TMPRSS4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMPRSS4A
Entrez Gene ID	777630
Full Name	transmembrane protease, serine 4a
Synonyms	tmprss4,zgc:152909
General protein information	Preferred Names transmembrane protease, serine 4a Names transmembrane protease, serine 4a transmembrane protease, serine 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077738.1	NP_001071206.1	transmembrane serine protease 4a
XM_005157490.4	XP_005157547.1	transmembrane protease, serine 4a isoform X1
XM_005157490.3	XP_005157547.1	transmembrane protease, serine 4a isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TMPRSS4	NP_001167022.1
Pan troglodytes (chimpanzee)	TMPRSS4	XP_001159793.1
Rattus norvegicus (Norway rat)	Tmprss4	NP_001102468.1
Gallus gallus (chicken)	TMPRSS4	XP_001233789.3
Danio rerio (zebrafish)	tmprss4a	NP_001071206.1
Drosophila melanogaster (fruit fly)	lambdaTry	NP_610673.1
	TMPRSS4	XP_001092969.2
Canis lupus familiaris (dog)	TMPRSS4	XP_005619775.1
Bos taurus (cattle)	TMPRSS4	NP_001095443.1
Mus musculus (house mouse)	Tmprss4	NP_663378.1
Danio rerio (zebrafish)	tmprss4b	NP_001119849.1
Xenopus tropicalis (tropical clawed frog)	tmprss4	NP_001039074.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Transcriptional and metabolic effects of glucocorticoid receptor ? and ? signaling in zebrafish.
Chatzopoulou A, Roy U, Meijer AH, Alia A, Spaink HP, Schaaf MJ
Endocrinology156(5)1757-69(2015 May)

Pannexin 1 ohnologs in the teleost lineage.
Bond SR, Wang N, Leybaert L, Naus CC
The Journal of membrane biology245(8)483-93(2012 Aug)

Identification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (Danio rerio) and the goldfish (Carassius auratus L.).
Grayfer L, Belosevic M
Developmental and comparative immunology36(2)408-17(2012 Feb)

Morpholino knockdown of the ubiquitously expressed transmembrane serine protease TMPRSS4a in zebrafish embryos exhibits severe defects in organogenesis and cell adhesion.
Ohler A, Becker-Pauly C
Biological chemistry392(7)653-64(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in vivo TMPRSS4a gene silencing caused severe defects in tissue development and cell differentiation including a disturbed skeletal muscle formation, a decelerated heartbeat, and a degenerated vascular system
Title: Morpholino knockdown of the ubiquitously expressed transmembrane serine protease TMPRSS4a in zebrafish embryos exhibits severe defects in organogenesis and cell adhesion.

The following TMPRSS4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMPRSS4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36148	NM_001077738.1 Latest version!	Danio rerio transmembrane serine protease 4a (tmprss4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa20264	XM_005157490.4 Latest version!	Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa20264	XM_005157490.3	Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMPRSS4A ( NM_001077738.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001071206.1 Danio rerio transmembrane serine protease 4a (tmprss4a), mRNA.

Price & Availability↑

CloneID	ODa36148
Clone ID Related Accession (Same CDS sequence)	NM_001077738.1
Accession Version	NM_001077738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-25
Organism	Danio rerio(zebrafish)
Product	transmembrane serine protease 4a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125834.1. ##Evidence-Data-START## Transcript exon combination :: BC125834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

ATGACTGCCC CAAAGACTCA GAAGCCTAAA GGGCCGAACA AGAAGAAGAT CATCATTACA 
GTCCTGATGG TGGTGGTCGT TCTGGCAATT TTGGCTGTGG CCGCTTTCCT CATCAAGCAG 
TTGATCGACA GCAAGTATTT CTTCTGCACA AAATCATGGA AGTTCATCCC GCTGGAGAAG 
GTGTGCGATG GGAAAAATGA CTGTAGTGAA GCCGAAGATG AGTCTGCCTG TGTCACCATG 
TTTAAACCCA ACACCACCTT CCCCTTACGG CTGTACTCTG CCAATAATGT ACTTCAGGTG 
TTGAGTCCTA GTGATAACAC ATGGAAAAGT GTGTGTTCAG AGAGTTTCAC CCAGCAACAT 
GCTGAGACCG CCTGTCAGCT GCTGGGATAT TCAGTTTCTC CAGTTTTCAG CAGCATCGCA 
GTGGGTCCTT TGCCGTCTGA TCTGAAGATA TCTTTCTGCA TGGTGGGCAC AACCAAACCA 
CAGACTTTCC AATCCGCTGT GTCAGATCGT AAGGTATGCA GTACAGGAAC TGTCATCTCA 
TTATCATGCT CTGCAGACTG TGGTTTGTCC AGAAACCAGG ACCGGATTGT TGGTGGAAAG 
GATGCTGACA TTGCGAACTG GCCCTGGCAG GTCAGCTTGC AGTATAGCGG CCAGCATACA 
TGCGGAGGAT CACTGGTGAC TCCAAACTGG GTGGTCACTG CGGCCCACTG TTTCAATGGG 
GATGGCAGGA AAGCTCTGAG CCGGTGGACC GTCGTCTCAG GAATTACGTA TCTGAGTTCT 
ACTCCATCTT CATATGTGAA AGAAATCATC GTTAACAGCA ATTACAAACC AGCAGAAAGT 
GACTTTGACA TTACTATGAT CAAACTTCAG TCTCCAATCA CTGTGTCAGA ATCACGTAGA 
CCAGTGTGTT TACCTCCTCA AAACCTCGGT CTGAAAGGAG GAGACGGTTT AGTTGTGACC 
GGCTGGGGTC ATATGGCAGA AAAAGGAGGA AGCTTGTCTT CAATGTTGCA GAAGGCACAG 
ATTCAGGTTA TAGACAGTGC CCAGTGCTCC AGTCCAACAG TTTACGGCTC ATCCATCACT 
CCCAGGATGA TCTGCGCTGG TGTTATGGCT GGAGGAGTGG ATGCTTGCCA GGGTGATAGC 
GGTGGTCCAC TGGTGCATTT GGCTGACCGC TGGGTGCTGG TTGGAGTAGT CAGCTGGGGT 
GTAGGTTGTG CCAGACCAGG TTTTCCTGGT GTTTACACCA ATGTGGACCA GATGCTTGAC 
TGGGCCCATT CAGTAATGCA GACATATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071206.1
CDS	114..1406
Translation	MTAPKTQKPKGPNKKKIIITVLMVVVVLAILAVAAFLIKQLIDSKYFFCTKSWKFIPLEKVCDGKNDCSEAEDESACVTMFKPNTTFPLRLYSANNVLQVLSPSDNTWKSVCSESFTQQHAETACQLLGYSVSPVFSSIAVGPLPSDLKISFCMVGTTKPQTFQSAVSDRKVCSTGTVISLSCSADCGLSRNQDRIVGGKDADIANWPWQVSLQYSGQHTCGGSLVTPNWVVTAAHCFNGDGRKALSRWTVVSGITYLSSTPSSYVKEIIVNSNYKPAESDFDITMIKLQSPITVSESRRPVCLPPQNLGLKGGDGLVVTGWGHMAEKGGSLSSMLQKAQIQVIDSAQCSSPTVYGSSITPRMICAGVMAGGVDACQGDSGGPLVHLADRWVLVGVVSWGVGCARPGFPGVYTNVDQMLDWAHSVMQTYK

Target ORF information:

RefSeq Version	NM_001077738.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane serine protease 4a (tmprss4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077738.1

ORF Insert Sequence:

ATGACTGCCC CAAAGACTCA GAAGCCTAAA GGGCCGAACA AGAAGAAGAT CATCATTACA 
GTCCTGATGG TGGTGGTCGT TCTGGCAATT TTGGCTGTGG CCGCTTTCCT CATCAAGCAG 
TTGATCGACA GCAAGTATTT CTTCTGCACA AAATCATGGA AGTTCATCCC GCTGGAGAAG 
GTGTGCGATG GGAAAAATGA CTGTAGTGAA GCCGAAGATG AGTCTGCCTG TGTCACCATG 
TTTAAACCCA ACACCACCTT CCCCTTACGG CTGTACTCTG CCAATAATGT ACTTCAGGTG 
TTGAGTCCTA GTGATAACAC ATGGAAAAGT GTGTGTTCAG AGAGTTTCAC CCAGCAACAT 
GCTGAGACCG CCTGTCAGCT GCTGGGATAT TCAGTTTCTC CAGTTTTCAG CAGCATCGCA 
GTGGGTCCTT TGCCGTCTGA TCTGAAGATA TCTTTCTGCA TGGTGGGCAC AACCAAACCA 
CAGACTTTCC AATCCGCTGT GTCAGATCGT AAGGTATGCA GTACAGGAAC TGTCATCTCA 
TTATCATGCT CTGCAGACTG TGGTTTGTCC AGAAACCAGG ACCGGATTGT TGGTGGAAAG 
GATGCTGACA TTGCGAACTG GCCCTGGCAG GTCAGCTTGC AGTATAGCGG CCAGCATACA 
TGCGGAGGAT CACTGGTGAC TCCAAACTGG GTGGTCACTG CGGCCCACTG TTTCAATGGG 
GATGGCAGGA AAGCTCTGAG CCGGTGGACC GTCGTCTCAG GAATTACGTA TCTGAGTTCT 
ACTCCATCTT CATATGTGAA AGAAATCATC GTTAACAGCA ATTACAAACC AGCAGAAAGT 
GACTTTGACA TTACTATGAT CAAACTTCAG TCTCCAATCA CTGTGTCAGA ATCACGTAGA 
CCAGTGTGTT TACCTCCTCA AAACCTCGGT CTGAAAGGAG GAGACGGTTT AGTTGTGACC 
GGCTGGGGTC ATATGGCAGA AAAAGGAGGA AGCTTGTCTT CAATGTTGCA GAAGGCACAG 
ATTCAGGTTA TAGACAGTGC CCAGTGCTCC AGTCCAACAG TTTACGGCTC ATCCATCACT 
CCCAGGATGA TCTGCGCTGG TGTTATGGCT GGAGGAGTGG ATGCTTGCCA GGGTGATAGC 
GGTGGTCCAC TGGTGCATTT GGCTGACCGC TGGGTGCTGG TTGGAGTAGT CAGCTGGGGT 
GTAGGTTGTG CCAGACCAGG TTTTCCTGGT GTTTACACCA ATGTGGACCA GATGCTTGAC 
TGGGCCCATT CAGTAATGCA GACATATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMPRSS4A ( XM_005157490.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157547.1 Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20264
Clone ID Related Accession (Same CDS sequence)	XM_005157490.4 , XM_005157490.3
Accession Version	XM_005157490.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane protease, serine 4a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157490.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTA AAACCCAGGT TCCTGAGGAA AACACGACAC CCTTAAACCC ACCAAAGCCA 
GAGCCTGCAA AACGCACAAA ACTCATGACT GCCCCAAAGA CTCAGAAGCC TAAAGGGCCG 
AACAAGAAGA AGATCATCAT TACAGTCCTG ATGGTGGTGG TCGTTCTGGC AATTTTGGCT 
GTGGCCGCTT TCCTCATCAA GCAGTTGATC GACAGCAAGT ATTTCTTCTG CACAAAATCA 
TGGAAGTTCA TCCCGCTGGA GAAGGTGTGC GATGGGAAAA ATGACTGTAG TGAAGCCGAA 
GATGAGTCTG CCTGTGTCAC CATGTTTAAA CCCAACACCA CCTTCCCCTT ACGGCTGTAC 
TCTGCCAATA ATGTGCTTCA GGTGTTGAGT CCTAGTGATA ACACATGGAA AAGTGTGTGT 
TCAGAGAGTT TCACCCAGCA ACATGCTGAG ACCGCCTGTC AGCTGCTGGG ATATTCAGTT 
TCTCCAGTTT TCAGCAGCAT CGCAGTGGGT CCTTTGCCGT CTGATCTGAA GATATCTTTC 
TGCATGGTGG GCACAACCAA ACCACAGACT TTCCAATCCG CTGTGTCAGA TCGTAAGGTA 
TGCAGTACAG GAACTGTCAT CTCATTATCA TGCTCTGCAG ACTGTGGTTT GTCCAGAAAC 
CAGGACCGGA TTGTTGGTGG AAAGGATGCT GACATTGCGA ACTGGCCCTG GCAGGTCAGC 
TTGCAGTATA GCGGCCAGCA TACATGCGGA GGATCACTGG TGACTCCAAA CTGGGTGGTC 
ACTGCGGCCC ACTGTTTCAA TGGGGATGGC AGGAAAGCTC TGAGCCGGTG GACCGTCGTC 
TCAGGAATTA CGTATCTGAG TTCTACTCCA TCTTCATATG TGAAAGAAAT CATCGTTAAC 
AGCAATTACA AACCAGCAGA AAGTGACTTT GACATTACTA TGATCAAACT TCAGTCTCCA 
ATCACTTTGT CAGAATCACG TAGACCAGTG TGTTTACCTC CTCAAAACCT CGGTCTGAAA 
GGAGGAGACG GTTTAGTTGT CACCGGCTGG GGTCATATGG CAGAAAAAGG AGGAAGCTTG 
TCTTCAATGT TGCAGAAGGC TCAGATTCAG GTTATAGACA GTGCCCAGTG CTCCAGTCCA 
ACAGTGTACG GCTCATCCAT CACTCCCAGG ATGATCTGCG CTGGTGTTAT GGCTGGAGGA 
GTGGATGCTT GCCAGGGTGA TAGCGGTGGT CCACTGGTGC ATTTGGCTGA CCGCTGGGTG 
CTGGTTGGAG TAGTCAGCTG GGGTGTAGGT TGTGCCAGAC CAGGTTTTCC TGGTGTTTAC 
ACCAATGTGG ACCAGATGCT TGACTGGGCC CATTCAGTAA TGCAGACATA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157547.1
CDS	313..1689
Translation	MRTKTQVPEENTTPLNPPKPEPAKRTKLMTAPKTQKPKGPNKKKIIITVLMVVVVLAILAVAAFLIKQLIDSKYFFCTKSWKFIPLEKVCDGKNDCSEAEDESACVTMFKPNTTFPLRLYSANNVLQVLSPSDNTWKSVCSESFTQQHAETACQLLGYSVSPVFSSIAVGPLPSDLKISFCMVGTTKPQTFQSAVSDRKVCSTGTVISLSCSADCGLSRNQDRIVGGKDADIANWPWQVSLQYSGQHTCGGSLVTPNWVVTAAHCFNGDGRKALSRWTVVSGITYLSSTPSSYVKEIIVNSNYKPAESDFDITMIKLQSPITLSESRRPVCLPPQNLGLKGGDGLVVTGWGHMAEKGGSLSSMLQKAQIQVIDSAQCSSPTVYGSSITPRMICAGVMAGGVDACQGDSGGPLVHLADRWVLVGVVSWGVGCARPGFPGVYTNVDQMLDWAHSVMQTYK

Target ORF information:

RefSeq Version	XM_005157490.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157490.4

ORF Insert Sequence:

ATGAGGACTA AAACCCAGGT TCCTGAGGAA AACACGACAC CCTTAAACCC ACCAAAGCCA 
GAGCCTGCAA AACGCACAAA ACTCATGACT GCCCCAAAGA CTCAGAAGCC TAAAGGGCCG 
AACAAGAAGA AGATCATCAT TACAGTCCTG ATGGTGGTGG TCGTTCTGGC AATTTTGGCT 
GTGGCCGCTT TCCTCATCAA GCAGTTGATC GACAGCAAGT ATTTCTTCTG CACAAAATCA 
TGGAAGTTCA TCCCGCTGGA GAAGGTGTGC GATGGGAAAA ATGACTGTAG TGAAGCCGAA 
GATGAGTCTG CCTGTGTCAC CATGTTTAAA CCCAACACCA CCTTCCCCTT ACGGCTGTAC 
TCTGCCAATA ATGTGCTTCA GGTGTTGAGT CCTAGTGATA ACACATGGAA AAGTGTGTGT 
TCAGAGAGTT TCACCCAGCA ACATGCTGAG ACCGCCTGTC AGCTGCTGGG ATATTCAGTT 
TCTCCAGTTT TCAGCAGCAT CGCAGTGGGT CCTTTGCCGT CTGATCTGAA GATATCTTTC 
TGCATGGTGG GCACAACCAA ACCACAGACT TTCCAATCCG CTGTGTCAGA TCGTAAGGTA 
TGCAGTACAG GAACTGTCAT CTCATTATCA TGCTCTGCAG ACTGTGGTTT GTCCAGAAAC 
CAGGACCGGA TTGTTGGTGG AAAGGATGCT GACATTGCGA ACTGGCCCTG GCAGGTCAGC 
TTGCAGTATA GCGGCCAGCA TACATGCGGA GGATCACTGG TGACTCCAAA CTGGGTGGTC 
ACTGCGGCCC ACTGTTTCAA TGGGGATGGC AGGAAAGCTC TGAGCCGGTG GACCGTCGTC 
TCAGGAATTA CGTATCTGAG TTCTACTCCA TCTTCATATG TGAAAGAAAT CATCGTTAAC 
AGCAATTACA AACCAGCAGA AAGTGACTTT GACATTACTA TGATCAAACT TCAGTCTCCA 
ATCACTTTGT CAGAATCACG TAGACCAGTG TGTTTACCTC CTCAAAACCT CGGTCTGAAA 
GGAGGAGACG GTTTAGTTGT CACCGGCTGG GGTCATATGG CAGAAAAAGG AGGAAGCTTG 
TCTTCAATGT TGCAGAAGGC TCAGATTCAG GTTATAGACA GTGCCCAGTG CTCCAGTCCA 
ACAGTGTACG GCTCATCCAT CACTCCCAGG ATGATCTGCG CTGGTGTTAT GGCTGGAGGA 
GTGGATGCTT GCCAGGGTGA TAGCGGTGGT CCACTGGTGC ATTTGGCTGA CCGCTGGGTG 
CTGGTTGGAG TAGTCAGCTG GGGTGTAGGT TGTGCCAGAC CAGGTTTTCC TGGTGTTTAC 
ACCAATGTGG ACCAGATGCT TGACTGGGCC CATTCAGTAA TGCAGACATA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMPRSS4A ( XM_005157490.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005157547.1 Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20264
Clone ID Related Accession (Same CDS sequence)	XM_005157490.4 , XM_005157490.3
Accession Version	XM_005157490.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transmembrane protease, serine 4a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157490.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGACTA AAACCCAGGT TCCTGAGGAA AACACGACAC CCTTAAACCC ACCAAAGCCA 
GAGCCTGCAA AACGCACAAA ACTCATGACT GCCCCAAAGA CTCAGAAGCC TAAAGGGCCG 
AACAAGAAGA AGATCATCAT TACAGTCCTG ATGGTGGTGG TCGTTCTGGC AATTTTGGCT 
GTGGCCGCTT TCCTCATCAA GCAGTTGATC GACAGCAAGT ATTTCTTCTG CACAAAATCA 
TGGAAGTTCA TCCCGCTGGA GAAGGTGTGC GATGGGAAAA ATGACTGTAG TGAAGCCGAA 
GATGAGTCTG CCTGTGTCAC CATGTTTAAA CCCAACACCA CCTTCCCCTT ACGGCTGTAC 
TCTGCCAATA ATGTGCTTCA GGTGTTGAGT CCTAGTGATA ACACATGGAA AAGTGTGTGT 
TCAGAGAGTT TCACCCAGCA ACATGCTGAG ACCGCCTGTC AGCTGCTGGG ATATTCAGTT 
TCTCCAGTTT TCAGCAGCAT CGCAGTGGGT CCTTTGCCGT CTGATCTGAA GATATCTTTC 
TGCATGGTGG GCACAACCAA ACCACAGACT TTCCAATCCG CTGTGTCAGA TCGTAAGGTA 
TGCAGTACAG GAACTGTCAT CTCATTATCA TGCTCTGCAG ACTGTGGTTT GTCCAGAAAC 
CAGGACCGGA TTGTTGGTGG AAAGGATGCT GACATTGCGA ACTGGCCCTG GCAGGTCAGC 
TTGCAGTATA GCGGCCAGCA TACATGCGGA GGATCACTGG TGACTCCAAA CTGGGTGGTC 
ACTGCGGCCC ACTGTTTCAA TGGGGATGGC AGGAAAGCTC TGAGCCGGTG GACCGTCGTC 
TCAGGAATTA CGTATCTGAG TTCTACTCCA TCTTCATATG TGAAAGAAAT CATCGTTAAC 
AGCAATTACA AACCAGCAGA AAGTGACTTT GACATTACTA TGATCAAACT TCAGTCTCCA 
ATCACTTTGT CAGAATCACG TAGACCAGTG TGTTTACCTC CTCAAAACCT CGGTCTGAAA 
GGAGGAGACG GTTTAGTTGT CACCGGCTGG GGTCATATGG CAGAAAAAGG AGGAAGCTTG 
TCTTCAATGT TGCAGAAGGC TCAGATTCAG GTTATAGACA GTGCCCAGTG CTCCAGTCCA 
ACAGTGTACG GCTCATCCAT CACTCCCAGG ATGATCTGCG CTGGTGTTAT GGCTGGAGGA 
GTGGATGCTT GCCAGGGTGA TAGCGGTGGT CCACTGGTGC ATTTGGCTGA CCGCTGGGTG 
CTGGTTGGAG TAGTCAGCTG GGGTGTAGGT TGTGCCAGAC CAGGTTTTCC TGGTGTTTAC 
ACCAATGTGG ACCAGATGCT TGACTGGGCC CATTCAGTAA TGCAGACATA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157547.1
CDS	343..1719
Translation	MRTKTQVPEENTTPLNPPKPEPAKRTKLMTAPKTQKPKGPNKKKIIITVLMVVVVLAILAVAAFLIKQLIDSKYFFCTKSWKFIPLEKVCDGKNDCSEAEDESACVTMFKPNTTFPLRLYSANNVLQVLSPSDNTWKSVCSESFTQQHAETACQLLGYSVSPVFSSIAVGPLPSDLKISFCMVGTTKPQTFQSAVSDRKVCSTGTVISLSCSADCGLSRNQDRIVGGKDADIANWPWQVSLQYSGQHTCGGSLVTPNWVVTAAHCFNGDGRKALSRWTVVSGITYLSSTPSSYVKEIIVNSNYKPAESDFDITMIKLQSPITLSESRRPVCLPPQNLGLKGGDGLVVTGWGHMAEKGGSLSSMLQKAQIQVIDSAQCSSPTVYGSSITPRMICAGVMAGGVDACQGDSGGPLVHLADRWVLVGVVSWGVGCARPGFPGVYTNVDQMLDWAHSVMQTYK

Target ORF information:

RefSeq Version	XM_005157490.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protease, serine 4a (tmprss4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157490.3

ORF Insert Sequence:

ATGAGGACTA AAACCCAGGT TCCTGAGGAA AACACGACAC CCTTAAACCC ACCAAAGCCA 
GAGCCTGCAA AACGCACAAA ACTCATGACT GCCCCAAAGA CTCAGAAGCC TAAAGGGCCG 
AACAAGAAGA AGATCATCAT TACAGTCCTG ATGGTGGTGG TCGTTCTGGC AATTTTGGCT 
GTGGCCGCTT TCCTCATCAA GCAGTTGATC GACAGCAAGT ATTTCTTCTG CACAAAATCA 
TGGAAGTTCA TCCCGCTGGA GAAGGTGTGC GATGGGAAAA ATGACTGTAG TGAAGCCGAA 
GATGAGTCTG CCTGTGTCAC CATGTTTAAA CCCAACACCA CCTTCCCCTT ACGGCTGTAC 
TCTGCCAATA ATGTGCTTCA GGTGTTGAGT CCTAGTGATA ACACATGGAA AAGTGTGTGT 
TCAGAGAGTT TCACCCAGCA ACATGCTGAG ACCGCCTGTC AGCTGCTGGG ATATTCAGTT 
TCTCCAGTTT TCAGCAGCAT CGCAGTGGGT CCTTTGCCGT CTGATCTGAA GATATCTTTC 
TGCATGGTGG GCACAACCAA ACCACAGACT TTCCAATCCG CTGTGTCAGA TCGTAAGGTA 
TGCAGTACAG GAACTGTCAT CTCATTATCA TGCTCTGCAG ACTGTGGTTT GTCCAGAAAC 
CAGGACCGGA TTGTTGGTGG AAAGGATGCT GACATTGCGA ACTGGCCCTG GCAGGTCAGC 
TTGCAGTATA GCGGCCAGCA TACATGCGGA GGATCACTGG TGACTCCAAA CTGGGTGGTC 
ACTGCGGCCC ACTGTTTCAA TGGGGATGGC AGGAAAGCTC TGAGCCGGTG GACCGTCGTC 
TCAGGAATTA CGTATCTGAG TTCTACTCCA TCTTCATATG TGAAAGAAAT CATCGTTAAC 
AGCAATTACA AACCAGCAGA AAGTGACTTT GACATTACTA TGATCAAACT TCAGTCTCCA 
ATCACTTTGT CAGAATCACG TAGACCAGTG TGTTTACCTC CTCAAAACCT CGGTCTGAAA 
GGAGGAGACG GTTTAGTTGT CACCGGCTGG GGTCATATGG CAGAAAAAGG AGGAAGCTTG 
TCTTCAATGT TGCAGAAGGC TCAGATTCAG GTTATAGACA GTGCCCAGTG CTCCAGTCCA 
ACAGTGTACG GCTCATCCAT CACTCCCAGG ATGATCTGCG CTGGTGTTAT GGCTGGAGGA 
GTGGATGCTT GCCAGGGTGA TAGCGGTGGT CCACTGGTGC ATTTGGCTGA CCGCTGGGTG 
CTGGTTGGAG TAGTCAGCTG GGGTGTAGGT TGTGCCAGAC CAGGTTTTCC TGGTGTTTAC 
ACCAATGTGG ACCAGATGCT TGACTGGGCC CATTCAGTAA TGCAGACATA TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Transcriptional and metabolic effects of glucocorticoid receptor ? and ? signaling in zebrafish.
Endocrinology156(5)1757-69(2015 May)
Chatzopoulou A,Roy U,Meijer AH,Alia A,Spaink HP,Schaaf MJ

Pannexin 1 ohnologs in the teleost lineage.
The Journal of membrane biology245(8)483-93(2012 Aug)
Bond SR,Wang N,Leybaert L,Naus CC

Identification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (Danio rerio) and the goldfish (Carassius auratus L.).
Developmental and comparative immunology36(2)408-17(2012 Feb)
Grayfer L,Belosevic M

Morpholino knockdown of the ubiquitously expressed transmembrane serine protease TMPRSS4a in zebrafish embryos exhibits severe defects in organogenesis and cell adhesion.
Biological chemistry392(7)653-64(2011 Jul)
Ohler A,Becker-Pauly C