spirit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spirit
Entrez Gene ID	31797
Full Name	Serine Protease Immune Response Integrator
Synonyms	CG2056,Dmel\CG2056,SP33,Spirit,c-SP33
General protein information	Preferred Names Serine Protease Immune Response Integrator Names CG2056 gene product from transcript CG2056-RC CG2056-PA CG2056-PB CG2056-PC CG2056-PD serine protease immune response integrator spirit-PA spirit-PB spirit-PC spirit-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7F5-7F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_132264.3	NP_572492.1	serine protease immune response integrator, isoform A
NM_167161.2	NP_727276.1	serine protease immune response integrator, isoform B
NM_001169236.2	NP_001162707.1	serine protease immune response integrator, isoform C
NM_001272413.1	NP_001259342.1	serine protease immune response integrator, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG11668	NP_650254.1
Drosophila melanogaster (fruit fly)	spirit	NP_727276.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spirit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spirit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01136	NM_132264.3 Latest version!	Drosophila melanogaster serine protease immune response integrator, transcript variant A (spirit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01136	NM_001272413.1 Latest version!	Drosophila melanogaster serine protease immune response integrator, transcript variant D (spirit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01136	NM_167161.2 Latest version!	Drosophila melanogaster serine protease immune response integrator, transcript variant B (spirit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01136	NM_001169236.2 Latest version!	Drosophila melanogaster serine protease immune response integrator, transcript variant C (spirit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spirit ( NM_132264.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572492.1 Drosophila melanogaster serine protease immune response integrator, transcript variant A (spirit), mRNA.

Price & Availability↑

CloneID	OFa01136
Clone ID Related Accession (Same CDS sequence)	NM_132264.3 , NM_167161.2 , NM_001169236.2 , NM_001272413.1
Accession Version	NM_132264.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease immune response integrator, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Dec 17, 2009 this sequence version replaced NM_132264.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572492.1
CDS	155..1336
Translation	MSIRSTHCIPTRVYLHLLLVSLPLLAVHATPAISPQSLRGIIFPVETFDECQLEDVARTKGTCRRMEDCPSALNGWLERRESPKTCYFVRFDHYVCCAPAVAPIVTRSSQQACNELNKVSKVKEIDEFFVSVVGGMPTRPREFPFMAALGWRSNFDQRIYYRCGGALIANNFVLTAAHCADLGGEPPSQVRLGGDNLTLTEGEDISIRRVIIHPDYSASTAYNDIALLELETAAKPELKPTCIWTQKEVTNTLVTAIGYGQTSFAGLSSAQLLKVPLKSVSNEECQHHYQKDQLAQGVLGTQMCAGDITGERDTCQGDSGGPLLMQDGLLGYVVGITSLGQGCASGPPSVYTRVSSFVDWIEGIVWPAQQVTNAPQPNQMTSFSPEFDLRATI

Target ORF information:

RefSeq Version	NM_132264.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease immune response integrator, transcript variant A (spirit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132264.3

ORF Insert Sequence:

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spirit ( NM_167161.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727276.1 Drosophila melanogaster serine protease immune response integrator, transcript variant B (spirit), mRNA.

Price & Availability↑

CloneID	OFa01136
Clone ID Related Accession (Same CDS sequence)	NM_132264.3 , NM_167161.2 , NM_001169236.2 , NM_001272413.1
Accession Version	NM_167161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease immune response integrator, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167161.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727276.1
CDS	159..1340
Translation	MSIRSTHCIPTRVYLHLLLVSLPLLAVHATPAISPQSLRGIIFPVETFDECQLEDVARTKGTCRRMEDCPSALNGWLERRESPKTCYFVRFDHYVCCAPAVAPIVTRSSQQACNELNKVSKVKEIDEFFVSVVGGMPTRPREFPFMAALGWRSNFDQRIYYRCGGALIANNFVLTAAHCADLGGEPPSQVRLGGDNLTLTEGEDISIRRVIIHPDYSASTAYNDIALLELETAAKPELKPTCIWTQKEVTNTLVTAIGYGQTSFAGLSSAQLLKVPLKSVSNEECQHHYQKDQLAQGVLGTQMCAGDITGERDTCQGDSGGPLLMQDGLLGYVVGITSLGQGCASGPPSVYTRVSSFVDWIEGIVWPAQQVTNAPQPNQMTSFSPEFDLRATI

Target ORF information:

RefSeq Version	NM_167161.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease immune response integrator, transcript variant B (spirit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167161.2

ORF Insert Sequence:

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spirit ( NM_001169236.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162707.1 Drosophila melanogaster serine protease immune response integrator, transcript variant C (spirit), mRNA.

Price & Availability↑

CloneID	OFa01136
Clone ID Related Accession (Same CDS sequence)	NM_132264.3 , NM_167161.2 , NM_001169236.2 , NM_001272413.1
Accession Version	NM_001169236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease immune response integrator, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169236.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162707.1
CDS	126..1307
Translation	MSIRSTHCIPTRVYLHLLLVSLPLLAVHATPAISPQSLRGIIFPVETFDECQLEDVARTKGTCRRMEDCPSALNGWLERRESPKTCYFVRFDHYVCCAPAVAPIVTRSSQQACNELNKVSKVKEIDEFFVSVVGGMPTRPREFPFMAALGWRSNFDQRIYYRCGGALIANNFVLTAAHCADLGGEPPSQVRLGGDNLTLTEGEDISIRRVIIHPDYSASTAYNDIALLELETAAKPELKPTCIWTQKEVTNTLVTAIGYGQTSFAGLSSAQLLKVPLKSVSNEECQHHYQKDQLAQGVLGTQMCAGDITGERDTCQGDSGGPLLMQDGLLGYVVGITSLGQGCASGPPSVYTRVSSFVDWIEGIVWPAQQVTNAPQPNQMTSFSPEFDLRATI

Target ORF information:

RefSeq Version	NM_001169236.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease immune response integrator, transcript variant C (spirit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169236.2

ORF Insert Sequence:

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spirit ( NM_001272413.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259342.1 Drosophila melanogaster serine protease immune response integrator, transcript variant D (spirit), mRNA.

Price & Availability↑

CloneID	OFa01136
Clone ID Related Accession (Same CDS sequence)	NM_132264.3 , NM_167161.2 , NM_001169236.2 , NM_001272413.1
Accession Version	NM_001272413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serine protease immune response integrator, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259342.1
CDS	175..1356
Translation	MSIRSTHCIPTRVYLHLLLVSLPLLAVHATPAISPQSLRGIIFPVETFDECQLEDVARTKGTCRRMEDCPSALNGWLERRESPKTCYFVRFDHYVCCAPAVAPIVTRSSQQACNELNKVSKVKEIDEFFVSVVGGMPTRPREFPFMAALGWRSNFDQRIYYRCGGALIANNFVLTAAHCADLGGEPPSQVRLGGDNLTLTEGEDISIRRVIIHPDYSASTAYNDIALLELETAAKPELKPTCIWTQKEVTNTLVTAIGYGQTSFAGLSSAQLLKVPLKSVSNEECQHHYQKDQLAQGVLGTQMCAGDITGERDTCQGDSGGPLLMQDGLLGYVVGITSLGQGCASGPPSVYTRVSSFVDWIEGIVWPAQQVTNAPQPNQMTSFSPEFDLRATI

Target ORF information:

RefSeq Version	NM_001272413.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serine protease immune response integrator, transcript variant D (spirit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272413.1

ORF Insert Sequence:

ATGAGCATTC GGTCTACCCA TTGCATACCC ACTCGTGTGT ATCTGCACCT TCTGCTGGTG 
TCGCTACCAC TTTTGGCAGT TCACGCCACG CCCGCCATAA GTCCGCAATC GTTGCGCGGC 
ATCATCTTCC CCGTCGAAAC CTTCGATGAG TGCCAACTGG AGGACGTGGC GCGGACGAAG 
GGCACCTGCC GGCGCATGGA GGACTGTCCC AGTGCCCTGA ACGGCTGGCT AGAGCGGCGC 
GAATCGCCCA AGACCTGCTA CTTTGTGAGG TTCGACCACT ATGTGTGCTG CGCCCCAGCT 
GTAGCTCCAA TTGTCACCCG ATCCAGCCAG CAGGCCTGCA ATGAACTGAA CAAGGTGTCA 
AAGGTCAAAG AGATCGACGA GTTCTTCGTG TCGGTGGTGG GCGGCATGCC CACCCGTCCG 
CGGGAGTTCC CCTTCATGGC CGCATTGGGC TGGCGTTCGA ACTTCGATCA GAGGATATAC 
TATCGATGCG GCGGCGCCCT TATCGCCAAC AACTTTGTCC TGACCGCAGC CCACTGTGCC 
GATTTGGGCG GTGAGCCACC CAGTCAGGTG CGCCTCGGTG GCGACAATCT AACCCTGACC 
GAGGGCGAGG ATATAAGCAT TCGGCGCGTG ATCATCCATC CGGATTACAG TGCCAGCACG 
GCATACAACG ATATTGCGCT GCTTGAGCTG GAGACGGCGG CCAAGCCGGA ACTGAAGCCC 
ACTTGCATTT GGACGCAGAA GGAGGTGACC AACACGCTGG TCACGGCCAT TGGTTATGGG 
CAGACCAGCT TCGCCGGTCT ATCCTCCGCT CAGCTGCTAA AGGTGCCGCT GAAAAGCGTG 
AGCAACGAGG AGTGCCAGCA CCACTACCAA AAGGATCAGC TGGCTCAGGG CGTGCTGGGC 
ACGCAGATGT GCGCCGGCGA TATAACCGGC GAGCGGGACA CCTGCCAGGG CGATTCCGGC 
GGACCGCTGC TCATGCAGGA CGGCCTGCTT GGCTATGTGG TGGGCATCAC ATCGCTGGGA 
CAGGGATGCG CCAGTGGACC GCCATCGGTG TACACCAGGG TGTCCAGCTT CGTCGACTGG 
ATCGAGGGCA TTGTGTGGCC GGCGCAGCAG GTGACGAACG CACCGCAACC CAACCAGATG 
ACCAGTTTCA GTCCCGAATT CGATCTGCGG GCGACGATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spirit cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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