Rgn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rgn
Entrez Gene ID	25106
Full Name	regucalcin
Synonyms	GNL,Rc,Reguc
General protein information	Preferred Names regucalcin Names regucalcin SMP-30 gluconolactonase regucalcin (senescence marker protein-30) senescence marker protein 30
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq11
Summary	binds calcium ions; induces increased activity of the sarcoplasmic reticulum calcium-transporting ATPase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031546.1	NP_113734.1	regucalcin

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	regucalcin	NP_727588.2
Kluyveromyces lactis	KLLA0F04301g	XP_455274.1
Eremothecium gossypii	AGOS_ABL098W	NP_982849.1
Bos taurus (cattle)	RGN	NP_776382.1
Homo sapiens (human)	RGN	NP_004674.1
	RGN	XP_001090835.1
Mus musculus (house mouse)	Rgn	NP_033086.1
Danio rerio (zebrafish)	rgn	NP_991309.1
Drosophila melanogaster (fruit fly)	smp-30	NP_731942.1
Saccharomyces cerevisiae (baker's yeast)	YBR053C	NP_009609.1
Magnaporthe oryzae (rice blast fungus)	MGG_05015	XP_003712519.1
Neurospora crassa	NCU00326	XP_957797.1
Xenopus tropicalis (tropical clawed frog)	rgn	NP_001006822.1
Pan troglodytes (chimpanzee)	RGN	XP_003317492.1
Canis lupus familiaris (dog)	RGN	XP_538011.3
Rattus norvegicus (Norway rat)	Rgn	NP_113734.1
Gallus gallus (chicken)	RGN	NP_990060.1

Gene Ontology
Bibliography

Related articles in PubMed

Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats.
Lin PH, Jian CY, Chou JC, Chen CW, Chen CC, Soong C, Hu S, Lieu FK, Wang PS, Wang SW
Scientific reports632085(2016 Aug)

Suppressed glycolytic metabolism in the prostate of transgenic rats overexpressing calcium-binding protein regucalcin underpins reduced cell proliferation.
Vaz CV, Marques R, Cardoso HJ, Maia CJ, Socorro S
Transgenic research25(2)139-48(2016 Apr)

Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells.
Vaz CV, Rodrigues DB, Socorro S, Maia CJ
Biochimica et biophysica acta1853(10 Pt A)2621-8(2015 Oct)

Transgenic overexpression of regucalcin leads to suppression of thapsigargin- and actinomycin D-induced apoptosis in the testis by modulation of apoptotic pathways.
Correia S, Alves MG, Oliveira PF, Alves MR, van Pelt AM, Cavaco JE, Socorro S
Andrology2(2)290-8(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

n. These results showed the effect of RGN in inhibiting the glycolytic metabolism in rat prostate, which was underpinned by a reduced cell proliferation index. The present findings also suggest that the loss of RGN may predispose to a hyper glycolytic profile and fostered proliferation of prostate cells
Title: Suppressed glycolytic metabolism in the prostate of transgenic rats overexpressing calcium-binding protein regucalcin underpins reduced cell proliferation.

results highlighted the importance of RGN as a regulatory protein in Ca2+-dependent signaling pathways and its deregulation of RGN expression by Ca2+o may contribute for onset and progression of PCa.
Title: Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells.

RGN overexpression protects germ cell from apoptosis induced by noxious stimuli, which could be a relevant mechanism for fertility preservation in situations of oncological treatments.
Title: Transgenic overexpression of regucalcin leads to suppression of thapsigargin- and actinomycin D-induced apoptosis in the testis by modulation of apoptotic pathways.

A diminished influx of Ca2+ in epididymal tissues of RGN-overexpressing rats resulted in compromised sperm motility.
Title: Sperm parameters and epididymis function in transgenic rats overexpressing the Ca2+-binding protein regucalcin: a hidden role for Ca2+ in sperm maturation?

Regucalcin may play a pivotal role in the regulation of Ca(2+) signaling which is stimulated through a neurotransmitter in the brain neurons with aging
Title: Role of regucalcin in brain calcium signaling: involvement in aging.

The following Rgn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rgn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12184	NM_031546.1 Latest version!	Rattus norvegicus regucalcin (Rgn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rgn ( NM_031546.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113734.1 Rattus norvegicus regucalcin (Rgn), mRNA.

Price & Availability↑

CloneID	ORa12184
Clone ID Related Accession (Same CDS sequence)	NM_031546.1
Accession Version	NM_031546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	regucalcin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB037934.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB037934.1, BC078794.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906358 [ECO:0000348] ##Evidence-Data-END##

ATGTCTTCCA TCAAGATTGA ATGTGTTTTA AGGGAGAACT ACAGGTGTGG GGAGTCCCCT 
GTGTGGGAGG AGGCATCAAA GTGTCTGCTG TTTGTAGACA TCCCTTCAAA GACTGTCTGC 
CGATGGGATT CGATCAGCAA TCGAGTGCAG CGAGTTGGTG TAGATGCCCC AGTCAGTTCA 
GTGGCACTTC GACAGTCAGG AGGCTATGTT GCCACCATTG GAACCAAGTT CTGTGCTTTG 
AACTGGGAAG ATCAATCAGT ATTTATCCTA GCCATGGTGG ATGAAGATAA GAAAAACAAT 
CGATTCAATG ATGGGAAGGT GGATCCTGCT GGGAGATACT TTGCTGGTAC CATGGCTGAG 
GAAACGGCCC CAGCTGTTCT GGAGCGGCAC CAAGGGTCCT TGTACTCCCT TTTTCCTGAT 
CACAGTGTGA AGAAATACTT TGACCAAGTG GATATCTCCA ATGGTTTGGA TTGGTCCCTG 
GACCATAAAA TCTTCTACTA CATTGACAGC CTGTCCTACA CTGTGGATGC CTTTGACTAT 
GACCTGCCAA CAGGACAGAT TTCCAACCGC AGAACTGTTT ACAAGATGGA AAAAGATGAA 
CAAATCCCAG ATGGAATGTG CATTGATGTT GAGGGGAAGC TTTGGGTGGC CTGTTACAAT 
GGAGGAAGAG TAATTCGCCT AGATCCTGAG ACAGGGAAAA GACTGCAAAC TGTGAAGTTG 
CCTGTTGATA AAACAACTTC ATGCTGCTTT GGAGGGAAGG ATTACTCTGA AATGTACGTG 
ACATGTGCCA GGGATGGGAT GAGCGCCGAA GGTCTTTTGA GGCAGCCTGA TGCTGGTAAC 
ATTTTCAAGA TAACAGGTCT TGGGGTCAAA GGAATTGCTC CATATTCCTA TGCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113734.1
CDS	99..998
Translation	MSSIKIECVLRENYRCGESPVWEEASKCLLFVDIPSKTVCRWDSISNRVQRVGVDAPVSSVALRQSGGYVATIGTKFCALNWEDQSVFILAMVDEDKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERHQGSLYSLFPDHSVKKYFDQVDISNGLDWSLDHKIFYYIDSLSYTVDAFDYDLPTGQISNRRTVYKMEKDEQIPDGMCIDVEGKLWVACYNGGRVIRLDPETGKRLQTVKLPVDKTTSCCFGGKDYSEMYVTCARDGMSAEGLLRQPDAGNIFKITGLGVKGIAPYSYAG

Target ORF information:

RefSeq Version	NM_031546.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regucalcin (Rgn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031546.1

ORF Insert Sequence:

ATGTCTTCCA TCAAGATTGA ATGTGTTTTA AGGGAGAACT ACAGGTGTGG GGAGTCCCCT 
GTGTGGGAGG AGGCATCAAA GTGTCTGCTG TTTGTAGACA TCCCTTCAAA GACTGTCTGC 
CGATGGGATT CGATCAGCAA TCGAGTGCAG CGAGTTGGTG TAGATGCCCC AGTCAGTTCA 
GTGGCACTTC GACAGTCAGG AGGCTATGTT GCCACCATTG GAACCAAGTT CTGTGCTTTG 
AACTGGGAAG ATCAATCAGT ATTTATCCTA GCCATGGTGG ATGAAGATAA GAAAAACAAT 
CGATTCAATG ATGGGAAGGT GGATCCTGCT GGGAGATACT TTGCTGGTAC CATGGCTGAG 
GAAACGGCCC CAGCTGTTCT GGAGCGGCAC CAAGGGTCCT TGTACTCCCT TTTTCCTGAT 
CACAGTGTGA AGAAATACTT TGACCAAGTG GATATCTCCA ATGGTTTGGA TTGGTCCCTG 
GACCATAAAA TCTTCTACTA CATTGACAGC CTGTCCTACA CTGTGGATGC CTTTGACTAT 
GACCTGCCAA CAGGACAGAT TTCCAACCGC AGAACTGTTT ACAAGATGGA AAAAGATGAA 
CAAATCCCAG ATGGAATGTG CATTGATGTT GAGGGGAAGC TTTGGGTGGC CTGTTACAAT 
GGAGGAAGAG TAATTCGCCT AGATCCTGAG ACAGGGAAAA GACTGCAAAC TGTGAAGTTG 
CCTGTTGATA AAACAACTTC ATGCTGCTTT GGAGGGAAGG ATTACTCTGA AATGTACGTG 
ACATGTGCCA GGGATGGGAT GAGCGCCGAA GGTCTTTTGA GGCAGCCTGA TGCTGGTAAC 
ATTTTCAAGA TAACAGGTCT TGGGGTCAAA GGAATTGCTC CATATTCCTA TGCAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Induction of renal senescence marker protein-30 (SMP30) expression by testosterone and its contribution to urinary calcium absorption in male rats.
Scientific reports632085(2016 Aug)
Lin PH,Jian CY,Chou JC,Chen CW,Chen CC,Soong C,Hu S,Lieu FK,Wang PS,Wang SW

Suppressed glycolytic metabolism in the prostate of transgenic rats overexpressing calcium-binding protein regucalcin underpins reduced cell proliferation.
Transgenic research25(2)139-48(2016 Apr)
Vaz CV,Marques R,Cardoso HJ,Maia CJ,Socorro S

Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells.
Biochimica et biophysica acta1853(10 Pt A)2621-8(2015 Oct)
Vaz CV,Rodrigues DB,Socorro S,Maia CJ

Transgenic overexpression of regucalcin leads to suppression of thapsigargin- and actinomycin D-induced apoptosis in the testis by modulation of apoptotic pathways.
Andrology2(2)290-8(2014 Mar)
Correia S,Alves MG,Oliveira PF,Alves MR,van Pelt AM,Cavaco JE,Socorro S

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Rgn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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