAAAC GTACATCAAG AATCCGGATG CGTACTTTGT CTATGATCCC 
TCGGTGCTGG CACGCCTTAA GTTAGAGGAA CTCAAGGAGA CAACCGGCAA ATTGCCCAAG 
CGGAAACAGA CGTTGAAGGA AACAAGAACG GGAAAACAGG CCCGCCATCA GAATCAGAAT 
CCCAACCAGA ACCAGAAGGC TCAGCAGTTA ATAAAACCGC CGCAGCCCAC TTTTAAGAAG 
TGCTCGAAGC CAAACTATCC GGAGTTGGCC AACTTAAAAA TTCGAACCGG TACAAATTCG 
GATCCAGAAA GCAGTGCCTT ATTCAATCCC GCTGAGGTAA CGAAAAATGC TCGCAAATTC 
GATGAGAGGG TAAAGAAACT ACAGATCACC TCGGATGATG ATCTAGACGA TATCGATGGA 
CCATTGACGA AAAGTGAGTC CAGTGACGAG TTGGCGGCCA GTTCCTCTGT GCCACAGAGT 
CCGGACAAGC CGGTGGCTAT GAGTGCGCCG GGGGATTTAC CCTCGCCATC ACCGGGCACA 
TTCACCAATA CCATTAGTTC CGATGAGTTC CAAGCGTATC TGGACTCGAA GGGTCTGGCC 
CTAATGCCCA GGCGGGAGCT ATTCTCACCG ACACCCACAA TCGCCAGGAC TTCGACGCCA 
ATGCAAACGG CAGAGGAGCG ACGGCAACAG GTGGCTGAAT CACTGGCGGC TCTGCGCAAA 
AGCAACACCA AGAAGCTAAA TGTACTCCAG CGTATCTCAA ACAGTATATT TCCAGCTCGA 
CGAAAAACTA CTCCCAAATC GGAAACTCCT TCGCCACGGA GTATTTTCAA CGAATTGGAA 
AAGGAGGACT ATCGTCGCAA GGCGGATGTC CCAACTGAGA TACGTCGTGT ACTATTGGAA 
CGCCAGAGTC CTAATTGTCG TCGGCAAAAT GGTCAAGTTC AATCGCCGAC CATTGATTCT 
GGTATCGATA AAAGTCCCAG AAATTGTGAA AGCAATTTGC CGAACAGAAA CGGTGATACA 
AGTTTACAAA GGAGAAATGG TGACATCAAT TTGCAAATGC GAAATGGTGA CACCAGTTTG 
CAGATAAGAA ACGGTGACAA CACAATTTTG CAAAGGCGAA ACGGTGACAA CAACTTGCGA 
AGATCAACAC TGGAGCGACC AGGTGTGAAT CCTAGACGTC AGTGGCCAGA AGCACAGCAG 
GTGGGTCGAA TAAATCGATC CACTTCAGTG GATCGCAGCC AATTCAATCA TGAGCAGCCT 
GCTCTTGCAA GTCCTGTTGC CTCATCGACG CCAGTGCGTG GCCAAAATCA ATGGGATCAA 
TTGCGGGCCA TCAAAGAGGT GACGGATCGC CAGCTTTATC ACAAGCTTCA TCAGCAGCAG 
CAACTGCAGG CACAACCACT CCAGGGACAA CAACAACAGC AGCAGCAGCA GCACACAATC 
AGACAACGAC CGAATGAGGA TATCTACTCC CAGGTGATAG TCCATCCCGA CCAAACCAAC 
TTTGTTCGCG GCTCAATGCA GCGTAACACC TTCTCCGGAA TCAGCGGTCG CAGCAGGCCA 
CTAATGGTTT ATAATGGTAC ACCGATGAGG CAACAACAAA TGCAAAAACA GCCGCCGCGA 
TGTCAGAGTG TCCTGGACAA TATAACGCCA AGTGCTGGAA ATCCAGGCCA ACAGACACCA 
GTGGTAATGC GGCATAGGGT GGCGAACCAG GGCCAATCGC AGCAAATGCC ATCACGTCGC 
ATCGGTGGAC TACTCACGCG CGAGGAGATT CTCGAAAAGG TCAAGGAGTT TTGCCGCAAG 
AGCATTAACC GAGCACCGGA AGCCAAGACG CCACTGCAGC CCATCTATAA GCGACATCTA 
ACGCCGCCCA ACGCCGATGT CTCGCCCGTG TCATACGCTT CTGTCGATGA TCAATGTGCT 
CGACCACAGG TGCCACAGCG TGTCCAGAGT TTACCTGTCG CCCAGGGGCG CTACGTCACC 
AGCGGCATGT CAGTGGACGG GCCGTCACCC ATTTACGCTC ATGTCAGCAA GCGAAACAGT 
TTGCTTTCCA ATGTCTCCGA ACAGTATTTA TCCAACCAGC AACTTGGAAC ACTGACCAGG 
CCGGTGCCGC TGAACCAGTT GGTACTGGTG GATACGGGCG AGGGTGTTCA GGTGGCCCAG 
CTAGTAGACT ATCTACCCTA TGTCCGACCG GCTCCCGCCC AGGGCTATAT GATGCAGGAT 
GGCAGGGCCA CTCCACTCAT CCTGGACCAA TCGGCCCAGC AGAGCAGCCA AATTTACTGG 
ACACCGCAGC ATCGTCAGCG TTTCTCTCAT CCGGTGCCGG CACCCCGTTT TATGAAGAGT 
CAAGCCATGC CCACATCAAG AAACAGCACC ATAAGTCGAC ATATGGAGGA AAGGATGGGT 
AACGCCTCCG ACTGGGAACT CAGCTCGGAA GCTGGTGAAG TGAGGCGTAT AATGGAAAAA 
CAATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG42353 ( NM_132878.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573106.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG42353), mRNA.

Price & Availability↑

CloneID	OFa01757
Clone ID Related Accession (Same CDS sequence)	NM_167485.4 , NM_132878.4
Accession Version	NM_132878.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132878.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACA CCATTGCATC CGAAAAAGAA GGAATCTGTC CTCTAAACGA ATCAAAGTCA 
GGTCAGGAAA TTGATACACC AGTTGACCAA GATTCACAGA AATTGGAGCA AGTTATCTTG 
GACAGCTACA TAACAGCACC TCTTAAAATC GTTGAGCCCT TCGAGAAGTT CGAAAAGGAA 
AAGGAGGAGG AAGACAAAGA GAAGGATAGT CCGGTAAGGA GCTCGACAAG TCCGGAGGAT 
CTCACCGAGA TCGAAGTCAT TAGTGCCAAG GCTAAGAAAT CGGTTAGCTT CAATCCCAAC 
GACGAAGAGA TTCGAAAGTT CACCACCGGT GAACCGATTG TCGATCAAAA GAATCCATTC 
CGCAATGGTA ACATAATAGC AAATACGCGT GAGAAGAAGA CACCGCCTCC GGTGCCAACG 
AAACGATCGA CCCTAAGTGT TCGCAAAACC GTGACCCAGC AACTTAGGGA ACGCGAACGA 
GAACGGGAGC GCGAAAAGGA GCGGGAGCAG CAGAAGAACG AGTCCAACAC TCGAAAAAGT 
AAGATTAATC GAACCCAGAC GCAGACGGAT GACTTTGTGA CCACTGAGGA GGTGCTCAAG 
CAATCCAAGT ACGTGAAAAC GTACATCAAG AATCCGGATG CGTACTTTGT CTATGATCCC 
TCGGTGCTGG CACGCCTTAA GTTAGAGGAA CTCAAGGAGA CAACCGGCAA ATTGCCCAAG 
CGGAAACAGA CGTTGAAGGA AACAAGAACG GGAAAACAGG CCCGCCATCA GAATCAGAAT 
CCCAACCAGA ACCAGAAGGC TCAGCAGTTA ATAAAACCGC CGCAGCCCAC TTTTAAGAAG 
TGCTCGAAGC CAAACTATCC GGAGTTGGCC AACTTAAAAA TTCGAACCGG TACAAATTCG 
GATCCAGAAA GCAGTGCCTT ATTCAATCCC GCTGAGGTAA CGAAAAATGC TCGCAAATTC 
GATGAGAGGG TAAAGAAACT ACAGATCACC TCGGATGATG ATCTAGACGA TATCGATGGA 
CCATTGACGA AAAGTGAGTC CAGTGACGAG TTGGCGGCCA GTTCCTCTGT GCCACAGAGT 
CCGGACAAGC CGGTGGCTAT GAGTGCGCCG GGGGATTTAC CCTCGCCATC ACCGGGCACA 
TTCACCAATA CCATTAGTTC CGATGAGTTC CAAGCGTATC TGGACTCGAA GGGTCTGGCC 
CTAATGCCCA GGCGGGAGCT ATTCTCACCG ACACCCACAA TCGCCAGGAC TTCGACGCCA 
ATGCAAACGG CAGAGGAGCG ACGGCAACAG GTGGCTGAAT CACTGGCGGC TCTGCGCAAA 
AGCAACACCA AGAAGCTAAA TGTACTCCAG CGTATCTCAA ACAGTATATT TCCAGCTCGA 
CGAAAAACTA CTCCCAAATC GGAAACTCCT TCGCCACGGA GTATTTTCAA CGAATTGGAA 
AAGGAGGACT ATCGTCGCAA GGCGGATGTC CCAACTGAGA TACGTCGTGT ACTATTGGAA 
CGCCAGAGTC CTAATTGTCG TCGGCAAAAT GGTCAAGTTC AATCGCCGAC CATTGATTCT 
GGTATCGATA AAAGTCCCAG AAATTGTGAA AGCAATTTGC CGAACAGAAA CGGTGATACA 
AGTTTACAAA GGAGAAATGG TGACATCAAT TTGCAAATGC GAAATGGTGA CACCAGTTTG 
CAGATAAGAA ACGGTGACAA CACAATTTTG CAAAGGCGAA ACGGTGACAA CAACTTGCGA 
AGATCAACAC TGGAGCGACC AGGTGTGAAT CCTAGACGTC AGTGGCCAGA AGCACAGCAG 
GTGGGTCGAA TAAATCGATC CACTTCAGTG GATCGCAGCC AATTCAATCA TGAGCAGCCT 
GCTCTTGCAA GTCCTGTTGC CTCATCGACG CCAGTGCGTG GCCAAAATCA ATGGGATCAA 
TTGCGGGCCA TCAAAGAGGT GACGGATCGC CAGCTTTATC ACAAGCTTCA TCAGCAGCAG 
CAACTGCAGG CACAACCACT CCAGGGACAA CAACAACAGC AGCAGCAGCA GCACACAATC 
AGACAACGAC CGAATGAGGA TATCTACTCC CAGGTGATAG TCCATCCCGA CCAAACCAAC 
TTTGTTCGCG GCTCAATGCA GCGTAACACC TTCTCCGGAA TCAGCGGTCG CAGCAGGCCA 
CTAATGGTTT ATAATGGTAC ACCGATGAGG CAACAACAAA TGCAAAAACA GCCGCCGCGA 
TGTCAGAGTG TCCTGGACAA TATAACGCCA AGTGCTGGAA ATCCAGGCCA ACAGACACCA 
GTGGTAATGC GGCATAGGGT GGCGAACCAG GGCCAATCGC AGCAAATGCC ATCACGTCGC 
ATCGGTGGAC TACTCACGCG CGAGGAGATT CTCGAAAAGG TCAAGGAGTT TTGCCGCAAG 
AGCATTAACC GAGCACCGGA AGCCAAGACG CCACTGCAGC CCATCTATAA GCGACATCTA 
ACGCCGCCCA ACGCCGATGT CTCGCCCGTG TCATACGCTT CTGTCGATGA TCAATGTGCT 
CGACCACAGG TGCCACAGCG TGTCCAGAGT TTACCTGTCG CCCAGGGGCG CTACGTCACC 
AGCGGCATGT CAGTGGACGG GCCGTCACCC ATTTACGCTC ATGTCAGCAA GCGAAACAGT 
TTGCTTTCCA ATGTCTCCGA ACAGTATTTA TCCAACCAGC AACTTGGAAC ACTGACCAGG 
CCGGTGCCGC TGAACCAGTT GGTACTGGTG GATACGGGCG AGGGTGTTCA GGTGGCCCAG 
CTAGTAGACT ATCTACCCTA TGTCCGACCG GCTCCCGCCC AGGGCTATAT GATGCAGGAT 
GGCAGGGCCA CTCCACTCAT CCTGGACCAA TCGGCCCAGC AGAGCAGCCA AATTTACTGG 
ACACCGCAGC ATCGTCAGCG TTTCTCTCAT CCGGTGCCGG CACCCCGTTT TATGAAGAGT 
CAAGCCATGC CCACATCAAG AAACAGCACC ATAAGTCGAC ATATGGAGGA AAGGATGGGT 
AACGCCTCCG ACTGGGAACT CAGCTCGGAA GCTGGTGAAG TGAGGCGTAT AATGGAAAAA 
CAATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573106.2
CDS	510..3578
Translation	MSDTIASEKEGICPLNESKSGQEIDTPVDQDSQKLEQVILDSYITAPLKIVEPFEKFEKEKEEEDKEKDSPVRSSTSPEDLTEIEVISAKAKKSVSFNPNDEEIRKFTTGEPIVDQKNPFRNGNIIANTREKKTPPPVPTKRSTLSVRKTVTQQLREREREREREKEREQQKNESNTRKSKINRTQTQTDDFVTTEEVLKQSKYVKTYIKNPDAYFVYDPSVLARLKLEELKETTGKLPKRKQTLKETRTGKQARHQNQNPNQNQKAQQLIKPPQPTFKKCSKPNYPELANLKIRTGTNSDPESSALFNPAEVTKNARKFDERVKKLQITSDDDLDDIDGPLTKSESSDELAASSSVPQSPDKPVAMSAPGDLPSPSPGTFTNTISSDEFQAYLDSKGLALMPRRELFSPTPTIARTSTPMQTAEERRQQVAESLAALRKSNTKKLNVLQRISNSIFPARRKTTPKSETPSPRSIFNELEKEDYRRKADVPTEIRRVLLERQSPNCRRQNGQVQSPTIDSGIDKSPRNCESNLPNRNGDTSLQRRNGDINLQMRNGDTSLQIRNGDNTILQRRNGDNNLRRSTLERPGVNPRRQWPEAQQVGRINRSTSVDRSQFNHEQPALASPVASSTPVRGQNQWDQLRAIKEVTDRQLYHKLHQQQQLQAQPLQGQQQQQQQQHTIRQRPNEDIYSQVIVHPDQTNFVRGSMQRNTFSGISGRSRPLMVYNGTPMRQQQMQKQPPRCQSVLDNITPSAGNPGQQTPVVMRHRVANQGQSQQMPSRRIGGLLTREEILEKVKEFCRKSINRAPEAKTPLQPIYKRHLTPPNADVSPVSYASVDDQCARPQVPQRVQSLPVAQGRYVTSGMSVDGPSPIYAHVSKRNSLLSNVSEQYLSNQQLGTLTRPVPLNQLVLVDTGEGVQVAQLVDYLPYVRPAPAQGYMMQDGRATPLILDQSAQQSSQIYWTPQHRQRFSHPVPAPRFMKSQAMPTSRNSTISRHMEERMGNASDWELSSEAGEVRRIMEKQL

Target ORF information:

RefSeq Version	NM_132878.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG42353), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132878.4

ORF Insert Sequence:

ATGTCTGACA CCATTGCATC CGAAAAAGAA GGAATCTGTC CTCTAAACGA ATCAAAGTCA 
GGTCAGGAAA TTGATACACC AGTTGACCAA GATTCACAGA AATTGGAGCA AGTTATCTTG 
GACAGCTACA TAACAGCACC TCTTAAAATC GTTGAGCCCT TCGAGAAGTT CGAAAAGGAA 
AAGGAGGAGG AAGACAAAGA GAAGGATAGT CCGGTAAGGA GCTCGACAAG TCCGGAGGAT 
CTCACCGAGA TCGAAGTCAT TAGTGCCAAG GCTAAGAAAT CGGTTAGCTT CAATCCCAAC 
GACGAAGAGA TTCGAAAGTT CACCACCGGT GAACCGATTG TCGATCAAAA GAATCCATTC 
CGCAATGGTA ACATAATAGC AAATACGCGT GAGAAGAAGA CACCGCCTCC GGTGCCAACG 
AAACGATCGA CCCTAAGTGT TCGCAAAACC GTGACCCAGC AACTTAGGGA ACGCGAACGA 
GAACGGGAGC GCGAAAAGGA GCGGGAGCAG CAGAAGAACG AGTCCAACAC TCGAAAAAGT 
AAGATTAATC GAACCCAGAC GCAGACGGAT GACTTTGTGA CCACTGAGGA GGTGCTCAAG 
CAATCCAAGT ACGTGAAAAC GTACATCAAG AATCCGGATG CGTACTTTGT CTATGATCCC 
TCGGTGCTGG CACGCCTTAA GTTAGAGGAA CTCAAGGAGA CAACCGGCAA ATTGCCCAAG 
CGGAAACAGA CGTTGAAGGA AACAAGAACG GGAAAACAGG CCCGCCATCA GAATCAGAAT 
CCCAACCAGA ACCAGAAGGC TCAGCAGTTA ATAAAACCGC CGCAGCCCAC TTTTAAGAAG 
TGCTCGAAGC CAAACTATCC GGAGTTGGCC AACTTAAAAA TTCGAACCGG TACAAATTCG 
GATCCAGAAA GCAGTGCCTT ATTCAATCCC GCTGAGGTAA CGAAAAATGC TCGCAAATTC 
GATGAGAGGG TAAAGAAACT ACAGATCACC TCGGATGATG ATCTAGACGA TATCGATGGA 
CCATTGACGA AAAGTGAGTC CAGTGACGAG TTGGCGGCCA GTTCCTCTGT GCCACAGAGT 
CCGGACAAGC CGGTGGCTAT GAGTGCGCCG GGGGATTTAC CCTCGCCATC ACCGGGCACA 
TTCACCAATA CCATTAGTTC CGATGAGTTC CAAGCGTATC TGGACTCGAA GGGTCTGGCC 
CTAATGCCCA GGCGGGAGCT ATTCTCACCG ACACCCACAA TCGCCAGGAC TTCGACGCCA 
ATGCAAACGG CAGAGGAGCG ACGGCAACAG GTGGCTGAAT CACTGGCGGC TCTGCGCAAA 
AGCAACACCA AGAAGCTAAA TGTACTCCAG CGTATCTCAA ACAGTATATT TCCAGCTCGA 
CGAAAAACTA CTCCCAAATC GGAAACTCCT TCGCCACGGA GTATTTTCAA CGAATTGGAA 
AAGGAGGACT ATCGTCGCAA GGCGGATGTC CCAACTGAGA TACGTCGTGT ACTATTGGAA 
CGCCAGAGTC CTAATTGTCG TCGGCAAAAT GGTCAAGTTC AATCGCCGAC CATTGATTCT 
GGTATCGATA AAAGTCCCAG AAATTGTGAA AGCAATTTGC CGAACAGAAA CGGTGATACA 
AGTTTACAAA GGAGAAATGG TGACATCAAT TTGCAAATGC GAAATGGTGA CACCAGTTTG 
CAGATAAGAA ACGGTGACAA CACAATTTTG CAAAGGCGAA ACGGTGACAA CAACTTGCGA 
AGATCAACAC TGGAGCGACC AGGTGTGAAT CCTAGACGTC AGTGGCCAGA AGCACAGCAG 
GTGGGTCGAA TAAATCGATC CACTTCAGTG GATCGCAGCC AATTCAATCA TGAGCAGCCT 
GCTCTTGCAA GTCCTGTTGC CTCATCGACG CCAGTGCGTG GCCAAAATCA ATGGGATCAA 
TTGCGGGCCA TCAAAGAGGT GACGGATCGC CAGCTTTATC ACAAGCTTCA TCAGCAGCAG 
CAACTGCAGG CACAACCACT CCAGGGACAA CAACAACAGC AGCAGCAGCA GCACACAATC 
AGACAACGAC CGAATGAGGA TATCTACTCC CAGGTGATAG TCCATCCCGA CCAAACCAAC 
TTTGTTCGCG GCTCAATGCA GCGTAACACC TTCTCCGGAA TCAGCGGTCG CAGCAGGCCA 
CTAATGGTTT ATAATGGTAC ACCGATGAGG CAACAACAAA TGCAAAAACA GCCGCCGCGA 
TGTCAGAGTG TCCTGGACAA TATAACGCCA AGTGCTGGAA ATCCAGGCCA ACAGACACCA 
GTGGTAATGC GGCATAGGGT GGCGAACCAG GGCCAATCGC AGCAAATGCC ATCACGTCGC 
ATCGGTGGAC TACTCACGCG CGAGGAGATT CTCGAAAAGG TCAAGGAGTT TTGCCGCAAG 
AGCATTAACC GAGCACCGGA AGCCAAGACG CCACTGCAGC CCATCTATAA GCGACATCTA 
ACGCCGCCCA ACGCCGATGT CTCGCCCGTG TCATACGCTT CTGTCGATGA TCAATGTGCT 
CGACCACAGG TGCCACAGCG TGTCCAGAGT TTACCTGTCG CCCAGGGGCG CTACGTCACC 
AGCGGCATGT CAGTGGACGG GCCGTCACCC ATTTACGCTC ATGTCAGCAA GCGAAACAGT 
TTGCTTTCCA ATGTCTCCGA ACAGTATTTA TCCAACCAGC AACTTGGAAC ACTGACCAGG 
CCGGTGCCGC TGAACCAGTT GGTACTGGTG GATACGGGCG AGGGTGTTCA GGTGGCCCAG 
CTAGTAGACT ATCTACCCTA TGTCCGACCG GCTCCCGCCC AGGGCTATAT GATGCAGGAT 
GGCAGGGCCA CTCCACTCAT CCTGGACCAA TCGGCCCAGC AGAGCAGCCA AATTTACTGG 
ACACCGCAGC ATCGTCAGCG TTTCTCTCAT CCGGTGCCGG CACCCCGTTT TATGAAGAGT 
CAAGCCATGC CCACATCAAG AAACAGCACC ATAAGTCGAC ATATGGAGGA AAGGATGGGT 
AACGCCTCCG ACTGGGAACT CAGCTCGGAA GCTGGTGAAG TGAGGCGTAT AATGGAAAAA 
CAATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG42353 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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