sema4e cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema4e
Entrez Gene ID	796377
Full Name	semaphorin 4e
Synonyms	sema7,semaZ7
General protein information	Preferred Names semaphorin 4e Names semaphorin-4E sema Z7 semaphorin-7 semaphorin-Z7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168429.4	XP_005168486.1	semaphorin-4E isoform X1
XM_017359054.2	XP_017214543.1	semaphorin-4E isoform X1
XM_005168429.3	XP_005168486.1	semaphorin-4E isoform X1
XM_017359054.1	XP_017214543.1	semaphorin-4E isoform X1
XM_021481386.1	XP_021337061.1	semaphorin-4E isoform X1
NM_131049.1	NP_571124.1	semaphorin-4E isoform 1 precursor
NM_001083865.1	NP_001077334.1	semaphorin-4E isoform 2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	sema4e	NP_571124.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006636	XP_316667.4
Danio rerio (zebrafish)	si:ch211-129c21.1	NP_001038473.1
Drosophila melanogaster (fruit fly)	Sema-2b	NP_001261024.1
Caenorhabditis elegans (roundworm)	mab-20	NP_490875.1

Gene Ontology
Bibliography

Related articles in PubMed

Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos.
Wu Y, Lou QY, Ge F, Xiong Q
Scientific reports7(1)4022(2017 Jun)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Gene-expression analysis of hair cell regeneration in the zebrafish lateral line.
Jiang L, Romero-Carvajal A, Haug JS, Seidel CW, Piotrowski T
Proceedings of the National Academy of Sciences of the United States of America111(14)E1383-92(2014 Apr)

Cellular and molecular bases of axonal pathfinding during embryogenesis of the fish central nervous system.
Bernhardt RR
Journal of neurobiology38(1)137-60(1999 Jan)

Molecular cloning and expression of two novel zebrafish semaphorins.
Halloran MC, Severance SM, Yee CS, Gemza DL, Kuwada JY
Mechanisms of development76(1-2)165-8(1998 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sema4e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema4e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38386	NM_001083865.1 Latest version!	Danio rerio semaphorin 4e (sema4e), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa38516	NM_131049.1 Latest version!	Danio rerio semaphorin 4e (sema4e), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07507	XM_017359054.2 Latest version!	Danio rerio semaphorin 4e (sema4e), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07507	XM_005168429.4 Latest version!	Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07507	XM_021481386.1 Latest version!	Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa07507	XM_005168429.3	Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa07507	XM_017359054.1	Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema4e ( NM_001083865.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077334.1 Danio rerio semaphorin 4e (sema4e), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa38386
Clone ID Related Accession (Same CDS sequence)	NM_001083865.1
Accession Version	NM_001083865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	semaphorin-4E isoform 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CU458994.1. On or before Apr 5, 2007 this sequence version replaced XM_001333508.1, XM_001333586.1. ##Evidence-Data-START## Transcript exon combination :: CU458994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGATCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ATCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GTCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATGCACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGGACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGCTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTTTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CAGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTACTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTCGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGACAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAACG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGCGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAACCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCCATCAGT 
GAATGTGGGC AGTACCAGAC GTGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACC ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AGAGCTTGAG TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCATGGG AATACCGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAACACC 
ATCCAGATCC TCTCCGATGG CTTGATGATC TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTTA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAGGAC 
ACACAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCA 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077334.1
CDS	1..2301
Translation	MVSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESCTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARLDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHARQKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGQYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLSDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	NM_001083865.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083865.1

ORF Insert Sequence:

ATGGTGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGATCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ATCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GTCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATGCACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGGACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGCTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTTTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CAGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTACTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTCGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGACAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAACG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGCGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAACCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCCATCAGT 
GAATGTGGGC AGTACCAGAC GTGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACC ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AGAGCTTGAG TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCATGGG AATACCGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAACACC 
ATCCAGATCC TCTCCGATGG CTTGATGATC TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTTA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAGGAC 
ACACAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCA 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( NM_131049.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571124.1 Danio rerio semaphorin 4e (sema4e), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa38516
Clone ID Related Accession (Same CDS sequence)	NM_131049.1
Accession Version	NM_131049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Danio rerio(zebrafish)
Product	semaphorin-4E isoform 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF073289.1. ##Evidence-Data-START## Transcript exon combination :: AF073289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA4476728 [ECO:0000350] ##Evidence-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTTG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGAACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGGACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGCTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTTTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CAGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGATCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCCATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AGAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACCGTAA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTAA ATGGCAGTTT AACGGGGAGC CAATCAAACC ATCAAACACC 
ATCCAGATCC TCTCCGATGG CTTGATGATC TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACACCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGCC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571124.1
CDS	545..2845
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKNDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARLDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFDPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVKWQFNGEPIKPSNTIQILSDGLMIFNASLDATGYYTCSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKATQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	NM_131049.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131049.1

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTTG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGAACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGGACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGCTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTTTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CAGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGATCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCCATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AGAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACCGTAA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTAA ATGGCAGTTT AACGGGGAGC CAATCAAACC ATCAAACACC 
ATCCAGATCC TCTCCGATGG CTTGATGATC TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACACCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGCC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( XM_005168429.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168486.1 Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07507
Clone ID Related Accession (Same CDS sequence)	XM_005168429.4 , XM_017359054.2 , XM_005168429.3 , XM_017359054.1 , XM_021481386.1
Accession Version	XM_005168429.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-4E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168429.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168486.1
CDS	534..2834
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARFDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	XM_005168429.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168429.4

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( XM_017359054.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214543.1 Danio rerio semaphorin 4e (sema4e), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa07507
Clone ID Related Accession (Same CDS sequence)	XM_005168429.4 , XM_017359054.2 , XM_005168429.3 , XM_017359054.1 , XM_021481386.1
Accession Version	XM_017359054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-4E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214543.1
CDS	513..2813
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARFDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	XM_017359054.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359054.2

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( XM_005168429.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168486.1 Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07507
Clone ID Related Accession (Same CDS sequence)	XM_005168429.4 , XM_017359054.2 , XM_005168429.3 , XM_017359054.1 , XM_021481386.1
Accession Version	XM_005168429.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-4E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168486.1
CDS	519..2819
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARFDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	XM_005168429.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168429.3

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( XM_017359054.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214543.1 Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07507
Clone ID Related Accession (Same CDS sequence)	XM_005168429.4 , XM_017359054.2 , XM_005168429.3 , XM_017359054.1 , XM_021481386.1
Accession Version	XM_017359054.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-4E isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214543.1
CDS	513..2813
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARFDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	XM_017359054.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359054.1

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema4e ( XM_021481386.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337061.1 Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07507
Clone ID Related Accession (Same CDS sequence)	XM_005168429.4 , XM_017359054.2 , XM_005168429.3 , XM_017359054.1 , XM_021481386.1
Accession Version	XM_021481386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-4E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337061.1
CDS	475..2775
Translation	MMSLLAVLCVLYVWSPAMLTGGLGSTLDSLPRKTVPIGSNGGRLFREEGIWNYTTMLLRDDLNLLILGAREAIFALDLDDITIKKAMLKWEVTRDQQNDCSNKGKDATNDCKNYIRILHKKDDGRMYVCGTKAFNPTCGYLSYADGKLTLEILQEDTKGKCPFDPFQRYTSAMVDGAYYSATSMNFRGSEPVMMRSTEESIRTEFTSTWLSEPNFIHMAHIPEGQSNPDGDDDKIYLFFSETAVEYESYTKVDVSRVARVCKGDLGGQRTLQKKWTSFLKARFDCQVPNTNLPLLVQDVFHLCPDDWTTCVFYAVFTPQSDSSQYSAVCSYKIEDIKTVFSKGKFKAPFNVETSFVKWVMYSGELPDPRPGACIDNHAREKGITKSLELPDKTLQFVKDKPLMDQAVTAEQPLLVKRGAAFTRIVVTTATALNGTSHQVMFIGTKSGSVLKAVNYNGEMVIMEEIQLFEPSEPIKILRLSSSKKQLYVGSEVGVVQLSISECGRYQTCLDCVLARDPHCGWDLDTEHCATINSIHRTRSSTVIQSLNDGDASQCPAIGVSKPVNISFYHGNTVKLGCQPYSNLAQVEWQLNGEPIKPSNTIQILSDGLMIFNASLDATGYYICSSIETVSQRKYQTQHVAYDVKMWSESGTTASLHHVKEKERTLVAMVVILSLVLAALLIWNLYKGHLSLPCLHRRVKDTQNRHEDDANQVQPQRLASSVVNFNSNNNHANDQRYSSSRETDRLSTTAGSTGQMSLKYIDDESEI

Target ORF information:

RefSeq Version	XM_021481386.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio semaphorin 4e (sema4e), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481386.1

ORF Insert Sequence:

ATGATGTCTC TGCTGGCTGT TCTGTGTGTT TTGTATGTCT GGAGTCCAGC AATGCTGACC 
GGTGGGCTGG GATCAACACT GGATAGCCTA CCTCGGAAAA CAGTGCCAAT TGGAAGTAAT 
GGAGGACGTT TGTTTCGTGA AGAGGGAATT TGGAACTACA CCACCATGCT TCTGAGGGAT 
GATCTCAACC TGCTCATCCT GGGTGCCAGA GAAGCCATTT TTGCACTTGA CCTCGATGAC 
ATTACAATCA AGAAGGCCAT GTTGAAATGG GAAGTAACAC GTGACCAGCA AAACGACTGT 
TCAAATAAGG GGAAAGATGC TACGAATGAC TGCAAAAACT ATATTCGAAT TCTTCATAAA 
AAGGACGACG GCAGAATGTA TGTCTGTGGA ACCAAAGCGT TCAACCCCAC CTGTGGCTAT 
TTGTCCTATG CGGATGGTAA GCTGACTTTA GAGATCCTCC AAGAAGATAC GAAAGGGAAA 
TGTCCGTTCG ACCCTTTTCA GCGATATACA TCTGCAATGG TCGATGGAGC GTATTACTCT 
GCTACTTCAA TGAACTTTCG GGGTTCAGAG CCGGTGATGA TGCGCAGCAC AGAGGAAAGC 
ATTCGAACTG AGTTTACAAG CACCTGGCTC AGTGAACCAA ATTTTATCCA CATGGCCCAC 
ATACCAGAGG GCCAATCTAA TCCAGATGGG GATGATGACA AGATCTATCT GTTCTTCAGT 
GAGACGGCAG TGGAATATGA GTCATACACA AAGGTGGACG TGTCTCGAGT GGCTCGTGTG 
TGCAAGGGTG ACTTGGGTGG GCAGAGAACG CTACAAAAGA AGTGGACGTC ATTTCTAAAA 
GCTAGGTTCG ACTGTCAAGT TCCAAACACC AATCTCCCTC TTCTGGTGCA GGATGTTTTC 
CACTTGTGTC CAGATGACTG GACCACCTGT GTTTTTTATG CTGTCTTTAC CCCACAATCA 
GACTCCTCTC AGTATTCTGC TGTATGTTCA TACAAAATTG AAGACATCAA GACCGTGTTC 
TCGAAGGGCA AATTTAAGGC TCCGTTTAAT GTGGAGACGT CATTTGTGAA GTGGGTGATG 
TACTCAGGCG AGCTTCCTGA TCCCCGACCC GGAGCGTGCA TTGACAATCA TGCCAGAGAA 
AAGGGCATCA CCAAATCTCT GGAGCTTCCA GACAAAACGT TACAGTTTGT CAAAGACAAG 
CCTCTGATGG ACCAGGCCGT CACTGCAGAG CAACCCCTGC TGGTGAAGAG AGGAGCTGCG 
TTCACTAGAA TAGTAGTGAC CACCGCGACC GCCTTGAATG GGACCAGCCA TCAAGTCATG 
TTCATCGGCA CAAAGAGTGG ATCAGTCCTG AAAGCTGTGA ATTATAATGG AGAGATGGTC 
ATTATGGAAG AGATTCAGCT ATTTGAGCCC TCGGAGCCCA TCAAGATACT GCGTCTTTCA 
AGCAGCAAGA AGCAGCTGTA TGTGGGTTCG GAGGTGGGCG TGGTTCAGCT GTCAATCAGT 
GAATGTGGGC GGTACCAGAC ATGTCTGGAC TGTGTTCTGG CCAGGGATCC GCACTGTGGC 
TGGGACCTCG ATACTGAGCA CTGTGCTACT ATTAACAGCA TCCACAGAAC CAGAAGCAGC 
ACTGTGATTC AAAGCTTAAA TGATGGAGAT GCCAGTCAAT GTCCAGCAAT AGGTGTCAGT 
AAACCAGTCA ACATCTCCTT TTACCACGGG AATACTGTGA AGCTGGGCTG CCAGCCTTAT 
TCCAACCTGG CGCAGGTTGA ATGGCAGTTA AACGGGGAGC CAATCAAACC ATCAAATACC 
ATCCAGATCC TCTCCGATGG CTTGATGATA TTCAACGCAT CTTTGGACGC CACCGGCTAC 
TACATCTGCA GCTCCATCGA GACCGTCTCC CAGCGCAAAT ACCAGACTCA GCATGTAGCG 
TATGATGTAA AGATGTGGTC CGAATCTGGA ACCACAGCGT CACTTCATCA TGTTAAGGAA 
AAAGAGCGCA CCCTAGTGGC CATGGTGGTG ATACTGTCAC TAGTTCTAGC TGCATTGTTG 
ATATGGAACC TGTACAAAGG CCATTTGTCT CTGCCATGTT TACACAGGAG GGTAAAAGAC 
ACGCAAAACA GGCACGAAGA CGATGCTAAC CAGGTTCAGC CACAGAGACT AGCATCATCG 
GTAGTGAACT TCAACAGCAA CAACAACCAC GCCAATGACC AGAGGTATTC CAGCTCTAGA 
GAAACAGACA GACTCTCGAC CACTGCTGGG TCCACAGGTC AGATGTCACT GAAATACATA 
GATGATGAAT CAGAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos.
Scientific reports7(1)4022(2017 Jun)
Wu Y,Lou QY,Ge F,Xiong Q

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Gene-expression analysis of hair cell regeneration in the zebrafish lateral line.
Proceedings of the National Academy of Sciences of the United States of America111(14)E1383-92(2014 Apr)
Jiang L,Romero-Carvajal A,Haug JS,Seidel CW,Piotrowski T

Cellular and molecular bases of axonal pathfinding during embryogenesis of the fish central nervous system.
Journal of neurobiology38(1)137-60(1999 Jan)
Bernhardt RR

Molecular cloning and expression of two novel zebrafish semaphorins.
Mechanisms of development76(1-2)165-8(1998 Aug)
Halloran MC,Severance SM,Yee CS,Gemza DL,Kuwada JY

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sema4e cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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