suz12a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	suz12a
Entrez Gene ID	794171
Full Name	SUZ12 polycomb repressive complex 2a subunit
Synonyms	suz12,suz12l,zgc:100863
General protein information	Preferred Names SUZ12 polycomb repressive complex 2a subunit Names polycomb protein suz12-A suppressor of zeste 12 homolog a suppressor of zeste 12 homolog, like suppressor of zeste 12 protein homolog A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021471664.1	XP_021327339.1	polycomb protein suz12-A isoform X1
NM_001003529.2	NP_001003529.1	polycomb protein suz12-A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
Gagnon JA, Valen E, Thyme SB, Huang P, Akhmetova L, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF
PloS one9(5)e98186(2014)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following suz12a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the suz12a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03847	NM_001003529.2 Latest version!	Danio rerio SUZ12 polycomb repressive complex 2 subunit a (suz12a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa08477	XM_021471664.1 Latest version!	Danio rerio SUZ12 polycomb repressive complex 2a subunit (suz12a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

suz12a ( NM_001003529.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003529.1 Danio rerio SUZ12 polycomb repressive complex 2 subunit a (suz12a), mRNA.

Price & Availability↑

CloneID	ODa03847
Clone ID Related Accession (Same CDS sequence)	NM_001003529.2
Accession Version	NM_001003529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-25
Organism	Danio rerio(zebrafish)
Product	polycomb protein suz12-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078293.1. On Aug 30, 2012 this sequence version replaced NM_001003529.1. ##Evidence-Data-START## Transcript exon combination :: BC078293.1, BC152625.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCCAC AAAAGCACGG CTCGGGTGGG AATGGTTTTT ATGGCAACAG CGCGAGTAAA 
GCCGCTAATG GAGGAGCTCA CGCATCGTCC GGTGTTGTGA TGACTTCAGT CAAGCGACTC 
AAGATGGAGC AGATCCAAGC CGACCACCAA CTCTTCCTGG AGGCGTTCGA GAAACCAACT 
CAAATATACA GATTTCTTCG CACAAGGAAT CTCATAACTC CAATTTTCCT GCACCGATCT 
CTTACATATA TGTCCCACAG AAATTCGCGA ACTAATTCAA AAAGAAAGAG CTTCAGTTTG 
AATGATCTGC TGTTTAAAGT GGAGAAGTCA AAAGTGGATC CAGAATCTCA CAATTTGGCT 
TCAAACCTGC AACTCACTTT CACTGGATTC TTCCATAAAA CTGAGAAGCA GTTGCAGAAC 
TCAGAGAATG AAGAAAACTC TGTGTCTGTA GAAGTGCTAC TCGTCAAGCT CTGCCACAAG 
AAAAGAAAGG ATGTGACTTG TCCAGTGAAG CAAGTGCCTA CGGGTAAAAA GCAGGTTCCC 
CTTAACCCAG ACATCAGTCC TACCAAGCTG GGAGCTTTTC CCACTCTGGT AGTGCCCAGC 
CACGAGTTTG AGCCTGTTAA CAGCAACACG GTGAAGTCCT ACTCCCTTCT GTTTAGAGCG 
TCTCGTCCAT GGGGAAGAGA GCACAATGGC ATGACCACCA GAGATACCAA TGTTATTGAA 
GAGCTGTCTA ACAGGAAGAA GAGATACTGC TCTCATCAGG ATGATGGAGA AACTACATTT 
GTGGCACAAA TGACTGTTTT TGATAAAAAT AGACGCCTCC AGCTTTTGGA TGGTGAATAT 
GAAGTCTCCA TGCAGGAGAT AGAGGAGAGC CCTGTAGGCA AGAAAAGGGC GACTTGGGAA 
ACAATATTAG ATGAGAAGTG GGTTCCTCCC TTCGAGACCT TTTCTCAGGG ACCAACACTG 
CAGTTCACTT TGCGATGGAC TAATGACACA GCGGACAAAG CGACAGCACC AGTTGCTAAA 
CCGCTGGCCA CACGCAATTC TGAGTCCAGC ACAGTAGACA GCAGTAAAAC TAGTAATATA 
AAGCCACCAC AGGCTGTAGC AGTTAATGAT TCTTTAGGCA CTGATCTACC AGTTAGAAGA 
GAACAAACTC ACATTGAACC TTGTCAGAAA CTACATGTTT ATTATCAGTT CCTGTATAAC 
AATAACACAC GGCAGCAGAC AGAGGCCAGA GATGACCTGC ACTGTCCCTG GTGCACGCTG 
AACTGCCGTA AGCTCTACAG CCTCCTTAAG CACCTCAAGC TCTCGCACAG CCGCTTCATC 
TTCAACTATG TGCCTCATCC TAAAGGAGCC AGGATAGATG TGTCAATCAA TGAATGCTAT 
GACGGGTCTT ATGTGGGCAA CCCTCAGGAC ATCCTCTGCC AGCCTGGCTT TGCCTTTAGC 
CGTAATGGTC CAGTAAAGAG GACTCCAGTT ACACAGATCC TTGTCTGCAG GCCTAAGCGC 
TCAAAGCCCA GCCTTTCTGA GTTCCTGGAG CCGGAGGATG GAGAGCAGGA GCAGCAGCGA 
ACGTACATCA GTGGCCACAA CCGCCTGTAC TTCCACAGTG ACAGCTGCAC GCCACTTCGA 
CCACAGGAAA TGGAAGTGGA CAGTGAGGAC GAGAGAGACC CGGATTGGCT CAGGGAGAAG 
ACTGCTATGC AAATTGAAGA GTTCACAGAT GTTAACGAAG GAGAGAAAGA GATCATGAAA 
CTGTGGAACC TGCTCGTGAT GAAACATGGA TTCATAGCTG ACAACCAAAT GAATCAGGCC 
TGTATGTCGT TTGTGGAGCA GCACGGCACC ATCATGGTGG AGAAGAATCT GTGCCGAAAC 
GCCCTGCTCC ATCTCATCAA TATGCACGAC TTCGGCTTGA TAACCACAGC AACCATCGAC 
AAGGCCATGA CTCACCTGAG AGATCTCACA CAGCAATCCA TAGCGCAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003529.1
CDS	228..2201
Translation	MAPQKHGSGGNGFYGNSASKAANGGAHASSGVVMTSVKRLKMEQIQADHQLFLEAFEKPTQIYRFLRTRNLITPIFLHRSLTYMSHRNSRTNSKRKSFSLNDLLFKVEKSKVDPESHNLASNLQLTFTGFFHKTEKQLQNSENEENSVSVEVLLVKLCHKKRKDVTCPVKQVPTGKKQVPLNPDISPTKLGAFPTLVVPSHEFEPVNSNTVKSYSLLFRASRPWGREHNGMTTRDTNVIEELSNRKKRYCSHQDDGETTFVAQMTVFDKNRRLQLLDGEYEVSMQEIEESPVGKKRATWETILDEKWVPPFETFSQGPTLQFTLRWTNDTADKATAPVAKPLATRNSESSTVDSSKTSNIKPPQAVAVNDSLGTDLPVRREQTHIEPCQKLHVYYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLSHSRFIFNYVPHPKGARIDVSINECYDGSYVGNPQDILCQPGFAFSRNGPVKRTPVTQILVCRPKRSKPSLSEFLEPEDGEQEQQRTYISGHNRLYFHSDSCTPLRPQEMEVDSEDERDPDWLREKTAMQIEEFTDVNEGEKEIMKLWNLLVMKHGFIADNQMNQACMSFVEQHGTIMVEKNLCRNALLHLINMHDFGLITTATIDKAMTHLRDLTQQSIAQH

Target ORF information:

RefSeq Version	NM_001003529.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SUZ12 polycomb repressive complex 2 subunit a (suz12a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003529.2

ORF Insert Sequence:

ATGGCTCCAC AAAAGCACGG CTCGGGTGGG AATGGTTTTT ATGGCAACAG CGCGAGTAAA 
GCCGCTAATG GAGGAGCTCA CGCATCGTCC GGTGTTGTGA TGACTTCAGT CAAGCGACTC 
AAGATGGAGC AGATCCAAGC CGACCACCAA CTCTTCCTGG AGGCGTTCGA GAAACCAACT 
CAAATATACA GATTTCTTCG CACAAGGAAT CTCATAACTC CAATTTTCCT GCACCGATCT 
CTTACATATA TGTCCCACAG AAATTCGCGA ACTAATTCAA AAAGAAAGAG CTTCAGTTTG 
AATGATCTGC TGTTTAAAGT GGAGAAGTCA AAAGTGGATC CAGAATCTCA CAATTTGGCT 
TCAAACCTGC AACTCACTTT CACTGGATTC TTCCATAAAA CTGAGAAGCA GTTGCAGAAC 
TCAGAGAATG AAGAAAACTC TGTGTCTGTA GAAGTGCTAC TCGTCAAGCT CTGCCACAAG 
AAAAGAAAGG ATGTGACTTG TCCAGTGAAG CAAGTGCCTA CGGGTAAAAA GCAGGTTCCC 
CTTAACCCAG ACATCAGTCC TACCAAGCTG GGAGCTTTTC CCACTCTGGT AGTGCCCAGC 
CACGAGTTTG AGCCTGTTAA CAGCAACACG GTGAAGTCCT ACTCCCTTCT GTTTAGAGCG 
TCTCGTCCAT GGGGAAGAGA GCACAATGGC ATGACCACCA GAGATACCAA TGTTATTGAA 
GAGCTGTCTA ACAGGAAGAA GAGATACTGC TCTCATCAGG ATGATGGAGA AACTACATTT 
GTGGCACAAA TGACTGTTTT TGATAAAAAT AGACGCCTCC AGCTTTTGGA TGGTGAATAT 
GAAGTCTCCA TGCAGGAGAT AGAGGAGAGC CCTGTAGGCA AGAAAAGGGC GACTTGGGAA 
ACAATATTAG ATGAGAAGTG GGTTCCTCCC TTCGAGACCT TTTCTCAGGG ACCAACACTG 
CAGTTCACTT TGCGATGGAC TAATGACACA GCGGACAAAG CGACAGCACC AGTTGCTAAA 
CCGCTGGCCA CACGCAATTC TGAGTCCAGC ACAGTAGACA GCAGTAAAAC TAGTAATATA 
AAGCCACCAC AGGCTGTAGC AGTTAATGAT TCTTTAGGCA CTGATCTACC AGTTAGAAGA 
GAACAAACTC ACATTGAACC TTGTCAGAAA CTACATGTTT ATTATCAGTT CCTGTATAAC 
AATAACACAC GGCAGCAGAC AGAGGCCAGA GATGACCTGC ACTGTCCCTG GTGCACGCTG 
AACTGCCGTA AGCTCTACAG CCTCCTTAAG CACCTCAAGC TCTCGCACAG CCGCTTCATC 
TTCAACTATG TGCCTCATCC TAAAGGAGCC AGGATAGATG TGTCAATCAA TGAATGCTAT 
GACGGGTCTT ATGTGGGCAA CCCTCAGGAC ATCCTCTGCC AGCCTGGCTT TGCCTTTAGC 
CGTAATGGTC CAGTAAAGAG GACTCCAGTT ACACAGATCC TTGTCTGCAG GCCTAAGCGC 
TCAAAGCCCA GCCTTTCTGA GTTCCTGGAG CCGGAGGATG GAGAGCAGGA GCAGCAGCGA 
ACGTACATCA GTGGCCACAA CCGCCTGTAC TTCCACAGTG ACAGCTGCAC GCCACTTCGA 
CCACAGGAAA TGGAAGTGGA CAGTGAGGAC GAGAGAGACC CGGATTGGCT CAGGGAGAAG 
ACTGCTATGC AAATTGAAGA GTTCACAGAT GTTAACGAAG GAGAGAAAGA GATCATGAAA 
CTGTGGAACC TGCTCGTGAT GAAACATGGA TTCATAGCTG ACAACCAAAT GAATCAGGCC 
TGTATGTCGT TTGTGGAGCA GCACGGCACC ATCATGGTGG AGAAGAATCT GTGCCGAAAC 
GCCCTGCTCC ATCTCATCAA TATGCACGAC TTCGGCTTGA TAACCACAGC AACCATCGAC 
AAGGCCATGA CTCACCTGAG AGATCTCACA CAGCAATCCA TAGCGCAACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

suz12a ( XM_021471664.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327339.1 Danio rerio SUZ12 polycomb repressive complex 2a subunit (suz12a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08477
Clone ID Related Accession (Same CDS sequence)	XM_021471664.1
Accession Version	XM_021471664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	polycomb protein suz12-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCAC AAAAGCACGG CTCGGGTGGG AATGGTTTTT ATGGCAACAG CGCGAGTAAA 
GCCGCTAATG GAGGAGCTCA CGCATCGTCC GGTGTTGTGA TGACTTCAGT CAAGCGACTC 
AAGATGGAGC AGATCCAAGC CGACCACCAA CTCTTCCTGG AGGCGTTCGA GAAACCAACT 
CAAATATACA GATTTCTTCG CACAAGGAAT CTCATAACTC CAATTTTCCT GCACCGATCT 
CTTACATATA TGTCCCACAG AAACTCGCGA ACTAATTCAA AAAGAAAGAG CTTCAGTTTG 
AATGATCTGC TGTTTAAAGT GGAGAAGTCA AAAGTGGATC CAGAATCTCA CAATTTGGCT 
TCAAACCTTC AACTCACTTT CACTGGATTC TTCCATAAAA CTGAGAAGCA GTTGCAGAAC 
TCAGAGAATG AAGAAAACTC TGTGTCTGTA GAAGTGCTAC TCGTCAAGCT CTGCCACAAG 
AAAAGAAAGG ATGTGACTTG TCCAGTGAAG CAAGTGCCTA CGGGTAAAAA GCAGGTTCCC 
CTCAACCCAG ACATCAGTCC TACCAAGCTG GGAGCTTTTC CCACTCTGGT AGTGCCCAGC 
CACGAGTTTG AGCCTGTTAA CAGCAACACG GTGAAGTCCT ACTCCCTTCT GTTTAGAGCG 
TCTCGTCCAT GGGGAAGAGA GCCCAATGGC ATGACCACCA GAGATACCAA TGTTATTGAA 
GAGCTATCTA ACAGGAAGAA GAGATACTGC TCTCATCAGG ATGATGGAGA AACTACATTT 
GTGGCACAAA TGACTGTTTT TGATAAAAAT AGACGCCTCC AGCTTTTGGA TGGTGAATAT 
GAAGTCTCCA TGCAGGAGAT AGAGGAGAGC CCTGTAGGCA AGAAAAGGGC GACTTGGGAA 
ACAATATTAG ATGAGAAGTG GGTTCCTCCC TTCGAGACCT TTTCTCAGGG ACCAACACTG 
CAGTTCACTT TGCGATGGAC TAATGACACA GCGGACAAAG CGACAGCACC AGTTGCTAAA 
CCGCTGGCCA CACGCAATTC TGAGTCCAGC ACAGTAGACA GCAGTAAAAC TAGTAATGTA 
AAGCCACCAC AGGCTGTAGT TAATGATTCT TTAGGCACTG ATCTACCAGT TAGAAGAGAA 
CAAACTCACA TTGAACCTTG TCAGAAACTA CATGTTTATT ATCAGTTCCT GTATAACAAT 
AACACACGGC AGCAGACAGA GGCCAGAGAT GACCTGCACT GTCCCTGGTG CACGCTGAAC 
TGCCGTAAGC TCTACAGCCT CCTTAAGCAC CTCAAGCTCT CGCACAGCCG CTTCATCTTC 
AACTATGTGC CTCATCCTAA AGGAGCCAGG ATAGATGTGT CAATCAATGA ATGCTATGAC 
GGGTCTTATG TGGGCAACCC TCAGGACATC CTCTGCCAGC CTGGCTTTGC CTTTAGCCGT 
AATGGTCCAG TAAAGAGGAC TCCAGTTACA CAGATCCTTG TCTGCAGGCC TAAGCGCTCA 
AAGCCCAGCC TTTCTGAGTT CCTGGAGCCG GAGGATGGAG AGCAGGAGCA GCAGCGAACG 
TACATCAGTG GCCACAACCG CCTGTACTTC CACAGTGACA GCTGCACGCC ACTTCGACCA 
CAGGAAATGG AAGTGGACAG TGAGGACGAG AGAGACCCGG ATTGGCTCAG GGAGAAGACT 
GCTATGCAAA TTGAGGAGTT CACAGATGTT AACGAAGGAG AGAAAGAGAT CATGAAACTG 
TGGAACCTGC TCGTGATGAA ACATGGATTC ATAGCTGACA ACCAAATGAA TCAGGCCTGT 
ATGTCGTTTG TGGAGCAGCA CGGCACCATC ATGGTGGAGA AGAATCTGTG CCGAAACGCC 
CTGCTACATC TCATCAATAT GCACGACTTC GGCTTGATAA CCACAGCAAC CATCGACAAG 
GCCATGACTC ACCTGAGAGA TCTCACACAG CAATCCATAG CGCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327339.1
CDS	1243..3213
Translation	MAPQKHGSGGNGFYGNSASKAANGGAHASSGVVMTSVKRLKMEQIQADHQLFLEAFEKPTQIYRFLRTRNLITPIFLHRSLTYMSHRNSRTNSKRKSFSLNDLLFKVEKSKVDPESHNLASNLQLTFTGFFHKTEKQLQNSENEENSVSVEVLLVKLCHKKRKDVTCPVKQVPTGKKQVPLNPDISPTKLGAFPTLVVPSHEFEPVNSNTVKSYSLLFRASRPWGREPNGMTTRDTNVIEELSNRKKRYCSHQDDGETTFVAQMTVFDKNRRLQLLDGEYEVSMQEIEESPVGKKRATWETILDEKWVPPFETFSQGPTLQFTLRWTNDTADKATAPVAKPLATRNSESSTVDSSKTSNVKPPQAVVNDSLGTDLPVRREQTHIEPCQKLHVYYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLSHSRFIFNYVPHPKGARIDVSINECYDGSYVGNPQDILCQPGFAFSRNGPVKRTPVTQILVCRPKRSKPSLSEFLEPEDGEQEQQRTYISGHNRLYFHSDSCTPLRPQEMEVDSEDERDPDWLREKTAMQIEEFTDVNEGEKEIMKLWNLLVMKHGFIADNQMNQACMSFVEQHGTIMVEKNLCRNALLHLINMHDFGLITTATIDKAMTHLRDLTQQSIAQD

Target ORF information:

RefSeq Version	XM_021471664.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SUZ12 polycomb repressive complex 2a subunit (suz12a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471664.1

ORF Insert Sequence:

ATGGCTCCAC AAAAGCACGG CTCGGGTGGG AATGGTTTTT ATGGCAACAG CGCGAGTAAA 
GCCGCTAATG GAGGAGCTCA CGCATCGTCC GGTGTTGTGA TGACTTCAGT CAAGCGACTC 
AAGATGGAGC AGATCCAAGC CGACCACCAA CTCTTCCTGG AGGCGTTCGA GAAACCAACT 
CAAATATACA GATTTCTTCG CACAAGGAAT CTCATAACTC CAATTTTCCT GCACCGATCT 
CTTACATATA TGTCCCACAG AAACTCGCGA ACTAATTCAA AAAGAAAGAG CTTCAGTTTG 
AATGATCTGC TGTTTAAAGT GGAGAAGTCA AAAGTGGATC CAGAATCTCA CAATTTGGCT 
TCAAACCTTC AACTCACTTT CACTGGATTC TTCCATAAAA CTGAGAAGCA GTTGCAGAAC 
TCAGAGAATG AAGAAAACTC TGTGTCTGTA GAAGTGCTAC TCGTCAAGCT CTGCCACAAG 
AAAAGAAAGG ATGTGACTTG TCCAGTGAAG CAAGTGCCTA CGGGTAAAAA GCAGGTTCCC 
CTCAACCCAG ACATCAGTCC TACCAAGCTG GGAGCTTTTC CCACTCTGGT AGTGCCCAGC 
CACGAGTTTG AGCCTGTTAA CAGCAACACG GTGAAGTCCT ACTCCCTTCT GTTTAGAGCG 
TCTCGTCCAT GGGGAAGAGA GCCCAATGGC ATGACCACCA GAGATACCAA TGTTATTGAA 
GAGCTATCTA ACAGGAAGAA GAGATACTGC TCTCATCAGG ATGATGGAGA AACTACATTT 
GTGGCACAAA TGACTGTTTT TGATAAAAAT AGACGCCTCC AGCTTTTGGA TGGTGAATAT 
GAAGTCTCCA TGCAGGAGAT AGAGGAGAGC CCTGTAGGCA AGAAAAGGGC GACTTGGGAA 
ACAATATTAG ATGAGAAGTG GGTTCCTCCC TTCGAGACCT TTTCTCAGGG ACCAACACTG 
CAGTTCACTT TGCGATGGAC TAATGACACA GCGGACAAAG CGACAGCACC AGTTGCTAAA 
CCGCTGGCCA CACGCAATTC TGAGTCCAGC ACAGTAGACA GCAGTAAAAC TAGTAATGTA 
AAGCCACCAC AGGCTGTAGT TAATGATTCT TTAGGCACTG ATCTACCAGT TAGAAGAGAA 
CAAACTCACA TTGAACCTTG TCAGAAACTA CATGTTTATT ATCAGTTCCT GTATAACAAT 
AACACACGGC AGCAGACAGA GGCCAGAGAT GACCTGCACT GTCCCTGGTG CACGCTGAAC 
TGCCGTAAGC TCTACAGCCT CCTTAAGCAC CTCAAGCTCT CGCACAGCCG CTTCATCTTC 
AACTATGTGC CTCATCCTAA AGGAGCCAGG ATAGATGTGT CAATCAATGA ATGCTATGAC 
GGGTCTTATG TGGGCAACCC TCAGGACATC CTCTGCCAGC CTGGCTTTGC CTTTAGCCGT 
AATGGTCCAG TAAAGAGGAC TCCAGTTACA CAGATCCTTG TCTGCAGGCC TAAGCGCTCA 
AAGCCCAGCC TTTCTGAGTT CCTGGAGCCG GAGGATGGAG AGCAGGAGCA GCAGCGAACG 
TACATCAGTG GCCACAACCG CCTGTACTTC CACAGTGACA GCTGCACGCC ACTTCGACCA 
CAGGAAATGG AAGTGGACAG TGAGGACGAG AGAGACCCGG ATTGGCTCAG GGAGAAGACT 
GCTATGCAAA TTGAGGAGTT CACAGATGTT AACGAAGGAG AGAAAGAGAT CATGAAACTG 
TGGAACCTGC TCGTGATGAA ACATGGATTC ATAGCTGACA ACCAAATGAA TCAGGCCTGT 
ATGTCGTTTG TGGAGCAGCA CGGCACCATC ATGGTGGAGA AGAATCTGTG CCGAAACGCC 
CTGCTACATC TCATCAATAT GCACGACTTC GGCTTGATAA CCACAGCAAC CATCGACAAG 
GCCATGACTC ACCTGAGAGA TCTCACACAG CAATCCATAG CGCAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
PloS one9(5)e98186(2014)
Gagnon JA,Valen E,Thyme SB,Huang P,Akhmetova L,Ahkmetova L,Pauli A,Montague TG,Zimmerman S,Richter C,Schier AF

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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suz12a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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