ptpn20 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009307421.2	XP_009305696.1	tyrosine-protein phosphatase non-receptor type 13 isoform X1
XM_009307424.2	XP_009305699.1	FERM and PDZ domain-containing protein 2 isoform X4
XM_009307422.3	XP_009305697.1	tyrosine-protein phosphatase non-receptor type 13 isoform X2
XM_002664188.5	XP_002664234.3	tyrosine-protein phosphatase non-receptor type 13 isoform X1
XM_009307422.2	XP_009305697.1	tyrosine-protein phosphatase non-receptor type 13 isoform X2
XM_009307423.2	XP_009305698.1	tyrosine-protein phosphatase non-receptor type 13 isoform X3
XM_009307421.3	XP_009305696.1	tyrosine-protein phosphatase non-receptor type 13 isoform X1
XM_009307423.3	XP_009305698.1	tyrosine-protein phosphatase non-receptor type 13 isoform X3
XM_002664188.6	XP_002664234.3	tyrosine-protein phosphatase non-receptor type 13 isoform X1
XM_009307424.3	XP_009305699.1	FERM and PDZ domain-containing protein 2 isoform X4
XM_021480875.1	XP_021336550.1	tyrosine-protein phosphatase non-receptor type 20 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148646	XM_021480875.1 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa46182	XM_009307424.3 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18920	XM_002664188.6 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa18920	XM_009307421.3 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa46180	XM_009307422.3 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa46181	XM_009307423.3 Latest version!	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa46180	XM_009307422.2	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa18920	XM_009307421.2	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa46181	XM_009307423.2	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa18920	XM_002664188.5	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa46182	XM_009307424.2	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ptpn20 ( XM_021480875.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336550.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa148646
Clone ID Related Accession (Same CDS sequence)	XM_021480875.1
Accession Version	XM_021480875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 20 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGGAGAGGGG CATTTTTAAA GCAGTCGCTG ACAGTGCCAT GCTGTTCAGG 
AAGAGGCTGT CGGTCACAGC GGAGCAGGAG AGCTGTATCC TGCAGGTGGA GTTCACCAAG 
CCTGATCGTG GTGGGCTGGG CTTTGCACTG GTGGGCGGAG TCAACGGCAG CACACTGAGA 
GTGAAGGACA TATGCTGTGG GGGCGTGGCT GAGCAGGACG GTCGTCTTCG AGTGGGTGAC 
ATCCTTCTGG AGGTTAATGG CATCATTGTA TCAGGACTGA GCCATAGTAA AGTTGTGGAT 
ATTTTGAGGA AGGCGGAGGG CACGGTTCAG CTGACGGTGT GTAGGGACAT CCTGCCCATG 
TCCGCCTGCT CTGCTTCCAA CTACCCCACT GAGGCCCATG GGAATTTCAA CACACTGAAT 
TCTGCAGCAC AAACTGAAGC ACATCCAGAT CACGTGTCTT TCCACTCAGA CTGTCCTGAA 
TGTGAGCAGG AAAGTCGAAA CTGTACACCT ACCTGTCAGA GGTGTTGTCC GTCTCTGGGA 
GTCACAGACA TGCTACAAGA AAAGACAGAC CCCCAGAAGC AGCATCTAGA AAATTCTGCC 
ATGAAAACAG AGCACAGCCA GTCGGACGGC TGGAGCAGTG ACGAGGAAGA AGATGGTGAC 
GATGACGACT GTGCTTCCCA GCCTTCGCCT CCAGAGACTA TACCTCTGAC AGGTCGGCCA 
ATCGTGTCAG AAGAGGAGCT TTCCAGTCTT GCAGTGATTT CTCCTCCTAA GAATGGGCAG 
TACACCGGCT CCAGGGTCAA AGCCCTCATC CAGGCCCTCG AGCAGCTAGA AAAGCAAGAT 
CTGGTCAAAG AGTTTATGGC TTTGGAGCAT TTAAACCCTT CAGACAACTG CCTGGTTGGA 
AAAGCACCTG AAAACCGGGA AAAGAACAGA TACAGGGAAA TCCTGCCTTA CGATAAGACG 
CGTGTTCCTG TGGGAGAGGA CGAGGGCTAC ATCAATGCCA GCTACATTCG TATGAAGGTG 
GGCTCGGAGG AGCTGTTCTT CATCTCCGCT CAGGGCCCTT TGCCTGGCAC TCAGGACAGT 
TTCTGGCAGA TGGTGTGGGA GAACAGGTCT GATGTGATCG CCATGATGAC TCGAGAGGTG 
GAGCGCGGAC GGGTCAAATG CCACAAATAC TGGCCCGAGA AGCTCGACGT CCCCAAAGAG 
ACCAGCCGTT ACCAGCTCTT CCTGGACAAC TACCAGATGC TTGGTTATTT CCACATTAAG 
GTCATCAAGA TGGTGGAAAA AAAGAGTGGA GACGTGCACT TTGTGAAGCA CCTGAAGTTC 
ACCACATGGC CTGATCACGG CACACCCCAC TCCTCCGATC AGCTTGTGCG CTTCATCCGC 
TACATGAGGG CTGTTCACGC TAAAGGGCCC ATCACCGTCC ACTGCAGCGC AGGCATCGGC 
CGAGCCGGAG TCCTCATCTG TACAGACGTC ATCCTCAGTC TCATTCAGAA AGACCTCAGT 
ATTAATGTGA GTGACATTGT AAAGGAGATG AGACTCCAGC GGCACGGCAT GATTCAGACT 
AAGGAACAAT ACCTCTTTTG TTATAAAGTC TGGTTGGAAG TGTTACAAAA CATCTCGCTC 
CTTTATGGTC ACCACTGGCA AACCGAAACC TCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336550.1
CDS	61..1719
Translation	MAAKERGIFKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_021480875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480875.1

ORF Insert Sequence:

ATGGCAGCAA AGGAGAGGGG CATTTTTAAA GCAGTCGCTG ACAGTGCCAT GCTGTTCAGG 
AAGAGGCTGT CGGTCACAGC GGAGCAGGAG AGCTGTATCC TGCAGGTGGA GTTCACCAAG 
CCTGATCGTG GTGGGCTGGG CTTTGCACTG GTGGGCGGAG TCAACGGCAG CACACTGAGA 
GTGAAGGACA TATGCTGTGG GGGCGTGGCT GAGCAGGACG GTCGTCTTCG AGTGGGTGAC 
ATCCTTCTGG AGGTTAATGG CATCATTGTA TCAGGACTGA GCCATAGTAA AGTTGTGGAT 
ATTTTGAGGA AGGCGGAGGG CACGGTTCAG CTGACGGTGT GTAGGGACAT CCTGCCCATG 
TCCGCCTGCT CTGCTTCCAA CTACCCCACT GAGGCCCATG GGAATTTCAA CACACTGAAT 
TCTGCAGCAC AAACTGAAGC ACATCCAGAT CACGTGTCTT TCCACTCAGA CTGTCCTGAA 
TGTGAGCAGG AAAGTCGAAA CTGTACACCT ACCTGTCAGA GGTGTTGTCC GTCTCTGGGA 
GTCACAGACA TGCTACAAGA AAAGACAGAC CCCCAGAAGC AGCATCTAGA AAATTCTGCC 
ATGAAAACAG AGCACAGCCA GTCGGACGGC TGGAGCAGTG ACGAGGAAGA AGATGGTGAC 
GATGACGACT GTGCTTCCCA GCCTTCGCCT CCAGAGACTA TACCTCTGAC AGGTCGGCCA 
ATCGTGTCAG AAGAGGAGCT TTCCAGTCTT GCAGTGATTT CTCCTCCTAA GAATGGGCAG 
TACACCGGCT CCAGGGTCAA AGCCCTCATC CAGGCCCTCG AGCAGCTAGA AAAGCAAGAT 
CTGGTCAAAG AGTTTATGGC TTTGGAGCAT TTAAACCCTT CAGACAACTG CCTGGTTGGA 
AAAGCACCTG AAAACCGGGA AAAGAACAGA TACAGGGAAA TCCTGCCTTA CGATAAGACG 
CGTGTTCCTG TGGGAGAGGA CGAGGGCTAC ATCAATGCCA GCTACATTCG TATGAAGGTG 
GGCTCGGAGG AGCTGTTCTT CATCTCCGCT CAGGGCCCTT TGCCTGGCAC TCAGGACAGT 
TTCTGGCAGA TGGTGTGGGA GAACAGGTCT GATGTGATCG CCATGATGAC TCGAGAGGTG 
GAGCGCGGAC GGGTCAAATG CCACAAATAC TGGCCCGAGA AGCTCGACGT CCCCAAAGAG 
ACCAGCCGTT ACCAGCTCTT CCTGGACAAC TACCAGATGC TTGGTTATTT CCACATTAAG 
GTCATCAAGA TGGTGGAAAA AAAGAGTGGA GACGTGCACT TTGTGAAGCA CCTGAAGTTC 
ACCACATGGC CTGATCACGG CACACCCCAC TCCTCCGATC AGCTTGTGCG CTTCATCCGC 
TACATGAGGG CTGTTCACGC TAAAGGGCCC ATCACCGTCC ACTGCAGCGC AGGCATCGGC 
CGAGCCGGAG TCCTCATCTG TACAGACGTC ATCCTCAGTC TCATTCAGAA AGACCTCAGT 
ATTAATGTGA GTGACATTGT AAAGGAGATG AGACTCCAGC GGCACGGCAT GATTCAGACT 
AAGGAACAAT ACCTCTTTTG TTATAAAGTC TGGTTGGAAG TGTTACAAAA CATCTCGCTC 
CTTTATGGTC ACCACTGGCA AACCGAAACC TCTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307424.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305699.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa46182
Clone ID Related Accession (Same CDS sequence)	XM_009307424.2 , XM_009307424.3
Accession Version	XM_009307424.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	FERM and PDZ domain-containing protein 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGTGGAAAA ATCGCAGGAA AATCAGCCTT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305699.1
CDS	295..5820
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKWKNRRKISLA

Target ORF information:

RefSeq Version	XM_009307424.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307424.2

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGTGGAAAA ATCGCAGGAA AATCAGCCTT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307424.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305699.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.

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CloneID	ODa46182
Clone ID Related Accession (Same CDS sequence)	XM_009307424.2 , XM_009307424.3
Accession Version	XM_009307424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FERM and PDZ domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGTGGAAAA ATCGCAGGAA AATCAGCCTT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305699.1
CDS	308..5833
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKWKNRRKISLA

Target ORF information:

RefSeq Version	XM_009307424.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307424.3

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGTGGAAAA ATCGCAGGAA AATCAGCCTT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307421.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305696.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.

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CloneID	ODa18920
Clone ID Related Accession (Same CDS sequence)	XM_009307421.2 , XM_002664188.5 , XM_009307421.3 , XM_002664188.6
Accession Version	XM_009307421.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305696.1
CDS	111..6698
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307421.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307421.2

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_002664188.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664234.3 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.

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CloneID	ODa18920
Clone ID Related Accession (Same CDS sequence)	XM_009307421.2 , XM_002664188.5 , XM_009307421.3 , XM_002664188.6
Accession Version	XM_002664188.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002664188.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664234.3
CDS	295..6882
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_002664188.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664188.5

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307421.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305696.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.

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CloneID	ODa18920
Clone ID Related Accession (Same CDS sequence)	XM_009307421.2 , XM_002664188.5 , XM_009307421.3 , XM_002664188.6
Accession Version	XM_009307421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305696.1
CDS	154..6741
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307421.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307421.3

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_002664188.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002664234.3 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.

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CloneID	ODa18920
Clone ID Related Accession (Same CDS sequence)	XM_009307421.2 , XM_002664188.5 , XM_009307421.3 , XM_002664188.6
Accession Version	XM_002664188.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002664188.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002664234.3
CDS	306..6893
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_002664188.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002664188.6

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG GTTTGTTTGA ATGGAAAGAG AAGAAAGGTT TCCCTCACCT GGAGGAGCCC 
ATGTCACCTG ACGTGCATTC AGCTCTTGCA GACGTTAAAG CAGTCGCTGA CAGTGCCATG 
CTGTTCAGGA AGAGGCTGTC GGTCACAGCG GAGCAGGAGA GCTGTATCCT GCAGGTGGAG 
TTCACCAAGC CTGATCGTGG TGGGCTGGGC TTTGCACTGG TGGGCGGAGT CAACGGCAGC 
ACACTGAGAG TGAAGGACAT ATGCTGTGGG GGCGTGGCTG AGCAGGACGG TCGTCTTCGA 
GTGGGTGACA TCCTTCTGGA GGTTAATGGC ATCATTGTAT CAGGACTGAG CCATAGTAAA 
GTTGTGGATA TTTTGAGGAA GGCGGAGGGC ACGGTTCAGC TGACGGTGTG TAGGGACATC 
CTGCCCATGT CCGCCTGCTC TGCTTCCAAC TACCCCACTG AGGCCCATGG GAATTTCAAC 
ACACTGAATT CTGCAGCACA AACTGAAGCA CATCCAGATC ACGTGTCTTT CCACTCAGAC 
TGTCCTGAAT GTGAGCAGGA AAGTCGAAAC TGTACACCTA CCTGTCAGAG GTGTTGTCCG 
TCTCTGGGAG TCACAGACAT GCTACAAGAA AAGACAGACC CCCAGAAGCA GCATCTAGAA 
AATTCTGCCA TGAAAACAGA GCACAGCCAG TCGGACGGCT GGAGCAGTGA CGAGGAAGAA 
GATGGTGACG ATGACGACTG TGCTTCCCAG CCTTCGCCTC CAGAGACTAT ACCTCTGACA 
GGTCGGCCAA TCGTGTCAGA AGAGGAGCTT TCCAGTCTTG CAGTGATTTC TCCTCCTAAG 
AATGGGCAGT ACACCGGCTC CAGGGTCAAA GCCCTCATCC AGGCCCTCGA GCAGCTAGAA 
AAGCAAGATC TGGTCAAAGA GTTTATGGCT TTGGAGCATT TAAACCCTTC AGACAACTGC 
CTGGTTGGAA AAGCACCTGA AAACCGGGAA AAGAACAGAT ACAGGGAAAT CCTGCCTTAC 
GATAAGACGC GTGTTCCTGT GGGAGAGGAC GAGGGCTACA TCAATGCCAG CTACATTCGT 
ATGAAGGTGG GCTCGGAGGA GCTGTTCTTC ATCTCCGCTC AGGGCCCTTT GCCTGGCACT 
CAGGACAGTT TCTGGCAGAT GGTGTGGGAG AACAGGTCTG ATGTGATCGC CATGATGACT 
CGAGAGGTGG AGCGCGGACG GGTCAAATGC CACAAATACT GGCCCGAGAA GCTCGACGTC 
CCCAAAGAGA CCAGCCGTTA CCAGCTCTTC CTGGACAACT ACCAGATGCT TGGTTATTTC 
CACATTAAGG TCATCAAGAT GGTGGAAAAA AAGAGTGGAG ACGTGCACTT TGTGAAGCAC 
CTGAAGTTCA CCACATGGCC TGATCACGGC ACACCCCACT CCTCCGATCA GCTTGTGCGC 
TTCATCCGCT ACATGAGGGC TGTTCACGCT AAAGGGCCCA TCACCGTCCA CTGCAGCGCA 
GGCATCGGCC GAGCCGGAGT CCTCATCTGT ACAGACGTCA TCCTCAGTCT CATTCAGAAA 
GACCTCAGTA TTAATGTGAG TGACATTGTA AAGGAGATGA GACTCCAGCG GCACGGCATG 
ATTCAGACTA AGGAACAATA CCTCTTTTGT TATAAAGTCT GGTTGGAAGT GTTACAAAAC 
ATCTCGCTCC TTTATGGTCA CCACTGGCAA ACCGAAACCT CTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307422.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305697.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.

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CloneID	ODa46180
Clone ID Related Accession (Same CDS sequence)	XM_009307422.3 , XM_009307422.2
Accession Version	XM_009307422.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307422.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGACGAAGC 
ACAAGTTTTT GGTCACGAAG TTCGTGTCTA AACTCTCCAA ACAAAGGTGA AGTCCGACCT 
TCCAGTCCCT GCATTACTGT GAGTGAGTCT GCAGTCAGTC TGTCCCAAAG AAAAGCTAAG 
AGTCTTGGAC CTGAGTTTAC CAGGATGAAC GATGAGCCTC AAATCATTTT AGAGCTGCCG 
GGGTCTATAG TGTCGAAGAA AGGCAAGTCA GGTCTGTCTC AGAGGGAGGT TAACGTGATC 
ATGCCAAATG AACAGTGTGT GGCAGTGAAA TGTGACATCA AGTCCCGAGG GAGGGATGTG 
TTCGACATGG TTGTGGCACA CGCTAACCTT GTGGAGCATT TTTACTTTGG CCTTGCTTTC 
ATTGATGATG GTGAATTTTT CTTCTTGGAC CCCGAGACAA AAATCTCAAA GGTTGCTCCT 
GATTACTGGA AGAAAGTGGC TTCTGCGACA TTTACACTTT ATCTTCGTGT CAGATTTTTC 
CCTGATGACA TCTCCTACAT TCTACATAAA CATACTCGAC ACCAATATTA TCTCCAGCTG 
AGGAAAGATA TTCTGGAAGA CAGAGTGCAC TGTAATGAGG AGACGGCGCT GTTTCTCGCT 
GCGCTGGCAC TGCAGGCTGA GTTTGGAGAT TATATGGCTG AAGTTTACGG GAAGAACTAT 
TTCCAGACGG AGCAGTACAT CTCTAAGAGA GTCATAGAGA AAGTGGCCTT GCCCTGTTTG 
AGAGAGGAAT TGCCAAGGTT ACATGCCAAT AATGCCCAAA TGCTTCCAGA GGAGGCAGAG 
ACGGAGTTCT TAAAGATTGC ACAGCAACTA CCAGAGTATG GGGTGCTGTT TCACAGAGTT 
GCCCGTGAAC GGAAACCTAT TATTGGAGAG CTGGTTTTGG GAATTTGTGC CAAAGGAATT 
CTGGTTTATG AAGTCAAGAA TAATTCTCGG ATGTTAAGCC GCAGATTTCA CTGGACAGAA 
ACTGACAGTC TCTCAACAAG TAGACGAAAA TTTACCATTG AGTGCAGCCC AAGTGGAAAG 
AAGCACTCGT TTGTGACGGA GAGCTCAAAA ATCGCACAGT ACCTCCTGAA CCTGTGCTCG 
GCCCAACACA AGTTTCACAG TGAGATGACC TCACGCCAGC TGACCTACTG CTTAGCTTCA 
GATGACAGCG TAGCAAAATA TACATCCATA TGCCGTGCTC GACACGAGCA GATAAAGAGG 
CTGTCCTGCT CAGAGATTGT GCTCAATAAT ATTGGTCTGA ATGGATTGCC AAGTGACTCT 
ATAAGCAAGT CTTGCGATGA CCTGTCGGCG AAAATTGGAG TTCGGATCCG GCAACAGAAT 
GAAGTAAATT ACAGTCCTGA ATCGAGAGCA CCCTGTGAAC CAAAAGATCA GAGCCAAAGC 
AGCAGCCCAC TGGAAACCTT CAGTCCCCCA GTCTATCGCA TCATGTCCAA TGTCTCTTTG 
CAAAAGCAGG ACTCAGAGGT TCATTCCACT ACCTCATCTA TAAGAGTGGA CACACCCACA 
AAAACAACCC CAGAGAGAGA AATCATCTGT GTCACTTTAA AGAAGGATCC CAAACTTGGC 
TATGGTTTCA TAATAGTCGG GGAGGACAAC ACAGGGAAGT TAGATCTTGG AATTTTTATT 
GCATCCATCA TTCCCGATGG TCCAGCTGAT AAGGATGGGA GAATTAGACC AGGTGGTCGG 
CTGATCTCTC TGAATAACAT CAGTCTAGAA GGGGTGACTT TTAATATAGC AGCTTCCATA 
CTCCAGAACA GCCCAGAAGA AGTGGAGCTT ATAGTGTCTC AACCCAAACA GGATCTTCGG 
GGCAGCAAGA CTTCACTGGC TCCAAGTAAT TTAGGAATGA TGCTAGAAAG AAGCTTTGGC 
TCCCAAAGCA CACTGAGCGC CGAGTACAGA CCTGTGATGG AGGAGCTGGA GGAGACCCTC 
TCCAACATCA TGGCCCCTAA AATCAACAAA CGGATGCACA TACCTGTGGT CCGCATTCTG 
GATATTCAGG ACGAGATGTC TAAAAGTTCT TCTGGCTTCA GTTTGAAACC AGGTGAAATA 
ATCTACATGG AGCTCCGAAA GATCAACGGC AGTTTGGGAA TCAGTGTTGC TGGTGGGATC 
AACACTAATG TACATCATGG AGGAATCTAC ATCAAAAGTG TGATAGCAGG AGGAGCGGCA 
GATCAAGATG GAAGAATACA GATAGGTGAC AGACTGCTGG AGGTGGACGG ATGTAACCTG 
CGTGCCGTCA CACACAGGCA GGCGGTGGAG TGCCTGAAGA GAACCGGAGA GGTGGTGAGT 
CTGGTTCTGG AGCGAGAGCC TCCGGTGGTG CTGGACACTT CCAGTGCCTC ATCCACATTG 
GAGAGACGAA GATCTCCTTC ACCCCTCAGT GCCCCCCTGA GGAGAGACGT GCCCCTACAA 
ACGCCCTTGT CTGTCAGGAC CAAGGACTTT AGATTTGTGT CAGATGAAAA TATCCTGGAA 
GTGACGTTGC AGAAGAGTTT CAGTGGCCTG GGCTTCAGTT TTCTCGTATC TGAGTTGGGT 
CCTGAAAAGG AAAACATTGT GCAGATCAAG AGTCTTTCCT CTGGTCATCC TGCAGGGGAT 
TGTGGCCTTC TGAAGGAGGG AGACATAATC CTGGCTGTCA ATGGAGAACC TGTCAGAGGC 
CTTTCCTATG AGGAGGTTCT GCATCTCCTC CAAAAATCTC CATCTGAGAT CAGACTGTCA 
ATCTGCAGAC CTTCACAGGA TGAGCATACA GATATTGAGA CCTTTCTGGT TCCAAACCGT 
GAACTGAGAT CCAGATCTCT CGACCTACAA GCGAGGTCTG TGGCTCCAGA TTTCAGTGAG 
CTCCTCTTGA GGCGGGCGAT AGAGATGGGG CTTAAAAAAG AAGCTTCACC TGAGGAAACC 
CAGGAGAGTG AGCTGCTGGA AACAGATAAA TCAATTCCTC CACCTCTACC TCCACGTCCA 
TCTTCTGCTG AAGGTGGAGA AAGAAAACCT CATGTCATTC TAGTGGAGGA GTATGAGAAG 
AAAGACCCGC CTCCTCCTCC GACTCCTCCT CCAACACCAG GCATTCTTAA AGCGTCAACA 
ACCAACACTG TGCAAACCCC AGCAGCACCT AAAACTGCAA TTAAAGCCCA TCAGCAAGAG 
TCACTCGAAC CAACTGTCTC TAGAAGCAAC CATGTCTCAG CATGTACGAG TCACGTGAAA 
GATGAAGGAG TGGAAGAGAA CGTGATAAGT TTCACAGCTA ATGGTCTGAC CGTCACTGCT 
GGTGAAGAAT ACCTGACCAT CAGCACTACG GCTCCATCTC CCCCGAGCGG CAGCACTGCA 
GTCTCCTCTG AAGCTCCTCC TCCTGTGCCT GCACTTTCTC CATCACTGCT GAACACACCT 
CCAACATACC AGCAGGCCAT TCAAGCAGCA TCTCCAGCAG AGCCTCCTGC CCAGCCCAGC 
AACAACGGCT GGGATGATGA TGACGAAGAG GATGATGAAG ATCCCAGAAG GGATGTGTTT 
AAGGAGTTTG AACTGACCGT CTCACTGACC AAATCCTGGT GTGGAAGCTT CGGTTTTACA 
ATAACCAGGA GTAAATTGGA CAACTGCTAT TATGTCCAGG ATGTCCTGGA TAATCCAGCC 
AAAGCAGATG GCAGACTGAG GGCAGGAGAC AGGCTTATCA TGGTGAATGG TCATAATGTG 
ACCAGCGTGA CAGATGACGT GGCAATAAGC ATCCTCAGGT GCTCCTCCAA AAGACTGCAC 
ATGGTGCTGG GCAGAGCTGT GCAAAATCTG CTCCCTCCAC CACCTCCTGA CACCCTCCAG 
GACATTATCA TATCCAAGAC TCCATCTGGA CAACTGGGTA TTAAGCTAAC AGGAGGGATT 
GGAAGCAAAC GGCAAGGAAT TTATGTGCAG GAGGTGGTGC TGAGCTCTCC TGCTAGTGAG 
GAGGGCAGCA TTCAGCCCAA TGACAAGATT GTTTACATAT GTGGGAGATG CACTCTGGGA 
ATGACGCTGG AGGATGCGGT AAAAGTGTGT GAGAGCTCCC CACGCAAAGT CAGATTCAAA 
GCCATGAGAG ATGATCAACC AGTCATACCA AGAGATAAGT GGAACGGTTT GTTTGAATGG 
AAAGAGAAGA AAGGTTTCCC TCACCTGGAG GAGCCCATGT CACCTGACGT GCATTCAGCT 
CTTGCAGACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305697.1
CDS	306..6869
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307422.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307422.3

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGACGAAGC 
ACAAGTTTTT GGTCACGAAG TTCGTGTCTA AACTCTCCAA ACAAAGGTGA AGTCCGACCT 
TCCAGTCCCT GCATTACTGT GAGTGAGTCT GCAGTCAGTC TGTCCCAAAG AAAAGCTAAG 
AGTCTTGGAC CTGAGTTTAC CAGGATGAAC GATGAGCCTC AAATCATTTT AGAGCTGCCG 
GGGTCTATAG TGTCGAAGAA AGGCAAGTCA GGTCTGTCTC AGAGGGAGGT TAACGTGATC 
ATGCCAAATG AACAGTGTGT GGCAGTGAAA TGTGACATCA AGTCCCGAGG GAGGGATGTG 
TTCGACATGG TTGTGGCACA CGCTAACCTT GTGGAGCATT TTTACTTTGG CCTTGCTTTC 
ATTGATGATG GTGAATTTTT CTTCTTGGAC CCCGAGACAA AAATCTCAAA GGTTGCTCCT 
GATTACTGGA AGAAAGTGGC TTCTGCGACA TTTACACTTT ATCTTCGTGT CAGATTTTTC 
CCTGATGACA TCTCCTACAT TCTACATAAA CATACTCGAC ACCAATATTA TCTCCAGCTG 
AGGAAAGATA TTCTGGAAGA CAGAGTGCAC TGTAATGAGG AGACGGCGCT GTTTCTCGCT 
GCGCTGGCAC TGCAGGCTGA GTTTGGAGAT TATATGGCTG AAGTTTACGG GAAGAACTAT 
TTCCAGACGG AGCAGTACAT CTCTAAGAGA GTCATAGAGA AAGTGGCCTT GCCCTGTTTG 
AGAGAGGAAT TGCCAAGGTT ACATGCCAAT AATGCCCAAA TGCTTCCAGA GGAGGCAGAG 
ACGGAGTTCT TAAAGATTGC ACAGCAACTA CCAGAGTATG GGGTGCTGTT TCACAGAGTT 
GCCCGTGAAC GGAAACCTAT TATTGGAGAG CTGGTTTTGG GAATTTGTGC CAAAGGAATT 
CTGGTTTATG AAGTCAAGAA TAATTCTCGG ATGTTAAGCC GCAGATTTCA CTGGACAGAA 
ACTGACAGTC TCTCAACAAG TAGACGAAAA TTTACCATTG AGTGCAGCCC AAGTGGAAAG 
AAGCACTCGT TTGTGACGGA GAGCTCAAAA ATCGCACAGT ACCTCCTGAA CCTGTGCTCG 
GCCCAACACA AGTTTCACAG TGAGATGACC TCACGCCAGC TGACCTACTG CTTAGCTTCA 
GATGACAGCG TAGCAAAATA TACATCCATA TGCCGTGCTC GACACGAGCA GATAAAGAGG 
CTGTCCTGCT CAGAGATTGT GCTCAATAAT ATTGGTCTGA ATGGATTGCC AAGTGACTCT 
ATAAGCAAGT CTTGCGATGA CCTGTCGGCG AAAATTGGAG TTCGGATCCG GCAACAGAAT 
GAAGTAAATT ACAGTCCTGA ATCGAGAGCA CCCTGTGAAC CAAAAGATCA GAGCCAAAGC 
AGCAGCCCAC TGGAAACCTT CAGTCCCCCA GTCTATCGCA TCATGTCCAA TGTCTCTTTG 
CAAAAGCAGG ACTCAGAGGT TCATTCCACT ACCTCATCTA TAAGAGTGGA CACACCCACA 
AAAACAACCC CAGAGAGAGA AATCATCTGT GTCACTTTAA AGAAGGATCC CAAACTTGGC 
TATGGTTTCA TAATAGTCGG GGAGGACAAC ACAGGGAAGT TAGATCTTGG AATTTTTATT 
GCATCCATCA TTCCCGATGG TCCAGCTGAT AAGGATGGGA GAATTAGACC AGGTGGTCGG 
CTGATCTCTC TGAATAACAT CAGTCTAGAA GGGGTGACTT TTAATATAGC AGCTTCCATA 
CTCCAGAACA GCCCAGAAGA AGTGGAGCTT ATAGTGTCTC AACCCAAACA GGATCTTCGG 
GGCAGCAAGA CTTCACTGGC TCCAAGTAAT TTAGGAATGA TGCTAGAAAG AAGCTTTGGC 
TCCCAAAGCA CACTGAGCGC CGAGTACAGA CCTGTGATGG AGGAGCTGGA GGAGACCCTC 
TCCAACATCA TGGCCCCTAA AATCAACAAA CGGATGCACA TACCTGTGGT CCGCATTCTG 
GATATTCAGG ACGAGATGTC TAAAAGTTCT TCTGGCTTCA GTTTGAAACC AGGTGAAATA 
ATCTACATGG AGCTCCGAAA GATCAACGGC AGTTTGGGAA TCAGTGTTGC TGGTGGGATC 
AACACTAATG TACATCATGG AGGAATCTAC ATCAAAAGTG TGATAGCAGG AGGAGCGGCA 
GATCAAGATG GAAGAATACA GATAGGTGAC AGACTGCTGG AGGTGGACGG ATGTAACCTG 
CGTGCCGTCA CACACAGGCA GGCGGTGGAG TGCCTGAAGA GAACCGGAGA GGTGGTGAGT 
CTGGTTCTGG AGCGAGAGCC TCCGGTGGTG CTGGACACTT CCAGTGCCTC ATCCACATTG 
GAGAGACGAA GATCTCCTTC ACCCCTCAGT GCCCCCCTGA GGAGAGACGT GCCCCTACAA 
ACGCCCTTGT CTGTCAGGAC CAAGGACTTT AGATTTGTGT CAGATGAAAA TATCCTGGAA 
GTGACGTTGC AGAAGAGTTT CAGTGGCCTG GGCTTCAGTT TTCTCGTATC TGAGTTGGGT 
CCTGAAAAGG AAAACATTGT GCAGATCAAG AGTCTTTCCT CTGGTCATCC TGCAGGGGAT 
TGTGGCCTTC TGAAGGAGGG AGACATAATC CTGGCTGTCA ATGGAGAACC TGTCAGAGGC 
CTTTCCTATG AGGAGGTTCT GCATCTCCTC CAAAAATCTC CATCTGAGAT CAGACTGTCA 
ATCTGCAGAC CTTCACAGGA TGAGCATACA GATATTGAGA CCTTTCTGGT TCCAAACCGT 
GAACTGAGAT CCAGATCTCT CGACCTACAA GCGAGGTCTG TGGCTCCAGA TTTCAGTGAG 
CTCCTCTTGA GGCGGGCGAT AGAGATGGGG CTTAAAAAAG AAGCTTCACC TGAGGAAACC 
CAGGAGAGTG AGCTGCTGGA AACAGATAAA TCAATTCCTC CACCTCTACC TCCACGTCCA 
TCTTCTGCTG AAGGTGGAGA AAGAAAACCT CATGTCATTC TAGTGGAGGA GTATGAGAAG 
AAAGACCCGC CTCCTCCTCC GACTCCTCCT CCAACACCAG GCATTCTTAA AGCGTCAACA 
ACCAACACTG TGCAAACCCC AGCAGCACCT AAAACTGCAA TTAAAGCCCA TCAGCAAGAG 
TCACTCGAAC CAACTGTCTC TAGAAGCAAC CATGTCTCAG CATGTACGAG TCACGTGAAA 
GATGAAGGAG TGGAAGAGAA CGTGATAAGT TTCACAGCTA ATGGTCTGAC CGTCACTGCT 
GGTGAAGAAT ACCTGACCAT CAGCACTACG GCTCCATCTC CCCCGAGCGG CAGCACTGCA 
GTCTCCTCTG AAGCTCCTCC TCCTGTGCCT GCACTTTCTC CATCACTGCT GAACACACCT 
CCAACATACC AGCAGGCCAT TCAAGCAGCA TCTCCAGCAG AGCCTCCTGC CCAGCCCAGC 
AACAACGGCT GGGATGATGA TGACGAAGAG GATGATGAAG ATCCCAGAAG GGATGTGTTT 
AAGGAGTTTG AACTGACCGT CTCACTGACC AAATCCTGGT GTGGAAGCTT CGGTTTTACA 
ATAACCAGGA GTAAATTGGA CAACTGCTAT TATGTCCAGG ATGTCCTGGA TAATCCAGCC 
AAAGCAGATG GCAGACTGAG GGCAGGAGAC AGGCTTATCA TGGTGAATGG TCATAATGTG 
ACCAGCGTGA CAGATGACGT GGCAATAAGC ATCCTCAGGT GCTCCTCCAA AAGACTGCAC 
ATGGTGCTGG GCAGAGCTGT GCAAAATCTG CTCCCTCCAC CACCTCCTGA CACCCTCCAG 
GACATTATCA TATCCAAGAC TCCATCTGGA CAACTGGGTA TTAAGCTAAC AGGAGGGATT 
GGAAGCAAAC GGCAAGGAAT TTATGTGCAG GAGGTGGTGC TGAGCTCTCC TGCTAGTGAG 
GAGGGCAGCA TTCAGCCCAA TGACAAGATT GTTTACATAT GTGGGAGATG CACTCTGGGA 
ATGACGCTGG AGGATGCGGT AAAAGTGTGT GAGAGCTCCC CACGCAAAGT CAGATTCAAA 
GCCATGAGAG ATGATCAACC AGTCATACCA AGAGATAAGT GGAACGGTTT GTTTGAATGG 
AAAGAGAAGA AAGGTTTCCC TCACCTGGAG GAGCCCATGT CACCTGACGT GCATTCAGCT 
CTTGCAGACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307422.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305697.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.

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CloneID	ODa46180
Clone ID Related Accession (Same CDS sequence)	XM_009307422.3 , XM_009307422.2
Accession Version	XM_009307422.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGACGAAGC 
ACAAGTTTTT GGTCACGAAG TTCGTGTCTA AACTCTCCAA ACAAAGGTGA AGTCCGACCT 
TCCAGTCCCT GCATTACTGT GAGTGAGTCT GCAGTCAGTC TGTCCCAAAG AAAAGCTAAG 
AGTCTTGGAC CTGAGTTTAC CAGGATGAAC GATGAGCCTC AAATCATTTT AGAGCTGCCG 
GGGTCTATAG TGTCGAAGAA AGGCAAGTCA GGTCTGTCTC AGAGGGAGGT TAACGTGATC 
ATGCCAAATG AACAGTGTGT GGCAGTGAAA TGTGACATCA AGTCCCGAGG GAGGGATGTG 
TTCGACATGG TTGTGGCACA CGCTAACCTT GTGGAGCATT TTTACTTTGG CCTTGCTTTC 
ATTGATGATG GTGAATTTTT CTTCTTGGAC CCCGAGACAA AAATCTCAAA GGTTGCTCCT 
GATTACTGGA AGAAAGTGGC TTCTGCGACA TTTACACTTT ATCTTCGTGT CAGATTTTTC 
CCTGATGACA TCTCCTACAT TCTACATAAA CATACTCGAC ACCAATATTA TCTCCAGCTG 
AGGAAAGATA TTCTGGAAGA CAGAGTGCAC TGTAATGAGG AGACGGCGCT GTTTCTCGCT 
GCGCTGGCAC TGCAGGCTGA GTTTGGAGAT TATATGGCTG AAGTTTACGG GAAGAACTAT 
TTCCAGACGG AGCAGTACAT CTCTAAGAGA GTCATAGAGA AAGTGGCCTT GCCCTGTTTG 
AGAGAGGAAT TGCCAAGGTT ACATGCCAAT AATGCCCAAA TGCTTCCAGA GGAGGCAGAG 
ACGGAGTTCT TAAAGATTGC ACAGCAACTA CCAGAGTATG GGGTGCTGTT TCACAGAGTT 
GCCCGTGAAC GGAAACCTAT TATTGGAGAG CTGGTTTTGG GAATTTGTGC CAAAGGAATT 
CTGGTTTATG AAGTCAAGAA TAATTCTCGG ATGTTAAGCC GCAGATTTCA CTGGACAGAA 
ACTGACAGTC TCTCAACAAG TAGACGAAAA TTTACCATTG AGTGCAGCCC AAGTGGAAAG 
AAGCACTCGT TTGTGACGGA GAGCTCAAAA ATCGCACAGT ACCTCCTGAA CCTGTGCTCG 
GCCCAACACA AGTTTCACAG TGAGATGACC TCACGCCAGC TGACCTACTG CTTAGCTTCA 
GATGACAGCG TAGCAAAATA TACATCCATA TGCCGTGCTC GACACGAGCA GATAAAGAGG 
CTGTCCTGCT CAGAGATTGT GCTCAATAAT ATTGGTCTGA ATGGATTGCC AAGTGACTCT 
ATAAGCAAGT CTTGCGATGA CCTGTCGGCG AAAATTGGAG TTCGGATCCG GCAACAGAAT 
GAAGTAAATT ACAGTCCTGA ATCGAGAGCA CCCTGTGAAC CAAAAGATCA GAGCCAAAGC 
AGCAGCCCAC TGGAAACCTT CAGTCCCCCA GTCTATCGCA TCATGTCCAA TGTCTCTTTG 
CAAAAGCAGG ACTCAGAGGT TCATTCCACT ACCTCATCTA TAAGAGTGGA CACACCCACA 
AAAACAACCC CAGAGAGAGA AATCATCTGT GTCACTTTAA AGAAGGATCC CAAACTTGGC 
TATGGTTTCA TAATAGTCGG GGAGGACAAC ACAGGGAAGT TAGATCTTGG AATTTTTATT 
GCATCCATCA TTCCCGATGG TCCAGCTGAT AAGGATGGGA GAATTAGACC AGGTGGTCGG 
CTGATCTCTC TGAATAACAT CAGTCTAGAA GGGGTGACTT TTAATATAGC AGCTTCCATA 
CTCCAGAACA GCCCAGAAGA AGTGGAGCTT ATAGTGTCTC AACCCAAACA GGATCTTCGG 
GGCAGCAAGA CTTCACTGGC TCCAAGTAAT TTAGGAATGA TGCTAGAAAG AAGCTTTGGC 
TCCCAAAGCA CACTGAGCGC CGAGTACAGA CCTGTGATGG AGGAGCTGGA GGAGACCCTC 
TCCAACATCA TGGCCCCTAA AATCAACAAA CGGATGCACA TACCTGTGGT CCGCATTCTG 
GATATTCAGG ACGAGATGTC TAAAAGTTCT TCTGGCTTCA GTTTGAAACC AGGTGAAATA 
ATCTACATGG AGCTCCGAAA GATCAACGGC AGTTTGGGAA TCAGTGTTGC TGGTGGGATC 
AACACTAATG TACATCATGG AGGAATCTAC ATCAAAAGTG TGATAGCAGG AGGAGCGGCA 
GATCAAGATG GAAGAATACA GATAGGTGAC AGACTGCTGG AGGTGGACGG ATGTAACCTG 
CGTGCCGTCA CACACAGGCA GGCGGTGGAG TGCCTGAAGA GAACCGGAGA GGTGGTGAGT 
CTGGTTCTGG AGCGAGAGCC TCCGGTGGTG CTGGACACTT CCAGTGCCTC ATCCACATTG 
GAGAGACGAA GATCTCCTTC ACCCCTCAGT GCCCCCCTGA GGAGAGACGT GCCCCTACAA 
ACGCCCTTGT CTGTCAGGAC CAAGGACTTT AGATTTGTGT CAGATGAAAA TATCCTGGAA 
GTGACGTTGC AGAAGAGTTT CAGTGGCCTG GGCTTCAGTT TTCTCGTATC TGAGTTGGGT 
CCTGAAAAGG AAAACATTGT GCAGATCAAG AGTCTTTCCT CTGGTCATCC TGCAGGGGAT 
TGTGGCCTTC TGAAGGAGGG AGACATAATC CTGGCTGTCA ATGGAGAACC TGTCAGAGGC 
CTTTCCTATG AGGAGGTTCT GCATCTCCTC CAAAAATCTC CATCTGAGAT CAGACTGTCA 
ATCTGCAGAC CTTCACAGGA TGAGCATACA GATATTGAGA CCTTTCTGGT TCCAAACCGT 
GAACTGAGAT CCAGATCTCT CGACCTACAA GCGAGGTCTG TGGCTCCAGA TTTCAGTGAG 
CTCCTCTTGA GGCGGGCGAT AGAGATGGGG CTTAAAAAAG AAGCTTCACC TGAGGAAACC 
CAGGAGAGTG AGCTGCTGGA AACAGATAAA TCAATTCCTC CACCTCTACC TCCACGTCCA 
TCTTCTGCTG AAGGTGGAGA AAGAAAACCT CATGTCATTC TAGTGGAGGA GTATGAGAAG 
AAAGACCCGC CTCCTCCTCC GACTCCTCCT CCAACACCAG GCATTCTTAA AGCGTCAACA 
ACCAACACTG TGCAAACCCC AGCAGCACCT AAAACTGCAA TTAAAGCCCA TCAGCAAGAG 
TCACTCGAAC CAACTGTCTC TAGAAGCAAC CATGTCTCAG CATGTACGAG TCACGTGAAA 
GATGAAGGAG TGGAAGAGAA CGTGATAAGT TTCACAGCTA ATGGTCTGAC CGTCACTGCT 
GGTGAAGAAT ACCTGACCAT CAGCACTACG GCTCCATCTC CCCCGAGCGG CAGCACTGCA 
GTCTCCTCTG AAGCTCCTCC TCCTGTGCCT GCACTTTCTC CATCACTGCT GAACACACCT 
CCAACATACC AGCAGGCCAT TCAAGCAGCA TCTCCAGCAG AGCCTCCTGC CCAGCCCAGC 
AACAACGGCT GGGATGATGA TGACGAAGAG GATGATGAAG ATCCCAGAAG GGATGTGTTT 
AAGGAGTTTG AACTGACCGT CTCACTGACC AAATCCTGGT GTGGAAGCTT CGGTTTTACA 
ATAACCAGGA GTAAATTGGA CAACTGCTAT TATGTCCAGG ATGTCCTGGA TAATCCAGCC 
AAAGCAGATG GCAGACTGAG GGCAGGAGAC AGGCTTATCA TGGTGAATGG TCATAATGTG 
ACCAGCGTGA CAGATGACGT GGCAATAAGC ATCCTCAGGT GCTCCTCCAA AAGACTGCAC 
ATGGTGCTGG GCAGAGCTGT GCAAAATCTG CTCCCTCCAC CACCTCCTGA CACCCTCCAG 
GACATTATCA TATCCAAGAC TCCATCTGGA CAACTGGGTA TTAAGCTAAC AGGAGGGATT 
GGAAGCAAAC GGCAAGGAAT TTATGTGCAG GAGGTGGTGC TGAGCTCTCC TGCTAGTGAG 
GAGGGCAGCA TTCAGCCCAA TGACAAGATT GTTTACATAT GTGGGAGATG CACTCTGGGA 
ATGACGCTGG AGGATGCGGT AAAAGTGTGT GAGAGCTCCC CACGCAAAGT CAGATTCAAA 
GCCATGAGAG ATGATCAACC AGTCATACCA AGAGATAAGT GGAACGGTTT GTTTGAATGG 
AAAGAGAAGA AAGGTTTCCC TCACCTGGAG GAGCCCATGT CACCTGACGT GCATTCAGCT 
CTTGCAGACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305697.1
CDS	295..6858
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNGLFEWKEKKGFPHLEEPMSPDVHSALADVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307422.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307422.2

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGACGAAGC 
ACAAGTTTTT GGTCACGAAG TTCGTGTCTA AACTCTCCAA ACAAAGGTGA AGTCCGACCT 
TCCAGTCCCT GCATTACTGT GAGTGAGTCT GCAGTCAGTC TGTCCCAAAG AAAAGCTAAG 
AGTCTTGGAC CTGAGTTTAC CAGGATGAAC GATGAGCCTC AAATCATTTT AGAGCTGCCG 
GGGTCTATAG TGTCGAAGAA AGGCAAGTCA GGTCTGTCTC AGAGGGAGGT TAACGTGATC 
ATGCCAAATG AACAGTGTGT GGCAGTGAAA TGTGACATCA AGTCCCGAGG GAGGGATGTG 
TTCGACATGG TTGTGGCACA CGCTAACCTT GTGGAGCATT TTTACTTTGG CCTTGCTTTC 
ATTGATGATG GTGAATTTTT CTTCTTGGAC CCCGAGACAA AAATCTCAAA GGTTGCTCCT 
GATTACTGGA AGAAAGTGGC TTCTGCGACA TTTACACTTT ATCTTCGTGT CAGATTTTTC 
CCTGATGACA TCTCCTACAT TCTACATAAA CATACTCGAC ACCAATATTA TCTCCAGCTG 
AGGAAAGATA TTCTGGAAGA CAGAGTGCAC TGTAATGAGG AGACGGCGCT GTTTCTCGCT 
GCGCTGGCAC TGCAGGCTGA GTTTGGAGAT TATATGGCTG AAGTTTACGG GAAGAACTAT 
TTCCAGACGG AGCAGTACAT CTCTAAGAGA GTCATAGAGA AAGTGGCCTT GCCCTGTTTG 
AGAGAGGAAT TGCCAAGGTT ACATGCCAAT AATGCCCAAA TGCTTCCAGA GGAGGCAGAG 
ACGGAGTTCT TAAAGATTGC ACAGCAACTA CCAGAGTATG GGGTGCTGTT TCACAGAGTT 
GCCCGTGAAC GGAAACCTAT TATTGGAGAG CTGGTTTTGG GAATTTGTGC CAAAGGAATT 
CTGGTTTATG AAGTCAAGAA TAATTCTCGG ATGTTAAGCC GCAGATTTCA CTGGACAGAA 
ACTGACAGTC TCTCAACAAG TAGACGAAAA TTTACCATTG AGTGCAGCCC AAGTGGAAAG 
AAGCACTCGT TTGTGACGGA GAGCTCAAAA ATCGCACAGT ACCTCCTGAA CCTGTGCTCG 
GCCCAACACA AGTTTCACAG TGAGATGACC TCACGCCAGC TGACCTACTG CTTAGCTTCA 
GATGACAGCG TAGCAAAATA TACATCCATA TGCCGTGCTC GACACGAGCA GATAAAGAGG 
CTGTCCTGCT CAGAGATTGT GCTCAATAAT ATTGGTCTGA ATGGATTGCC AAGTGACTCT 
ATAAGCAAGT CTTGCGATGA CCTGTCGGCG AAAATTGGAG TTCGGATCCG GCAACAGAAT 
GAAGTAAATT ACAGTCCTGA ATCGAGAGCA CCCTGTGAAC CAAAAGATCA GAGCCAAAGC 
AGCAGCCCAC TGGAAACCTT CAGTCCCCCA GTCTATCGCA TCATGTCCAA TGTCTCTTTG 
CAAAAGCAGG ACTCAGAGGT TCATTCCACT ACCTCATCTA TAAGAGTGGA CACACCCACA 
AAAACAACCC CAGAGAGAGA AATCATCTGT GTCACTTTAA AGAAGGATCC CAAACTTGGC 
TATGGTTTCA TAATAGTCGG GGAGGACAAC ACAGGGAAGT TAGATCTTGG AATTTTTATT 
GCATCCATCA TTCCCGATGG TCCAGCTGAT AAGGATGGGA GAATTAGACC AGGTGGTCGG 
CTGATCTCTC TGAATAACAT CAGTCTAGAA GGGGTGACTT TTAATATAGC AGCTTCCATA 
CTCCAGAACA GCCCAGAAGA AGTGGAGCTT ATAGTGTCTC AACCCAAACA GGATCTTCGG 
GGCAGCAAGA CTTCACTGGC TCCAAGTAAT TTAGGAATGA TGCTAGAAAG AAGCTTTGGC 
TCCCAAAGCA CACTGAGCGC CGAGTACAGA CCTGTGATGG AGGAGCTGGA GGAGACCCTC 
TCCAACATCA TGGCCCCTAA AATCAACAAA CGGATGCACA TACCTGTGGT CCGCATTCTG 
GATATTCAGG ACGAGATGTC TAAAAGTTCT TCTGGCTTCA GTTTGAAACC AGGTGAAATA 
ATCTACATGG AGCTCCGAAA GATCAACGGC AGTTTGGGAA TCAGTGTTGC TGGTGGGATC 
AACACTAATG TACATCATGG AGGAATCTAC ATCAAAAGTG TGATAGCAGG AGGAGCGGCA 
GATCAAGATG GAAGAATACA GATAGGTGAC AGACTGCTGG AGGTGGACGG ATGTAACCTG 
CGTGCCGTCA CACACAGGCA GGCGGTGGAG TGCCTGAAGA GAACCGGAGA GGTGGTGAGT 
CTGGTTCTGG AGCGAGAGCC TCCGGTGGTG CTGGACACTT CCAGTGCCTC ATCCACATTG 
GAGAGACGAA GATCTCCTTC ACCCCTCAGT GCCCCCCTGA GGAGAGACGT GCCCCTACAA 
ACGCCCTTGT CTGTCAGGAC CAAGGACTTT AGATTTGTGT CAGATGAAAA TATCCTGGAA 
GTGACGTTGC AGAAGAGTTT CAGTGGCCTG GGCTTCAGTT TTCTCGTATC TGAGTTGGGT 
CCTGAAAAGG AAAACATTGT GCAGATCAAG AGTCTTTCCT CTGGTCATCC TGCAGGGGAT 
TGTGGCCTTC TGAAGGAGGG AGACATAATC CTGGCTGTCA ATGGAGAACC TGTCAGAGGC 
CTTTCCTATG AGGAGGTTCT GCATCTCCTC CAAAAATCTC CATCTGAGAT CAGACTGTCA 
ATCTGCAGAC CTTCACAGGA TGAGCATACA GATATTGAGA CCTTTCTGGT TCCAAACCGT 
GAACTGAGAT CCAGATCTCT CGACCTACAA GCGAGGTCTG TGGCTCCAGA TTTCAGTGAG 
CTCCTCTTGA GGCGGGCGAT AGAGATGGGG CTTAAAAAAG AAGCTTCACC TGAGGAAACC 
CAGGAGAGTG AGCTGCTGGA AACAGATAAA TCAATTCCTC CACCTCTACC TCCACGTCCA 
TCTTCTGCTG AAGGTGGAGA AAGAAAACCT CATGTCATTC TAGTGGAGGA GTATGAGAAG 
AAAGACCCGC CTCCTCCTCC GACTCCTCCT CCAACACCAG GCATTCTTAA AGCGTCAACA 
ACCAACACTG TGCAAACCCC AGCAGCACCT AAAACTGCAA TTAAAGCCCA TCAGCAAGAG 
TCACTCGAAC CAACTGTCTC TAGAAGCAAC CATGTCTCAG CATGTACGAG TCACGTGAAA 
GATGAAGGAG TGGAAGAGAA CGTGATAAGT TTCACAGCTA ATGGTCTGAC CGTCACTGCT 
GGTGAAGAAT ACCTGACCAT CAGCACTACG GCTCCATCTC CCCCGAGCGG CAGCACTGCA 
GTCTCCTCTG AAGCTCCTCC TCCTGTGCCT GCACTTTCTC CATCACTGCT GAACACACCT 
CCAACATACC AGCAGGCCAT TCAAGCAGCA TCTCCAGCAG AGCCTCCTGC CCAGCCCAGC 
AACAACGGCT GGGATGATGA TGACGAAGAG GATGATGAAG ATCCCAGAAG GGATGTGTTT 
AAGGAGTTTG AACTGACCGT CTCACTGACC AAATCCTGGT GTGGAAGCTT CGGTTTTACA 
ATAACCAGGA GTAAATTGGA CAACTGCTAT TATGTCCAGG ATGTCCTGGA TAATCCAGCC 
AAAGCAGATG GCAGACTGAG GGCAGGAGAC AGGCTTATCA TGGTGAATGG TCATAATGTG 
ACCAGCGTGA CAGATGACGT GGCAATAAGC ATCCTCAGGT GCTCCTCCAA AAGACTGCAC 
ATGGTGCTGG GCAGAGCTGT GCAAAATCTG CTCCCTCCAC CACCTCCTGA CACCCTCCAG 
GACATTATCA TATCCAAGAC TCCATCTGGA CAACTGGGTA TTAAGCTAAC AGGAGGGATT 
GGAAGCAAAC GGCAAGGAAT TTATGTGCAG GAGGTGGTGC TGAGCTCTCC TGCTAGTGAG 
GAGGGCAGCA TTCAGCCCAA TGACAAGATT GTTTACATAT GTGGGAGATG CACTCTGGGA 
ATGACGCTGG AGGATGCGGT AAAAGTGTGT GAGAGCTCCC CACGCAAAGT CAGATTCAAA 
GCCATGAGAG ATGATCAACC AGTCATACCA AGAGATAAGT GGAACGGTTT GTTTGAATGG 
AAAGAGAAGA AAGGTTTCCC TCACCTGGAG GAGCCCATGT CACCTGACGT GCATTCAGCT 
CTTGCAGACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307423.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305698.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.

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CloneID	ODa46181
Clone ID Related Accession (Same CDS sequence)	XM_009307423.2 , XM_009307423.3
Accession Version	XM_009307423.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305698.1
CDS	295..6798
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307423.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307423.2

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptpn20 ( XM_009307423.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305698.1 Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.

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CloneID	ODa46181
Clone ID Related Accession (Same CDS sequence)	XM_009307423.2 , XM_009307423.3
Accession Version	XM_009307423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tyrosine-protein phosphatase non-receptor type 13 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307423.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305698.1
CDS	306..6809
Translation	MSSTFVTLAEVLEARGGPLEEDEVWSLLLGSAESLVDLSYKGHNICNIITPASLLLSATGTLAFKNCAMTDEVCAFTAPEMLQGRTVSTKLAMEKIIVYSLGMTLYWSVDYHLPQNQPIQLSDSLNCLLLSMCEDMAHRRTNLNSILEVCESHHKASLLPPPNKVIKQLVEDVLQESVDRAALTENVVQLSGKSQMIRERLHGKRGPYSGYTEMNGAAGETRRLSLDSEPKQGIFHHQKTWSLKSRPSQTTSYQSSSRNPLGLHNRRSTSFWSRSSCLNSPNKGEVRPSSPCITVSESAVSLSQRKAKSLGPEFTRMNDEPQIILELPGSIVSKKGKSGLSQREVNVIMPNEQCVAVKCDIKSRGRDVFDMVVAHANLVEHFYFGLAFIDDGEFFFLDPETKISKVAPDYWKKVASATFTLYLRVRFFPDDISYILHKHTRHQYYLQLRKDILEDRVHCNEETALFLAALALQAEFGDYMAEVYGKNYFQTEQYISKRVIEKVALPCLREELPRLHANNAQMLPEEAETEFLKIAQQLPEYGVLFHRVARERKPIIGELVLGICAKGILVYEVKNNSRMLSRRFHWTETDSLSTSRRKFTIECSPSGKKHSFVTESSKIAQYLLNLCSAQHKFHSEMTSRQLTYCLASDDSVAKYTSICRARHEQIKRLSCSEIVLNNIGLNGLPSDSISKSCDDLSAKIGVRIRQQNEVNYSPESRAPCEPKDQSQSSSPLETFSPPVYRIMSNVSLQKQDSEVHSTTSSIRVDTPTKTTPEREIICVTLKKDPKLGYGFIIVGEDNTGKLDLGIFIASIIPDGPADKDGRIRPGGRLISLNNISLEGVTFNIAASILQNSPEEVELIVSQPKQDLRGSKTSLAPSNLGMMLERSFGSQSTLSAEYRPVMEELEETLSNIMAPKINKRMHIPVVRILDIQDEMSKSSSGFSLKPGEIIYMELRKINGSLGISVAGGINTNVHHGGIYIKSVIAGGAADQDGRIQIGDRLLEVDGCNLRAVTHRQAVECLKRTGEVVSLVLEREPPVVLDTSSASSTLERRRSPSPLSAPLRRDVPLQTPLSVRTKDFRFVSDENILEVTLQKSFSGLGFSFLVSELGPEKENIVQIKSLSSGHPAGDCGLLKEGDIILAVNGEPVRGLSYEEVLHLLQKSPSEIRLSICRPSQDEHTDIETFLVPNRELRSRSLDLQARSVAPDFSELLLRRAIEMGLKKEASPEETQESELLETDKSIPPPLPPRPSSAEGGERKPHVILVEEYEKKDPPPPPTPPPTPGILKASTTNTVQTPAAPKTAIKAHQQESLEPTVSRSNHVSACTSHVKDEGVEENVISFTANGLTVTAGEEYLTISTTAPSPPSGSTAVSSEAPPPVPALSPSLLNTPPTYQQAIQAASPAEPPAQPSNNGWDDDDEEDDEDPRRDVFKEFELTVSLTKSWCGSFGFTITRSKLDNCYYVQDVLDNPAKADGRLRAGDRLIMVNGHNVTSVTDDVAISILRCSSKRLHMVLGRAVQNLLPPPPPDTLQDIIISKTPSGQLGIKLTGGIGSKRQGIYVQEVVLSSPASEEGSIQPNDKIVYICGRCTLGMTLEDAVKVCESSPRKVRFKAMRDDQPVIPRDKWNVKAVADSAMLFRKRLSVTAEQESCILQVEFTKPDRGGLGFALVGGVNGSTLRVKDICCGGVAEQDGRLRVGDILLEVNGIIVSGLSHSKVVDILRKAEGTVQLTVCRDILPMSACSASNYPTEAHGNFNTLNSAAQTEAHPDHVSFHSDCPECEQESRNCTPTCQRCCPSLGVTDMLQEKTDPQKQHLENSAMKTEHSQSDGWSSDEEEDGDDDDCASQPSPPETIPLTGRPIVSEEELSSLAVISPPKNGQYTGSRVKALIQALEQLEKQDLVKEFMALEHLNPSDNCLVGKAPENREKNRYREILPYDKTRVPVGEDEGYINASYIRMKVGSEELFFISAQGPLPGTQDSFWQMVWENRSDVIAMMTREVERGRVKCHKYWPEKLDVPKETSRYQLFLDNYQMLGYFHIKVIKMVEKKSGDVHFVKHLKFTTWPDHGTPHSSDQLVRFIRYMRAVHAKGPITVHCSAGIGRAGVLICTDVILSLIQKDLSINVSDIVKEMRLQRHGMIQTKEQYLFCYKVWLEVLQNISLLYGHHWQTETSS

Target ORF information:

RefSeq Version	XM_009307423.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, non-receptor type 20 (ptpn20), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307423.3

ORF Insert Sequence:

ATGAGCAGCA CGTTTGTGAC CCTCGCCGAG GTGCTGGAGG CCAGAGGAGG ACCGCTGGAG 
GAGGACGAGG TCTGGTCCCT CCTGCTGGGC TCCGCTGAAT CACTCGTAGA TCTTTCCTAC 
AAAGGTCACA ATATCTGCAA CATTATAACT CCTGCATCAC TGCTGCTGTC CGCCACTGGG 
ACACTGGCGT TTAAGAACTG TGCAATGACA GATGAGGTGT GTGCCTTCAC CGCCCCGGAG 
ATGCTGCAAG GCCGTACTGT CTCCACCAAG CTCGCCATGG AGAAGATAAT CGTGTATTCT 
CTTGGTATGA CTCTGTACTG GTCAGTTGAT TATCATCTCC CTCAAAACCA GCCCATTCAG 
CTCAGTGACT CTCTGAATTG CCTTCTGCTG AGCATGTGTG AGGATATGGC CCATCGGCGA 
ACTAACCTCA ACTCCATCCT GGAGGTCTGC GAATCACACC ACAAAGCATC TCTCCTGCCT 
CCTCCTAATA AAGTCATCAA ACAGCTGGTG GAGGATGTCC TTCAAGAATC TGTGGATCGA 
GCAGCTTTAA CTGAAAATGT TGTGCAGTTG AGTGGGAAAA GTCAAATGAT CAGAGAAAGA 
CTTCATGGTA AACGTGGACC ATATTCTGGT TATACGGAGA TGAACGGAGC TGCAGGAGAG 
ACTCGCAGAC TGTCTCTGGA CTCTGAGCCA AAGCAAGGAA TATTTCATCA CCAGAAAACC 
TGGTCTCTCA AAAGCAGACC TAGTCAGACC ACATCCTACC AGAGCTCATC TAGAAATCCT 
CTGGGTCTTC ACAACAGACG AAGCACAAGT TTTTGGTCAC GAAGTTCGTG TCTAAACTCT 
CCAAACAAAG GTGAAGTCCG ACCTTCCAGT CCCTGCATTA CTGTGAGTGA GTCTGCAGTC 
AGTCTGTCCC AAAGAAAAGC TAAGAGTCTT GGACCTGAGT TTACCAGGAT GAACGATGAG 
CCTCAAATCA TTTTAGAGCT GCCGGGGTCT ATAGTGTCGA AGAAAGGCAA GTCAGGTCTG 
TCTCAGAGGG AGGTTAACGT GATCATGCCA AATGAACAGT GTGTGGCAGT GAAATGTGAC 
ATCAAGTCCC GAGGGAGGGA TGTGTTCGAC ATGGTTGTGG CACACGCTAA CCTTGTGGAG 
CATTTTTACT TTGGCCTTGC TTTCATTGAT GATGGTGAAT TTTTCTTCTT GGACCCCGAG 
ACAAAAATCT CAAAGGTTGC TCCTGATTAC TGGAAGAAAG TGGCTTCTGC GACATTTACA 
CTTTATCTTC GTGTCAGATT TTTCCCTGAT GACATCTCCT ACATTCTACA TAAACATACT 
CGACACCAAT ATTATCTCCA GCTGAGGAAA GATATTCTGG AAGACAGAGT GCACTGTAAT 
GAGGAGACGG CGCTGTTTCT CGCTGCGCTG GCACTGCAGG CTGAGTTTGG AGATTATATG 
GCTGAAGTTT ACGGGAAGAA CTATTTCCAG ACGGAGCAGT ACATCTCTAA GAGAGTCATA 
GAGAAAGTGG CCTTGCCCTG TTTGAGAGAG GAATTGCCAA GGTTACATGC CAATAATGCC 
CAAATGCTTC CAGAGGAGGC AGAGACGGAG TTCTTAAAGA TTGCACAGCA ACTACCAGAG 
TATGGGGTGC TGTTTCACAG AGTTGCCCGT GAACGGAAAC CTATTATTGG AGAGCTGGTT 
TTGGGAATTT GTGCCAAAGG AATTCTGGTT TATGAAGTCA AGAATAATTC TCGGATGTTA 
AGCCGCAGAT TTCACTGGAC AGAAACTGAC AGTCTCTCAA CAAGTAGACG AAAATTTACC 
ATTGAGTGCA GCCCAAGTGG AAAGAAGCAC TCGTTTGTGA CGGAGAGCTC AAAAATCGCA 
CAGTACCTCC TGAACCTGTG CTCGGCCCAA CACAAGTTTC ACAGTGAGAT GACCTCACGC 
CAGCTGACCT ACTGCTTAGC TTCAGATGAC AGCGTAGCAA AATATACATC CATATGCCGT 
GCTCGACACG AGCAGATAAA GAGGCTGTCC TGCTCAGAGA TTGTGCTCAA TAATATTGGT 
CTGAATGGAT TGCCAAGTGA CTCTATAAGC AAGTCTTGCG ATGACCTGTC GGCGAAAATT 
GGAGTTCGGA TCCGGCAACA GAATGAAGTA AATTACAGTC CTGAATCGAG AGCACCCTGT 
GAACCAAAAG ATCAGAGCCA AAGCAGCAGC CCACTGGAAA CCTTCAGTCC CCCAGTCTAT 
CGCATCATGT CCAATGTCTC TTTGCAAAAG CAGGACTCAG AGGTTCATTC CACTACCTCA 
TCTATAAGAG TGGACACACC CACAAAAACA ACCCCAGAGA GAGAAATCAT CTGTGTCACT 
TTAAAGAAGG ATCCCAAACT TGGCTATGGT TTCATAATAG TCGGGGAGGA CAACACAGGG 
AAGTTAGATC TTGGAATTTT TATTGCATCC ATCATTCCCG ATGGTCCAGC TGATAAGGAT 
GGGAGAATTA GACCAGGTGG TCGGCTGATC TCTCTGAATA ACATCAGTCT AGAAGGGGTG 
ACTTTTAATA TAGCAGCTTC CATACTCCAG AACAGCCCAG AAGAAGTGGA GCTTATAGTG 
TCTCAACCCA AACAGGATCT TCGGGGCAGC AAGACTTCAC TGGCTCCAAG TAATTTAGGA 
ATGATGCTAG AAAGAAGCTT TGGCTCCCAA AGCACACTGA GCGCCGAGTA CAGACCTGTG 
ATGGAGGAGC TGGAGGAGAC CCTCTCCAAC ATCATGGCCC CTAAAATCAA CAAACGGATG 
CACATACCTG TGGTCCGCAT TCTGGATATT CAGGACGAGA TGTCTAAAAG TTCTTCTGGC 
TTCAGTTTGA AACCAGGTGA AATAATCTAC ATGGAGCTCC GAAAGATCAA CGGCAGTTTG 
GGAATCAGTG TTGCTGGTGG GATCAACACT AATGTACATC ATGGAGGAAT CTACATCAAA 
AGTGTGATAG CAGGAGGAGC GGCAGATCAA GATGGAAGAA TACAGATAGG TGACAGACTG 
CTGGAGGTGG ACGGATGTAA CCTGCGTGCC GTCACACACA GGCAGGCGGT GGAGTGCCTG 
AAGAGAACCG GAGAGGTGGT GAGTCTGGTT CTGGAGCGAG AGCCTCCGGT GGTGCTGGAC 
ACTTCCAGTG CCTCATCCAC ATTGGAGAGA CGAAGATCTC CTTCACCCCT CAGTGCCCCC 
CTGAGGAGAG ACGTGCCCCT ACAAACGCCC TTGTCTGTCA GGACCAAGGA CTTTAGATTT 
GTGTCAGATG AAAATATCCT GGAAGTGACG TTGCAGAAGA GTTTCAGTGG CCTGGGCTTC 
AGTTTTCTCG TATCTGAGTT GGGTCCTGAA AAGGAAAACA TTGTGCAGAT CAAGAGTCTT 
TCCTCTGGTC ATCCTGCAGG GGATTGTGGC CTTCTGAAGG AGGGAGACAT AATCCTGGCT 
GTCAATGGAG AACCTGTCAG AGGCCTTTCC TATGAGGAGG TTCTGCATCT CCTCCAAAAA 
TCTCCATCTG AGATCAGACT GTCAATCTGC AGACCTTCAC AGGATGAGCA TACAGATATT 
GAGACCTTTC TGGTTCCAAA CCGTGAACTG AGATCCAGAT CTCTCGACCT ACAAGCGAGG 
TCTGTGGCTC CAGATTTCAG TGAGCTCCTC TTGAGGCGGG CGATAGAGAT GGGGCTTAAA 
AAAGAAGCTT CACCTGAGGA AACCCAGGAG AGTGAGCTGC TGGAAACAGA TAAATCAATT 
CCTCCACCTC TACCTCCACG TCCATCTTCT GCTGAAGGTG GAGAAAGAAA ACCTCATGTC 
ATTCTAGTGG AGGAGTATGA GAAGAAAGAC CCGCCTCCTC CTCCGACTCC TCCTCCAACA 
CCAGGCATTC TTAAAGCGTC AACAACCAAC ACTGTGCAAA CCCCAGCAGC ACCTAAAACT 
GCAATTAAAG CCCATCAGCA AGAGTCACTC GAACCAACTG TCTCTAGAAG CAACCATGTC 
TCAGCATGTA CGAGTCACGT GAAAGATGAA GGAGTGGAAG AGAACGTGAT AAGTTTCACA 
GCTAATGGTC TGACCGTCAC TGCTGGTGAA GAATACCTGA CCATCAGCAC TACGGCTCCA 
TCTCCCCCGA GCGGCAGCAC TGCAGTCTCC TCTGAAGCTC CTCCTCCTGT GCCTGCACTT 
TCTCCATCAC TGCTGAACAC ACCTCCAACA TACCAGCAGG CCATTCAAGC AGCATCTCCA 
GCAGAGCCTC CTGCCCAGCC CAGCAACAAC GGCTGGGATG ATGATGACGA AGAGGATGAT 
GAAGATCCCA GAAGGGATGT GTTTAAGGAG TTTGAACTGA CCGTCTCACT GACCAAATCC 
TGGTGTGGAA GCTTCGGTTT TACAATAACC AGGAGTAAAT TGGACAACTG CTATTATGTC 
CAGGATGTCC TGGATAATCC AGCCAAAGCA GATGGCAGAC TGAGGGCAGG AGACAGGCTT 
ATCATGGTGA ATGGTCATAA TGTGACCAGC GTGACAGATG ACGTGGCAAT AAGCATCCTC 
AGGTGCTCCT CCAAAAGACT GCACATGGTG CTGGGCAGAG CTGTGCAAAA TCTGCTCCCT 
CCACCACCTC CTGACACCCT CCAGGACATT ATCATATCCA AGACTCCATC TGGACAACTG 
GGTATTAAGC TAACAGGAGG GATTGGAAGC AAACGGCAAG GAATTTATGT GCAGGAGGTG 
GTGCTGAGCT CTCCTGCTAG TGAGGAGGGC AGCATTCAGC CCAATGACAA GATTGTTTAC 
ATATGTGGGA GATGCACTCT GGGAATGACG CTGGAGGATG CGGTAAAAGT GTGTGAGAGC 
TCCCCACGCA AAGTCAGATT CAAAGCCATG AGAGATGATC AACCAGTCAT ACCAAGAGAT 
AAGTGGAACG TTAAAGCAGT CGCTGACAGT GCCATGCTGT TCAGGAAGAG GCTGTCGGTC 
ACAGCGGAGC AGGAGAGCTG TATCCTGCAG GTGGAGTTCA CCAAGCCTGA TCGTGGTGGG 
CTGGGCTTTG CACTGGTGGG CGGAGTCAAC GGCAGCACAC TGAGAGTGAA GGACATATGC 
TGTGGGGGCG TGGCTGAGCA GGACGGTCGT CTTCGAGTGG GTGACATCCT TCTGGAGGTT 
AATGGCATCA TTGTATCAGG ACTGAGCCAT AGTAAAGTTG TGGATATTTT GAGGAAGGCG 
GAGGGCACGG TTCAGCTGAC GGTGTGTAGG GACATCCTGC CCATGTCCGC CTGCTCTGCT 
TCCAACTACC CCACTGAGGC CCATGGGAAT TTCAACACAC TGAATTCTGC AGCACAAACT 
GAAGCACATC CAGATCACGT GTCTTTCCAC TCAGACTGTC CTGAATGTGA GCAGGAAAGT 
CGAAACTGTA CACCTACCTG TCAGAGGTGT TGTCCGTCTC TGGGAGTCAC AGACATGCTA 
CAAGAAAAGA CAGACCCCCA GAAGCAGCAT CTAGAAAATT CTGCCATGAA AACAGAGCAC 
AGCCAGTCGG ACGGCTGGAG CAGTGACGAG GAAGAAGATG GTGACGATGA CGACTGTGCT 
TCCCAGCCTT CGCCTCCAGA GACTATACCT CTGACAGGTC GGCCAATCGT GTCAGAAGAG 
GAGCTTTCCA GTCTTGCAGT GATTTCTCCT CCTAAGAATG GGCAGTACAC CGGCTCCAGG 
GTCAAAGCCC TCATCCAGGC CCTCGAGCAG CTAGAAAAGC AAGATCTGGT CAAAGAGTTT 
ATGGCTTTGG AGCATTTAAA CCCTTCAGAC AACTGCCTGG TTGGAAAAGC ACCTGAAAAC 
CGGGAAAAGA ACAGATACAG GGAAATCCTG CCTTACGATA AGACGCGTGT TCCTGTGGGA 
GAGGACGAGG GCTACATCAA TGCCAGCTAC ATTCGTATGA AGGTGGGCTC GGAGGAGCTG 
TTCTTCATCT CCGCTCAGGG CCCTTTGCCT GGCACTCAGG ACAGTTTCTG GCAGATGGTG 
TGGGAGAACA GGTCTGATGT GATCGCCATG ATGACTCGAG AGGTGGAGCG CGGACGGGTC 
AAATGCCACA AATACTGGCC CGAGAAGCTC GACGTCCCCA AAGAGACCAG CCGTTACCAG 
CTCTTCCTGG ACAACTACCA GATGCTTGGT TATTTCCACA TTAAGGTCAT CAAGATGGTG 
GAAAAAAAGA GTGGAGACGT GCACTTTGTG AAGCACCTGA AGTTCACCAC ATGGCCTGAT 
CACGGCACAC CCCACTCCTC CGATCAGCTT GTGCGCTTCA TCCGCTACAT GAGGGCTGTT 
CACGCTAAAG GGCCCATCAC CGTCCACTGC AGCGCAGGCA TCGGCCGAGC CGGAGTCCTC 
ATCTGTACAG ACGTCATCCT CAGTCTCATT CAGAAAGACC TCAGTATTAA TGTGAGTGAC 
ATTGTAAAGG AGATGAGACT CCAGCGGCAC GGCATGATTC AGACTAAGGA ACAATACCTC 
TTTTGTTATA AAGTCTGGTT GGAAGTGTTA CAAAACATCT CGCTCCTTTA TGGTCACCAC 
TGGCAAACCG AAACCTCTTC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ptpn20
Entrez Gene ID	571774
Full Name	protein tyrosine phosphatase, non-receptor type 20
Synonyms	si:ch211-223a10.7
General protein information	Preferred Names protein tyrosine phosphatase, non-receptor type 20 Names FERM and PDZ domain-containing protein 2 tyrosine-protein phosphatase non-receptor type 13 tyrosine-protein phosphatase non-receptor type 20
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Gallus gallus (chicken)	FRMPD2	XP_421649.4
Danio rerio (zebrafish)	ptpn20	XP_002664234.3
Xenopus tropicalis (tropical clawed frog)	LOC100491969	XP_002940375.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ptpn20 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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