mute cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mute
Entrez Gene ID	2768848
Full Name	muscle wasted
Synonyms	CG15610,CG33455,CG33456,CG34415,Dmel\CG34415,Dmel_CG33455,Dmel_CG33456,Mute
General protein information	Preferred Names muscle wasted Names CG34415 gene product from transcript CG34415-RC CG34415-PC CG34415-PD mute-PC mute-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 53E2-53E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_206141.2	NP_995863.1	muscle wasted, isoform D
NM_001103882.3	NP_001097352.1	muscle wasted, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mute gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mute cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11792	NM_206141.2 Latest version!	Drosophila melanogaster muscle wasted (mute), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11791	NM_001103882.3 Latest version!	Drosophila melanogaster muscle wasted (mute), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mute ( NM_206141.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995863.1 Drosophila melanogaster muscle wasted (mute), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11792
Clone ID Related Accession (Same CDS sequence)	NM_206141.2
Accession Version	NM_206141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscle wasted, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206141.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCTGCA GCAATACAAA CTCTAAAACG GCAGCGCAGC CAAAGAGATC TAGGAGTGAT 
GATGAGGCGG CGAAGCTGAG AAGAGCCAAC CGGCAGGAGG AGATGCTTCG CCAAATGCTC 
CAGCCGGATA CTACAGAAGA GCGCAATCGC AAAGATGCCA TTTTTGCCAT GAATTTTTAT 
GAAAAGGTAG AGGAGGCCTT GCTGAGTGCC AATCGCCTAG ATGATTGCAA AAAATTCAAC 
AGTATTTTGC AAAACTTCGA CCCCAGGCAG GAGAAAGTTT CATCTTTGTA TTTGAAAGTG 
GAAGAAATTT TGCTACCCAC TCATCCCGAA CTGGCTGAAG TATTCCTCAA CTTTTTGCTA 
CCTGCCGAGG CCGCTGAGAT GGGAAAATTC TTTGAGCACT TTATGATATG CAATGCTACC 
AACTTTATCA ACAAACTTAA CATTTACTTC AGTAAGCAGC CAGCTCAAAT CAGGAAGATT 
TATGCCTGTC TAAACGAGTT GGCCGAAATG CCTAATGTCA GCATGAAAAA AGTTGAAAAT 
AAGATAATGC CCTTGCTCAA GGGCAATCAA TTTCTGATCG ATTGGTTTGT GCAACAATTC 
CCACAGGGAA AACCACCAAA AAGAATCCTT TCTCCTGTAG AGACCATAAA TTTGCATGAC 
GTTCAAAACA ATACAACGGG AGAATATACG GAAACTATTC AAGAGTTTAC AGATGTACCT 
ACGCCAAGTA AACCAGGTTG TCACTTAAAG TATATCAATG GTCGCATATT TTATGGAGCC 
AAGATCCTAT TGCCTGCCAA ACTGTCTTTT ATGGCTGCCA GTATTTACAA TGAACAATCT 
CATGACGTGC CACCCGTCTC CAGTGACTCG ATAAACTGCG CCCATGGTAT TCGTGCCCAG 
GGAGAAAAAC TTCTTAGTAT GGCTTCCAAT ACAGATAGCG ACGATGGCGC GGATCGGAGT 
GAGGAAGATG ATGCCAGCCG CGCCTTGATT ATTGATACCA CGAGTGATGA GCAGAACAGC 
TGCTCATCTA TTGAGATGTG CGATGAGACA GGCTTAAGAG CACATGCAAT CAGATTGAAT 
CCCGGCTTGT ATACCAACTC AAGCTTTAAC AATGCAGCGA CCAGTACGCC CAATCTGGGC 
AACTCTCACC AATCCCATCA TCCGCACGCC AAAAAAACTG CCATAAACTT TGGTGAAATA 
TCCCCACGCA AGCAATCGGC ATTTAACAAC TCGGGATTGA GTCCCTTATC GGCGGCCCCC 
AACAGTTCCC CTCAAGTCGA CAAACGAAAG TCACCTACTA AGAAGATCCG ATCTCCTCTT 
AATCAAACTC AGAGCAGACA ACGTGCCAAT AATCAGCCAA TGGTTGTTAT CCATGAGTCT 
CAGCCTTCAT CTTCGCCAGC CATTGAGTGT GCAAAGCGCT TGCGCACGCT GATCGATCAG 
GAGAGCGTGC GGGATGCAGC AGATACAAAG GCGGGCATTC GAATTATTGC GCAAGCCAAG 
CAGCAACCTC CAAACCAATG CAGCTCTGAA GCTATTACGA AAGTCGAGGT AGTCAGCCTG 
GATGAAAGTG CCGAGAATAT GCCTTTCTGT ACAAATCTCA AAATGGACAC AGATCAATCG 
GAGGAGGAGC TGGAGCCACT GCAATTAAGT GCTGGCAACA CACCACAGCA CGTGATCACA 
ACACAATTCG ATAGCGATGA GGACTGCAAG CTGGATGTTG TGGCCACGCT GGCGAACTGC 
ACCACCACCG AAGATAGATC GGAATCGGCA GCGGCATTAC CTGCCACCAC ATCGCACTCT 
GAGTCTGGTA GTTCCCACTC GGCACCTTGG ACTCGTGAAG AGGACAAAGT AATCCTGATC 
GAAATGAAAA TAGGGGCACG GGATCGAGAG CAGCTCATTC GGCGTATGCG CGCCAAGCTA 
AAGAATCGCA GTATAGAAGA ACTACGTAGT AGGCATCAGT TTCTTATGGA CTTTCTGTCC 
AAATTGCAGG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995863.1
CDS	923..2920
Translation	MFCSNTNSKTAAQPKRSRSDDEAAKLRRANRQEEMLRQMLQPDTTEERNRKDAIFAMNFYEKVEEALLSANRLDDCKKFNSILQNFDPRQEKVSSLYLKVEEILLPTHPELAEVFLNFLLPAEAAEMGKFFEHFMICNATNFINKLNIYFSKQPAQIRKIYACLNELAEMPNVSMKKVENKIMPLLKGNQFLIDWFVQQFPQGKPPKRILSPVETINLHDVQNNTTGEYTETIQEFTDVPTPSKPGCHLKYINGRIFYGAKILLPAKLSFMAASIYNEQSHDVPPVSSDSINCAHGIRAQGEKLLSMASNTDSDDGADRSEEDDASRALIIDTTSDEQNSCSSIEMCDETGLRAHAIRLNPGLYTNSSFNNAATSTPNLGNSHQSHHPHAKKTAINFGEISPRKQSAFNNSGLSPLSAAPNSSPQVDKRKSPTKKIRSPLNQTQSRQRANNQPMVVIHESQPSSSPAIECAKRLRTLIDQESVRDAADTKAGIRIIAQAKQQPPNQCSSEAITKVEVVSLDESAENMPFCTNLKMDTDQSEEELEPLQLSAGNTPQHVITTQFDSDEDCKLDVVATLANCTTTEDRSESAAALPATTSHSESGSSHSAPWTREEDKVILIEMKIGARDREQLIRRMRAKLKNRSIEELRSRHQFLMDFLSKLQGK

Target ORF information:

RefSeq Version	NM_206141.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscle wasted (mute), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206141.2

ORF Insert Sequence:

ATGTTCTGCA GCAATACAAA CTCTAAAACG GCAGCGCAGC CAAAGAGATC TAGGAGTGAT 
GATGAGGCGG CGAAGCTGAG AAGAGCCAAC CGGCAGGAGG AGATGCTTCG CCAAATGCTC 
CAGCCGGATA CTACAGAAGA GCGCAATCGC AAAGATGCCA TTTTTGCCAT GAATTTTTAT 
GAAAAGGTAG AGGAGGCCTT GCTGAGTGCC AATCGCCTAG ATGATTGCAA AAAATTCAAC 
AGTATTTTGC AAAACTTCGA CCCCAGGCAG GAGAAAGTTT CATCTTTGTA TTTGAAAGTG 
GAAGAAATTT TGCTACCCAC TCATCCCGAA CTGGCTGAAG TATTCCTCAA CTTTTTGCTA 
CCTGCCGAGG CCGCTGAGAT GGGAAAATTC TTTGAGCACT TTATGATATG CAATGCTACC 
AACTTTATCA ACAAACTTAA CATTTACTTC AGTAAGCAGC CAGCTCAAAT CAGGAAGATT 
TATGCCTGTC TAAACGAGTT GGCCGAAATG CCTAATGTCA GCATGAAAAA AGTTGAAAAT 
AAGATAATGC CCTTGCTCAA GGGCAATCAA TTTCTGATCG ATTGGTTTGT GCAACAATTC 
CCACAGGGAA AACCACCAAA AAGAATCCTT TCTCCTGTAG AGACCATAAA TTTGCATGAC 
GTTCAAAACA ATACAACGGG AGAATATACG GAAACTATTC AAGAGTTTAC AGATGTACCT 
ACGCCAAGTA AACCAGGTTG TCACTTAAAG TATATCAATG GTCGCATATT TTATGGAGCC 
AAGATCCTAT TGCCTGCCAA ACTGTCTTTT ATGGCTGCCA GTATTTACAA TGAACAATCT 
CATGACGTGC CACCCGTCTC CAGTGACTCG ATAAACTGCG CCCATGGTAT TCGTGCCCAG 
GGAGAAAAAC TTCTTAGTAT GGCTTCCAAT ACAGATAGCG ACGATGGCGC GGATCGGAGT 
GAGGAAGATG ATGCCAGCCG CGCCTTGATT ATTGATACCA CGAGTGATGA GCAGAACAGC 
TGCTCATCTA TTGAGATGTG CGATGAGACA GGCTTAAGAG CACATGCAAT CAGATTGAAT 
CCCGGCTTGT ATACCAACTC AAGCTTTAAC AATGCAGCGA CCAGTACGCC CAATCTGGGC 
AACTCTCACC AATCCCATCA TCCGCACGCC AAAAAAACTG CCATAAACTT TGGTGAAATA 
TCCCCACGCA AGCAATCGGC ATTTAACAAC TCGGGATTGA GTCCCTTATC GGCGGCCCCC 
AACAGTTCCC CTCAAGTCGA CAAACGAAAG TCACCTACTA AGAAGATCCG ATCTCCTCTT 
AATCAAACTC AGAGCAGACA ACGTGCCAAT AATCAGCCAA TGGTTGTTAT CCATGAGTCT 
CAGCCTTCAT CTTCGCCAGC CATTGAGTGT GCAAAGCGCT TGCGCACGCT GATCGATCAG 
GAGAGCGTGC GGGATGCAGC AGATACAAAG GCGGGCATTC GAATTATTGC GCAAGCCAAG 
CAGCAACCTC CAAACCAATG CAGCTCTGAA GCTATTACGA AAGTCGAGGT AGTCAGCCTG 
GATGAAAGTG CCGAGAATAT GCCTTTCTGT ACAAATCTCA AAATGGACAC AGATCAATCG 
GAGGAGGAGC TGGAGCCACT GCAATTAAGT GCTGGCAACA CACCACAGCA CGTGATCACA 
ACACAATTCG ATAGCGATGA GGACTGCAAG CTGGATGTTG TGGCCACGCT GGCGAACTGC 
ACCACCACCG AAGATAGATC GGAATCGGCA GCGGCATTAC CTGCCACCAC ATCGCACTCT 
GAGTCTGGTA GTTCCCACTC GGCACCTTGG ACTCGTGAAG AGGACAAAGT AATCCTGATC 
GAAATGAAAA TAGGGGCACG GGATCGAGAG CAGCTCATTC GGCGTATGCG CGCCAAGCTA 
AAGAATCGCA GTATAGAAGA ACTACGTAGT AGGCATCAGT TTCTTATGGA CTTTCTGTCC 
AAATTGCAGG GAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mute ( NM_001103882.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097352.1 Drosophila melanogaster muscle wasted (mute), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11791
Clone ID Related Accession (Same CDS sequence)	NM_001103882.3
Accession Version	NM_001103882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscle wasted, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103882.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAGCA GTGCAAAGAA GTCGGACTCT GCAAAGAGCA ATAGCTCGAC GCCACGAAGA 
CCGCGAAAGC GCAAACGAGT GGATCGCCAG AGCCTGGACT CGCAGGTCGA TGAGGATGCC 
AAGGAGATCG ATCGCCGGCT ACGGAAAGTG GCCAAGAAGA ACTCCATCAG TGCCGAGGAC 
ATGCAAAAGG TGGTCCGGAA GGTGGTGCGC AACGATCATG TTCTGGCATT AGTCACTTTG 
AAGGCGGAGG ACGAGCTGGC GCGGGAGAAG TTGGAGTCGG CGGAGCAGCA AAAGCGGGGT 
GCCATCATCA TAACGGACAC TCCATCTGTG CCCAAGCTAA CGCGAGCGAA AGCACGGGAA 
CTGAACTGCA CGCCTGGGAT CTCGCTGCCC CAGCTCAATG AAACTTCCAC CGACAATAAT 
GGTATAGAGG CTCTAATACG TGAGGATTTG CACTCTGATG AGGAGGACGA GGAGTATACC 
TTCAAGGAGG AGGACTTTCA TTCCGATGAT GATCCTAACA CTACAGCATC GGATTTCGAC 
TCCAATCCCT GCACGCCTCA AACTCCTCTT ACTGCCAATG AGGAATCTCC TATCAAATTC 
TCTGATGACG GTTGTTTCAA GGTGCCGTTG GACAGAAATT TTCAGGATAA AGAGGATCTT 
AGGATTGCCA CTAGAACGCG CTCCAAATTT TGTTTGCAGC AAACTACCAT TGAGGATTTG 
CAGTCGGAAT TTGTCCCGCC GGACGTGGAG CCTAGCGATG TGGTGGAGAA CGAAATGACC 
GCCAACGATG CGGATTGGAT GCAGTTCCTC AATGACTTTA CAAAGCCTTT AAACAATACG 
GTCCAGGGCG ACGATGATGA TCCTATTAAC GATCCAGAAT ATGTGGCCGC TGATCACTTG 
GAATCGGAAT ATGAGAACGC CGAGGAGCTG CGCGATGTAA ATATATCTAA AAAAGAGTTA 
ACTGACCTGG TCTCTGAGCT GTTTGCTGGT TTGCTGGAAG AAGGAGTCTC TCTGGAATCG 
ATTGAATTGG AAACACCTCA AAAATTCGTT GAAAGTGCCA ACGTTTCGAT GATTCCCATT 
GATCAAGTGT CCCGTAACAT CGATTTTGAT GGCCAGCAGA TAAATGCTAA TCAGCAGGAG 
CTGTCCGTTT TTCAACCTAA TCTAATGCCG CCTCCTTGCG AAGGGAACGA AATGGGCATG 
GGTGTGCCTG TCCCTGTGGC TGTGCTGCAG GAGGAAACTG GTGGATCCGC TTGCAATGTG 
GTAAACATAC CTTCCGAGGC GCTGACTGTT CCCCCCGAAC TAATAGCTGT TCCAATACCT 
GGGCAACCTA ATTGCTTCCA AATCGCCAAA GTTGTAGGCA ATGATTCAAC TGTTGGTCTT 
CCATTAACTG AAGAGACACT GCAGAACTTT TCTAGCTTGC ATCCAATGGA ACCCCCCGTG 
GTAGCTGCAA GCAATGGAGA TCGATATAAA AAACCATTTG ATACGAACTT CACCTGGGAA 
TACATCTCGG TTAGGAAACA TATATATTCG GAGTATGAGG AAAAGTTTGA GAGCCTGCGA 
AACGTAGAGC CAGTAAACCT CAATCATCTC GCCAGGAGCA GGGGATTTAC CCAGTCTCAG 
CATGATATGC TTCAGCAGCA GCTTCGCATT CACACGCAGT TGCTCAGTCA AAGCTATCTG 
CAAACCTACT CCCATCCTAC GTTGTACCCC ATGGCCAAGA AGCCCAGAGA AATGTTGGAA 
GAATTGCACA AAATGGCAAC TAAAGATCCC AGTTTTAATT GCTGGAATCT GCAGGAGGCT 
GTTCAGCTGG TTAGAAAATG GGAACATGAT CTGTCCAGTG ATGAGTTTAA GGAGGAGAAC 
GAGAAGATGA TGGACTTCAT CAATAAGGAA ACGGATCTGA CTGATGGCCA CACGCGCCAG 
GTTCCCAGGC TGGCGCCTCG TATTATGGAT CTCATGCTGG ATAGCCGGGT GTTTATGTAT 
CCGCAGTACC TACCCCGTAT GGCGTTTCAA CCGAAACTGA CTCAAACCAC CGCTTACGCA 
CCATCCGAAT ATCAGCTTAT TGCCATTGGC CTAGAGAAGC ACTTGGCATT AATGGTAAAA 
GCCAAGAGAC AACTACGCAA AGGTGCAGAT CCCATTCGCG TTGCCTGTAA GCGAATGATT 
AAGGATACGA TTTGTGGAAA GACTGCCCGC CGAATTTTCT TGAAAATCGC GGAGCTACGC 
AACTCAAAAC AGTACAATCC AGTGAAGTAT TTCTTTGAAC ATCGTCGAGC ACCGCCCGCT 
AACGCCATCC TTTGGGGATT CGCTGGTGGG CTGGTGAGAA CTCCGCGGGA ACGACACATG 
GAGCTTCCCA GTGGCTGGCA ATACTACATT GAGAAAACTT GGGATCAAAA GCGCACACGT 
CGCAATTTTA AAGCATCGAA AGCATCGTCG GTGGCTAGTG CTTCTTATTT GGAGTTTGTA 
CGCGAGGCGG TCGGCGAGGA CCTGCTGCTG CCAGAGACTT TTGGTCAGGC TGCGGTCCCC 
AATGGCAGTG CTGCTGGTTC GGAACCGCCC AAGGAGAGCA AACCGAAGAA AAGGCCAGCG 
CAAAGGAGAC CCCGCCCTGC GGAAAACCCA CCACAGCTTA CCATCAATGT GAATTATGTT 
TTTGGCGGGA ATACGAGCGC GGGAAACATA CCCGGGGTCT CTGGGGTTTC GGTGCCCTTG 
GTGGCCGGCG GAAATCTGGC CGAGAACGTA CTGAACATCA ATCGCAGCTT ATACACCGCC 
GCCGAGTGCA GTAGTCTGCT GGCCTTGCCG CCTGCTCCTC CACCATTGGA TGAAAATGCT 
CCGCCAGCCA TCAACTTCAC AGTAGACAAG TTCACTAACA CACTACAAAT CTGCGAATTG 
GAGGAATCCA GCAAGGCCGT ACAGCCGGAT CTGTTGCCTG CGCAGGATAC CCCTTCCGTC 
CCAGGCAGAA ACGCCAAAAG GGCGCGATAC CAAATCTTTA AGCCAAAGGT TCAATCCGCC 
AATAAATCGC GAAAAGGGTC AACACCAACC CGCAGTCGAT ACCATATATT GCGCGAACGT 
TTGCGCCATA AACTAAGCCA GCGCTGTCAT TCCCTAATCA CCACCTATGA ATCCTACCTG 
CGTCATCACA TCGAAAAATA TAAAGACTGC GAGTTGGTAC AGGTCGTGCA CAATCGTTTT 
TTCGCCCTCG AACTGTACAC TCAACTTCTG GTCGATCTGA GGATGTTCTG CAGCAATACA 
AACTCTAAAA CGGCAGCGCA GCCAAAGAGA TCTAGGAGTG ATGATGAGGC GGCGAAGCTG 
AGAAGAGCCA ACCGGCAGGA GGAGATGCTT CGCCAAATGC TCCAGCCGGA TACTACAGAA 
GAGCGCAATC GCAAAGATGC CATTTTTGCC ATGAATTTTT ATGAAAAGGT AGAGGAGGCC 
TTGCTGAGTG CCAATCGCCT AGATGATTGC AAAAAATTCA ACAGTATTTT GCAAAACTTC 
GACCCCAGGC AGGAGAAAGT TTCATCTTTG TATTTGAAAG TGGAAGAAAT TTTGCTACCC 
ACTCATCCCG AACTGGCTGA AGTATTCCTC AACTTTTTGC TACCTGCCGA GGCCGCTGAG 
ATGGGAAAAT TCTTTGAGCA CTTTATGATA TGCAATGCTA CCAACTTTAT CAACAAACTT 
AACATTTACT TCAGTAAGCA GCCAGCTCAA ATCAGGAAGA TTTATGCCTG TCTAAACGAG 
TTGGCCGAAA TGCCTAATGT CAGCATGAAA AAAGTTGAAA ATAAGATAAT GCCCTTGCTC 
AAGGGCAATC AATTTCTGAT CGATTGGTTT GTGCAACAAT TCCCACAGGG AAAACCACCA 
AAAAGAATCC TTTCTCCTGT AGAGACCATA AATTTGCATG ACGTTCAAAA CAATACAACG 
GGAGAATATA CGGAAACTAT TCAAGAGTTT ACAGATGTAC CTACGCCAAG TAAACCAGGT 
TGTCACTTAA AGTATATCAA TGGTCGCATA TTTTATGGAG CCAAGATCCT ATTGCCTGCC 
AAACTGTCTT TTATGGCTGC CAGTATTTAC AATGAACAAT CTCATGACGT GCCACCCGTC 
TCCAGTGACT CGATAAACTG CGCCCATGGT ATTCGTGCCC AGGGAGAAAA ACTTCTTAGT 
ATGGCTTCCA ATACAGATAG CGACGATGGC GCGGATCGGA GTGAGGAAGA TGATGCCAGC 
CGCGCCTTGA TTATTGATAC CACGAGTGAT GAGCAGAACA GCTGCTCATC TATTGAGATG 
TGCGATGAGA CAGGCTTAAG AGCACATGCA ATCAGATTGA ATCCCGGCTT GTATACCAAC 
TCAAGCTTTA ACAATGCAGC GACCAGTACG CCCAATCTGG GCAACTCTCA CCAATCCCAT 
CATCCGCACG CCAAAAAAAC TGCCATAAAC TTTGGTGAAA TATCCCCACG CAAGCAATCG 
GCATTTAACA ACTCGGGATT GAGTCCCTTA TCGGCGGCCC CCAACAGTTC CCCTCAAGTC 
GACAAACGAA AGTCACCTAC TAAGAAGATC CGATCTCCTC TTAATCAAAC TCAGAGCAGA 
CAACGTGCCA ATAATCAGCC AATGGTTGTT ATCCATGAGT CTCAGCCTTC ATCTTCGCCA 
GCCATTGAGT GTGCAAAGCG CTTGCGCACG CTGATCGATC AGGAGAGCGT GCGGGATGCA 
GCAGATACAA AGGCGGGCAT TCGAATTATT GCGCAAGCCA AGCAGCAACC TCCAAACCAA 
TGCAGCTCTG AAGCTATTAC GAAAGTCGAG GTAGTCAGCC TGGATGAAAG TGCCGAGAAT 
ATGCCTTTCT GTACAAATCT CAAAATGGAC ACAGATCAAT CGGAGGAGGA GCTGGAGCCA 
CTGCAATTAA GTGCTGGCAA CACACCACAG CACGTGATCA CAACACAATT CGATAGCGAT 
GAGGACTGCA AGCTGGATGT TGTGGCCACG CTGGCGAACT GCACCACCAC CGAAGATAGA 
TCGGAATCGG CAGCGGCATT ACCTGCCACC ACATCGCACT CTGAGTCTGG TAGTTCCCAC 
TCGGCACCTT GGACTCGTGA AGAGGACAAA GTAATCCTGA TCGAAATGAA AATAGGGGCA 
CGGGATCGAG AGCAGCTCAT TCGGCGTATG CGCGCCAAGC TAAAGAATCG CAGTATAGAA 
GAACTACGTA GTAGGCATCA GTTTCTTATG GACTTTCTGT CCAAATTGCA GGGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097352.1
CDS	112..5331
Translation	MPSSAKKSDSAKSNSSTPRRPRKRKRVDRQSLDSQVDEDAKEIDRRLRKVAKKNSISAEDMQKVVRKVVRNDHVLALVTLKAEDELAREKLESAEQQKRGAIIITDTPSVPKLTRAKARELNCTPGISLPQLNETSTDNNGIEALIREDLHSDEEDEEYTFKEEDFHSDDDPNTTASDFDSNPCTPQTPLTANEESPIKFSDDGCFKVPLDRNFQDKEDLRIATRTRSKFCLQQTTIEDLQSEFVPPDVEPSDVVENEMTANDADWMQFLNDFTKPLNNTVQGDDDDPINDPEYVAADHLESEYENAEELRDVNISKKELTDLVSELFAGLLEEGVSLESIELETPQKFVESANVSMIPIDQVSRNIDFDGQQINANQQELSVFQPNLMPPPCEGNEMGMGVPVPVAVLQEETGGSACNVVNIPSEALTVPPELIAVPIPGQPNCFQIAKVVGNDSTVGLPLTEETLQNFSSLHPMEPPVVAASNGDRYKKPFDTNFTWEYISVRKHIYSEYEEKFESLRNVEPVNLNHLARSRGFTQSQHDMLQQQLRIHTQLLSQSYLQTYSHPTLYPMAKKPREMLEELHKMATKDPSFNCWNLQEAVQLVRKWEHDLSSDEFKEENEKMMDFINKETDLTDGHTRQVPRLAPRIMDLMLDSRVFMYPQYLPRMAFQPKLTQTTAYAPSEYQLIAIGLEKHLALMVKAKRQLRKGADPIRVACKRMIKDTICGKTARRIFLKIAELRNSKQYNPVKYFFEHRRAPPANAILWGFAGGLVRTPRERHMELPSGWQYYIEKTWDQKRTRRNFKASKASSVASASYLEFVREAVGEDLLLPETFGQAAVPNGSAAGSEPPKESKPKKRPAQRRPRPAENPPQLTINVNYVFGGNTSAGNIPGVSGVSVPLVAGGNLAENVLNINRSLYTAAECSSLLALPPAPPPLDENAPPAINFTVDKFTNTLQICELEESSKAVQPDLLPAQDTPSVPGRNAKRARYQIFKPKVQSANKSRKGSTPTRSRYHILRERLRHKLSQRCHSLITTYESYLRHHIEKYKDCELVQVVHNRFFALELYTQLLVDLRMFCSNTNSKTAAQPKRSRSDDEAAKLRRANRQEEMLRQMLQPDTTEERNRKDAIFAMNFYEKVEEALLSANRLDDCKKFNSILQNFDPRQEKVSSLYLKVEEILLPTHPELAEVFLNFLLPAEAAEMGKFFEHFMICNATNFINKLNIYFSKQPAQIRKIYACLNELAEMPNVSMKKVENKIMPLLKGNQFLIDWFVQQFPQGKPPKRILSPVETINLHDVQNNTTGEYTETIQEFTDVPTPSKPGCHLKYINGRIFYGAKILLPAKLSFMAASIYNEQSHDVPPVSSDSINCAHGIRAQGEKLLSMASNTDSDDGADRSEEDDASRALIIDTTSDEQNSCSSIEMCDETGLRAHAIRLNPGLYTNSSFNNAATSTPNLGNSHQSHHPHAKKTAINFGEISPRKQSAFNNSGLSPLSAAPNSSPQVDKRKSPTKKIRSPLNQTQSRQRANNQPMVVIHESQPSSSPAIECAKRLRTLIDQESVRDAADTKAGIRIIAQAKQQPPNQCSSEAITKVEVVSLDESAENMPFCTNLKMDTDQSEEELEPLQLSAGNTPQHVITTQFDSDEDCKLDVVATLANCTTTEDRSESAAALPATTSHSESGSSHSAPWTREEDKVILIEMKIGARDREQLIRRMRAKLKNRSIEELRSRHQFLMDFLSKLQGK

Target ORF information:

RefSeq Version	NM_001103882.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscle wasted (mute), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103882.3

ORF Insert Sequence:

ATGCCGAGCA GTGCAAAGAA GTCGGACTCT GCAAAGAGCA ATAGCTCGAC GCCACGAAGA 
CCGCGAAAGC GCAAACGAGT GGATCGCCAG AGCCTGGACT CGCAGGTCGA TGAGGATGCC 
AAGGAGATCG ATCGCCGGCT ACGGAAAGTG GCCAAGAAGA ACTCCATCAG TGCCGAGGAC 
ATGCAAAAGG TGGTCCGGAA GGTGGTGCGC AACGATCATG TTCTGGCATT AGTCACTTTG 
AAGGCGGAGG ACGAGCTGGC GCGGGAGAAG TTGGAGTCGG CGGAGCAGCA AAAGCGGGGT 
GCCATCATCA TAACGGACAC TCCATCTGTG CCCAAGCTAA CGCGAGCGAA AGCACGGGAA 
CTGAACTGCA CGCCTGGGAT CTCGCTGCCC CAGCTCAATG AAACTTCCAC CGACAATAAT 
GGTATAGAGG CTCTAATACG TGAGGATTTG CACTCTGATG AGGAGGACGA GGAGTATACC 
TTCAAGGAGG AGGACTTTCA TTCCGATGAT GATCCTAACA CTACAGCATC GGATTTCGAC 
TCCAATCCCT GCACGCCTCA AACTCCTCTT ACTGCCAATG AGGAATCTCC TATCAAATTC 
TCTGATGACG GTTGTTTCAA GGTGCCGTTG GACAGAAATT TTCAGGATAA AGAGGATCTT 
AGGATTGCCA CTAGAACGCG CTCCAAATTT TGTTTGCAGC AAACTACCAT TGAGGATTTG 
CAGTCGGAAT TTGTCCCGCC GGACGTGGAG CCTAGCGATG TGGTGGAGAA CGAAATGACC 
GCCAACGATG CGGATTGGAT GCAGTTCCTC AATGACTTTA CAAAGCCTTT AAACAATACG 
GTCCAGGGCG ACGATGATGA TCCTATTAAC GATCCAGAAT ATGTGGCCGC TGATCACTTG 
GAATCGGAAT ATGAGAACGC CGAGGAGCTG CGCGATGTAA ATATATCTAA AAAAGAGTTA 
ACTGACCTGG TCTCTGAGCT GTTTGCTGGT TTGCTGGAAG AAGGAGTCTC TCTGGAATCG 
ATTGAATTGG AAACACCTCA AAAATTCGTT GAAAGTGCCA ACGTTTCGAT GATTCCCATT 
GATCAAGTGT CCCGTAACAT CGATTTTGAT GGCCAGCAGA TAAATGCTAA TCAGCAGGAG 
CTGTCCGTTT TTCAACCTAA TCTAATGCCG CCTCCTTGCG AAGGGAACGA AATGGGCATG 
GGTGTGCCTG TCCCTGTGGC TGTGCTGCAG GAGGAAACTG GTGGATCCGC TTGCAATGTG 
GTAAACATAC CTTCCGAGGC GCTGACTGTT CCCCCCGAAC TAATAGCTGT TCCAATACCT 
GGGCAACCTA ATTGCTTCCA AATCGCCAAA GTTGTAGGCA ATGATTCAAC TGTTGGTCTT 
CCATTAACTG AAGAGACACT GCAGAACTTT TCTAGCTTGC ATCCAATGGA ACCCCCCGTG 
GTAGCTGCAA GCAATGGAGA TCGATATAAA AAACCATTTG ATACGAACTT CACCTGGGAA 
TACATCTCGG TTAGGAAACA TATATATTCG GAGTATGAGG AAAAGTTTGA GAGCCTGCGA 
AACGTAGAGC CAGTAAACCT CAATCATCTC GCCAGGAGCA GGGGATTTAC CCAGTCTCAG 
CATGATATGC TTCAGCAGCA GCTTCGCATT CACACGCAGT TGCTCAGTCA AAGCTATCTG 
CAAACCTACT CCCATCCTAC GTTGTACCCC ATGGCCAAGA AGCCCAGAGA AATGTTGGAA 
GAATTGCACA AAATGGCAAC TAAAGATCCC AGTTTTAATT GCTGGAATCT GCAGGAGGCT 
GTTCAGCTGG TTAGAAAATG GGAACATGAT CTGTCCAGTG ATGAGTTTAA GGAGGAGAAC 
GAGAAGATGA TGGACTTCAT CAATAAGGAA ACGGATCTGA CTGATGGCCA CACGCGCCAG 
GTTCCCAGGC TGGCGCCTCG TATTATGGAT CTCATGCTGG ATAGCCGGGT GTTTATGTAT 
CCGCAGTACC TACCCCGTAT GGCGTTTCAA CCGAAACTGA CTCAAACCAC CGCTTACGCA 
CCATCCGAAT ATCAGCTTAT TGCCATTGGC CTAGAGAAGC ACTTGGCATT AATGGTAAAA 
GCCAAGAGAC AACTACGCAA AGGTGCAGAT CCCATTCGCG TTGCCTGTAA GCGAATGATT 
AAGGATACGA TTTGTGGAAA GACTGCCCGC CGAATTTTCT TGAAAATCGC GGAGCTACGC 
AACTCAAAAC AGTACAATCC AGTGAAGTAT TTCTTTGAAC ATCGTCGAGC ACCGCCCGCT 
AACGCCATCC TTTGGGGATT CGCTGGTGGG CTGGTGAGAA CTCCGCGGGA ACGACACATG 
GAGCTTCCCA GTGGCTGGCA ATACTACATT GAGAAAACTT GGGATCAAAA GCGCACACGT 
CGCAATTTTA AAGCATCGAA AGCATCGTCG GTGGCTAGTG CTTCTTATTT GGAGTTTGTA 
CGCGAGGCGG TCGGCGAGGA CCTGCTGCTG CCAGAGACTT TTGGTCAGGC TGCGGTCCCC 
AATGGCAGTG CTGCTGGTTC GGAACCGCCC AAGGAGAGCA AACCGAAGAA AAGGCCAGCG 
CAAAGGAGAC CCCGCCCTGC GGAAAACCCA CCACAGCTTA CCATCAATGT GAATTATGTT 
TTTGGCGGGA ATACGAGCGC GGGAAACATA CCCGGGGTCT CTGGGGTTTC GGTGCCCTTG 
GTGGCCGGCG GAAATCTGGC CGAGAACGTA CTGAACATCA ATCGCAGCTT ATACACCGCC 
GCCGAGTGCA GTAGTCTGCT GGCCTTGCCG CCTGCTCCTC CACCATTGGA TGAAAATGCT 
CCGCCAGCCA TCAACTTCAC AGTAGACAAG TTCACTAACA CACTACAAAT CTGCGAATTG 
GAGGAATCCA GCAAGGCCGT ACAGCCGGAT CTGTTGCCTG CGCAGGATAC CCCTTCCGTC 
CCAGGCAGAA ACGCCAAAAG GGCGCGATAC CAAATCTTTA AGCCAAAGGT TCAATCCGCC 
AATAAATCGC GAAAAGGGTC AACACCAACC CGCAGTCGAT ACCATATATT GCGCGAACGT 
TTGCGCCATA AACTAAGCCA GCGCTGTCAT TCCCTAATCA CCACCTATGA ATCCTACCTG 
CGTCATCACA TCGAAAAATA TAAAGACTGC GAGTTGGTAC AGGTCGTGCA CAATCGTTTT 
TTCGCCCTCG AACTGTACAC TCAACTTCTG GTCGATCTGA GGATGTTCTG CAGCAATACA 
AACTCTAAAA CGGCAGCGCA GCCAAAGAGA TCTAGGAGTG ATGATGAGGC GGCGAAGCTG 
AGAAGAGCCA ACCGGCAGGA GGAGATGCTT CGCCAAATGC TCCAGCCGGA TACTACAGAA 
GAGCGCAATC GCAAAGATGC CATTTTTGCC ATGAATTTTT ATGAAAAGGT AGAGGAGGCC 
TTGCTGAGTG CCAATCGCCT AGATGATTGC AAAAAATTCA ACAGTATTTT GCAAAACTTC 
GACCCCAGGC AGGAGAAAGT TTCATCTTTG TATTTGAAAG TGGAAGAAAT TTTGCTACCC 
ACTCATCCCG AACTGGCTGA AGTATTCCTC AACTTTTTGC TACCTGCCGA GGCCGCTGAG 
ATGGGAAAAT TCTTTGAGCA CTTTATGATA TGCAATGCTA CCAACTTTAT CAACAAACTT 
AACATTTACT TCAGTAAGCA GCCAGCTCAA ATCAGGAAGA TTTATGCCTG TCTAAACGAG 
TTGGCCGAAA TGCCTAATGT CAGCATGAAA AAAGTTGAAA ATAAGATAAT GCCCTTGCTC 
AAGGGCAATC AATTTCTGAT CGATTGGTTT GTGCAACAAT TCCCACAGGG AAAACCACCA 
AAAAGAATCC TTTCTCCTGT AGAGACCATA AATTTGCATG ACGTTCAAAA CAATACAACG 
GGAGAATATA CGGAAACTAT TCAAGAGTTT ACAGATGTAC CTACGCCAAG TAAACCAGGT 
TGTCACTTAA AGTATATCAA TGGTCGCATA TTTTATGGAG CCAAGATCCT ATTGCCTGCC 
AAACTGTCTT TTATGGCTGC CAGTATTTAC AATGAACAAT CTCATGACGT GCCACCCGTC 
TCCAGTGACT CGATAAACTG CGCCCATGGT ATTCGTGCCC AGGGAGAAAA ACTTCTTAGT 
ATGGCTTCCA ATACAGATAG CGACGATGGC GCGGATCGGA GTGAGGAAGA TGATGCCAGC 
CGCGCCTTGA TTATTGATAC CACGAGTGAT GAGCAGAACA GCTGCTCATC TATTGAGATG 
TGCGATGAGA CAGGCTTAAG AGCACATGCA ATCAGATTGA ATCCCGGCTT GTATACCAAC 
TCAAGCTTTA ACAATGCAGC GACCAGTACG CCCAATCTGG GCAACTCTCA CCAATCCCAT 
CATCCGCACG CCAAAAAAAC TGCCATAAAC TTTGGTGAAA TATCCCCACG CAAGCAATCG 
GCATTTAACA ACTCGGGATT GAGTCCCTTA TCGGCGGCCC CCAACAGTTC CCCTCAAGTC 
GACAAACGAA AGTCACCTAC TAAGAAGATC CGATCTCCTC TTAATCAAAC TCAGAGCAGA 
CAACGTGCCA ATAATCAGCC AATGGTTGTT ATCCATGAGT CTCAGCCTTC ATCTTCGCCA 
GCCATTGAGT GTGCAAAGCG CTTGCGCACG CTGATCGATC AGGAGAGCGT GCGGGATGCA 
GCAGATACAA AGGCGGGCAT TCGAATTATT GCGCAAGCCA AGCAGCAACC TCCAAACCAA 
TGCAGCTCTG AAGCTATTAC GAAAGTCGAG GTAGTCAGCC TGGATGAAAG TGCCGAGAAT 
ATGCCTTTCT GTACAAATCT CAAAATGGAC ACAGATCAAT CGGAGGAGGA GCTGGAGCCA 
CTGCAATTAA GTGCTGGCAA CACACCACAG CACGTGATCA CAACACAATT CGATAGCGAT 
GAGGACTGCA AGCTGGATGT TGTGGCCACG CTGGCGAACT GCACCACCAC CGAAGATAGA 
TCGGAATCGG CAGCGGCATT ACCTGCCACC ACATCGCACT CTGAGTCTGG TAGTTCCCAC 
TCGGCACCTT GGACTCGTGA AGAGGACAAA GTAATCCTGA TCGAAATGAA AATAGGGGCA 
CGGGATCGAG AGCAGCTCAT TCGGCGTATG CGCGCCAAGC TAAAGAATCG CAGTATAGAA 
GAACTACGTA GTAGGCATCA GTTTCTTATG GACTTTCTGT CCAAATTGCA GGGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mute cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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