epha4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	epha4b
Entrez Gene ID	64270
Full Name	eph receptor A4b
Synonyms	EphA3,epha4,id:ibd2782,rtk2
General protein information	Preferred Names eph receptor A4b Names eph receptor A4b eph-like receptor tyrosine kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298720.2	XP_009296995.1	eph receptor A4b isoform X1
NM_153658.1	NP_705944.1	eph receptor A4b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ek1	NP_571170.1
Danio rerio (zebrafish)	epha4b	NP_705944.1
Xenopus tropicalis (tropical clawed frog)	LOC100494929	XP_004917824.1

Gene Ontology
Bibliography

Related articles in PubMed

Ephrin-A1-EphA4 signaling negatively regulates myelination in the central nervous system.
Harboe M, Torvund-Jensen J, Kjaer-Sorensen K, Laursen LS
Glia(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Bouwmeester MC, Ruiter S, Lommelaars T, Sippel J, Hodemaekers HM, van den Brandhof EJ, Pennings JL, Kamstra JH, Jelinek J, Issa JP, Legler J, van der Ven LT
Toxicology and applied pharmacology29184-96(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EPHA6, a gene implicated in retinal axon guidance, is one of HMX1 targets in eye development.
Title: A dimerized HMX1 inhibits EPHA6/epha4b in mouse and zebrafish retinas.

The following epha4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the epha4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa39412	XM_009298720.2 Latest version!	Danio rerio eph receptor A4b (epha4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa00442	NM_153658.1 Latest version!	Danio rerio eph receptor A4b (epha4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

epha4b ( XM_009298720.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296995.1 Danio rerio eph receptor A4b (epha4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39412
Clone ID Related Accession (Same CDS sequence)	XM_009298720.2
Accession Version	XM_009298720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eph receptor A4b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACCC TTTGGATTTA TCCGTCACTT CTTTTCTTTC TCTTTCCCTC CCTCACACCG 
AGTCAAGCCT TACGGAATTA CCCAGAGAAT GAAGTTACAT TGCTGGACAC AATGTCTGCT 
CTGGGAGACC TTGGTTGGGA GGCCTATCCT GCTGAAGGGT GGGAGGAAAT CAGTGTGATG 
GATGAGAGAA ACACCCCCAT GCGTTCCTAC CAGGTTTGCA ACGTGATGGA GGCCAGTCAG 
AATAACTGGC TTCGCACTCG GCACATTGCC CGGGAAGGAG CCCAGCGGGT CTACATCGAG 
ATCAAGTTCA CCCTGCGTGA CTGCAACAGC CTGCCAGGAG TCCCCGGCAC CTGCAAAGAA 
ACCTTCAACA TGTACTACTA TGAGTCCAAC AACCCCAACC TGTGGTTCAT CAAGGAGAGC 
CAGTACATAA AGATTGACAC CATCGCCGCT GACGAGAGCT TCACACAGAC CGACGTCGGT 
GATCGGGTCA TGAAACTAAA CACGGAAATC CGGGACATCA ATAACTTGAG CAAGAAGGGT 
TTTTATCTGG CTTTCCAAGA TGTCGGAGCC TGCATCGCGC TGGTGTCTCT GAGGGTCTTT 
TATAAGAAGT GTCCTCTGGC GGTGTTGAAC TTGGCCCAGT TTCCCGATAC GGTGACTGGA 
GGAGATTCCG CTTTGGTGGA GGTTAGAGGG TCTTGTGTGA ATGATTCGGA GGAGTTTGAG 
GCTCCGAGGA TGTACTGCAG TGGAGATGGT GGATGGCTGG TGCCCATCGG ACGCTGCGTC 
TGCAAGCCGG GCTTCGAGGA GAATAAGGAC TACTGTCAGG CCTGCAGGCC AGGCACCTAC 
AAAGCCTCTT CCATGGACGC CTACTGCTCC AAATGTCCTC CACACAGTTT CTCTCACCAG 
GAAAAGGCTT CTGAATGTTC CTGTGAGAAA GGATTTTACA GAGCTGACAT GGACCCGCGG 
TCAATGGCCT GCACCCGGCC TCCGTCAGCA CCGGAGAACC CGATCTCCAC CCTGAACGAC 
ACCTCAGTGA CTTTAGAATG GAGTCCTCCT CGAGACAGCG GCGGACGAGG AGATGTGACC 
TACAGCATAC ACTGCAGAAA GTGTGCTGGC GACGGAAGGA TATGCAGCCC CTGCAGCAAC 
AATGTCCACT TTGTCCCAAG ACAGTTCGGC TTGTCGATCC CAAAAGTGCT GATCACAGAC 
CTGCTCTCCA ACACCAACTA CACCTTCAGC GTTGAAGCGG TGAATGGGGT GTCAGATCTG 
AGCCCAACCC CTAAACAACT AGTCACAGTC AACATTACCA CCAGTCAGAC AGTGAATGTG 
ATCCTGAAGG AGAGAAAGAG TAAAGACAGT ATTACCCTGG CATGGCAGGG GCCCGACAGA 
CCAAACGGAG TCATAGTGGA ATATGAAGTC ATCTACTATG AGAAGAACCA AAGAGACCAG 
AACTACACAG TTCTGAAGAC CAAAGGGCAC ATGATGACTG TGGAGGGCCT GAAACCAGGA 
ACCACATACG TGTTCAGAGT GAGGGCTCGC ACTGATGGAG GATACGGCAA CTATGGTGGA 
GAGATCGAGC TGGAGACCAG TCATGAGGAT ATGCTGGCCA TTGGGGACCC GAACCAGTCC 
ACAATCCTAG CTGTGTCCAT AGCTGGAGGC ATTGTGCTGC TTATCTTCCT GGTTGCGTGT 
TTTGTAGTGA GCGGACGACA TTGTGGCTAC ATTAAAGCAA AGCAAGATCC AGATGAAGAA 
AAAATGCAGT TTCAGAATGG CAGAGTGAAG CTGCCAGGAA GCAGGACGTA CATTCACCCG 
CACACTTATG AGGACCCAAA TCAAGCCGTT CGGGATTTTG CTAAGGAAAT TGATGCCTCC 
ACTATACGTA TCGAGAGAGT TATAGGGGCA GGTGAGTTTG GTGAGGTGTG CAGTGGCCGT 
CTGAAGCTCC CCAGCAAGAG GGAAATCTAT GTGGCTATCA AGAGTCTGAA AGCAGGATAT 
TCAGAGAAAC AGAGGCGGGA CTTTCTGAGC GAGGCCAGCA TCATGGGCCA GTTCGACCAT 
CCCAACATCA TCCGACTGGA GGGAGTCGTG ACCAGATGCA AGCCCGTGAT GATTATTACA 
GAGTACATGG AGAATGGATC GTTGGATACT TTCCTGAAGA AGCATGATGG GCAGTTCACA 
GTCATTCAGC TGGTGGGCAT GATGCGTGGC ATCGCTTCAG GAATGAAATA TCTGTCTGAT 
ATGAGCTACG TCCACCGTGA TCTGGCAGCT CGCAACATCT TGGTCAACAG TAACCTGGTG 
TGCAAAGTGT CTGACTTTGG CCTGTCCAGA GTGCTGGAGG ATGATCCTGA AGCTGCGTAT 
ACAACACGGG GTGGAAAAAT CCCCATTCGA TGGACAGCCC CAGAGGCCAT CGCGTACAGA 
AAGTTCACCT CAGCGAGTGA CGTGTGGAGT TACGGCATCG TCATGTGGGA GGTGATTTCA 
TACGGAGAAC GGCCATATTG GGAGATGTCT AATCAAGACG TGATTAAAGC TATAGATGAG 
GGTTACAGGC TTCCCGCCCC AATGGACTGC CCCGTGGTCC TTCATCAGCT CATGCTGGAC 
TGCTGGGAGA AAAGCAGAAG TGAACGGCCC AAATTCGGGC AGATCGTCAA CACTCTTGAC 
AAACTTATCC GCAACCCCAA CAGTCTGAAG GAGCTCGCTA ACAGCTCAGT TTGGGAAGAC 
CCCACCACCC CAGAGTTCAG TGTGAGTACG GTGGATGAAT GGCTGGACGC CATCAATATG 
GGCCAGTACA AGGACAACTT TGCCAATGCG GGTTATGTTT CCCTAGATTC CATTCTGTAC 
ATCAGCATGT GTGAGTTGAG CAAGATGGAC GTGACTCTGG TGGGCCACCA AAAGAAGATT 
CTCTCCGCCG TCCAGAGTTT GCACGCACAG GGGACACACG TGCAGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296995.1
CDS	99..3029
Translation	MATLWIYPSLLFFLFPSLTPSQALRNYPENEVTLLDTMSALGDLGWEAYPAEGWEEISVMDERNTPMRSYQVCNVMEASQNNWLRTRHIAREGAQRVYIEIKFTLRDCNSLPGVPGTCKETFNMYYYESNNPNLWFIKESQYIKIDTIAADESFTQTDVGDRVMKLNTEIRDINNLSKKGFYLAFQDVGACIALVSLRVFYKKCPLAVLNLAQFPDTVTGGDSALVEVRGSCVNDSEEFEAPRMYCSGDGGWLVPIGRCVCKPGFEENKDYCQACRPGTYKASSMDAYCSKCPPHSFSHQEKASECSCEKGFYRADMDPRSMACTRPPSAPENPISTLNDTSVTLEWSPPRDSGGRGDVTYSIHCRKCAGDGRICSPCSNNVHFVPRQFGLSIPKVLITDLLSNTNYTFSVEAVNGVSDLSPTPKQLVTVNITTSQTVNVILKERKSKDSITLAWQGPDRPNGVIVEYEVIYYEKNQRDQNYTVLKTKGHMMTVEGLKPGTTYVFRVRARTDGGYGNYGGEIELETSHEDMLAIGDPNQSTILAVSIAGGIVLLIFLVACFVVSGRHCGYIKAKQDPDEEKMQFQNGRVKLPGSRTYIHPHTYEDPNQAVRDFAKEIDASTIRIERVIGAGEFGEVCSGRLKLPSKREIYVAIKSLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTRCKPVMIITEYMENGSLDTFLKKHDGQFTVIQLVGMMRGIASGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAIDEGYRLPAPMDCPVVLHQLMLDCWEKSRSERPKFGQIVNTLDKLIRNPNSLKELANSSVWEDPTTPEFSVSTVDEWLDAINMGQYKDNFANAGYVSLDSILYISMCELSKMDVTLVGHQKKILSAVQSLHAQGTHVQV

Target ORF information:

RefSeq Version	XM_009298720.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eph receptor A4b (epha4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298720.2

ORF Insert Sequence:

ATGGCAACCC TTTGGATTTA TCCGTCACTT CTTTTCTTTC TCTTTCCCTC CCTCACACCG 
AGTCAAGCCT TACGGAATTA CCCAGAGAAT GAAGTTACAT TGCTGGACAC AATGTCTGCT 
CTGGGAGACC TTGGTTGGGA GGCCTATCCT GCTGAAGGGT GGGAGGAAAT CAGTGTGATG 
GATGAGAGAA ACACCCCCAT GCGTTCCTAC CAGGTTTGCA ACGTGATGGA GGCCAGTCAG 
AATAACTGGC TTCGCACTCG GCACATTGCC CGGGAAGGAG CCCAGCGGGT CTACATCGAG 
ATCAAGTTCA CCCTGCGTGA CTGCAACAGC CTGCCAGGAG TCCCCGGCAC CTGCAAAGAA 
ACCTTCAACA TGTACTACTA TGAGTCCAAC AACCCCAACC TGTGGTTCAT CAAGGAGAGC 
CAGTACATAA AGATTGACAC CATCGCCGCT GACGAGAGCT TCACACAGAC CGACGTCGGT 
GATCGGGTCA TGAAACTAAA CACGGAAATC CGGGACATCA ATAACTTGAG CAAGAAGGGT 
TTTTATCTGG CTTTCCAAGA TGTCGGAGCC TGCATCGCGC TGGTGTCTCT GAGGGTCTTT 
TATAAGAAGT GTCCTCTGGC GGTGTTGAAC TTGGCCCAGT TTCCCGATAC GGTGACTGGA 
GGAGATTCCG CTTTGGTGGA GGTTAGAGGG TCTTGTGTGA ATGATTCGGA GGAGTTTGAG 
GCTCCGAGGA TGTACTGCAG TGGAGATGGT GGATGGCTGG TGCCCATCGG ACGCTGCGTC 
TGCAAGCCGG GCTTCGAGGA GAATAAGGAC TACTGTCAGG CCTGCAGGCC AGGCACCTAC 
AAAGCCTCTT CCATGGACGC CTACTGCTCC AAATGTCCTC CACACAGTTT CTCTCACCAG 
GAAAAGGCTT CTGAATGTTC CTGTGAGAAA GGATTTTACA GAGCTGACAT GGACCCGCGG 
TCAATGGCCT GCACCCGGCC TCCGTCAGCA CCGGAGAACC CGATCTCCAC CCTGAACGAC 
ACCTCAGTGA CTTTAGAATG GAGTCCTCCT CGAGACAGCG GCGGACGAGG AGATGTGACC 
TACAGCATAC ACTGCAGAAA GTGTGCTGGC GACGGAAGGA TATGCAGCCC CTGCAGCAAC 
AATGTCCACT TTGTCCCAAG ACAGTTCGGC TTGTCGATCC CAAAAGTGCT GATCACAGAC 
CTGCTCTCCA ACACCAACTA CACCTTCAGC GTTGAAGCGG TGAATGGGGT GTCAGATCTG 
AGCCCAACCC CTAAACAACT AGTCACAGTC AACATTACCA CCAGTCAGAC AGTGAATGTG 
ATCCTGAAGG AGAGAAAGAG TAAAGACAGT ATTACCCTGG CATGGCAGGG GCCCGACAGA 
CCAAACGGAG TCATAGTGGA ATATGAAGTC ATCTACTATG AGAAGAACCA AAGAGACCAG 
AACTACACAG TTCTGAAGAC CAAAGGGCAC ATGATGACTG TGGAGGGCCT GAAACCAGGA 
ACCACATACG TGTTCAGAGT GAGGGCTCGC ACTGATGGAG GATACGGCAA CTATGGTGGA 
GAGATCGAGC TGGAGACCAG TCATGAGGAT ATGCTGGCCA TTGGGGACCC GAACCAGTCC 
ACAATCCTAG CTGTGTCCAT AGCTGGAGGC ATTGTGCTGC TTATCTTCCT GGTTGCGTGT 
TTTGTAGTGA GCGGACGACA TTGTGGCTAC ATTAAAGCAA AGCAAGATCC AGATGAAGAA 
AAAATGCAGT TTCAGAATGG CAGAGTGAAG CTGCCAGGAA GCAGGACGTA CATTCACCCG 
CACACTTATG AGGACCCAAA TCAAGCCGTT CGGGATTTTG CTAAGGAAAT TGATGCCTCC 
ACTATACGTA TCGAGAGAGT TATAGGGGCA GGTGAGTTTG GTGAGGTGTG CAGTGGCCGT 
CTGAAGCTCC CCAGCAAGAG GGAAATCTAT GTGGCTATCA AGAGTCTGAA AGCAGGATAT 
TCAGAGAAAC AGAGGCGGGA CTTTCTGAGC GAGGCCAGCA TCATGGGCCA GTTCGACCAT 
CCCAACATCA TCCGACTGGA GGGAGTCGTG ACCAGATGCA AGCCCGTGAT GATTATTACA 
GAGTACATGG AGAATGGATC GTTGGATACT TTCCTGAAGA AGCATGATGG GCAGTTCACA 
GTCATTCAGC TGGTGGGCAT GATGCGTGGC ATCGCTTCAG GAATGAAATA TCTGTCTGAT 
ATGAGCTACG TCCACCGTGA TCTGGCAGCT CGCAACATCT TGGTCAACAG TAACCTGGTG 
TGCAAAGTGT CTGACTTTGG CCTGTCCAGA GTGCTGGAGG ATGATCCTGA AGCTGCGTAT 
ACAACACGGG GTGGAAAAAT CCCCATTCGA TGGACAGCCC CAGAGGCCAT CGCGTACAGA 
AAGTTCACCT CAGCGAGTGA CGTGTGGAGT TACGGCATCG TCATGTGGGA GGTGATTTCA 
TACGGAGAAC GGCCATATTG GGAGATGTCT AATCAAGACG TGATTAAAGC TATAGATGAG 
GGTTACAGGC TTCCCGCCCC AATGGACTGC CCCGTGGTCC TTCATCAGCT CATGCTGGAC 
TGCTGGGAGA AAAGCAGAAG TGAACGGCCC AAATTCGGGC AGATCGTCAA CACTCTTGAC 
AAACTTATCC GCAACCCCAA CAGTCTGAAG GAGCTCGCTA ACAGCTCAGT TTGGGAAGAC 
CCCACCACCC CAGAGTTCAG TGTGAGTACG GTGGATGAAT GGCTGGACGC CATCAATATG 
GGCCAGTACA AGGACAACTT TGCCAATGCG GGTTATGTTT CCCTAGATTC CATTCTGTAC 
ATCAGCATGT GTGAGTTGAG CAAGATGGAC GTGACTCTGG TGGGCCACCA AAAGAAGATT 
CTCTCCGCCG TCCAGAGTTT GCACGCACAG GGGACACACG TGCAGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

epha4b ( NM_153658.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_705944.1 Danio rerio eph receptor A4b (epha4b), mRNA.

Price & Availability↑

CloneID	ODa00442
Clone ID Related Accession (Same CDS sequence)	NM_153658.1
Accession Version	NM_153658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Danio rerio(zebrafish)
Product	eph receptor A4b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF369381.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF369381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505390, SAMEA4476729 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACCC TTTGGATTTA TCCGTCCCTT CTTTTCTTTC TCTTTCCCTC CCTCACACCG 
AGTCAAGCCT TACGGAATTA CCCAGAGAAT GAAGTCACAT TGCTGGACAC AATGTCTGCT 
CTGGGAGACC TTGGTTGGGA GGCCTATCCT GCTGAAGGGT GGGAGGAAAT CAGTGTGATG 
GATGAGAGAA ACACCCCCAT GCGTTCCTAC CAGGTTTGCA ACGTGATGGA GGCCAGTCAG 
AATAACTGGT TTCGCACTCG GCACATTGCC CGGGAAGGAG CCCAGCGGGT CTACATCGAG 
ATCAAGTTCA CCCTGCGCGA CTGCAACAGC CTGCCAGGAG TCCCCGGCAC CTGCAAAGAA 
ACCTTCAACA TGTACTACTA TGAGTCCAAC AACCCCAACC TGTGGTTCAT CAAGGAGAGC 
CAGTACATAA AGATTGACAC CATCGCCGCC GACGAGAGCT TCACACAGAC CGACGTCGGT 
GATCGGGTCA TGAGACTAAA CACGGAAATC CGGGACATCA ATAACTTGAG CAAGAAGGGT 
TTTTACCTTG CTTTCCAAGA TGTCGGAGCC TGCATCGCGC TGGTGTCTCT GAGGGTCTTT 
TATAAGAAGT GTCCTCTGGC GGTGTTGAAC TTGGCCCAGT TTCCCGATAC GGTGACTGGA 
GGAGATTCCG CTTTGGTGGA GGTTAGAGGG TCTTGTGTGA ATGATTCGGA GGAGTTTGAG 
GCTCCGAGGA TGTACTGCAG TGGAGATGGT GGATGGCTGG TGCCCATCGG ACGCTGCGTC 
TGCAAGCCGG GCTTCGAAGA GAATAAGGAC TACTGTCAGG CCTGCAGGCC AGGCACCTAC 
AAAGCCTCTT CCATGGACGC CTACTGCTCC AAATGTCCTC CACACAGTTT CTCTCACCAG 
GAAAAGGCTT CTGAATGTTC CTGTGAGAAG GGATTTTACA GAGCTGACAT GGACCCGCGG 
TCAATGGCCT GCACCCGGCC TCCGTCAGCA CCGGAGAACC CGATCTCCAC CCTGAACGAC 
ACCTCAGTGA CTTTAGAATG GAGTCCTCCT CGAGACAGCG GCGGACGAGG AGATGTGACC 
TACAGCATAC ACTGCAGAAA GTGTGCTGGC GACGGAAGGA TATGCAGCCC CTGCAGCAAC 
AATGTCCACT TTGTCCCAAG ACAGTTCGGC TTGTCGATCC CAAAAGTGCT GATCACAGAC 
CTGCTATCCA ACACCAACTA CACCTTCAGC GTTGAAGCGG TGAATGGGGT GTCAGATCTG 
AGCCCAACCC CTAAACAACT AGTCACAGTC AACATTACCA CCAGTCAGAC AGTGAATGTG 
ATCCTGAAGG AGAGAAAGAG TAAAGACAGT ATTACCCTGG CATGGCAGGG GCCCGACAGA 
CCAAACGGAG TCATAGTGGA ATATGAAGTC ATCTACTATG AGAAGAACCA AAGAGACCAG 
AACTACACAG TTCTGAAGAC CAAAGGGCAC ATGATGACTG TGGAGGGCCT GAAACCAGGA 
ACCACATACG TGTTCAGAGT GAGGGCTCGC ACTGATGGAG GATACGGCAA CTATGGTGGA 
GAGATCGAGC TGGAGACCAG TCATGAGGAT ATGCTGGCCA TTGGGGGACC GAACCAGTCC 
ACAATCCTAG CTGTGTCCAT AGCTGGAGGC ATTGTGCTGC TTACCTTCCT GGTTGCGTGT 
TTTGTAGTGA GCGGACGACA TTGTGGCTAC ATTAAAGCAA AGCAAGATCC AGATGAAGAA 
AAAATGCAGT TTCAGAACGG CAGAGTGAAG CTGCCAGGAA GCAGGACGTA CATTCACCCG 
CACACTTATG AGGACCCAAA TCAAGCCGTT CGGGATTTTG CTAAGGAAAT TGATGCCTCC 
ACTATACGTA TAGAGAGAGT TATTGGGGCA GGGGAGTTTG GTGAGGTGTG CAGTGGCCGT 
CTGAAGCTCC CCAGCAAGAG GGAAATCTAT GTGGCTATCA AGAGTCTGAA AGCAGGATAT 
TCAGAGAAAC AGAGGCGGGA CTTTCTGAGC GAGGCCAGCA TCATGGGCCA GTTCGACCAT 
CCCAACATCA TCCGACTGGA GGGAGTCGTC ACCAGATGCA AGCCCGTGAT GATTATTACA 
GAGTACATGG AGAATGGATC GTTGGATACT TTCCTGAAGA AGCATGATGG GCAGTTCACA 
GTCATTCAGC TGGTGGGCAT GATGCGTGGC ATCGCTTCAG GAATGAAATA TCTGTCTGAT 
ATGAGCTACG TCCACCGTGA TCTGGCAGCT CGCAACATCT TGGTCAACAG TAACCTGGTG 
TGCAAAGTGT CTGACTTTGG CCTGTCCAGA GTGCTGGAGG ATGATCCTGA AGCTGCGTAT 
ACAACACGGG GTGGAAAAAT CCCCATTCGA TGGACAGCCC CAGAGGCCAT CGCGTACAGA 
AAGTTCACCT CAGCGAGTGA CGTGTGGAGT TACGGCATCG TCATGTGGGA GGTGATTTCA 
TACGGAGAAC GGCCATATTG GGAGATGTCT AATCAAGACG TGATTAAAGC TATAGATGAG 
GGTTACAGGC TTCCCGCCCC AATGGACTGC CCCGTGGTCC TCCATCAGCT CATGCTGGAC 
TGCTGGGAGA AGAGCAGAAG TGAACGGCCC AAATTCGGGC AGATCGTCAA CACTCTTGAC 
AAACTTATCC GCAACCCCAA CAGTCTGAAA GAGCTCGCTA ACAGCTCAGT TTGGGAAGAC 
CCCACCACCC CAGAGTTCAG TGTGAGTACG GTGGATGAAT GGCTGGACGC CATCAATATG 
GGCCAGTACA AGGACAACTT TGCCAATGCG GGTTATGTTT CCCTAGATTC CATTCTGTAC 
ATCAGCATGT GTGAGTTGAG CAAGATGGAC GTGACTCTGG TGGGCCACCA AAAGAAGATT 
CTCTCCGCCG TCCAGAGTTT GCACGCACAG GGGACACACG TGCAGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705944.1
CDS	42..2972
Translation	MATLWIYPSLLFFLFPSLTPSQALRNYPENEVTLLDTMSALGDLGWEAYPAEGWEEISVMDERNTPMRSYQVCNVMEASQNNWFRTRHIAREGAQRVYIEIKFTLRDCNSLPGVPGTCKETFNMYYYESNNPNLWFIKESQYIKIDTIAADESFTQTDVGDRVMRLNTEIRDINNLSKKGFYLAFQDVGACIALVSLRVFYKKCPLAVLNLAQFPDTVTGGDSALVEVRGSCVNDSEEFEAPRMYCSGDGGWLVPIGRCVCKPGFEENKDYCQACRPGTYKASSMDAYCSKCPPHSFSHQEKASECSCEKGFYRADMDPRSMACTRPPSAPENPISTLNDTSVTLEWSPPRDSGGRGDVTYSIHCRKCAGDGRICSPCSNNVHFVPRQFGLSIPKVLITDLLSNTNYTFSVEAVNGVSDLSPTPKQLVTVNITTSQTVNVILKERKSKDSITLAWQGPDRPNGVIVEYEVIYYEKNQRDQNYTVLKTKGHMMTVEGLKPGTTYVFRVRARTDGGYGNYGGEIELETSHEDMLAIGGPNQSTILAVSIAGGIVLLTFLVACFVVSGRHCGYIKAKQDPDEEKMQFQNGRVKLPGSRTYIHPHTYEDPNQAVRDFAKEIDASTIRIERVIGAGEFGEVCSGRLKLPSKREIYVAIKSLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTRCKPVMIITEYMENGSLDTFLKKHDGQFTVIQLVGMMRGIASGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVISYGERPYWEMSNQDVIKAIDEGYRLPAPMDCPVVLHQLMLDCWEKSRSERPKFGQIVNTLDKLIRNPNSLKELANSSVWEDPTTPEFSVSTVDEWLDAINMGQYKDNFANAGYVSLDSILYISMCELSKMDVTLVGHQKKILSAVQSLHAQGTHVQV

Target ORF information:

RefSeq Version	NM_153658.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eph receptor A4b (epha4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153658.1

ORF Insert Sequence:

ATGGCTACCC TTTGGATTTA TCCGTCCCTT CTTTTCTTTC TCTTTCCCTC CCTCACACCG 
AGTCAAGCCT TACGGAATTA CCCAGAGAAT GAAGTCACAT TGCTGGACAC AATGTCTGCT 
CTGGGAGACC TTGGTTGGGA GGCCTATCCT GCTGAAGGGT GGGAGGAAAT CAGTGTGATG 
GATGAGAGAA ACACCCCCAT GCGTTCCTAC CAGGTTTGCA ACGTGATGGA GGCCAGTCAG 
AATAACTGGT TTCGCACTCG GCACATTGCC CGGGAAGGAG CCCAGCGGGT CTACATCGAG 
ATCAAGTTCA CCCTGCGCGA CTGCAACAGC CTGCCAGGAG TCCCCGGCAC CTGCAAAGAA 
ACCTTCAACA TGTACTACTA TGAGTCCAAC AACCCCAACC TGTGGTTCAT CAAGGAGAGC 
CAGTACATAA AGATTGACAC CATCGCCGCC GACGAGAGCT TCACACAGAC CGACGTCGGT 
GATCGGGTCA TGAGACTAAA CACGGAAATC CGGGACATCA ATAACTTGAG CAAGAAGGGT 
TTTTACCTTG CTTTCCAAGA TGTCGGAGCC TGCATCGCGC TGGTGTCTCT GAGGGTCTTT 
TATAAGAAGT GTCCTCTGGC GGTGTTGAAC TTGGCCCAGT TTCCCGATAC GGTGACTGGA 
GGAGATTCCG CTTTGGTGGA GGTTAGAGGG TCTTGTGTGA ATGATTCGGA GGAGTTTGAG 
GCTCCGAGGA TGTACTGCAG TGGAGATGGT GGATGGCTGG TGCCCATCGG ACGCTGCGTC 
TGCAAGCCGG GCTTCGAAGA GAATAAGGAC TACTGTCAGG CCTGCAGGCC AGGCACCTAC 
AAAGCCTCTT CCATGGACGC CTACTGCTCC AAATGTCCTC CACACAGTTT CTCTCACCAG 
GAAAAGGCTT CTGAATGTTC CTGTGAGAAG GGATTTTACA GAGCTGACAT GGACCCGCGG 
TCAATGGCCT GCACCCGGCC TCCGTCAGCA CCGGAGAACC CGATCTCCAC CCTGAACGAC 
ACCTCAGTGA CTTTAGAATG GAGTCCTCCT CGAGACAGCG GCGGACGAGG AGATGTGACC 
TACAGCATAC ACTGCAGAAA GTGTGCTGGC GACGGAAGGA TATGCAGCCC CTGCAGCAAC 
AATGTCCACT TTGTCCCAAG ACAGTTCGGC TTGTCGATCC CAAAAGTGCT GATCACAGAC 
CTGCTATCCA ACACCAACTA CACCTTCAGC GTTGAAGCGG TGAATGGGGT GTCAGATCTG 
AGCCCAACCC CTAAACAACT AGTCACAGTC AACATTACCA CCAGTCAGAC AGTGAATGTG 
ATCCTGAAGG AGAGAAAGAG TAAAGACAGT ATTACCCTGG CATGGCAGGG GCCCGACAGA 
CCAAACGGAG TCATAGTGGA ATATGAAGTC ATCTACTATG AGAAGAACCA AAGAGACCAG 
AACTACACAG TTCTGAAGAC CAAAGGGCAC ATGATGACTG TGGAGGGCCT GAAACCAGGA 
ACCACATACG TGTTCAGAGT GAGGGCTCGC ACTGATGGAG GATACGGCAA CTATGGTGGA 
GAGATCGAGC TGGAGACCAG TCATGAGGAT ATGCTGGCCA TTGGGGGACC GAACCAGTCC 
ACAATCCTAG CTGTGTCCAT AGCTGGAGGC ATTGTGCTGC TTACCTTCCT GGTTGCGTGT 
TTTGTAGTGA GCGGACGACA TTGTGGCTAC ATTAAAGCAA AGCAAGATCC AGATGAAGAA 
AAAATGCAGT TTCAGAACGG CAGAGTGAAG CTGCCAGGAA GCAGGACGTA CATTCACCCG 
CACACTTATG AGGACCCAAA TCAAGCCGTT CGGGATTTTG CTAAGGAAAT TGATGCCTCC 
ACTATACGTA TAGAGAGAGT TATTGGGGCA GGGGAGTTTG GTGAGGTGTG CAGTGGCCGT 
CTGAAGCTCC CCAGCAAGAG GGAAATCTAT GTGGCTATCA AGAGTCTGAA AGCAGGATAT 
TCAGAGAAAC AGAGGCGGGA CTTTCTGAGC GAGGCCAGCA TCATGGGCCA GTTCGACCAT 
CCCAACATCA TCCGACTGGA GGGAGTCGTC ACCAGATGCA AGCCCGTGAT GATTATTACA 
GAGTACATGG AGAATGGATC GTTGGATACT TTCCTGAAGA AGCATGATGG GCAGTTCACA 
GTCATTCAGC TGGTGGGCAT GATGCGTGGC ATCGCTTCAG GAATGAAATA TCTGTCTGAT 
ATGAGCTACG TCCACCGTGA TCTGGCAGCT CGCAACATCT TGGTCAACAG TAACCTGGTG 
TGCAAAGTGT CTGACTTTGG CCTGTCCAGA GTGCTGGAGG ATGATCCTGA AGCTGCGTAT 
ACAACACGGG GTGGAAAAAT CCCCATTCGA TGGACAGCCC CAGAGGCCAT CGCGTACAGA 
AAGTTCACCT CAGCGAGTGA CGTGTGGAGT TACGGCATCG TCATGTGGGA GGTGATTTCA 
TACGGAGAAC GGCCATATTG GGAGATGTCT AATCAAGACG TGATTAAAGC TATAGATGAG 
GGTTACAGGC TTCCCGCCCC AATGGACTGC CCCGTGGTCC TCCATCAGCT CATGCTGGAC 
TGCTGGGAGA AGAGCAGAAG TGAACGGCCC AAATTCGGGC AGATCGTCAA CACTCTTGAC 
AAACTTATCC GCAACCCCAA CAGTCTGAAA GAGCTCGCTA ACAGCTCAGT TTGGGAAGAC 
CCCACCACCC CAGAGTTCAG TGTGAGTACG GTGGATGAAT GGCTGGACGC CATCAATATG 
GGCCAGTACA AGGACAACTT TGCCAATGCG GGTTATGTTT CCCTAGATTC CATTCTGTAC 
ATCAGCATGT GTGAGTTGAG CAAGATGGAC GTGACTCTGG TGGGCCACCA AAAGAAGATT 
CTCTCCGCCG TCCAGAGTTT GCACGCACAG GGGACACACG TGCAGGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ephrin-A1-EphA4 signaling negatively regulates myelination in the central nervous system.
Glia(2018 Jan)
Harboe M,Torvund-Jensen J,Kjaer-Sorensen K,Laursen LS

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Toxicology and applied pharmacology29184-96(2016 Jan)
Bouwmeester MC,Ruiter S,Lommelaars T,Sippel J,Hodemaekers HM,van den Brandhof EJ,Pennings JL,Kamstra JH,Jelinek J,Issa JP,Legler J,van der Ven LT

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epha4b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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