dy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dy
Entrez Gene ID	32130
Full Name	dusky
Synonyms	CG9355,Dmel\CG9355,ala,dy1
General protein information	Preferred Names dusky Names CG9355 gene product from transcript CG9355-RA CG9355-PA alae parvae dy-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10E2-10E2\|1-36.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078575.3	NP_511130.2	dusky

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	dy	NP_511130.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000788	XP_311329.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

dyl is essential for the maintenance of hair integrity.
Title: dusky-like is required to maintain the integrity and planar cell polarity of hairs during the development of the Drosophila wing.

Dusky encodes transmembrane proteins containing a ZP (zona pellucida) domain and is homologous to several vertebrate and invertebrate apical matrix components. Dusky is only expressed in tissues secreting a cuticle.
Title: Drosophila miniature and dusky encode ZP proteins required for cytoskeletal reorganisation during wing morphogenesis.

functions as structural component of the Drosophila cuticulin layer
Title: Molecular analysis of the Drosophila miniature-dusky ( m-dy) gene complex: m-dy mRNAs encode transmembrane proteins with similarity to C. elegans cuticulin.

The following dy gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dy cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01319	NM_078575.3 Latest version!	Drosophila melanogaster dusky (dy), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dy ( NM_078575.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511130.2 Drosophila melanogaster dusky (dy), mRNA.

Price & Availability↑

CloneID	OFa01319
Clone ID Related Accession (Same CDS sequence)	NM_078575.3
Accession Version	NM_078575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dusky
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078575.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAACATC AGCAGCAACA GCAACAGCAA CAGCAGCAGC AGCAGCAACA ACACAAGCAA 
GTGCGCTACA GAAATCTACG AAGAAACGTA GAAATCGGTG CTAAGAAGAT TTGGCTGCCG 
CTTATCATTT TCCTTTCACT GCAGGTCGCA CTTGTCTGCG CGGACGATGT GGAGGTTGTG 
AAGGCGATTG CCGGTGGACC CGACGACGAA CTGACCATCG CACTTGGGGA TCACGAGAAC 
GAGCTGGACC AGATGGCCCA GGAATCGGAG CAGGAGCAGC AGGCACAGGC GCAGCAGCAA 
CAGCAACAAC AGCAGCAGCA GCAGCAACAG CAGCAACAGC AGCAGCATCA GCAATTGCCG 
CAAATCCAGA TGCAGTTGCC ACAATCGGTG CCCAAGGTGC AGGTGCACTA CCAGCACAAT 
ATGCCACAGC CGCCTCCAAA TGTGGCACTT ACCATGCGAA ACAATCACCA GAACATTCCG 
CTGAGCTTTG GCCAGCCGTT CGGACCACCA CCGCCACCGG GTGCCCAGTT CTACAAGGGA 
CCACCTCCGC CACAGCTGCA GCAGCGACCG CAGCCACCGC CGCAGTTGCA ATTGCAGGTG 
CAGCCGCAAC AGCAGCAGCA GCAGCAGCAG CAGCATACGC AGCAGCAGGG TCAGCAGCAG 
CAGGTGCAGG TGCCCGACTT GAGTGTGGGC GGATCGAGCA ACAATGAGAT ATGGGCGGCA 
CCTGTACAGG ATATGCCCAA AATTGTCTCC CTGGACGTCA AGTGCGAGAA GAACGGGATG 
AAGGTGTTCG TGCAGTTCGA CAAACCCTTC AATGGCATTG TGTTCTCCAA GGGTCACTAC 
AGCAACATGA ACTGCGTCCA CTTGCCATCG GGTTTGGGAC GCAGCTCGGC GAGCTTTGAT 
ATTGGACTGC ACGAGTGCGG CACCGCCGGC AATACGGACA ACTATGGCCA GGGTTATGGC 
CATGAGGCAG GCAGCACGGG AGCAGGCACC TACTTCGAGA ACATCATCGT CATCCAGTAC 
GATCCGCAGG TGCAGGAGGT GTGGGATCAG GCCAGGAAAC TCCGTTGCAC GTGGCATGAT 
CAGTACGAGA AGAGCGTGAC CTTCCGTCCA TTCCCGGTGG ATATGCTGGA TGTGGTGAGG 
GCCGATTTTG CTGGCGATAA CGTTGGCTGC TGGATGCAAA TCCAGGTGGG CAAGGGTCCG 
TGGGCATCCG AGGTATCTGG ACTGGTTAAG ATCGGCCAGA CGATGACCAT GGTGCTGGCC 
ATCAAGGATG ACGACTCCAA GTTCGATATG CTGGTGAGGA ATTGTGTGGC GCACGACGGC 
AAGAGGGCGC CCATCCAATT GGTCGACCAG CGCGGTTGTG TCACCCGACC CAAGCTAATG 
TCACGCTTCA CCAAGATCAA GAACTTTGGA GCATCTGCGT CCGTGCTGTC CTATGCCCAT 
TTCCAGGCCT TCAAGTTCCC CGATTCGATG GAGGTACATT TCCAGTGCAC CATCCAGATC 
TGTCGCTACC ATTGTCCGGA GCAATGTTCG GCGGAAACGA ATCTGCAGGA TGTCCATCAC 
TTGCAGGTGG GTCCAGAATC GCAGTACGGT CCACCGCCGC AACTTCATGT GGATGCCTAC 
CACGTGGCCA GCGCGATTGG CAAGCGGCGC GACGAGCGAA GGGTGCAGCG ACGTGCCCGA 
GCGGTAGCCG AACCGCAGGT GGGTCTAAAC AGGATCATCA AGGTGGTTTC CTCCGGCGAT 
CTGACCTTCG CCATCGATGA CCAGGCCGGC GGTAATGGAA GCTCAACGAA TGGCGCGAAC 
TCCGGCGTGG ATCGCAGTCC ACAGACCATG GTATTTCCAT TGCGCGAGGA GGGCCTGATC 
TGCATGACCA CGCCGGGCTT TGCCATCACG CTAATCGTGC TCCTGGGTAT CCTTGTCACG 
TCCTGCCTTA CCTCTGCGGT ACTATACGTC CGACTGCGTC CGTTCTCCTC GTTCGCAGGC 
AGCGCCAAGG AGCGGGCTGT GGCCTTCACG AATCCCCAGC TGGCACCGCT GCCCGTCATT 
CAGCTACCGT CGCCGGCGGA TCTGCAGGGA ACGCTCTCCA AGAAGCGGGC GATGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511130.2
CDS	800..2899
Translation	MQHQQQQQQQQQQQQQQHKQVRYRNLRRNVEIGAKKIWLPLIIFLSLQVALVCADDVEVVKAIAGGPDDELTIALGDHENELDQMAQESEQEQQAQAQQQQQQQQQQQQQQQQQQHQQLPQIQMQLPQSVPKVQVHYQHNMPQPPPNVALTMRNNHQNIPLSFGQPFGPPPPPGAQFYKGPPPPQLQQRPQPPPQLQLQVQPQQQQQQQQQHTQQQGQQQQVQVPDLSVGGSSNNEIWAAPVQDMPKIVSLDVKCEKNGMKVFVQFDKPFNGIVFSKGHYSNMNCVHLPSGLGRSSASFDIGLHECGTAGNTDNYGQGYGHEAGSTGAGTYFENIIVIQYDPQVQEVWDQARKLRCTWHDQYEKSVTFRPFPVDMLDVVRADFAGDNVGCWMQIQVGKGPWASEVSGLVKIGQTMTMVLAIKDDDSKFDMLVRNCVAHDGKRAPIQLVDQRGCVTRPKLMSRFTKIKNFGASASVLSYAHFQAFKFPDSMEVHFQCTIQICRYHCPEQCSAETNLQDVHHLQVGPESQYGPPPQLHVDAYHVASAIGKRRDERRVQRRARAVAEPQVGLNRIIKVVSSGDLTFAIDDQAGGNGSSTNGANSGVDRSPQTMVFPLREEGLICMTTPGFAITLIVLLGILVTSCLTSAVLYVRLRPFSSFAGSAKERAVAFTNPQLAPLPVIQLPSPADLQGTLSKKRAMS

Target ORF information:

RefSeq Version	NM_078575.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dusky (dy), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078575.3

ORF Insert Sequence:

ATGCAACATC AGCAGCAACA GCAACAGCAA CAGCAGCAGC AGCAGCAACA ACACAAGCAA 
GTGCGCTACA GAAATCTACG AAGAAACGTA GAAATCGGTG CTAAGAAGAT TTGGCTGCCG 
CTTATCATTT TCCTTTCACT GCAGGTCGCA CTTGTCTGCG CGGACGATGT GGAGGTTGTG 
AAGGCGATTG CCGGTGGACC CGACGACGAA CTGACCATCG CACTTGGGGA TCACGAGAAC 
GAGCTGGACC AGATGGCCCA GGAATCGGAG CAGGAGCAGC AGGCACAGGC GCAGCAGCAA 
CAGCAACAAC AGCAGCAGCA GCAGCAACAG CAGCAACAGC AGCAGCATCA GCAATTGCCG 
CAAATCCAGA TGCAGTTGCC ACAATCGGTG CCCAAGGTGC AGGTGCACTA CCAGCACAAT 
ATGCCACAGC CGCCTCCAAA TGTGGCACTT ACCATGCGAA ACAATCACCA GAACATTCCG 
CTGAGCTTTG GCCAGCCGTT CGGACCACCA CCGCCACCGG GTGCCCAGTT CTACAAGGGA 
CCACCTCCGC CACAGCTGCA GCAGCGACCG CAGCCACCGC CGCAGTTGCA ATTGCAGGTG 
CAGCCGCAAC AGCAGCAGCA GCAGCAGCAG CAGCATACGC AGCAGCAGGG TCAGCAGCAG 
CAGGTGCAGG TGCCCGACTT GAGTGTGGGC GGATCGAGCA ACAATGAGAT ATGGGCGGCA 
CCTGTACAGG ATATGCCCAA AATTGTCTCC CTGGACGTCA AGTGCGAGAA GAACGGGATG 
AAGGTGTTCG TGCAGTTCGA CAAACCCTTC AATGGCATTG TGTTCTCCAA GGGTCACTAC 
AGCAACATGA ACTGCGTCCA CTTGCCATCG GGTTTGGGAC GCAGCTCGGC GAGCTTTGAT 
ATTGGACTGC ACGAGTGCGG CACCGCCGGC AATACGGACA ACTATGGCCA GGGTTATGGC 
CATGAGGCAG GCAGCACGGG AGCAGGCACC TACTTCGAGA ACATCATCGT CATCCAGTAC 
GATCCGCAGG TGCAGGAGGT GTGGGATCAG GCCAGGAAAC TCCGTTGCAC GTGGCATGAT 
CAGTACGAGA AGAGCGTGAC CTTCCGTCCA TTCCCGGTGG ATATGCTGGA TGTGGTGAGG 
GCCGATTTTG CTGGCGATAA CGTTGGCTGC TGGATGCAAA TCCAGGTGGG CAAGGGTCCG 
TGGGCATCCG AGGTATCTGG ACTGGTTAAG ATCGGCCAGA CGATGACCAT GGTGCTGGCC 
ATCAAGGATG ACGACTCCAA GTTCGATATG CTGGTGAGGA ATTGTGTGGC GCACGACGGC 
AAGAGGGCGC CCATCCAATT GGTCGACCAG CGCGGTTGTG TCACCCGACC CAAGCTAATG 
TCACGCTTCA CCAAGATCAA GAACTTTGGA GCATCTGCGT CCGTGCTGTC CTATGCCCAT 
TTCCAGGCCT TCAAGTTCCC CGATTCGATG GAGGTACATT TCCAGTGCAC CATCCAGATC 
TGTCGCTACC ATTGTCCGGA GCAATGTTCG GCGGAAACGA ATCTGCAGGA TGTCCATCAC 
TTGCAGGTGG GTCCAGAATC GCAGTACGGT CCACCGCCGC AACTTCATGT GGATGCCTAC 
CACGTGGCCA GCGCGATTGG CAAGCGGCGC GACGAGCGAA GGGTGCAGCG ACGTGCCCGA 
GCGGTAGCCG AACCGCAGGT GGGTCTAAAC AGGATCATCA AGGTGGTTTC CTCCGGCGAT 
CTGACCTTCG CCATCGATGA CCAGGCCGGC GGTAATGGAA GCTCAACGAA TGGCGCGAAC 
TCCGGCGTGG ATCGCAGTCC ACAGACCATG GTATTTCCAT TGCGCGAGGA GGGCCTGATC 
TGCATGACCA CGCCGGGCTT TGCCATCACG CTAATCGTGC TCCTGGGTAT CCTTGTCACG 
TCCTGCCTTA CCTCTGCGGT ACTATACGTC CGACTGCGTC CGTTCTCCTC GTTCGCAGGC 
AGCGCCAAGG AGCGGGCTGT GGCCTTCACG AATCCCCAGC TGGCACCGCT GCCCGTCATT 
CAGCTACCGT CGCCGGCGGA TCTGCAGGGA ACGCTCTCCA AGAAGCGGGC GATGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dy cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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