LOC109538948 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109538948
Entrez Gene ID	109538948
Full Name	probable ATP-dependent RNA helicase DDX46
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019906394.1	XP_019761953.1	probable ATP-dependent RNA helicase DDX46

Pathways from BioSystems
Gene Ontology

The following LOC109538948 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109538948 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe42776	XM_019906394.1 Latest version!	Dendroctonus ponderosae probable ATP-dependent RNA helicase DDX46 (LOC109538948), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109538948 ( XM_019906394.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019761953.1 Dendroctonus ponderosae probable ATP-dependent RNA helicase DDX46 (LOC109538948), mRNA.

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CloneID	ODe42776
Clone ID Related Accession (Same CDS sequence)	XM_019906394.1
Accession Version	XM_019906394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	probable ATP-dependent RNA helicase DDX46
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017852083.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGAA GCCCAGAGCG AAGATCTCGC CGATCTAGAT CCAGAAGTAA AGAACGGTCC 
ACTAAAAGTA GTCGTAGAAG GAGCAGGTCT CGAGATCGGG ACAGGCGAAG AAGTCGCTCA 
AAAGATAAGG TTACCAGTGA GAGAAATAGG TCAAGAGACA GGGGAAGAAA CAGGAGCCAG 
TCTCGGGATA GATTCAGGCG AGATCGGGAT AGAAGTAAAT CTAGAGATAG GGATAGAGAC 
AGGGGCAGGG ATCATAGAGA TAGAAGAGCT GGAAGTAAAG ACAGGAAAAA AAGAAAAAAC 
TCTAGATCGC GAACACCAGA AAAAAAAGAT GATTCGGGGT TGTCGTTTGA TCCTGCAAAC 
TTGGATAAGG AAGAAGAGCA AAAAAAACTG GAATTAGAGA TGCAAAAGCG GCGAGAACGA 
ATAGAGCGAT GGAGGGCTGA AAGAAAAAAA AAAGAGCAGG ACTCTGTGAA GAAGGAATCT 
AAATCGGATT CCTCAAATTC ATCACAAGGA GCAATAAAGA AATGGTCTTT AGAAGATGAT 
TCTGAAGATG AGGATAAAAT GGATGATAAG TCGGAGGTGA AAAAAGAGGA AGAAACCGTC 
AAGGAAGAAA ATGGAAAAGT CGAGGAGGTT GAGAAGAAAG AGGAGCCAGA AGAGAAACCC 
ACAATTGACA CTGAAAAAAT GGAAGAAGAT GATGAAGAAG AGGATGACGA TGACGTCGAT 
CCTCTGGACG CATTTATGCA AGGTGTACAA GAGGAAGTAA GAAACATTAA TAAAATCCAT 
GTGAAAAAGG GCAATAAAAA TGGGTCTTCC AAAAAAGGGT CACTTGTAAT TGTGACTGGG 
GTGGCAAAGA AAAAGGTAGA CAAAAATAAA GGAGAACTTA TTGAGCAAAA TCAAGATGGA 
TTGGAATATT CTTCAGAGGA AGAAGCAGAA GATTTGAAAG ATACAGCAGC GAATATAGCA 
AATAAACAAA GGAAAGATTT AGCTCGAATA GACCACAATA ATATTACATA TGTTTCATTT 
AGGAAGAATT TCTATGTAGA AGTTCCAGAA ATAGGCAAAA TGACTAATGT AGAGGTAGAT 
GCATATAAGG AAGAATTAGA AGGAATTAGA GTCAAAGGTA AAGGATGCCC AAAGCCAATA 
AAAACATGGG CGCAATGTGG AGTTTCAACT AAAGAGCTCA ATGTTTTAAA GAGGATGGGC 
TTCGACAAGC CTACGCCAAT TCAAACACAA GCGATTCCCG CTATAATGTC TGGAAGAGAT 
CTTATCGGCA TAGCGAAAAC TGGAAGCGGC AAAACTCTAG CTTTCCTGTT ACCGATGTTT 
CGACATATAC TCGATCAGCC TCCACTGGAG GAAACCGATG GGCCTATCGC AATAATCATG 
ACTCCCACTC GGGAGTTGTG CATGCAAATA GGCAAAGATA TCAAGAAGTT TACAAAAAGT 
TTAAATTTAT ATGCAGTGTG CGTGTATGGA GGTACGGGAA TATCCGAGCA AATTGCAGAA 
TTGAAGCGAG GTGCCGAGAT TATAGTCTGT ACCCCTGGCC GCATGATCGA TATGTTGGCA 
GCAAACAGTG GAAAAGTTAC AAATTTAAGA CGAGTTACTT ACATAGTTTT GGATGAAGCT 
GATAGAATGT TTGATATGGG ATTTGAACCG CAGGTAATGA GAATCATAGA CAATGTGCGC 
CCAGATAGGC AAACTGTTAT GTTTAGTGCA ACTTTTCCAA GGCAAATGGA AGCTCTGGCA 
CGCAGGATTT TAATGAAACC TATCGAGGTT CAAGTGGGAG GGCGATCAGT TGTTTGTCAA 
GAGGTTGAGC AACACGTAGT TGTATTAGAA GAAGAGCAAA AATTCTTGAA ACTTTTGGAG 
CTTTTAGGAC TTTTTCAAGA GCAAGGAAGT ATTATTATTT TTGTGGATAA GCAAGAAAAT 
GCGGATATTT TGCTGAAAGA ATTAATGAGG GCATCATATA ATTGCATGAG CTTACACGGA 
GGTATAGATC AGTTTGATAG GGATTCCACG ATTGTTGACT TCAAGTCTGG AAAAATAAAA 
TTATTAGTGG CCACTTCGGT AGCTGCAAGA GGTCTTGATG TGAAACAACT AATTTTGGTT 
GTTAATTACG ATTGCCCAAA CCATTATGAA GATTATGTGC ATAGATGTGG AAGAACGGGG 
AGGGCTGGAA ATAAAGGGTT CGCCTACACT TTTATTACTC CTGAACAGGG CAGATATGCG 
GGCGATATAA TTTGGGCCCT TGAGTTAGCA ACTCTCCATG TGCCTGAAAC CTTGAGGAAG 
TTGTGGGATG ATTACAAATC GAAATTAGAA TCAGAAGGAA AAAGAGTGCA TACTGGGGGT 
GGTTTTAGCG GCAAAGGTTT TAAGTTTGAC GAGAACGAAG CTGCAGCTGT AAATGAGCGG 
AAAAAGTTCC AGAAGGCTGC TTTAGGTTTG CAAGACAGCG ACGATGAAGA CTTGGAACAG 
GACATTGACC AGCAAATTGA AAGCATGTTT GCAACCAAAC GAACTGTAAA AGAAATTAAG 
GCTCCTCTCG GAATTAGCCC AGCAGCTGCA GCTGCTGCCG CCCAAGCGTC GGCGAACTCT 
AGCGGGTCAG CTGTTAATAA ACTGGAATTG GCTAAGAAGC TTGCGTCGAA AATCACGTTT 
ACGAAAAGTA CAAATTTTGA TGCCAAATTC GCTACGCAAC AAGCCGCTGA GTCTATTCTC 
AAAGGTAGCA GTTCTGCACT TCCAATAACA GCAAAGACGG TGGCTGAGCA GCTAGCGGCG 
AAACTGAACA ACAAATTGAA CTACCAGCCC AAAGACGATG AAGATCGTAT GGACGAAGAC 
ACCGAACAGA CGTTTAGAAA GTATGAAGAA GAGTTGGAAA TTAATGACTT CCCACAACAA 
GCCAGATGGA GAGTTACATC AAAGGAGGCA TTAGCACAAA TTTCTGAATA TTCAGAAGCC 
GGTATCACCG TGAGAGGTAC TTATGTTCCG ACTGGAAAAG CACCCCCAGA AGGGGAGCGC 
AAGTTATATC TTGCAATTGA AAGTACTTCA GATATGGCAG TTTCCAAAGC CAAATCGGAG 
ATCACCAGAT TAATCAAGGA GGAATTGCTC AAGCTGCAGA CGTCCGGGCA CCACATGATT 
AACAAAAATA GATACAAAGT AATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019761953.1
CDS	128..3274
Translation	MVRSPERRSRRSRSRSKERSTKSSRRRSRSRDRDRRRSRSKDKVTSERNRSRDRGRNRSQSRDRFRRDRDRSKSRDRDRDRGRDHRDRRAGSKDRKKRKNSRSRTPEKKDDSGLSFDPANLDKEEEQKKLELEMQKRRERIERWRAERKKKEQDSVKKESKSDSSNSSQGAIKKWSLEDDSEDEDKMDDKSEVKKEEETVKEENGKVEEVEKKEEPEEKPTIDTEKMEEDDEEEDDDDVDPLDAFMQGVQEEVRNINKIHVKKGNKNGSSKKGSLVIVTGVAKKKVDKNKGELIEQNQDGLEYSSEEEAEDLKDTAANIANKQRKDLARIDHNNITYVSFRKNFYVEVPEIGKMTNVEVDAYKEELEGIRVKGKGCPKPIKTWAQCGVSTKELNVLKRMGFDKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTLAFLLPMFRHILDQPPLEETDGPIAIIMTPTRELCMQIGKDIKKFTKSLNLYAVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGKVTNLRRVTYIVLDEADRMFDMGFEPQVMRIIDNVRPDRQTVMFSATFPRQMEALARRILMKPIEVQVGGRSVVCQEVEQHVVVLEEEQKFLKLLELLGLFQEQGSIIIFVDKQENADILLKELMRASYNCMSLHGGIDQFDRDSTIVDFKSGKIKLLVATSVAARGLDVKQLILVVNYDCPNHYEDYVHRCGRTGRAGNKGFAYTFITPEQGRYAGDIIWALELATLHVPETLRKLWDDYKSKLESEGKRVHTGGGFSGKGFKFDENEAAAVNERKKFQKAALGLQDSDDEDLEQDIDQQIESMFATKRTVKEIKAPLGISPAAAAAAAQASANSSGSAVNKLELAKKLASKITFTKSTNFDAKFATQQAAESILKGSSSALPITAKTVAEQLAAKLNNKLNYQPKDDEDRMDEDTEQTFRKYEEELEINDFPQQARWRVTSKEALAQISEYSEAGITVRGTYVPTGKAPPEGERKLYLAIESTSDMAVSKAKSEITRLIKEELLKLQTSGHHMINKNRYKVI

Target ORF information:

RefSeq Version	XM_019906394.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae probable ATP-dependent RNA helicase DDX46 (LOC109538948), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019906394.1

ORF Insert Sequence:

ATGGTTAGAA GCCCAGAGCG AAGATCTCGC CGATCTAGAT CCAGAAGTAA AGAACGGTCC 
ACTAAAAGTA GTCGTAGAAG GAGCAGGTCT CGAGATCGGG ACAGGCGAAG AAGTCGCTCA 
AAAGATAAGG TTACCAGTGA GAGAAATAGG TCAAGAGACA GGGGAAGAAA CAGGAGCCAG 
TCTCGGGATA GATTCAGGCG AGATCGGGAT AGAAGTAAAT CTAGAGATAG GGATAGAGAC 
AGGGGCAGGG ATCATAGAGA TAGAAGAGCT GGAAGTAAAG ACAGGAAAAA AAGAAAAAAC 
TCTAGATCGC GAACACCAGA AAAAAAAGAT GATTCGGGGT TGTCGTTTGA TCCTGCAAAC 
TTGGATAAGG AAGAAGAGCA AAAAAAACTG GAATTAGAGA TGCAAAAGCG GCGAGAACGA 
ATAGAGCGAT GGAGGGCTGA AAGAAAAAAA AAAGAGCAGG ACTCTGTGAA GAAGGAATCT 
AAATCGGATT CCTCAAATTC ATCACAAGGA GCAATAAAGA AATGGTCTTT AGAAGATGAT 
TCTGAAGATG AGGATAAAAT GGATGATAAG TCGGAGGTGA AAAAAGAGGA AGAAACCGTC 
AAGGAAGAAA ATGGAAAAGT CGAGGAGGTT GAGAAGAAAG AGGAGCCAGA AGAGAAACCC 
ACAATTGACA CTGAAAAAAT GGAAGAAGAT GATGAAGAAG AGGATGACGA TGACGTCGAT 
CCTCTGGACG CATTTATGCA AGGTGTACAA GAGGAAGTAA GAAACATTAA TAAAATCCAT 
GTGAAAAAGG GCAATAAAAA TGGGTCTTCC AAAAAAGGGT CACTTGTAAT TGTGACTGGG 
GTGGCAAAGA AAAAGGTAGA CAAAAATAAA GGAGAACTTA TTGAGCAAAA TCAAGATGGA 
TTGGAATATT CTTCAGAGGA AGAAGCAGAA GATTTGAAAG ATACAGCAGC GAATATAGCA 
AATAAACAAA GGAAAGATTT AGCTCGAATA GACCACAATA ATATTACATA TGTTTCATTT 
AGGAAGAATT TCTATGTAGA AGTTCCAGAA ATAGGCAAAA TGACTAATGT AGAGGTAGAT 
GCATATAAGG AAGAATTAGA AGGAATTAGA GTCAAAGGTA AAGGATGCCC AAAGCCAATA 
AAAACATGGG CGCAATGTGG AGTTTCAACT AAAGAGCTCA ATGTTTTAAA GAGGATGGGC 
TTCGACAAGC CTACGCCAAT TCAAACACAA GCGATTCCCG CTATAATGTC TGGAAGAGAT 
CTTATCGGCA TAGCGAAAAC TGGAAGCGGC AAAACTCTAG CTTTCCTGTT ACCGATGTTT 
CGACATATAC TCGATCAGCC TCCACTGGAG GAAACCGATG GGCCTATCGC AATAATCATG 
ACTCCCACTC GGGAGTTGTG CATGCAAATA GGCAAAGATA TCAAGAAGTT TACAAAAAGT 
TTAAATTTAT ATGCAGTGTG CGTGTATGGA GGTACGGGAA TATCCGAGCA AATTGCAGAA 
TTGAAGCGAG GTGCCGAGAT TATAGTCTGT ACCCCTGGCC GCATGATCGA TATGTTGGCA 
GCAAACAGTG GAAAAGTTAC AAATTTAAGA CGAGTTACTT ACATAGTTTT GGATGAAGCT 
GATAGAATGT TTGATATGGG ATTTGAACCG CAGGTAATGA GAATCATAGA CAATGTGCGC 
CCAGATAGGC AAACTGTTAT GTTTAGTGCA ACTTTTCCAA GGCAAATGGA AGCTCTGGCA 
CGCAGGATTT TAATGAAACC TATCGAGGTT CAAGTGGGAG GGCGATCAGT TGTTTGTCAA 
GAGGTTGAGC AACACGTAGT TGTATTAGAA GAAGAGCAAA AATTCTTGAA ACTTTTGGAG 
CTTTTAGGAC TTTTTCAAGA GCAAGGAAGT ATTATTATTT TTGTGGATAA GCAAGAAAAT 
GCGGATATTT TGCTGAAAGA ATTAATGAGG GCATCATATA ATTGCATGAG CTTACACGGA 
GGTATAGATC AGTTTGATAG GGATTCCACG ATTGTTGACT TCAAGTCTGG AAAAATAAAA 
TTATTAGTGG CCACTTCGGT AGCTGCAAGA GGTCTTGATG TGAAACAACT AATTTTGGTT 
GTTAATTACG ATTGCCCAAA CCATTATGAA GATTATGTGC ATAGATGTGG AAGAACGGGG 
AGGGCTGGAA ATAAAGGGTT CGCCTACACT TTTATTACTC CTGAACAGGG CAGATATGCG 
GGCGATATAA TTTGGGCCCT TGAGTTAGCA ACTCTCCATG TGCCTGAAAC CTTGAGGAAG 
TTGTGGGATG ATTACAAATC GAAATTAGAA TCAGAAGGAA AAAGAGTGCA TACTGGGGGT 
GGTTTTAGCG GCAAAGGTTT TAAGTTTGAC GAGAACGAAG CTGCAGCTGT AAATGAGCGG 
AAAAAGTTCC AGAAGGCTGC TTTAGGTTTG CAAGACAGCG ACGATGAAGA CTTGGAACAG 
GACATTGACC AGCAAATTGA AAGCATGTTT GCAACCAAAC GAACTGTAAA AGAAATTAAG 
GCTCCTCTCG GAATTAGCCC AGCAGCTGCA GCTGCTGCCG CCCAAGCGTC GGCGAACTCT 
AGCGGGTCAG CTGTTAATAA ACTGGAATTG GCTAAGAAGC TTGCGTCGAA AATCACGTTT 
ACGAAAAGTA CAAATTTTGA TGCCAAATTC GCTACGCAAC AAGCCGCTGA GTCTATTCTC 
AAAGGTAGCA GTTCTGCACT TCCAATAACA GCAAAGACGG TGGCTGAGCA GCTAGCGGCG 
AAACTGAACA ACAAATTGAA CTACCAGCCC AAAGACGATG AAGATCGTAT GGACGAAGAC 
ACCGAACAGA CGTTTAGAAA GTATGAAGAA GAGTTGGAAA TTAATGACTT CCCACAACAA 
GCCAGATGGA GAGTTACATC AAAGGAGGCA TTAGCACAAA TTTCTGAATA TTCAGAAGCC 
GGTATCACCG TGAGAGGTAC TTATGTTCCG ACTGGAAAAG CACCCCCAGA AGGGGAGCGC 
AAGTTATATC TTGCAATTGA AAGTACTTCA GATATGGCAG TTTCCAAAGC CAAATCGGAG 
ATCACCAGAT TAATCAAGGA GGAATTGCTC AAGCTGCAGA CGTCCGGGCA CCACATGATT 
AACAAAAATA GATACAAAGT AATTTAA