LOC109546548 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546548
Entrez Gene ID	109546548
Full Name	espin
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019917540.1	XP_019773099.1	espin isoform X2
XM_019917539.1	XP_019773098.1	espin isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109546548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35526	XM_019917539.1 Latest version!	Dendroctonus ponderosae espin (LOC109546548), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODe35527	XM_019917540.1 Latest version!	Dendroctonus ponderosae espin (LOC109546548), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546548 ( XM_019917539.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773098.1 Dendroctonus ponderosae espin (LOC109546548), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe35526
Clone ID Related Accession (Same CDS sequence)	XM_019917539.1
Accession Version	XM_019917539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	espin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851160.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTC TGTCGAGGAA ACGAAGATTT AGGAGCAACT CTTGTAGATT GAGCGCTAAT 
ACTCAGATGG ACAATGACGT GACACCAGTT TATTTAGCTG CCCAAGAAGG CCATCTCGAT 
GTCCTCAAGT TTCTCGTTTT AGAAGCAGGA GGTTCTCTAT ACGTGAGAGC CAAAGATGGA 
ATGGCTCCTA TTCACGCTGC TAGCCAGATG GGATGTTTAA ATTGCGTTAA ATGGATGATT 
CAAGATCAAG GTGTCGACCC TAATCTGAGA GATGGAGATG GAGCGACTCC ATTGCATTTT 
GCCGCCTCCA GGGGTCACTT GGATACTGTG AGATGGTTGC TTAAACACGG CGCAAGATTG 
AGCTTGGATA AGTTTGGAAA AAGCCCCATA AATGATGCTG CTGAAAATCA GCAAGTAGAA 
TGTCTGAACA TCTTGGTGCA ACACGGTACC ACTCCAGATT ATAACGATTC TGATAGACCC 
AACAACGGAA AAAGTTGTTC TTGTGCAAGA AAAGGAGATC ACATAAAACG AGGAAATAGC 
ACAGGCTCTG ATTGCTCAAC CTGTAAGCCC AAACAGGCTG TAGGTAACTC AAAAAACTCC 
AAAAGCTCTT TGGCATCTCT GGAGCCTTTC TATTTACACC CTCCCCTCAA TGGTAGGAGC 
ATGGAACCAG CACACGCTCC AGAGAATGGG CTTTATATCA ATCCGATGAC CAATCATAGA 
CACAGTGCGT CAACTTGCTC AGATACCAGC TCAATAGGAA GTGAGTCTTT CTACCTTCAT 
AATCCCAGTG AAGTGAGCAA AGAAGTGGTG TACAATCGAG TCAAGGGGTT GTTCGATTCG 
AATGGGGAGC AACGACCAAT GTCGGGATTA AAAGTGAAAG TTGAAGTTCA TTCCTCATCA 
TCCGGTGCAG GATCTGACGA AAATCTGTCA TCTTCGGATT TATCTTCAGT TCGATCGAAT 
GACCACGAAA ACGACTATGA GGACATTTAC CTCGTTAGAG AAGAAGCACG AAATCAAGAA 
AGAACTAACA TTCATAATAT AAATGGACGA TCACGATCCA GAGACTCTGG TTCACATAGC 
AGATCAGGTT CGATCAGCTC ATCCAATTCA GGATGTAACG TAATAGTGAA AATGACTCCC 
AATGGAAACA CCAAGAACGG AATATACAAA TCTAACGAGT TCCTACCTAA ACCTCAGAAA 
ATTGAAAAGT TAACCGACAG AAAACCTATG CAAGATAGAG AGCGAACTTC AAACATTTCA 
TCAGTTCCGG TAGAGGCTTC GCCACCTCCA CCACCACCTC CACCTCCGAA CAACCCCAGC 
TCACTCATTG ACCCCAATGA CCCCCATTTG AATGAACCCA GGGGAGCTGA AATCGTCGAA 
GTGGTTGAAG AACCCACCCT TAGACCTTCG GACATTGTGA AGGGCATGTG CAGAAGTATG 
TCAGCTCTAT CTGCTCGTCG GTTGAATTCT TCCTGTTCCG ACCTCACAAT CGTCGGTCTT 
AACGACGAAG ACAGACAGTC AACATCCAGC ACTATTCTAG CTACACCAGT GAATCGTCAA 
CGAGTTTTAC CATTTGTGCC TCCCAGCTTT CCACTAGCTG GCTCGAACAA GCTAATCAAA 
CCGTCTGAAT TTCTTAGAAG TATCAGCTCA GCTGACAAAC GAAATGATGG TCCCAGAGAA 
ATATCGATGA GTGAGGATGA TAAAAAAGCT GATATGGACA AGGAAGAAGA ACAGACCAAT 
AGCGACAGTA AAGTTGTTGG GCCTCCACCG CCACCCTTGC CAGAGTTTAA TGGCGATTTA 
ACACAAAACG CCATTTCAAA ACAAAGCACC TTAACACACC AGGAGAAAGT AAAAAAGCAT 
CAACCTTTGT CAGCGATTAG CATCCAAGAC CTGAACTCGG TCCAGCTGAG AAGAACTGAA 
AAACTTGTTG CTTCTAAGAC TTTCTCAGCA CCCACTAGAA GTGTATCATT GCAATGCCTC 
AATACGGCTG ATTCATTCAT GGCTCAGAAA ACTGACCTAA TCGCCGAGCT CAAAATGTCG 
AAGGATTTAA CTGGACTGAA GAAAATGAAG ATCGAAAAAG CGAAAACGGA ACAGACATTG 
GAAAGAGAAG TAATAGTTGA AATTCGAAAA CAGTTTACTG CTAACAACTT TATAGAGAAG 
GCAAGTATAT ACAAGAGCGG ATTGAGTCGC CACGATGCAC CATTATCAAT TCCAGACAAA 
GACAATACTG GAAACCTAAT TCCTGCTTGG AAAAGGCAAA TGCTAGCTAA GAAAGCTGCA 
GAGAAGGCCA AGAAGGAAAT GGAAGAACAG CTGCAAAAAG ATGCTGAAGA AAAGCGTTTA 
CAATCCATCC CTCAATGGAA ACGACCATTG CTGGCCAAGA AGGAAGAGAC GGAAAATAAA 
ATAAGGCAAA TGATCTACAC GCCGAAAGTA GTGGAAGAGC CAAAGGTAAA ACTCCCTGAC 
ATGAGACAAC AACAACCGAA TAACGAAAAA GAACAGGAAG ATATTAACAA CAATCACATG 
AAGGAATACG ATAAAGAAAG CAATATGACT TTGAGTAACA ACTCTTTGGA CGACGAACCC 
TCTGATGAGG AACCCAATAT TATTCCTTGG AGGGCACAAT TACGAAAAAC AAACAGTTCT 
TTGAATCTTC TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773098.1
CDS	278..2935
Translation	MTTLSRKRRFRSNSCRLSANTQMDNDVTPVYLAAQEGHLDVLKFLVLEAGGSLYVRAKDGMAPIHAASQMGCLNCVKWMIQDQGVDPNLRDGDGATPLHFAASRGHLDTVRWLLKHGARLSLDKFGKSPINDAAENQQVECLNILVQHGTTPDYNDSDRPNNGKSCSCARKGDHIKRGNSTGSDCSTCKPKQAVGNSKNSKSSLASLEPFYLHPPLNGRSMEPAHAPENGLYINPMTNHRHSASTCSDTSSIGSESFYLHNPSEVSKEVVYNRVKGLFDSNGEQRPMSGLKVKVEVHSSSSGAGSDENLSSSDLSSVRSNDHENDYEDIYLVREEARNQERTNIHNINGRSRSRDSGSHSRSGSISSSNSGCNVIVKMTPNGNTKNGIYKSNEFLPKPQKIEKLTDRKPMQDRERTSNISSVPVEASPPPPPPPPPNNPSSLIDPNDPHLNEPRGAEIVEVVEEPTLRPSDIVKGMCRSMSALSARRLNSSCSDLTIVGLNDEDRQSTSSTILATPVNRQRVLPFVPPSFPLAGSNKLIKPSEFLRSISSADKRNDGPREISMSEDDKKADMDKEEEQTNSDSKVVGPPPPPLPEFNGDLTQNAISKQSTLTHQEKVKKHQPLSAISIQDLNSVQLRRTEKLVASKTFSAPTRSVSLQCLNTADSFMAQKTDLIAELKMSKDLTGLKKMKIEKAKTEQTLEREVIVEIRKQFTANNFIEKASIYKSGLSRHDAPLSIPDKDNTGNLIPAWKRQMLAKKAAEKAKKEMEEQLQKDAEEKRLQSIPQWKRPLLAKKEETENKIRQMIYTPKVVEEPKVKLPDMRQQQPNNEKEQEDINNNHMKEYDKESNMTLSNNSLDDEPSDEEPNIIPWRAQLRKTNSSLNLLC

Target ORF information:

RefSeq Version	XM_019917539.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae espin (LOC109546548), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917539.1

ORF Insert Sequence:

ATGACGACTC TGTCGAGGAA ACGAAGATTT AGGAGCAACT CTTGTAGATT GAGCGCTAAT 
ACTCAGATGG ACAATGACGT GACACCAGTT TATTTAGCTG CCCAAGAAGG CCATCTCGAT 
GTCCTCAAGT TTCTCGTTTT AGAAGCAGGA GGTTCTCTAT ACGTGAGAGC CAAAGATGGA 
ATGGCTCCTA TTCACGCTGC TAGCCAGATG GGATGTTTAA ATTGCGTTAA ATGGATGATT 
CAAGATCAAG GTGTCGACCC TAATCTGAGA GATGGAGATG GAGCGACTCC ATTGCATTTT 
GCCGCCTCCA GGGGTCACTT GGATACTGTG AGATGGTTGC TTAAACACGG CGCAAGATTG 
AGCTTGGATA AGTTTGGAAA AAGCCCCATA AATGATGCTG CTGAAAATCA GCAAGTAGAA 
TGTCTGAACA TCTTGGTGCA ACACGGTACC ACTCCAGATT ATAACGATTC TGATAGACCC 
AACAACGGAA AAAGTTGTTC TTGTGCAAGA AAAGGAGATC ACATAAAACG AGGAAATAGC 
ACAGGCTCTG ATTGCTCAAC CTGTAAGCCC AAACAGGCTG TAGGTAACTC AAAAAACTCC 
AAAAGCTCTT TGGCATCTCT GGAGCCTTTC TATTTACACC CTCCCCTCAA TGGTAGGAGC 
ATGGAACCAG CACACGCTCC AGAGAATGGG CTTTATATCA ATCCGATGAC CAATCATAGA 
CACAGTGCGT CAACTTGCTC AGATACCAGC TCAATAGGAA GTGAGTCTTT CTACCTTCAT 
AATCCCAGTG AAGTGAGCAA AGAAGTGGTG TACAATCGAG TCAAGGGGTT GTTCGATTCG 
AATGGGGAGC AACGACCAAT GTCGGGATTA AAAGTGAAAG TTGAAGTTCA TTCCTCATCA 
TCCGGTGCAG GATCTGACGA AAATCTGTCA TCTTCGGATT TATCTTCAGT TCGATCGAAT 
GACCACGAAA ACGACTATGA GGACATTTAC CTCGTTAGAG AAGAAGCACG AAATCAAGAA 
AGAACTAACA TTCATAATAT AAATGGACGA TCACGATCCA GAGACTCTGG TTCACATAGC 
AGATCAGGTT CGATCAGCTC ATCCAATTCA GGATGTAACG TAATAGTGAA AATGACTCCC 
AATGGAAACA CCAAGAACGG AATATACAAA TCTAACGAGT TCCTACCTAA ACCTCAGAAA 
ATTGAAAAGT TAACCGACAG AAAACCTATG CAAGATAGAG AGCGAACTTC AAACATTTCA 
TCAGTTCCGG TAGAGGCTTC GCCACCTCCA CCACCACCTC CACCTCCGAA CAACCCCAGC 
TCACTCATTG ACCCCAATGA CCCCCATTTG AATGAACCCA GGGGAGCTGA AATCGTCGAA 
GTGGTTGAAG AACCCACCCT TAGACCTTCG GACATTGTGA AGGGCATGTG CAGAAGTATG 
TCAGCTCTAT CTGCTCGTCG GTTGAATTCT TCCTGTTCCG ACCTCACAAT CGTCGGTCTT 
AACGACGAAG ACAGACAGTC AACATCCAGC ACTATTCTAG CTACACCAGT GAATCGTCAA 
CGAGTTTTAC CATTTGTGCC TCCCAGCTTT CCACTAGCTG GCTCGAACAA GCTAATCAAA 
CCGTCTGAAT TTCTTAGAAG TATCAGCTCA GCTGACAAAC GAAATGATGG TCCCAGAGAA 
ATATCGATGA GTGAGGATGA TAAAAAAGCT GATATGGACA AGGAAGAAGA ACAGACCAAT 
AGCGACAGTA AAGTTGTTGG GCCTCCACCG CCACCCTTGC CAGAGTTTAA TGGCGATTTA 
ACACAAAACG CCATTTCAAA ACAAAGCACC TTAACACACC AGGAGAAAGT AAAAAAGCAT 
CAACCTTTGT CAGCGATTAG CATCCAAGAC CTGAACTCGG TCCAGCTGAG AAGAACTGAA 
AAACTTGTTG CTTCTAAGAC TTTCTCAGCA CCCACTAGAA GTGTATCATT GCAATGCCTC 
AATACGGCTG ATTCATTCAT GGCTCAGAAA ACTGACCTAA TCGCCGAGCT CAAAATGTCG 
AAGGATTTAA CTGGACTGAA GAAAATGAAG ATCGAAAAAG CGAAAACGGA ACAGACATTG 
GAAAGAGAAG TAATAGTTGA AATTCGAAAA CAGTTTACTG CTAACAACTT TATAGAGAAG 
GCAAGTATAT ACAAGAGCGG ATTGAGTCGC CACGATGCAC CATTATCAAT TCCAGACAAA 
GACAATACTG GAAACCTAAT TCCTGCTTGG AAAAGGCAAA TGCTAGCTAA GAAAGCTGCA 
GAGAAGGCCA AGAAGGAAAT GGAAGAACAG CTGCAAAAAG ATGCTGAAGA AAAGCGTTTA 
CAATCCATCC CTCAATGGAA ACGACCATTG CTGGCCAAGA AGGAAGAGAC GGAAAATAAA 
ATAAGGCAAA TGATCTACAC GCCGAAAGTA GTGGAAGAGC CAAAGGTAAA ACTCCCTGAC 
ATGAGACAAC AACAACCGAA TAACGAAAAA GAACAGGAAG ATATTAACAA CAATCACATG 
AAGGAATACG ATAAAGAAAG CAATATGACT TTGAGTAACA ACTCTTTGGA CGACGAACCC 
TCTGATGAGG AACCCAATAT TATTCCTTGG AGGGCACAAT TACGAAAAAC AAACAGTTCT 
TTGAATCTTC TCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109546548 ( XM_019917540.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773099.1 Dendroctonus ponderosae espin (LOC109546548), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe35527
Clone ID Related Accession (Same CDS sequence)	XM_019917540.1
Accession Version	XM_019917540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	espin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851160.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCGT TGGAAAAAGG TGCCGCGACC GCAGTGGGGG AACCACATGG TCCTCCCAAC 
GGAAATGCCA CTTTGGCGCT TCATTATGCC GCCGCTAGAG GATGCTTGGA CTGCGTTCGA 
CTTTTGGTGG ATTCCTCAGT CGAATTAAGC GCTAATACTC AGATGGACAA TGACGTGACA 
CCAGTTTATT TAGCTGCCCA AGAAGGCCAT CTCGATGTCC TCAAGTTTCT CGTTTTAGAA 
GCAGGAGGTT CTCTATACGT GAGAGCCAAA GATGGAATGG CTCCTATTCA CGCTGCTAGC 
CAGATGGGAT GTTTAAATTG CGTTAAATGG ATGATTCAAG ATCAAGGTGT CGACCCTAAT 
CTGAGAGATG GAGATGGAGC GACTCCATTG CATTTTGCCG CCTCCAGGGG TCACTTGGAT 
ACTGTGAGAT GGTTGCTTAA ACACGGCGCA AGATTGAGCT TGGATAAGTT TGGAAAAAGC 
CCCATAAATG ATGCTGCTGA AAATCAGCAA GTAGAATGTC TGAACATCTT GGTGCAACAC 
GGTACCACTC CAGATTATAA CGATTCTGAT AGACCCAACA ACGGAAAAAG TTGTTCTTGT 
GCAAGAAAAG GAGATCACAT AAAACGAGGA AATAGCACAG GCTCTGATTG CTCAACCTGT 
AAGCCCAAAC AGGCTGTAGG TAACTCAAAA AACTCCAAAA GCTCTTTGGC ATCTCTGGAG 
CCTTTCTATT TACACCCTCC CCTCAATGGT AGGAGCATGG AACCAGCACA CGCTCCAGAG 
AATGGGCTTT ATATCAATCC GATGACCAAT CATAGACACA GTGCGTCAAC TTGCTCAGAT 
ACCAGCTCAA TAGGAAGTGA GTCTTTCTAC CTTCATAATC CCAGTGAAGT GAGCAAAGAA 
GTGGTGTACA ATCGAGTCAA GGGGTTGTTC GATTCGAATG GGGAGCAACG ACCAATGTCG 
GGATTAAAAG TGAAAGTTGA AGTTCATTCC TCATCATCCG GTGCAGGATC TGACGAAAAT 
CTGTCATCTT CGGATTTATC TTCAGTTCGA TCGAATGACC ACGAAAACGA CTATGAGGAC 
ATTTACCTCG TTAGAGAAGA AGCACGAAAT CAAGAAAGAA CTAACATTCA TAATATAAAT 
GGACGATCAC GATCCAGAGA CTCTGGTTCA CATAGCAGAT CAGGTTCGAT CAGCTCATCC 
AATTCAGGAT GTAACGTAAT AGTGAAAATG ACTCCCAATG GAAACACCAA GAACGGAATA 
TACAAATCTA ACGAGTTCCT ACCTAAACCT CAGAAAATTG AAAAGTTAAC CGACAGAAAA 
CCTATGCAAG ATAGAGAGCG AACTTCAAAC ATTTCATCAG TTCCGGTAGA GGCTTCGCCA 
CCTCCACCAC CACCTCCACC TCCGAACAAC CCCAGCTCAC TCATTGACCC CAATGACCCC 
CATTTGAATG AACCCAGGGG AGCTGAAATC GTCGAAGTGG TTGAAGAACC CACCCTTAGA 
CCTTCGGACA TTGTGAAGGG CATGTGCAGA AGTATGTCAG CTCTATCTGC TCGTCGGTTG 
AATTCTTCCT GTTCCGACCT CACAATCGTC GGTCTTAACG ACGAAGACAG ACAGTCAACA 
TCCAGCACTA TTCTAGCTAC ACCAGTGAAT CGTCAACGAG TTTTACCATT TGTGCCTCCC 
AGCTTTCCAC TAGCTGGCTC GAACAAGCTA ATCAAACCGT CTGAATTTCT TAGAAGTATC 
AGCTCAGCTG ACAAACGAAA TGATGGTCCC AGAGAAATAT CGATGAGTGA GGATGATAAA 
AAAGCTGATA TGGACAAGGA AGAAGAACAG ACCAATAGCG ACAGTAAAGT TGTTGGGCCT 
CCACCGCCAC CCTTGCCAGA GTTTAATGGC GATTTAACAC AAAACGCCAT TTCAAAACAA 
AGCACCTTAA CACACCAGGA GAAAGTAAAA AAGCATCAAC CTTTGTCAGC GATTAGCATC 
CAAGACCTGA ACTCGGTCCA GCTGAGAAGA ACTGAAAAAC TTGTTGCTTC TAAGACTTTC 
TCAGCACCCA CTAGAAGTGT ATCATTGCAA TGCCTCAATA CGGCTGATTC ATTCATGGCT 
CAGAAAACTG ACCTAATCGC CGAGCTCAAA ATGTCGAAGG ATTTAACTGG ACTGAAGAAA 
ATGAAGATCG AAAAAGCGAA AACGGAACAG ACATTGGAAA GAGAAGTAAT AGTTGAAATT 
CGAAAACAGT TTACTGCTAA CAACTTTATA GAGAAGGCAA GTATATACAA GAGCGGATTG 
AGTCGCCACG ATGCACCATT ATCAATTCCA GACAAAGACA ATACTGGAAA CCTAATTCCT 
GCTTGGAAAA GGCAAATGCT AGCTAAGAAA GCTGCAGAGA AGGCCAAGAA GGAAATGGAA 
GAACAGCTGC AAAAAGATGC TGAAGAAAAG CGTTTACAAT CCATCCCTCA ATGGAAACGA 
CCATTGCTGG CCAAGAAGGA AGAGACGGAA AATAAAATAA GGCAAATGAT CTACACGCCG 
AAAGTAGTGG AAGAGCCAAA GGTAAAACTC CCTGACATGA GACAACAACA ACCGAATAAC 
GAAAAAGAAC AGGAAGATAT TAACAACAAT CACATGAAGG AATACGATAA AGAAAGCAAT 
ATGACTTTGA GTAACAACTC TTTGGACGAC GAACCCTCTG ATGAGGAACC CAATATTATT 
CCTTGGAGGG CACAATTACG AAAAACAAAC AGTTCTTTGA ATCTTCTCTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773099.1
CDS	148..2901
Translation	MPALEKGAATAVGEPHGPPNGNATLALHYAAARGCLDCVRLLVDSSVELSANTQMDNDVTPVYLAAQEGHLDVLKFLVLEAGGSLYVRAKDGMAPIHAASQMGCLNCVKWMIQDQGVDPNLRDGDGATPLHFAASRGHLDTVRWLLKHGARLSLDKFGKSPINDAAENQQVECLNILVQHGTTPDYNDSDRPNNGKSCSCARKGDHIKRGNSTGSDCSTCKPKQAVGNSKNSKSSLASLEPFYLHPPLNGRSMEPAHAPENGLYINPMTNHRHSASTCSDTSSIGSESFYLHNPSEVSKEVVYNRVKGLFDSNGEQRPMSGLKVKVEVHSSSSGAGSDENLSSSDLSSVRSNDHENDYEDIYLVREEARNQERTNIHNINGRSRSRDSGSHSRSGSISSSNSGCNVIVKMTPNGNTKNGIYKSNEFLPKPQKIEKLTDRKPMQDRERTSNISSVPVEASPPPPPPPPPNNPSSLIDPNDPHLNEPRGAEIVEVVEEPTLRPSDIVKGMCRSMSALSARRLNSSCSDLTIVGLNDEDRQSTSSTILATPVNRQRVLPFVPPSFPLAGSNKLIKPSEFLRSISSADKRNDGPREISMSEDDKKADMDKEEEQTNSDSKVVGPPPPPLPEFNGDLTQNAISKQSTLTHQEKVKKHQPLSAISIQDLNSVQLRRTEKLVASKTFSAPTRSVSLQCLNTADSFMAQKTDLIAELKMSKDLTGLKKMKIEKAKTEQTLEREVIVEIRKQFTANNFIEKASIYKSGLSRHDAPLSIPDKDNTGNLIPAWKRQMLAKKAAEKAKKEMEEQLQKDAEEKRLQSIPQWKRPLLAKKEETENKIRQMIYTPKVVEEPKVKLPDMRQQQPNNEKEQEDINNNHMKEYDKESNMTLSNNSLDDEPSDEEPNIIPWRAQLRKTNSSLNLLC

Target ORF information:

RefSeq Version	XM_019917540.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae espin (LOC109546548), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917540.1

ORF Insert Sequence:

ATGCCCGCGT TGGAAAAAGG TGCCGCGACC GCAGTGGGGG AACCACATGG TCCTCCCAAC 
GGAAATGCCA CTTTGGCGCT TCATTATGCC GCCGCTAGAG GATGCTTGGA CTGCGTTCGA 
CTTTTGGTGG ATTCCTCAGT CGAATTAAGC GCTAATACTC AGATGGACAA TGACGTGACA 
CCAGTTTATT TAGCTGCCCA AGAAGGCCAT CTCGATGTCC TCAAGTTTCT CGTTTTAGAA 
GCAGGAGGTT CTCTATACGT GAGAGCCAAA GATGGAATGG CTCCTATTCA CGCTGCTAGC 
CAGATGGGAT GTTTAAATTG CGTTAAATGG ATGATTCAAG ATCAAGGTGT CGACCCTAAT 
CTGAGAGATG GAGATGGAGC GACTCCATTG CATTTTGCCG CCTCCAGGGG TCACTTGGAT 
ACTGTGAGAT GGTTGCTTAA ACACGGCGCA AGATTGAGCT TGGATAAGTT TGGAAAAAGC 
CCCATAAATG ATGCTGCTGA AAATCAGCAA GTAGAATGTC TGAACATCTT GGTGCAACAC 
GGTACCACTC CAGATTATAA CGATTCTGAT AGACCCAACA ACGGAAAAAG TTGTTCTTGT 
GCAAGAAAAG GAGATCACAT AAAACGAGGA AATAGCACAG GCTCTGATTG CTCAACCTGT 
AAGCCCAAAC AGGCTGTAGG TAACTCAAAA AACTCCAAAA GCTCTTTGGC ATCTCTGGAG 
CCTTTCTATT TACACCCTCC CCTCAATGGT AGGAGCATGG AACCAGCACA CGCTCCAGAG 
AATGGGCTTT ATATCAATCC GATGACCAAT CATAGACACA GTGCGTCAAC TTGCTCAGAT 
ACCAGCTCAA TAGGAAGTGA GTCTTTCTAC CTTCATAATC CCAGTGAAGT GAGCAAAGAA 
GTGGTGTACA ATCGAGTCAA GGGGTTGTTC GATTCGAATG GGGAGCAACG ACCAATGTCG 
GGATTAAAAG TGAAAGTTGA AGTTCATTCC TCATCATCCG GTGCAGGATC TGACGAAAAT 
CTGTCATCTT CGGATTTATC TTCAGTTCGA TCGAATGACC ACGAAAACGA CTATGAGGAC 
ATTTACCTCG TTAGAGAAGA AGCACGAAAT CAAGAAAGAA CTAACATTCA TAATATAAAT 
GGACGATCAC GATCCAGAGA CTCTGGTTCA CATAGCAGAT CAGGTTCGAT CAGCTCATCC 
AATTCAGGAT GTAACGTAAT AGTGAAAATG ACTCCCAATG GAAACACCAA GAACGGAATA 
TACAAATCTA ACGAGTTCCT ACCTAAACCT CAGAAAATTG AAAAGTTAAC CGACAGAAAA 
CCTATGCAAG ATAGAGAGCG AACTTCAAAC ATTTCATCAG TTCCGGTAGA GGCTTCGCCA 
CCTCCACCAC CACCTCCACC TCCGAACAAC CCCAGCTCAC TCATTGACCC CAATGACCCC 
CATTTGAATG AACCCAGGGG AGCTGAAATC GTCGAAGTGG TTGAAGAACC CACCCTTAGA 
CCTTCGGACA TTGTGAAGGG CATGTGCAGA AGTATGTCAG CTCTATCTGC TCGTCGGTTG 
AATTCTTCCT GTTCCGACCT CACAATCGTC GGTCTTAACG ACGAAGACAG ACAGTCAACA 
TCCAGCACTA TTCTAGCTAC ACCAGTGAAT CGTCAACGAG TTTTACCATT TGTGCCTCCC 
AGCTTTCCAC TAGCTGGCTC GAACAAGCTA ATCAAACCGT CTGAATTTCT TAGAAGTATC 
AGCTCAGCTG ACAAACGAAA TGATGGTCCC AGAGAAATAT CGATGAGTGA GGATGATAAA 
AAAGCTGATA TGGACAAGGA AGAAGAACAG ACCAATAGCG ACAGTAAAGT TGTTGGGCCT 
CCACCGCCAC CCTTGCCAGA GTTTAATGGC GATTTAACAC AAAACGCCAT TTCAAAACAA 
AGCACCTTAA CACACCAGGA GAAAGTAAAA AAGCATCAAC CTTTGTCAGC GATTAGCATC 
CAAGACCTGA ACTCGGTCCA GCTGAGAAGA ACTGAAAAAC TTGTTGCTTC TAAGACTTTC 
TCAGCACCCA CTAGAAGTGT ATCATTGCAA TGCCTCAATA CGGCTGATTC ATTCATGGCT 
CAGAAAACTG ACCTAATCGC CGAGCTCAAA ATGTCGAAGG ATTTAACTGG ACTGAAGAAA 
ATGAAGATCG AAAAAGCGAA AACGGAACAG ACATTGGAAA GAGAAGTAAT AGTTGAAATT 
CGAAAACAGT TTACTGCTAA CAACTTTATA GAGAAGGCAA GTATATACAA GAGCGGATTG 
AGTCGCCACG ATGCACCATT ATCAATTCCA GACAAAGACA ATACTGGAAA CCTAATTCCT 
GCTTGGAAAA GGCAAATGCT AGCTAAGAAA GCTGCAGAGA AGGCCAAGAA GGAAATGGAA 
GAACAGCTGC AAAAAGATGC TGAAGAAAAG CGTTTACAAT CCATCCCTCA ATGGAAACGA 
CCATTGCTGG CCAAGAAGGA AGAGACGGAA AATAAAATAA GGCAAATGAT CTACACGCCG 
AAAGTAGTGG AAGAGCCAAA GGTAAAACTC CCTGACATGA GACAACAACA ACCGAATAAC 
GAAAAAGAAC AGGAAGATAT TAACAACAAT CACATGAAGG AATACGATAA AGAAAGCAAT 
ATGACTTTGA GTAACAACTC TTTGGACGAC GAACCCTCTG ATGAGGAACC CAATATTATT 
CCTTGGAGGG CACAATTACG AAAAACAAAC AGTTCTTTGA ATCTTCTCTG TTAA