gt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gt
Entrez Gene ID	31227
Full Name	giant
Synonyms	CG7952,DM17,Dmel\CG7952,EG:BACH7M4.5,GIAN_DROME,GT,Giant,Gt,l(1)3Aa,l(1)giant
General protein information	Preferred Names giant Names CG7952 gene product from transcript CG7952-RB CG7952-PB gt-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A3-3A3\|1-0.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080310.3	NP_525049.1	giant

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

there are two phases of transcriptional regulation for the gap gene giant in Drosophila
Title: A quantitative validated model reveals two phases of transcriptional regulation for the gap gene giant in Drosophila.

two polycomb response elements-containing regions at the Polycomb-group proteins target gene, giant, one at the promoter, and another approximately 6 kb upstream.
Title: Mapping polycomb response elements at the Drosophilla melanogaster giant locus.

gt likely controls ecdysone production indirectly by contributing the peptidergic phenotype of the PTTH-producing neurons in the embryo
Title: The Drosophila gap gene giant regulates ecdysone production through specification of the PTTH-producing neurons.

Results showed Gt repression roles in the regulation of two groups of partially overlapping stripes and that Gt morphogen activity is part of the mechanism responsible for the differential positioning of these stripes borders.
Title: Investigating giant (Gt) repression in the formation of partially overlapping pair-rule stripes.

The Drosophila gap gene giant has an anterior segment identity function mediated through disconnected and teashirt.
Title: The Drosophila gap gene giant has an anterior segment identity function mediated through disconnected and teashirt.

The following gt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00603	NM_080310.3 Latest version!	Drosophila melanogaster giant (gt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gt ( NM_080310.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525049.1 Drosophila melanogaster giant (gt), mRNA.

Price & Availability↑

CloneID	OFa00603
Clone ID Related Accession (Same CDS sequence)	NM_080310.3
Accession Version	NM_080310.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	giant
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080310.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAATGC ACGAGAAACT CATGGCCGGG CAGTTCTTCG ATCTCAAGAC TGATCGCAAG 
CCCCTGATGC ACCACCACCA GTACCAGCAC CACCAGCAGC AACCACTGCA CCACTTGCCG 
CACAGCCAAT TGCCGGTTCA GGGATCCTTG GGCCTACCCA AAATGGATCT GTACACGGCC 
TACGCCTACC AGCAGCAGTT GCTGGGAGCT GCCCTCAGTC AGCAGCAACA ACAGCAACAG 
CAGCAGCAGC AACATCAGCA GCTGCAGCAG CAGCATACCT CCTCTGCAGA GGTCCTGGAT 
CTTTCCCGTC GATGTGACAG CGTAGAGACA CCCAGGAAGA CTCCCTCGCC GTATCAAACA 
AGCTATAGCT ACGGCAGTGG TTCCCCCTCG GCTTCGCCCA CCAGCAATCT TCTGTATGCC 
GCCCAAATGC AACAGCAGCA ACATCAGCAG CAACAACAGC AACAGCAGCA GCAGCAACAA 
TTAGCCTCCC TGTATCCCGC TTTTTACTAC AGCAACATCA AGCAGGAGCA AGCCACGCCC 
ACTGCTGCTC CGCCCAAGGT TACACCAACC GCTAACCTTC TGCAAACCTT TGCTGCCGCC 
TCTGCTGCGG CCGCCGCTGC TGCTGCCGCT TCTTCGACCA ACTCACCAAG ACCCGCCAGC 
AACGCCAGCA CCATGCAGAT AGACGTTCTG GAGAATCCCC TGTCACCGGC TGTTGAGGCC 
ACCACGCCAA CCACCTCCAG CAGCGGCGAG GCGGGAAAGA ACACCCGTCC CTTCAAGGCC 
TTTCCCCGGG ATCCCTTGGT CATAGCTGCC AATTTCGCAG CCACTGATGT CCTTTTGGAC 
AATCCGCGAG TGGAGCGCTA CACGGAATAC CGGAAGCGAG TGCTTGAGCA AATCCGCAGC 
TCCAACGGAG GATCGCGCAC CGTAACCAAC CCAAAAATGC GAAGGACCAA CTCAAGAAGT 
GGATCCGTCA ACGAAGGCAG CTCCTCAAAC AACAACAGCG AAAGCGAGGA TCGCGCTGCT 
GCGGAGGAGT CAAGCGACTG TGACTCCCAG GCGGGCAACT TCGAGAGCAA GTCCGCCACC 
AGCAGCTCCA GCAACTTGGC TAACGCTACT GCAGCAAACT CGGGCATCAG CTCGGGCAGC 
CAGGTTAAGG ATGCCGCATA CTACGAGAGG CGTCGCAAGA ACAATGCTGC CGCCAAGAAG 
TCCCGCGATC GCCGCCGCAT CAAGGAGGAT GAGATCGCCA TCAGGGCCGC CTATCTGGAA 
CGCCAGAACA TCGAGCTCTT GTGCCAGATC GACGCCCTCA AGGTCCAGCT GGCCGCCTTC 
ACCTCCGCCA AAGTAACCAC CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525049.1
CDS	116..1462
Translation	MLMHEKLMAGQFFDLKTDRKPLMHHHQYQHHQQQPLHHLPHSQLPVQGSLGLPKMDLYTAYAYQQQLLGAALSQQQQQQQQQQQHQQLQQQHTSSAEVLDLSRRCDSVETPRKTPSPYQTSYSYGSGSPSASPTSNLLYAAQMQQQQHQQQQQQQQQQQQLASLYPAFYYSNIKQEQATPTAAPPKVTPTANLLQTFAAASAAAAAAAAASSTNSPRPASNASTMQIDVLENPLSPAVEATTPTTSSSGEAGKNTRPFKAFPRDPLVIAANFAATDVLLDNPRVERYTEYRKRVLEQIRSSNGGSRTVTNPKMRRTNSRSGSVNEGSSSNNNSESEDRAAAEESSDCDSQAGNFESKSATSSSSNLANATAANSGISSGSQVKDAAYYERRRKNNAAAKKSRDRRRIKEDEIAIRAAYLERQNIELLCQIDALKVQLAAFTSAKVTTA

Target ORF information:

RefSeq Version	NM_080310.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster giant (gt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080310.3

ORF Insert Sequence:

ATGCTAATGC ACGAGAAACT CATGGCCGGG CAGTTCTTCG ATCTCAAGAC TGATCGCAAG 
CCCCTGATGC ACCACCACCA GTACCAGCAC CACCAGCAGC AACCACTGCA CCACTTGCCG 
CACAGCCAAT TGCCGGTTCA GGGATCCTTG GGCCTACCCA AAATGGATCT GTACACGGCC 
TACGCCTACC AGCAGCAGTT GCTGGGAGCT GCCCTCAGTC AGCAGCAACA ACAGCAACAG 
CAGCAGCAGC AACATCAGCA GCTGCAGCAG CAGCATACCT CCTCTGCAGA GGTCCTGGAT 
CTTTCCCGTC GATGTGACAG CGTAGAGACA CCCAGGAAGA CTCCCTCGCC GTATCAAACA 
AGCTATAGCT ACGGCAGTGG TTCCCCCTCG GCTTCGCCCA CCAGCAATCT TCTGTATGCC 
GCCCAAATGC AACAGCAGCA ACATCAGCAG CAACAACAGC AACAGCAGCA GCAGCAACAA 
TTAGCCTCCC TGTATCCCGC TTTTTACTAC AGCAACATCA AGCAGGAGCA AGCCACGCCC 
ACTGCTGCTC CGCCCAAGGT TACACCAACC GCTAACCTTC TGCAAACCTT TGCTGCCGCC 
TCTGCTGCGG CCGCCGCTGC TGCTGCCGCT TCTTCGACCA ACTCACCAAG ACCCGCCAGC 
AACGCCAGCA CCATGCAGAT AGACGTTCTG GAGAATCCCC TGTCACCGGC TGTTGAGGCC 
ACCACGCCAA CCACCTCCAG CAGCGGCGAG GCGGGAAAGA ACACCCGTCC CTTCAAGGCC 
TTTCCCCGGG ATCCCTTGGT CATAGCTGCC AATTTCGCAG CCACTGATGT CCTTTTGGAC 
AATCCGCGAG TGGAGCGCTA CACGGAATAC CGGAAGCGAG TGCTTGAGCA AATCCGCAGC 
TCCAACGGAG GATCGCGCAC CGTAACCAAC CCAAAAATGC GAAGGACCAA CTCAAGAAGT 
GGATCCGTCA ACGAAGGCAG CTCCTCAAAC AACAACAGCG AAAGCGAGGA TCGCGCTGCT 
GCGGAGGAGT CAAGCGACTG TGACTCCCAG GCGGGCAACT TCGAGAGCAA GTCCGCCACC 
AGCAGCTCCA GCAACTTGGC TAACGCTACT GCAGCAAACT CGGGCATCAG CTCGGGCAGC 
CAGGTTAAGG ATGCCGCATA CTACGAGAGG CGTCGCAAGA ACAATGCTGC CGCCAAGAAG 
TCCCGCGATC GCCGCCGCAT CAAGGAGGAT GAGATCGCCA TCAGGGCCGC CTATCTGGAA 
CGCCAGAACA TCGAGCTCTT GTGCCAGATC GACGCCCTCA AGGTCCAGCT GGCCGCCTTC 
ACCTCCGCCA AAGTAACCAC CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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