MtnE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MtnE
Entrez Gene ID	10178932
Full Name	Metallothionein E
Synonyms	CG42872,DMG8-chr3R.29.007,Dmel\CG42872
General protein information	Preferred Names Metallothionein E Names CG42872 gene product from transcript CG42872-RB CG42872-PA CG42872-PB MtnE-PA MtnE-PB metallothionein E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F1-92F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202325.2	NP_001189254.1	metallothionein E, isoform A
NM_001300498.1	NP_001287427.1	metallothionein E, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that metallothionein E (MtnE) is the least metal-specific isoform of the Drosophila MTs, suggesting it could play a role when/where a broad spectrum of metal coordination abilities are advantageous in terms of physiological needs.
Title: Is MtnE, the fifth Drosophila metallothionein, functionally distinct from the other members of this polymorphic protein family?

a new fifth member of Drosophila MT gene family, coding for metallothionein E
Title: Characterization of MtnE, the fifth metallothionein member in Drosophila.

The following MtnE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MtnE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07462	NM_001202325.2 Latest version!	Drosophila melanogaster metallothionein E, transcript variant A (MtnE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa07462	NM_001300498.1 Latest version!	Drosophila melanogaster metallothionein E, transcript variant B (MtnE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MtnE ( NM_001202325.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189254.1 Drosophila melanogaster metallothionein E, transcript variant A (MtnE), mRNA.

Price & Availability↑

CloneID	OFa07462
Clone ID Related Accession (Same CDS sequence)	NM_001202325.2 , NM_001300498.1
Accession Version	NM_001202325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	metallothionein E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001202325.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

1
61
121

ATGCCTTGCA AGGGATGTGG AAACAACTGC CAGTGCTCAG CCGGAAAGTG CGGAGGTAAC 
TGCGCCGGAA ACAGCCAATG CCAATGCGCC GCCAAGACGG GAGCCAAGTG CTGCCAGGCC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189254.1
CDS	71..196
Translation	MPCKGCGNNCQCSAGKCGGNCAGNSQCQCAAKTGAKCCQAK

Target ORF information:

RefSeq Version	NM_001202325.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster metallothionein E, transcript variant A (MtnE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202325.2

ORF Insert Sequence:

1
61
121

ATGCCTTGCA AGGGATGTGG AAACAACTGC CAGTGCTCAG CCGGAAAGTG CGGAGGTAAC 
TGCGCCGGAA ACAGCCAATG CCAATGCGCC GCCAAGACGG GAGCCAAGTG CTGCCAGGCC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MtnE ( NM_001300498.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287427.1 Drosophila melanogaster metallothionein E, transcript variant B (MtnE), mRNA.

Price & Availability↑

CloneID	OFa07462
Clone ID Related Accession (Same CDS sequence)	NM_001202325.2 , NM_001300498.1
Accession Version	NM_001300498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	metallothionein E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

1
61
121

ATGCCTTGCA AGGGATGTGG AAACAACTGC CAGTGCTCAG CCGGAAAGTG CGGAGGTAAC 
TGCGCCGGAA ACAGCCAATG CCAATGCGCC GCCAAGACGG GAGCCAAGTG CTGCCAGGCC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287427.1
CDS	71..196
Translation	MPCKGCGNNCQCSAGKCGGNCAGNSQCQCAAKTGAKCCQAK

Target ORF information:

RefSeq Version	NM_001300498.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster metallothionein E, transcript variant B (MtnE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300498.1

ORF Insert Sequence:

1
61
121

ATGCCTTGCA AGGGATGTGG AAACAACTGC CAGTGCTCAG CCGGAAAGTG CGGAGGTAAC 
TGCGCCGGAA ACAGCCAATG CCAATGCGCC GCCAAGACGG GAGCCAAGTG CTGCCAGGCC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MtnE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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