stx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stx
Entrez Gene ID	32005
Full Name	stuxnet
Synonyms	CG32676,CG9725,CG9732,Dmel\CG32676
General protein information	Preferred Names stuxnet Names CG32676 gene product from transcript CG32676-RB CG32676-PA CG32676-PB stx-PA stx-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9E1-9E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132420.4	NP_572648.1	stuxnet, isoform A
NM_001272497.2	NP_001259426.1	stuxnet, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Stx facilitates Pc degradation in the proteasome, independent of ubiquitin modification.
Title: Stuxnet Facilitates the Degradation of Polycomb Protein during Development.

The following stx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01250	NM_132420.4 Latest version!	Drosophila melanogaster stuxnet (stx), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01250	NM_001272497.2 Latest version!	Drosophila melanogaster stuxnet (stx), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stx ( NM_132420.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572648.1 Drosophila melanogaster stuxnet (stx), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01250
Clone ID Related Accession (Same CDS sequence)	NM_132420.4 , NM_001272497.2
Accession Version	NM_132420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stuxnet, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132420.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAAAT CAGCGGCCAC GAACTCGGCC ACTGCCGATG GCCAAAACGA GGCAGCAGCA 
GCAGCCGCCG CCGCAGCAAC AGCAGCTGGG TGTGGCAGCA ACAACAGCAG CAGCACCAGC 
AGCAGCAACA ACACTGCAAA CAGCAATAAT GCACTGCAGC GTTTGGCAAC AACAACAAGT 
GCCGTTGTGG CATCCCCGAT GACGATCAAC CTGAACATCA GCACCACCAC CGGCGGCAAT 
TTCGGCGTCA GCGTCGAGCC GCACATCAGT GTCGAGAGTC TGAAGAAGAT CATCGCCAAG 
AAGCTGAAGG TGGCCAAGGA TCGCATCTGT CTGCTGCACC GGGAAAAAGA ACTGCAGGAT 
GGCACGCTCA GAGATCACAA CTTAATGGAC GGTTCCAAGA TCATTTTGAT ACCAAACGTG 
GAAACTGGTT TATTGGCTCA ACGTCCGGAG AACACGGTTA TGCAAGCGCT GGAATCGCTT 
AACGACGCAC AGGTTAATGA TTTTCTCAGC GGCAAGACAC CGCTCAACTT GAGCATGCGC 
CTCGGCGACC ACATGATGCT CATCCAGCTG CAGCTGAGCA CGGTTAATCC GGCGGGCGGC 
GGTGCTGCCA CAGTGGCCCC AGTCGCCGGT GGTGCGAGTG GCTCAAGCAT CAACGCAGCG 
GCAAGCACAA CATCAACGGT GCCTGTAATG CCAGCTGGGA CTGGCTGTTC CGGCGCCAAC 
TCCACATCAT TGTCTTCGTC GGCGGTGCTG GCGGCGGCGG CCGCTGGAGT AGGCGGCAGC 
ACTGGAACCA GTGGCTCCGG CACTTCCTCA TCCTCATCCT CAACGTCATC GTCATCCTCT 
TCGTCTTCGT CTTCGTCGAG AACCCGCTCT TCTGGCCAGC GATCGAGTGG AAGAATCGGA 
CATGGCCACG TGCACAGCCA TCAGCATCCG AGTCTGCATG CTGCGAACTG GCATGGCTTT 
AGTCACGGAC ATGGACATGG TCACAGCCAC GGAAACGGGC ATGGACATCA TCATCATCAC 
CATCACCACC ACCATCATCA CCATCATCAT CACAACGCTT CGGCAGTGGC TGCGGGCAGC 
GGTGGTGGGA TGTCCTCGCT GCGCAAGATG TACGGCGATT TGCCCAGCTC GTCTGGAGCA 
TCTGGCTCTG CTCCAGCAAC TGGCACTGGT CAGTCCCAGA GCTCGTCAAC GCTAAACGGA 
CCCGATCTCA CCAAGCTGCT AATTAAGGGC GGCATTCAGA ATATCGTGAA CAGCTTCGCT 
CTGAAGGATG CCGGCGCCAA TGCTCGTCCT GTGGCCAATG GAGGATCCGG ATCGAGTCCA 
GGGAAGCCTG TGTTAGAGCA GTCGCCCATC AAGTCGCTGT CGAATCTCGT GTCCAGTCCC 
ATCAAGATGA CAACTATCAA AAACATTCCC CTGCCCACGA CGGCTGCCAC AAGTTCCAGT 
TCGAGCTGCA ACTGCAGCTC TGCCGCCCCC AGCTCATCCT CGTCCACTTC CAGTGGAGCC 
TGCGCAACCG GTGGCTGCCA GCAGGATCCG ATAGCGGCCA AGCTGACCTC GTGCTTGTGC 
ACGCGACTAC CCACACCGTC AGTGGCTCCA CCGGTGGCCA CTTTGGTGGC GCCTGCTCCG 
GTGGCCAGAT CCGGATCTAC CAGCTCACCT AGCAAGTGTC CCGAATCGAG TTGCAGTGGA 
GCGAATGCCG GATTTGCAGC TCGTTCGACT CTTGCGGGCA CAAGAGTCAC CTTCTCTGGC 
AATTCGATGC TGCACAAGAC GGGCAATAAT CGCATCACTA GAACCAAGCA TCGTCACTAC 
CACGGGCAGG GCCACGGTCA TGGACATGGA AATGGTCACA GCAGCAGTAG CAGCAGCAGC 
AGCAGCAGCA GTAGTAGCCA CTTTTTTAAT CACGCCTGCG CCGGCAACCA GCCATCGGTT 
AACGCAGCCG CCTTTGCTAC AAGCTCATCA TCGTCTTCCT CGTCCCCGTC ATCTTCCTCC 
TCTTCCCCCT CGAAGCGTCG CAAGCTGGAG CAGGGAATGG GAGCAGGAGC GGTAGGAGCA 
ACGGCCGCAA CCGCAACAGC AGCGGCAACG GTGGCACTAC CAGCCAGCAC CAGCGCCACA 
ACCGCAGAGC CCGATGACTC GCTGCTGGGT GTGTCTGATA CTAGGACTCT CGCTGAGGCA 
TCGAGAAATC TTACCCAGAC ATTGCGTAAA CTCTCCAAGG AGGTGTTCAC CAACAAGATC 
GATTTATCCG GAGCCGGCGT GGTGAGCAGC GGTCGCAGCT CAAGCAGCGG TGCCACCTCG 
AGCGGAGCAG CCAGTGGTGA GGAAGCACCG CGCAAGAGCG GATCGGGAGC CGTGATCGAG 
TCTATGAAGA ACCATGGGAA GGGGATCTAC TCTGGCACCT TTTCGGGCAC ACTCAATCCG 
GCACTACAGG ATCGCTTTGG ACGACCCAAA CGCGACATCT CAACGGTGAT CCATATTCTC 
AACGATCTGT TGAGTGCCAC ACCGCAATAC GGACGTGGTG CACGCATTAG CTTCGAGGCC 
CCGACCACTG GATCGGTTTC GGGATCTGGT TCGGGATCCG GCAGCAGTGG ATCTTCCTCG 
ACTACTTCCT CTGGTAGCGG GCTGCATCAC GGATCGCGTA GCAAAATGTA CTCCTCCAAG 
CACCACAACT GCGCCAAGTG CAACAGCCGC GCCCAGCAGC AACAACAGCA GTCGGCGACT 
CTGGCCTCTG GTAGCGGAGG TAGCTGCTCT TTCCGTGACT GCCCGAGCTA CCACTCCTCC 
TCCGCTGCCA CCAAGGCTTC CACCAGCTCG AAGTCATCGT CGCATAGTTG CTGCCAGACT 
GCTGAGGCAC CCAGCTCTAT GACCAGTCAA TCCAATGGAT GCACATGTCG CTACCGCCGC 
GATGATGGAC AGGGCGAACG GGAGCGTGAG CACACATGCC AGAAGTGCAC CGTGGAGCTG 
GCCAACATGA AGACCCGCTC AAAAATGGAT CAGCTGCGCT TGGTGATGCA GCAGCACAAG 
CAGAAGCGGG AGGCGCGTAA ACTGAAAAGT GGTCCCTATG CCACAGCCGC CTCCGCATCC 
GCGGCGGACA GTCTCTCCTC CATCGCCGCT GGTGGAACAG CTGCTGTTCC GATGGGCGCG 
GCCGCACAGT ACAGCGCCGT GAGCGCCCTG GTGGCTACGC CCCTGCAACC AGCCGCTGTG 
CCCGTTCAGA CGGGGAAAGC TGCACCCGCG AACAGCATCA CTGGCAACAG CAGTAACGCC 
AATGTCAACG GCAATACTTC AACGGCACCT GCAACAGCAG CAACGTCAGC AGCAGCAGCA 
CCAACAGCGG CGCCGCCCAG CGAAGTGTCC CCGAACCACA TTGTGGAGGA GGTGGATACG 
GCGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572648.1
CDS	795..4163
Translation	MDKSAATNSATADGQNEAAAAAAAAATAAGCGSNNSSSTSSSNNTANSNNALQRLATTTSAVVASPMTINLNISTTTGGNFGVSVEPHISVESLKKIIAKKLKVAKDRICLLHREKELQDGTLRDHNLMDGSKIILIPNVETGLLAQRPENTVMQALESLNDAQVNDFLSGKTPLNLSMRLGDHMMLIQLQLSTVNPAGGGAATVAPVAGGASGSSINAAASTTSTVPVMPAGTGCSGANSTSLSSSAVLAAAAAGVGGSTGTSGSGTSSSSSSTSSSSSSSSSSSRTRSSGQRSSGRIGHGHVHSHQHPSLHAANWHGFSHGHGHGHSHGNGHGHHHHHHHHHHHHHHHHNASAVAAGSGGGMSSLRKMYGDLPSSSGASGSAPATGTGQSQSSSTLNGPDLTKLLIKGGIQNIVNSFALKDAGANARPVANGGSGSSPGKPVLEQSPIKSLSNLVSSPIKMTTIKNIPLPTTAATSSSSSCNCSSAAPSSSSSTSSGACATGGCQQDPIAAKLTSCLCTRLPTPSVAPPVATLVAPAPVARSGSTSSPSKCPESSCSGANAGFAARSTLAGTRVTFSGNSMLHKTGNNRITRTKHRHYHGQGHGHGHGNGHSSSSSSSSSSSSSHFFNHACAGNQPSVNAAAFATSSSSSSSSPSSSSSSPSKRRKLEQGMGAGAVGATAATATAAATVALPASTSATTAEPDDSLLGVSDTRTLAEASRNLTQTLRKLSKEVFTNKIDLSGAGVVSSGRSSSSGATSSGAASGEEAPRKSGSGAVIESMKNHGKGIYSGTFSGTLNPALQDRFGRPKRDISTVIHILNDLLSATPQYGRGARISFEAPTTGSVSGSGSGSGSSGSSSTTSSGSGLHHGSRSKMYSSKHHNCAKCNSRAQQQQQQSATLASGSGGSCSFRDCPSYHSSSAATKASTSSKSSSHSCCQTAEAPSSMTSQSNGCTCRYRRDDGQGEREREHTCQKCTVELANMKTRSKMDQLRLVMQQHKQKREARKLKSGPYATAASASAADSLSSIAAGGTAAVPMGAAAQYSAVSALVATPLQPAAVPVQTGKAAPANSITGNSSNANVNGNTSTAPATAATSAAAAPTAAPPSEVSPNHIVEEVDTAA

Target ORF information:

RefSeq Version	NM_132420.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stuxnet (stx), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132420.4

ORF Insert Sequence:

ATGGATAAAT CAGCGGCCAC GAACTCGGCC ACTGCCGATG GCCAAAACGA GGCAGCAGCA 
GCAGCCGCCG CCGCAGCAAC AGCAGCTGGG TGTGGCAGCA ACAACAGCAG CAGCACCAGC 
AGCAGCAACA ACACTGCAAA CAGCAATAAT GCACTGCAGC GTTTGGCAAC AACAACAAGT 
GCCGTTGTGG CATCCCCGAT GACGATCAAC CTGAACATCA GCACCACCAC CGGCGGCAAT 
TTCGGCGTCA GCGTCGAGCC GCACATCAGT GTCGAGAGTC TGAAGAAGAT CATCGCCAAG 
AAGCTGAAGG TGGCCAAGGA TCGCATCTGT CTGCTGCACC GGGAAAAAGA ACTGCAGGAT 
GGCACGCTCA GAGATCACAA CTTAATGGAC GGTTCCAAGA TCATTTTGAT ACCAAACGTG 
GAAACTGGTT TATTGGCTCA ACGTCCGGAG AACACGGTTA TGCAAGCGCT GGAATCGCTT 
AACGACGCAC AGGTTAATGA TTTTCTCAGC GGCAAGACAC CGCTCAACTT GAGCATGCGC 
CTCGGCGACC ACATGATGCT CATCCAGCTG CAGCTGAGCA CGGTTAATCC GGCGGGCGGC 
GGTGCTGCCA CAGTGGCCCC AGTCGCCGGT GGTGCGAGTG GCTCAAGCAT CAACGCAGCG 
GCAAGCACAA CATCAACGGT GCCTGTAATG CCAGCTGGGA CTGGCTGTTC CGGCGCCAAC 
TCCACATCAT TGTCTTCGTC GGCGGTGCTG GCGGCGGCGG CCGCTGGAGT AGGCGGCAGC 
ACTGGAACCA GTGGCTCCGG CACTTCCTCA TCCTCATCCT CAACGTCATC GTCATCCTCT 
TCGTCTTCGT CTTCGTCGAG AACCCGCTCT TCTGGCCAGC GATCGAGTGG AAGAATCGGA 
CATGGCCACG TGCACAGCCA TCAGCATCCG AGTCTGCATG CTGCGAACTG GCATGGCTTT 
AGTCACGGAC ATGGACATGG TCACAGCCAC GGAAACGGGC ATGGACATCA TCATCATCAC 
CATCACCACC ACCATCATCA CCATCATCAT CACAACGCTT CGGCAGTGGC TGCGGGCAGC 
GGTGGTGGGA TGTCCTCGCT GCGCAAGATG TACGGCGATT TGCCCAGCTC GTCTGGAGCA 
TCTGGCTCTG CTCCAGCAAC TGGCACTGGT CAGTCCCAGA GCTCGTCAAC GCTAAACGGA 
CCCGATCTCA CCAAGCTGCT AATTAAGGGC GGCATTCAGA ATATCGTGAA CAGCTTCGCT 
CTGAAGGATG CCGGCGCCAA TGCTCGTCCT GTGGCCAATG GAGGATCCGG ATCGAGTCCA 
GGGAAGCCTG TGTTAGAGCA GTCGCCCATC AAGTCGCTGT CGAATCTCGT GTCCAGTCCC 
ATCAAGATGA CAACTATCAA AAACATTCCC CTGCCCACGA CGGCTGCCAC AAGTTCCAGT 
TCGAGCTGCA ACTGCAGCTC TGCCGCCCCC AGCTCATCCT CGTCCACTTC CAGTGGAGCC 
TGCGCAACCG GTGGCTGCCA GCAGGATCCG ATAGCGGCCA AGCTGACCTC GTGCTTGTGC 
ACGCGACTAC CCACACCGTC AGTGGCTCCA CCGGTGGCCA CTTTGGTGGC GCCTGCTCCG 
GTGGCCAGAT CCGGATCTAC CAGCTCACCT AGCAAGTGTC CCGAATCGAG TTGCAGTGGA 
GCGAATGCCG GATTTGCAGC TCGTTCGACT CTTGCGGGCA CAAGAGTCAC CTTCTCTGGC 
AATTCGATGC TGCACAAGAC GGGCAATAAT CGCATCACTA GAACCAAGCA TCGTCACTAC 
CACGGGCAGG GCCACGGTCA TGGACATGGA AATGGTCACA GCAGCAGTAG CAGCAGCAGC 
AGCAGCAGCA GTAGTAGCCA CTTTTTTAAT CACGCCTGCG CCGGCAACCA GCCATCGGTT 
AACGCAGCCG CCTTTGCTAC AAGCTCATCA TCGTCTTCCT CGTCCCCGTC ATCTTCCTCC 
TCTTCCCCCT CGAAGCGTCG CAAGCTGGAG CAGGGAATGG GAGCAGGAGC GGTAGGAGCA 
ACGGCCGCAA CCGCAACAGC AGCGGCAACG GTGGCACTAC CAGCCAGCAC CAGCGCCACA 
ACCGCAGAGC CCGATGACTC GCTGCTGGGT GTGTCTGATA CTAGGACTCT CGCTGAGGCA 
TCGAGAAATC TTACCCAGAC ATTGCGTAAA CTCTCCAAGG AGGTGTTCAC CAACAAGATC 
GATTTATCCG GAGCCGGCGT GGTGAGCAGC GGTCGCAGCT CAAGCAGCGG TGCCACCTCG 
AGCGGAGCAG CCAGTGGTGA GGAAGCACCG CGCAAGAGCG GATCGGGAGC CGTGATCGAG 
TCTATGAAGA ACCATGGGAA GGGGATCTAC TCTGGCACCT TTTCGGGCAC ACTCAATCCG 
GCACTACAGG ATCGCTTTGG ACGACCCAAA CGCGACATCT CAACGGTGAT CCATATTCTC 
AACGATCTGT TGAGTGCCAC ACCGCAATAC GGACGTGGTG CACGCATTAG CTTCGAGGCC 
CCGACCACTG GATCGGTTTC GGGATCTGGT TCGGGATCCG GCAGCAGTGG ATCTTCCTCG 
ACTACTTCCT CTGGTAGCGG GCTGCATCAC GGATCGCGTA GCAAAATGTA CTCCTCCAAG 
CACCACAACT GCGCCAAGTG CAACAGCCGC GCCCAGCAGC AACAACAGCA GTCGGCGACT 
CTGGCCTCTG GTAGCGGAGG TAGCTGCTCT TTCCGTGACT GCCCGAGCTA CCACTCCTCC 
TCCGCTGCCA CCAAGGCTTC CACCAGCTCG AAGTCATCGT CGCATAGTTG CTGCCAGACT 
GCTGAGGCAC CCAGCTCTAT GACCAGTCAA TCCAATGGAT GCACATGTCG CTACCGCCGC 
GATGATGGAC AGGGCGAACG GGAGCGTGAG CACACATGCC AGAAGTGCAC CGTGGAGCTG 
GCCAACATGA AGACCCGCTC AAAAATGGAT CAGCTGCGCT TGGTGATGCA GCAGCACAAG 
CAGAAGCGGG AGGCGCGTAA ACTGAAAAGT GGTCCCTATG CCACAGCCGC CTCCGCATCC 
GCGGCGGACA GTCTCTCCTC CATCGCCGCT GGTGGAACAG CTGCTGTTCC GATGGGCGCG 
GCCGCACAGT ACAGCGCCGT GAGCGCCCTG GTGGCTACGC CCCTGCAACC AGCCGCTGTG 
CCCGTTCAGA CGGGGAAAGC TGCACCCGCG AACAGCATCA CTGGCAACAG CAGTAACGCC 
AATGTCAACG GCAATACTTC AACGGCACCT GCAACAGCAG CAACGTCAGC AGCAGCAGCA 
CCAACAGCGG CGCCGCCCAG CGAAGTGTCC CCGAACCACA TTGTGGAGGA GGTGGATACG 
GCGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stx ( NM_001272497.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259426.1 Drosophila melanogaster stuxnet (stx), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01250
Clone ID Related Accession (Same CDS sequence)	NM_132420.4 , NM_001272497.2
Accession Version	NM_001272497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stuxnet, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272497.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAAAT CAGCGGCCAC GAACTCGGCC ACTGCCGATG GCCAAAACGA GGCAGCAGCA 
GCAGCCGCCG CCGCAGCAAC AGCAGCTGGG TGTGGCAGCA ACAACAGCAG CAGCACCAGC 
AGCAGCAACA ACACTGCAAA CAGCAATAAT GCACTGCAGC GTTTGGCAAC AACAACAAGT 
GCCGTTGTGG CATCCCCGAT GACGATCAAC CTGAACATCA GCACCACCAC CGGCGGCAAT 
TTCGGCGTCA GCGTCGAGCC GCACATCAGT GTCGAGAGTC TGAAGAAGAT CATCGCCAAG 
AAGCTGAAGG TGGCCAAGGA TCGCATCTGT CTGCTGCACC GGGAAAAAGA ACTGCAGGAT 
GGCACGCTCA GAGATCACAA CTTAATGGAC GGTTCCAAGA TCATTTTGAT ACCAAACGTG 
GAAACTGGTT TATTGGCTCA ACGTCCGGAG AACACGGTTA TGCAAGCGCT GGAATCGCTT 
AACGACGCAC AGGTTAATGA TTTTCTCAGC GGCAAGACAC CGCTCAACTT GAGCATGCGC 
CTCGGCGACC ACATGATGCT CATCCAGCTG CAGCTGAGCA CGGTTAATCC GGCGGGCGGC 
GGTGCTGCCA CAGTGGCCCC AGTCGCCGGT GGTGCGAGTG GCTCAAGCAT CAACGCAGCG 
GCAAGCACAA CATCAACGGT GCCTGTAATG CCAGCTGGGA CTGGCTGTTC CGGCGCCAAC 
TCCACATCAT TGTCTTCGTC GGCGGTGCTG GCGGCGGCGG CCGCTGGAGT AGGCGGCAGC 
ACTGGAACCA GTGGCTCCGG CACTTCCTCA TCCTCATCCT CAACGTCATC GTCATCCTCT 
TCGTCTTCGT CTTCGTCGAG AACCCGCTCT TCTGGCCAGC GATCGAGTGG AAGAATCGGA 
CATGGCCACG TGCACAGCCA TCAGCATCCG AGTCTGCATG CTGCGAACTG GCATGGCTTT 
AGTCACGGAC ATGGACATGG TCACAGCCAC GGAAACGGGC ATGGACATCA TCATCATCAC 
CATCACCACC ACCATCATCA CCATCATCAT CACAACGCTT CGGCAGTGGC TGCGGGCAGC 
GGTGGTGGGA TGTCCTCGCT GCGCAAGATG TACGGCGATT TGCCCAGCTC GTCTGGAGCA 
TCTGGCTCTG CTCCAGCAAC TGGCACTGGT CAGTCCCAGA GCTCGTCAAC GCTAAACGGA 
CCCGATCTCA CCAAGCTGCT AATTAAGGGC GGCATTCAGA ATATCGTGAA CAGCTTCGCT 
CTGAAGGATG CCGGCGCCAA TGCTCGTCCT GTGGCCAATG GAGGATCCGG ATCGAGTCCA 
GGGAAGCCTG TGTTAGAGCA GTCGCCCATC AAGTCGCTGT CGAATCTCGT GTCCAGTCCC 
ATCAAGATGA CAACTATCAA AAACATTCCC CTGCCCACGA CGGCTGCCAC AAGTTCCAGT 
TCGAGCTGCA ACTGCAGCTC TGCCGCCCCC AGCTCATCCT CGTCCACTTC CAGTGGAGCC 
TGCGCAACCG GTGGCTGCCA GCAGGATCCG ATAGCGGCCA AGCTGACCTC GTGCTTGTGC 
ACGCGACTAC CCACACCGTC AGTGGCTCCA CCGGTGGCCA CTTTGGTGGC GCCTGCTCCG 
GTGGCCAGAT CCGGATCTAC CAGCTCACCT AGCAAGTGTC CCGAATCGAG TTGCAGTGGA 
GCGAATGCCG GATTTGCAGC TCGTTCGACT CTTGCGGGCA CAAGAGTCAC CTTCTCTGGC 
AATTCGATGC TGCACAAGAC GGGCAATAAT CGCATCACTA GAACCAAGCA TCGTCACTAC 
CACGGGCAGG GCCACGGTCA TGGACATGGA AATGGTCACA GCAGCAGTAG CAGCAGCAGC 
AGCAGCAGCA GTAGTAGCCA CTTTTTTAAT CACGCCTGCG CCGGCAACCA GCCATCGGTT 
AACGCAGCCG CCTTTGCTAC AAGCTCATCA TCGTCTTCCT CGTCCCCGTC ATCTTCCTCC 
TCTTCCCCCT CGAAGCGTCG CAAGCTGGAG CAGGGAATGG GAGCAGGAGC GGTAGGAGCA 
ACGGCCGCAA CCGCAACAGC AGCGGCAACG GTGGCACTAC CAGCCAGCAC CAGCGCCACA 
ACCGCAGAGC CCGATGACTC GCTGCTGGGT GTGTCTGATA CTAGGACTCT CGCTGAGGCA 
TCGAGAAATC TTACCCAGAC ATTGCGTAAA CTCTCCAAGG AGGTGTTCAC CAACAAGATC 
GATTTATCCG GAGCCGGCGT GGTGAGCAGC GGTCGCAGCT CAAGCAGCGG TGCCACCTCG 
AGCGGAGCAG CCAGTGGTGA GGAAGCACCG CGCAAGAGCG GATCGGGAGC CGTGATCGAG 
TCTATGAAGA ACCATGGGAA GGGGATCTAC TCTGGCACCT TTTCGGGCAC ACTCAATCCG 
GCACTACAGG ATCGCTTTGG ACGACCCAAA CGCGACATCT CAACGGTGAT CCATATTCTC 
AACGATCTGT TGAGTGCCAC ACCGCAATAC GGACGTGGTG CACGCATTAG CTTCGAGGCC 
CCGACCACTG GATCGGTTTC GGGATCTGGT TCGGGATCCG GCAGCAGTGG ATCTTCCTCG 
ACTACTTCCT CTGGTAGCGG GCTGCATCAC GGATCGCGTA GCAAAATGTA CTCCTCCAAG 
CACCACAACT GCGCCAAGTG CAACAGCCGC GCCCAGCAGC AACAACAGCA GTCGGCGACT 
CTGGCCTCTG GTAGCGGAGG TAGCTGCTCT TTCCGTGACT GCCCGAGCTA CCACTCCTCC 
TCCGCTGCCA CCAAGGCTTC CACCAGCTCG AAGTCATCGT CGCATAGTTG CTGCCAGACT 
GCTGAGGCAC CCAGCTCTAT GACCAGTCAA TCCAATGGAT GCACATGTCG CTACCGCCGC 
GATGATGGAC AGGGCGAACG GGAGCGTGAG CACACATGCC AGAAGTGCAC CGTGGAGCTG 
GCCAACATGA AGACCCGCTC AAAAATGGAT CAGCTGCGCT TGGTGATGCA GCAGCACAAG 
CAGAAGCGGG AGGCGCGTAA ACTGAAAAGT GGTCCCTATG CCACAGCCGC CTCCGCATCC 
GCGGCGGACA GTCTCTCCTC CATCGCCGCT GGTGGAACAG CTGCTGTTCC GATGGGCGCG 
GCCGCACAGT ACAGCGCCGT GAGCGCCCTG GTGGCTACGC CCCTGCAACC AGCCGCTGTG 
CCCGTTCAGA CGGGGAAAGC TGCACCCGCG AACAGCATCA CTGGCAACAG CAGTAACGCC 
AATGTCAACG GCAATACTTC AACGGCACCT GCAACAGCAG CAACGTCAGC AGCAGCAGCA 
CCAACAGCGG CGCCGCCCAG CGAAGTGTCC CCGAACCACA TTGTGGAGGA GGTGGATACG 
GCGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259426.1
CDS	795..4163
Translation	MDKSAATNSATADGQNEAAAAAAAAATAAGCGSNNSSSTSSSNNTANSNNALQRLATTTSAVVASPMTINLNISTTTGGNFGVSVEPHISVESLKKIIAKKLKVAKDRICLLHREKELQDGTLRDHNLMDGSKIILIPNVETGLLAQRPENTVMQALESLNDAQVNDFLSGKTPLNLSMRLGDHMMLIQLQLSTVNPAGGGAATVAPVAGGASGSSINAAASTTSTVPVMPAGTGCSGANSTSLSSSAVLAAAAAGVGGSTGTSGSGTSSSSSSTSSSSSSSSSSSRTRSSGQRSSGRIGHGHVHSHQHPSLHAANWHGFSHGHGHGHSHGNGHGHHHHHHHHHHHHHHHHNASAVAAGSGGGMSSLRKMYGDLPSSSGASGSAPATGTGQSQSSSTLNGPDLTKLLIKGGIQNIVNSFALKDAGANARPVANGGSGSSPGKPVLEQSPIKSLSNLVSSPIKMTTIKNIPLPTTAATSSSSSCNCSSAAPSSSSSTSSGACATGGCQQDPIAAKLTSCLCTRLPTPSVAPPVATLVAPAPVARSGSTSSPSKCPESSCSGANAGFAARSTLAGTRVTFSGNSMLHKTGNNRITRTKHRHYHGQGHGHGHGNGHSSSSSSSSSSSSSHFFNHACAGNQPSVNAAAFATSSSSSSSSPSSSSSSPSKRRKLEQGMGAGAVGATAATATAAATVALPASTSATTAEPDDSLLGVSDTRTLAEASRNLTQTLRKLSKEVFTNKIDLSGAGVVSSGRSSSSGATSSGAASGEEAPRKSGSGAVIESMKNHGKGIYSGTFSGTLNPALQDRFGRPKRDISTVIHILNDLLSATPQYGRGARISFEAPTTGSVSGSGSGSGSSGSSSTTSSGSGLHHGSRSKMYSSKHHNCAKCNSRAQQQQQQSATLASGSGGSCSFRDCPSYHSSSAATKASTSSKSSSHSCCQTAEAPSSMTSQSNGCTCRYRRDDGQGEREREHTCQKCTVELANMKTRSKMDQLRLVMQQHKQKREARKLKSGPYATAASASAADSLSSIAAGGTAAVPMGAAAQYSAVSALVATPLQPAAVPVQTGKAAPANSITGNSSNANVNGNTSTAPATAATSAAAAPTAAPPSEVSPNHIVEEVDTAA

Target ORF information:

RefSeq Version	NM_001272497.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stuxnet (stx), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272497.2

ORF Insert Sequence:

ATGGATAAAT CAGCGGCCAC GAACTCGGCC ACTGCCGATG GCCAAAACGA GGCAGCAGCA 
GCAGCCGCCG CCGCAGCAAC AGCAGCTGGG TGTGGCAGCA ACAACAGCAG CAGCACCAGC 
AGCAGCAACA ACACTGCAAA CAGCAATAAT GCACTGCAGC GTTTGGCAAC AACAACAAGT 
GCCGTTGTGG CATCCCCGAT GACGATCAAC CTGAACATCA GCACCACCAC CGGCGGCAAT 
TTCGGCGTCA GCGTCGAGCC GCACATCAGT GTCGAGAGTC TGAAGAAGAT CATCGCCAAG 
AAGCTGAAGG TGGCCAAGGA TCGCATCTGT CTGCTGCACC GGGAAAAAGA ACTGCAGGAT 
GGCACGCTCA GAGATCACAA CTTAATGGAC GGTTCCAAGA TCATTTTGAT ACCAAACGTG 
GAAACTGGTT TATTGGCTCA ACGTCCGGAG AACACGGTTA TGCAAGCGCT GGAATCGCTT 
AACGACGCAC AGGTTAATGA TTTTCTCAGC GGCAAGACAC CGCTCAACTT GAGCATGCGC 
CTCGGCGACC ACATGATGCT CATCCAGCTG CAGCTGAGCA CGGTTAATCC GGCGGGCGGC 
GGTGCTGCCA CAGTGGCCCC AGTCGCCGGT GGTGCGAGTG GCTCAAGCAT CAACGCAGCG 
GCAAGCACAA CATCAACGGT GCCTGTAATG CCAGCTGGGA CTGGCTGTTC CGGCGCCAAC 
TCCACATCAT TGTCTTCGTC GGCGGTGCTG GCGGCGGCGG CCGCTGGAGT AGGCGGCAGC 
ACTGGAACCA GTGGCTCCGG CACTTCCTCA TCCTCATCCT CAACGTCATC GTCATCCTCT 
TCGTCTTCGT CTTCGTCGAG AACCCGCTCT TCTGGCCAGC GATCGAGTGG AAGAATCGGA 
CATGGCCACG TGCACAGCCA TCAGCATCCG AGTCTGCATG CTGCGAACTG GCATGGCTTT 
AGTCACGGAC ATGGACATGG TCACAGCCAC GGAAACGGGC ATGGACATCA TCATCATCAC 
CATCACCACC ACCATCATCA CCATCATCAT CACAACGCTT CGGCAGTGGC TGCGGGCAGC 
GGTGGTGGGA TGTCCTCGCT GCGCAAGATG TACGGCGATT TGCCCAGCTC GTCTGGAGCA 
TCTGGCTCTG CTCCAGCAAC TGGCACTGGT CAGTCCCAGA GCTCGTCAAC GCTAAACGGA 
CCCGATCTCA CCAAGCTGCT AATTAAGGGC GGCATTCAGA ATATCGTGAA CAGCTTCGCT 
CTGAAGGATG CCGGCGCCAA TGCTCGTCCT GTGGCCAATG GAGGATCCGG ATCGAGTCCA 
GGGAAGCCTG TGTTAGAGCA GTCGCCCATC AAGTCGCTGT CGAATCTCGT GTCCAGTCCC 
ATCAAGATGA CAACTATCAA AAACATTCCC CTGCCCACGA CGGCTGCCAC AAGTTCCAGT 
TCGAGCTGCA ACTGCAGCTC TGCCGCCCCC AGCTCATCCT CGTCCACTTC CAGTGGAGCC 
TGCGCAACCG GTGGCTGCCA GCAGGATCCG ATAGCGGCCA AGCTGACCTC GTGCTTGTGC 
ACGCGACTAC CCACACCGTC AGTGGCTCCA CCGGTGGCCA CTTTGGTGGC GCCTGCTCCG 
GTGGCCAGAT CCGGATCTAC CAGCTCACCT AGCAAGTGTC CCGAATCGAG TTGCAGTGGA 
GCGAATGCCG GATTTGCAGC TCGTTCGACT CTTGCGGGCA CAAGAGTCAC CTTCTCTGGC 
AATTCGATGC TGCACAAGAC GGGCAATAAT CGCATCACTA GAACCAAGCA TCGTCACTAC 
CACGGGCAGG GCCACGGTCA TGGACATGGA AATGGTCACA GCAGCAGTAG CAGCAGCAGC 
AGCAGCAGCA GTAGTAGCCA CTTTTTTAAT CACGCCTGCG CCGGCAACCA GCCATCGGTT 
AACGCAGCCG CCTTTGCTAC AAGCTCATCA TCGTCTTCCT CGTCCCCGTC ATCTTCCTCC 
TCTTCCCCCT CGAAGCGTCG CAAGCTGGAG CAGGGAATGG GAGCAGGAGC GGTAGGAGCA 
ACGGCCGCAA CCGCAACAGC AGCGGCAACG GTGGCACTAC CAGCCAGCAC CAGCGCCACA 
ACCGCAGAGC CCGATGACTC GCTGCTGGGT GTGTCTGATA CTAGGACTCT CGCTGAGGCA 
TCGAGAAATC TTACCCAGAC ATTGCGTAAA CTCTCCAAGG AGGTGTTCAC CAACAAGATC 
GATTTATCCG GAGCCGGCGT GGTGAGCAGC GGTCGCAGCT CAAGCAGCGG TGCCACCTCG 
AGCGGAGCAG CCAGTGGTGA GGAAGCACCG CGCAAGAGCG GATCGGGAGC CGTGATCGAG 
TCTATGAAGA ACCATGGGAA GGGGATCTAC TCTGGCACCT TTTCGGGCAC ACTCAATCCG 
GCACTACAGG ATCGCTTTGG ACGACCCAAA CGCGACATCT CAACGGTGAT CCATATTCTC 
AACGATCTGT TGAGTGCCAC ACCGCAATAC GGACGTGGTG CACGCATTAG CTTCGAGGCC 
CCGACCACTG GATCGGTTTC GGGATCTGGT TCGGGATCCG GCAGCAGTGG ATCTTCCTCG 
ACTACTTCCT CTGGTAGCGG GCTGCATCAC GGATCGCGTA GCAAAATGTA CTCCTCCAAG 
CACCACAACT GCGCCAAGTG CAACAGCCGC GCCCAGCAGC AACAACAGCA GTCGGCGACT 
CTGGCCTCTG GTAGCGGAGG TAGCTGCTCT TTCCGTGACT GCCCGAGCTA CCACTCCTCC 
TCCGCTGCCA CCAAGGCTTC CACCAGCTCG AAGTCATCGT CGCATAGTTG CTGCCAGACT 
GCTGAGGCAC CCAGCTCTAT GACCAGTCAA TCCAATGGAT GCACATGTCG CTACCGCCGC 
GATGATGGAC AGGGCGAACG GGAGCGTGAG CACACATGCC AGAAGTGCAC CGTGGAGCTG 
GCCAACATGA AGACCCGCTC AAAAATGGAT CAGCTGCGCT TGGTGATGCA GCAGCACAAG 
CAGAAGCGGG AGGCGCGTAA ACTGAAAAGT GGTCCCTATG CCACAGCCGC CTCCGCATCC 
GCGGCGGACA GTCTCTCCTC CATCGCCGCT GGTGGAACAG CTGCTGTTCC GATGGGCGCG 
GCCGCACAGT ACAGCGCCGT GAGCGCCCTG GTGGCTACGC CCCTGCAACC AGCCGCTGTG 
CCCGTTCAGA CGGGGAAAGC TGCACCCGCG AACAGCATCA CTGGCAACAG CAGTAACGCC 
AATGTCAACG GCAATACTTC AACGGCACCT GCAACAGCAG CAACGTCAGC AGCAGCAGCA 
CCAACAGCGG CGCCGCCCAG CGAAGTGTCC CCGAACCACA TTGTGGAGGA GGTGGATACG 
GCGGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stx cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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