ind cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ind
Entrez Gene ID	2768974
Full Name	intermediate neuroblasts defective
Synonyms	CG11551,Dmel\CG11551,Ind,ind/Gsx
General protein information	Preferred Names intermediate neuroblasts defective Names CG11551 gene product from transcript CG11551-RB CG11551-PB ind-PB intermediate nerve cord defective
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 71B2-71B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_206365.3	NP_996087.2	intermediate neuroblasts defective

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ERK signaling during Drosophila embryogenesis, is at a stage when ERK induces transcription of intermediate neuroblasts defective (ind), a gene essential for patterning of the nerve cord.
Title: Kinetics of gene derepression by ERK signaling.

EGF signaling, acting through MAP kinase, impinges on multiple aspects of Ind regulatory activity.
Title: Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression.

cross-repression between Ind and Msh stabilizes the border between intermediate and dorsal tritocerebrum and deutocerebrum, and that both transcription factors are competent to inhibit vnd expression
Title: Role of en and novel interactions between msh, ind, and vnd in dorsoventral patterning of the Drosophila brain and ventral nerve cord.

expression is expanded ventrally in ind mutant embryos and Ind protein directly and specifically bound to the msh regulatory DNA, and this interaction was required to limit the ventral boundary of msh expression.
Title: Ind represses msh expression in the intermediate column of the Drosophila neuroectoderm, through direct interaction with upstream regulatory DNA.

The function of Ind in regulating vnd expression is one of refinement and maintenance of the dorsal border.
Title: Identification of Ind transcription activation and repression domains required for dorsoventral patterning of the CNS.

The following ind gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ind cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20052	NM_206365.3 Latest version!	Drosophila melanogaster intermediate neuroblasts defective (ind), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ind ( NM_206365.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996087.2 Drosophila melanogaster intermediate neuroblasts defective (ind), mRNA.

Price & Availability↑

CloneID	OFa20052
Clone ID Related Accession (Same CDS sequence)	NM_206365.3
Accession Version	NM_206365.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	intermediate neuroblasts defective
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_206365.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGTT CATTTTTGAT GGATTCCCTA CTCAGCGATA GGCCCAATTT GAGCCAGAAG 
AAGGAGAAAC TGGGATCGCC AGGAGGAAGT CCTACAGCCG CTGCTGCAGT GGCCGCAGCT 
GCCATGTTAC CATCGATACC CATGCTTCCT TACCCGGCCA GCTATGTGGG CAGCTATCTC 
TTCTCCTTGG GCATCCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAACA GCACGCAGCA 
GCTGCAGCAG CGGCCGCAGC AGCAGCAGCT GCCTTGCAGC AACATCCACA TGTCAGTTCG 
AGTCCCGGTT CCCTTTACCA TCCATACGCC CAGCTGTTCG CCAGCAAGAG GAAGTCCAGT 
GGATTCAGCA ACTACGAAGG ATGTTATCCA TCGCCACCGC TCTCGGCCAA TCCCAACTCC 
CAGCAATTGC CGCCAATACA CAACCTCTAC GGATCTCCAG TTGTGGGCGG ATTACCCTTA 
CCGGAACCTG GAAGCTTCTG CACATCACCG TCCGCTTCAT CGTCCGCCTC TTTGGATTAT 
ACCAATAACT TTGATGAACC TCAAGGAAAG CGTTTCAAGC ACGAGTCCTC CTGCTCTCCG 
AATAGCTCAC CACTGAAGAA CCACTCTTCG GGAGGTCCCG TCGAGATCAC GCCGCTGATC 
AACGATTATG CCGATTCCAG CAAAAGGATC CGCACTGCCT TCACCAGCAC CCAGTTGCTG 
GAGCTCGAGC GAGAATTCTC GCACAACGCC TACCTATCCC GCCTACGTCG CATCGAAATC 
GCCAATAGAT TGCGCCTTTC CGAGAAGCAG GTGAAGATCT GGTTCCAGAA TCGCCGAGTC 
AAGCAGAAAA AGGGCGGCTC CGAAAGTCCC ACCTTCAATC TGTCCACCAA CTCCAATGGA 
AGTCCGCAAG CATCTCCAGT GTCTCCCCAA GTCAAAGTCC ACGAATTGAA GGTTGAGGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996087.2
CDS	61..1023
Translation	MSRSFLMDSLLSDRPNLSQKKEKLGSPGGSPTAAAAVAAAAMLPSIPMLPYPASYVGSYLFSLGIQQQQQQQQQQQQHAAAAAAAAAAAAALQQHPHVSSSPGSLYHPYAQLFASKRKSSGFSNYEGCYPSPPLSANPNSQQLPPIHNLYGSPVVGGLPLPEPGSFCTSPSASSSASLDYTNNFDEPQGKRFKHESSCSPNSSPLKNHSSGGPVEITPLINDYADSSKRIRTAFTSTQLLELEREFSHNAYLSRLRRIEIANRLRLSEKQVKIWFQNRRVKQKKGGSESPTFNLSTNSNGSPQASPVSPQVKVHELKVEA

Target ORF information:

RefSeq Version	NM_206365.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster intermediate neuroblasts defective (ind), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206365.3

ORF Insert Sequence:

ATGTCGCGTT CATTTTTGAT GGATTCCCTA CTCAGCGATA GGCCCAATTT GAGCCAGAAG 
AAGGAGAAAC TGGGATCGCC AGGAGGAAGT CCTACAGCCG CTGCTGCAGT GGCCGCAGCT 
GCCATGTTAC CATCGATACC CATGCTTCCT TACCCGGCCA GCTATGTGGG CAGCTATCTC 
TTCTCCTTGG GCATCCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAACA GCACGCAGCA 
GCTGCAGCAG CGGCCGCAGC AGCAGCAGCT GCCTTGCAGC AACATCCACA TGTCAGTTCG 
AGTCCCGGTT CCCTTTACCA TCCATACGCC CAGCTGTTCG CCAGCAAGAG GAAGTCCAGT 
GGATTCAGCA ACTACGAAGG ATGTTATCCA TCGCCACCGC TCTCGGCCAA TCCCAACTCC 
CAGCAATTGC CGCCAATACA CAACCTCTAC GGATCTCCAG TTGTGGGCGG ATTACCCTTA 
CCGGAACCTG GAAGCTTCTG CACATCACCG TCCGCTTCAT CGTCCGCCTC TTTGGATTAT 
ACCAATAACT TTGATGAACC TCAAGGAAAG CGTTTCAAGC ACGAGTCCTC CTGCTCTCCG 
AATAGCTCAC CACTGAAGAA CCACTCTTCG GGAGGTCCCG TCGAGATCAC GCCGCTGATC 
AACGATTATG CCGATTCCAG CAAAAGGATC CGCACTGCCT TCACCAGCAC CCAGTTGCTG 
GAGCTCGAGC GAGAATTCTC GCACAACGCC TACCTATCCC GCCTACGTCG CATCGAAATC 
GCCAATAGAT TGCGCCTTTC CGAGAAGCAG GTGAAGATCT GGTTCCAGAA TCGCCGAGTC 
AAGCAGAAAA AGGGCGGCTC CGAAAGTCCC ACCTTCAATC TGTCCACCAA CTCCAATGGA 
AGTCCGCAAG CATCTCCAGT GTCTCCCCAA GTCAAAGTCC ACGAATTGAA GGTTGAGGCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ind cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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