mahe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mahe
Entrez Gene ID	31707
Full Name	maheshvara
Synonyms	CG10777,DmRH1,Dmel\CG10777,cg10777
General protein information	Preferred Names maheshvara Names CG10777 gene product from transcript CG10777-RB CG10777-PB CG10777-PC mahe-PB mahe-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7C3-7C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298065.1	NP_001284994.1	maheshvara, isoform C
NM_132196.3	NP_572424.1	maheshvara, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mahe, which was originally designated as CG10777 in FlyBase, shows a dynamic pattern of expression and is expressed maternally and exhibits strong neuronal expression during embryogenesis and larval development
Title: Regulation of Notch Signaling by an Evolutionary Conserved DEAD Box RNA Helicase, Maheshvara in Drosophila melanogaster.

The following mahe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mahe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01054	NM_132196.3 Latest version!	Drosophila melanogaster maheshvara (mahe), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01054	NM_001298065.1 Latest version!	Drosophila melanogaster maheshvara (mahe), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mahe ( NM_001298065.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284994.1 Drosophila melanogaster maheshvara (mahe), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01054
Clone ID Related Accession (Same CDS sequence)	NM_001298065.1 , NM_132196.3
Accession Version	NM_001298065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maheshvara, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGCTG GAGCATATGC ACCCAACGCT GGGGGCGTTC AGCAGCACAC TGGCGGCGGT 
TACTATGGCA ATGGTGCCGC AGCAGGCGCT GGCGGTAGCG GCAACGGGGC CCCACGCCAC 
GAGCGTCAAT ACTATCCGCG CGGCAATTTC GGCGGTGCCG CCGGGGGAGC TGGCATCAAT 
GGAGCAGCCA CTGCGGCTGG AAGCATGCAC TTTGGTCAGC CCTATGGCGT GATGACCTAT 
GGCATCCAGA ATGGTCTGGG AATGGGAGCC GCCGGTTCTG GTGGCGGCAA TCCGTATCGC 
CAGCAAAACG GTGTTGCATT CGCCGGTAAT GGACTTGGAG GAGGCGCCGG TGGCGGATCC 
GGAGGATCTG GCGGATATGG AGGACAGCGT GGCAAAAATC CAACCTACGC ACAGCGTTAT 
CAGAAGCCGA ACAACGGCGC CGGAGTTGCT GGTGGCTACC AAAGCAATAA CTACAATGCT 
GCCGCCTTAG GCATGCTTTC CAAAGAGGAG CGCGCCGAGA TCCAACGTGA AAAGGCCAAA 
AATCCCGGCC GAAATTTAGT GAAACCCAAG TGGGAGAACC TCGAGCCCTT CCTCAAAGAT 
TTCTACAACA TTCATCCGAA TACACTGGCC AAATCAGAGC AGCAGGTGGC CGAGATCCGG 
CGCGAACTGG AAATCACCGT GTCTGGCAAT GAACTACCAC ACCCGGTGGT CAGTTTCGAG 
GAGAGCTCGC TGCCCGCTCA CGTCATTGAA GAGATGAAGC GCCAGGGATT CACCAAGCCC 
ACTGCTATCC AATCGCAGGG TTGGCCCATT GCCTTAAGTG GCCGCGATTT GGTGGGCATT 
GCCCAGACCG GTTCAGGCAA GACGCTGGCA TACATGCTGC CTGCCATCGT GCACATTGGC 
AATCAGCCGC CAATAATTCG CGGCGAAGGA CCCATTGCTC TAGTGTTGGC CCCCACCCGG 
GAGCTGGCCC AGCAGATCCA ATCTGTCGTG CGAGACTATG GACATCTGTG CAAGCCAGAG 
ATTCGTCACA CTTGCATCTT TGGCGGCTCA TCGAAGGTGC CGCAGGCACG CGATCTTGAC 
CGCGGCGTCG AGGTGATCAT CGCCACACCA GGACGTCTGA TTGATTTCCT TGAGAATCGC 
AACACTAACT TGCAGAGATG CACTTATCTG GTGCTGGACG AAGCCGACCG CATGCTGGAC 
ATGGGTTTCG AGCCGCAGAT CCGTAAGATC ATTGAACAGA TTCGTCCCGA CCGTCAGGTG 
GTCATGTGGT CCGCAACCTG GCCAAAGGAG GTGCAGGCCC TAGCCGGAGA CTTTTTAAAC 
GATTATATCC AAATCAACAT TGGTTCGATG AACCTATCGG CTAACCACAA CATTCGCCAG 
ATTGTCGAGA TCTGTACAGA AATAGAGAAG CCGCAGCGCT TAGTATGCTT GCTCAATGAG 
ATTTCTCCCA TCAAGAATTC GGGAAACAAT GGGAACAAAA TTATCGTCTT TGTGGAGACC 
AAGATCAAGG TGGAGGACAT CTTGCAGATC ATTCGAGCCG AGGGTTACAA CGCCACCTCA 
ATTCACGGCG ACAAGACCCA GAATGAGCGT GATTCAGTGC TAAAGGATTT CCGTAACGGC 
AAGTCCAACA TTCTGATTGC CACCGATGTG GCTTCGCGCG GCCTCGATGT CGAGGATCTG 
CAGTACGTAA TTAACTATGA TTACCCGAAC TCCTCGGAGA ACTACGTGCA CCGTATCGGG 
CGCACTGGTC GCTGCCAGCA GCTGGGAACT GCCTACACAT TTTTCACGCC TGACAATGCC 
AAGCAGGCCC GTGAACTGAT CTCTGTGCTC GAGGAGGCTG GCCAGACGCC GTCGCAGGCT 
CTTTTGGATC TTGCTCGTTC GATGCCCAGC TCGGGCGGCT ATCGCGGCAA CAAGCGCTGG 
AACAACAACA GCGGCGGGGA TCGCAACACT GGCGGTAACA ACGGCATCTA TCAGCAGCGC 
AACAACAATC CGCTGAATTA CCAGGCCGGT AACAACTATA ACAACAATCG CAGCGGCCCC 
CCAACTGGCA GTAGCTACCA GCAGTATGCC GCTGGCGGCA ATACCTATCT GCAGAATGGT 
GCTGGTGGTG GCGTTAATTG GAATCGCATG AACCAGATGG GACAGGATGG AGGAGCCGGA 
CAACAGCAGC GAAACGGCAA CACCTATCGC CCGAGGGCTC CGTTCAACAA TGGCGGACCA 
CGCGCCATTA TGGGCCATCA GGGTGGCGGC GTCGGAGCTG GTGGAGCAGC CGGCGTAGGC 
GGTGGCCCAC AGCAGCTCAT GGCACCCCAG CAGGGCGGAC AGTATATGCC ACAAGCATAC 
CGCGGACGTA GCCAGTTTGT GCCGCAGGGA GCTGGTGCCG GTGGCATGCC GCCACGATTC 
CAGCAGTATC CCAAGCGGGA GTACCAGCAA CAAGGAGTAA CCCACTACGG TCAGCAGGCT 
TTGCAGCATC AGCAACAACA GCAGCAGGCT GGCCAACAGA CCAAGCCGCC CAAGGACGAA 
AGCAACAAGT ATGTTCCGTC GGCTGCGGTG ACCAGCTCGC TGGTCCAATA CACGCCAGCC 
AATTCGCCAA CGATTGCGGC CGTAGCTGCT GCCGCTGCAG CAGCCGCCGG ACGAGCTGCA 
GCTGCAGCTG TGGCTCCGGC TCCCGGTGCC GCCTATATGT ACGATGCGGC CGGTGTTCTC 
ACTACCGCCG CTGCGCCGGG CACCAATCCA TATGCCAACC AGTTCAGCAT GCCGGCCACC 
TACTATGCCG CCCAACATCA CCAGTTCGCC CAAGCCGTGG CAGGACCCGG ACCAGCACCC 
GGCCAGGCCA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284994.1
CDS	523..3360
Translation	MFAGAYAPNAGGVQQHTGGGYYGNGAAAGAGGSGNGAPRHERQYYPRGNFGGAAGGAGINGAATAAGSMHFGQPYGVMTYGIQNGLGMGAAGSGGGNPYRQQNGVAFAGNGLGGGAGGGSGGSGGYGGQRGKNPTYAQRYQKPNNGAGVAGGYQSNNYNAAALGMLSKEERAEIQREKAKNPGRNLVKPKWENLEPFLKDFYNIHPNTLAKSEQQVAEIRRELEITVSGNELPHPVVSFEESSLPAHVIEEMKRQGFTKPTAIQSQGWPIALSGRDLVGIAQTGSGKTLAYMLPAIVHIGNQPPIIRGEGPIALVLAPTRELAQQIQSVVRDYGHLCKPEIRHTCIFGGSSKVPQARDLDRGVEVIIATPGRLIDFLENRNTNLQRCTYLVLDEADRMLDMGFEPQIRKIIEQIRPDRQVVMWSATWPKEVQALAGDFLNDYIQINIGSMNLSANHNIRQIVEICTEIEKPQRLVCLLNEISPIKNSGNNGNKIIVFVETKIKVEDILQIIRAEGYNATSIHGDKTQNERDSVLKDFRNGKSNILIATDVASRGLDVEDLQYVINYDYPNSSENYVHRIGRTGRCQQLGTAYTFFTPDNAKQARELISVLEEAGQTPSQALLDLARSMPSSGGYRGNKRWNNNSGGDRNTGGNNGIYQQRNNNPLNYQAGNNYNNNRSGPPTGSSYQQYAAGGNTYLQNGAGGGVNWNRMNQMGQDGGAGQQQRNGNTYRPRAPFNNGGPRAIMGHQGGGVGAGGAAGVGGGPQQLMAPQQGGQYMPQAYRGRSQFVPQGAGAGGMPPRFQQYPKREYQQQGVTHYGQQALQHQQQQQQAGQQTKPPKDESNKYVPSAAVTSSLVQYTPANSPTIAAVAAAAAAAAGRAAAAAVAPAPGAAYMYDAAGVLTTAAAPGTNPYANQFSMPATYYAAQHHQFAQAVAGPGPAPGQAIE

Target ORF information:

RefSeq Version	NM_001298065.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maheshvara (mahe), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298065.1

ORF Insert Sequence:

ATGTTCGCTG GAGCATATGC ACCCAACGCT GGGGGCGTTC AGCAGCACAC TGGCGGCGGT 
TACTATGGCA ATGGTGCCGC AGCAGGCGCT GGCGGTAGCG GCAACGGGGC CCCACGCCAC 
GAGCGTCAAT ACTATCCGCG CGGCAATTTC GGCGGTGCCG CCGGGGGAGC TGGCATCAAT 
GGAGCAGCCA CTGCGGCTGG AAGCATGCAC TTTGGTCAGC CCTATGGCGT GATGACCTAT 
GGCATCCAGA ATGGTCTGGG AATGGGAGCC GCCGGTTCTG GTGGCGGCAA TCCGTATCGC 
CAGCAAAACG GTGTTGCATT CGCCGGTAAT GGACTTGGAG GAGGCGCCGG TGGCGGATCC 
GGAGGATCTG GCGGATATGG AGGACAGCGT GGCAAAAATC CAACCTACGC ACAGCGTTAT 
CAGAAGCCGA ACAACGGCGC CGGAGTTGCT GGTGGCTACC AAAGCAATAA CTACAATGCT 
GCCGCCTTAG GCATGCTTTC CAAAGAGGAG CGCGCCGAGA TCCAACGTGA AAAGGCCAAA 
AATCCCGGCC GAAATTTAGT GAAACCCAAG TGGGAGAACC TCGAGCCCTT CCTCAAAGAT 
TTCTACAACA TTCATCCGAA TACACTGGCC AAATCAGAGC AGCAGGTGGC CGAGATCCGG 
CGCGAACTGG AAATCACCGT GTCTGGCAAT GAACTACCAC ACCCGGTGGT CAGTTTCGAG 
GAGAGCTCGC TGCCCGCTCA CGTCATTGAA GAGATGAAGC GCCAGGGATT CACCAAGCCC 
ACTGCTATCC AATCGCAGGG TTGGCCCATT GCCTTAAGTG GCCGCGATTT GGTGGGCATT 
GCCCAGACCG GTTCAGGCAA GACGCTGGCA TACATGCTGC CTGCCATCGT GCACATTGGC 
AATCAGCCGC CAATAATTCG CGGCGAAGGA CCCATTGCTC TAGTGTTGGC CCCCACCCGG 
GAGCTGGCCC AGCAGATCCA ATCTGTCGTG CGAGACTATG GACATCTGTG CAAGCCAGAG 
ATTCGTCACA CTTGCATCTT TGGCGGCTCA TCGAAGGTGC CGCAGGCACG CGATCTTGAC 
CGCGGCGTCG AGGTGATCAT CGCCACACCA GGACGTCTGA TTGATTTCCT TGAGAATCGC 
AACACTAACT TGCAGAGATG CACTTATCTG GTGCTGGACG AAGCCGACCG CATGCTGGAC 
ATGGGTTTCG AGCCGCAGAT CCGTAAGATC ATTGAACAGA TTCGTCCCGA CCGTCAGGTG 
GTCATGTGGT CCGCAACCTG GCCAAAGGAG GTGCAGGCCC TAGCCGGAGA CTTTTTAAAC 
GATTATATCC AAATCAACAT TGGTTCGATG AACCTATCGG CTAACCACAA CATTCGCCAG 
ATTGTCGAGA TCTGTACAGA AATAGAGAAG CCGCAGCGCT TAGTATGCTT GCTCAATGAG 
ATTTCTCCCA TCAAGAATTC GGGAAACAAT GGGAACAAAA TTATCGTCTT TGTGGAGACC 
AAGATCAAGG TGGAGGACAT CTTGCAGATC ATTCGAGCCG AGGGTTACAA CGCCACCTCA 
ATTCACGGCG ACAAGACCCA GAATGAGCGT GATTCAGTGC TAAAGGATTT CCGTAACGGC 
AAGTCCAACA TTCTGATTGC CACCGATGTG GCTTCGCGCG GCCTCGATGT CGAGGATCTG 
CAGTACGTAA TTAACTATGA TTACCCGAAC TCCTCGGAGA ACTACGTGCA CCGTATCGGG 
CGCACTGGTC GCTGCCAGCA GCTGGGAACT GCCTACACAT TTTTCACGCC TGACAATGCC 
AAGCAGGCCC GTGAACTGAT CTCTGTGCTC GAGGAGGCTG GCCAGACGCC GTCGCAGGCT 
CTTTTGGATC TTGCTCGTTC GATGCCCAGC TCGGGCGGCT ATCGCGGCAA CAAGCGCTGG 
AACAACAACA GCGGCGGGGA TCGCAACACT GGCGGTAACA ACGGCATCTA TCAGCAGCGC 
AACAACAATC CGCTGAATTA CCAGGCCGGT AACAACTATA ACAACAATCG CAGCGGCCCC 
CCAACTGGCA GTAGCTACCA GCAGTATGCC GCTGGCGGCA ATACCTATCT GCAGAATGGT 
GCTGGTGGTG GCGTTAATTG GAATCGCATG AACCAGATGG GACAGGATGG AGGAGCCGGA 
CAACAGCAGC GAAACGGCAA CACCTATCGC CCGAGGGCTC CGTTCAACAA TGGCGGACCA 
CGCGCCATTA TGGGCCATCA GGGTGGCGGC GTCGGAGCTG GTGGAGCAGC CGGCGTAGGC 
GGTGGCCCAC AGCAGCTCAT GGCACCCCAG CAGGGCGGAC AGTATATGCC ACAAGCATAC 
CGCGGACGTA GCCAGTTTGT GCCGCAGGGA GCTGGTGCCG GTGGCATGCC GCCACGATTC 
CAGCAGTATC CCAAGCGGGA GTACCAGCAA CAAGGAGTAA CCCACTACGG TCAGCAGGCT 
TTGCAGCATC AGCAACAACA GCAGCAGGCT GGCCAACAGA CCAAGCCGCC CAAGGACGAA 
AGCAACAAGT ATGTTCCGTC GGCTGCGGTG ACCAGCTCGC TGGTCCAATA CACGCCAGCC 
AATTCGCCAA CGATTGCGGC CGTAGCTGCT GCCGCTGCAG CAGCCGCCGG ACGAGCTGCA 
GCTGCAGCTG TGGCTCCGGC TCCCGGTGCC GCCTATATGT ACGATGCGGC CGGTGTTCTC 
ACTACCGCCG CTGCGCCGGG CACCAATCCA TATGCCAACC AGTTCAGCAT GCCGGCCACC 
TACTATGCCG CCCAACATCA CCAGTTCGCC CAAGCCGTGG CAGGACCCGG ACCAGCACCC 
GGCCAGGCCA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mahe ( NM_132196.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572424.1 Drosophila melanogaster maheshvara (mahe), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01054
Clone ID Related Accession (Same CDS sequence)	NM_001298065.1 , NM_132196.3
Accession Version	NM_132196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	maheshvara, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132196.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGCTG GAGCATATGC ACCCAACGCT GGGGGCGTTC AGCAGCACAC TGGCGGCGGT 
TACTATGGCA ATGGTGCCGC AGCAGGCGCT GGCGGTAGCG GCAACGGGGC CCCACGCCAC 
GAGCGTCAAT ACTATCCGCG CGGCAATTTC GGCGGTGCCG CCGGGGGAGC TGGCATCAAT 
GGAGCAGCCA CTGCGGCTGG AAGCATGCAC TTTGGTCAGC CCTATGGCGT GATGACCTAT 
GGCATCCAGA ATGGTCTGGG AATGGGAGCC GCCGGTTCTG GTGGCGGCAA TCCGTATCGC 
CAGCAAAACG GTGTTGCATT CGCCGGTAAT GGACTTGGAG GAGGCGCCGG TGGCGGATCC 
GGAGGATCTG GCGGATATGG AGGACAGCGT GGCAAAAATC CAACCTACGC ACAGCGTTAT 
CAGAAGCCGA ACAACGGCGC CGGAGTTGCT GGTGGCTACC AAAGCAATAA CTACAATGCT 
GCCGCCTTAG GCATGCTTTC CAAAGAGGAG CGCGCCGAGA TCCAACGTGA AAAGGCCAAA 
AATCCCGGCC GAAATTTAGT GAAACCCAAG TGGGAGAACC TCGAGCCCTT CCTCAAAGAT 
TTCTACAACA TTCATCCGAA TACACTGGCC AAATCAGAGC AGCAGGTGGC CGAGATCCGG 
CGCGAACTGG AAATCACCGT GTCTGGCAAT GAACTACCAC ACCCGGTGGT CAGTTTCGAG 
GAGAGCTCGC TGCCCGCTCA CGTCATTGAA GAGATGAAGC GCCAGGGATT CACCAAGCCC 
ACTGCTATCC AATCGCAGGG TTGGCCCATT GCCTTAAGTG GCCGCGATTT GGTGGGCATT 
GCCCAGACCG GTTCAGGCAA GACGCTGGCA TACATGCTGC CTGCCATCGT GCACATTGGC 
AATCAGCCGC CAATAATTCG CGGCGAAGGA CCCATTGCTC TAGTGTTGGC CCCCACCCGG 
GAGCTGGCCC AGCAGATCCA ATCTGTCGTG CGAGACTATG GACATCTGTG CAAGCCAGAG 
ATTCGTCACA CTTGCATCTT TGGCGGCTCA TCGAAGGTGC CGCAGGCACG CGATCTTGAC 
CGCGGCGTCG AGGTGATCAT CGCCACACCA GGACGTCTGA TTGATTTCCT TGAGAATCGC 
AACACTAACT TGCAGAGATG CACTTATCTG GTGCTGGACG AAGCCGACCG CATGCTGGAC 
ATGGGTTTCG AGCCGCAGAT CCGTAAGATC ATTGAACAGA TTCGTCCCGA CCGTCAGGTG 
GTCATGTGGT CCGCAACCTG GCCAAAGGAG GTGCAGGCCC TAGCCGGAGA CTTTTTAAAC 
GATTATATCC AAATCAACAT TGGTTCGATG AACCTATCGG CTAACCACAA CATTCGCCAG 
ATTGTCGAGA TCTGTACAGA AATAGAGAAG CCGCAGCGCT TAGTATGCTT GCTCAATGAG 
ATTTCTCCCA TCAAGAATTC GGGAAACAAT GGGAACAAAA TTATCGTCTT TGTGGAGACC 
AAGATCAAGG TGGAGGACAT CTTGCAGATC ATTCGAGCCG AGGGTTACAA CGCCACCTCA 
ATTCACGGCG ACAAGACCCA GAATGAGCGT GATTCAGTGC TAAAGGATTT CCGTAACGGC 
AAGTCCAACA TTCTGATTGC CACCGATGTG GCTTCGCGCG GCCTCGATGT CGAGGATCTG 
CAGTACGTAA TTAACTATGA TTACCCGAAC TCCTCGGAGA ACTACGTGCA CCGTATCGGG 
CGCACTGGTC GCTGCCAGCA GCTGGGAACT GCCTACACAT TTTTCACGCC TGACAATGCC 
AAGCAGGCCC GTGAACTGAT CTCTGTGCTC GAGGAGGCTG GCCAGACGCC GTCGCAGGCT 
CTTTTGGATC TTGCTCGTTC GATGCCCAGC TCGGGCGGCT ATCGCGGCAA CAAGCGCTGG 
AACAACAACA GCGGCGGGGA TCGCAACACT GGCGGTAACA ACGGCATCTA TCAGCAGCGC 
AACAACAATC CGCTGAATTA CCAGGCCGGT AACAACTATA ACAACAATCG CAGCGGCCCC 
CCAACTGGCA GTAGCTACCA GCAGTATGCC GCTGGCGGCA ATACCTATCT GCAGAATGGT 
GCTGGTGGTG GCGTTAATTG GAATCGCATG AACCAGATGG GACAGGATGG AGGAGCCGGA 
CAACAGCAGC GAAACGGCAA CACCTATCGC CCGAGGGCTC CGTTCAACAA TGGCGGACCA 
CGCGCCATTA TGGGCCATCA GGGTGGCGGC GTCGGAGCTG GTGGAGCAGC CGGCGTAGGC 
GGTGGCCCAC AGCAGCTCAT GGCACCCCAG CAGGGCGGAC AGTATATGCC ACAAGCATAC 
CGCGGACGTA GCCAGTTTGT GCCGCAGGGA GCTGGTGCCG GTGGCATGCC GCCACGATTC 
CAGCAGTATC CCAAGCGGGA GTACCAGCAA CAAGGAGTAA CCCACTACGG TCAGCAGGCT 
TTGCAGCATC AGCAACAACA GCAGCAGGCT GGCCAACAGA CCAAGCCGCC CAAGGACGAA 
AGCAACAAGT ATGTTCCGTC GGCTGCGGTG ACCAGCTCGC TGGTCCAATA CACGCCAGCC 
AATTCGCCAA CGATTGCGGC CGTAGCTGCT GCCGCTGCAG CAGCCGCCGG ACGAGCTGCA 
GCTGCAGCTG TGGCTCCGGC TCCCGGTGCC GCCTATATGT ACGATGCGGC CGGTGTTCTC 
ACTACCGCCG CTGCGCCGGG CACCAATCCA TATGCCAACC AGTTCAGCAT GCCGGCCACC 
TACTATGCCG CCCAACATCA CCAGTTCGCC CAAGCCGTGG CAGGACCCGG ACCAGCACCC 
GGCCAGGCCA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572424.1
CDS	523..3360
Translation	MFAGAYAPNAGGVQQHTGGGYYGNGAAAGAGGSGNGAPRHERQYYPRGNFGGAAGGAGINGAATAAGSMHFGQPYGVMTYGIQNGLGMGAAGSGGGNPYRQQNGVAFAGNGLGGGAGGGSGGSGGYGGQRGKNPTYAQRYQKPNNGAGVAGGYQSNNYNAAALGMLSKEERAEIQREKAKNPGRNLVKPKWENLEPFLKDFYNIHPNTLAKSEQQVAEIRRELEITVSGNELPHPVVSFEESSLPAHVIEEMKRQGFTKPTAIQSQGWPIALSGRDLVGIAQTGSGKTLAYMLPAIVHIGNQPPIIRGEGPIALVLAPTRELAQQIQSVVRDYGHLCKPEIRHTCIFGGSSKVPQARDLDRGVEVIIATPGRLIDFLENRNTNLQRCTYLVLDEADRMLDMGFEPQIRKIIEQIRPDRQVVMWSATWPKEVQALAGDFLNDYIQINIGSMNLSANHNIRQIVEICTEIEKPQRLVCLLNEISPIKNSGNNGNKIIVFVETKIKVEDILQIIRAEGYNATSIHGDKTQNERDSVLKDFRNGKSNILIATDVASRGLDVEDLQYVINYDYPNSSENYVHRIGRTGRCQQLGTAYTFFTPDNAKQARELISVLEEAGQTPSQALLDLARSMPSSGGYRGNKRWNNNSGGDRNTGGNNGIYQQRNNNPLNYQAGNNYNNNRSGPPTGSSYQQYAAGGNTYLQNGAGGGVNWNRMNQMGQDGGAGQQQRNGNTYRPRAPFNNGGPRAIMGHQGGGVGAGGAAGVGGGPQQLMAPQQGGQYMPQAYRGRSQFVPQGAGAGGMPPRFQQYPKREYQQQGVTHYGQQALQHQQQQQQAGQQTKPPKDESNKYVPSAAVTSSLVQYTPANSPTIAAVAAAAAAAAGRAAAAAVAPAPGAAYMYDAAGVLTTAAAPGTNPYANQFSMPATYYAAQHHQFAQAVAGPGPAPGQAIE

Target ORF information:

RefSeq Version	NM_132196.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster maheshvara (mahe), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132196.3

ORF Insert Sequence:

ATGTTCGCTG GAGCATATGC ACCCAACGCT GGGGGCGTTC AGCAGCACAC TGGCGGCGGT 
TACTATGGCA ATGGTGCCGC AGCAGGCGCT GGCGGTAGCG GCAACGGGGC CCCACGCCAC 
GAGCGTCAAT ACTATCCGCG CGGCAATTTC GGCGGTGCCG CCGGGGGAGC TGGCATCAAT 
GGAGCAGCCA CTGCGGCTGG AAGCATGCAC TTTGGTCAGC CCTATGGCGT GATGACCTAT 
GGCATCCAGA ATGGTCTGGG AATGGGAGCC GCCGGTTCTG GTGGCGGCAA TCCGTATCGC 
CAGCAAAACG GTGTTGCATT CGCCGGTAAT GGACTTGGAG GAGGCGCCGG TGGCGGATCC 
GGAGGATCTG GCGGATATGG AGGACAGCGT GGCAAAAATC CAACCTACGC ACAGCGTTAT 
CAGAAGCCGA ACAACGGCGC CGGAGTTGCT GGTGGCTACC AAAGCAATAA CTACAATGCT 
GCCGCCTTAG GCATGCTTTC CAAAGAGGAG CGCGCCGAGA TCCAACGTGA AAAGGCCAAA 
AATCCCGGCC GAAATTTAGT GAAACCCAAG TGGGAGAACC TCGAGCCCTT CCTCAAAGAT 
TTCTACAACA TTCATCCGAA TACACTGGCC AAATCAGAGC AGCAGGTGGC CGAGATCCGG 
CGCGAACTGG AAATCACCGT GTCTGGCAAT GAACTACCAC ACCCGGTGGT CAGTTTCGAG 
GAGAGCTCGC TGCCCGCTCA CGTCATTGAA GAGATGAAGC GCCAGGGATT CACCAAGCCC 
ACTGCTATCC AATCGCAGGG TTGGCCCATT GCCTTAAGTG GCCGCGATTT GGTGGGCATT 
GCCCAGACCG GTTCAGGCAA GACGCTGGCA TACATGCTGC CTGCCATCGT GCACATTGGC 
AATCAGCCGC CAATAATTCG CGGCGAAGGA CCCATTGCTC TAGTGTTGGC CCCCACCCGG 
GAGCTGGCCC AGCAGATCCA ATCTGTCGTG CGAGACTATG GACATCTGTG CAAGCCAGAG 
ATTCGTCACA CTTGCATCTT TGGCGGCTCA TCGAAGGTGC CGCAGGCACG CGATCTTGAC 
CGCGGCGTCG AGGTGATCAT CGCCACACCA GGACGTCTGA TTGATTTCCT TGAGAATCGC 
AACACTAACT TGCAGAGATG CACTTATCTG GTGCTGGACG AAGCCGACCG CATGCTGGAC 
ATGGGTTTCG AGCCGCAGAT CCGTAAGATC ATTGAACAGA TTCGTCCCGA CCGTCAGGTG 
GTCATGTGGT CCGCAACCTG GCCAAAGGAG GTGCAGGCCC TAGCCGGAGA CTTTTTAAAC 
GATTATATCC AAATCAACAT TGGTTCGATG AACCTATCGG CTAACCACAA CATTCGCCAG 
ATTGTCGAGA TCTGTACAGA AATAGAGAAG CCGCAGCGCT TAGTATGCTT GCTCAATGAG 
ATTTCTCCCA TCAAGAATTC GGGAAACAAT GGGAACAAAA TTATCGTCTT TGTGGAGACC 
AAGATCAAGG TGGAGGACAT CTTGCAGATC ATTCGAGCCG AGGGTTACAA CGCCACCTCA 
ATTCACGGCG ACAAGACCCA GAATGAGCGT GATTCAGTGC TAAAGGATTT CCGTAACGGC 
AAGTCCAACA TTCTGATTGC CACCGATGTG GCTTCGCGCG GCCTCGATGT CGAGGATCTG 
CAGTACGTAA TTAACTATGA TTACCCGAAC TCCTCGGAGA ACTACGTGCA CCGTATCGGG 
CGCACTGGTC GCTGCCAGCA GCTGGGAACT GCCTACACAT TTTTCACGCC TGACAATGCC 
AAGCAGGCCC GTGAACTGAT CTCTGTGCTC GAGGAGGCTG GCCAGACGCC GTCGCAGGCT 
CTTTTGGATC TTGCTCGTTC GATGCCCAGC TCGGGCGGCT ATCGCGGCAA CAAGCGCTGG 
AACAACAACA GCGGCGGGGA TCGCAACACT GGCGGTAACA ACGGCATCTA TCAGCAGCGC 
AACAACAATC CGCTGAATTA CCAGGCCGGT AACAACTATA ACAACAATCG CAGCGGCCCC 
CCAACTGGCA GTAGCTACCA GCAGTATGCC GCTGGCGGCA ATACCTATCT GCAGAATGGT 
GCTGGTGGTG GCGTTAATTG GAATCGCATG AACCAGATGG GACAGGATGG AGGAGCCGGA 
CAACAGCAGC GAAACGGCAA CACCTATCGC CCGAGGGCTC CGTTCAACAA TGGCGGACCA 
CGCGCCATTA TGGGCCATCA GGGTGGCGGC GTCGGAGCTG GTGGAGCAGC CGGCGTAGGC 
GGTGGCCCAC AGCAGCTCAT GGCACCCCAG CAGGGCGGAC AGTATATGCC ACAAGCATAC 
CGCGGACGTA GCCAGTTTGT GCCGCAGGGA GCTGGTGCCG GTGGCATGCC GCCACGATTC 
CAGCAGTATC CCAAGCGGGA GTACCAGCAA CAAGGAGTAA CCCACTACGG TCAGCAGGCT 
TTGCAGCATC AGCAACAACA GCAGCAGGCT GGCCAACAGA CCAAGCCGCC CAAGGACGAA 
AGCAACAAGT ATGTTCCGTC GGCTGCGGTG ACCAGCTCGC TGGTCCAATA CACGCCAGCC 
AATTCGCCAA CGATTGCGGC CGTAGCTGCT GCCGCTGCAG CAGCCGCCGG ACGAGCTGCA 
GCTGCAGCTG TGGCTCCGGC TCCCGGTGCC GCCTATATGT ACGATGCGGC CGGTGTTCTC 
ACTACCGCCG CTGCGCCGGG CACCAATCCA TATGCCAACC AGTTCAGCAT GCCGGCCACC 
TACTATGCCG CCCAACATCA CCAGTTCGCC CAAGCCGTGG CAGGACCCGG ACCAGCACCC 
GGCCAGGCCA TCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mahe cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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