Nep6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nep6
Entrez Gene ID	32234
Full Name	CG3775 gene product from transcript CG3775-RA
Synonyms	BEST:GH06882,Dmel\CG3775
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11C2-11C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132603.4	NP_572831.2	neprilysin 6, isoform A
NM_001258716.2	NP_001245645.1	neprilysin 6, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nep6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nep6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01433	NM_001258716.2 Latest version!	Drosophila melanogaster neprilysin 6, transcript variant B (Nep6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01433	NM_132603.4 Latest version!	Drosophila melanogaster neprilysin 6, transcript variant A (Nep6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nep6 ( NM_132603.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572831.2 Drosophila melanogaster neprilysin 6, transcript variant A (Nep6), mRNA.

Price & Availability↑

CloneID	OFa01433
Clone ID Related Accession (Same CDS sequence)	NM_132603.4 , NM_001258716.2
Accession Version	NM_132603.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 6, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132603.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCCAC CACTATCATC GATCACCCGG CTGGTAGTGA TCCTCGGCGT GATGCTGCTG 
AAGCTGGCGG CAGCCCAGGA TTGTCAGCAG CAATTGGACA ACGACACTGA TGCGGAGGTG 
AAGCCGTGCG AGAATTTTCA TCAGTATGCC TGCGGAAATT GGTATTCGAA CACTTCGCAG 
CGAATTCTCG GTGCCCACGA TATGCGATCC AAGTGGGTGG CAAATCTGAA GCTTCAGCTT 
ATCCGATATC TAGAGGAATC ATCATCCACC GATAGCTTCA GTGCCAATAC CTCCGTCACC 
ACTTCAACCG CCCAGTTGGG CACATTATAC AGATGCTGCT TGTTGAGTGG CCAGTCGATA 
AGAACCTACA CCGATGCCCT GTCACGAAGT GGTGCCAACT TCCCTCTGCT GGCCAACCAT 
ACTGGTGGAT CCTTCGATTG GGTTCTGGCC AATGCCGCCC TGCGTACGTA CGGAGCTCAG 
GGTCTTTGGC GACTCTTGGT CAAGGACAAT TGGCAGTCGG CGGATCAGAG GATCTTCTAC 
ATGCTGCCAC CCAAGTTCGA TCTACTGGGC CGCGATGATG AATTGAATGA GTTTCTGTAT 
CAGCGCTACC TGAAGATTTT GCTCTTGGAA TTGGGCTTGC GAGTGCGTAG AGCTGCTCTG 
CTGGCCGAGA AATTGGTTGC ATTCGAGAAG AGTCTGCAGA AGCTATTGCC CAACGATGCG 
GAACAGACGT TGGTGCTGCG AAAGCCCCAG AGCCTTAAGG ATTTAGAGTC GCAGCTGCCG 
CGGCTGGAGT TGAAAAGATA CTTTGAGACG ATCCTAGAGG GTATCGATTG GGATTCAAAG 
ACACTGTTAC TGGTGGCGGA TTTGGACTAT CTACAGGGTC TGCAGCGATT ACTGGCGGAG 
CACACTTCGG ATCCCATGGT CCTGTCCACT TGGCTGCTCC TTCAGTTACC CGCTTATTTC 
GAATTGCGAT TACATGATGA CGATAAACTG GGCAACCAGC GGAATCATTG CCTGGAACAG 
TTGAGGAAAC TAATGCCCGG ACAACTGGGA CGTCTGCAAA TGCAATTGGT ATTGGGCGAA 
TCGTATCAGG ATGTCTATGA CACTGCCATA CAGCAGATAG CCGTGCTCTT TCGCGATCTA 
AAGCAGCAAT TTGAGTTGGT ACTAAATGAA ACGGAGGTCT TCAACGAGGA TTTGGTCACA 
CGAAACTTGG CTCGTGATAA ACTGAGGGCG ATGCGTTTGC TAACGCCACG TCTACAGGAT 
CCCATCAATG GTGGTCCTGG CACGCCTCAA CTGGGCGGCA ATTGTGATGC GAACCTATTG 
CAGTTGTCAC TTAGCCAAGC CAAATTGGAA TATCGTCAGG TTTTTGGTGA GCCCGTGACT 
TCGGTGCCAG CGGATCCGTT GTCTGTGAAT GCATACTATC GCCTCAAGTT GAATCGGATT 
GAGTTGCCGC TCGGCCTGAT GGCCACGCCC CTTATACAGC AGCAATGCTC CAAGGAGGCG 
GACACTAGGG CCAAACTATC CGCCGGATTG GGCTACATTC TGGCCCACGA AATGGTGCAC 
GCCTTCGATT TGGATGGCCT TAATTACGAT GCCACCGGTC AGTTGGCCAA TGGCCAGTGG 
TCGGCCAGGG CGATTATCCG TTTCGGTCTG AGAGCCGGTT GCTATTTGGG CGTACGGTAT 
AGTAATGCCA CGCTAACCGT CAACGAGAAT ATCGCGGATA CCGAGGGACT ACGACTGGCC 
CTCGACACAT ATCGCCATCG GAGTGGAGCT ACAAATTTGC GTACCTTCTT TGTGGCCTTT 
GCCCAAAATT GGTGTGGCAC AGTGGCCAGT ACGTCGGGCT TGAGCCAACA TGCCGGCCAT 
GCCGAGCGGG TCAATAATGT AGTGGGCAAT ATGCCGGAAT TTGGGGACAC ATTCGAGTGC 
AAGGCAACCA GCCAGATGAA TCCCGTTAAC AAGTGTCACA TTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572831.2
CDS	48..2015
Translation	MKPPLSSITRLVVILGVMLLKLAAAQDCQQQLDNDTDAEVKPCENFHQYACGNWYSNTSQRILGAHDMRSKWVANLKLQLIRYLEESSSTDSFSANTSVTTSTAQLGTLYRCCLLSGQSIRTYTDALSRSGANFPLLANHTGGSFDWVLANAALRTYGAQGLWRLLVKDNWQSADQRIFYMLPPKFDLLGRDDELNEFLYQRYLKILLLELGLRVRRAALLAEKLVAFEKSLQKLLPNDAEQTLVLRKPQSLKDLESQLPRLELKRYFETILEGIDWDSKTLLLVADLDYLQGLQRLLAEHTSDPMVLSTWLLLQLPAYFELRLHDDDKLGNQRNHCLEQLRKLMPGQLGRLQMQLVLGESYQDVYDTAIQQIAVLFRDLKQQFELVLNETEVFNEDLVTRNLARDKLRAMRLLTPRLQDPINGGPGTPQLGGNCDANLLQLSLSQAKLEYRQVFGEPVTSVPADPLSVNAYYRLKLNRIELPLGLMATPLIQQQCSKEADTRAKLSAGLGYILAHEMVHAFDLDGLNYDATGQLANGQWSARAIIRFGLRAGCYLGVRYSNATLTVNENIADTEGLRLALDTYRHRSGATNLRTFFVAFAQNWCGTVASTSGLSQHAGHAERVNNVVGNMPEFGDTFECKATSQMNPVNKCHIW

Target ORF information:

RefSeq Version	NM_132603.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 6, transcript variant A (Nep6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132603.4

ORF Insert Sequence:

ATGAAGCCAC CACTATCATC GATCACCCGG CTGGTAGTGA TCCTCGGCGT GATGCTGCTG 
AAGCTGGCGG CAGCCCAGGA TTGTCAGCAG CAATTGGACA ACGACACTGA TGCGGAGGTG 
AAGCCGTGCG AGAATTTTCA TCAGTATGCC TGCGGAAATT GGTATTCGAA CACTTCGCAG 
CGAATTCTCG GTGCCCACGA TATGCGATCC AAGTGGGTGG CAAATCTGAA GCTTCAGCTT 
ATCCGATATC TAGAGGAATC ATCATCCACC GATAGCTTCA GTGCCAATAC CTCCGTCACC 
ACTTCAACCG CCCAGTTGGG CACATTATAC AGATGCTGCT TGTTGAGTGG CCAGTCGATA 
AGAACCTACA CCGATGCCCT GTCACGAAGT GGTGCCAACT TCCCTCTGCT GGCCAACCAT 
ACTGGTGGAT CCTTCGATTG GGTTCTGGCC AATGCCGCCC TGCGTACGTA CGGAGCTCAG 
GGTCTTTGGC GACTCTTGGT CAAGGACAAT TGGCAGTCGG CGGATCAGAG GATCTTCTAC 
ATGCTGCCAC CCAAGTTCGA TCTACTGGGC CGCGATGATG AATTGAATGA GTTTCTGTAT 
CAGCGCTACC TGAAGATTTT GCTCTTGGAA TTGGGCTTGC GAGTGCGTAG AGCTGCTCTG 
CTGGCCGAGA AATTGGTTGC ATTCGAGAAG AGTCTGCAGA AGCTATTGCC CAACGATGCG 
GAACAGACGT TGGTGCTGCG AAAGCCCCAG AGCCTTAAGG ATTTAGAGTC GCAGCTGCCG 
CGGCTGGAGT TGAAAAGATA CTTTGAGACG ATCCTAGAGG GTATCGATTG GGATTCAAAG 
ACACTGTTAC TGGTGGCGGA TTTGGACTAT CTACAGGGTC TGCAGCGATT ACTGGCGGAG 
CACACTTCGG ATCCCATGGT CCTGTCCACT TGGCTGCTCC TTCAGTTACC CGCTTATTTC 
GAATTGCGAT TACATGATGA CGATAAACTG GGCAACCAGC GGAATCATTG CCTGGAACAG 
TTGAGGAAAC TAATGCCCGG ACAACTGGGA CGTCTGCAAA TGCAATTGGT ATTGGGCGAA 
TCGTATCAGG ATGTCTATGA CACTGCCATA CAGCAGATAG CCGTGCTCTT TCGCGATCTA 
AAGCAGCAAT TTGAGTTGGT ACTAAATGAA ACGGAGGTCT TCAACGAGGA TTTGGTCACA 
CGAAACTTGG CTCGTGATAA ACTGAGGGCG ATGCGTTTGC TAACGCCACG TCTACAGGAT 
CCCATCAATG GTGGTCCTGG CACGCCTCAA CTGGGCGGCA ATTGTGATGC GAACCTATTG 
CAGTTGTCAC TTAGCCAAGC CAAATTGGAA TATCGTCAGG TTTTTGGTGA GCCCGTGACT 
TCGGTGCCAG CGGATCCGTT GTCTGTGAAT GCATACTATC GCCTCAAGTT GAATCGGATT 
GAGTTGCCGC TCGGCCTGAT GGCCACGCCC CTTATACAGC AGCAATGCTC CAAGGAGGCG 
GACACTAGGG CCAAACTATC CGCCGGATTG GGCTACATTC TGGCCCACGA AATGGTGCAC 
GCCTTCGATT TGGATGGCCT TAATTACGAT GCCACCGGTC AGTTGGCCAA TGGCCAGTGG 
TCGGCCAGGG CGATTATCCG TTTCGGTCTG AGAGCCGGTT GCTATTTGGG CGTACGGTAT 
AGTAATGCCA CGCTAACCGT CAACGAGAAT ATCGCGGATA CCGAGGGACT ACGACTGGCC 
CTCGACACAT ATCGCCATCG GAGTGGAGCT ACAAATTTGC GTACCTTCTT TGTGGCCTTT 
GCCCAAAATT GGTGTGGCAC AGTGGCCAGT ACGTCGGGCT TGAGCCAACA TGCCGGCCAT 
GCCGAGCGGG TCAATAATGT AGTGGGCAAT ATGCCGGAAT TTGGGGACAC ATTCGAGTGC 
AAGGCAACCA GCCAGATGAA TCCCGTTAAC AAGTGTCACA TTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nep6 ( NM_001258716.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245645.1 Drosophila melanogaster neprilysin 6, transcript variant B (Nep6), mRNA.

Price & Availability↑

CloneID	OFa01433
Clone ID Related Accession (Same CDS sequence)	NM_132603.4 , NM_001258716.2
Accession Version	NM_001258716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neprilysin 6, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258716.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCCAC CACTATCATC GATCACCCGG CTGGTAGTGA TCCTCGGCGT GATGCTGCTG 
AAGCTGGCGG CAGCCCAGGA TTGTCAGCAG CAATTGGACA ACGACACTGA TGCGGAGGTG 
AAGCCGTGCG AGAATTTTCA TCAGTATGCC TGCGGAAATT GGTATTCGAA CACTTCGCAG 
CGAATTCTCG GTGCCCACGA TATGCGATCC AAGTGGGTGG CAAATCTGAA GCTTCAGCTT 
ATCCGATATC TAGAGGAATC ATCATCCACC GATAGCTTCA GTGCCAATAC CTCCGTCACC 
ACTTCAACCG CCCAGTTGGG CACATTATAC AGATGCTGCT TGTTGAGTGG CCAGTCGATA 
AGAACCTACA CCGATGCCCT GTCACGAAGT GGTGCCAACT TCCCTCTGCT GGCCAACCAT 
ACTGGTGGAT CCTTCGATTG GGTTCTGGCC AATGCCGCCC TGCGTACGTA CGGAGCTCAG 
GGTCTTTGGC GACTCTTGGT CAAGGACAAT TGGCAGTCGG CGGATCAGAG GATCTTCTAC 
ATGCTGCCAC CCAAGTTCGA TCTACTGGGC CGCGATGATG AATTGAATGA GTTTCTGTAT 
CAGCGCTACC TGAAGATTTT GCTCTTGGAA TTGGGCTTGC GAGTGCGTAG AGCTGCTCTG 
CTGGCCGAGA AATTGGTTGC ATTCGAGAAG AGTCTGCAGA AGCTATTGCC CAACGATGCG 
GAACAGACGT TGGTGCTGCG AAAGCCCCAG AGCCTTAAGG ATTTAGAGTC GCAGCTGCCG 
CGGCTGGAGT TGAAAAGATA CTTTGAGACG ATCCTAGAGG GTATCGATTG GGATTCAAAG 
ACACTGTTAC TGGTGGCGGA TTTGGACTAT CTACAGGGTC TGCAGCGATT ACTGGCGGAG 
CACACTTCGG ATCCCATGGT CCTGTCCACT TGGCTGCTCC TTCAGTTACC CGCTTATTTC 
GAATTGCGAT TACATGATGA CGATAAACTG GGCAACCAGC GGAATCATTG CCTGGAACAG 
TTGAGGAAAC TAATGCCCGG ACAACTGGGA CGTCTGCAAA TGCAATTGGT ATTGGGCGAA 
TCGTATCAGG ATGTCTATGA CACTGCCATA CAGCAGATAG CCGTGCTCTT TCGCGATCTA 
AAGCAGCAAT TTGAGTTGGT ACTAAATGAA ACGGAGGTCT TCAACGAGGA TTTGGTCACA 
CGAAACTTGG CTCGTGATAA ACTGAGGGCG ATGCGTTTGC TAACGCCACG TCTACAGGAT 
CCCATCAATG GTGGTCCTGG CACGCCTCAA CTGGGCGGCA ATTGTGATGC GAACCTATTG 
CAGTTGTCAC TTAGCCAAGC CAAATTGGAA TATCGTCAGG TTTTTGGTGA GCCCGTGACT 
TCGGTGCCAG CGGATCCGTT GTCTGTGAAT GCATACTATC GCCTCAAGTT GAATCGGATT 
GAGTTGCCGC TCGGCCTGAT GGCCACGCCC CTTATACAGC AGCAATGCTC CAAGGAGGCG 
GACACTAGGG CCAAACTATC CGCCGGATTG GGCTACATTC TGGCCCACGA AATGGTGCAC 
GCCTTCGATT TGGATGGCCT TAATTACGAT GCCACCGGTC AGTTGGCCAA TGGCCAGTGG 
TCGGCCAGGG CGATTATCCG TTTCGGTCTG AGAGCCGGTT GCTATTTGGG CGTACGGTAT 
AGTAATGCCA CGCTAACCGT CAACGAGAAT ATCGCGGATA CCGAGGGACT ACGACTGGCC 
CTCGACACAT ATCGCCATCG GAGTGGAGCT ACAAATTTGC GTACCTTCTT TGTGGCCTTT 
GCCCAAAATT GGTGTGGCAC AGTGGCCAGT ACGTCGGGCT TGAGCCAACA TGCCGGCCAT 
GCCGAGCGGG TCAATAATGT AGTGGGCAAT ATGCCGGAAT TTGGGGACAC ATTCGAGTGC 
AAGGCAACCA GCCAGATGAA TCCCGTTAAC AAGTGTCACA TTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245645.1
CDS	48..2015
Translation	MKPPLSSITRLVVILGVMLLKLAAAQDCQQQLDNDTDAEVKPCENFHQYACGNWYSNTSQRILGAHDMRSKWVANLKLQLIRYLEESSSTDSFSANTSVTTSTAQLGTLYRCCLLSGQSIRTYTDALSRSGANFPLLANHTGGSFDWVLANAALRTYGAQGLWRLLVKDNWQSADQRIFYMLPPKFDLLGRDDELNEFLYQRYLKILLLELGLRVRRAALLAEKLVAFEKSLQKLLPNDAEQTLVLRKPQSLKDLESQLPRLELKRYFETILEGIDWDSKTLLLVADLDYLQGLQRLLAEHTSDPMVLSTWLLLQLPAYFELRLHDDDKLGNQRNHCLEQLRKLMPGQLGRLQMQLVLGESYQDVYDTAIQQIAVLFRDLKQQFELVLNETEVFNEDLVTRNLARDKLRAMRLLTPRLQDPINGGPGTPQLGGNCDANLLQLSLSQAKLEYRQVFGEPVTSVPADPLSVNAYYRLKLNRIELPLGLMATPLIQQQCSKEADTRAKLSAGLGYILAHEMVHAFDLDGLNYDATGQLANGQWSARAIIRFGLRAGCYLGVRYSNATLTVNENIADTEGLRLALDTYRHRSGATNLRTFFVAFAQNWCGTVASTSGLSQHAGHAERVNNVVGNMPEFGDTFECKATSQMNPVNKCHIW

Target ORF information:

RefSeq Version	NM_001258716.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neprilysin 6, transcript variant B (Nep6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258716.2

ORF Insert Sequence:

ATGAAGCCAC CACTATCATC GATCACCCGG CTGGTAGTGA TCCTCGGCGT GATGCTGCTG 
AAGCTGGCGG CAGCCCAGGA TTGTCAGCAG CAATTGGACA ACGACACTGA TGCGGAGGTG 
AAGCCGTGCG AGAATTTTCA TCAGTATGCC TGCGGAAATT GGTATTCGAA CACTTCGCAG 
CGAATTCTCG GTGCCCACGA TATGCGATCC AAGTGGGTGG CAAATCTGAA GCTTCAGCTT 
ATCCGATATC TAGAGGAATC ATCATCCACC GATAGCTTCA GTGCCAATAC CTCCGTCACC 
ACTTCAACCG CCCAGTTGGG CACATTATAC AGATGCTGCT TGTTGAGTGG CCAGTCGATA 
AGAACCTACA CCGATGCCCT GTCACGAAGT GGTGCCAACT TCCCTCTGCT GGCCAACCAT 
ACTGGTGGAT CCTTCGATTG GGTTCTGGCC AATGCCGCCC TGCGTACGTA CGGAGCTCAG 
GGTCTTTGGC GACTCTTGGT CAAGGACAAT TGGCAGTCGG CGGATCAGAG GATCTTCTAC 
ATGCTGCCAC CCAAGTTCGA TCTACTGGGC CGCGATGATG AATTGAATGA GTTTCTGTAT 
CAGCGCTACC TGAAGATTTT GCTCTTGGAA TTGGGCTTGC GAGTGCGTAG AGCTGCTCTG 
CTGGCCGAGA AATTGGTTGC ATTCGAGAAG AGTCTGCAGA AGCTATTGCC CAACGATGCG 
GAACAGACGT TGGTGCTGCG AAAGCCCCAG AGCCTTAAGG ATTTAGAGTC GCAGCTGCCG 
CGGCTGGAGT TGAAAAGATA CTTTGAGACG ATCCTAGAGG GTATCGATTG GGATTCAAAG 
ACACTGTTAC TGGTGGCGGA TTTGGACTAT CTACAGGGTC TGCAGCGATT ACTGGCGGAG 
CACACTTCGG ATCCCATGGT CCTGTCCACT TGGCTGCTCC TTCAGTTACC CGCTTATTTC 
GAATTGCGAT TACATGATGA CGATAAACTG GGCAACCAGC GGAATCATTG CCTGGAACAG 
TTGAGGAAAC TAATGCCCGG ACAACTGGGA CGTCTGCAAA TGCAATTGGT ATTGGGCGAA 
TCGTATCAGG ATGTCTATGA CACTGCCATA CAGCAGATAG CCGTGCTCTT TCGCGATCTA 
AAGCAGCAAT TTGAGTTGGT ACTAAATGAA ACGGAGGTCT TCAACGAGGA TTTGGTCACA 
CGAAACTTGG CTCGTGATAA ACTGAGGGCG ATGCGTTTGC TAACGCCACG TCTACAGGAT 
CCCATCAATG GTGGTCCTGG CACGCCTCAA CTGGGCGGCA ATTGTGATGC GAACCTATTG 
CAGTTGTCAC TTAGCCAAGC CAAATTGGAA TATCGTCAGG TTTTTGGTGA GCCCGTGACT 
TCGGTGCCAG CGGATCCGTT GTCTGTGAAT GCATACTATC GCCTCAAGTT GAATCGGATT 
GAGTTGCCGC TCGGCCTGAT GGCCACGCCC CTTATACAGC AGCAATGCTC CAAGGAGGCG 
GACACTAGGG CCAAACTATC CGCCGGATTG GGCTACATTC TGGCCCACGA AATGGTGCAC 
GCCTTCGATT TGGATGGCCT TAATTACGAT GCCACCGGTC AGTTGGCCAA TGGCCAGTGG 
TCGGCCAGGG CGATTATCCG TTTCGGTCTG AGAGCCGGTT GCTATTTGGG CGTACGGTAT 
AGTAATGCCA CGCTAACCGT CAACGAGAAT ATCGCGGATA CCGAGGGACT ACGACTGGCC 
CTCGACACAT ATCGCCATCG GAGTGGAGCT ACAAATTTGC GTACCTTCTT TGTGGCCTTT 
GCCCAAAATT GGTGTGGCAC AGTGGCCAGT ACGTCGGGCT TGAGCCAACA TGCCGGCCAT 
GCCGAGCGGG TCAATAATGT AGTGGGCAAT ATGCCGGAAT TTGGGGACAC ATTCGAGTGC 
AAGGCAACCA GCCAGATGAA TCCCGTTAAC AAGTGTCACA TTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nep6 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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