mh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mh
Entrez Gene ID	32480
Full Name	maternal haploid
Synonyms	CG9203,Dmel\CG9203,fs(1)1182,fs(1)A1182
General protein information	Preferred Names maternal haploid Names CG9203 gene product from transcript CG9203-RA CG9203-PA fs(1)maternal haploid mh-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 13C4-13C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_132804.2	NP_573032.1	maternal haploid

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004082	XP_309634.5
Caenorhabditis elegans (roundworm)	T19B10.6	NP_505853.1
Drosophila melanogaster (fruit fly)	CG9203	NP_573032.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MH is essential for genome integrity during embryogenesis. MH regulates Top2, possibly as a protease for the resolution of Top2-DNA intermediates.
Title: Maternal Haploid, a Metalloprotease Enriched at the Largest Satellite Repeat and Essential for Genome Integrity in

The following mh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01694	NM_132804.2 Latest version!	Drosophila melanogaster maternal haploid (mh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mh ( NM_132804.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_573032.1 Drosophila melanogaster maternal haploid (mh), mRNA.

Price & Availability↑

CloneID	OFa01694
Clone ID Related Accession (Same CDS sequence)	NM_132804.2
Accession Version	NM_132804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maternal haploid
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132804.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGATG CAGACTACTT GTTCGCTCTG AACCTGCAGA ACCAGCTAAA TGCGGAGTCA 
GACCAAAGTT ACACAGCCGC GGATGCCAGC AATGACAGGG ACTATCAGCT GGCACGCAGG 
ATTCAGAACC TAATAAACGA GGGCGATGAG GACGGTGCTA GCGAGCTGGA CAGCAGCATT 
CAGTTCGTCT ACCCCAAATC GAATCAGCCA AAATCAACAA AAATGGAGAA CTCTGAAATC 
AAAAACAAAA AACGAGAAGC GGCTAAAAAG AAGCCAGTGC GCGCTATTCA GGCCAGCCAT 
GATGACTATC TGAATAAGAC GATGAATCTG GTGGATCCGG AGTGGGAGCT GGTCGATCCC 
ACCCCGGACA TCTACGCCAT GTTCATACGC TTCGACGAGA AGTTCTTCCA GGAGCGCCTG 
GGCGCCGTCT CCCTGGAATG GAGCAAGAAG ATGTACTCGT GCGCTGGAAT CTGCTACCAG 
CGTGGCAATC GCTTCGTCAA ACAGGTGACC ATTCGGCTCA GTGAGCCGCT GCTAAAGTTG 
CGTCCGCGCA AGGATCTCGT GGAAACGCTC TTGCACGAAA TGATCCATGC CTACTGCTTT 
GTGCTCAATA TACGCGAGGG TAACGGTGGC CATGGACCCA ATTTCAAGCG CATCATGGAG 
ACGATCAATA AGGTGGCGGG CACCAACATC ACCGTTTACC ACACTTTTCA CGATGAGGTG 
GCCTCCTATC GCACCCACAT CTGGCGCTGC ACGGGCATTT GCCGAGAGCG CTCACCCTTT 
TGGGGCTATG TGAAGCGGAC CAGCAACCGA GCGCCCGGTC CGAACGATCA GTGGTGGAAG 
ATGCATCAAC GTGAGTGCAG CGGCACCTTT TTGAAGTTGA GTGAACCCCC GAAGGCCCCC 
AAGCCGGAGA CCAAGTCAAG GGCTAAGAAG TCCACACCGG CGGTGGCCGC GCCCAAGGAA 
GACATACGCA ACTGGTTCGG CCAGCCGTCA GCCAAGAAAG CGGCCACCGG CACAGTCTTA 
CTAGGCGATC TGGCGAAGGC GGTGCCGCCG ACTTCTTATC CCGGACTCTC TTCCGAACCC 
TTTGCATTGC CCAAGACACC AGCTGCTGGC ACAAAAGGCA ACAAAAGCGG CGGATTCAAT 
GAACTGGACA AATCAGGTTC GAAAGCCACC ACTGAAATGA GCGGACAGGG CTACAGTCTT 
GGTGGCTCTA GCGCTATAGA ACACATAGAT CTTAGTGACA CTGATGTTCC CGATTCCGCT 
AAACTTCGTC AAATTCGACT TCAGCGTTTT AGCGAAGATG CTTCCAACAA GCGACAGAAT 
AAGAAAAAGA GGCGTCGTGT TTCCAGCGAC AGCGAGATAA TCAACTGGGA GTCCTACGAT 
GATGACGTAA TGGTCAGGGA TGTTCATACG CCCATCGTCA ACTTGGTCGA AAGCTCTAGT 
GATGAAGAGG AGATGAAGAT GCCAGCTGGC AGAAAAACCA TGACCAGTCA GGAGCGCACG 
CAGATCATCA AGCGCCAGGT AATGGAGGAT GAGTTGACCT TGTGTGAGGA CGATATCCTC 
TTAATTGATG ATGAATACGA CGATGAGGTG GCCGCAAATG AATCTTTAAC AGCCGCCACG 
GAGCTAGCTG ACCAATCCAT TATAGATGAT ATTTTTGGAG AGGACACCCT GCTGAAGGAG 
TTCCAGCGTG AGAACGACGT GCAGCCCAGT TGCTCAAGCT ACGCCAAAAG AATTGAGAAC 
GACATCGTAT CTTGTCCCAT TTGCTTTGAG AAAATGAGGC GCACTGAGTT GTCCAATCAC 
TTCGATGGCT GCGCCATTAT GGTGCGCTTG GAACCGCCAT CGTTTAAGCC TAAAAACCGC 
CGAACAACTG CTTCCTTAAC CTCAAAGGAC TCGTCGGGAG CTTCCACATC ACGCCGCGCT 
AAATCGAAAG CAAAGTCATC AAAAGGAATT CTTCGCAGCT CCGGTTACAC AGAAGGGGAG 
ATCGACAAGC TCAACCTGAG CTCCTCTACC GACTCGTCTA CTCTGCCGTC TAGCGAGGAC 
GAACTTACTC CGCGTCAGCG CCGCCAGCGC AACCTCTTCA AAGAGACCGT AGGCTGCCCC 
AGATGTGGCC TGGAGATCAT GCCTCTACAC CTGAAGGTCC ATGGATCCGT CTGCGCTGGT 
CGCAAGAAGC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_573032.1
CDS	85..2259
Translation	MSDADYLFALNLQNQLNAESDQSYTAADASNDRDYQLARRIQNLINEGDEDGASELDSSIQFVYPKSNQPKSTKMENSEIKNKKREAAKKKPVRAIQASHDDYLNKTMNLVDPEWELVDPTPDIYAMFIRFDEKFFQERLGAVSLEWSKKMYSCAGICYQRGNRFVKQVTIRLSEPLLKLRPRKDLVETLLHEMIHAYCFVLNIREGNGGHGPNFKRIMETINKVAGTNITVYHTFHDEVASYRTHIWRCTGICRERSPFWGYVKRTSNRAPGPNDQWWKMHQRECSGTFLKLSEPPKAPKPETKSRAKKSTPAVAAPKEDIRNWFGQPSAKKAATGTVLLGDLAKAVPPTSYPGLSSEPFALPKTPAAGTKGNKSGGFNELDKSGSKATTEMSGQGYSLGGSSAIEHIDLSDTDVPDSAKLRQIRLQRFSEDASNKRQNKKKRRRVSSDSEIINWESYDDDVMVRDVHTPIVNLVESSSDEEEMKMPAGRKTMTSQERTQIIKRQVMEDELTLCEDDILLIDDEYDDEVAANESLTAATELADQSIIDDIFGEDTLLKEFQRENDVQPSCSSYAKRIENDIVSCPICFEKMRRTELSNHFDGCAIMVRLEPPSFKPKNRRTTASLTSKDSSGASTSRRAKSKAKSSKGILRSSGYTEGEIDKLNLSSSTDSSTLPSSEDELTPRQRRQRNLFKETVGCPRCGLEIMPLHLKVHGSVCAGRKKR

Target ORF information:

RefSeq Version	NM_132804.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maternal haploid (mh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132804.2

ORF Insert Sequence:

ATGTCAGATG CAGACTACTT GTTCGCTCTG AACCTGCAGA ACCAGCTAAA TGCGGAGTCA 
GACCAAAGTT ACACAGCCGC GGATGCCAGC AATGACAGGG ACTATCAGCT GGCACGCAGG 
ATTCAGAACC TAATAAACGA GGGCGATGAG GACGGTGCTA GCGAGCTGGA CAGCAGCATT 
CAGTTCGTCT ACCCCAAATC GAATCAGCCA AAATCAACAA AAATGGAGAA CTCTGAAATC 
AAAAACAAAA AACGAGAAGC GGCTAAAAAG AAGCCAGTGC GCGCTATTCA GGCCAGCCAT 
GATGACTATC TGAATAAGAC GATGAATCTG GTGGATCCGG AGTGGGAGCT GGTCGATCCC 
ACCCCGGACA TCTACGCCAT GTTCATACGC TTCGACGAGA AGTTCTTCCA GGAGCGCCTG 
GGCGCCGTCT CCCTGGAATG GAGCAAGAAG ATGTACTCGT GCGCTGGAAT CTGCTACCAG 
CGTGGCAATC GCTTCGTCAA ACAGGTGACC ATTCGGCTCA GTGAGCCGCT GCTAAAGTTG 
CGTCCGCGCA AGGATCTCGT GGAAACGCTC TTGCACGAAA TGATCCATGC CTACTGCTTT 
GTGCTCAATA TACGCGAGGG TAACGGTGGC CATGGACCCA ATTTCAAGCG CATCATGGAG 
ACGATCAATA AGGTGGCGGG CACCAACATC ACCGTTTACC ACACTTTTCA CGATGAGGTG 
GCCTCCTATC GCACCCACAT CTGGCGCTGC ACGGGCATTT GCCGAGAGCG CTCACCCTTT 
TGGGGCTATG TGAAGCGGAC CAGCAACCGA GCGCCCGGTC CGAACGATCA GTGGTGGAAG 
ATGCATCAAC GTGAGTGCAG CGGCACCTTT TTGAAGTTGA GTGAACCCCC GAAGGCCCCC 
AAGCCGGAGA CCAAGTCAAG GGCTAAGAAG TCCACACCGG CGGTGGCCGC GCCCAAGGAA 
GACATACGCA ACTGGTTCGG CCAGCCGTCA GCCAAGAAAG CGGCCACCGG CACAGTCTTA 
CTAGGCGATC TGGCGAAGGC GGTGCCGCCG ACTTCTTATC CCGGACTCTC TTCCGAACCC 
TTTGCATTGC CCAAGACACC AGCTGCTGGC ACAAAAGGCA ACAAAAGCGG CGGATTCAAT 
GAACTGGACA AATCAGGTTC GAAAGCCACC ACTGAAATGA GCGGACAGGG CTACAGTCTT 
GGTGGCTCTA GCGCTATAGA ACACATAGAT CTTAGTGACA CTGATGTTCC CGATTCCGCT 
AAACTTCGTC AAATTCGACT TCAGCGTTTT AGCGAAGATG CTTCCAACAA GCGACAGAAT 
AAGAAAAAGA GGCGTCGTGT TTCCAGCGAC AGCGAGATAA TCAACTGGGA GTCCTACGAT 
GATGACGTAA TGGTCAGGGA TGTTCATACG CCCATCGTCA ACTTGGTCGA AAGCTCTAGT 
GATGAAGAGG AGATGAAGAT GCCAGCTGGC AGAAAAACCA TGACCAGTCA GGAGCGCACG 
CAGATCATCA AGCGCCAGGT AATGGAGGAT GAGTTGACCT TGTGTGAGGA CGATATCCTC 
TTAATTGATG ATGAATACGA CGATGAGGTG GCCGCAAATG AATCTTTAAC AGCCGCCACG 
GAGCTAGCTG ACCAATCCAT TATAGATGAT ATTTTTGGAG AGGACACCCT GCTGAAGGAG 
TTCCAGCGTG AGAACGACGT GCAGCCCAGT TGCTCAAGCT ACGCCAAAAG AATTGAGAAC 
GACATCGTAT CTTGTCCCAT TTGCTTTGAG AAAATGAGGC GCACTGAGTT GTCCAATCAC 
TTCGATGGCT GCGCCATTAT GGTGCGCTTG GAACCGCCAT CGTTTAAGCC TAAAAACCGC 
CGAACAACTG CTTCCTTAAC CTCAAAGGAC TCGTCGGGAG CTTCCACATC ACGCCGCGCT 
AAATCGAAAG CAAAGTCATC AAAAGGAATT CTTCGCAGCT CCGGTTACAC AGAAGGGGAG 
ATCGACAAGC TCAACCTGAG CTCCTCTACC GACTCGTCTA CTCTGCCGTC TAGCGAGGAC 
GAACTTACTC CGCGTCAGCG CCGCCAGCGC AACCTCTTCA AAGAGACCGT AGGCTGCCCC 
AGATGTGGCC TGGAGATCAT GCCTCTACAC CTGAAGGTCC ATGGATCCGT CTGCGCTGGT 
CGCAAGAAGC GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mh cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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