ncl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ncl
Entrez Gene ID	767714
Full Name	nucleolin
Synonyms	wu:fa12d03,zgc:152810
General protein information	Preferred Names nucleolin Names nucleolin fa12d03
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170548.4	XP_005170605.1	nucleolin isoform X2
XM_005170547.3	XP_005170604.1	nucleolin isoform X2
XM_009296514.2	XP_009294789.1	nucleolin isoform X3
XM_009296515.2	XP_009294790.1	nucleolin isoform X4
XM_009296513.2	XP_009294788.1	nucleolin isoform X1
NM_001076652.2	NP_001070120.2	nucleolin
XM_005170548.3	XP_005170605.1	nucleolin isoform X5
XM_005170547.4	XP_005170604.1	nucleolin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cytoplasmic polyadenylation-mediated translational control of maternal mRNAs directs maternal-to-zygotic transition.
Winata CL, ?api?ski M, Pryszcz L, Vaz C, Bin Ismail MH, Nama S, Hajan HS, Lee SGP, Korzh V, Sampath P, Tanavde V, Mathavan S
Development (Cambridge, England)145(1)(2018 Jan)

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Chakraborty A, Uechi T, Nakajima Y, Gazda HT, O'Donohue MF, Gleizes PE, Kenmochi N
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Systems proteomics of cardiac chromatin identifies nucleolin as a regulator of growth and cellular plasticity in cardiomyocytes.
Monte E, Mouillesseaux K, Chen H, Kimball T, Ren S, Wang Y, Chen JN, Vondriska TM, Franklin S
American journal of physiology. Heart and circulatory physiology305(11)H1624-38(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that during heart disease nucleolin is reactivated to mobilize conserved aspects of chromatin remodeling and gene expression.
Title: Systems proteomics of cardiac chromatin identifies nucleolin as a regulator of growth and cellular plasticity in cardiomyocytes.

The following ncl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ncl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38906	NM_001076652.2 Latest version!	Danio rerio nucleolin (ncl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa27215	XM_005170547.4 Latest version!	Danio rerio nucleolin (ncl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa27216	XM_005170548.4 Latest version!	Danio rerio nucleolin (ncl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
ODa46518	XM_009296513.2 Latest version!	Danio rerio nucleolin (ncl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa46519	XM_009296514.2 Latest version!	Danio rerio nucleolin (ncl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa46520	XM_009296515.2 Latest version!	Danio rerio nucleolin (ncl), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
ODa27216	XM_005170548.3	Danio rerio nucleolin (ncl), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
ODa27215	XM_005170547.3	Danio rerio nucleolin (ncl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ncl ( NM_001076652.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070120.2 Danio rerio nucleolin (ncl), mRNA.

Price & Availability↑

CloneID	ODa38906
Clone ID Related Accession (Same CDS sequence)	NM_001076652.2
Accession Version	NM_001076652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	nucleolin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU539057.4 and CU469540.4. On or before Mar 26, 2009 this sequence version replaced NM_001003839.1, NM_001076652.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC124135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06645146, SAMN06645147 [ECO:0000348] ##Evidence-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGAAGAT GAATCTGAAG AGGAAGAAGC TCCGCCTCCT 
AAAAAAGCTG CAGCAGCGGC TAAACCTGCA GCTAAAGCAC CTGCAGCCAA AGAGGAGTCA 
GATGAAGAGG AGGATGATGA CGAAGAGGAG GATGATTCAG AAGAGGAGGA AATGGACACA 
ACACCTGCGC CCAAAGCCAA GAAAGCAGGA ATGGTGAAGG CCAAGGAGGA GTCTGAGGAA 
GAAGATGACG ACGATGAGGA AGATGATGAT GATGAGGAGG AAGAAGAAAC CCCGGCCACT 
CCAGCTAAGA GGAAAGCCGA GACCAAAAAA GAGAAGGGAA CGCCGCCGGC TAAGAAGGCT 
AAAACCGATG GCGAGGGTTT CAGTCTTTTT TTGGGCAACC TGAACAACAA TAAAGACTTT 
GACGAACTGA AGTCGGCTAT CTCAAAGTTC TTCTCAAAGG AGGGCCTTGA AATTCAGGAT 
GTGCGACTTG GTGGAACAAA GAAATTCGGC TACGTTGACT TCGCCTCAGA GGAAGAGTTG 
CAGAAGGCTT TAGAGCTCAA TGGCAAGAAG TTATTGGGTC AGCCAGTCAA ACTGGACAAA 
GCCAGAAGCA AAGAGAACTC GCAGGAAAAC AAGAAAGAGA GAGACGCCCG CACTTTATTC 
GTGAAGAATC TTCCATACTC AATAACACAA GACGACCTGA GGGAAATCTT TGACCAGGCT 
GTAGATATCA GAGTACCGAT GGGCAACACC GGCACTAGCA GAGGAATCGC CTACATTGAG 
TTCAAGACCG AGGCCATTGC AGAGAAGGCT TTAGAGGAGG CGCAGGGCTC AGATGTTCAG 
GGACGCTCCA TCATCGTCGA CTTCACAGGA GACAAGAGTC GGCAGGGCGG CAGAGGTGCT 
CCGTCTGCAA GTAAAGTGCT GGTGGTGAAC AACCTGGCGT TCAGTGCGAG TGAAGACTCC 
CTCCAGAGTG TGTTCGAGAA GGCCGTGTCC ATCAGAATAC CACAGAACAA CGGCAGACCC 
AAAGGATATG CGTTTGTGGA GTTTGAGAAT GTGGAGGACT CCAAGGAGGC GCTGGAGAAC 
TGCAACAACA CAGATATCGA GGGCAGGAGT ATTAGATTAG AGTACAGCCA GAACGACAGA 
GAAAGAGGCG GCGGAGGAAG AGGAAACTCT GGACCTACGA AAACCCTGTT TGTCAAAGGC 
CTGTCAGACG ACACCACAGA TCAGACTCTG AAAGACTCTT TTGATGGAGC CATCGCAGCC 
AGAATCGCCA CAGACAGAGA CACCGGATCA TCGAAAGGGT TTGGTTTTGT AGACTTCGAT 
AACGAGCAAG ACTGCAAGGC AGCTAAAGAA GCCATGGATG ACGGAGAGAT CGACGGAAAC 
AAAGTGACTC TGGACTACGC CAAGCCCAAA GGAGAAGGTG GACGAGGAGG ATTCGGCGGA 
GGAAGAGGAG GCTTTGGAGG ACGAGGCGGC GGAAGGGGTG GATTTGGAGG TAGAGGAGGA 
GGAGGACGAG GTGGCGGATT CAGGGGAGGA CGAGGAGGAA GAGGCGGCGG CGGAGGATTC 
AGAGGAGGAA GAGGAGGAGG AGGCCGAGGT GGATTCGGGG ACAGACCACA AGGAAAGAAG 
ATCAAGTTTG ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070120.2
CDS	135..2252
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDDESEEEAPPPKKAPAKATPAKATPAKKAAPVKKAAPAEESEDDDDDEDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	NM_001076652.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleolin (ncl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076652.2

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGAAGAT GAATCTGAAG AGGAAGAAGC TCCGCCTCCT 
AAAAAAGCTG CAGCAGCGGC TAAACCTGCA GCTAAAGCAC CTGCAGCCAA AGAGGAGTCA 
GATGAAGAGG AGGATGATGA CGAAGAGGAG GATGATTCAG AAGAGGAGGA AATGGACACA 
ACACCTGCGC CCAAAGCCAA GAAAGCAGGA ATGGTGAAGG CCAAGGAGGA GTCTGAGGAA 
GAAGATGACG ACGATGAGGA AGATGATGAT GATGAGGAGG AAGAAGAAAC CCCGGCCACT 
CCAGCTAAGA GGAAAGCCGA GACCAAAAAA GAGAAGGGAA CGCCGCCGGC TAAGAAGGCT 
AAAACCGATG GCGAGGGTTT CAGTCTTTTT TTGGGCAACC TGAACAACAA TAAAGACTTT 
GACGAACTGA AGTCGGCTAT CTCAAAGTTC TTCTCAAAGG AGGGCCTTGA AATTCAGGAT 
GTGCGACTTG GTGGAACAAA GAAATTCGGC TACGTTGACT TCGCCTCAGA GGAAGAGTTG 
CAGAAGGCTT TAGAGCTCAA TGGCAAGAAG TTATTGGGTC AGCCAGTCAA ACTGGACAAA 
GCCAGAAGCA AAGAGAACTC GCAGGAAAAC AAGAAAGAGA GAGACGCCCG CACTTTATTC 
GTGAAGAATC TTCCATACTC AATAACACAA GACGACCTGA GGGAAATCTT TGACCAGGCT 
GTAGATATCA GAGTACCGAT GGGCAACACC GGCACTAGCA GAGGAATCGC CTACATTGAG 
TTCAAGACCG AGGCCATTGC AGAGAAGGCT TTAGAGGAGG CGCAGGGCTC AGATGTTCAG 
GGACGCTCCA TCATCGTCGA CTTCACAGGA GACAAGAGTC GGCAGGGCGG CAGAGGTGCT 
CCGTCTGCAA GTAAAGTGCT GGTGGTGAAC AACCTGGCGT TCAGTGCGAG TGAAGACTCC 
CTCCAGAGTG TGTTCGAGAA GGCCGTGTCC ATCAGAATAC CACAGAACAA CGGCAGACCC 
AAAGGATATG CGTTTGTGGA GTTTGAGAAT GTGGAGGACT CCAAGGAGGC GCTGGAGAAC 
TGCAACAACA CAGATATCGA GGGCAGGAGT ATTAGATTAG AGTACAGCCA GAACGACAGA 
GAAAGAGGCG GCGGAGGAAG AGGAAACTCT GGACCTACGA AAACCCTGTT TGTCAAAGGC 
CTGTCAGACG ACACCACAGA TCAGACTCTG AAAGACTCTT TTGATGGAGC CATCGCAGCC 
AGAATCGCCA CAGACAGAGA CACCGGATCA TCGAAAGGGT TTGGTTTTGT AGACTTCGAT 
AACGAGCAAG ACTGCAAGGC AGCTAAAGAA GCCATGGATG ACGGAGAGAT CGACGGAAAC 
AAAGTGACTC TGGACTACGC CAAGCCCAAA GGAGAAGGTG GACGAGGAGG ATTCGGCGGA 
GGAAGAGGAG GCTTTGGAGG ACGAGGCGGC GGAAGGGGTG GATTTGGAGG TAGAGGAGGA 
GGAGGACGAG GTGGCGGATT CAGGGGAGGA CGAGGAGGAA GAGGCGGCGG CGGAGGATTC 
AGAGGAGGAA GAGGAGGAGG AGGCCGAGGT GGATTCGGGG ACAGACCACA AGGAAAGAAG 
ATCAAGTTTG ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_005170547.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170604.1 Danio rerio nucleolin (ncl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27215
Clone ID Related Accession (Same CDS sequence)	XM_005170547.3 , XM_005170547.4
Accession Version	XM_005170547.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170604.1
CDS	130..2244
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDDESEEEAPPPKKAPAKATPAKATPAKKAAPVKKAAPAEESEDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_005170547.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170547.3

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_005170547.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170604.1 Danio rerio nucleolin (ncl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27215
Clone ID Related Accession (Same CDS sequence)	XM_005170547.3 , XM_005170547.4
Accession Version	XM_005170547.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleolin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170547.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170604.1
CDS	136..2250
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDDESEEEAPPPKKAPAKATPAKATPAKKAAPVKKAAPAEESEDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_005170547.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170547.4

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGATG AATCCGAGGA AGAGGCTCCA CCTCCCAAGA AGGCTCCTGC CAAAGCTACA 
CCAGCTAAAG CCACACCAGC CAAGAAAGCA GCTCCCGTGA AGAAAGCAGC TCCTGCTGAG 
GAGTCTGAGG ATGACGATGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_005170548.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170605.1 Danio rerio nucleolin (ncl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27216
Clone ID Related Accession (Same CDS sequence)	XM_005170548.4 , XM_005170548.3
Accession Version	XM_005170548.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleolin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170548.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGAAT CTGAAGAGGA AGAAGCTCCG CCTCCTAAAA AAGCTGCAGC AGCGGCTAAA 
CCTGCAGCTA AAGCACCTGC AGCCAAAGAG GAGTCAGATG AAGAGGAGGA TGATGACGAA 
GAGGAGGATG ATTCAGAAGA GGAGGAAATG GACACAACAC CTGCGCCCAA AGCCAAGAAA 
GCAGGAATGG TGAAGGCCAA GGAGGAGTCT GAGGAAGAAG ATGACGACGA TGAGGAAGAT 
GATGATGATG AGGAGGAAGA AGAAACCCCG GCCACTCCAG CTAAGAGGAA AGCCGAGACC 
AAAAAAGAGA AGGGAACGCC GCCGGCTAAG AAGGCTAAAA CCGATGGCGA GGGTTTCAGT 
CTTTTTTTGG GCAACCTGAA CAACAATAAA GACTTTGACG AACTGAAGTC GGCTATCTCA 
AAGTTCTTCT CAAAGGAGGG CCTTGAAATT CAGGATGTGC GACTTGGTGG AACAAAGAAA 
TTCGGCTACG TTGACTTCGC CTCAGAGGAA GAGTTGCAGA AGGCTTTAGA GCTCAATGGC 
AAGAAGTTAT TGGGTCAGCC AGTCAAACTG GACAAAGCCA GAAGCAAAGA GAACTCGCAG 
GAAAACAAGA AAGAGAGAGA CGCCCGCACT TTATTCGTGA AGAATCTTCC ATACTCAATA 
ACACAAGACG ACCTGAGGGA AATCTTTGAC CAGGCTGTAG ATATCAGAGT ACCGATGGGC 
AACACCGGCA CTAGCAGAGG AATCGCCTAC ATTGAGTTCA AGACCGAGGC CATTGCAGAG 
AAGGCTTTAG AGGAGGCGCA GGGCTCAGAT GTTCAGGGAC GCTCCATCAT CGTCGACTTC 
ACAGGAGACA AGAGTCGGCA GGGCGGCAGA GGTGCTCCGT CTGCAAGTAA AGTGCTGGTG 
GTGAACAACC TGGCGTTCAG TGCGAGTGAA GACTCCCTCC AGAGTGTGTT CGAGAAGGCC 
GTGTCCATCA GAATACCACA GAACAACGGC AGACCCAAAG GATATGCGTT TGTGGAGTTT 
GAGAATGTGG AGGACTCCAA GGAGGCGCTG GAGAACTGCA ACAACACAGA TATCGAGGGC 
AGGAGTATTA GATTAGAGTA CAGCCAGAAC GACAGAGAAA GAGGCGGCGG AGGAAGAGGA 
AACTCTGGAC CTACGAAAAC CCTGTTTGTC AAAGGCCTGT CAGACGACAC CACAGATCAG 
ACTCTGAAAG ACTCTTTTGA TGGAGCCATC GCAGCCAGAA TCGCCACAGA CAGAGACACC 
GGATCATCGA AAGGGTTTGG TTTTGTAGAC TTCGATAACG AGCAAGACTG CAAGGCAGCT 
AAAGAAGCCA TGGATGACGG AGAGATCGAC GGAAACAAAG TGACTCTGGA CTACGCCAAG 
CCCAAAGGAG AAGGTGGACG AGGAGGATTC GGCGGAGGAA GAGGAGGCTT TGGAGGACGA 
GGCGGCGGAA GGGGTGGATT TGGAGGTAGA GGAGGAGGAG GACGAGGTGG CGGATTCAGG 
GGAGGACGAG GAGGAAGAGG CGGCGGCGGA GGATTCAGAG GAGGAAGAGG AGGAGGAGGC 
CGAGGTGGAT TCGGGGACAG ACCACAAGGA AAGAAGATCA AGTTTGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170605.1
CDS	136..2109
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_005170548.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170548.4

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGAAT CTGAAGAGGA AGAAGCTCCG CCTCCTAAAA AAGCTGCAGC AGCGGCTAAA 
CCTGCAGCTA AAGCACCTGC AGCCAAAGAG GAGTCAGATG AAGAGGAGGA TGATGACGAA 
GAGGAGGATG ATTCAGAAGA GGAGGAAATG GACACAACAC CTGCGCCCAA AGCCAAGAAA 
GCAGGAATGG TGAAGGCCAA GGAGGAGTCT GAGGAAGAAG ATGACGACGA TGAGGAAGAT 
GATGATGATG AGGAGGAAGA AGAAACCCCG GCCACTCCAG CTAAGAGGAA AGCCGAGACC 
AAAAAAGAGA AGGGAACGCC GCCGGCTAAG AAGGCTAAAA CCGATGGCGA GGGTTTCAGT 
CTTTTTTTGG GCAACCTGAA CAACAATAAA GACTTTGACG AACTGAAGTC GGCTATCTCA 
AAGTTCTTCT CAAAGGAGGG CCTTGAAATT CAGGATGTGC GACTTGGTGG AACAAAGAAA 
TTCGGCTACG TTGACTTCGC CTCAGAGGAA GAGTTGCAGA AGGCTTTAGA GCTCAATGGC 
AAGAAGTTAT TGGGTCAGCC AGTCAAACTG GACAAAGCCA GAAGCAAAGA GAACTCGCAG 
GAAAACAAGA AAGAGAGAGA CGCCCGCACT TTATTCGTGA AGAATCTTCC ATACTCAATA 
ACACAAGACG ACCTGAGGGA AATCTTTGAC CAGGCTGTAG ATATCAGAGT ACCGATGGGC 
AACACCGGCA CTAGCAGAGG AATCGCCTAC ATTGAGTTCA AGACCGAGGC CATTGCAGAG 
AAGGCTTTAG AGGAGGCGCA GGGCTCAGAT GTTCAGGGAC GCTCCATCAT CGTCGACTTC 
ACAGGAGACA AGAGTCGGCA GGGCGGCAGA GGTGCTCCGT CTGCAAGTAA AGTGCTGGTG 
GTGAACAACC TGGCGTTCAG TGCGAGTGAA GACTCCCTCC AGAGTGTGTT CGAGAAGGCC 
GTGTCCATCA GAATACCACA GAACAACGGC AGACCCAAAG GATATGCGTT TGTGGAGTTT 
GAGAATGTGG AGGACTCCAA GGAGGCGCTG GAGAACTGCA ACAACACAGA TATCGAGGGC 
AGGAGTATTA GATTAGAGTA CAGCCAGAAC GACAGAGAAA GAGGCGGCGG AGGAAGAGGA 
AACTCTGGAC CTACGAAAAC CCTGTTTGTC AAAGGCCTGT CAGACGACAC CACAGATCAG 
ACTCTGAAAG ACTCTTTTGA TGGAGCCATC GCAGCCAGAA TCGCCACAGA CAGAGACACC 
GGATCATCGA AAGGGTTTGG TTTTGTAGAC TTCGATAACG AGCAAGACTG CAAGGCAGCT 
AAAGAAGCCA TGGATGACGG AGAGATCGAC GGAAACAAAG TGACTCTGGA CTACGCCAAG 
CCCAAAGGAG AAGGTGGACG AGGAGGATTC GGCGGAGGAA GAGGAGGCTT TGGAGGACGA 
GGCGGCGGAA GGGGTGGATT TGGAGGTAGA GGAGGAGGAG GACGAGGTGG CGGATTCAGG 
GGAGGACGAG GAGGAAGAGG CGGCGGCGGA GGATTCAGAG GAGGAAGAGG AGGAGGAGGC 
CGAGGTGGAT TCGGGGACAG ACCACAAGGA AAGAAGATCA AGTTTGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_005170548.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170605.1 Danio rerio nucleolin (ncl), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa27216
Clone ID Related Accession (Same CDS sequence)	XM_005170548.4 , XM_005170548.3
Accession Version	XM_005170548.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170548.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGAAT CTGAAGAGGA AGAAGCTCCG CCTCCTAAAA AAGCTGCAGC AGCGGCTAAA 
CCTGCAGCTA AAGCACCTGC AGCCAAAGAG GAGTCAGATG AAGAGGAGGA TGATGACGAA 
GAGGAGGATG ATTCAGAAGA GGAGGAAATG GACACAACAC CTGCGCCCAA AGCCAAGAAA 
GCAGGAATGG TGAAGGCCAA GGAGGAGTCT GAGGAAGAAG ATGACGACGA TGAGGAAGAT 
GATGATGATG AGGAGGAAGA AGAAACCCCG GCCACTCCAG CTAAGAGGAA AGCCGAGACC 
AAAAAAGAGA AGGGAACGCC GCCGGCTAAG AAGGCTAAAA CCGATGGCGA GGGTTTCAGT 
CTTTTTTTGG GCAACCTGAA CAACAATAAA GACTTTGACG AACTGAAGTC GGCTATCTCA 
AAGTTCTTCT CAAAGGAGGG CCTTGAAATT CAGGATGTGC GACTTGGTGG AACAAAGAAA 
TTCGGCTACG TTGACTTCGC CTCAGAGGAA GAGTTGCAGA AGGCTTTAGA GCTCAATGGC 
AAGAAGTTAT TGGGTCAGCC AGTCAAACTG GACAAAGCCA GAAGCAAAGA GAACTCGCAG 
GAAAACAAGA AAGAGAGAGA CGCCCGCACT TTATTCGTGA AGAATCTTCC ATACTCAATA 
ACACAAGACG ACCTGAGGGA AATCTTTGAC CAGGCTGTAG ATATCAGAGT ACCGATGGGC 
AACACCGGCA CTAGCAGAGG AATCGCCTAC ATTGAGTTCA AGACCGAGGC CATTGCAGAG 
AAGGCTTTAG AGGAGGCGCA GGGCTCAGAT GTTCAGGGAC GCTCCATCAT CGTCGACTTC 
ACAGGAGACA AGAGTCGGCA GGGCGGCAGA GGTGCTCCGT CTGCAAGTAA AGTGCTGGTG 
GTGAACAACC TGGCGTTCAG TGCGAGTGAA GACTCCCTCC AGAGTGTGTT CGAGAAGGCC 
GTGTCCATCA GAATACCACA GAACAACGGC AGACCCAAAG GATATGCGTT TGTGGAGTTT 
GAGAATGTGG AGGACTCCAA GGAGGCGCTG GAGAACTGCA ACAACACAGA TATCGAGGGC 
AGGAGTATTA GATTAGAGTA CAGCCAGAAC GACAGAGAAA GAGGCGGCGG AGGAAGAGGA 
AACTCTGGAC CTACGAAAAC CCTGTTTGTC AAAGGCCTGT CAGACGACAC CACAGATCAG 
ACTCTGAAAG ACTCTTTTGA TGGAGCCATC GCAGCCAGAA TCGCCACAGA CAGAGACACC 
GGATCATCGA AAGGGTTTGG TTTTGTAGAC TTCGATAACG AGCAAGACTG CAAGGCAGCT 
AAAGAAGCCA TGGATGACGG AGAGATCGAC GGAAACAAAG TGACTCTGGA CTACGCCAAG 
CCCAAAGGAG AAGGTGGACG AGGAGGATTC GGCGGAGGAA GAGGAGGCTT TGGAGGACGA 
GGCGGCGGAA GGGGTGGATT TGGAGGTAGA GGAGGAGGAG GACGAGGTGG CGGATTCAGG 
GGAGGACGAG GAGGAAGAGG CGGCGGCGGA GGATTCAGAG GAGGAAGAGG AGGAGGAGGC 
CGAGGTGGAT TCGGGGACAG ACCACAAGGA AAGAAGATCA AGTTTGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170605.1
CDS	130..2103
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_005170548.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170548.3

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGGCACCAC CAGTCAAAGC TGTAGCGAAG AAAAACGCGG CTCCTGTGAA GGCAGTGAAA 
AACGGAAAAG CTGCTGCCAA ACAGGAGAGT GAGGATGAAG ATGACGACGA TGAAGATGAT 
GAATCTGAAG AGGAAGCTCC GCCTCCAAAG AAGGCCGCTA AAGCTCCAGC TAAAGCAGCA 
GCTCCTGCAA AGAAAGCAGC TCCTGCTGAA GAGTCCAGTG AAGAGGATGA CGACGATGAT 
GATGATGAAT CTGAAGAGGA AGAAGCTCCG CCTCCTAAAA AAGCTGCAGC AGCGGCTAAA 
CCTGCAGCTA AAGCACCTGC AGCCAAAGAG GAGTCAGATG AAGAGGAGGA TGATGACGAA 
GAGGAGGATG ATTCAGAAGA GGAGGAAATG GACACAACAC CTGCGCCCAA AGCCAAGAAA 
GCAGGAATGG TGAAGGCCAA GGAGGAGTCT GAGGAAGAAG ATGACGACGA TGAGGAAGAT 
GATGATGATG AGGAGGAAGA AGAAACCCCG GCCACTCCAG CTAAGAGGAA AGCCGAGACC 
AAAAAAGAGA AGGGAACGCC GCCGGCTAAG AAGGCTAAAA CCGATGGCGA GGGTTTCAGT 
CTTTTTTTGG GCAACCTGAA CAACAATAAA GACTTTGACG AACTGAAGTC GGCTATCTCA 
AAGTTCTTCT CAAAGGAGGG CCTTGAAATT CAGGATGTGC GACTTGGTGG AACAAAGAAA 
TTCGGCTACG TTGACTTCGC CTCAGAGGAA GAGTTGCAGA AGGCTTTAGA GCTCAATGGC 
AAGAAGTTAT TGGGTCAGCC AGTCAAACTG GACAAAGCCA GAAGCAAAGA GAACTCGCAG 
GAAAACAAGA AAGAGAGAGA CGCCCGCACT TTATTCGTGA AGAATCTTCC ATACTCAATA 
ACACAAGACG ACCTGAGGGA AATCTTTGAC CAGGCTGTAG ATATCAGAGT ACCGATGGGC 
AACACCGGCA CTAGCAGAGG AATCGCCTAC ATTGAGTTCA AGACCGAGGC CATTGCAGAG 
AAGGCTTTAG AGGAGGCGCA GGGCTCAGAT GTTCAGGGAC GCTCCATCAT CGTCGACTTC 
ACAGGAGACA AGAGTCGGCA GGGCGGCAGA GGTGCTCCGT CTGCAAGTAA AGTGCTGGTG 
GTGAACAACC TGGCGTTCAG TGCGAGTGAA GACTCCCTCC AGAGTGTGTT CGAGAAGGCC 
GTGTCCATCA GAATACCACA GAACAACGGC AGACCCAAAG GATATGCGTT TGTGGAGTTT 
GAGAATGTGG AGGACTCCAA GGAGGCGCTG GAGAACTGCA ACAACACAGA TATCGAGGGC 
AGGAGTATTA GATTAGAGTA CAGCCAGAAC GACAGAGAAA GAGGCGGCGG AGGAAGAGGA 
AACTCTGGAC CTACGAAAAC CCTGTTTGTC AAAGGCCTGT CAGACGACAC CACAGATCAG 
ACTCTGAAAG ACTCTTTTGA TGGAGCCATC GCAGCCAGAA TCGCCACAGA CAGAGACACC 
GGATCATCGA AAGGGTTTGG TTTTGTAGAC TTCGATAACG AGCAAGACTG CAAGGCAGCT 
AAAGAAGCCA TGGATGACGG AGAGATCGAC GGAAACAAAG TGACTCTGGA CTACGCCAAG 
CCCAAAGGAG AAGGTGGACG AGGAGGATTC GGCGGAGGAA GAGGAGGCTT TGGAGGACGA 
GGCGGCGGAA GGGGTGGATT TGGAGGTAGA GGAGGAGGAG GACGAGGTGG CGGATTCAGG 
GGAGGACGAG GAGGAAGAGG CGGCGGCGGA GGATTCAGAG GAGGAAGAGG AGGAGGAGGC 
CGAGGTGGAT TCGGGGACAG ACCACAAGGA AAGAAGATCA AGTTTGATGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_009296513.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294788.1 Danio rerio nucleolin (ncl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46518
Clone ID Related Accession (Same CDS sequence)	XM_009296513.2
Accession Version	XM_009296513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG ATGAATCCGA GGAAGAGGCT CCACCTCCCA AGAAGGCTCC TGCCAAAGCT 
ACACCAGCTA AAGCCACACC AGCCAAGAAA GCAGCTCCCG TGAAGAAAGC AGCTCCTGCT 
GAGGAGTCTG AGGATGACGA TGATGATGAT GAATCTGAAG AGGAAGAAGC TCCGCCTCCT 
AAAAAAGCTG CAGCAGCGGC TAAACCTGCA GCTAAAGCAC CTGCAGCCAA AGAGGAGTCA 
GATGAAGAGG AGGATGATGA CGAAGAGGAG GATGATTCAG AAGAGGAGGA AATGGACACA 
ACACCTGCGC CCAAAGCCAA GAAAGCAGGA ATGGTGAAGG CCAAGGAGGA GTCTGAGGAA 
GAAGATGACG ACGATGAGGA AGATGATGAT GATGAGGAGG AAGAAGAAAC CCCGGCCACT 
CCAGCTAAGA GGAAAGCCGA GACCAAAAAA GAGAAGGGAA CGCCGCCGGC TAAGAAGGCT 
AAAACCGATG GCGAGGGTTT CAGTCTTTTT TTGGGCAACC TGAACAACAA TAAAGACTTT 
GACGAACTGA AGTCGGCTAT CTCAAAGTTC TTCTCAAAGG AGGGCCTTGA AATTCAGGAT 
GTGCGACTTG GTGGAACAAA GAAATTCGGC TACGTTGACT TCGCCTCAGA GGAAGAGTTG 
CAGAAGGCTT TAGAGCTCAA TGGCAAGAAG TTATTGGGTC AGCCAGTCAA ACTGGACAAA 
GCCAGAAGCA AAGAGAACTC GCAGGAAAAC AAGAAAGAGA GAGACGCCCG CACTTTATTC 
GTGAAGAATC TTCCATACTC AATAACACAA GACGACCTGA GGGAAATCTT TGACCAGGCT 
GTAGATATCA GAGTACCGAT GGGCAACACC GGCACTAGCA GAGGAATCGC CTACATTGAG 
TTCAAGACCG AGGCCATTGC AGAGAAGGCT TTAGAGGAGG CGCAGGGCTC AGATGTTCAG 
GGACGCTCCA TCATCGTCGA CTTCACAGGA GACAAGAGTC GGCAGGGCGG CAGAGGTGCT 
CCGTCTGCAA GTAAAGTGCT GGTGGTGAAC AACCTGGCGT TCAGTGCGAG TGAAGACTCC 
CTCCAGAGTG TGTTCGAGAA GGCCGTGTCC ATCAGAATAC CACAGAACAA CGGCAGACCC 
AAAGGATATG CGTTTGTGGA GTTTGAGAAT GTGGAGGACT CCAAGGAGGC GCTGGAGAAC 
TGCAACAACA CAGATATCGA GGGCAGGAGT ATTAGATTAG AGTACAGCCA GAACGACAGA 
GAAAGAGGCG GCGGAGGAAG AGGAAACTCT GGACCTACGA AAACCCTGTT TGTCAAAGGC 
CTGTCAGACG ACACCACAGA TCAGACTCTG AAAGACTCTT TTGATGGAGC CATCGCAGCC 
AGAATCGCCA CAGACAGAGA CACCGGATCA TCGAAAGGGT TTGGTTTTGT AGACTTCGAT 
AACGAGCAAG ACTGCAAGGC AGCTAAAGAA GCCATGGATG ACGGAGAGAT CGACGGAAAC 
AAAGTGACTC TGGACTACGC CAAGCCCAAA GGAGAAGGTG GACGAGGAGG ATTCGGCGGA 
GGAAGAGGAG GCTTTGGAGG ACGAGGCGGC GGAAGGGGTG GATTTGGAGG TAGAGGAGGA 
GGAGGACGAG GTGGCGGATT CAGGGGAGGA CGAGGAGGAA GAGGCGGCGG CGGAGGATTC 
AGAGGAGGAA GAGGAGGAGG AGGCCGAGGT GGATTCGGGG ACAGACCACA AGGAAAGAAG 
ATCAAGTTTG ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294788.1
CDS	127..2244
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEQAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDDESEEEAPPPKKAPAKATPAKATPAKKAAPVKKAAPAEESEDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_009296513.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296513.2

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG ATGAATCCGA GGAAGAGGCT CCACCTCCCA AGAAGGCTCC TGCCAAAGCT 
ACACCAGCTA AAGCCACACC AGCCAAGAAA GCAGCTCCCG TGAAGAAAGC AGCTCCTGCT 
GAGGAGTCTG AGGATGACGA TGATGATGAT GAATCTGAAG AGGAAGAAGC TCCGCCTCCT 
AAAAAAGCTG CAGCAGCGGC TAAACCTGCA GCTAAAGCAC CTGCAGCCAA AGAGGAGTCA 
GATGAAGAGG AGGATGATGA CGAAGAGGAG GATGATTCAG AAGAGGAGGA AATGGACACA 
ACACCTGCGC CCAAAGCCAA GAAAGCAGGA ATGGTGAAGG CCAAGGAGGA GTCTGAGGAA 
GAAGATGACG ACGATGAGGA AGATGATGAT GATGAGGAGG AAGAAGAAAC CCCGGCCACT 
CCAGCTAAGA GGAAAGCCGA GACCAAAAAA GAGAAGGGAA CGCCGCCGGC TAAGAAGGCT 
AAAACCGATG GCGAGGGTTT CAGTCTTTTT TTGGGCAACC TGAACAACAA TAAAGACTTT 
GACGAACTGA AGTCGGCTAT CTCAAAGTTC TTCTCAAAGG AGGGCCTTGA AATTCAGGAT 
GTGCGACTTG GTGGAACAAA GAAATTCGGC TACGTTGACT TCGCCTCAGA GGAAGAGTTG 
CAGAAGGCTT TAGAGCTCAA TGGCAAGAAG TTATTGGGTC AGCCAGTCAA ACTGGACAAA 
GCCAGAAGCA AAGAGAACTC GCAGGAAAAC AAGAAAGAGA GAGACGCCCG CACTTTATTC 
GTGAAGAATC TTCCATACTC AATAACACAA GACGACCTGA GGGAAATCTT TGACCAGGCT 
GTAGATATCA GAGTACCGAT GGGCAACACC GGCACTAGCA GAGGAATCGC CTACATTGAG 
TTCAAGACCG AGGCCATTGC AGAGAAGGCT TTAGAGGAGG CGCAGGGCTC AGATGTTCAG 
GGACGCTCCA TCATCGTCGA CTTCACAGGA GACAAGAGTC GGCAGGGCGG CAGAGGTGCT 
CCGTCTGCAA GTAAAGTGCT GGTGGTGAAC AACCTGGCGT TCAGTGCGAG TGAAGACTCC 
CTCCAGAGTG TGTTCGAGAA GGCCGTGTCC ATCAGAATAC CACAGAACAA CGGCAGACCC 
AAAGGATATG CGTTTGTGGA GTTTGAGAAT GTGGAGGACT CCAAGGAGGC GCTGGAGAAC 
TGCAACAACA CAGATATCGA GGGCAGGAGT ATTAGATTAG AGTACAGCCA GAACGACAGA 
GAAAGAGGCG GCGGAGGAAG AGGAAACTCT GGACCTACGA AAACCCTGTT TGTCAAAGGC 
CTGTCAGACG ACACCACAGA TCAGACTCTG AAAGACTCTT TTGATGGAGC CATCGCAGCC 
AGAATCGCCA CAGACAGAGA CACCGGATCA TCGAAAGGGT TTGGTTTTGT AGACTTCGAT 
AACGAGCAAG ACTGCAAGGC AGCTAAAGAA GCCATGGATG ACGGAGAGAT CGACGGAAAC 
AAAGTGACTC TGGACTACGC CAAGCCCAAA GGAGAAGGTG GACGAGGAGG ATTCGGCGGA 
GGAAGAGGAG GCTTTGGAGG ACGAGGCGGC GGAAGGGGTG GATTTGGAGG TAGAGGAGGA 
GGAGGACGAG GTGGCGGATT CAGGGGAGGA CGAGGAGGAA GAGGCGGCGG CGGAGGATTC 
AGAGGAGGAA GAGGAGGAGG AGGCCGAGGT GGATTCGGGG ACAGACCACA AGGAAAGAAG 
ATCAAGTTTG ATGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_009296514.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294789.1 Danio rerio nucleolin (ncl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa46519
Clone ID Related Accession (Same CDS sequence)	XM_009296514.2
Accession Version	XM_009296514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG ATGAATCCGA GGAAGAGGCT CCACCTCCCA AGAAGGCTCC TGCCAAAGCT 
ACACCAGCTA AAGCCACACC AGCCAAGAAA GCAGCTCCCG TGAAGAAAGC AGCTCCTGCT 
GAGGAGTCTG AGGATGACGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294789.1
CDS	127..2241
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEQAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDDESEEEAPPPKKAPAKATPAKATPAKKAAPVKKAAPAEESEDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_009296514.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296514.2

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG ATGAATCCGA GGAAGAGGCT CCACCTCCCA AGAAGGCTCC TGCCAAAGCT 
ACACCAGCTA AAGCCACACC AGCCAAGAAA GCAGCTCCCG TGAAGAAAGC AGCTCCTGCT 
GAGGAGTCTG AGGATGACGA TGATGATGAA TCTGAAGAGG AAGAAGCTCC GCCTCCTAAA 
AAAGCTGCAG CAGCGGCTAA ACCTGCAGCT AAAGCACCTG CAGCCAAAGA GGAGTCAGAT 
GAAGAGGAGG ATGATGACGA AGAGGAGGAT GATTCAGAAG AGGAGGAAAT GGACACAACA 
CCTGCGCCCA AAGCCAAGAA AGCAGGAATG GTGAAGGCCA AGGAGGAGTC TGAGGAAGAA 
GATGACGACG ATGAGGAAGA TGATGATGAT GAGGAGGAAG AAGAAACCCC GGCCACTCCA 
GCTAAGAGGA AAGCCGAGAC CAAAAAAGAG AAGGGAACGC CGCCGGCTAA GAAGGCTAAA 
ACCGATGGCG AGGGTTTCAG TCTTTTTTTG GGCAACCTGA ACAACAATAA AGACTTTGAC 
GAACTGAAGT CGGCTATCTC AAAGTTCTTC TCAAAGGAGG GCCTTGAAAT TCAGGATGTG 
CGACTTGGTG GAACAAAGAA ATTCGGCTAC GTTGACTTCG CCTCAGAGGA AGAGTTGCAG 
AAGGCTTTAG AGCTCAATGG CAAGAAGTTA TTGGGTCAGC CAGTCAAACT GGACAAAGCC 
AGAAGCAAAG AGAACTCGCA GGAAAACAAG AAAGAGAGAG ACGCCCGCAC TTTATTCGTG 
AAGAATCTTC CATACTCAAT AACACAAGAC GACCTGAGGG AAATCTTTGA CCAGGCTGTA 
GATATCAGAG TACCGATGGG CAACACCGGC ACTAGCAGAG GAATCGCCTA CATTGAGTTC 
AAGACCGAGG CCATTGCAGA GAAGGCTTTA GAGGAGGCGC AGGGCTCAGA TGTTCAGGGA 
CGCTCCATCA TCGTCGACTT CACAGGAGAC AAGAGTCGGC AGGGCGGCAG AGGTGCTCCG 
TCTGCAAGTA AAGTGCTGGT GGTGAACAAC CTGGCGTTCA GTGCGAGTGA AGACTCCCTC 
CAGAGTGTGT TCGAGAAGGC CGTGTCCATC AGAATACCAC AGAACAACGG CAGACCCAAA 
GGATATGCGT TTGTGGAGTT TGAGAATGTG GAGGACTCCA AGGAGGCGCT GGAGAACTGC 
AACAACACAG ATATCGAGGG CAGGAGTATT AGATTAGAGT ACAGCCAGAA CGACAGAGAA 
AGAGGCGGCG GAGGAAGAGG AAACTCTGGA CCTACGAAAA CCCTGTTTGT CAAAGGCCTG 
TCAGACGACA CCACAGATCA GACTCTGAAA GACTCTTTTG ATGGAGCCAT CGCAGCCAGA 
ATCGCCACAG ACAGAGACAC CGGATCATCG AAAGGGTTTG GTTTTGTAGA CTTCGATAAC 
GAGCAAGACT GCAAGGCAGC TAAAGAAGCC ATGGATGACG GAGAGATCGA CGGAAACAAA 
GTGACTCTGG ACTACGCCAA GCCCAAAGGA GAAGGTGGAC GAGGAGGATT CGGCGGAGGA 
AGAGGAGGCT TTGGAGGACG AGGCGGCGGA AGGGGTGGAT TTGGAGGTAG AGGAGGAGGA 
GGACGAGGTG GCGGATTCAG GGGAGGACGA GGAGGAAGAG GCGGCGGCGG AGGATTCAGA 
GGAGGAAGAG GAGGAGGAGG CCGAGGTGGA TTCGGGGACA GACCACAAGG AAAGAAGATC 
AAGTTTGATG ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ncl ( XM_009296515.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294790.1 Danio rerio nucleolin (ncl), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa46520
Clone ID Related Accession (Same CDS sequence)	XM_009296515.2
Accession Version	XM_009296515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG AATCTGAAGA GGAAGAAGCT CCGCCTCCTA AAAAAGCTGC AGCAGCGGCT 
AAACCTGCAG CTAAAGCACC TGCAGCCAAA GAGGAGTCAG ATGAAGAGGA GGATGATGAC 
GAAGAGGAGG ATGATTCAGA AGAGGAGGAA ATGGACACAA CACCTGCGCC CAAAGCCAAG 
AAAGCAGGAA TGGTGAAGGC CAAGGAGGAG TCTGAGGAAG AAGATGACGA CGATGAGGAA 
GATGATGATG ATGAGGAGGA AGAAGAAACC CCGGCCACTC CAGCTAAGAG GAAAGCCGAG 
ACCAAAAAAG AGAAGGGAAC GCCGCCGGCT AAGAAGGCTA AAACCGATGG CGAGGGTTTC 
AGTCTTTTTT TGGGCAACCT GAACAACAAT AAAGACTTTG ACGAACTGAA GTCGGCTATC 
TCAAAGTTCT TCTCAAAGGA GGGCCTTGAA ATTCAGGATG TGCGACTTGG TGGAACAAAG 
AAATTCGGCT ACGTTGACTT CGCCTCAGAG GAAGAGTTGC AGAAGGCTTT AGAGCTCAAT 
GGCAAGAAGT TATTGGGTCA GCCAGTCAAA CTGGACAAAG CCAGAAGCAA AGAGAACTCG 
CAGGAAAACA AGAAAGAGAG AGACGCCCGC ACTTTATTCG TGAAGAATCT TCCATACTCA 
ATAACACAAG ACGACCTGAG GGAAATCTTT GACCAGGCTG TAGATATCAG AGTACCGATG 
GGCAACACCG GCACTAGCAG AGGAATCGCC TACATTGAGT TCAAGACCGA GGCCATTGCA 
GAGAAGGCTT TAGAGGAGGC GCAGGGCTCA GATGTTCAGG GACGCTCCAT CATCGTCGAC 
TTCACAGGAG ACAAGAGTCG GCAGGGCGGC AGAGGTGCTC CGTCTGCAAG TAAAGTGCTG 
GTGGTGAACA ACCTGGCGTT CAGTGCGAGT GAAGACTCCC TCCAGAGTGT GTTCGAGAAG 
GCCGTGTCCA TCAGAATACC ACAGAACAAC GGCAGACCCA AAGGATATGC GTTTGTGGAG 
TTTGAGAATG TGGAGGACTC CAAGGAGGCG CTGGAGAACT GCAACAACAC AGATATCGAG 
GGCAGGAGTA TTAGATTAGA GTACAGCCAG AACGACAGAG AAAGAGGCGG CGGAGGAAGA 
GGAAACTCTG GACCTACGAA AACCCTGTTT GTCAAAGGCC TGTCAGACGA CACCACAGAT 
CAGACTCTGA AAGACTCTTT TGATGGAGCC ATCGCAGCCA GAATCGCCAC AGACAGAGAC 
ACCGGATCAT CGAAAGGGTT TGGTTTTGTA GACTTCGATA ACGAGCAAGA CTGCAAGGCA 
GCTAAAGAAG CCATGGATGA CGGAGAGATC GACGGAAACA AAGTGACTCT GGACTACGCC 
AAGCCCAAAG GAGAAGGTGG ACGAGGAGGA TTCGGCGGAG GAAGAGGAGG CTTTGGAGGA 
CGAGGCGGCG GAAGGGGTGG ATTTGGAGGT AGAGGAGGAG GAGGACGAGG TGGCGGATTC 
AGGGGAGGAC GAGGAGGAAG AGGCGGCGGC GGAGGATTCA GAGGAGGAAG AGGAGGAGGA 
GGCCGAGGTG GATTCGGGGA CAGACCACAA GGAAAGAAGA TCAAGTTTGA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294790.1
CDS	127..2103
Translation	MVKLAKAAKKDAPPKKKAPPPPKEVEEESSEEDSDDDEEEEQAPPVKAVAKKNAAPVKAVKNGKAAAKQESEDEDDDDEDDESEEEAPPPKKAAKAPAKAAAPAKKAAPAEESSEEDDDDDDDESEEEEAPPPKKAAAAAKPAAKAPAAKEESDEEEDDDEEEDDSEEEEMDTTPAPKAKKAGMVKAKEESEEEDDDDEEDDDDEEEEETPATPAKRKAETKKEKGTPPAKKAKTDGEGFSLFLGNLNNNKDFDELKSAISKFFSKEGLEIQDVRLGGTKKFGYVDFASEEELQKALELNGKKLLGQPVKLDKARSKENSQENKKERDARTLFVKNLPYSITQDDLREIFDQAVDIRVPMGNTGTSRGIAYIEFKTEAIAEKALEEAQGSDVQGRSIIVDFTGDKSRQGGRGAPSASKVLVVNNLAFSASEDSLQSVFEKAVSIRIPQNNGRPKGYAFVEFENVEDSKEALENCNNTDIEGRSIRLEYSQNDRERGGGGRGNSGPTKTLFVKGLSDDTTDQTLKDSFDGAIAARIATDRDTGSSKGFGFVDFDNEQDCKAAKEAMDDGEIDGNKVTLDYAKPKGEGGRGGFGGGRGGFGGRGGGRGGFGGRGGGGRGGGFRGGRGGRGGGGGFRGGRGGGGRGGFGDRPQGKKIKFDD

Target ORF information:

RefSeq Version	XM_009296515.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolin (ncl), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296515.2

ORF Insert Sequence:

ATGGTAAAGC TCGCTAAGGC AGCTAAAAAA GATGCGCCGC CGAAGAAAAA GGCACCTCCA 
CCTCCTAAAG AAGTGGAAGA GGAATCCAGC GAGGAGGACA GTGATGACGA TGAGGAAGAA 
GAGCAGGCAC CACCAGTCAA AGCTGTAGCG AAGAAAAACG CGGCTCCTGT GAAGGCAGTG 
AAAAACGGAA AAGCTGCTGC CAAACAGGAG AGTGAGGATG AAGATGACGA CGATGAAGAT 
GATGAATCTG AAGAGGAAGC TCCGCCTCCA AAGAAGGCCG CTAAAGCTCC AGCTAAAGCA 
GCAGCTCCTG CAAAGAAAGC AGCTCCTGCT GAAGAGTCCA GTGAAGAGGA TGACGACGAT 
GATGATGATG AATCTGAAGA GGAAGAAGCT CCGCCTCCTA AAAAAGCTGC AGCAGCGGCT 
AAACCTGCAG CTAAAGCACC TGCAGCCAAA GAGGAGTCAG ATGAAGAGGA GGATGATGAC 
GAAGAGGAGG ATGATTCAGA AGAGGAGGAA ATGGACACAA CACCTGCGCC CAAAGCCAAG 
AAAGCAGGAA TGGTGAAGGC CAAGGAGGAG TCTGAGGAAG AAGATGACGA CGATGAGGAA 
GATGATGATG ATGAGGAGGA AGAAGAAACC CCGGCCACTC CAGCTAAGAG GAAAGCCGAG 
ACCAAAAAAG AGAAGGGAAC GCCGCCGGCT AAGAAGGCTA AAACCGATGG CGAGGGTTTC 
AGTCTTTTTT TGGGCAACCT GAACAACAAT AAAGACTTTG ACGAACTGAA GTCGGCTATC 
TCAAAGTTCT TCTCAAAGGA GGGCCTTGAA ATTCAGGATG TGCGACTTGG TGGAACAAAG 
AAATTCGGCT ACGTTGACTT CGCCTCAGAG GAAGAGTTGC AGAAGGCTTT AGAGCTCAAT 
GGCAAGAAGT TATTGGGTCA GCCAGTCAAA CTGGACAAAG CCAGAAGCAA AGAGAACTCG 
CAGGAAAACA AGAAAGAGAG AGACGCCCGC ACTTTATTCG TGAAGAATCT TCCATACTCA 
ATAACACAAG ACGACCTGAG GGAAATCTTT GACCAGGCTG TAGATATCAG AGTACCGATG 
GGCAACACCG GCACTAGCAG AGGAATCGCC TACATTGAGT TCAAGACCGA GGCCATTGCA 
GAGAAGGCTT TAGAGGAGGC GCAGGGCTCA GATGTTCAGG GACGCTCCAT CATCGTCGAC 
TTCACAGGAG ACAAGAGTCG GCAGGGCGGC AGAGGTGCTC CGTCTGCAAG TAAAGTGCTG 
GTGGTGAACA ACCTGGCGTT CAGTGCGAGT GAAGACTCCC TCCAGAGTGT GTTCGAGAAG 
GCCGTGTCCA TCAGAATACC ACAGAACAAC GGCAGACCCA AAGGATATGC GTTTGTGGAG 
TTTGAGAATG TGGAGGACTC CAAGGAGGCG CTGGAGAACT GCAACAACAC AGATATCGAG 
GGCAGGAGTA TTAGATTAGA GTACAGCCAG AACGACAGAG AAAGAGGCGG CGGAGGAAGA 
GGAAACTCTG GACCTACGAA AACCCTGTTT GTCAAAGGCC TGTCAGACGA CACCACAGAT 
CAGACTCTGA AAGACTCTTT TGATGGAGCC ATCGCAGCCA GAATCGCCAC AGACAGAGAC 
ACCGGATCAT CGAAAGGGTT TGGTTTTGTA GACTTCGATA ACGAGCAAGA CTGCAAGGCA 
GCTAAAGAAG CCATGGATGA CGGAGAGATC GACGGAAACA AAGTGACTCT GGACTACGCC 
AAGCCCAAAG GAGAAGGTGG ACGAGGAGGA TTCGGCGGAG GAAGAGGAGG CTTTGGAGGA 
CGAGGCGGCG GAAGGGGTGG ATTTGGAGGT AGAGGAGGAG GAGGACGAGG TGGCGGATTC 
AGGGGAGGAC GAGGAGGAAG AGGCGGCGGC GGAGGATTCA GAGGAGGAAG AGGAGGAGGA 
GGCCGAGGTG GATTCGGGGA CAGACCACAA GGAAAGAAGA TCAAGTTTGA TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytoplasmic polyadenylation-mediated translational control of maternal mRNAs directs maternal-to-zygotic transition.
Development (Cambridge, England)145(1)(2018 Jan)
Winata CL,?api?ski M,Pryszcz L,Vaz C,Bin Ismail MH,Nama S,Hajan HS,Lee SGP,Korzh V,Sampath P,Tanavde V,Mathavan S

Cross talk between TP53 and c-Myc in the pathophysiology of Diamond-Blackfan anemia: Evidence from RPL11-deficient in?vivo and in?vitro models.
Biochemical and biophysical research communications495(2)1839-1845(2018 Jan)
Chakraborty A,Uechi T,Nakajima Y,Gazda HT,O'Donohue MF,Gleizes PE,Kenmochi N

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Systems proteomics of cardiac chromatin identifies nucleolin as a regulator of growth and cellular plasticity in cardiomyocytes.
American journal of physiology. Heart and circulatory physiology305(11)H1624-38(2013 Dec)
Monte E,Mouillesseaux K,Chen H,Kimball T,Ren S,Wang Y,Chen JN,Vondriska TM,Franklin S

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ncl cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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