regucalcin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	regucalcin
Entrez Gene ID	32165
Full Name	CG1803 gene product from transcript CG1803-RA
Synonyms	CG1803,Dmel\CG1803,Regucalcin,T1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11A1-11A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167315.2	NP_727587.1	regucalcin, isoform B
NM_167316.2	NP_727588.2	regucalcin, isoform D
NM_167314.2	NP_727586.1	regucalcin, isoform A
NM_132547.3	NP_572775.1	regucalcin, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	regucalcin	NP_727588.2
Kluyveromyces lactis	KLLA0F04301g	XP_455274.1
Eremothecium gossypii	AGOS_ABL098W	NP_982849.1
Bos taurus (cattle)	RGN	NP_776382.1
Homo sapiens (human)	RGN	NP_004674.1
	RGN	XP_001090835.1
Mus musculus (house mouse)	Rgn	NP_033086.1
Danio rerio (zebrafish)	rgn	NP_991309.1
Drosophila melanogaster (fruit fly)	smp-30	NP_731942.1
Saccharomyces cerevisiae (baker's yeast)	YBR053C	NP_009609.1
Magnaporthe oryzae (rice blast fungus)	MGG_05015	XP_003712519.1
Neurospora crassa	NCU00326	XP_957797.1
Xenopus tropicalis (tropical clawed frog)	rgn	NP_001006822.1
Pan troglodytes (chimpanzee)	RGN	XP_003317492.1
Canis lupus familiaris (dog)	RGN	XP_538011.3
Rattus norvegicus (Norway rat)	Rgn	NP_113734.1
Gallus gallus (chicken)	RGN	NP_990060.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following regucalcin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the regucalcin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01367	NM_167315.2 Latest version!	Drosophila melanogaster regucalcin, transcript variant B (regucalcin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01367	NM_132547.3 Latest version!	Drosophila melanogaster regucalcin, transcript variant C (regucalcin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01367	NM_167314.2 Latest version!	Drosophila melanogaster regucalcin, transcript variant A (regucalcin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa01368	NM_167316.2 Latest version!	Drosophila melanogaster regucalcin, transcript variant D (regucalcin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

regucalcin ( NM_167315.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727587.1 Drosophila melanogaster regucalcin, transcript variant B (regucalcin), mRNA.

Price & Availability↑

CloneID	OFa01367
Clone ID Related Accession (Same CDS sequence)	NM_167315.2 , NM_167314.2 , NM_132547.3
Accession Version	NM_167315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regucalcin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167315.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727587.1
CDS	143..1054
Translation	MSYKVEPLPDSYAGLGEGPHWDVARQSLYYVDLEAGSLLRYDYAQNKVYKTKIEGETLAGFVLPVEGRPQEFAVGCGRRVVIVNWDGVSPSAKVVRTLFEVQPLMEKNRLNDAKVDPRGRFFGGTMRYIGDEFEFRHGELYRWEAGGQVSVIKGDVGISNGLAWDEKAKKFYYIDTTDYEVKSYDYDFETGVASNPKVIFNLRKNSPKDHLLPDGLTIDTEGNLYVATFNGATIYKVNPNTGKILLEIKFPTKQITSAAFGGPNLDILYVTTAAKFDQPAPAGTTYKVTGLNATGYPGVNLKV

Target ORF information:

RefSeq Version	NM_167315.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regucalcin, transcript variant B (regucalcin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167315.2

ORF Insert Sequence:

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

regucalcin ( NM_167314.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727586.1 Drosophila melanogaster regucalcin, transcript variant A (regucalcin), mRNA.

Price & Availability↑

CloneID	OFa01367
Clone ID Related Accession (Same CDS sequence)	NM_167315.2 , NM_167314.2 , NM_132547.3
Accession Version	NM_167314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regucalcin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167314.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727586.1
CDS	153..1064
Translation	MSYKVEPLPDSYAGLGEGPHWDVARQSLYYVDLEAGSLLRYDYAQNKVYKTKIEGETLAGFVLPVEGRPQEFAVGCGRRVVIVNWDGVSPSAKVVRTLFEVQPLMEKNRLNDAKVDPRGRFFGGTMRYIGDEFEFRHGELYRWEAGGQVSVIKGDVGISNGLAWDEKAKKFYYIDTTDYEVKSYDYDFETGVASNPKVIFNLRKNSPKDHLLPDGLTIDTEGNLYVATFNGATIYKVNPNTGKILLEIKFPTKQITSAAFGGPNLDILYVTTAAKFDQPAPAGTTYKVTGLNATGYPGVNLKV

Target ORF information:

RefSeq Version	NM_167314.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regucalcin, transcript variant A (regucalcin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167314.2

ORF Insert Sequence:

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

regucalcin ( NM_132547.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572775.1 Drosophila melanogaster regucalcin, transcript variant C (regucalcin), mRNA.

Price & Availability↑

CloneID	OFa01367
Clone ID Related Accession (Same CDS sequence)	NM_167315.2 , NM_167314.2 , NM_132547.3
Accession Version	NM_132547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regucalcin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132547.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572775.1
CDS	99..1010
Translation	MSYKVEPLPDSYAGLGEGPHWDVARQSLYYVDLEAGSLLRYDYAQNKVYKTKIEGETLAGFVLPVEGRPQEFAVGCGRRVVIVNWDGVSPSAKVVRTLFEVQPLMEKNRLNDAKVDPRGRFFGGTMRYIGDEFEFRHGELYRWEAGGQVSVIKGDVGISNGLAWDEKAKKFYYIDTTDYEVKSYDYDFETGVASNPKVIFNLRKNSPKDHLLPDGLTIDTEGNLYVATFNGATIYKVNPNTGKILLEIKFPTKQITSAAFGGPNLDILYVTTAAKFDQPAPAGTTYKVTGLNATGYPGVNLKV

Target ORF information:

RefSeq Version	NM_132547.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regucalcin, transcript variant C (regucalcin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132547.3

ORF Insert Sequence:

ATGTCGTACA AGGTGGAACC ATTGCCCGAT TCCTACGCCG GCCTGGGCGA GGGTCCCCAT 
TGGGATGTGG CCAGGCAGAG CCTGTACTAC GTGGATTTGG AGGCAGGCAG CCTGCTCCGC 
TACGACTATG CGCAGAACAA GGTCTACAAG ACAAAGATCG AGGGCGAAAC CTTGGCCGGA 
TTCGTGCTGC CGGTGGAGGG ACGTCCGCAG GAATTCGCCG TCGGCTGCGG TCGACGCGTG 
GTGATCGTCA ACTGGGATGG CGTCTCGCCC AGCGCCAAGG TGGTGCGCAC ACTGTTCGAG 
GTGCAGCCAC TGATGGAGAA GAATCGTTTG AACGACGCCA AGGTTGATCC CCGTGGTCGC 
TTCTTTGGCG GCACCATGCG CTACATTGGC GATGAGTTCG AGTTCCGTCA CGGCGAGCTG 
TACCGCTGGG AGGCCGGTGG CCAGGTGTCG GTGATCAAGG GCGATGTGGG CATCTCCAAT 
GGACTGGCAT GGGACGAGAA GGCCAAGAAG TTCTACTACA TCGATACCAC CGACTACGAG 
GTGAAGTCGT ATGACTATGA TTTCGAGACC GGCGTGGCTA GCAATCCCAA GGTTATATTC 
AATCTGCGCA AGAATAGTCC CAAGGATCAT CTGCTGCCCG ATGGCCTGAC CATCGATACC 
GAGGGCAACC TGTATGTGGC CACCTTCAAT GGCGCCACCA TCTACAAGGT TAATCCCAAC 
ACTGGCAAGA TTCTGCTTGA GATCAAGTTC CCAACCAAAC AGATTACCTC CGCCGCCTTC 
GGTGGCCCCA ACTTGGACAT CCTGTACGTG ACCACTGCCG CCAAGTTCGA TCAGCCCGCT 
CCAGCTGGCA CCACCTACAA GGTGACCGGA CTGAACGCCA CCGGCTATCC CGGCGTCAAC 
CTGAAGGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

regucalcin ( NM_167316.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727588.2 Drosophila melanogaster regucalcin, transcript variant D (regucalcin), mRNA.

Price & Availability↑

CloneID	OFa01368
Clone ID Related Accession (Same CDS sequence)	NM_167316.2
Accession Version	NM_167316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regucalcin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167316.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGTTAC TGATTCCCGT GATTGCAGTG TGCCTTTTCG GCCTAACGAT GTCGTACAAG 
GTGGAACCAT TGCCCGATTC CTACGCCGGC CTGGGCGAGG GTCCCCATTG GGATGTGGCC 
AGGCAGAGCC TGTACTACGT GGATTTGGAG GCAGGCAGCC TGCTCCGCTA CGACTATGCG 
CAGAACAAGG TCTACAAGAC AAAGATCGAG GGCGAAACCT TGGCCGGATT CGTGCTGCCG 
GTGGAGGGAC GTCCGCAGGA ATTCGCCGTC GGCTGCGGTC GACGCGTGGT GATCGTCAAC 
TGGGATGGCG TCTCGCCCAG CGCCAAGGTG GTGCGCACAC TGTTCGAGGT GCAGCCACTG 
ATGGAGAAGA ATCGTTTGAA CGACGCCAAG GTTGATCCCC GTGGTCGCTT CTTTGGCGGC 
ACCATGCGCT ACATTGGCGA TGAGTTCGAG TTCCGTCACG GCGAGCTGTA CCGCTGGGAG 
GCCGGTGGCC AGGTGTCGGT GATCAAGGGC GATGTGGGCA TCTCCAATGG ACTGGCATGG 
GACGAGAAGG CCAAGAAGTT CTACTACATC GATACCACCG ACTACGAGGT GAAGTCGTAT 
GACTATGATT TCGAGACCGG CGTGGCTAGC AATCCCAAGG TTATATTCAA TCTGCGCAAG 
AATAGTCCCA AGGATCATCT GCTGCCCGAT GGCCTGACCA TCGATACCGA GGGCAACCTG 
TATGTGGCCA CCTTCAATGG CGCCACCATC TACAAGGTTA ATCCCAACAC TGGCAAGATT 
CTGCTTGAGA TCAAGTTCCC AACCAAACAG ATTACCTCCG CCGCCTTCGG TGGCCCCAAC 
TTGGACATCC TGTACGTGAC CACTGCCGCC AAGTTCGATC AGCCCGCTCC AGCTGGCACC 
ACCTACAAGG TGACCGGACT GAACGCCACC GGCTATCCCG GCGTCAACCT GAAGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727588.2
CDS	37..996
Translation	MMLLIPVIAVCLFGLTMSYKVEPLPDSYAGLGEGPHWDVARQSLYYVDLEAGSLLRYDYAQNKVYKTKIEGETLAGFVLPVEGRPQEFAVGCGRRVVIVNWDGVSPSAKVVRTLFEVQPLMEKNRLNDAKVDPRGRFFGGTMRYIGDEFEFRHGELYRWEAGGQVSVIKGDVGISNGLAWDEKAKKFYYIDTTDYEVKSYDYDFETGVASNPKVIFNLRKNSPKDHLLPDGLTIDTEGNLYVATFNGATIYKVNPNTGKILLEIKFPTKQITSAAFGGPNLDILYVTTAAKFDQPAPAGTTYKVTGLNATGYPGVNLKV

Target ORF information:

RefSeq Version	NM_167316.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regucalcin, transcript variant D (regucalcin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167316.2

ORF Insert Sequence:

ATGATGTTAC TGATTCCCGT GATTGCAGTG TGCCTTTTCG GCCTAACGAT GTCGTACAAG 
GTGGAACCAT TGCCCGATTC CTACGCCGGC CTGGGCGAGG GTCCCCATTG GGATGTGGCC 
AGGCAGAGCC TGTACTACGT GGATTTGGAG GCAGGCAGCC TGCTCCGCTA CGACTATGCG 
CAGAACAAGG TCTACAAGAC AAAGATCGAG GGCGAAACCT TGGCCGGATT CGTGCTGCCG 
GTGGAGGGAC GTCCGCAGGA ATTCGCCGTC GGCTGCGGTC GACGCGTGGT GATCGTCAAC 
TGGGATGGCG TCTCGCCCAG CGCCAAGGTG GTGCGCACAC TGTTCGAGGT GCAGCCACTG 
ATGGAGAAGA ATCGTTTGAA CGACGCCAAG GTTGATCCCC GTGGTCGCTT CTTTGGCGGC 
ACCATGCGCT ACATTGGCGA TGAGTTCGAG TTCCGTCACG GCGAGCTGTA CCGCTGGGAG 
GCCGGTGGCC AGGTGTCGGT GATCAAGGGC GATGTGGGCA TCTCCAATGG ACTGGCATGG 
GACGAGAAGG CCAAGAAGTT CTACTACATC GATACCACCG ACTACGAGGT GAAGTCGTAT 
GACTATGATT TCGAGACCGG CGTGGCTAGC AATCCCAAGG TTATATTCAA TCTGCGCAAG 
AATAGTCCCA AGGATCATCT GCTGCCCGAT GGCCTGACCA TCGATACCGA GGGCAACCTG 
TATGTGGCCA CCTTCAATGG CGCCACCATC TACAAGGTTA ATCCCAACAC TGGCAAGATT 
CTGCTTGAGA TCAAGTTCCC AACCAAACAG ATTACCTCCG CCGCCTTCGG TGGCCCCAAC 
TTGGACATCC TGTACGTGAC CACTGCCGCC AAGTTCGATC AGCCCGCTCC AGCTGGCACC 
ACCTACAAGG TGACCGGACT GAACGCCACC GGCTATCCCG GCGTCAACCT GAAGGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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regucalcin cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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