Hmr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmr
Entrez Gene ID	31988
Full Name	Hybrid male rescue
Synonyms	BcDNA:LD22117,CG1619,Dmel\CG1619,HMR,Hmr-mel,Hmr[[mel]],hmr
General protein information	Preferred Names Hybrid male rescue Names CG1619 gene product from transcript CG1619-RB CG1619-PB Hmr-PB hybrid male rescue
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9D3-9D3\|1-31.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132409.3	NP_572637.2	hybrid male rescue

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results find that Hmr does not maintain pure-species function in hybrids.
Title: Limited gene misregulation is exacerbated by allele-specific upregulation in lethal hybrids between Drosophila melanogaster and Drosophila simulans.

Using RNA-Seq analyses we discovered that Hmr and Lhr are required to repress transcripts from satellite DNAs and many families of transposable elements (TEs).
Title: The Hmr and Lhr hybrid incompatibility genes suppress a broad range of heterochromatic repeats.

In(1)AB is mutant for Hmr function, likely due to a missense mutation in an evolutionarily conserved amino acid.
Title: Reduced fertility of Drosophila melanogaster hybrid male rescue (Hmr) mutant females is partially complemented by Hmr orthologs from sibling species.

the frequency of mitotic figures in hybrid male larvae mutant for Hybrid male rescue (Hmr) is increased relative to lethal hybrid males, although not to wild-type levels, and that chromosome morphology in Hmr(-) hybrid males is also not completely normal
Title: Developmental and cell cycle progression defects in Drosophila hybrid males.

findings show Lethal hybrid rescue has functionally diverged in D. simulans & interacts with Hybrid male rescue, which has functionally diverged in D. melanogaster, to cause lethality in F1 hybrid males
Title: Two Dobzhansky-Muller genes interact to cause hybrid lethality in Drosophila.

The following Hmr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03416	NM_132409.3 Latest version!	Drosophila melanogaster hybrid male rescue (Hmr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmr ( NM_132409.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572637.2 Drosophila melanogaster hybrid male rescue (Hmr), mRNA.

Price & Availability↑

CloneID	OFa03416
Clone ID Related Accession (Same CDS sequence)	NM_132409.3
Accession Version	NM_132409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hybrid male rescue
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132409.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAGG AGCCTGTTGC CATTGAAAAT GATTGCCAGA TAATTGTTTC TGACACAATT 
GGTCAGAAGA CGAATGGCGT GGATGACGAT AAGGCCCCAA ATCCGTTCAG CAGATGCACT 
TATCCGGGAT CTCGGGATGG CGGCATTCTG CAAACCTATA GGGCAGAGAG GCTGCTCATC 
GCTTTGGTAC GCCGCCAGCC GCTATTGTAT GACGCCCGGC ATCCGAAATT CCGCGACGTC 
GCTCAAAGGG AGAAACAATG GAAAAAAATC GCCAGCCGCC TGGCAACGAA TGCGACCAAC 
TGCAAGAGAT CCTGGTCGGC GCTGCGATAT AAATATCAGC GCCATGTTCG TCGTCTAAGA 
AACTTTCATC GCAGTGCCAT CCAAAAAGAT CGGGCGGCCC TGCGTTGGCG TCCTTGCATG 
GAATACGAGG AAGAGATGCG CTTCATGTAT ACCCATGTGG CCAGATTCCC ACTGATTGTC 
GACAAGATTC CGACAGAGAT TCTTGAAAAA GAGCATGTGG ATGAGAGCGA AACCCGGCCC 
GATGTGGAGG TCATAGAGAA TCCGCCAATG GACATCATAG ATTTGGATGT CGAGGAGGAC 
CCCACCTATA ACTACCGTTG CAACAGGTTA CAGCGTCGCC TCATAGAGGC AGTCAGTGCC 
TATCCCCTTC TGTACGATAG CACGTGCACG GGCTATCAGA ACACACGACA TCGCTTTCTA 
ATCTGGAGTG CCATTTCAAA CGAAGTCCAT GACAAAGCCA CCAAGCTGAT GAAGTGCTGG 
CTAAAGCTGC AGACTCGCTA CGAATGGGAG TTAATCCATC GCCCACGTCA TATCGGCACT 
TCGGAGCTGT GTCGGTTGAT GGACTTCATG GAACCGCACG TACAGCGCAT GCGCGGCACC 
GTATGCAAGT CTTCCAAGTA CCTGCAAAAC GGTTGGCACG AGCCCATTGA GAATTTCCAC 
ACTGTGATGG CGCTGATCAC CACGATGCGC AACATGCCAG AACTAGTTCA GCTGACGGAG 
GACTCCCTGC ACAACAAGGT GAAACCGCCG CGCTACGACG AATTCTGGCA GAAGGTCGGC 
ATGGAAGTGA TGTGTAGTCA CGAGCACTGC GAGGTCACCT GGCTGGTGCT GCGCGCCTTT 
TACCACGAAT TGCAGGCCGT GCGCTCATCC GGCTATCAGC TGCAGGACAA GTGGTTCTTT 
GAGGGCTCCA TGGGTCTGGC CTTAACGCCA GTTACCTCTC GTTCCACGGC CCGTCCCGCA 
CTAAAGCGGA CAGCCAACGG CGCCATACCA GTGGCGGGGG AGCCTCCGGC CAAAACACCG 
GTGCCGGTAC GTATGCCATT GGCTATTGTC TATCCGTCCA CCTCAAAAGG CAGCAGCACG 
AGCATGACCA ACAGTGCAAG CACTGCCACA AGCATTGGCC CCACCTTCAC CAATGTTAGG 
AGGTCCTCTG GCCCAGTCAT TAACAACACA TCCACCGCCG CCGCCGCAAC GAACAGCGTC 
AGTTCGAGTG TATCGCCCGA TCAAGCTCTT CCCAAGTTTG TGATTCCCAA AATCACGTCC 
GCCATCAGTG TGGGCTCGGG ATATATGATA CCCCTAAAGT TTGTGCCCAA CGTACGAATT 
TTGCCCAAGC CGGCGGCGAC TATGGGAACG GGCGTGTCCC CCATCGCCAC CACCAATCAG 
ATGGCGGGCC CAAACGCAAA GCCGCCTGAA GTGCGTCCAC AGATAACGCA GGGTCCTGGC 
TTACAGGTTA AGCTCCATAC GAAGCCTCAA ATGGCCAGTA GACCGCAAAT GGCACCTGTA 
GTCCCCCGCA CTGTAATTCG AACAACGAAT CCAACCAATC CAGCTCAAAC TCAAGACAGG 
GTATCCGGAG GGCCAATGTC GACACTGGGT GAACTGCTTA GGCCACAAAA TCCATCGCAA 
ATACGTTCAC CAGCTCCGCA ACAAACACTG CCAAATTTTG TACCAATAAC AACATCGAAT 
ATTACAGCTA AAAGCAATCT GCCAACACCC ACTGCGGCTC CAATGGTACG AATACACCAG 
CCGGGACTGA AAAGCGCTTG GGATGGCAAA AGCAATAGCG CTCCGTTCAG TAAGCCAATA 
AGATCACTAT TAACCACATC TTCGCCTGCA GCTGCTCCTA TTGTAGCAAC TGCGGAACCA 
GCGAGAGCAA AAATAATACC ACCAAAGCCC AAATTGCCAG CAACAACATT AGTAAACCCC 
AAAAAATATC AACCAATCGT ACCGGTAGCA GTACTACCTA CAGCAGCAAC CAGGATGGCC 
AAACCTGCAA CAGAACCAGC AACTGAGCTG CCTGTAACTA CGCCACCAAT CGCCACTGGA 
CAAACAACTC GAGTCATACG AAAAACACCA TTATTTCCTA CATCTGCAAC ACAAGCTGCA 
TCTGACTCAT ATGCCACAAT ACAGGGCACA TTAGCAAGCC AGGCTAAAGC TGTGGGAGCA 
GCAATATCAG CACCAGTGGT CAGACCAGCC AAATTGCCGA CAGTAGCCAA GGCAACAACA 
GCATCGCAGT CAGATATAAA AGCAACTGCA GCCGCATCCG CAACCCTAGG CAGTCAGCCG 
GAGAACTTGC CGCTTGGCAA CATAACCGTG TGTCTGCACG AGAGTGATAC CACTGGGAAT 
GAACTGCAAG TTTGGTGCGG AAATCAGGGT ACTCGCTTCA ATCTCAATAT GATTAGGACG 
ACGACGTTGA TCCGCGAGGT GATGGCTGTG CCGCAGCTGC ACAAGGAAGA TCCACAGTTG 
GCGGCCAAGT GTGTTGAGTT CTGGCATTTT ATCGCCAAGA AGTTTCACAT GCCAGAGGAA 
GCTTTGCGCG CATGCTGGAA ATTCTTGGCG GCCAACATGA GTGTGTTTCC AAAAATTGCG 
CCGATGTCGG AACTGATGCG TCCGTTTAAG TACAGCGAAA AAGTGTGGGA AAAATCGAAT 
CGCTTGTTTG GCAAATTTGA CGAGATAGCG CTAAAATACC AGTGGTTGAA TCACAAGAAC 
GTGTTGCCGG AAGTAATACG CTTCTTCGCG AAGTACGAGT ACCTCTACTG CGATTTACGC 
AGGCCGCGAC CTGGCGAAGA TGCTCAAGCT CCGCCCCAGT TGACGAATCA GGAGAAGCAG 
GAGGTGTGGC GGGAGGCCCG CCTTAAATTC CCAAATCTCA ATCATCGGGA TATTTGGTCA 
ATGTTTAAGT TCGCCTTCCG CACATACCTG GATGATTTAG AGCGCGGCAT CGATAATCCC 
TGGCCGCAGA ATTGGTGGCA GGCACTGGAG CAACTCAGGT TTCTGGTCAA TGTGCGCTAC 
CATCCGCTCG AGCCCTACTA CTATATAGTG CACAACAGGA TGTCAGAGGA GGTGAAGCGC 
TGCAAAATGC ACGAGGCTCT AACGTCCTCC GATCATATCG ATAAGACCAA ATTTACACCG 
AGCAAACTTT TAACGGGCTT TTCCAAAGAA CCCAAGCCAT GGGAGACGGA GGAGGCCAAG 
CGCTTGCTTA CCGGCAAGTT GAATAGCGTG AGAAGCACTG AAACTCTCAC TCAAATTGCT 
CCAGCTCCGG CTCTAGCTTT GGCACCCGAT GCCCAACCTA TTCCTGTACC TGATGGCGTT 
TCCGTGTCCA AAAAGACAAG CGAAAGCAAG CCAGCGTCAA ACGCCAACGT CAAGTCTCAT 
AAAGACAAGA ATATCTCCAC CGCCCAAAAG CGGCCGACCC CAGCGAGAAG TGTCACCTGT 
AACAATCTAT CCCCCATTGA GGCGTTTGAG TTGTGTAAGA AATTGCGTAG CCAACCAAAC 
AGCTTCGAGC GGGCGAGCAC TCTGGACAAG CGAGCCGCCT GGGTTCGCGT ATCAAAGGAG 
TTGCATGCCA CAGTGACCGA ATGCCGCCTT GGTCTGCAGT ACGCTCTGCG CGAGATGCGC 
AATCTGAAGA TCGCCGATCC AAGCAATCAA TGTACAATGG GCCACAAGTA CTTGCACCAC 
ATGTCGGAAA TCTATAAGCA GGTGAAGCCG AACGCCCAGC TGACAGTCCG CACGCCACAG 
CAGCTCAACC AGTTAAACGA ATCGCTTGCG AAGAACACTC AGGAAGTTAG CCCGCAGGAG 
TTCCTGCCTG AGATCAATTT GTCCACTTGC AGTCCGGATC TCGTGGTGAA GAATTGGGCC 
TACGCCGTCG GTAACTTGTC CACGGCCAGT CAGGATACAC TGCTCGGCAA GATGACGCAG 
CTGTTCTCTA AATACGCCAA GGTCAATCCG CCACCGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572637.2
CDS	264..4505
Translation	MEEEPVAIENDCQIIVSDTIGQKTNGVDDDKAPNPFSRCTYPGSRDGGILQTYRAERLLIALVRRQPLLYDARHPKFRDVAQREKQWKKIASRLATNATNCKRSWSALRYKYQRHVRRLRNFHRSAIQKDRAALRWRPCMEYEEEMRFMYTHVARFPLIVDKIPTEILEKEHVDESETRPDVEVIENPPMDIIDLDVEEDPTYNYRCNRLQRRLIEAVSAYPLLYDSTCTGYQNTRHRFLIWSAISNEVHDKATKLMKCWLKLQTRYEWELIHRPRHIGTSELCRLMDFMEPHVQRMRGTVCKSSKYLQNGWHEPIENFHTVMALITTMRNMPELVQLTEDSLHNKVKPPRYDEFWQKVGMEVMCSHEHCEVTWLVLRAFYHELQAVRSSGYQLQDKWFFEGSMGLALTPVTSRSTARPALKRTANGAIPVAGEPPAKTPVPVRMPLAIVYPSTSKGSSTSMTNSASTATSIGPTFTNVRRSSGPVINNTSTAAAATNSVSSSVSPDQALPKFVIPKITSAISVGSGYMIPLKFVPNVRILPKPAATMGTGVSPIATTNQMAGPNAKPPEVRPQITQGPGLQVKLHTKPQMASRPQMAPVVPRTVIRTTNPTNPAQTQDRVSGGPMSTLGELLRPQNPSQIRSPAPQQTLPNFVPITTSNITAKSNLPTPTAAPMVRIHQPGLKSAWDGKSNSAPFSKPIRSLLTTSSPAAAPIVATAEPARAKIIPPKPKLPATTLVNPKKYQPIVPVAVLPTAATRMAKPATEPATELPVTTPPIATGQTTRVIRKTPLFPTSATQAASDSYATIQGTLASQAKAVGAAISAPVVRPAKLPTVAKATTASQSDIKATAAASATLGSQPENLPLGNITVCLHESDTTGNELQVWCGNQGTRFNLNMIRTTTLIREVMAVPQLHKEDPQLAAKCVEFWHFIAKKFHMPEEALRACWKFLAANMSVFPKIAPMSELMRPFKYSEKVWEKSNRLFGKFDEIALKYQWLNHKNVLPEVIRFFAKYEYLYCDLRRPRPGEDAQAPPQLTNQEKQEVWREARLKFPNLNHRDIWSMFKFAFRTYLDDLERGIDNPWPQNWWQALEQLRFLVNVRYHPLEPYYYIVHNRMSEEVKRCKMHEALTSSDHIDKTKFTPSKLLTGFSKEPKPWETEEAKRLLTGKLNSVRSTETLTQIAPAPALALAPDAQPIPVPDGVSVSKKTSESKPASNANVKSHKDKNISTAQKRPTPARSVTCNNLSPIEAFELCKKLRSQPNSFERASTLDKRAAWVRVSKELHATVTECRLGLQYALREMRNLKIADPSNQCTMGHKYLHHMSEIYKQVKPNAQLTVRTPQQLNQLNESLAKNTQEVSPQEFLPEINLSTCSPDLVVKNWAYAVGNLSTASQDTLLGKMTQLFSKYAKVNPPPP

Target ORF information:

RefSeq Version	NM_132409.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hybrid male rescue (Hmr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132409.3

ORF Insert Sequence:

ATGGAGGAGG AGCCTGTTGC CATTGAAAAT GATTGCCAGA TAATTGTTTC TGACACAATT 
GGTCAGAAGA CGAATGGCGT GGATGACGAT AAGGCCCCAA ATCCGTTCAG CAGATGCACT 
TATCCGGGAT CTCGGGATGG CGGCATTCTG CAAACCTATA GGGCAGAGAG GCTGCTCATC 
GCTTTGGTAC GCCGCCAGCC GCTATTGTAT GACGCCCGGC ATCCGAAATT CCGCGACGTC 
GCTCAAAGGG AGAAACAATG GAAAAAAATC GCCAGCCGCC TGGCAACGAA TGCGACCAAC 
TGCAAGAGAT CCTGGTCGGC GCTGCGATAT AAATATCAGC GCCATGTTCG TCGTCTAAGA 
AACTTTCATC GCAGTGCCAT CCAAAAAGAT CGGGCGGCCC TGCGTTGGCG TCCTTGCATG 
GAATACGAGG AAGAGATGCG CTTCATGTAT ACCCATGTGG CCAGATTCCC ACTGATTGTC 
GACAAGATTC CGACAGAGAT TCTTGAAAAA GAGCATGTGG ATGAGAGCGA AACCCGGCCC 
GATGTGGAGG TCATAGAGAA TCCGCCAATG GACATCATAG ATTTGGATGT CGAGGAGGAC 
CCCACCTATA ACTACCGTTG CAACAGGTTA CAGCGTCGCC TCATAGAGGC AGTCAGTGCC 
TATCCCCTTC TGTACGATAG CACGTGCACG GGCTATCAGA ACACACGACA TCGCTTTCTA 
ATCTGGAGTG CCATTTCAAA CGAAGTCCAT GACAAAGCCA CCAAGCTGAT GAAGTGCTGG 
CTAAAGCTGC AGACTCGCTA CGAATGGGAG TTAATCCATC GCCCACGTCA TATCGGCACT 
TCGGAGCTGT GTCGGTTGAT GGACTTCATG GAACCGCACG TACAGCGCAT GCGCGGCACC 
GTATGCAAGT CTTCCAAGTA CCTGCAAAAC GGTTGGCACG AGCCCATTGA GAATTTCCAC 
ACTGTGATGG CGCTGATCAC CACGATGCGC AACATGCCAG AACTAGTTCA GCTGACGGAG 
GACTCCCTGC ACAACAAGGT GAAACCGCCG CGCTACGACG AATTCTGGCA GAAGGTCGGC 
ATGGAAGTGA TGTGTAGTCA CGAGCACTGC GAGGTCACCT GGCTGGTGCT GCGCGCCTTT 
TACCACGAAT TGCAGGCCGT GCGCTCATCC GGCTATCAGC TGCAGGACAA GTGGTTCTTT 
GAGGGCTCCA TGGGTCTGGC CTTAACGCCA GTTACCTCTC GTTCCACGGC CCGTCCCGCA 
CTAAAGCGGA CAGCCAACGG CGCCATACCA GTGGCGGGGG AGCCTCCGGC CAAAACACCG 
GTGCCGGTAC GTATGCCATT GGCTATTGTC TATCCGTCCA CCTCAAAAGG CAGCAGCACG 
AGCATGACCA ACAGTGCAAG CACTGCCACA AGCATTGGCC CCACCTTCAC CAATGTTAGG 
AGGTCCTCTG GCCCAGTCAT TAACAACACA TCCACCGCCG CCGCCGCAAC GAACAGCGTC 
AGTTCGAGTG TATCGCCCGA TCAAGCTCTT CCCAAGTTTG TGATTCCCAA AATCACGTCC 
GCCATCAGTG TGGGCTCGGG ATATATGATA CCCCTAAAGT TTGTGCCCAA CGTACGAATT 
TTGCCCAAGC CGGCGGCGAC TATGGGAACG GGCGTGTCCC CCATCGCCAC CACCAATCAG 
ATGGCGGGCC CAAACGCAAA GCCGCCTGAA GTGCGTCCAC AGATAACGCA GGGTCCTGGC 
TTACAGGTTA AGCTCCATAC GAAGCCTCAA ATGGCCAGTA GACCGCAAAT GGCACCTGTA 
GTCCCCCGCA CTGTAATTCG AACAACGAAT CCAACCAATC CAGCTCAAAC TCAAGACAGG 
GTATCCGGAG GGCCAATGTC GACACTGGGT GAACTGCTTA GGCCACAAAA TCCATCGCAA 
ATACGTTCAC CAGCTCCGCA ACAAACACTG CCAAATTTTG TACCAATAAC AACATCGAAT 
ATTACAGCTA AAAGCAATCT GCCAACACCC ACTGCGGCTC CAATGGTACG AATACACCAG 
CCGGGACTGA AAAGCGCTTG GGATGGCAAA AGCAATAGCG CTCCGTTCAG TAAGCCAATA 
AGATCACTAT TAACCACATC TTCGCCTGCA GCTGCTCCTA TTGTAGCAAC TGCGGAACCA 
GCGAGAGCAA AAATAATACC ACCAAAGCCC AAATTGCCAG CAACAACATT AGTAAACCCC 
AAAAAATATC AACCAATCGT ACCGGTAGCA GTACTACCTA CAGCAGCAAC CAGGATGGCC 
AAACCTGCAA CAGAACCAGC AACTGAGCTG CCTGTAACTA CGCCACCAAT CGCCACTGGA 
CAAACAACTC GAGTCATACG AAAAACACCA TTATTTCCTA CATCTGCAAC ACAAGCTGCA 
TCTGACTCAT ATGCCACAAT ACAGGGCACA TTAGCAAGCC AGGCTAAAGC TGTGGGAGCA 
GCAATATCAG CACCAGTGGT CAGACCAGCC AAATTGCCGA CAGTAGCCAA GGCAACAACA 
GCATCGCAGT CAGATATAAA AGCAACTGCA GCCGCATCCG CAACCCTAGG CAGTCAGCCG 
GAGAACTTGC CGCTTGGCAA CATAACCGTG TGTCTGCACG AGAGTGATAC CACTGGGAAT 
GAACTGCAAG TTTGGTGCGG AAATCAGGGT ACTCGCTTCA ATCTCAATAT GATTAGGACG 
ACGACGTTGA TCCGCGAGGT GATGGCTGTG CCGCAGCTGC ACAAGGAAGA TCCACAGTTG 
GCGGCCAAGT GTGTTGAGTT CTGGCATTTT ATCGCCAAGA AGTTTCACAT GCCAGAGGAA 
GCTTTGCGCG CATGCTGGAA ATTCTTGGCG GCCAACATGA GTGTGTTTCC AAAAATTGCG 
CCGATGTCGG AACTGATGCG TCCGTTTAAG TACAGCGAAA AAGTGTGGGA AAAATCGAAT 
CGCTTGTTTG GCAAATTTGA CGAGATAGCG CTAAAATACC AGTGGTTGAA TCACAAGAAC 
GTGTTGCCGG AAGTAATACG CTTCTTCGCG AAGTACGAGT ACCTCTACTG CGATTTACGC 
AGGCCGCGAC CTGGCGAAGA TGCTCAAGCT CCGCCCCAGT TGACGAATCA GGAGAAGCAG 
GAGGTGTGGC GGGAGGCCCG CCTTAAATTC CCAAATCTCA ATCATCGGGA TATTTGGTCA 
ATGTTTAAGT TCGCCTTCCG CACATACCTG GATGATTTAG AGCGCGGCAT CGATAATCCC 
TGGCCGCAGA ATTGGTGGCA GGCACTGGAG CAACTCAGGT TTCTGGTCAA TGTGCGCTAC 
CATCCGCTCG AGCCCTACTA CTATATAGTG CACAACAGGA TGTCAGAGGA GGTGAAGCGC 
TGCAAAATGC ACGAGGCTCT AACGTCCTCC GATCATATCG ATAAGACCAA ATTTACACCG 
AGCAAACTTT TAACGGGCTT TTCCAAAGAA CCCAAGCCAT GGGAGACGGA GGAGGCCAAG 
CGCTTGCTTA CCGGCAAGTT GAATAGCGTG AGAAGCACTG AAACTCTCAC TCAAATTGCT 
CCAGCTCCGG CTCTAGCTTT GGCACCCGAT GCCCAACCTA TTCCTGTACC TGATGGCGTT 
TCCGTGTCCA AAAAGACAAG CGAAAGCAAG CCAGCGTCAA ACGCCAACGT CAAGTCTCAT 
AAAGACAAGA ATATCTCCAC CGCCCAAAAG CGGCCGACCC CAGCGAGAAG TGTCACCTGT 
AACAATCTAT CCCCCATTGA GGCGTTTGAG TTGTGTAAGA AATTGCGTAG CCAACCAAAC 
AGCTTCGAGC GGGCGAGCAC TCTGGACAAG CGAGCCGCCT GGGTTCGCGT ATCAAAGGAG 
TTGCATGCCA CAGTGACCGA ATGCCGCCTT GGTCTGCAGT ACGCTCTGCG CGAGATGCGC 
AATCTGAAGA TCGCCGATCC AAGCAATCAA TGTACAATGG GCCACAAGTA CTTGCACCAC 
ATGTCGGAAA TCTATAAGCA GGTGAAGCCG AACGCCCAGC TGACAGTCCG CACGCCACAG 
CAGCTCAACC AGTTAAACGA ATCGCTTGCG AAGAACACTC AGGAAGTTAG CCCGCAGGAG 
TTCCTGCCTG AGATCAATTT GTCCACTTGC AGTCCGGATC TCGTGGTGAA GAATTGGGCC 
TACGCCGTCG GTAACTTGTC CACGGCCAGT CAGGATACAC TGCTCGGCAA GATGACGCAG 
CTGTTCTCTA AATACGCCAA GGTCAATCCG CCACCGCCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hmr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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