runx1t1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	runx1t1
Entrez Gene ID	767809
Full Name	runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Synonyms	MTG8,si:ch211-232j17.1,wu:fi14b07,zgc:154044
General protein information	Preferred Names runt-related transcription factor 1; translocated to, 1 (cyclin D-related) Names protein CBFA2T1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017352276.1	XP_017207765.1	protein CBFA2T1 isoform X3
XM_017352275.1	XP_017207764.1	protein CBFA2T1 isoform X2
XM_017352275.2	XP_017207764.1	protein CBFA2T1 isoform X2
XM_017352276.2	XP_017207765.1	protein CBFA2T1 isoform X3
NM_001076776.4	NP_001070244.2	protein CBFA2T1
XM_017352274.1	XP_017207763.1	protein CBFA2T1 isoform X1
XM_017352274.2	XP_017207763.1	protein CBFA2T1 isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	runx1t1	XP_002934461.1
Gallus gallus (chicken)	RUNX1T1	NP_990075.1
Homo sapiens (human)	RUNX1T1	NP_001185608.1
Pan troglodytes (chimpanzee)	RUNX1T1	XP_003311880.1
Canis lupus familiaris (dog)	RUNX1T1	XP_544173.3
Mus musculus (house mouse)	Runx1t1	NP_001104497.1
Danio rerio (zebrafish)	runx1t1	NP_001070244.2
	RUNX1T1	XP_002805480.1
Rattus norvegicus (Norway rat)	Runx1t1	NP_001102127.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following runx1t1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the runx1t1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00144	NM_001076776.4 Latest version!	Danio rerio RUNX1 partner transcriptional co-repressor 1 (runx1t1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa56084	XM_017352274.2 Latest version!	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56085	XM_017352275.2 Latest version!	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56086	XM_017352276.2 Latest version!	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa56085	XM_017352275.1	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56086	XM_017352276.1	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa56084	XM_017352274.1	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

runx1t1 ( NM_001076776.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070244.2 Danio rerio RUNX1 partner transcriptional co-repressor 1 (runx1t1), mRNA.

Price & Availability↑

CloneID	ODa00144
Clone ID Related Accession (Same CDS sequence)	NM_001076776.4
Accession Version	NM_001076776.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Danio rerio(zebrafish)
Product	protein CBFA2T1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR759910.13 and CR735113.6. On Nov 18, 2014 this sequence version replaced NM_001076776.3. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript and protein alignments, including human NP_001185562.1 and mouse NP_001104496.1, and RNASeq data. ##Evidence-Data-START## Transcript exon combination :: GDQH01018112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505377, SAMEA3505380 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATCTCCTGA ACTGCATTAT GGATATGGTG GAGAAGACAC GTCGCTCATT GACAGTGTTG 
CGGAGGTGCC AGGAGACTGA CCGAGAAGAG CTCAACTACT GGATCCGGCG TTACAGCGAT 
GCTGAAGACC TTAAAAAAAC CAGCAGCACT AGCACCAGTC AGTCAAGACA ACAGAGTCCC 
GCTAGCCAAG AAGGCAATGC TTTAGAAGTA CATAGGGAGC TCCTTCACAG GCCAGTGTCT 
GGATACGTAC CTGAAGAAAT CTGGAAGAAA GCCGAAGAGG CTGTGAATGA AGTGAAGAGG 
CAGGCCATGT CTGAGCTGCA GAAAGCCGTG TCAGAGGCTG AGCGCAAGGC ACACGAGATG 
ATCAGCACAG AGCGAGCCAA GATGGAGCGC ACGGTGGCTG AGGCCAGGAG GCAAGCAGCA 
GAGGACGCGC TGTCAGTCAT CAACCAGCAG GAGGACTCCA GTGAGAGTTG CTGGAATTGT 
GGGCGCAAGG CGAGCGAGAC CTGCAGCGGC TGCAACACGG CGCGTTACTG CGGTTCCTTC 
TGCCAGCACA AAGACTGGGA AAAGCACCAC CACGTGTGTG GCCAGACACT GCAGGCCCAG 
CAGCAGGGTG AGACCCCTGC CACCGTCAGC TCTTCAGCCA CTCCCAGCAG CGGAGCTGGA 
AGTCCAGCAG ACACACCGTC TGCCGCCGCC ACTCCTCGCT CAGCCACACC CGGCACGCCC 
TCCACCGTGG AGAACACGCC GCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070244.2
CDS	393..2159
Translation	MVGISTSFQYRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	NM_001076776.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RUNX1 partner transcriptional co-repressor 1 (runx1t1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076776.4

ORF Insert Sequence:

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATCTCCTGA ACTGCATTAT GGATATGGTG GAGAAGACAC GTCGCTCATT GACAGTGTTG 
CGGAGGTGCC AGGAGACTGA CCGAGAAGAG CTCAACTACT GGATCCGGCG TTACAGCGAT 
GCTGAAGACC TTAAAAAAAC CAGCAGCACT AGCACCAGTC AGTCAAGACA ACAGAGTCCC 
GCTAGCCAAG AAGGCAATGC TTTAGAAGTA CATAGGGAGC TCCTTCACAG GCCAGTGTCT 
GGATACGTAC CTGAAGAAAT CTGGAAGAAA GCCGAAGAGG CTGTGAATGA AGTGAAGAGG 
CAGGCCATGT CTGAGCTGCA GAAAGCCGTG TCAGAGGCTG AGCGCAAGGC ACACGAGATG 
ATCAGCACAG AGCGAGCCAA GATGGAGCGC ACGGTGGCTG AGGCCAGGAG GCAAGCAGCA 
GAGGACGCGC TGTCAGTCAT CAACCAGCAG GAGGACTCCA GTGAGAGTTG CTGGAATTGT 
GGGCGCAAGG CGAGCGAGAC CTGCAGCGGC TGCAACACGG CGCGTTACTG CGGTTCCTTC 
TGCCAGCACA AAGACTGGGA AAAGCACCAC CACGTGTGTG GCCAGACACT GCAGGCCCAG 
CAGCAGGGTG AGACCCCTGC CACCGTCAGC TCTTCAGCCA CTCCCAGCAG CGGAGCTGGA 
AGTCCAGCAG ACACACCGTC TGCCGCCGCC ACTCCTCGCT CAGCCACACC CGGCACGCCC 
TCCACCGTGG AGAACACGCC GCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352274.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207763.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56084
Clone ID Related Accession (Same CDS sequence)	XM_017352274.1 , XM_017352274.2
Accession Version	XM_017352274.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein CBFA2T1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAAGAGGC TGTGAATGAA 
GTGAAGAGGC AGGCCATGTC TGAGCTGCAG AAAGCCGTGT CAGAGGCTGA GCGCAAGGCA 
CACGAGATGA TCAGCACAGA GCGAGCCAAG ATGGAGCGCA CGGTGGCTGA GGCCAGGAGG 
CAAGCAGCAG AGGACGCGCT GTCAGTCATC AACCAGCAGG AGGACTCCAG TGAGAGTTGC 
TGGAATTGTG GGCGCAAGGC GAGCGAGACC TGCAGCGGCT GCAACACGGC GCGTTACTGC 
GGTTCCTTCT GCCAGCACAA AGACTGGGAA AAGCACCACC ACGTGTGTGG CCAGACACTG 
CAGGCCCAGC AGCAGGGTGA GACCCCTGCC ACCGTCAGCT CTTCAGCCAC TCCCAGCAGC 
GGAGCTGGAA GTCCAGCAGA CACACCGTCT GCCGCCGCCA CTCCTCGCTC AGCCACACCC 
GGCACGCCCT CCACCGTGGA GAACACGCCG CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207763.1
CDS	478..2253
Translation	MVGISTSFQYRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352274.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352274.1

ORF Insert Sequence:

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAAGAGGC TGTGAATGAA 
GTGAAGAGGC AGGCCATGTC TGAGCTGCAG AAAGCCGTGT CAGAGGCTGA GCGCAAGGCA 
CACGAGATGA TCAGCACAGA GCGAGCCAAG ATGGAGCGCA CGGTGGCTGA GGCCAGGAGG 
CAAGCAGCAG AGGACGCGCT GTCAGTCATC AACCAGCAGG AGGACTCCAG TGAGAGTTGC 
TGGAATTGTG GGCGCAAGGC GAGCGAGACC TGCAGCGGCT GCAACACGGC GCGTTACTGC 
GGTTCCTTCT GCCAGCACAA AGACTGGGAA AAGCACCACC ACGTGTGTGG CCAGACACTG 
CAGGCCCAGC AGCAGGGTGA GACCCCTGCC ACCGTCAGCT CTTCAGCCAC TCCCAGCAGC 
GGAGCTGGAA GTCCAGCAGA CACACCGTCT GCCGCCGCCA CTCCTCGCTC AGCCACACCC 
GGCACGCCCT CCACCGTGGA GAACACGCCG CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352274.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207763.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa56084
Clone ID Related Accession (Same CDS sequence)	XM_017352274.1 , XM_017352274.2
Accession Version	XM_017352274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein CBFA2T1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAAGAGGC TGTGAATGAA 
GTGAAGAGGC AGGCCATGTC TGAGCTGCAG AAAGCCGTGT CAGAGGCTGA GCGCAAGGCA 
CACGAGATGA TCAGCACAGA GCGAGCCAAG ATGGAGCGCA CGGTGGCTGA GGCCAGGAGG 
CAAGCAGCAG AGGACGCGCT GTCAGTCATC AACCAGCAGG AGGACTCCAG TGAGAGTTGC 
TGGAATTGTG GGCGCAAGGC GAGCGAGACC TGCAGCGGCT GCAACACGGC GCGTTACTGC 
GGTTCCTTCT GCCAGCACAA AGACTGGGAA AAGCACCACC ACGTGTGTGG CCAGACACTG 
CAGGCCCAGC AGCAGGGTGA GACCCCTGCC ACCGTCAGCT CTTCAGCCAC TCCCAGCAGC 
GGAGCTGGAA GTCCAGCAGA CACACCGTCT GCCGCCGCCA CTCCTCGCTC AGCCACACCC 
GGCACGCCCT CCACCGTGGA GAACACGCCG CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207763.1
CDS	435..2210
Translation	MVGISTSFQYRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352274.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352274.2

ORF Insert Sequence:

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAAGAGGC TGTGAATGAA 
GTGAAGAGGC AGGCCATGTC TGAGCTGCAG AAAGCCGTGT CAGAGGCTGA GCGCAAGGCA 
CACGAGATGA TCAGCACAGA GCGAGCCAAG ATGGAGCGCA CGGTGGCTGA GGCCAGGAGG 
CAAGCAGCAG AGGACGCGCT GTCAGTCATC AACCAGCAGG AGGACTCCAG TGAGAGTTGC 
TGGAATTGTG GGCGCAAGGC GAGCGAGACC TGCAGCGGCT GCAACACGGC GCGTTACTGC 
GGTTCCTTCT GCCAGCACAA AGACTGGGAA AAGCACCACC ACGTGTGTGG CCAGACACTG 
CAGGCCCAGC AGCAGGGTGA GACCCCTGCC ACCGTCAGCT CTTCAGCCAC TCCCAGCAGC 
GGAGCTGGAA GTCCAGCAGA CACACCGTCT GCCGCCGCCA CTCCTCGCTC AGCCACACCC 
GGCACGCCCT CCACCGTGGA GAACACGCCG CGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352275.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207764.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.

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CloneID	ODa56085
Clone ID Related Accession (Same CDS sequence)	XM_017352275.1 , XM_017352275.2
Accession Version	XM_017352275.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein CBFA2T1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAGGCTGT GAATGAAGTG 
AAGAGGCAGG CCATGTCTGA GCTGCAGAAA GCCGTGTCAG AGGCTGAGCG CAAGGCACAC 
GAGATGATCA GCACAGAGCG AGCCAAGATG GAGCGCACGG TGGCTGAGGC CAGGAGGCAA 
GCAGCAGAGG ACGCGCTGTC AGTCATCAAC CAGCAGGAGG ACTCCAGTGA GAGTTGCTGG 
AATTGTGGGC GCAAGGCGAG CGAGACCTGC AGCGGCTGCA ACACGGCGCG TTACTGCGGT 
TCCTTCTGCC AGCACAAAGA CTGGGAAAAG CACCACCACG TGTGTGGCCA GACACTGCAG 
GCCCAGCAGC AGGGTGAGAC CCCTGCCACC GTCAGCTCTT CAGCCACTCC CAGCAGCGGA 
GCTGGAAGTC CAGCAGACAC ACCGTCTGCC GCCGCCACTC CTCGCTCAGC CACACCCGGC 
ACGCCCTCCA CCGTGGAGAA CACGCCGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207764.1
CDS	478..2250
Translation	MVGISTSFQYRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352275.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352275.1

ORF Insert Sequence:

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAGGCTGT GAATGAAGTG 
AAGAGGCAGG CCATGTCTGA GCTGCAGAAA GCCGTGTCAG AGGCTGAGCG CAAGGCACAC 
GAGATGATCA GCACAGAGCG AGCCAAGATG GAGCGCACGG TGGCTGAGGC CAGGAGGCAA 
GCAGCAGAGG ACGCGCTGTC AGTCATCAAC CAGCAGGAGG ACTCCAGTGA GAGTTGCTGG 
AATTGTGGGC GCAAGGCGAG CGAGACCTGC AGCGGCTGCA ACACGGCGCG TTACTGCGGT 
TCCTTCTGCC AGCACAAAGA CTGGGAAAAG CACCACCACG TGTGTGGCCA GACACTGCAG 
GCCCAGCAGC AGGGTGAGAC CCCTGCCACC GTCAGCTCTT CAGCCACTCC CAGCAGCGGA 
GCTGGAAGTC CAGCAGACAC ACCGTCTGCC GCCGCCACTC CTCGCTCAGC CACACCCGGC 
ACGCCCTCCA CCGTGGAGAA CACGCCGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352275.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207764.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.

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CloneID	ODa56085
Clone ID Related Accession (Same CDS sequence)	XM_017352275.1 , XM_017352275.2
Accession Version	XM_017352275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein CBFA2T1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAGGCTGT GAATGAAGTG 
AAGAGGCAGG CCATGTCTGA GCTGCAGAAA GCCGTGTCAG AGGCTGAGCG CAAGGCACAC 
GAGATGATCA GCACAGAGCG AGCCAAGATG GAGCGCACGG TGGCTGAGGC CAGGAGGCAA 
GCAGCAGAGG ACGCGCTGTC AGTCATCAAC CAGCAGGAGG ACTCCAGTGA GAGTTGCTGG 
AATTGTGGGC GCAAGGCGAG CGAGACCTGC AGCGGCTGCA ACACGGCGCG TTACTGCGGT 
TCCTTCTGCC AGCACAAAGA CTGGGAAAAG CACCACCACG TGTGTGGCCA GACACTGCAG 
GCCCAGCAGC AGGGTGAGAC CCCTGCCACC GTCAGCTCTT CAGCCACTCC CAGCAGCGGA 
GCTGGAAGTC CAGCAGACAC ACCGTCTGCC GCCGCCACTC CTCGCTCAGC CACACCCGGC 
ACGCCCTCCA CCGTGGAGAA CACGCCGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207764.1
CDS	435..2207
Translation	MVGISTSFQYRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352275.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352275.2

ORF Insert Sequence:

ATGGTGGGAA TTTCTACCTC TTTTCAGTAT CGCACTGAGA AGCGCTCCAC CATGCCCGAC 
TCACCTGCTG ATGTGAAAAC ACAGTCCAGG TTAACTCCGC CCACCATGCC TCCTCCCCCC 
ACCACACAGG GAGCTCCCAG GACCAGCTCC TTCACCCCCA CAGCATTAAC GAATGGCACC 
AGTCACTCTC CCACTGCACT AAATGGAGCT CCATCTCCAC CCAATGGCTT CAGCAATGGG 
CCTTCGTCAT CATCTTCATC CTCGCTGGCC AATCAACAGC TGCCACCGGC CTGTGGAGCA 
AGGCAGCTGA GCAAGCTCAA ACGCTTCCTC ACAACACTAC AGCAGTTCGG CAATGACATC 
TCGCCTGAGA TAGGAGAACG AGTTCGCACG CTGGTCTTAG GACTTGTGAA CTCCACTCTA 
ACCATTGAGG AATTCCACTC CAAACTGCAA GAAGCGACCA ACTTCCCACT CCGACCGTTT 
GTCATCCCGT TTCTGAAGGC CAATCTCCCG CTACTGCAGA GGGAACTGGT GCACTGTGCA 
CGGCTGGCCA AGCAGAACCC GGCTCAATAC CTAGCCCAAC ATGAGCAGCT CCTGCTGGAC 
ACAAGCACCA CGTCGCCTGT CGACTCGTCT GAACTCTTGA TGGACGTCAA CGAGAATGGC 
AAGAGAAGAA CACCTGACAG AACCAAAGAG AACGGCTTTG AGCGGGAGCC CCTTCATCCT 
GAGCACCCCA ACAAGAGGCC CTGCACTATA AGCCCGGGAC AGCGCTTCAG CCCCTCCAAC 
GGGCTCTCCT ACCAGCCCAA TGGCCTTCCA CACCCCACAC CACCCCCTCC ACAGCACTAC 
CGTCTGGACG ACATGGCTAT CGCACACCAC TACAGAGATT CCTATCGTCA CCCCAACCAC 
CGCGAGCTCC GCGACCGCCC TCGGCCACTG GGGATGCACG GCAGCAGCAC ACGCCAGGAG 
GAAGTCATAG ATCACAGACT CACAGACAGA GAATGGGCAG AAGAGTGGAA GCACCTTGAC 
CATGTAAGAA AACTCCTGAA CTGCATTATG GATATGGTGG AGAAGACACG TCGCTCATTG 
ACAGTGTTGC GGAGGTGCCA GGAGACTGAC CGAGAAGAGC TCAACTACTG GATCCGGCGT 
TACAGCGATG CTGAAGACCT TAAAAAAACC AGCAGCACTA GCACCAGTCA GTCAAGACAA 
CAGAGTCCCG CTAGCCAAGA AGGCAATGCT TTAGAAGTAC ATAGGGAGCT CCTTCACAGG 
CCAGTGTCTG GATACGTACC TGAAGAAATC TGGAAGAAAG CCGAGGCTGT GAATGAAGTG 
AAGAGGCAGG CCATGTCTGA GCTGCAGAAA GCCGTGTCAG AGGCTGAGCG CAAGGCACAC 
GAGATGATCA GCACAGAGCG AGCCAAGATG GAGCGCACGG TGGCTGAGGC CAGGAGGCAA 
GCAGCAGAGG ACGCGCTGTC AGTCATCAAC CAGCAGGAGG ACTCCAGTGA GAGTTGCTGG 
AATTGTGGGC GCAAGGCGAG CGAGACCTGC AGCGGCTGCA ACACGGCGCG TTACTGCGGT 
TCCTTCTGCC AGCACAAAGA CTGGGAAAAG CACCACCACG TGTGTGGCCA GACACTGCAG 
GCCCAGCAGC AGGGTGAGAC CCCTGCCACC GTCAGCTCTT CAGCCACTCC CAGCAGCGGA 
GCTGGAAGTC CAGCAGACAC ACCGTCTGCC GCCGCCACTC CTCGCTCAGC CACACCCGGC 
ACGCCCTCCA CCGTGGAGAA CACGCCGCGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352276.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207765.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa56086
Clone ID Related Accession (Same CDS sequence)	XM_017352276.1 , XM_017352276.2
Accession Version	XM_017352276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein CBFA2T1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCAG ATCGCACTGA GAAGCGCTCC ACCATGCCCG ACTCACCTGC TGATGTGAAA 
ACACAGTCCA GGTTAACTCC GCCCACCATG CCTCCTCCCC CCACCACACA GGGAGCTCCC 
AGGACCAGCT CCTTCACCCC CACAGCATTA ACGAATGGCA CCAGTCACTC TCCCACTGCA 
CTAAATGGAG CTCCATCTCC ACCCAATGGC TTCAGCAATG GGCCTTCGTC ATCATCTTCA 
TCCTCGCTGG CCAATCAACA GCTGCCACCG GCCTGTGGAG CAAGGCAGCT GAGCAAGCTC 
AAACGCTTCC TCACAACACT ACAGCAGTTC GGCAATGACA TCTCGCCTGA GATAGGAGAA 
CGAGTTCGCA CGCTGGTCTT AGGACTTGTG AACTCCACTC TAACCATTGA GGAATTCCAC 
TCCAAACTGC AAGAAGCGAC CAACTTCCCA CTCCGACCGT TTGTCATCCC GTTTCTGAAG 
GCCAATCTCC CGCTACTGCA GAGGGAACTG GTGCACTGTG CACGGCTGGC CAAGCAGAAC 
CCGGCTCAAT ACCTAGCCCA ACATGAGCAG CTCCTGCTGG ACACAAGCAC CACGTCGCCT 
GTCGACTCGT CTGAACTCTT GATGGACGTC AACGAGAATG GCAAGAGAAG AACACCTGAC 
AGAACCAAAG AGAACGGCTT TGAGCGGGAG CCCCTTCATC CTGAGCACCC CAACAAGAGG 
CCCTGCACTA TAAGCCCGGG ACAGCGCTTC AGCCCCTCCA ACGGGCTCTC CTACCAGCCC 
AATGGCCTTC CACACCCCAC ACCACCCCCT CCACAGCACT ACCGTCTGGA CGACATGGCT 
ATCGCACACC ACTACAGAGA TTCCTATCGT CACCCCAACC ACCGCGAGCT CCGCGACCGC 
CCTCGGCCAC TGGGGATGCA CGGCAGCAGC ACACGCCAGG AGGAAGTCAT AGATCACAGA 
CTCACAGACA GAGAATGGGC AGAAGAGTGG AAGCACCTTG ACCATGTAAG AAAACTCCTG 
AACTGCATTA TGGATATGGT GGAGAAGACA CGTCGCTCAT TGACAGTGTT GCGGAGGTGC 
CAGGAGACTG ACCGAGAAGA GCTCAACTAC TGGATCCGGC GTTACAGCGA TGCTGAAGAC 
CTTAAAAAAA CCAGCAGCAC TAGCACCAGT CAGTCAAGAC AACAGAGTCC CGCTAGCCAA 
GAAGGCAATG CTTTAGAAGT ACATAGGGAG CTCCTTCACA GGCCAGTGTC TGGATACGTA 
CCTGAAGAAA TCTGGAAGAA AGCCGAAGAG GCTGTGAATG AAGTGAAGAG GCAGGCCATG 
TCTGAGCTGC AGAAAGCCGT GTCAGAGGCT GAGCGCAAGG CACACGAGAT GATCAGCACA 
GAGCGAGCCA AGATGGAGCG CACGGTGGCT GAGGCCAGGA GGCAAGCAGC AGAGGACGCG 
CTGTCAGTCA TCAACCAGCA GGAGGACTCC AGTGAGAGTT GCTGGAATTG TGGGCGCAAG 
GCGAGCGAGA CCTGCAGCGG CTGCAACACG GCGCGTTACT GCGGTTCCTT CTGCCAGCAC 
AAAGACTGGG AAAAGCACCA CCACGTGTGT GGCCAGACAC TGCAGGCCCA GCAGCAGGGT 
GAGACCCCTG CCACCGTCAG CTCTTCAGCC ACTCCCAGCA GCGGAGCTGG AAGTCCAGCA 
GACACACCGT CTGCCGCCGC CACTCCTCGC TCAGCCACAC CCGGCACGCC CTCCACCGTG 
GAGAACACGC CGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207765.1
CDS	357..2114
Translation	MMPDRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352276.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352276.1

ORF Insert Sequence:

ATGATGCCAG ATCGCACTGA GAAGCGCTCC ACCATGCCCG ACTCACCTGC TGATGTGAAA 
ACACAGTCCA GGTTAACTCC GCCCACCATG CCTCCTCCCC CCACCACACA GGGAGCTCCC 
AGGACCAGCT CCTTCACCCC CACAGCATTA ACGAATGGCA CCAGTCACTC TCCCACTGCA 
CTAAATGGAG CTCCATCTCC ACCCAATGGC TTCAGCAATG GGCCTTCGTC ATCATCTTCA 
TCCTCGCTGG CCAATCAACA GCTGCCACCG GCCTGTGGAG CAAGGCAGCT GAGCAAGCTC 
AAACGCTTCC TCACAACACT ACAGCAGTTC GGCAATGACA TCTCGCCTGA GATAGGAGAA 
CGAGTTCGCA CGCTGGTCTT AGGACTTGTG AACTCCACTC TAACCATTGA GGAATTCCAC 
TCCAAACTGC AAGAAGCGAC CAACTTCCCA CTCCGACCGT TTGTCATCCC GTTTCTGAAG 
GCCAATCTCC CGCTACTGCA GAGGGAACTG GTGCACTGTG CACGGCTGGC CAAGCAGAAC 
CCGGCTCAAT ACCTAGCCCA ACATGAGCAG CTCCTGCTGG ACACAAGCAC CACGTCGCCT 
GTCGACTCGT CTGAACTCTT GATGGACGTC AACGAGAATG GCAAGAGAAG AACACCTGAC 
AGAACCAAAG AGAACGGCTT TGAGCGGGAG CCCCTTCATC CTGAGCACCC CAACAAGAGG 
CCCTGCACTA TAAGCCCGGG ACAGCGCTTC AGCCCCTCCA ACGGGCTCTC CTACCAGCCC 
AATGGCCTTC CACACCCCAC ACCACCCCCT CCACAGCACT ACCGTCTGGA CGACATGGCT 
ATCGCACACC ACTACAGAGA TTCCTATCGT CACCCCAACC ACCGCGAGCT CCGCGACCGC 
CCTCGGCCAC TGGGGATGCA CGGCAGCAGC ACACGCCAGG AGGAAGTCAT AGATCACAGA 
CTCACAGACA GAGAATGGGC AGAAGAGTGG AAGCACCTTG ACCATGTAAG AAAACTCCTG 
AACTGCATTA TGGATATGGT GGAGAAGACA CGTCGCTCAT TGACAGTGTT GCGGAGGTGC 
CAGGAGACTG ACCGAGAAGA GCTCAACTAC TGGATCCGGC GTTACAGCGA TGCTGAAGAC 
CTTAAAAAAA CCAGCAGCAC TAGCACCAGT CAGTCAAGAC AACAGAGTCC CGCTAGCCAA 
GAAGGCAATG CTTTAGAAGT ACATAGGGAG CTCCTTCACA GGCCAGTGTC TGGATACGTA 
CCTGAAGAAA TCTGGAAGAA AGCCGAAGAG GCTGTGAATG AAGTGAAGAG GCAGGCCATG 
TCTGAGCTGC AGAAAGCCGT GTCAGAGGCT GAGCGCAAGG CACACGAGAT GATCAGCACA 
GAGCGAGCCA AGATGGAGCG CACGGTGGCT GAGGCCAGGA GGCAAGCAGC AGAGGACGCG 
CTGTCAGTCA TCAACCAGCA GGAGGACTCC AGTGAGAGTT GCTGGAATTG TGGGCGCAAG 
GCGAGCGAGA CCTGCAGCGG CTGCAACACG GCGCGTTACT GCGGTTCCTT CTGCCAGCAC 
AAAGACTGGG AAAAGCACCA CCACGTGTGT GGCCAGACAC TGCAGGCCCA GCAGCAGGGT 
GAGACCCCTG CCACCGTCAG CTCTTCAGCC ACTCCCAGCA GCGGAGCTGG AAGTCCAGCA 
GACACACCGT CTGCCGCCGC CACTCCTCGC TCAGCCACAC CCGGCACGCC CTCCACCGTG 
GAGAACACGC CGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

runx1t1 ( XM_017352276.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207765.1 Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.

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CloneID	ODa56086
Clone ID Related Accession (Same CDS sequence)	XM_017352276.1 , XM_017352276.2
Accession Version	XM_017352276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein CBFA2T1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCCAG ATCGCACTGA GAAGCGCTCC ACCATGCCCG ACTCACCTGC TGATGTGAAA 
ACACAGTCCA GGTTAACTCC GCCCACCATG CCTCCTCCCC CCACCACACA GGGAGCTCCC 
AGGACCAGCT CCTTCACCCC CACAGCATTA ACGAATGGCA CCAGTCACTC TCCCACTGCA 
CTAAATGGAG CTCCATCTCC ACCCAATGGC TTCAGCAATG GGCCTTCGTC ATCATCTTCA 
TCCTCGCTGG CCAATCAACA GCTGCCACCG GCCTGTGGAG CAAGGCAGCT GAGCAAGCTC 
AAACGCTTCC TCACAACACT ACAGCAGTTC GGCAATGACA TCTCGCCTGA GATAGGAGAA 
CGAGTTCGCA CGCTGGTCTT AGGACTTGTG AACTCCACTC TAACCATTGA GGAATTCCAC 
TCCAAACTGC AAGAAGCGAC CAACTTCCCA CTCCGACCGT TTGTCATCCC GTTTCTGAAG 
GCCAATCTCC CGCTACTGCA GAGGGAACTG GTGCACTGTG CACGGCTGGC CAAGCAGAAC 
CCGGCTCAAT ACCTAGCCCA ACATGAGCAG CTCCTGCTGG ACACAAGCAC CACGTCGCCT 
GTCGACTCGT CTGAACTCTT GATGGACGTC AACGAGAATG GCAAGAGAAG AACACCTGAC 
AGAACCAAAG AGAACGGCTT TGAGCGGGAG CCCCTTCATC CTGAGCACCC CAACAAGAGG 
CCCTGCACTA TAAGCCCGGG ACAGCGCTTC AGCCCCTCCA ACGGGCTCTC CTACCAGCCC 
AATGGCCTTC CACACCCCAC ACCACCCCCT CCACAGCACT ACCGTCTGGA CGACATGGCT 
ATCGCACACC ACTACAGAGA TTCCTATCGT CACCCCAACC ACCGCGAGCT CCGCGACCGC 
CCTCGGCCAC TGGGGATGCA CGGCAGCAGC ACACGCCAGG AGGAAGTCAT AGATCACAGA 
CTCACAGACA GAGAATGGGC AGAAGAGTGG AAGCACCTTG ACCATGTAAG AAAACTCCTG 
AACTGCATTA TGGATATGGT GGAGAAGACA CGTCGCTCAT TGACAGTGTT GCGGAGGTGC 
CAGGAGACTG ACCGAGAAGA GCTCAACTAC TGGATCCGGC GTTACAGCGA TGCTGAAGAC 
CTTAAAAAAA CCAGCAGCAC TAGCACCAGT CAGTCAAGAC AACAGAGTCC CGCTAGCCAA 
GAAGGCAATG CTTTAGAAGT ACATAGGGAG CTCCTTCACA GGCCAGTGTC TGGATACGTA 
CCTGAAGAAA TCTGGAAGAA AGCCGAAGAG GCTGTGAATG AAGTGAAGAG GCAGGCCATG 
TCTGAGCTGC AGAAAGCCGT GTCAGAGGCT GAGCGCAAGG CACACGAGAT GATCAGCACA 
GAGCGAGCCA AGATGGAGCG CACGGTGGCT GAGGCCAGGA GGCAAGCAGC AGAGGACGCG 
CTGTCAGTCA TCAACCAGCA GGAGGACTCC AGTGAGAGTT GCTGGAATTG TGGGCGCAAG 
GCGAGCGAGA CCTGCAGCGG CTGCAACACG GCGCGTTACT GCGGTTCCTT CTGCCAGCAC 
AAAGACTGGG AAAAGCACCA CCACGTGTGT GGCCAGACAC TGCAGGCCCA GCAGCAGGGT 
GAGACCCCTG CCACCGTCAG CTCTTCAGCC ACTCCCAGCA GCGGAGCTGG AAGTCCAGCA 
GACACACCGT CTGCCGCCGC CACTCCTCGC TCAGCCACAC CCGGCACGCC CTCCACCGTG 
GAGAACACGC CGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207765.1
CDS	344..2101
Translation	MMPDRTEKRSTMPDSPADVKTQSRLTPPTMPPPPTTQGAPRTSSFTPTALTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELVHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLMDVNENGKRRTPDRTKENGFEREPLHPEHPNKRPCTISPGQRFSPSNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPNHRELRDRPRPLGMHGSSTRQEEVIDHRLTDREWAEEWKHLDHVRKLLNCIMDMVEKTRRSLTVLRRCQETDREELNYWIRRYSDAEDLKKTSSTSTSQSRQQSPASQEGNALEVHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISTERAKMERTVAEARRQAAEDALSVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQGETPATVSSSATPSSGAGSPADTPSAAATPRSATPGTPSTVENTPR

Target ORF information:

RefSeq Version	XM_017352276.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (runx1t1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352276.2

ORF Insert Sequence:

ATGATGCCAG ATCGCACTGA GAAGCGCTCC ACCATGCCCG ACTCACCTGC TGATGTGAAA 
ACACAGTCCA GGTTAACTCC GCCCACCATG CCTCCTCCCC CCACCACACA GGGAGCTCCC 
AGGACCAGCT CCTTCACCCC CACAGCATTA ACGAATGGCA CCAGTCACTC TCCCACTGCA 
CTAAATGGAG CTCCATCTCC ACCCAATGGC TTCAGCAATG GGCCTTCGTC ATCATCTTCA 
TCCTCGCTGG CCAATCAACA GCTGCCACCG GCCTGTGGAG CAAGGCAGCT GAGCAAGCTC 
AAACGCTTCC TCACAACACT ACAGCAGTTC GGCAATGACA TCTCGCCTGA GATAGGAGAA 
CGAGTTCGCA CGCTGGTCTT AGGACTTGTG AACTCCACTC TAACCATTGA GGAATTCCAC 
TCCAAACTGC AAGAAGCGAC CAACTTCCCA CTCCGACCGT TTGTCATCCC GTTTCTGAAG 
GCCAATCTCC CGCTACTGCA GAGGGAACTG GTGCACTGTG CACGGCTGGC CAAGCAGAAC 
CCGGCTCAAT ACCTAGCCCA ACATGAGCAG CTCCTGCTGG ACACAAGCAC CACGTCGCCT 
GTCGACTCGT CTGAACTCTT GATGGACGTC AACGAGAATG GCAAGAGAAG AACACCTGAC 
AGAACCAAAG AGAACGGCTT TGAGCGGGAG CCCCTTCATC CTGAGCACCC CAACAAGAGG 
CCCTGCACTA TAAGCCCGGG ACAGCGCTTC AGCCCCTCCA ACGGGCTCTC CTACCAGCCC 
AATGGCCTTC CACACCCCAC ACCACCCCCT CCACAGCACT ACCGTCTGGA CGACATGGCT 
ATCGCACACC ACTACAGAGA TTCCTATCGT CACCCCAACC ACCGCGAGCT CCGCGACCGC 
CCTCGGCCAC TGGGGATGCA CGGCAGCAGC ACACGCCAGG AGGAAGTCAT AGATCACAGA 
CTCACAGACA GAGAATGGGC AGAAGAGTGG AAGCACCTTG ACCATGTAAG AAAACTCCTG 
AACTGCATTA TGGATATGGT GGAGAAGACA CGTCGCTCAT TGACAGTGTT GCGGAGGTGC 
CAGGAGACTG ACCGAGAAGA GCTCAACTAC TGGATCCGGC GTTACAGCGA TGCTGAAGAC 
CTTAAAAAAA CCAGCAGCAC TAGCACCAGT CAGTCAAGAC AACAGAGTCC CGCTAGCCAA 
GAAGGCAATG CTTTAGAAGT ACATAGGGAG CTCCTTCACA GGCCAGTGTC TGGATACGTA 
CCTGAAGAAA TCTGGAAGAA AGCCGAAGAG GCTGTGAATG AAGTGAAGAG GCAGGCCATG 
TCTGAGCTGC AGAAAGCCGT GTCAGAGGCT GAGCGCAAGG CACACGAGAT GATCAGCACA 
GAGCGAGCCA AGATGGAGCG CACGGTGGCT GAGGCCAGGA GGCAAGCAGC AGAGGACGCG 
CTGTCAGTCA TCAACCAGCA GGAGGACTCC AGTGAGAGTT GCTGGAATTG TGGGCGCAAG 
GCGAGCGAGA CCTGCAGCGG CTGCAACACG GCGCGTTACT GCGGTTCCTT CTGCCAGCAC 
AAAGACTGGG AAAAGCACCA CCACGTGTGT GGCCAGACAC TGCAGGCCCA GCAGCAGGGT 
GAGACCCCTG CCACCGTCAG CTCTTCAGCC ACTCCCAGCA GCGGAGCTGG AAGTCCAGCA 
GACACACCGT CTGCCGCCGC CACTCCTCGC TCAGCCACAC CCGGCACGCC CTCCACCGTG 
GAGAACACGC CGCGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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runx1t1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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