crfb2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crfb2
Entrez Gene ID	777653
Full Name	cytokine receptor family member b2
General protein information	Preferred Names cytokine receptor family member b2 Names cytokine receptor family member b2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077626.2	NP_001071094.2	cytokine receptor family member b2 precursor
XM_005167960.3	XP_005168017.1	cytokine receptor family member b2 isoform X1
XM_005167960.4	XP_005168017.1	cytokine receptor family member b2 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Chen H, Liu W, Wang B, Mao H, Sun Z, Hou Q, Mi Y, Fan L, Hu C
Fish & shellfish immunology45(2)211-20(2015 Aug)

Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals.
Sun B, Greiner-Tollersrud L, Koop BF, Robertsen B
Developmental and comparative immunology47(2)275-86(2014 Dec)

Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production.
Li Y, Esain V, Teng L, Xu J, Kwan W, Frost IM, Yzaguirre AD, Cai X, Cortes M, Maijenburg MW, Tober J, Dzierzak E, Orkin SH, Tan K, North TE, Speck NA
Genes & development28(23)2597-612(2014 Dec)

Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
Palha N, Guivel-Benhassine F, Briolat V, Lutfalla G, Sourisseau M, Ellett F, Wang CH, Lieschke GJ, Herbomel P, Schwartz O, Levraud JP
PLoS pathogens9(9)e1003619(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crfb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crfb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15032	XM_005167960.4 Latest version!	Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa53225	NM_001077626.2 Latest version!	Danio rerio cytokine receptor family member b2 (crfb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa15032	XM_005167960.3	Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crfb2 ( XM_005167960.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168017.1 Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15032
Clone ID Related Accession (Same CDS sequence)	XM_005167960.3 , XM_005167960.4
Accession Version	XM_005167960.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cytokine receptor family member b2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167960.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGTTTAC TGTGGATAAG 
GAAGGGTTGG GGAATGAACC CCTTAGCAAC CTGGCCCCTG GCCAAAGGTA CTGTGTGACT 
GTCAGTATCA TTGATCGAAG CCCACCCAAC AGACCACCAG TATGTGCCTC AACCCCTAAA 
ACAGCAAATG TTTCAGATGC AGTTATCTCT GTCATTCTTT GTCTCCTGAT GGTGATATTC 
GTGATGTGCA CTCCGAGACT TGTTGTTCAC TTCTTTTGTC TAAAAGCAGA TCTTCCAGCA 
GTTCTGGGCT CGTATAAGGG TATAAACAAT GTTATTTTTA TCCCTACTGG TGAACCCATC 
AGTGCCCTCT ATGAGGAGAA AGGCAGACTG GAAAAAGAGA TTGAGGGAGA GGCAGAGGAG 
GAACTGAAAT ATGAAAAACT TGTGGACCTG TATCAGGATT TTCAGGGTTC CTCTCTCGCT 
ATTTTTCCAG TGTCATCTAG TTCTTGTTTG CTTATGAATC AAAGTCCAGA TAAACAGCTG 
TGCCCCACCT CTTCCATGAA TGCTCAGAAT AATGGTTTGA TTATGTACGA AGGCAGTCAT 
GTCCATACTG AGCCAGCTGA ACCAACAGCC TCTCCTAGGT GTGAACACCT TGCTCAAGGA 
ACAACTCAAC TTCCGAACTT ATGTTCTGTT GTCCCCCTTG CTTTGGAAAA GCAGGACAGT 
GAAAACAGTA CAGATGTAAA CCTGTTTTCT GTGAGGCTAG GAGGATTTTT AGCAGAGAAA 
CACAAAGTTG ATCCTGATAA AACCTGCAGG ACTAAAGATG AGCTGGGATC ATATCCTCAG 
AGCCCAGTAG AAACTGCTGT AATGGGACCT ACCTGGATTT CTGATGTACA GTCCCAAAAC 
TCTGAAGATG AGGATGAATA TTCAGATTAT TTAAGTCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168017.1
CDS	295..1719
Translation	MRFRHCTMSVITSILAFTVLFTLVLCMEIPAPEHLNLSSQYFVHLLSWKMGPGSPDGVHYSVKIKTKSGSMVVVEACENVTSPLLCNLTEAFSDVEEIYYIIVSAALGSHMSPNASYTPFKPIDNTILEPPLVTITVCNQSLCVSLRAPAERLSEIYKSFKLKYRLRVSSEDGTEFTVDKEGLGNEPLSNLAPGQRYCVTVSIIDRSPPNRPPVCASTPKTANVSDAVISVILCLLMVIFVMCTPRLVVHFFCLKADLPAVLGSYKGINNVIFIPTGEPISALYEEKGRLEKEIEGEAEEELKYEKLVDLYQDFQGSSLAIFPVSSSSCLLMNQSPDKQLCPTSSMNAQNNGLIMYEGSHVHTEPAEPTASPRCEHLAQGTTQLPNLCSVVPLALEKQDSENSTDVNLFSVRLGGFLAEKHKVDPDKTCRTKDELGSYPQSPVETAVMGPTWISDVQSQNSEDEDEYSDYLSQN

Target ORF information:

RefSeq Version	XM_005167960.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167960.3

ORF Insert Sequence:

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGTTTAC TGTGGATAAG 
GAAGGGTTGG GGAATGAACC CCTTAGCAAC CTGGCCCCTG GCCAAAGGTA CTGTGTGACT 
GTCAGTATCA TTGATCGAAG CCCACCCAAC AGACCACCAG TATGTGCCTC AACCCCTAAA 
ACAGCAAATG TTTCAGATGC AGTTATCTCT GTCATTCTTT GTCTCCTGAT GGTGATATTC 
GTGATGTGCA CTCCGAGACT TGTTGTTCAC TTCTTTTGTC TAAAAGCAGA TCTTCCAGCA 
GTTCTGGGCT CGTATAAGGG TATAAACAAT GTTATTTTTA TCCCTACTGG TGAACCCATC 
AGTGCCCTCT ATGAGGAGAA AGGCAGACTG GAAAAAGAGA TTGAGGGAGA GGCAGAGGAG 
GAACTGAAAT ATGAAAAACT TGTGGACCTG TATCAGGATT TTCAGGGTTC CTCTCTCGCT 
ATTTTTCCAG TGTCATCTAG TTCTTGTTTG CTTATGAATC AAAGTCCAGA TAAACAGCTG 
TGCCCCACCT CTTCCATGAA TGCTCAGAAT AATGGTTTGA TTATGTACGA AGGCAGTCAT 
GTCCATACTG AGCCAGCTGA ACCAACAGCC TCTCCTAGGT GTGAACACCT TGCTCAAGGA 
ACAACTCAAC TTCCGAACTT ATGTTCTGTT GTCCCCCTTG CTTTGGAAAA GCAGGACAGT 
GAAAACAGTA CAGATGTAAA CCTGTTTTCT GTGAGGCTAG GAGGATTTTT AGCAGAGAAA 
CACAAAGTTG ATCCTGATAA AACCTGCAGG ACTAAAGATG AGCTGGGATC ATATCCTCAG 
AGCCCAGTAG AAACTGCTGT AATGGGACCT ACCTGGATTT CTGATGTACA GTCCCAAAAC 
TCTGAAGATG AGGATGAATA TTCAGATTAT TTAAGTCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crfb2 ( XM_005167960.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168017.1 Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15032
Clone ID Related Accession (Same CDS sequence)	XM_005167960.3 , XM_005167960.4
Accession Version	XM_005167960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cytokine receptor family member b2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGTTTAC TGTGGATAAG 
GAAGGGTTGG GGAATGAACC CCTTAGCAAC CTGGCCCCTG GCCAAAGGTA CTGTGTGACT 
GTCAGTATCA TTGATCGAAG CCCACCCAAC AGACCACCAG TATGTGCCTC AACCCCTAAA 
ACAGCAAATG TTTCAGATGC AGTTATCTCT GTCATTCTTT GTCTCCTGAT GGTGATATTC 
GTGATGTGCA CTCCGAGACT TGTTGTTCAC TTCTTTTGTC TAAAAGCAGA TCTTCCAGCA 
GTTCTGGGCT CGTATAAGGG TATAAACAAT GTTATTTTTA TCCCTACTGG TGAACCCATC 
AGTGCCCTCT ATGAGGAGAA AGGCAGACTG GAAAAAGAGA TTGAGGGAGA GGCAGAGGAG 
GAACTGAAAT ATGAAAAACT TGTGGACCTG TATCAGGATT TTCAGGGTTC CTCTCTCGCT 
ATTTTTCCAG TGTCATCTAG TTCTTGTTTG CTTATGAATC AAAGTCCAGA TAAACAGCTG 
TGCCCCACCT CTTCCATGAA TGCTCAGAAT AATGGTTTGA TTATGTACGA AGGCAGTCAT 
GTCCATACTG AGCCAGCTGA ACCAACAGCC TCTCCTAGGT GTGAACACCT TGCTCAAGGA 
ACAACTCAAC TTCCGAACTT ATGTTCTGTT GTCCCCCTTG CTTTGGAAAA GCAGGACAGT 
GAAAACAGTA CAGATGTAAA CCTGTTTTCT GTGAGGCTAG GAGGATTTTT AGCAGAGAAA 
CACAAAGTTG ATCCTGATAA AACCTGCAGG ACTAAAGATG AGCTGGGATC ATATCCTCAG 
AGCCCAGTAG AAACTGCTGT AATGGGACCT ACCTGGATTT CTGATGTACA GTCCCAAAAC 
TCTGAAGATG AGGATGAATA TTCAGATTAT TTAAGTCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168017.1
CDS	290..1714
Translation	MRFRHCTMSVITSILAFTVLFTLVLCMEIPAPEHLNLSSQYFVHLLSWKMGPGSPDGVHYSVKIKTKSGSMVVVEACENVTSPLLCNLTEAFSDVEEIYYIIVSAALGSHMSPNASYTPFKPIDNTILEPPLVTITVCNQSLCVSLRAPAERLSEIYKSFKLKYRLRVSSEDGTEFTVDKEGLGNEPLSNLAPGQRYCVTVSIIDRSPPNRPPVCASTPKTANVSDAVISVILCLLMVIFVMCTPRLVVHFFCLKADLPAVLGSYKGINNVIFIPTGEPISALYEEKGRLEKEIEGEAEEELKYEKLVDLYQDFQGSSLAIFPVSSSSCLLMNQSPDKQLCPTSSMNAQNNGLIMYEGSHVHTEPAEPTASPRCEHLAQGTTQLPNLCSVVPLALEKQDSENSTDVNLFSVRLGGFLAEKHKVDPDKTCRTKDELGSYPQSPVETAVMGPTWISDVQSQNSEDEDEYSDYLSQN

Target ORF information:

RefSeq Version	XM_005167960.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytokine receptor family member b2 (crfb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167960.4

ORF Insert Sequence:

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGTTTAC TGTGGATAAG 
GAAGGGTTGG GGAATGAACC CCTTAGCAAC CTGGCCCCTG GCCAAAGGTA CTGTGTGACT 
GTCAGTATCA TTGATCGAAG CCCACCCAAC AGACCACCAG TATGTGCCTC AACCCCTAAA 
ACAGCAAATG TTTCAGATGC AGTTATCTCT GTCATTCTTT GTCTCCTGAT GGTGATATTC 
GTGATGTGCA CTCCGAGACT TGTTGTTCAC TTCTTTTGTC TAAAAGCAGA TCTTCCAGCA 
GTTCTGGGCT CGTATAAGGG TATAAACAAT GTTATTTTTA TCCCTACTGG TGAACCCATC 
AGTGCCCTCT ATGAGGAGAA AGGCAGACTG GAAAAAGAGA TTGAGGGAGA GGCAGAGGAG 
GAACTGAAAT ATGAAAAACT TGTGGACCTG TATCAGGATT TTCAGGGTTC CTCTCTCGCT 
ATTTTTCCAG TGTCATCTAG TTCTTGTTTG CTTATGAATC AAAGTCCAGA TAAACAGCTG 
TGCCCCACCT CTTCCATGAA TGCTCAGAAT AATGGTTTGA TTATGTACGA AGGCAGTCAT 
GTCCATACTG AGCCAGCTGA ACCAACAGCC TCTCCTAGGT GTGAACACCT TGCTCAAGGA 
ACAACTCAAC TTCCGAACTT ATGTTCTGTT GTCCCCCTTG CTTTGGAAAA GCAGGACAGT 
GAAAACAGTA CAGATGTAAA CCTGTTTTCT GTGAGGCTAG GAGGATTTTT AGCAGAGAAA 
CACAAAGTTG ATCCTGATAA AACCTGCAGG ACTAAAGATG AGCTGGGATC ATATCCTCAG 
AGCCCAGTAG AAACTGCTGT AATGGGACCT ACCTGGATTT CTGATGTACA GTCCCAAAAC 
TCTGAAGATG AGGATGAATA TTCAGATTAT TTAAGTCAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crfb2 ( NM_001077626.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071094.2 Danio rerio cytokine receptor family member b2 (crfb2), mRNA.

Price & Availability↑

CloneID	ODa53225
Clone ID Related Accession (Same CDS sequence)	NM_001077626.2
Accession Version	NM_001077626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	cytokine receptor family member b2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR361551.8. On Nov 6, 2015 this sequence version replaced NM_001077626.1. ##Evidence-Data-START## Transcript exon combination :: EF014953.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGGAAGG GTTGGGGAAT 
GAACCCCTTA GCAACCTGGC CCCTGGCCAA AGGTACTGTG TGACTGTCAG TATCATTGAT 
CGAAGCCCAC CCAACAGACC ACCAGTATGT GCCTCAACCC CTAAAACAGC AAATGTTTCA 
GATGCAGTTA TCTCTGTCAT TCTTTGTCTC CTGATGGTGA TATTCGTGAT GTGCACTCCG 
AGACTTGTTG TTCACTTCTT TTGTCTAAAA GCAGATCTTC CAGCAGTTCT GGGCTCGTAT 
AAGGGTATAA ACAATGTTAT TTTTATCCCT ACTGGTGAAC CCATCAGTGC CCTCTATGAG 
GAGAAAGGCA GACTGGAAAA AGAGATTGAG GGAGAGGCAG AGGAGGAACT GAAATATGAA 
AAACTTGTGG ACCTGTATCA GGATTTTCAG GGTTCCTCTC TCGCTATTTT TCCAGTGTCA 
TCTAGTTCTT GTTTGCTTAT GAATCAAAGT CCAGATAAAC AGCTGTGCCC CACCTCTTCC 
ATGAATGCTC AGAATAATGG TTTGATTATG TACGAAGGCA GTCATGTCCA TACTGAGCCA 
GCTGAACCAA CAGCCTCTCC TAGGTGTGAA CACCTTGCTC AAGGAACAAC TCAACTTCCG 
AACTTATGTT CTGTTGTCCC CCTTGCTTTG GAAAAGCAGG ACAGTGAAAA CAGTACAGAT 
GTAAACCTGT TTTCTGTGAG GCTAGGAGGA TTTTTAGCAG AGAAACACAA AGTTGATCCT 
GATAAAACCT GCAGGACTAA AGATGAGCTG GGATCATATC CTCAGAGCCC AGTAGAAACT 
GCTGTAATGG GACCTACCTG GATTTCTGAT GTACAGTCCC AAAACTCTGA AGATGAGGAT 
GAATATTCAG ATTATTTAAG TCAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071094.2
CDS	195..1604
Translation	MRFRHCTMSVITSILAFTVLFTLVLCMEIPAPEHLNLSSQYFVHLLSWKMGPGSPDGVHYSVKIKTKSGSMVVVEACENVTSPLLCNLTEAFSDVEEIYYIIVSAALGSHMSPNASYTPFKPIDNTILEPPLVTITVCNQSLCVSLRAPAERLSEIYKSFKLKYRLRVSSEDGTEEGLGNEPLSNLAPGQRYCVTVSIIDRSPPNRPPVCASTPKTANVSDAVISVILCLLMVIFVMCTPRLVVHFFCLKADLPAVLGSYKGINNVIFIPTGEPISALYEEKGRLEKEIEGEAEEELKYEKLVDLYQDFQGSSLAIFPVSSSSCLLMNQSPDKQLCPTSSMNAQNNGLIMYEGSHVHTEPAEPTASPRCEHLAQGTTQLPNLCSVVPLALEKQDSENSTDVNLFSVRLGGFLAEKHKVDPDKTCRTKDELGSYPQSPVETAVMGPTWISDVQSQNSEDEDEYSDYLSQN

Target ORF information:

RefSeq Version	NM_001077626.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cytokine receptor family member b2 (crfb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077626.2

ORF Insert Sequence:

ATGCGATTCC GACATTGCAC AATGTCTGTG ATTACAAGTA TACTTGCATT CACTGTTCTG 
TTTACCCTAG TGCTATGCAT GGAAATACCT GCCCCGGAAC ATCTAAACCT GTCGTCCCAA 
TATTTTGTCC ACTTGTTGTC TTGGAAAATG GGGCCAGGCT CTCCTGATGG TGTCCACTAC 
TCCGTCAAAA TAAAAACAAA AAGTGGGAGT ATGGTGGTGG TGGAGGCCTG TGAAAATGTG 
ACATCCCCTC TACTGTGTAA TCTTACAGAA GCCTTTTCTG ATGTGGAGGA AATCTATTAC 
ATTATTGTTT CTGCCGCTCT AGGCAGCCAT ATGTCACCGA ATGCGTCCTA TACCCCATTC 
AAACCCATTG ATAACACTAT TTTAGAGCCA CCGCTGGTGA CAATCACAGT CTGTAATCAA 
TCACTGTGTG TAAGTCTCAG AGCACCTGCT GAGAGGCTCT CTGAAATTTA CAAGTCATTC 
AAGTTGAAGT ATAGACTGAG GGTCAGCAGT GAAGATGGAA CAGAGGAAGG GTTGGGGAAT 
GAACCCCTTA GCAACCTGGC CCCTGGCCAA AGGTACTGTG TGACTGTCAG TATCATTGAT 
CGAAGCCCAC CCAACAGACC ACCAGTATGT GCCTCAACCC CTAAAACAGC AAATGTTTCA 
GATGCAGTTA TCTCTGTCAT TCTTTGTCTC CTGATGGTGA TATTCGTGAT GTGCACTCCG 
AGACTTGTTG TTCACTTCTT TTGTCTAAAA GCAGATCTTC CAGCAGTTCT GGGCTCGTAT 
AAGGGTATAA ACAATGTTAT TTTTATCCCT ACTGGTGAAC CCATCAGTGC CCTCTATGAG 
GAGAAAGGCA GACTGGAAAA AGAGATTGAG GGAGAGGCAG AGGAGGAACT GAAATATGAA 
AAACTTGTGG ACCTGTATCA GGATTTTCAG GGTTCCTCTC TCGCTATTTT TCCAGTGTCA 
TCTAGTTCTT GTTTGCTTAT GAATCAAAGT CCAGATAAAC AGCTGTGCCC CACCTCTTCC 
ATGAATGCTC AGAATAATGG TTTGATTATG TACGAAGGCA GTCATGTCCA TACTGAGCCA 
GCTGAACCAA CAGCCTCTCC TAGGTGTGAA CACCTTGCTC AAGGAACAAC TCAACTTCCG 
AACTTATGTT CTGTTGTCCC CCTTGCTTTG GAAAAGCAGG ACAGTGAAAA CAGTACAGAT 
GTAAACCTGT TTTCTGTGAG GCTAGGAGGA TTTTTAGCAG AGAAACACAA AGTTGATCCT 
GATAAAACCT GCAGGACTAA AGATGAGCTG GGATCATATC CTCAGAGCCC AGTAGAAACT 
GCTGTAATGG GACCTACCTG GATTTCTGAT GTACAGTCCC AAAACTCTGA AGATGAGGAT 
GAATATTCAG ATTATTTAAG TCAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella).
Fish & shellfish immunology45(2)211-20(2015 Aug)
Chen H,Liu W,Wang B,Mao H,Sun Z,Hou Q,Mi Y,Fan L,Hu C

Atlantic salmon possesses two clusters of type I interferon receptor genes on different chromosomes, which allows for a larger repertoire of interferon receptors than in zebrafish and mammals.
Developmental and comparative immunology47(2)275-86(2014 Dec)
Sun B,Greiner-Tollersrud L,Koop BF,Robertsen B

Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production.
Genes & development28(23)2597-612(2014 Dec)
Li Y,Esain V,Teng L,Xu J,Kwan W,Frost IM,Yzaguirre AD,Cai X,Cortes M,Maijenburg MW,Tober J,Dzierzak E,Orkin SH,Tan K,North TE,Speck NA

Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.
PLoS pathogens9(9)e1003619(2013)
Palha N,Guivel-Benhassine F,Briolat V,Lutfalla G,Sourisseau M,Ellett F,Wang CH,Lieschke GJ,Herbomel P,Schwartz O,Levraud JP

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crfb2 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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