SI:CH211-167B20.8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-167B20.8
Entrez Gene ID	571155
Full Name	si:ch211-167b20.8
General protein information	Preferred Names si:ch211-167b20.8 Names protein phosphatase 1 regulatory subunit 3A-like uncharacterized protein si:ch211-167b20.8
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167121.3	XP_005167178.1	uncharacterized protein si:ch211-167b20.8 isoform X2
XM_003199091.4	XP_003199139.1	uncharacterized protein si:ch211-167b20.8 isoform X1
XM_003199091.5	XP_003199139.1	uncharacterized protein si:ch211-167b20.8 isoform X1
XM_021478446.1	XP_021334121.1	uncharacterized protein si:ch211-167b20.8 isoform X2
XM_005167122.4	XP_005167179.1	protein phosphatase 1 regulatory subunit 3A-like isoform X3
XM_005167122.3	XP_005167179.1	protein phosphatase 1 regulatory subunit 3A-like isoform X3

Pathways from BioSystems
Gene Ontology

The following SI:CH211-167B20.8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-167B20.8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa14324	XM_005167122.4 Latest version!	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa14322	XM_003199091.5 Latest version!	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa14323	XM_021478446.1 Latest version!	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa14323	XM_005167121.3 Latest version!	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
ODa14322	XM_003199091.4	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa14324	XM_005167122.3	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-167B20.8 ( XM_005167122.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167179.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa14324
Clone ID Related Accession (Same CDS sequence)	XM_005167122.4 , XM_005167122.3
Accession Version	XM_005167122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein phosphatase 1 regulatory subunit 3A-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167122.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGC TCATTGAAGC TTCGGCATCA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167179.1
CDS	260..1255
Translation	MAQGNNNNFLSIPSQQGLFKTVRVERSEDNSNDEDEEETEEDVRLIPRSSPVPRKRGSSIADETAEYMRIRLALTDRRVSFVDSMGAELADVRMFVPFDSDEEDNSRWEEEEAKYRKAYSEPTYRLWPEFQVLTGPELLLAVHTNKLEVDSVTPVPDEPLSFDVVIRVLNISFHKSVYVRSTMDGWINHFDYPAEYVQDSNDGETDKFSVKLSFAPPYLFNGARIDFVVRYETSDGEFWANNSGRNYSVTLLQSYDEDSVQTCAAEKPELRGILKPPRYRTASGYDDSDDREDGNLSNDECEAPENQDYFAQPTIVQPEIDIELIEASAST

Target ORF information:

RefSeq Version	XM_005167122.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167122.4

ORF Insert Sequence:

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGC TCATTGAAGC TTCGGCATCA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-167B20.8 ( XM_005167122.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167179.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa14324
Clone ID Related Accession (Same CDS sequence)	XM_005167122.4 , XM_005167122.3
Accession Version	XM_005167122.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein phosphatase 1 regulatory subunit 3A-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167122.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGC TCATTGAAGC TTCGGCATCA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167179.1
CDS	367..1362
Translation	MAQGNNNNFLSIPSQQGLFKTVRVERSEDNSNDEDEEETEEDVRLIPRSSPVPRKRGSSIADETAEYMRIRLALTDRRVSFVDSMGAELADVRMFVPFDSDEEDNSRWEEEEAKYRKAYSEPTYRLWPEFQVLTGPELLLAVHTNKLEVDSVTPVPDEPLSFDVVIRVLNISFHKSVYVRSTMDGWINHFDYPAEYVQDSNDGETDKFSVKLSFAPPYLFNGARIDFVVRYETSDGEFWANNSGRNYSVTLLQSYDEDSVQTCAAEKPELRGILKPPRYRTASGYDDSDDREDGNLSNDECEAPENQDYFAQPTIVQPEIDIELIEASAST

Target ORF information:

RefSeq Version	XM_005167122.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167122.3

ORF Insert Sequence:

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGC TCATTGAAGC TTCGGCATCA ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-167B20.8 ( XM_003199091.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003199139.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14322
Clone ID Related Accession (Same CDS sequence)	XM_003199091.4 , XM_003199091.5
Accession Version	XM_003199091.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-167b20.8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003199091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGA CTGCCAAAAA TCTGTCCAGT TCCCCAGAGT CCACCAAAAC TTCCTCCATG 
GCAGGATGTT CTCTTTCCAC TTCAGATATT CCCTACGGAG AAACACTTCC ACTAGAGTCA 
ATGTCATCCA TAAATTCTCC CCAAATAGAA GAGTCTGGCC TTCATCCATC AATTTTATCC 
ATCCCCCAAT CACAGTCAAC CCAGGAGTCC TCAGAGGCAC AAGATCCAGT CTATAATGTT 
CTCCCTTCTG TGTCTTTTTT GGTTCCTGCA GTCCTTGTCC ACTCCTATGA TAAACAAGAG 
GCTAGCAATA ATGAATCTGA GGTGTCCAGC AACCCCAACA CACTGTCTCA TCCACAGAAT 
GGGATGTTAG CGCAAGTGCC TTTCGAGTTG GGCCAACCTT TACTGGCTGC GTTTCCAGAC 
ACGACACATA CCGAGAAAGA AGATCTGGAG GAATTTCAGG AGAAGAAGGA TGTTGTGGAA 
ATGTATGACA AACTGTCAGA CATTCAAGAA GAACCAAGTC AGAATGTAGA AGGAGATAAA 
ACTTGCATTC AGACGTCGGA AAATCCCACA CAGGCCTCTT TCTCAAAAAA GGTAAAAGTT 
GCAACTGAAG ATGTAGAGAA GCTGATTTGG TCAGAGGGCT TGGAGAATCC ACTGAATGAA 
GGAGGTCTAA AAGATTTTCA GGAAAAGGAA GATGTGAAAA TAGACAACAG ATTGGCAAAC 
AGTCAAGAAG AATCAAGTCA GAATATAGAA GAAGATAAAA CTTACATTCA CAGGTCAGAA 
TTTCTTAAAC AGCTTCCTTC CTCAACACAG TTAAACCTTG CAAATGTAGA TTTAGAAAAC 
GTCAATTTGT CAGAGTTTTT AGAGAACCGT CTAGATGAAA ATCCCAAGCA GTCTTCTTCC 
TCAATACAGG TAAATGTTGC AACTGAAGAT GTAGAGAAGC TTAGTTTGTC AGATGGGTTA 
GACAGTTCTC TGGAGAACAG TCTGAAGCAT CTAAAGGAAA ATGAAGATGT TGTGGAAATT 
GTTAACAGCT TGGCAGATAT TCAAGAAGAA CCAAGCCAAA GTATAGAAGA AGATAAAACT 
TACAATTACA AGTCAGAATC TCCCAAACAG CCTTGCTCCT CAATACAGGT AAATGTTGCA 
ACTGAAGATG AAAAGCTCAC TTTGTCGGAT GGGTTAGAGA ATCCTCTGAA AGAAGGTCTG 
AAGGATTTTC AGGAAAATGA AGATGTTGTG AAAACGGTTG ACAGTTTGGC AGATGTTCTA 
GAAGAACCAA GTCAGAATAT AGAAGAAGAT AAAATGTACA GTCACGGCTC GGAAGATCCC 
AAGTCACTTC CTTCCTCAAA AGAGGTAAAC ATTGTTACCG AAGACACAGC GAAACACAAT 
TTGTCAGAAG AAATAGTGAT TCCAGTGTAT GAAAAGGATC TAAAAGATTT TCAGGAAAAT 
GAAGATGTTG TGAAAACTGA TGAAAGATCG GCAGACATTC TTGAAGAACC AAGTCAGAAT 
ACAGAAGATG AAATGTACAG TCACAGGTCG AAAAATCCCA AGTCGCTTCC TTCCTCAATA 
GAGGTACACA TTGAAACTGA AGATGTTGAG AAGCTGATTT TGTCAGAAGG CTTAAAGAAT 
GTTCTGGATG AAAAATGTCT AGACGATTTT CAGGAAAAGG AAGACGCTGT GAAAACTGAC 
AAAATATTGG CAGATATTCT TGAAGAGCCA AATCAGGATC TAGAAAATAA CACTTGTGAT 
CAGCTGCTTG AAGAACCCAC GCTGCCTCCT TCCTCTAAGG AGGTAATCAC TGCAACAGAA 
GATGCAGAGA AGCTTTCAGA TAGCTTGGAG CATCCTCTGG AAGAAGGAAT TAAAGAAGTA 
GATCTTGTCT CTTTGATTGG AATTAAGCGT GCCCAGGTGT TCGAAGTGGA AAGCACCAAA 
ACAGGGGATG AGGAATATGA TGCTTTGGTA GCTTCCTATT TAGATACCAC TTTACAGGAC 
AGAGAAACCC AAAGTTTACT AATCAATCCA ACCAGTCAAG AAGAAGGACT CTTGGATCCA 
AAAACCCTGG CCTCAATCCT GCCTGAGGAC CCAAACATTA AAAACCAAAC TGTTTCTATG 
TGTACTTCAG AGCATCCAAC AAGCCAACCA GAAAAGCCAG CACTGGTCAG TGGTTCTGAA 
ACATCACCAA GAGCCTTCGA TCATCAGCCT TCAACTGTTC AAACCTCAGC AGAAGAAGAT 
TTAACGTCTG AGCCTGATGA ACTTTCCCAA GAGGCATGTA AAGACCTTGA ACCTCAGCAC 
ACACTGCCAA GAGTCGACAG TGAACTATCT CAAATATCAA AGGATCAGGC TGAGGTCTCT 
CTGAGCACGT GTCTCATGGT CTCCGTAACC TTCTTCACTG CTGTTATCTG CCTGGCTGTA 
GGGATTCAAG AGCCCAGCGC TTTTCTGTGT GTGGGATTAT TCCTGTTGTC CCTTTGGTTC 
TTTGGTTCTC CCATCCACTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199139.1
CDS	367..3813
Translation	MAQGNNNNFLSIPSQQGLFKTVRVERSEDNSNDEDEEETEEDVRLIPRSSPVPRKRGSSIADETAEYMRIRLALTDRRVSFVDSMGAELADVRMFVPFDSDEEDNSRWEEEEAKYRKAYSEPTYRLWPEFQVLTGPELLLAVHTNKLEVDSVTPVPDEPLSFDVVIRVLNISFHKSVYVRSTMDGWINHFDYPAEYVQDSNDGETDKFSVKLSFAPPYLFNGARIDFVVRYETSDGEFWANNSGRNYSVTLLQSYDEDSVQTCAAEKPELRGILKPPRYRTASGYDDSDDREDGNLSNDECEAPENQDYFAQPTIVQPEIDIETAKNLSSSPESTKTSSMAGCSLSTSDIPYGETLPLESMSSINSPQIEESGLHPSILSIPQSQSTQESSEAQDPVYNVLPSVSFLVPAVLVHSYDKQEASNNESEVSSNPNTLSHPQNGMLAQVPFELGQPLLAAFPDTTHTEKEDLEEFQEKKDVVEMYDKLSDIQEEPSQNVEGDKTCIQTSENPTQASFSKKVKVATEDVEKLIWSEGLENPLNEGGLKDFQEKEDVKIDNRLANSQEESSQNIEEDKTYIHRSEFLKQLPSSTQLNLANVDLENVNLSEFLENRLDENPKQSSSSIQVNVATEDVEKLSLSDGLDSSLENSLKHLKENEDVVEIVNSLADIQEEPSQSIEEDKTYNYKSESPKQPCSSIQVNVATEDEKLTLSDGLENPLKEGLKDFQENEDVVKTVDSLADVLEEPSQNIEEDKMYSHGSEDPKSLPSSKEVNIVTEDTAKHNLSEEIVIPVYEKDLKDFQENEDVVKTDERSADILEEPSQNTEDEMYSHRSKNPKSLPSSIEVHIETEDVEKLILSEGLKNVLDEKCLDDFQEKEDAVKTDKILADILEEPNQDLENNTCDQLLEEPTLPPSSKEVITATEDAEKLSDSLEHPLEEGIKEVDLVSLIGIKRAQVFEVESTKTGDEEYDALVASYLDTTLQDRETQSLLINPTSQEEGLLDPKTLASILPEDPNIKNQTVSMCTSEHPTSQPEKPALVSGSETSPRAFDHQPSTVQTSAEEDLTSEPDELSQEACKDLEPQHTLPRVDSELSQISKDQAEVSLSTCLMVSVTFFTAVICLAVGIQEPSAFLCVGLFLLSLWFFGSPIHFS

Target ORF information:

RefSeq Version	XM_003199091.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199091.4

ORF Insert Sequence:

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGA CTGCCAAAAA TCTGTCCAGT TCCCCAGAGT CCACCAAAAC TTCCTCCATG 
GCAGGATGTT CTCTTTCCAC TTCAGATATT CCCTACGGAG AAACACTTCC ACTAGAGTCA 
ATGTCATCCA TAAATTCTCC CCAAATAGAA GAGTCTGGCC TTCATCCATC AATTTTATCC 
ATCCCCCAAT CACAGTCAAC CCAGGAGTCC TCAGAGGCAC AAGATCCAGT CTATAATGTT 
CTCCCTTCTG TGTCTTTTTT GGTTCCTGCA GTCCTTGTCC ACTCCTATGA TAAACAAGAG 
GCTAGCAATA ATGAATCTGA GGTGTCCAGC AACCCCAACA CACTGTCTCA TCCACAGAAT 
GGGATGTTAG CGCAAGTGCC TTTCGAGTTG GGCCAACCTT TACTGGCTGC GTTTCCAGAC 
ACGACACATA CCGAGAAAGA AGATCTGGAG GAATTTCAGG AGAAGAAGGA TGTTGTGGAA 
ATGTATGACA AACTGTCAGA CATTCAAGAA GAACCAAGTC AGAATGTAGA AGGAGATAAA 
ACTTGCATTC AGACGTCGGA AAATCCCACA CAGGCCTCTT TCTCAAAAAA GGTAAAAGTT 
GCAACTGAAG ATGTAGAGAA GCTGATTTGG TCAGAGGGCT TGGAGAATCC ACTGAATGAA 
GGAGGTCTAA AAGATTTTCA GGAAAAGGAA GATGTGAAAA TAGACAACAG ATTGGCAAAC 
AGTCAAGAAG AATCAAGTCA GAATATAGAA GAAGATAAAA CTTACATTCA CAGGTCAGAA 
TTTCTTAAAC AGCTTCCTTC CTCAACACAG TTAAACCTTG CAAATGTAGA TTTAGAAAAC 
GTCAATTTGT CAGAGTTTTT AGAGAACCGT CTAGATGAAA ATCCCAAGCA GTCTTCTTCC 
TCAATACAGG TAAATGTTGC AACTGAAGAT GTAGAGAAGC TTAGTTTGTC AGATGGGTTA 
GACAGTTCTC TGGAGAACAG TCTGAAGCAT CTAAAGGAAA ATGAAGATGT TGTGGAAATT 
GTTAACAGCT TGGCAGATAT TCAAGAAGAA CCAAGCCAAA GTATAGAAGA AGATAAAACT 
TACAATTACA AGTCAGAATC TCCCAAACAG CCTTGCTCCT CAATACAGGT AAATGTTGCA 
ACTGAAGATG AAAAGCTCAC TTTGTCGGAT GGGTTAGAGA ATCCTCTGAA AGAAGGTCTG 
AAGGATTTTC AGGAAAATGA AGATGTTGTG AAAACGGTTG ACAGTTTGGC AGATGTTCTA 
GAAGAACCAA GTCAGAATAT AGAAGAAGAT AAAATGTACA GTCACGGCTC GGAAGATCCC 
AAGTCACTTC CTTCCTCAAA AGAGGTAAAC ATTGTTACCG AAGACACAGC GAAACACAAT 
TTGTCAGAAG AAATAGTGAT TCCAGTGTAT GAAAAGGATC TAAAAGATTT TCAGGAAAAT 
GAAGATGTTG TGAAAACTGA TGAAAGATCG GCAGACATTC TTGAAGAACC AAGTCAGAAT 
ACAGAAGATG AAATGTACAG TCACAGGTCG AAAAATCCCA AGTCGCTTCC TTCCTCAATA 
GAGGTACACA TTGAAACTGA AGATGTTGAG AAGCTGATTT TGTCAGAAGG CTTAAAGAAT 
GTTCTGGATG AAAAATGTCT AGACGATTTT CAGGAAAAGG AAGACGCTGT GAAAACTGAC 
AAAATATTGG CAGATATTCT TGAAGAGCCA AATCAGGATC TAGAAAATAA CACTTGTGAT 
CAGCTGCTTG AAGAACCCAC GCTGCCTCCT TCCTCTAAGG AGGTAATCAC TGCAACAGAA 
GATGCAGAGA AGCTTTCAGA TAGCTTGGAG CATCCTCTGG AAGAAGGAAT TAAAGAAGTA 
GATCTTGTCT CTTTGATTGG AATTAAGCGT GCCCAGGTGT TCGAAGTGGA AAGCACCAAA 
ACAGGGGATG AGGAATATGA TGCTTTGGTA GCTTCCTATT TAGATACCAC TTTACAGGAC 
AGAGAAACCC AAAGTTTACT AATCAATCCA ACCAGTCAAG AAGAAGGACT CTTGGATCCA 
AAAACCCTGG CCTCAATCCT GCCTGAGGAC CCAAACATTA AAAACCAAAC TGTTTCTATG 
TGTACTTCAG AGCATCCAAC AAGCCAACCA GAAAAGCCAG CACTGGTCAG TGGTTCTGAA 
ACATCACCAA GAGCCTTCGA TCATCAGCCT TCAACTGTTC AAACCTCAGC AGAAGAAGAT 
TTAACGTCTG AGCCTGATGA ACTTTCCCAA GAGGCATGTA AAGACCTTGA ACCTCAGCAC 
ACACTGCCAA GAGTCGACAG TGAACTATCT CAAATATCAA AGGATCAGGC TGAGGTCTCT 
CTGAGCACGT GTCTCATGGT CTCCGTAACC TTCTTCACTG CTGTTATCTG CCTGGCTGTA 
GGGATTCAAG AGCCCAGCGC TTTTCTGTGT GTGGGATTAT TCCTGTTGTC CCTTTGGTTC 
TTTGGTTCTC CCATCCACTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-167B20.8 ( XM_003199091.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_003199139.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.

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CloneID	ODa14322
Clone ID Related Accession (Same CDS sequence)	XM_003199091.4 , XM_003199091.5
Accession Version	XM_003199091.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-167b20.8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003199091.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGA CTGCCAAAAA TCTGTCCAGT TCCCCAGAGT CCACCAAAAC TTCCTCCATG 
GCAGGATGTT CTCTTTCCAC TTCAGATATT CCCTACGGAG AAACACTTCC ACTAGAGTCA 
ATGTCATCCA TAAATTCTCC CCAAATAGAA GAGTCTGGCC TTCATCCATC AATTTTATCC 
ATCCCCCAAT CACAGTCAAC CCAGGAGTCC TCAGAGGCAC AAGATCCAGT CTATAATGTT 
CTCCCTTCTG TGTCTTTTTT GGTTCCTGCA GTCCTTGTCC ACTCCTATGA TAAACAAGAG 
GCTAGCAATA ATGAATCTGA GGTGTCCAGC AACCCCAACA CACTGTCTCA TCCACAGAAT 
GGGATGTTAG CGCAAGTGCC TTTCGAGTTG GGCCAACCTT TACTGGCTGC GTTTCCAGAC 
ACGACACATA CCGAGAAAGA AGATCTGGAG GAATTTCAGG AGAAGAAGGA TGTTGTGGAA 
ATGTATGACA AACTGTCAGA CATTCAAGAA GAACCAAGTC AGAATGTAGA AGGAGATAAA 
ACTTGCATTC AGACGTCGGA AAATCCCACA CAGGCCTCTT TCTCAAAAAA GGTAAAAGTT 
GCAACTGAAG ATGTAGAGAA GCTGATTTGG TCAGAGGGCT TGGAGAATCC ACTGAATGAA 
GGAGGTCTAA AAGATTTTCA GGAAAAGGAA GATGTGAAAA TAGACAACAG ATTGGCAAAC 
AGTCAAGAAG AATCAAGTCA GAATATAGAA GAAGATAAAA CTTACATTCA CAGGTCAGAA 
TTTCTTAAAC AGCTTCCTTC CTCAACACAG TTAAACCTTG CAAATGTAGA TTTAGAAAAC 
GTCAATTTGT CAGAGTTTTT AGAGAACCGT CTAGATGAAA ATCCCAAGCA GTCTTCTTCC 
TCAATACAGG TAAATGTTGC AACTGAAGAT GTAGAGAAGC TTAGTTTGTC AGATGGGTTA 
GACAGTTCTC TGGAGAACAG TCTGAAGCAT CTAAAGGAAA ATGAAGATGT TGTGGAAATT 
GTTAACAGCT TGGCAGATAT TCAAGAAGAA CCAAGCCAAA GTATAGAAGA AGATAAAACT 
TACAATTACA AGTCAGAATC TCCCAAACAG CCTTGCTCCT CAATACAGGT AAATGTTGCA 
ACTGAAGATG AAAAGCTCAC TTTGTCGGAT GGGTTAGAGA ATCCTCTGAA AGAAGGTCTG 
AAGGATTTTC AGGAAAATGA AGATGTTGTG AAAACGGTTG ACAGTTTGGC AGATGTTCTA 
GAAGAACCAA GTCAGAATAT AGAAGAAGAT AAAATGTACA GTCACGGCTC GGAAGATCCC 
AAGTCACTTC CTTCCTCAAA AGAGGTAAAC ATTGTTACCG AAGACACAGC GAAACACAAT 
TTGTCAGAAG AAATAGTGAT TCCAGTGTAT GAAAAGGATC TAAAAGATTT TCAGGAAAAT 
GAAGATGTTG TGAAAACTGA TGAAAGATCG GCAGACATTC TTGAAGAACC AAGTCAGAAT 
ACAGAAGATG AAATGTACAG TCACAGGTCG AAAAATCCCA AGTCGCTTCC TTCCTCAATA 
GAGGTACACA TTGAAACTGA AGATGTTGAG AAGCTGATTT TGTCAGAAGG CTTAAAGAAT 
GTTCTGGATG AAAAATGTCT AGACGATTTT CAGGAAAAGG AAGACGCTGT GAAAACTGAC 
AAAATATTGG CAGATATTCT TGAAGAGCCA AATCAGGATC TAGAAAATAA CACTTGTGAT 
CAGCTGCTTG AAGAACCCAC GCTGCCTCCT TCCTCTAAGG AGGTAATCAC TGCAACAGAA 
GATGCAGAGA AGCTTTCAGA TAGCTTGGAG CATCCTCTGG AAGAAGGAAT TAAAGAAGTA 
GATCTTGTCT CTTTGATTGG AATTAAGCGT GCCCAGGTGT TCGAAGTGGA AAGCACCAAA 
ACAGGGGATG AGGAATATGA TGCTTTGGTA GCTTCCTATT TAGATACCAC TTTACAGGAC 
AGAGAAACCC AAAGTTTACT AATCAATCCA ACCAGTCAAG AAGAAGGACT CTTGGATCCA 
AAAACCCTGG CCTCAATCCT GCCTGAGGAC CCAAACATTA AAAACCAAAC TGTTTCTATG 
TGTACTTCAG AGCATCCAAC AAGCCAACCA GAAAAGCCAG CACTGGTCAG TGGTTCTGAA 
ACATCACCAA GAGCCTTCGA TCATCAGCCT TCAACTGTTC AAACCTCAGC AGAAGAAGAT 
TTAACGTCTG AGCCTGATGA ACTTTCCCAA GAGGCATGTA AAGACCTTGA ACCTCAGCAC 
ACACTGCCAA GAGTCGACAG TGAACTATCT CAAATATCAA AGGATCAGGC TGAGGTCTCT 
CTGAGCACGT GTCTCATGGT CTCCGTAACC TTCTTCACTG CTGTTATCTG CCTGGCTGTA 
GGGATTCAAG AGCCCAGCGC TTTTCTGTGT GTGGGATTAT TCCTGTTGTC CCTTTGGTTC 
TTTGGTTCTC CCATCCACTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199139.1
CDS	253..3699
Translation	MAQGNNNNFLSIPSQQGLFKTVRVERSEDNSNDEDEEETEEDVRLIPRSSPVPRKRGSSIADETAEYMRIRLALTDRRVSFVDSMGAELADVRMFVPFDSDEEDNSRWEEEEAKYRKAYSEPTYRLWPEFQVLTGPELLLAVHTNKLEVDSVTPVPDEPLSFDVVIRVLNISFHKSVYVRSTMDGWINHFDYPAEYVQDSNDGETDKFSVKLSFAPPYLFNGARIDFVVRYETSDGEFWANNSGRNYSVTLLQSYDEDSVQTCAAEKPELRGILKPPRYRTASGYDDSDDREDGNLSNDECEAPENQDYFAQPTIVQPEIDIETAKNLSSSPESTKTSSMAGCSLSTSDIPYGETLPLESMSSINSPQIEESGLHPSILSIPQSQSTQESSEAQDPVYNVLPSVSFLVPAVLVHSYDKQEASNNESEVSSNPNTLSHPQNGMLAQVPFELGQPLLAAFPDTTHTEKEDLEEFQEKKDVVEMYDKLSDIQEEPSQNVEGDKTCIQTSENPTQASFSKKVKVATEDVEKLIWSEGLENPLNEGGLKDFQEKEDVKIDNRLANSQEESSQNIEEDKTYIHRSEFLKQLPSSTQLNLANVDLENVNLSEFLENRLDENPKQSSSSIQVNVATEDVEKLSLSDGLDSSLENSLKHLKENEDVVEIVNSLADIQEEPSQSIEEDKTYNYKSESPKQPCSSIQVNVATEDEKLTLSDGLENPLKEGLKDFQENEDVVKTVDSLADVLEEPSQNIEEDKMYSHGSEDPKSLPSSKEVNIVTEDTAKHNLSEEIVIPVYEKDLKDFQENEDVVKTDERSADILEEPSQNTEDEMYSHRSKNPKSLPSSIEVHIETEDVEKLILSEGLKNVLDEKCLDDFQEKEDAVKTDKILADILEEPNQDLENNTCDQLLEEPTLPPSSKEVITATEDAEKLSDSLEHPLEEGIKEVDLVSLIGIKRAQVFEVESTKTGDEEYDALVASYLDTTLQDRETQSLLINPTSQEEGLLDPKTLASILPEDPNIKNQTVSMCTSEHPTSQPEKPALVSGSETSPRAFDHQPSTVQTSAEEDLTSEPDELSQEACKDLEPQHTLPRVDSELSQISKDQAEVSLSTCLMVSVTFFTAVICLAVGIQEPSAFLCVGLFLLSLWFFGSPIHFS

Target ORF information:

RefSeq Version	XM_003199091.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199091.5

ORF Insert Sequence:

ATGGCGCAGG GAAATAACAA CAATTTCCTA TCTATCCCCT CACAACAGGG TCTTTTCAAG 
ACGGTTAGAG TTGAACGTTC TGAAGACAAT AGTAACGATG AAGATGAAGA AGAAACAGAG 
GAAGACGTCC GGCTTATACC GAGGTCTTCC CCCGTTCCCA GGAAGCGAGG TTCGTCCATC 
GCGGATGAAA CCGCCGAGTA CATGCGAATT CGTCTCGCGT TAACTGATAG AAGAGTCTCG 
TTTGTAGACA GTATGGGAGC CGAGCTCGCT GATGTCAGAA TGTTTGTCCC GTTTGATTCA 
GACGAAGAGG ACAATTCAAG ATGGGAAGAA GAGGAGGCGA AATACCGTAA AGCCTACAGC 
GAGCCCACTT ACCGTCTGTG GCCGGAGTTC CAGGTGCTCA CCGGCCCGGA GCTTTTACTC 
GCGGTCCACA CCAACAAGCT GGAGGTGGAC AGCGTGACAC CTGTGCCAGA CGAGCCTCTA 
TCCTTCGACG TGGTCATTCG TGTGCTTAAC ATTTCTTTTC ACAAATCAGT CTATGTCAGG 
TCGACTATGG ACGGTTGGAT CAATCACTTT GATTACCCAG CCGAGTACGT CCAGGACTCA 
AACGACGGCG AAACGGACAA GTTCTCCGTG AAGCTGTCCT TTGCTCCGCC GTACTTGTTC 
AACGGAGCAC GCATTGATTT TGTTGTTCGA TATGAGACGT CAGATGGAGA GTTTTGGGCG 
AACAACTCTG GAAGGAATTA TTCGGTAACT CTCCTACAGT CTTATGATGA AGACTCGGTC 
CAGACCTGCG CGGCGGAGAA GCCAGAGCTG AGAGGAATCC TGAAACCTCC CCGGTATCGA 
ACGGCCAGTG GTTATGATGA CTCTGATGAC AGAGAAGATG GAAATCTAAG CAATGACGAG 
TGTGAAGCAC CGGAAAATCA AGACTATTTT GCACAGCCAA CCATAGTTCA GCCAGAGATC 
GACATCGAGA CTGCCAAAAA TCTGTCCAGT TCCCCAGAGT CCACCAAAAC TTCCTCCATG 
GCAGGATGTT CTCTTTCCAC TTCAGATATT CCCTACGGAG AAACACTTCC ACTAGAGTCA 
ATGTCATCCA TAAATTCTCC CCAAATAGAA GAGTCTGGCC TTCATCCATC AATTTTATCC 
ATCCCCCAAT CACAGTCAAC CCAGGAGTCC TCAGAGGCAC AAGATCCAGT CTATAATGTT 
CTCCCTTCTG TGTCTTTTTT GGTTCCTGCA GTCCTTGTCC ACTCCTATGA TAAACAAGAG 
GCTAGCAATA ATGAATCTGA GGTGTCCAGC AACCCCAACA CACTGTCTCA TCCACAGAAT 
GGGATGTTAG CGCAAGTGCC TTTCGAGTTG GGCCAACCTT TACTGGCTGC GTTTCCAGAC 
ACGACACATA CCGAGAAAGA AGATCTGGAG GAATTTCAGG AGAAGAAGGA TGTTGTGGAA 
ATGTATGACA AACTGTCAGA CATTCAAGAA GAACCAAGTC AGAATGTAGA AGGAGATAAA 
ACTTGCATTC AGACGTCGGA AAATCCCACA CAGGCCTCTT TCTCAAAAAA GGTAAAAGTT 
GCAACTGAAG ATGTAGAGAA GCTGATTTGG TCAGAGGGCT TGGAGAATCC ACTGAATGAA 
GGAGGTCTAA AAGATTTTCA GGAAAAGGAA GATGTGAAAA TAGACAACAG ATTGGCAAAC 
AGTCAAGAAG AATCAAGTCA GAATATAGAA GAAGATAAAA CTTACATTCA CAGGTCAGAA 
TTTCTTAAAC AGCTTCCTTC CTCAACACAG TTAAACCTTG CAAATGTAGA TTTAGAAAAC 
GTCAATTTGT CAGAGTTTTT AGAGAACCGT CTAGATGAAA ATCCCAAGCA GTCTTCTTCC 
TCAATACAGG TAAATGTTGC AACTGAAGAT GTAGAGAAGC TTAGTTTGTC AGATGGGTTA 
GACAGTTCTC TGGAGAACAG TCTGAAGCAT CTAAAGGAAA ATGAAGATGT TGTGGAAATT 
GTTAACAGCT TGGCAGATAT TCAAGAAGAA CCAAGCCAAA GTATAGAAGA AGATAAAACT 
TACAATTACA AGTCAGAATC TCCCAAACAG CCTTGCTCCT CAATACAGGT AAATGTTGCA 
ACTGAAGATG AAAAGCTCAC TTTGTCGGAT GGGTTAGAGA ATCCTCTGAA AGAAGGTCTG 
AAGGATTTTC AGGAAAATGA AGATGTTGTG AAAACGGTTG ACAGTTTGGC AGATGTTCTA 
GAAGAACCAA GTCAGAATAT AGAAGAAGAT AAAATGTACA GTCACGGCTC GGAAGATCCC 
AAGTCACTTC CTTCCTCAAA AGAGGTAAAC ATTGTTACCG AAGACACAGC GAAACACAAT 
TTGTCAGAAG AAATAGTGAT TCCAGTGTAT GAAAAGGATC TAAAAGATTT TCAGGAAAAT 
GAAGATGTTG TGAAAACTGA TGAAAGATCG GCAGACATTC TTGAAGAACC AAGTCAGAAT 
ACAGAAGATG AAATGTACAG TCACAGGTCG AAAAATCCCA AGTCGCTTCC TTCCTCAATA 
GAGGTACACA TTGAAACTGA AGATGTTGAG AAGCTGATTT TGTCAGAAGG CTTAAAGAAT 
GTTCTGGATG AAAAATGTCT AGACGATTTT CAGGAAAAGG AAGACGCTGT GAAAACTGAC 
AAAATATTGG CAGATATTCT TGAAGAGCCA AATCAGGATC TAGAAAATAA CACTTGTGAT 
CAGCTGCTTG AAGAACCCAC GCTGCCTCCT TCCTCTAAGG AGGTAATCAC TGCAACAGAA 
GATGCAGAGA AGCTTTCAGA TAGCTTGGAG CATCCTCTGG AAGAAGGAAT TAAAGAAGTA 
GATCTTGTCT CTTTGATTGG AATTAAGCGT GCCCAGGTGT TCGAAGTGGA AAGCACCAAA 
ACAGGGGATG AGGAATATGA TGCTTTGGTA GCTTCCTATT TAGATACCAC TTTACAGGAC 
AGAGAAACCC AAAGTTTACT AATCAATCCA ACCAGTCAAG AAGAAGGACT CTTGGATCCA 
AAAACCCTGG CCTCAATCCT GCCTGAGGAC CCAAACATTA AAAACCAAAC TGTTTCTATG 
TGTACTTCAG AGCATCCAAC AAGCCAACCA GAAAAGCCAG CACTGGTCAG TGGTTCTGAA 
ACATCACCAA GAGCCTTCGA TCATCAGCCT TCAACTGTTC AAACCTCAGC AGAAGAAGAT 
TTAACGTCTG AGCCTGATGA ACTTTCCCAA GAGGCATGTA AAGACCTTGA ACCTCAGCAC 
ACACTGCCAA GAGTCGACAG TGAACTATCT CAAATATCAA AGGATCAGGC TGAGGTCTCT 
CTGAGCACGT GTCTCATGGT CTCCGTAACC TTCTTCACTG CTGTTATCTG CCTGGCTGTA 
GGGATTCAAG AGCCCAGCGC TTTTCTGTGT GTGGGATTAT TCCTGTTGTC CCTTTGGTTC 
TTTGGTTCTC CCATCCACTT CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-167B20.8 ( XM_005167121.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005167178.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.

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CloneID	ODa14323
Clone ID Related Accession (Same CDS sequence)	XM_005167121.3 , XM_021478446.1
Accession Version	XM_005167121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein si:ch211-167b20.8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAT GTTCTCTTTC CACTTCAGAT ATTCCCTACG GAGAAACACT TCCACTAGAG 
TCAATGTCAT CCATAAATTC TCCCCAAATA GAAGAGTCTG GCCTTCATCC ATCAATTTTA 
TCCATCCCCC AATCACAGTC AACCCAGGAG TCCTCAGAGG CACAAGATCC AGTCTATAAT 
GTTCTCCCTT CTGTGTCTTT TTTGGTTCCT GCAGTCCTTG TCCACTCCTA TGATAAACAA 
GAGGCTAGCA ATAATGAATC TGAGGTGTCC AGCAACCCCA ACACACTGTC TCATCCACAG 
AATGGGATGT TAGCGCAAGT GCCTTTCGAG TTGGGCCAAC CTTTACTGGC TGCGTTTCCA 
GACACGACAC ATACCGAGAA AGAAGATCTG GAGGAATTTC AGGAGAAGAA GGATGTTGTG 
GAAATGTATG ACAAACTGTC AGACATTCAA GAAGAACCAA GTCAGAATGT AGAAGGAGAT 
AAAACTTGCA TTCAGACGTC GGAAAATCCC ACACAGGCCT CTTTCTCAAA AAAGGTAAAA 
GTTGCAACTG AAGATGTAGA GAAGCTGATT TGGTCAGAGG GCTTGGAGAA TCCACTGAAT 
GAAGGAGGTC TAAAAGATTT TCAGGAAAAG GAAGATGTGA AAATAGACAA CAGATTGGCA 
AACAGTCAAG AAGAATCAAG TCAGAATATA GAAGAAGATA AAACTTACAT TCACAGGTCA 
GAATTTCTTA AACAGCTTCC TTCCTCAACA CAGTTAAACC TTGCAAATGT AGATTTAGAA 
AACGTCAATT TGTCAGAGTT TTTAGAGAAC CGTCTAGATG AAAATCCCAA GCAGTCTTCT 
TCCTCAATAC AGGTAAATGT TGCAACTGAA GATGTAGAGA AGCTTAGTTT GTCAGATGGG 
TTAGACAGTT CTCTGGAGAA CAGTCTGAAG CATCTAAAGG AAAATGAAGA TGTTGTGGAA 
ATTGTTAACA GCTTGGCAGA TATTCAAGAA GAACCAAGCC AAAGTATAGA AGAAGATAAA 
ACTTACAATT ACAAGTCAGA ATCTCCCAAA CAGCCTTGCT CCTCAATACA GGTAAATGTT 
GCAACTGAAG ATGAAAAGCT CACTTTGTCG GATGGGTTAG AGAATCCTCT GAAAGAAGGT 
CTGAAGGATT TTCAGGAAAA TGAAGATGTT GTGAAAACGG TTGACAGTTT GGCAGATGTT 
CTAGAAGAAC CAAGTCAGAA TATAGAAGAA GATAAAATGT ACAGTCACGG CTCGGAAGAT 
CCCAAGTCAC TTCCTTCCTC AAAAGAGGTA AACATTGTTA CCGAAGACAC AGCGAAACAC 
AATTTGTCAG AAGAAATAGT GATTCCAGTG TATGAAAAGG ATCTAAAAGA TTTTCAGGAA 
AATGAAGATG TTGTGAAAAC TGATGAAAGA TCGGCAGACA TTCTTGAAGA ACCAAGTCAG 
AATACAGAAG ATGAAATGTA CAGTCACAGG TCGAAAAATC CCAAGTCGCT TCCTTCCTCA 
ATAGAGGTAC ACATTGAAAC TGAAGATGTT GAGAAGCTGA TTTTGTCAGA AGGCTTAAAG 
AATGTTCTGG ATGAAAAATG TCTAGACGAT TTTCAGGAAA AGGAAGACGC TGTGAAAACT 
GACAAAATAT TGGCAGATAT TCTTGAAGAG CCAAATCAGG ATCTAGAAAA TAACACTTGT 
GATCAGCTGC TTGAAGAACC CACGCTGCCT CCTTCCTCTA AGGAGGTAAT CACTGCAACA 
GAAGATGCAG AGAAGCTTTC AGATAGCTTG GAGCATCCTC TGGAAGAAGG AATTAAAGAA 
GTAGATCTTG TCTCTTTGAT TGGAATTAAG CGTGCCCAGG TGTTCGAAGT GGAAAGCACC 
AAAACAGGGG ATGAGGAATA TGATGCTTTG GTAGCTTCCT ATTTAGATAC CACTTTACAG 
GACAGAGAAA CCCAAAGTTT ACTAATCAAT CCAACCAGTC AAGAAGAAGG ACTCTTGGAT 
CCAAAAACCC TGGCCTCAAT CCTGCCTGAG GACCCAAACA TTAAAAACCA AACTGTTTCT 
ATGTGTACTT CAGAGCATCC AACAAGCCAA CCAGAAAAGC CAGCACTGGT CAGTGGTTCT 
GAAACATCAC CAAGAGCCTT CGATCATCAG CCTTCAACTG TTCAAACCTC AGCAGAAGAA 
GATTTAACGT CTGAGCCTGA TGAACTTTCC CAAGAGGCAT GTAAAGACCT TGAACCTCAG 
CACACACTGC CAAGAGTCGA CAGTGAACTA TCTCAAATAT CAAAGGATCA GGCTGAGGTC 
TCTCTGAGCA CGTGTCTCAT GGTCTCCGTA ACCTTCTTCA CTGCTGTTAT CTGCCTGGCT 
GTAGGGATTC AAGAGCCCAG CGCTTTTCTG TGTGTGGGAT TATTCCTGTT GTCCCTTTGG 
TTCTTTGGTT CTCCCATCCA CTTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167178.1
CDS	78..2507
Translation	MAGCSLSTSDIPYGETLPLESMSSINSPQIEESGLHPSILSIPQSQSTQESSEAQDPVYNVLPSVSFLVPAVLVHSYDKQEASNNESEVSSNPNTLSHPQNGMLAQVPFELGQPLLAAFPDTTHTEKEDLEEFQEKKDVVEMYDKLSDIQEEPSQNVEGDKTCIQTSENPTQASFSKKVKVATEDVEKLIWSEGLENPLNEGGLKDFQEKEDVKIDNRLANSQEESSQNIEEDKTYIHRSEFLKQLPSSTQLNLANVDLENVNLSEFLENRLDENPKQSSSSIQVNVATEDVEKLSLSDGLDSSLENSLKHLKENEDVVEIVNSLADIQEEPSQSIEEDKTYNYKSESPKQPCSSIQVNVATEDEKLTLSDGLENPLKEGLKDFQENEDVVKTVDSLADVLEEPSQNIEEDKMYSHGSEDPKSLPSSKEVNIVTEDTAKHNLSEEIVIPVYEKDLKDFQENEDVVKTDERSADILEEPSQNTEDEMYSHRSKNPKSLPSSIEVHIETEDVEKLILSEGLKNVLDEKCLDDFQEKEDAVKTDKILADILEEPNQDLENNTCDQLLEEPTLPPSSKEVITATEDAEKLSDSLEHPLEEGIKEVDLVSLIGIKRAQVFEVESTKTGDEEYDALVASYLDTTLQDRETQSLLINPTSQEEGLLDPKTLASILPEDPNIKNQTVSMCTSEHPTSQPEKPALVSGSETSPRAFDHQPSTVQTSAEEDLTSEPDELSQEACKDLEPQHTLPRVDSELSQISKDQAEVSLSTCLMVSVTFFTAVICLAVGIQEPSAFLCVGLFLLSLWFFGSPIHFS

Target ORF information:

RefSeq Version	XM_005167121.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167121.3

ORF Insert Sequence:

ATGGCAGGAT GTTCTCTTTC CACTTCAGAT ATTCCCTACG GAGAAACACT TCCACTAGAG 
TCAATGTCAT CCATAAATTC TCCCCAAATA GAAGAGTCTG GCCTTCATCC ATCAATTTTA 
TCCATCCCCC AATCACAGTC AACCCAGGAG TCCTCAGAGG CACAAGATCC AGTCTATAAT 
GTTCTCCCTT CTGTGTCTTT TTTGGTTCCT GCAGTCCTTG TCCACTCCTA TGATAAACAA 
GAGGCTAGCA ATAATGAATC TGAGGTGTCC AGCAACCCCA ACACACTGTC TCATCCACAG 
AATGGGATGT TAGCGCAAGT GCCTTTCGAG TTGGGCCAAC CTTTACTGGC TGCGTTTCCA 
GACACGACAC ATACCGAGAA AGAAGATCTG GAGGAATTTC AGGAGAAGAA GGATGTTGTG 
GAAATGTATG ACAAACTGTC AGACATTCAA GAAGAACCAA GTCAGAATGT AGAAGGAGAT 
AAAACTTGCA TTCAGACGTC GGAAAATCCC ACACAGGCCT CTTTCTCAAA AAAGGTAAAA 
GTTGCAACTG AAGATGTAGA GAAGCTGATT TGGTCAGAGG GCTTGGAGAA TCCACTGAAT 
GAAGGAGGTC TAAAAGATTT TCAGGAAAAG GAAGATGTGA AAATAGACAA CAGATTGGCA 
AACAGTCAAG AAGAATCAAG TCAGAATATA GAAGAAGATA AAACTTACAT TCACAGGTCA 
GAATTTCTTA AACAGCTTCC TTCCTCAACA CAGTTAAACC TTGCAAATGT AGATTTAGAA 
AACGTCAATT TGTCAGAGTT TTTAGAGAAC CGTCTAGATG AAAATCCCAA GCAGTCTTCT 
TCCTCAATAC AGGTAAATGT TGCAACTGAA GATGTAGAGA AGCTTAGTTT GTCAGATGGG 
TTAGACAGTT CTCTGGAGAA CAGTCTGAAG CATCTAAAGG AAAATGAAGA TGTTGTGGAA 
ATTGTTAACA GCTTGGCAGA TATTCAAGAA GAACCAAGCC AAAGTATAGA AGAAGATAAA 
ACTTACAATT ACAAGTCAGA ATCTCCCAAA CAGCCTTGCT CCTCAATACA GGTAAATGTT 
GCAACTGAAG ATGAAAAGCT CACTTTGTCG GATGGGTTAG AGAATCCTCT GAAAGAAGGT 
CTGAAGGATT TTCAGGAAAA TGAAGATGTT GTGAAAACGG TTGACAGTTT GGCAGATGTT 
CTAGAAGAAC CAAGTCAGAA TATAGAAGAA GATAAAATGT ACAGTCACGG CTCGGAAGAT 
CCCAAGTCAC TTCCTTCCTC AAAAGAGGTA AACATTGTTA CCGAAGACAC AGCGAAACAC 
AATTTGTCAG AAGAAATAGT GATTCCAGTG TATGAAAAGG ATCTAAAAGA TTTTCAGGAA 
AATGAAGATG TTGTGAAAAC TGATGAAAGA TCGGCAGACA TTCTTGAAGA ACCAAGTCAG 
AATACAGAAG ATGAAATGTA CAGTCACAGG TCGAAAAATC CCAAGTCGCT TCCTTCCTCA 
ATAGAGGTAC ACATTGAAAC TGAAGATGTT GAGAAGCTGA TTTTGTCAGA AGGCTTAAAG 
AATGTTCTGG ATGAAAAATG TCTAGACGAT TTTCAGGAAA AGGAAGACGC TGTGAAAACT 
GACAAAATAT TGGCAGATAT TCTTGAAGAG CCAAATCAGG ATCTAGAAAA TAACACTTGT 
GATCAGCTGC TTGAAGAACC CACGCTGCCT CCTTCCTCTA AGGAGGTAAT CACTGCAACA 
GAAGATGCAG AGAAGCTTTC AGATAGCTTG GAGCATCCTC TGGAAGAAGG AATTAAAGAA 
GTAGATCTTG TCTCTTTGAT TGGAATTAAG CGTGCCCAGG TGTTCGAAGT GGAAAGCACC 
AAAACAGGGG ATGAGGAATA TGATGCTTTG GTAGCTTCCT ATTTAGATAC CACTTTACAG 
GACAGAGAAA CCCAAAGTTT ACTAATCAAT CCAACCAGTC AAGAAGAAGG ACTCTTGGAT 
CCAAAAACCC TGGCCTCAAT CCTGCCTGAG GACCCAAACA TTAAAAACCA AACTGTTTCT 
ATGTGTACTT CAGAGCATCC AACAAGCCAA CCAGAAAAGC CAGCACTGGT CAGTGGTTCT 
GAAACATCAC CAAGAGCCTT CGATCATCAG CCTTCAACTG TTCAAACCTC AGCAGAAGAA 
GATTTAACGT CTGAGCCTGA TGAACTTTCC CAAGAGGCAT GTAAAGACCT TGAACCTCAG 
CACACACTGC CAAGAGTCGA CAGTGAACTA TCTCAAATAT CAAAGGATCA GGCTGAGGTC 
TCTCTGAGCA CGTGTCTCAT GGTCTCCGTA ACCTTCTTCA CTGCTGTTAT CTGCCTGGCT 
GTAGGGATTC AAGAGCCCAG CGCTTTTCTG TGTGTGGGAT TATTCCTGTT GTCCCTTTGG 
TTCTTTGGTT CTCCCATCCA CTTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-167B20.8 ( XM_021478446.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021334121.1 Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.

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CloneID	ODa14323
Clone ID Related Accession (Same CDS sequence)	XM_005167121.3 , XM_021478446.1
Accession Version	XM_021478446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein si:ch211-167b20.8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGAT GTTCTCTTTC CACTTCAGAT ATTCCCTACG GAGAAACACT TCCACTAGAG 
TCAATGTCAT CCATAAATTC TCCCCAAATA GAAGAGTCTG GCCTTCATCC ATCAATTTTA 
TCCATCCCCC AATCACAGTC AACCCAGGAG TCCTCAGAGG CACAAGATCC AGTCTATAAT 
GTTCTCCCTT CTGTGTCTTT TTTGGTTCCT GCAGTCCTTG TCCACTCCTA TGATAAACAA 
GAGGCTAGCA ATAATGAATC TGAGGTGTCC AGCAACCCCA ACACACTGTC TCATCCACAG 
AATGGGATGT TAGCGCAAGT GCCTTTCGAG TTGGGCCAAC CTTTACTGGC TGCGTTTCCA 
GACACGACAC ATACCGAGAA AGAAGATCTG GAGGAATTTC AGGAGAAGAA GGATGTTGTG 
GAAATGTATG ACAAACTGTC AGACATTCAA GAAGAACCAA GTCAGAATGT AGAAGGAGAT 
AAAACTTGCA TTCAGACGTC GGAAAATCCC ACACAGGCCT CTTTCTCAAA AAAGGTAAAA 
GTTGCAACTG AAGATGTAGA GAAGCTGATT TGGTCAGAGG GCTTGGAGAA TCCACTGAAT 
GAAGGAGGTC TAAAAGATTT TCAGGAAAAG GAAGATGTGA AAATAGACAA CAGATTGGCA 
AACAGTCAAG AAGAATCAAG TCAGAATATA GAAGAAGATA AAACTTACAT TCACAGGTCA 
GAATTTCTTA AACAGCTTCC TTCCTCAACA CAGTTAAACC TTGCAAATGT AGATTTAGAA 
AACGTCAATT TGTCAGAGTT TTTAGAGAAC CGTCTAGATG AAAATCCCAA GCAGTCTTCT 
TCCTCAATAC AGGTAAATGT TGCAACTGAA GATGTAGAGA AGCTTAGTTT GTCAGATGGG 
TTAGACAGTT CTCTGGAGAA CAGTCTGAAG CATCTAAAGG AAAATGAAGA TGTTGTGGAA 
ATTGTTAACA GCTTGGCAGA TATTCAAGAA GAACCAAGCC AAAGTATAGA AGAAGATAAA 
ACTTACAATT ACAAGTCAGA ATCTCCCAAA CAGCCTTGCT CCTCAATACA GGTAAATGTT 
GCAACTGAAG ATGAAAAGCT CACTTTGTCG GATGGGTTAG AGAATCCTCT GAAAGAAGGT 
CTGAAGGATT TTCAGGAAAA TGAAGATGTT GTGAAAACGG TTGACAGTTT GGCAGATGTT 
CTAGAAGAAC CAAGTCAGAA TATAGAAGAA GATAAAATGT ACAGTCACGG CTCGGAAGAT 
CCCAAGTCAC TTCCTTCCTC AAAAGAGGTA AACATTGTTA CCGAAGACAC AGCGAAACAC 
AATTTGTCAG AAGAAATAGT GATTCCAGTG TATGAAAAGG ATCTAAAAGA TTTTCAGGAA 
AATGAAGATG TTGTGAAAAC TGATGAAAGA TCGGCAGACA TTCTTGAAGA ACCAAGTCAG 
AATACAGAAG ATGAAATGTA CAGTCACAGG TCGAAAAATC CCAAGTCGCT TCCTTCCTCA 
ATAGAGGTAC ACATTGAAAC TGAAGATGTT GAGAAGCTGA TTTTGTCAGA AGGCTTAAAG 
AATGTTCTGG ATGAAAAATG TCTAGACGAT TTTCAGGAAA AGGAAGACGC TGTGAAAACT 
GACAAAATAT TGGCAGATAT TCTTGAAGAG CCAAATCAGG ATCTAGAAAA TAACACTTGT 
GATCAGCTGC TTGAAGAACC CACGCTGCCT CCTTCCTCTA AGGAGGTAAT CACTGCAACA 
GAAGATGCAG AGAAGCTTTC AGATAGCTTG GAGCATCCTC TGGAAGAAGG AATTAAAGAA 
GTAGATCTTG TCTCTTTGAT TGGAATTAAG CGTGCCCAGG TGTTCGAAGT GGAAAGCACC 
AAAACAGGGG ATGAGGAATA TGATGCTTTG GTAGCTTCCT ATTTAGATAC CACTTTACAG 
GACAGAGAAA CCCAAAGTTT ACTAATCAAT CCAACCAGTC AAGAAGAAGG ACTCTTGGAT 
CCAAAAACCC TGGCCTCAAT CCTGCCTGAG GACCCAAACA TTAAAAACCA AACTGTTTCT 
ATGTGTACTT CAGAGCATCC AACAAGCCAA CCAGAAAAGC CAGCACTGGT CAGTGGTTCT 
GAAACATCAC CAAGAGCCTT CGATCATCAG CCTTCAACTG TTCAAACCTC AGCAGAAGAA 
GATTTAACGT CTGAGCCTGA TGAACTTTCC CAAGAGGCAT GTAAAGACCT TGAACCTCAG 
CACACACTGC CAAGAGTCGA CAGTGAACTA TCTCAAATAT CAAAGGATCA GGCTGAGGTC 
TCTCTGAGCA CGTGTCTCAT GGTCTCCGTA ACCTTCTTCA CTGCTGTTAT CTGCCTGGCT 
GTAGGGATTC AAGAGCCCAG CGCTTTTCTG TGTGTGGGAT TATTCCTGTT GTCCCTTTGG 
TTCTTTGGTT CTCCCATCCA CTTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334121.1
CDS	165..2594
Translation	MAGCSLSTSDIPYGETLPLESMSSINSPQIEESGLHPSILSIPQSQSTQESSEAQDPVYNVLPSVSFLVPAVLVHSYDKQEASNNESEVSSNPNTLSHPQNGMLAQVPFELGQPLLAAFPDTTHTEKEDLEEFQEKKDVVEMYDKLSDIQEEPSQNVEGDKTCIQTSENPTQASFSKKVKVATEDVEKLIWSEGLENPLNEGGLKDFQEKEDVKIDNRLANSQEESSQNIEEDKTYIHRSEFLKQLPSSTQLNLANVDLENVNLSEFLENRLDENPKQSSSSIQVNVATEDVEKLSLSDGLDSSLENSLKHLKENEDVVEIVNSLADIQEEPSQSIEEDKTYNYKSESPKQPCSSIQVNVATEDEKLTLSDGLENPLKEGLKDFQENEDVVKTVDSLADVLEEPSQNIEEDKMYSHGSEDPKSLPSSKEVNIVTEDTAKHNLSEEIVIPVYEKDLKDFQENEDVVKTDERSADILEEPSQNTEDEMYSHRSKNPKSLPSSIEVHIETEDVEKLILSEGLKNVLDEKCLDDFQEKEDAVKTDKILADILEEPNQDLENNTCDQLLEEPTLPPSSKEVITATEDAEKLSDSLEHPLEEGIKEVDLVSLIGIKRAQVFEVESTKTGDEEYDALVASYLDTTLQDRETQSLLINPTSQEEGLLDPKTLASILPEDPNIKNQTVSMCTSEHPTSQPEKPALVSGSETSPRAFDHQPSTVQTSAEEDLTSEPDELSQEACKDLEPQHTLPRVDSELSQISKDQAEVSLSTCLMVSVTFFTAVICLAVGIQEPSAFLCVGLFLLSLWFFGSPIHFS

Target ORF information:

RefSeq Version	XM_021478446.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-167b20.8 (si:ch211-167b20.8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478446.1

ORF Insert Sequence:

ATGGCAGGAT GTTCTCTTTC CACTTCAGAT ATTCCCTACG GAGAAACACT TCCACTAGAG 
TCAATGTCAT CCATAAATTC TCCCCAAATA GAAGAGTCTG GCCTTCATCC ATCAATTTTA 
TCCATCCCCC AATCACAGTC AACCCAGGAG TCCTCAGAGG CACAAGATCC AGTCTATAAT 
GTTCTCCCTT CTGTGTCTTT TTTGGTTCCT GCAGTCCTTG TCCACTCCTA TGATAAACAA 
GAGGCTAGCA ATAATGAATC TGAGGTGTCC AGCAACCCCA ACACACTGTC TCATCCACAG 
AATGGGATGT TAGCGCAAGT GCCTTTCGAG TTGGGCCAAC CTTTACTGGC TGCGTTTCCA 
GACACGACAC ATACCGAGAA AGAAGATCTG GAGGAATTTC AGGAGAAGAA GGATGTTGTG 
GAAATGTATG ACAAACTGTC AGACATTCAA GAAGAACCAA GTCAGAATGT AGAAGGAGAT 
AAAACTTGCA TTCAGACGTC GGAAAATCCC ACACAGGCCT CTTTCTCAAA AAAGGTAAAA 
GTTGCAACTG AAGATGTAGA GAAGCTGATT TGGTCAGAGG GCTTGGAGAA TCCACTGAAT 
GAAGGAGGTC TAAAAGATTT TCAGGAAAAG GAAGATGTGA AAATAGACAA CAGATTGGCA 
AACAGTCAAG AAGAATCAAG TCAGAATATA GAAGAAGATA AAACTTACAT TCACAGGTCA 
GAATTTCTTA AACAGCTTCC TTCCTCAACA CAGTTAAACC TTGCAAATGT AGATTTAGAA 
AACGTCAATT TGTCAGAGTT TTTAGAGAAC CGTCTAGATG AAAATCCCAA GCAGTCTTCT 
TCCTCAATAC AGGTAAATGT TGCAACTGAA GATGTAGAGA AGCTTAGTTT GTCAGATGGG 
TTAGACAGTT CTCTGGAGAA CAGTCTGAAG CATCTAAAGG AAAATGAAGA TGTTGTGGAA 
ATTGTTAACA GCTTGGCAGA TATTCAAGAA GAACCAAGCC AAAGTATAGA AGAAGATAAA 
ACTTACAATT ACAAGTCAGA ATCTCCCAAA CAGCCTTGCT CCTCAATACA GGTAAATGTT 
GCAACTGAAG ATGAAAAGCT CACTTTGTCG GATGGGTTAG AGAATCCTCT GAAAGAAGGT 
CTGAAGGATT TTCAGGAAAA TGAAGATGTT GTGAAAACGG TTGACAGTTT GGCAGATGTT 
CTAGAAGAAC CAAGTCAGAA TATAGAAGAA GATAAAATGT ACAGTCACGG CTCGGAAGAT 
CCCAAGTCAC TTCCTTCCTC AAAAGAGGTA AACATTGTTA CCGAAGACAC AGCGAAACAC 
AATTTGTCAG AAGAAATAGT GATTCCAGTG TATGAAAAGG ATCTAAAAGA TTTTCAGGAA 
AATGAAGATG TTGTGAAAAC TGATGAAAGA TCGGCAGACA TTCTTGAAGA ACCAAGTCAG 
AATACAGAAG ATGAAATGTA CAGTCACAGG TCGAAAAATC CCAAGTCGCT TCCTTCCTCA 
ATAGAGGTAC ACATTGAAAC TGAAGATGTT GAGAAGCTGA TTTTGTCAGA AGGCTTAAAG 
AATGTTCTGG ATGAAAAATG TCTAGACGAT TTTCAGGAAA AGGAAGACGC TGTGAAAACT 
GACAAAATAT TGGCAGATAT TCTTGAAGAG CCAAATCAGG ATCTAGAAAA TAACACTTGT 
GATCAGCTGC TTGAAGAACC CACGCTGCCT CCTTCCTCTA AGGAGGTAAT CACTGCAACA 
GAAGATGCAG AGAAGCTTTC AGATAGCTTG GAGCATCCTC TGGAAGAAGG AATTAAAGAA 
GTAGATCTTG TCTCTTTGAT TGGAATTAAG CGTGCCCAGG TGTTCGAAGT GGAAAGCACC 
AAAACAGGGG ATGAGGAATA TGATGCTTTG GTAGCTTCCT ATTTAGATAC CACTTTACAG 
GACAGAGAAA CCCAAAGTTT ACTAATCAAT CCAACCAGTC AAGAAGAAGG ACTCTTGGAT 
CCAAAAACCC TGGCCTCAAT CCTGCCTGAG GACCCAAACA TTAAAAACCA AACTGTTTCT 
ATGTGTACTT CAGAGCATCC AACAAGCCAA CCAGAAAAGC CAGCACTGGT CAGTGGTTCT 
GAAACATCAC CAAGAGCCTT CGATCATCAG CCTTCAACTG TTCAAACCTC AGCAGAAGAA 
GATTTAACGT CTGAGCCTGA TGAACTTTCC CAAGAGGCAT GTAAAGACCT TGAACCTCAG 
CACACACTGC CAAGAGTCGA CAGTGAACTA TCTCAAATAT CAAAGGATCA GGCTGAGGTC 
TCTCTGAGCA CGTGTCTCAT GGTCTCCGTA ACCTTCTTCA CTGCTGTTAT CTGCCTGGCT 
GTAGGGATTC AAGAGCCCAG CGCTTTTCTG TGTGTGGGAT TATTCCTGTT GTCCCTTTGG 
TTCTTTGGTT CTCCCATCCA CTTCTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI:CH211-167B20.8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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