afap1l1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	afap1l1b
Entrez Gene ID	768144
Full Name	actin filament associated protein 1-like 1b
Synonyms	sb:cb932,zgc:153096
General protein information	Preferred Names actin filament associated protein 1-like 1b Names actin filament-associated protein 1-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468916.1	XP_021324591.1	actin filament-associated protein 1-like 1 isoform X2
XM_005161304.3	XP_005161361.1	actin filament-associated protein 1-like 1 isoform X1
NM_001077287.2	NP_001070755.2	actin filament-associated protein 1-like 1
XM_005161304.4	XP_005161361.1	actin filament-associated protein 1-like 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100535038	XP_005173382.1
Danio rerio (zebrafish)	afap1l1b	NP_001070755.2
Danio rerio (zebrafish)	afap1l1a	NP_001025384.1
Danio rerio (zebrafish)	LOC100535279	XP_005173387.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution and expression of the phosphodiesterase 6 genes unveils vertebrate novelty to control photosensitivity.
Lagman D, Franz?n IE, Eggert J, Larhammar D, Abalo XM
BMC evolutionary biology16(1)124(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Global analysis of the haematopoietic and endothelial transcriptome during zebrafish development.
Cannon JE, Place ES, Eve AM, Bradshaw CR, Sesay A, Morrell NW, Smith JC
Mechanisms of development130(2-3)122-31(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following afap1l1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afap1l1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36073	NM_001077287.2 Latest version!	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa26039	XM_005161304.4 Latest version!	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa46560	XM_021468916.1 Latest version!	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa26039	XM_005161304.3	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

afap1l1b ( NM_001077287.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070755.2 Danio rerio actin filament associated protein 1-like 1b (afap1l1b), mRNA.

Price & Availability↑

CloneID	ODa36073
Clone ID Related Accession (Same CDS sequence)	NM_001077287.2
Accession Version	NM_001077287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1-like 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR749164.18. On Nov 25, 2009 this sequence version replaced NM_001077287.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA4476756 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGGAGAATGA GTCAAAACTG 
AAGCGTTATT CCTCCATGTC CAGTGTGGAT ACAGCCAAGG GTGACACTCA GATCACAGTG 
AAGCGACACG GCTCCTCTGT GAATCACTAT GGTAAATATG GAAAAACGAG GGCGGAAGAA 
GACGCACGAC GGTTTCTAAA TGAGAAAGAG CAGCTGGAGA AAGAGAAAGA AGAAATCAAG 
GAGAGCCTGG TGTCACTGCG CTCCGAACGG AGTGAAGTGA GAAAAGGGAT CAAAAATGCA 
ACAGATCTAA AAAAGAAAGC CATGGAGCAG CGTCTGGCCC AGCTGGAGGA AAGCTGTCGT 
CAGAAAGAAG CTGAGCGAGT CGACCTGGAG CTGAGACTCA CAGAAGTGAA GGAGAAACTG 
AAGAAATCTC TGACCGGAGG AATGCTGGGG GCGCCTCAGG AAAAGAAACC CACGATTAAG 
GCTTCAGTTA AGAAGAAGGA ACATCTTTAC ACTGATGCCA CTGTGCCGGT CAACTGTGCA 
TCTGAAATGA AGAAACGCCC ACTTTCCATC TATGCATCCG CTAAAGGAAA TGTCATGCAG 
AAAGCTAAGG AATGGGAGTC AAAGAAAGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070755.2
CDS	87..2342
Translation	MEDSTCIEHLVRELSLLLKLLENESVSSATAEKMNVVRNLVKQLQPSVNGSDFIYMNTSLYGNGTSFVESLFEEFDCDLQDLRDSPEEMKDVEQEEASKHMPIKSSPTDTPPPPLPTTPPPDDYYEEAAPLGPGTAPQYITTRNNSSPPNSIEDAYYEDADNNYPTTRLNGASKNSYNDSDALSSSYESYDEEDEEAKGRERTHQWPSEESPLGQMKDCRKCAFLLRKKRFGQWAKQLTIIRENKLQCFKNPKDRSPHVDLPLNLCNVIYVPKDGRRKKHELRFSLPGGEALVLAVQSKEQAESWLKVIQEVSNLANNMNGKDGSSSPMILRKLELDKMMSSDKHTSDSDSAANGDVTRDICENGKAKKGTFSELTGSMSRAAGRKINRIISFSKKKPSLPGETNMLFHLDDNPRCGYLNVLVNQCWKERWCCVRNRTLYFHKDRGDVHTHVRALALHGVDVLPGLGPKHPFAFRIMRASTEVAALEASCSVEMGRWLGVLLAEAGSATVPEELHYDYVDVDTISSIQNAARNSFLWATSDPRMYDEVPYESILENESKLKRYSSMSSVDTAKGDTQITVKRHGSSVNHYGKYGKTRAEEDARRFLNEKEQLEKEKEEIKESLVSLRSERSEVRKGIKNATDLKKKAMEQRLAQLEESCRQKEAERVDLELRLTEVKEKLKKSLTGGMLGAPQEKKPTIKASVKKKEHLYTDATVPVNCASEMKKRPLSIYASAKGNVMQKAKEWESKKVK

Target ORF information:

RefSeq Version	NM_001077287.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077287.2

ORF Insert Sequence:

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGGAGAATGA GTCAAAACTG 
AAGCGTTATT CCTCCATGTC CAGTGTGGAT ACAGCCAAGG GTGACACTCA GATCACAGTG 
AAGCGACACG GCTCCTCTGT GAATCACTAT GGTAAATATG GAAAAACGAG GGCGGAAGAA 
GACGCACGAC GGTTTCTAAA TGAGAAAGAG CAGCTGGAGA AAGAGAAAGA AGAAATCAAG 
GAGAGCCTGG TGTCACTGCG CTCCGAACGG AGTGAAGTGA GAAAAGGGAT CAAAAATGCA 
ACAGATCTAA AAAAGAAAGC CATGGAGCAG CGTCTGGCCC AGCTGGAGGA AAGCTGTCGT 
CAGAAAGAAG CTGAGCGAGT CGACCTGGAG CTGAGACTCA CAGAAGTGAA GGAGAAACTG 
AAGAAATCTC TGACCGGAGG AATGCTGGGG GCGCCTCAGG AAAAGAAACC CACGATTAAG 
GCTTCAGTTA AGAAGAAGGA ACATCTTTAC ACTGATGCCA CTGTGCCGGT CAACTGTGCA 
TCTGAAATGA AGAAACGCCC ACTTTCCATC TATGCATCCG CTAAAGGAAA TGTCATGCAG 
AAAGCTAAGG AATGGGAGTC AAAGAAAGTG AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1l1b ( XM_005161304.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161361.1 Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26039
Clone ID Related Accession (Same CDS sequence)	XM_005161304.3 , XM_005161304.4
Accession Version	XM_005161304.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	actin filament-associated protein 1-like 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGCAGGCGGA GGAGAATGAG 
TCAAAACTGA AGCGTTATTC CTCCATGTCC AGTGTGGATA CAGCCAAGGG TGACACTCAG 
ATCACAGTGA AGCGACACGG CTCCTCTGTG AATCACTATG GTAAATATGG AAAAACGAGG 
GCGGAAGAAG ACGCACGACG GTTTCTAAAT GAGAAAGAGC AGCTGGAGAA AGAGAAAGAA 
GAAATCAAGG AGAGCCTGGT GTCACTGCGC TCCGAACGGA GTGAAGTGAG AAAAGGGATC 
AAAAATGCAA CAGATCTAAA AAAGAAAGCC ATGGAGCAGC GTCTGGCCCA GCTGGAGGAA 
AGCTGTCGTC AGAAAGAAGC TGAGCGAGTC GACCTGGAGC TGAGACTCAC AGAAGTGAAG 
GAGAAACTGA AGAAATCTCT GACCGGAGGA ATGCTGGGGG CGCCTCAGGA AAAGAAACCC 
ACGATTAAGG CTTCAGTTAA GAAGAAGGAA CATCTTTACA CTGATGCCAC TGTGCCGGTC 
AACTGTGCAT CTGAAATGAA GAAACGCCCA CTTTCCATCT ATGCATCCGC TAAAGGAAAT 
GTCATGCAGA AAGCTAAGGA ATGGGAGTCA AAGAAAGTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161361.1
CDS	94..2358
Translation	MEDSTCIEHLVRELSLLLKLLENESVSSATAEKMNVVRNLVKQLQPSVNGSDFIYMNTSLYGNGTSFVESLFEEFDCDLQDLRDSPEEMKDVEQEEASKHMPIKSSPTDTPPPPLPTTPPPDDYYEEAAPLGPGTAPQYITTRNNSSPPNSIEDAYYEDADNNYPTTRLNGASKNSYNDSDALSSSYESYDEEDEEAKGRERTHQWPSEESPLGQMKDCRKCAFLLRKKRFGQWAKQLTIIRENKLQCFKNPKDRSPHVDLPLNLCNVIYVPKDGRRKKHELRFSLPGGEALVLAVQSKEQAESWLKVIQEVSNLANNMNGKDGSSSPMILRKLELDKMMSSDKHTSDSDSAANGDVTRDICENGKAKKGTFSELTGSMSRAAGRKINRIISFSKKKPSLPGETNMLFHLDDNPRCGYLNVLVNQCWKERWCCVRNRTLYFHKDRGDVHTHVRALALHGVDVLPGLGPKHPFAFRIMRASTEVAALEASCSVEMGRWLGVLLAEAGSATVPEELHYDYVDVDTISSIQNAARNSFLWATSDPRMYDEVPYESILQAEENESKLKRYSSMSSVDTAKGDTQITVKRHGSSVNHYGKYGKTRAEEDARRFLNEKEQLEKEKEEIKESLVSLRSERSEVRKGIKNATDLKKKAMEQRLAQLEESCRQKEAERVDLELRLTEVKEKLKKSLTGGMLGAPQEKKPTIKASVKKKEHLYTDATVPVNCASEMKKRPLSIYASAKGNVMQKAKEWESKKVK

Target ORF information:

RefSeq Version	XM_005161304.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161304.3

ORF Insert Sequence:

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGCAGGCGGA GGAGAATGAG 
TCAAAACTGA AGCGTTATTC CTCCATGTCC AGTGTGGATA CAGCCAAGGG TGACACTCAG 
ATCACAGTGA AGCGACACGG CTCCTCTGTG AATCACTATG GTAAATATGG AAAAACGAGG 
GCGGAAGAAG ACGCACGACG GTTTCTAAAT GAGAAAGAGC AGCTGGAGAA AGAGAAAGAA 
GAAATCAAGG AGAGCCTGGT GTCACTGCGC TCCGAACGGA GTGAAGTGAG AAAAGGGATC 
AAAAATGCAA CAGATCTAAA AAAGAAAGCC ATGGAGCAGC GTCTGGCCCA GCTGGAGGAA 
AGCTGTCGTC AGAAAGAAGC TGAGCGAGTC GACCTGGAGC TGAGACTCAC AGAAGTGAAG 
GAGAAACTGA AGAAATCTCT GACCGGAGGA ATGCTGGGGG CGCCTCAGGA AAAGAAACCC 
ACGATTAAGG CTTCAGTTAA GAAGAAGGAA CATCTTTACA CTGATGCCAC TGTGCCGGTC 
AACTGTGCAT CTGAAATGAA GAAACGCCCA CTTTCCATCT ATGCATCCGC TAAAGGAAAT 
GTCATGCAGA AAGCTAAGGA ATGGGAGTCA AAGAAAGTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1l1b ( XM_005161304.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161361.1 Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26039
Clone ID Related Accession (Same CDS sequence)	XM_005161304.3 , XM_005161304.4
Accession Version	XM_005161304.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1-like 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161304.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGCAGGCGGA GGAGAATGAG 
TCAAAACTGA AGCGTTATTC CTCCATGTCC AGTGTGGATA CAGCCAAGGG TGACACTCAG 
ATCACAGTGA AGCGACACGG CTCCTCTGTG AATCACTATG GTAAATATGG AAAAACGAGG 
GCGGAAGAAG ACGCACGACG GTTTCTAAAT GAGAAAGAGC AGCTGGAGAA AGAGAAAGAA 
GAAATCAAGG AGAGCCTGGT GTCACTGCGC TCCGAACGGA GTGAAGTGAG AAAAGGGATC 
AAAAATGCAA CAGATCTAAA AAAGAAAGCC ATGGAGCAGC GTCTGGCCCA GCTGGAGGAA 
AGCTGTCGTC AGAAAGAAGC TGAGCGAGTC GACCTGGAGC TGAGACTCAC AGAAGTGAAG 
GAGAAACTGA AGAAATCTCT GACCGGAGGA ATGCTGGGGG CGCCTCAGGA AAAGAAACCC 
ACGATTAAGG CTTCAGTTAA GAAGAAGGAA CATCTTTACA CTGATGCCAC TGTGCCGGTC 
AACTGTGCAT CTGAAATGAA GAAACGCCCA CTTTCCATCT ATGCATCCGC TAAAGGAAAT 
GTCATGCAGA AAGCTAAGGA ATGGGAGTCA AAGAAAGTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161361.1
CDS	100..2364
Translation	MEDSTCIEHLVRELSLLLKLLENESVSSATAEKMNVVRNLVKQLQPSVNGSDFIYMNTSLYGNGTSFVESLFEEFDCDLQDLRDSPEEMKDVEQEEASKHMPIKSSPTDTPPPPLPTTPPPDDYYEEAAPLGPGTAPQYITTRNNSSPPNSIEDAYYEDADNNYPTTRLNGASKNSYNDSDALSSSYESYDEEDEEAKGRERTHQWPSEESPLGQMKDCRKCAFLLRKKRFGQWAKQLTIIRENKLQCFKNPKDRSPHVDLPLNLCNVIYVPKDGRRKKHELRFSLPGGEALVLAVQSKEQAESWLKVIQEVSNLANNMNGKDGSSSPMILRKLELDKMMSSDKHTSDSDSAANGDVTRDICENGKAKKGTFSELTGSMSRAAGRKINRIISFSKKKPSLPGETNMLFHLDDNPRCGYLNVLVNQCWKERWCCVRNRTLYFHKDRGDVHTHVRALALHGVDVLPGLGPKHPFAFRIMRASTEVAALEASCSVEMGRWLGVLLAEAGSATVPEELHYDYVDVDTISSIQNAARNSFLWATSDPRMYDEVPYESILQAEENESKLKRYSSMSSVDTAKGDTQITVKRHGSSVNHYGKYGKTRAEEDARRFLNEKEQLEKEKEEIKESLVSLRSERSEVRKGIKNATDLKKKAMEQRLAQLEESCRQKEAERVDLELRLTEVKEKLKKSLTGGMLGAPQEKKPTIKASVKKKEHLYTDATVPVNCASEMKKRPLSIYASAKGNVMQKAKEWESKKVK

Target ORF information:

RefSeq Version	XM_005161304.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161304.4

ORF Insert Sequence:

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGCAGGCGGA GGAGAATGAG 
TCAAAACTGA AGCGTTATTC CTCCATGTCC AGTGTGGATA CAGCCAAGGG TGACACTCAG 
ATCACAGTGA AGCGACACGG CTCCTCTGTG AATCACTATG GTAAATATGG AAAAACGAGG 
GCGGAAGAAG ACGCACGACG GTTTCTAAAT GAGAAAGAGC AGCTGGAGAA AGAGAAAGAA 
GAAATCAAGG AGAGCCTGGT GTCACTGCGC TCCGAACGGA GTGAAGTGAG AAAAGGGATC 
AAAAATGCAA CAGATCTAAA AAAGAAAGCC ATGGAGCAGC GTCTGGCCCA GCTGGAGGAA 
AGCTGTCGTC AGAAAGAAGC TGAGCGAGTC GACCTGGAGC TGAGACTCAC AGAAGTGAAG 
GAGAAACTGA AGAAATCTCT GACCGGAGGA ATGCTGGGGG CGCCTCAGGA AAAGAAACCC 
ACGATTAAGG CTTCAGTTAA GAAGAAGGAA CATCTTTACA CTGATGCCAC TGTGCCGGTC 
AACTGTGCAT CTGAAATGAA GAAACGCCCA CTTTCCATCT ATGCATCCGC TAAAGGAAAT 
GTCATGCAGA AAGCTAAGGA ATGGGAGTCA AAGAAAGTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1l1b ( XM_021468916.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324591.1 Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46560
Clone ID Related Accession (Same CDS sequence)	XM_021468916.1
Accession Version	XM_021468916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	actin filament-associated protein 1-like 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGGCGGAGGA GAATGAGTCA 
AAACTGAAGC GTTATTCCTC CATGTCCAGT GTGGATACAG CCAAGGGTGA CACTCAGATC 
ACAGTGAAGC GACACGGCTC CTCTGTGAAT CACTATGGTA AATATGGAAA AACGAGGGCG 
GAAGAAGACG CACGACGGTT TCTAAATGAG AAAGAGCAGC TGGAGAAAGA GAAAGAAGAA 
ATCAAGGAGA GCCTGGTGTC ACTGCGCTCC GAACGGAGTG AAGTGAGAAA AGGGATCAAA 
AATGCAACAG ATCTAAAAAA GAAAGCCATG GAGCAGCGTC TGGCCCAGCT GGAGGAAAGC 
TGTCGTCAGA AAGAAGCTGA GCGAGTCGAC CTGGAGCTGA GACTCACAGA AGTGAAGGAG 
AAACTGAAGA AATCTCTGAC CGGAGGAATG CTGGGGGCGC CTCAGGAAAA GAAACCCACG 
ATTAAGGCTT CAGTTAAGAA GAAGGAACAT CTTTACACTG ATGCCACTGT GCCGGTCAAC 
TGTGCATCTG AAATGAAGAA ACGCCCACTT TCCATCTATG CATCCGCTAA AGGAAATGTC 
ATGCAGAAAG CTAAGGAATG GGAGTCAAAG AAAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324591.1
CDS	100..2361
Translation	MEDSTCIEHLVRELSLLLKLLENESVSSATAEKMNVVRNLVKQLQPSVNGSDFIYMNTSLYGNGTSFVESLFEEFDCDLQDLRDSPEEMKDVEQEEASKHMPIKSSPTDTPPPPLPTTPPPDDYYEEAAPLGPGTAPQYITTRNNSSPPNSIEDAYYEDADNNYPTTRLNGASKNSYNDSDALSSSYESYDEEDEEAKGRERTHQWPSEESPLGQMKDCRKCAFLLRKKRFGQWAKQLTIIRENKLQCFKNPKDRSPHVDLPLNLCNVIYVPKDGRRKKHELRFSLPGGEALVLAVQSKEQAESWLKVIQEVSNLANNMNGKDGSSSPMILRKLELDKMMSSDKHTSDSDSAANGDVTRDICENGKAKKGTFSELTGSMSRAAGRKINRIISFSKKKPSLPGETNMLFHLDDNPRCGYLNVLVNQCWKERWCCVRNRTLYFHKDRGDVHTHVRALALHGVDVLPGLGPKHPFAFRIMRASTEVAALEASCSVEMGRWLGVLLAEAGSATVPEELHYDYVDVDTISSIQNAARNSFLWATSDPRMYDEVPYESILAEENESKLKRYSSMSSVDTAKGDTQITVKRHGSSVNHYGKYGKTRAEEDARRFLNEKEQLEKEKEEIKESLVSLRSERSEVRKGIKNATDLKKKAMEQRLAQLEESCRQKEAERVDLELRLTEVKEKLKKSLTGGMLGAPQEKKPTIKASVKKKEHLYTDATVPVNCASEMKKRPLSIYASAKGNVMQKAKEWESKKVK

Target ORF information:

RefSeq Version	XM_021468916.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actin filament associated protein 1-like 1b (afap1l1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468916.1

ORF Insert Sequence:

ATGGAGGACT CCACCTGTAT AGAGCATCTG GTGAGGGAGC TGAGTTTGCT GCTGAAGCTG 
CTTGAGAACG AAAGTGTGAG TTCAGCCACC GCAGAGAAGA TGAACGTGGT CAGAAACCTG 
GTCAAACAGC TGCAGCCGTC TGTGAATGGA TCTGACTTCA TATACATGAA CACGTCTCTA 
TATGGAAATG GTACCAGCTT TGTTGAATCA CTGTTTGAAG AATTTGACTG TGACCTGCAG 
GATCTCAGGG ACTCGCCTGA AGAAATGAAA GATGTGGAGC AAGAGGAGGC ATCTAAGCAC 
ATGCCTATAA AATCGAGTCC CACAGACACT CCTCCTCCTC CTCTTCCTAC AACTCCTCCA 
CCTGATGACT ACTATGAAGA AGCTGCCCCG CTGGGTCCGG GCACAGCGCC GCAGTATATA 
ACCACACGCA ACAACTCCAG CCCTCCAAAC TCAATAGAGG ATGCTTATTA TGAAGACGCT 
GACAACAATT ACCCAACAAC TCGCCTGAAC GGGGCCAGCA AGAACTCCTA CAATGATTCA 
GATGCTCTCA GCAGTTCTTA TGAGTCATAT GATGAAGAGG ATGAGGAAGC CAAAGGCAGA 
GAGCGGACTC ATCAGTGGCC ATCAGAAGAA AGTCCGCTAG GCCAAATGAA AGACTGCCGC 
AAATGTGCCT TCCTGCTGCG CAAGAAACGA TTCGGTCAGT GGGCGAAACA GCTCACAATC 
ATTCGTGAAA ACAAACTTCA GTGCTTTAAG AATCCTAAAG ATCGCTCTCC TCATGTTGAC 
CTCCCGCTAA ATCTTTGCAA CGTCATCTAT GTGCCCAAAG ATGGACGACG CAAGAAGCAC 
GAACTCCGCT TCTCCCTGCC GGGAGGAGAG GCTCTAGTAC TGGCTGTACA GAGCAAAGAA 
CAGGCCGAGA GCTGGCTAAA GGTCATTCAG GAAGTCAGTA ACCTGGCTAA CAACATGAAT 
GGAAAAGACG GTTCATCCTC TCCAATGATA CTGCGGAAAC TAGAGCTGGA TAAGATGATG 
TCATCAGATA AACATACCTC TGATTCTGAC AGCGCAGCCA ATGGAGATGT TACTCGCGAC 
ATCTGTGAGA ACGGAAAGGC CAAGAAAGGG ACGTTTTCTG AGCTAACTGG ATCTATGAGT 
CGAGCTGCTG GCAGGAAAAT CAACCGCATC ATCAGTTTCT CCAAGAAGAA GCCGTCTTTG 
CCGGGCGAAA CGAACATGCT TTTCCACCTG GATGACAATC CTCGTTGTGG TTACTTGAAC 
GTCCTGGTCA ACCAGTGCTG GAAGGAGCGC TGGTGTTGTG TGAGGAACAG GACTCTGTAT 
TTTCATAAGG ACAGAGGAGA CGTTCACACC CATGTTAGAG CTTTAGCTCT TCATGGTGTA 
GATGTTTTAC CTGGACTTGG ACCCAAACAT CCTTTTGCCT TCCGCATCAT GCGAGCAAGT 
ACAGAAGTGG CTGCACTTGA GGCAAGCTGT TCTGTGGAGA TGGGTCGCTG GTTAGGTGTT 
TTGTTGGCTG AAGCAGGAAG CGCCACCGTC CCAGAGGAGC TGCATTATGA CTATGTGGAT 
GTGGACACCA TCAGTAGCAT ACAGAACGCA GCAAGAAACT CTTTTCTATG GGCAACTTCA 
GACCCACGGA TGTACGATGA AGTTCCCTAT GAAAGTATCC TGGCGGAGGA GAATGAGTCA 
AAACTGAAGC GTTATTCCTC CATGTCCAGT GTGGATACAG CCAAGGGTGA CACTCAGATC 
ACAGTGAAGC GACACGGCTC CTCTGTGAAT CACTATGGTA AATATGGAAA AACGAGGGCG 
GAAGAAGACG CACGACGGTT TCTAAATGAG AAAGAGCAGC TGGAGAAAGA GAAAGAAGAA 
ATCAAGGAGA GCCTGGTGTC ACTGCGCTCC GAACGGAGTG AAGTGAGAAA AGGGATCAAA 
AATGCAACAG ATCTAAAAAA GAAAGCCATG GAGCAGCGTC TGGCCCAGCT GGAGGAAAGC 
TGTCGTCAGA AAGAAGCTGA GCGAGTCGAC CTGGAGCTGA GACTCACAGA AGTGAAGGAG 
AAACTGAAGA AATCTCTGAC CGGAGGAATG CTGGGGGCGC CTCAGGAAAA GAAACCCACG 
ATTAAGGCTT CAGTTAAGAA GAAGGAACAT CTTTACACTG ATGCCACTGT GCCGGTCAAC 
TGTGCATCTG AAATGAAGAA ACGCCCACTT TCCATCTATG CATCCGCTAA AGGAAATGTC 
ATGCAGAAAG CTAAGGAATG GGAGTCAAAG AAAGTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution and expression of the phosphodiesterase 6 genes unveils vertebrate novelty to control photosensitivity.
BMC evolutionary biology16(1)124(2016 Jun)
Lagman D,Franz?n IE,Eggert J,Larhammar D,Abalo XM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Global analysis of the haematopoietic and endothelial transcriptome during zebrafish development.
Mechanisms of development130(2-3)122-31(2013 Feb)
Cannon JE,Place ES,Eve AM,Bradshaw CR,Sesay A,Morrell NW,Smith JC

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afap1l1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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