Tis11 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tis11
Entrez Gene ID	32222
Full Name	Tis11 zinc finger protein
Synonyms	CG4070,DTIS11,Dmel\CG4070,TIS11,TIS11cc1,TIScc1,Ts11,anon-EST:Liang-1.28,clone 1.28,dTIS11,dTTP,dTis11,l(1)G0124,tis-11
General protein information	Preferred Names Tis11 zinc finger protein Names CG4070 gene product from transcript CG4070-RC CG4070-PA CG4070-PC CG4070-PD Tis11 homolog Tis11-PA Tis11-PC Tis11-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11B9-11B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272561.1	NP_001259490.1	Tis11 zinc finger protein, isoform C
NM_167333.4	NP_727633.2	Tis11 zinc finger protein, isoform D
NM_078586.5	NP_511141.2	Tis11 zinc finger protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Tis11 protein can affect post-transcriptional gene expression in adult flies by regulating mRNA decay.
Title: The Drosophila Tis11 protein and its effects on mRNA expression in flies.

the Drosophila melanogaster dTIS11 protein is short-lived due to its rapid ubiquitin-independent degradation by the proteasome.
Title: Rapid proteasomal degradation of posttranscriptional regulators of the TIS11/tristetraprolin family is induced by an intrinsically unstructured region independently of ubiquitination.

Nuclear export of TIS11 proteins is mediated by CRM1 through diverging nuclear export signals, while their nuclear import mechanism may rely on a highly conserved signal whose activity is negatively regulated by ZnF2 folding.
Title: A masked PY-NLS in Drosophila TIS11 and its mammalian homolog tristetraprolin.

overexpression of dTIS11 does not promote the localization of ARE-containing mRNAs in P-bodies but rather decreases the accumulation of CecA1 mRNA in these structures by enhancing the degradation process
Title: dTIS11 Protein-dependent polysomal deadenylation is the key step in AU-rich element-mediated mRNA decay in Drosophila cells.

study identified a novel mRNA target of DTIS11, eya, and provided evidence that DTIS11 regulates eye development by interacting with and destabilizing eya mRNA, especially during embryogenesis
Title: Drosophila eyes absent is a novel mRNA target of the tristetraprolin (TTP) protein DTIS11.

The following Tis11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tis11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01423	NM_078586.5 Latest version!	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa01423	NM_001272561.1 Latest version!	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa01423	NM_167333.4 Latest version!	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tis11 ( NM_001272561.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259490.1 Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01423
Clone ID Related Accession (Same CDS sequence)	NM_001272561.1 , NM_167333.4 , NM_078586.5
Accession Version	NM_001272561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tis11 zinc finger protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259490.1
CDS	385..1695
Translation	MSADILQKSREQDDSHYFERGDISKYVTMNDHLGDFDCNEVRKEIRMLLAHGANLDQQHQQQPHRHHGGLTRTISQPAQLIQQQQQQHQQQQQQQPAVASLVTITENLGNMNLHRKLERTQSEPLPPQQPMNTSRYKTELCRPFEEAGECKYGEKCQFAHGSHELRNVHRHPKYKTEYCRTFHSVGFCPYGPRCHFVHNADEARAQQAAQAAKSSTQSQSQSQQSSSQNFSPKSNQSSNQSSNSSSSSSSSGGGGGGGNSINNNNGSQFYLPLSPPLSMSTGSDRESPTGSLSLSPTNSLTSFPFHDALQHGYLASNGAKSNSSASSTSSASGMGLGMSMGIGQGMIIGQGLGMGHHGPATPPESPNVPISPVHTPPPYDVVVSGSGAGNNSVGSKQLLQKSVSTPMQQEDTPRLPVFNRLSSGVEAYQQQSNLGL

Target ORF information:

RefSeq Version	NM_001272561.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272561.1

ORF Insert Sequence:

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tis11 ( NM_167333.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727633.2 Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa01423
Clone ID Related Accession (Same CDS sequence)	NM_001272561.1 , NM_167333.4 , NM_078586.5
Accession Version	NM_167333.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tis11 zinc finger protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167333.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727633.2
CDS	251..1561
Translation	MSADILQKSREQDDSHYFERGDISKYVTMNDHLGDFDCNEVRKEIRMLLAHGANLDQQHQQQPHRHHGGLTRTISQPAQLIQQQQQQHQQQQQQQPAVASLVTITENLGNMNLHRKLERTQSEPLPPQQPMNTSRYKTELCRPFEEAGECKYGEKCQFAHGSHELRNVHRHPKYKTEYCRTFHSVGFCPYGPRCHFVHNADEARAQQAAQAAKSSTQSQSQSQQSSSQNFSPKSNQSSNQSSNSSSSSSSSGGGGGGGNSINNNNGSQFYLPLSPPLSMSTGSDRESPTGSLSLSPTNSLTSFPFHDALQHGYLASNGAKSNSSASSTSSASGMGLGMSMGIGQGMIIGQGLGMGHHGPATPPESPNVPISPVHTPPPYDVVVSGSGAGNNSVGSKQLLQKSVSTPMQQEDTPRLPVFNRLSSGVEAYQQQSNLGL

Target ORF information:

RefSeq Version	NM_167333.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167333.4

ORF Insert Sequence:

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tis11 ( NM_078586.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511141.2 Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01423
Clone ID Related Accession (Same CDS sequence)	NM_001272561.1 , NM_167333.4 , NM_078586.5
Accession Version	NM_078586.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Tis11 zinc finger protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078586.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511141.2
CDS	385..1695
Translation	MSADILQKSREQDDSHYFERGDISKYVTMNDHLGDFDCNEVRKEIRMLLAHGANLDQQHQQQPHRHHGGLTRTISQPAQLIQQQQQQHQQQQQQQPAVASLVTITENLGNMNLHRKLERTQSEPLPPQQPMNTSRYKTELCRPFEEAGECKYGEKCQFAHGSHELRNVHRHPKYKTEYCRTFHSVGFCPYGPRCHFVHNADEARAQQAAQAAKSSTQSQSQSQQSSSQNFSPKSNQSSNQSSNSSSSSSSSGGGGGGGNSINNNNGSQFYLPLSPPLSMSTGSDRESPTGSLSLSPTNSLTSFPFHDALQHGYLASNGAKSNSSASSTSSASGMGLGMSMGIGQGMIIGQGLGMGHHGPATPPESPNVPISPVHTPPPYDVVVSGSGAGNNSVGSKQLLQKSVSTPMQQEDTPRLPVFNRLSSGVEAYQQQSNLGL

Target ORF information:

RefSeq Version	NM_078586.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Tis11 zinc finger protein (Tis11), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078586.5

ORF Insert Sequence:

ATGTCTGCTG ATATTCTGCA GAAATCAAGA GAGCAGGATG ATTCGCACTA CTTCGAGCGT 
GGCGATATAT CCAAATACGT AACGATGAAC GATCACTTGG GTGATTTCGA TTGCAACGAG 
GTGCGCAAGG AAATAAGGAT GCTGCTCGCC CACGGCGCCA ACTTGGATCA GCAGCACCAG 
CAGCAGCCAC ATCGCCACCA TGGCGGTCTC ACACGAACCA TTTCACAGCC GGCCCAGCTC 
ATCCAGCAGC AGCAGCAGCA ACACCAACAG CAGCAGCAGC AGCAGCCAGC TGTTGCCAGT 
CTGGTGACCA TCACCGAGAA CCTGGGCAAC ATGAACCTGC ACCGAAAGCT GGAGCGAACC 
CAATCGGAGC CACTGCCGCC ACAGCAGCCG ATGAACACAT CCAGATACAA GACCGAGCTG 
TGCCGCCCGT TCGAGGAGGC CGGAGAATGC AAGTACGGCG AGAAGTGCCA GTTCGCCCAT 
GGAAGCCATG AGTTGCGAAA CGTGCACCGT CATCCCAAGT ACAAGACGGA ATACTGCCGC 
ACCTTCCACA GCGTGGGCTT CTGTCCCTAC GGACCGCGCT GTCACTTTGT TCACAATGCG 
GACGAGGCCC GCGCCCAACA GGCGGCCCAG GCAGCCAAGT CCTCCACCCA GTCGCAGTCG 
CAGTCGCAGC AGTCGTCGTC GCAGAACTTC TCGCCGAAGA GCAACCAGAG CAGCAATCAA 
AGTAGCAACA GTAGCAGCAG CAGCAGCAGC AGCGGCGGCG GCGGTGGCGG CGGCAACAGC 
ATCAACAACA ACAACGGTAG CCAATTCTAT CTGCCGCTAA GCCCACCGCT GAGCATGAGC 
ACAGGATCGG ACCGGGAATC GCCCACCGGA TCACTGTCCC TCAGCCCCAC CAACTCGTTG 
ACCAGCTTCC CGTTCCACGA TGCCCTGCAG CATGGATATT TGGCATCGAA TGGCGCCAAG 
AGCAACAGTT CCGCCTCGTC CACATCATCG GCCTCTGGAA TGGGTCTGGG CATGAGCATG 
GGCATCGGCC AGGGCATGAT CATCGGTCAG GGCTTGGGAA TGGGACATCA TGGACCGGCT 
ACACCGCCGG AGAGCCCCAA TGTGCCCATA TCGCCAGTGC ATACACCGCC ACCGTACGAT 
GTGGTGGTCA GTGGATCTGG AGCGGGCAAC AATAGCGTTG GCAGCAAGCA GCTCCTGCAG 
AAGAGCGTCA GCACACCGAT GCAGCAGGAG GATACGCCCA GGTTGCCGGT TTTCAACCGT 
CTCAGCTCCG GTGTGGAGGC CTACCAGCAG CAGTCCAATT TGGGACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tis11 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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