Nadsyn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nadsyn
Entrez Gene ID	32328
Full Name	CG9940 gene product from transcript CG9940-RB
Synonyms	145027_at,Dmel\CG9940,NADS,Q9VYA0
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12B2-12B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298295.1	NP_001285224.1	NAD synthetase, isoform C
NM_132685.2	NP_572913.1	NAD synthetase, isoform A
NM_167378.2	NP_727727.1	NAD synthetase, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NADSYN1	XP_001174076.2
Drosophila melanogaster (fruit fly)	CG9940	NP_727727.1
Saccharomyces cerevisiae (baker's yeast)	QNS1	NP_011941.1
	NADSYN1	XP_001098992.2
Canis lupus familiaris (dog)	NADSYN1	XP_540795.4
Gallus gallus (chicken)	NADSYN1	NP_001006465.1
Eremothecium gossypii	AGOS_AGR358W	NP_987024.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.02	NP_587771.1
Neurospora crassa	NCU04648	XP_959191.1
Arabidopsis thaliana (thale cress)	AT1G55090	NP_175906.1
Oryza sativa (rice)	Os07g0167100	NP_001058975.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000112	XP_311035.4
Bos taurus (cattle)	NADSYN1	NP_001029615.1
Rattus norvegicus (Norway rat)	Nadsyn1	NP_852145.1
Homo sapiens (human)	NADSYN1	NP_060631.2
Mus musculus (house mouse)	Nadsyn1	NP_084497.1
Danio rerio (zebrafish)	nadsyn1	NP_001092723.1
Kluyveromyces lactis	KLLA0D13024g	XP_453643.1
Xenopus tropicalis (tropical clawed frog)	nadsyn1	NP_001120406.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both normal-expression and over-expression of the CG9940 resulted in positive influences on the adaptation of cardiac functions, mobility, and lifespan to exercise in aging Drosophila. Exercise slowed age-related decline of cardiac function, mobility and extent of lifespan in flies, while lower expression of CG9940 led to negative impacts on the adaptation of mobility and lifespan to exercise in Drosophila.
Title: The expression of CG9940 affects the adaptation of cardiac function, mobility, and lifespan to exercise in aging Drosophila.

The following Nadsyn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nadsyn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01532	NM_132685.2 Latest version!	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01532	NM_001298295.1 Latest version!	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01532	NM_167378.2 Latest version!	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nadsyn ( NM_001298295.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285224.1 Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01532
Clone ID Related Accession (Same CDS sequence)	NM_001298295.1 , NM_132685.2 , NM_167378.2
Accession Version	NM_001298295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAD synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285224.1
CDS	701..3064
Translation	MGRKVTVAVSTLNQWALDFEGNMVRILQSILEAKDMGASYRTGPELEVCGYSCEDHFREPDTFLHSWEVLLEVMMSPMCENMLVDVGMPVMHRNVAYNCRVAFFNRQILLIRPKMAMCDDGNYRESRWFTAWTKALQTEEYVLPRMIAQHTGQQTVPFGDAVIATRDTCLGYEICEELWNVRSKHIEMSLAGVELIVNSSGSYMELRKAHITSDLIRNASFKAGGAYLFSNLRGCDGQRVYFNGCSAIALNGEILARSQQFALQDVEVTLATIDLEEIRAYRVSLRSRCTAAASAAEYPRIHCDFEMSTHSDIFKTSTPPLNWPMHTPEEEIALGPACWLWDYLRRSGQGGFFLPLSGGVDSSSSATIVHSMCRQIVQAVQQGDAQVLHDIRQLLADSDYTPDNAAGLCNRLLVTCYMGSVNSSKETRRRAAQLANQLGSYHIEISIDSAVNALLSIFNAVTGLTPRFRTQGGCARQNLALQNMQSRIRMVLAYIFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMDLRRFLTYAKDKFNLPVLESIIDAPPTAELEPLQENGELQQTDEADMGMTYAELSQFGRLRKQSFCGPYSMFCHLVATWKSDLSPKEVAEKVKHFFLCYAINRHKMTVLTPSVHAESYSPDDNRFDHRPFLYRPNWSWQFKAIDDEAEKLQPIYTPSSAQLRPSSEDLLISTQRSSHLDDSKHSSPLSSASASASIDVGISTAAVPLPGAAAPGGLSKKPSGYSKVHVNVLGKIKDRTGIPV

Target ORF information:

RefSeq Version	NM_001298295.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298295.1

ORF Insert Sequence:

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nadsyn ( NM_132685.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572913.1 Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01532
Clone ID Related Accession (Same CDS sequence)	NM_001298295.1 , NM_132685.2 , NM_167378.2
Accession Version	NM_132685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAD synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132685.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572913.1
CDS	702..3065
Translation	MGRKVTVAVSTLNQWALDFEGNMVRILQSILEAKDMGASYRTGPELEVCGYSCEDHFREPDTFLHSWEVLLEVMMSPMCENMLVDVGMPVMHRNVAYNCRVAFFNRQILLIRPKMAMCDDGNYRESRWFTAWTKALQTEEYVLPRMIAQHTGQQTVPFGDAVIATRDTCLGYEICEELWNVRSKHIEMSLAGVELIVNSSGSYMELRKAHITSDLIRNASFKAGGAYLFSNLRGCDGQRVYFNGCSAIALNGEILARSQQFALQDVEVTLATIDLEEIRAYRVSLRSRCTAAASAAEYPRIHCDFEMSTHSDIFKTSTPPLNWPMHTPEEEIALGPACWLWDYLRRSGQGGFFLPLSGGVDSSSSATIVHSMCRQIVQAVQQGDAQVLHDIRQLLADSDYTPDNAAGLCNRLLVTCYMGSVNSSKETRRRAAQLANQLGSYHIEISIDSAVNALLSIFNAVTGLTPRFRTQGGCARQNLALQNMQSRIRMVLAYIFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMDLRRFLTYAKDKFNLPVLESIIDAPPTAELEPLQENGELQQTDEADMGMTYAELSQFGRLRKQSFCGPYSMFCHLVATWKSDLSPKEVAEKVKHFFLCYAINRHKMTVLTPSVHAESYSPDDNRFDHRPFLYRPNWSWQFKAIDDEAEKLQPIYTPSSAQLRPSSEDLLISTQRSSHLDDSKHSSPLSSASASASIDVGISTAAVPLPGAAAPGGLSKKPSGYSKVHVNVLGKIKDRTGIPV

Target ORF information:

RefSeq Version	NM_132685.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132685.2

ORF Insert Sequence:

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nadsyn ( NM_167378.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727727.1 Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant B, mRNA.

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CloneID	OFa01532
Clone ID Related Accession (Same CDS sequence)	NM_001298295.1 , NM_132685.2 , NM_167378.2
Accession Version	NM_167378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	NAD synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167378.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727727.1
CDS	583..2946
Translation	MGRKVTVAVSTLNQWALDFEGNMVRILQSILEAKDMGASYRTGPELEVCGYSCEDHFREPDTFLHSWEVLLEVMMSPMCENMLVDVGMPVMHRNVAYNCRVAFFNRQILLIRPKMAMCDDGNYRESRWFTAWTKALQTEEYVLPRMIAQHTGQQTVPFGDAVIATRDTCLGYEICEELWNVRSKHIEMSLAGVELIVNSSGSYMELRKAHITSDLIRNASFKAGGAYLFSNLRGCDGQRVYFNGCSAIALNGEILARSQQFALQDVEVTLATIDLEEIRAYRVSLRSRCTAAASAAEYPRIHCDFEMSTHSDIFKTSTPPLNWPMHTPEEEIALGPACWLWDYLRRSGQGGFFLPLSGGVDSSSSATIVHSMCRQIVQAVQQGDAQVLHDIRQLLADSDYTPDNAAGLCNRLLVTCYMGSVNSSKETRRRAAQLANQLGSYHIEISIDSAVNALLSIFNAVTGLTPRFRTQGGCARQNLALQNMQSRIRMVLAYIFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMDLRRFLTYAKDKFNLPVLESIIDAPPTAELEPLQENGELQQTDEADMGMTYAELSQFGRLRKQSFCGPYSMFCHLVATWKSDLSPKEVAEKVKHFFLCYAINRHKMTVLTPSVHAESYSPDDNRFDHRPFLYRPNWSWQFKAIDDEAEKLQPIYTPSSAQLRPSSEDLLISTQRSSHLDDSKHSSPLSSASASASIDVGISTAAVPLPGAAAPGGLSKKPSGYSKVHVNVLGKIKDRTGIPV

Target ORF information:

RefSeq Version	NM_167378.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster NAD synthetase (Nadsyn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167378.2

ORF Insert Sequence:

ATGGGACGCA AGGTGACCGT AGCGGTGTCC ACATTGAATC AATGGGCATT GGACTTTGAG 
GGTAACATGG TGAGGATACT GCAGTCCATT CTGGAGGCCA AGGACATGGG CGCCAGCTAT 
CGCACGGGAC CGGAGCTGGA AGTTTGTGGC TACAGCTGCG AGGACCACTT TCGTGAGCCG 
GACACATTCC TGCACTCGTG GGAAGTGCTG CTGGAGGTGA TGATGTCGCC CATGTGTGAA 
AATATGCTGG TGGACGTGGG CATGCCGGTG ATGCATCGCA ATGTGGCCTA CAATTGTCGG 
GTGGCGTTCT TTAATCGCCA GATTCTGCTC ATCCGGCCCA AGATGGCCAT GTGCGACGAT 
GGCAATTATA GGGAGTCGCG ATGGTTTACC GCCTGGACGA AAGCCCTACA AACGGAGGAG 
TATGTGCTGC CGCGAATGAT TGCCCAGCAT ACGGGTCAGC AGACGGTTCC CTTTGGCGAT 
GCAGTGATAG CCACGAGGGA TACCTGTTTG GGCTACGAGA TCTGCGAAGA GCTGTGGAAT 
GTGCGCAGCA AGCACATCGA GATGTCGTTG GCCGGTGTGG AGCTGATAGT GAACAGCTCC 
GGCAGCTATA TGGAATTGCG AAAGGCGCAT ATCACCAGCG ATCTCATTCG GAATGCCAGC 
TTCAAGGCCG GCGGAGCCTA TCTCTTTAGC AATCTTCGAG GTTGCGATGG TCAGCGAGTG 
TACTTCAATG GTTGCTCGGC CATAGCCTTG AATGGTGAGA TCCTGGCGCG CAGTCAGCAG 
TTCGCACTGC AGGATGTGGA GGTCACGCTG GCCACCATCG ATCTGGAGGA GATACGTGCC 
TATCGTGTTA GCCTGAGATC ACGCTGCACG GCTGCTGCAT CGGCAGCGGA GTATCCAAGG 
ATCCATTGTG ACTTTGAGAT GTCCACGCAC AGTGACATAT TCAAGACGTC GACGCCTCCT 
CTGAATTGGC CAATGCACAC GCCTGAGGAG GAGATTGCCC TGGGACCGGC CTGTTGGCTA 
TGGGATTATC TGAGGAGATC CGGTCAAGGG GGATTTTTCC TGCCCCTAAG CGGTGGTGTC 
GATTCGAGTA GCTCGGCCAC AATAGTGCAC TCCATGTGCC GGCAAATCGT GCAGGCTGTC 
CAGCAAGGCG ACGCCCAGGT GCTTCACGAT ATCCGACAGC TTCTGGCCGA CTCGGACTAT 
ACACCGGATA ATGCCGCCGG ATTGTGCAAT CGATTGCTGG TCACCTGCTA TATGGGCAGT 
GTTAATAGCA GCAAGGAAAC CCGTCGACGG GCAGCCCAAT TGGCCAATCA ACTGGGCAGT 
TACCACATCG AGATTAGCAT TGATTCGGCG GTGAATGCAC TATTGAGCAT ATTCAATGCC 
GTCACTGGTT TAACGCCACG TTTCCGTACC CAGGGTGGAT GTGCTCGACA GAATCTGGCA 
TTGCAGAATA TGCAATCGAG GATACGCATG GTCCTGGCCT ACATCTTTGC CCAGTTGACG 
TTGTGGGTAA GAAACAGGCC CGGTGGCTTG CTCGTCCTGG GCTCGGCCAA TGTGGATGAA 
TCGCTGCGCG GTTATCTCAC CAAATATGAC TGCTCCTCGG CGGACATTAA TCCTATTGGT 
GGGATCTCCA AAATGGATCT ACGCAGGTTT CTGACCTATG CCAAGGATAA ATTCAACCTG 
CCCGTGTTGG AGTCCATTAT CGATGCCCCG CCCACCGCCG AGCTGGAACC GCTGCAGGAG 
AACGGGGAGC TGCAGCAGAC GGACGAGGCG GACATGGGAA TGACCTATGC GGAGCTCTCC 
CAGTTCGGTC GCCTGCGCAA ACAGTCATTT TGCGGACCCT ACAGCATGTT TTGCCACCTT 
GTTGCCACCT GGAAGAGCGA CCTTAGTCCC AAGGAGGTGG CCGAAAAGGT GAAGCACTTC 
TTCCTTTGCT ACGCCATTAA TCGGCACAAG ATGACCGTAC TGACGCCGTC AGTGCACGCG 
GAGAGCTACA GTCCCGATGA TAATCGGTTC GATCATCGCC CCTTCTTGTA TCGACCCAAC 
TGGAGCTGGC AGTTCAAGGC CATTGACGAC GAGGCGGAGA AACTGCAACC GATCTACACA 
CCATCTTCGG CTCAATTGCG CCCCAGCAGC GAGGACCTGT TGATCTCCAC ACAGAGATCC 
TCCCACCTGG ACGACTCCAA GCATTCGTCT CCGTTGTCCT CGGCCTCGGC GTCCGCATCG 
ATTGACGTGG GCATTTCAAC GGCAGCGGTT CCGTTGCCCG GTGCAGCTGC CCCAGGCGGT 
CTCTCCAAGA AGCCCAGTGG TTACTCAAAG GTGCACGTCA ATGTGCTTGG CAAGATCAAG 
GATCGCACCG GTATTCCCGT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nadsyn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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