nid2b cDNA ORF clone, Danio rerio(zebrafish)

The following nid2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nid2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146071 XM_021473357.1
Latest version!
Danio rerio nidogen 2b (osteonidogen) (nid2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
ODa56747 XM_017352728.1
Latest version!
Danio rerio nidogen 2b (osteonidogen) (nid2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa150674 XM_021468530.1
Latest version!
Danio rerio nidogen 2b (osteonidogen) (nid2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146071
    Clone ID Related Accession (Same CDS sequence) XM_021473357.1
    Accession Version XM_021473357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395063.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCCCTCT GTTTTTCTTT AAAAAAAAAT TCTGCAGCAA AAAGGCCCAA GCCTTCATTA 
    ACTGCATGCC AGCAGCACAG AAACAGCCTG CAGGAGAGTA TGAGCATGTA TCCAGCTCTG
    GAGGCTTTTG TTCCAAAGTG TGATGATAAA GGCCAGTACA AACCCCTGCA GTGTTTGGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGC AGGGGGCAGG AGAGAGTGGG CACCAGGACA
    ATGCCTGGCA CAGCTCATGC CAACTGTGAT CAACCAGCTG CTCTGGTTCC CCGTGTGGAG
    ACTGTGTGTG AGCGTTGGAG GCTTGCACTC ATGAGTCACT ATAATGGTCA GCCGTCCTCT
    CAGGACTACA TGCCCCTCTG TGACGCTCAC GGAAATTTCC AGCCTGTGCA ATGTTATGGC
    AACAGCGCTT TCTGCTGGTG TGTGGACCGA CATGGTGTTG AAATTATTGG AACCCGGTCC
    AACGAGGATG TAAAGCCCCC CTGTATATCT GGTGATGCCG CCCCACTCAA CAATGCACTG
    ATGTATCCGG TTATAAGCTC CTCCCCTTCA GGCCCTGCTA TACTTTACAC ACAAGCCTCA
    CAGATTGGAG TCATTCCACT TGAAGAAACA GACCCAGTTC AGGAAAAGTC CACTGTGTTA
    TTAGCCTTAC AGGACTCGGT TGTGATTGGA ATTGACTATG ACTGTCAGGA GAACATCTTG
    TATTGGACAG ATTTTGGACG ACATACAATT TACAGAGCAG TTCTAATATC TGGGTCTGAA
    CCAGAGGCTG TTATCAGCAA AGACTTGATT CAGCCGGAGG GAATTGCAGT GGACTCTGTG
    CACAGGATGC TGTTCTGGGC AGACAGTGGG AAAGACAAGA TTGAGACATC TGGCCTGGAT
    GGCAGTAAAA GAAGAGTACT GATTGACTCA GGTTTGGTCA ATCCCAGAGC CATCATAGTA
    GACGCTAAGA GTGGAACTGT ATTCTGGACA GACTGGAACC GTGATGCTCC CAAAATAGAA
    AGCTCATCAC TGGAGGGACG TAACAGAAAA GTACTGGTGC AAGTGGGTCT CATGTTGCCC
    AATGCCTTGA CATGGGACAA TGCCACAAAT CAACTGTGCT GGGCTGATGC AGGCACAAAG
    AAACTGGAGT GCATATCTCC GGATGGGACT CAGAGACGTG TGTTCCAGGA TACTTTGAAC
    TATCCGTTCA GTCTAGCCAT CCATAACAAA CACTTCTATT ACACCGACTG GGAGAGAGAT
    GGGATACTGG TGATTGACCA AAGCACAGGG AAAAACACAG ATTATCTGCC CATTCAGCAG
    TCACACTTAT ATGGGATCAC ATTTATCCCA TCACAATGTC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329032.1
    CDS421..1785
    Translation

    Target ORF information:

    RefSeq Version XM_021473357.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nidogen 2b (osteonidogen) (nid2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCCCTCT GTTTTTCTTT AAAAAAAAAT TCTGCAGCAA AAAGGCCCAA GCCTTCATTA 
    ACTGCATGCC AGCAGCACAG AAACAGCCTG CAGGAGAGTA TGAGCATGTA TCCAGCTCTG
    GAGGCTTTTG TTCCAAAGTG TGATGATAAA GGCCAGTACA AACCCCTGCA GTGTTTGGGC
    TCCACTGGAC ACTGCTGGTG TGTGGACAGC AGGGGGCAGG AGAGAGTGGG CACCAGGACA
    ATGCCTGGCA CAGCTCATGC CAACTGTGAT CAACCAGCTG CTCTGGTTCC CCGTGTGGAG
    ACTGTGTGTG AGCGTTGGAG GCTTGCACTC ATGAGTCACT ATAATGGTCA GCCGTCCTCT
    CAGGACTACA TGCCCCTCTG TGACGCTCAC GGAAATTTCC AGCCTGTGCA ATGTTATGGC
    AACAGCGCTT TCTGCTGGTG TGTGGACCGA CATGGTGTTG AAATTATTGG AACCCGGTCC
    AACGAGGATG TAAAGCCCCC CTGTATATCT GGTGATGCCG CCCCACTCAA CAATGCACTG
    ATGTATCCGG TTATAAGCTC CTCCCCTTCA GGCCCTGCTA TACTTTACAC ACAAGCCTCA
    CAGATTGGAG TCATTCCACT TGAAGAAACA GACCCAGTTC AGGAAAAGTC CACTGTGTTA
    TTAGCCTTAC AGGACTCGGT TGTGATTGGA ATTGACTATG ACTGTCAGGA GAACATCTTG
    TATTGGACAG ATTTTGGACG ACATACAATT TACAGAGCAG TTCTAATATC TGGGTCTGAA
    CCAGAGGCTG TTATCAGCAA AGACTTGATT CAGCCGGAGG GAATTGCAGT GGACTCTGTG
    CACAGGATGC TGTTCTGGGC AGACAGTGGG AAAGACAAGA TTGAGACATC TGGCCTGGAT
    GGCAGTAAAA GAAGAGTACT GATTGACTCA GGTTTGGTCA ATCCCAGAGC CATCATAGTA
    GACGCTAAGA GTGGAACTGT ATTCTGGACA GACTGGAACC GTGATGCTCC CAAAATAGAA
    AGCTCATCAC TGGAGGGACG TAACAGAAAA GTACTGGTGC AAGTGGGTCT CATGTTGCCC
    AATGCCTTGA CATGGGACAA TGCCACAAAT CAACTGTGCT GGGCTGATGC AGGCACAAAG
    AAACTGGAGT GCATATCTCC GGATGGGACT CAGAGACGTG TGTTCCAGGA TACTTTGAAC
    TATCCGTTCA GTCTAGCCAT CCATAACAAA CACTTCTATT ACACCGACTG GGAGAGAGAT
    GGGATACTGG TGATTGACCA AAGCACAGGG AAAAACACAG ATTATCTGCC CATTCAGCAG
    TCACACTTAT ATGGGATCAC ATTTATCCCA TCACAATGTC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56747
    Clone ID Related Accession (Same CDS sequence) XM_017352728.1
    Accession Version XM_017352728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nidogen-2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGATTCAT TCAACCACTT GAATTTTGAC ATTAACATCA TTGGAGATTT ACCTAGCATT 
    CCCAGTGGAG CCAAAGTTCA TATTCTGCCA TATACAGAGA CCTATCACTA CAACCATTCA
    GTGTTGACAT CATCTTCACT GCGGGAGTAC ATGGTGGTGT CAGAAAATGG TGGATCTGAG
    ACGTTGTCCT ACCTACTACA GCAGAACATG AGTTTCAGTG GATGTGCACA TGATCCCAGG
    ACCACACCAG ACTTTCAGCA GGTGAACGTG GAGCATCTGA TGGTCATGTT CACAGAGGGA
    AACACACTCA GATATGCCAT CAGCAACAGA GTGGGACCTG TAGGAGGTGA GCTACCTGGA
    CTTGTAGAGT TGAATCCATG TTTAACTGGG AAGCACCATT GTGACCCGAT GGCCCTCTGT
    CTGCCAGGGG ACGGCACTCA GTATCTCTGT CAGTGTGCTA TGGGCTACCA GGGCGATGGA
    CAAAACTGCT ACGATGTGGA TGAGTGCGCG GAGGGGCTCA GTTCCTGTGG TCCTCATTCT
    GACTGTGTGA ACATGCCGGG TGGCCATCAC TGCCACTGTC AGTCTGGCTA CGAGTTTGAC
    CTCGATTTGC ATGAGTGTGT GGATATCGAT GAGTGTTTCG AGGAGCTCTG TCATCCGTTT
    GCTTCCTGCT CAAACACCCA GGGCTCGTTC CGCTGTCAAT GCTGGCCTGA ATACAAGGGC
    GATGGCTTCC TCTGCCAACC ACCACCAAAA AGGCCCAAGC CTTCATTAAC TGCATGCCAG
    CAGCACAGAA ACAGCCTGCA GGAGAGTATG AGCATGTATC CAGCTCTGGA GGCTTTTGTT
    CCAAAGTGTG ATGATAAAGG CCAGTACAAA CCCCTGCAGT GTTTGGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAGCAG GGGGCAGGAG AGAGTGGGCA CCAGGACAAT GCCTGGCACA
    GCTCATGCCA ACTGTGATCA ACCAGCTGCT CTGGTTCCCC GTGTGGAGAC TGTGTGTGAG
    CGTTGGAGGC TTGCACTCAT GAGTCACTAT AATGGTCAGC CGTCCTCTCA GGACTACATG
    CCCCTCTGTG ACGCTCACGG AAATTTCCAG CCTGTGCAAT GTTATGGCAA CAGCGCTTTC
    TGCTGGTGTG TGGACCGACA TGGTGTTGAA ATTATTGGAA CCCGGTCCAA CGAGGATGTA
    AAGCCCCCCT GTATATCTGG TGATGCCGCC CCACTCAACA ATGCACTGAT GTATCCGGTT
    ATAAGCTCCT CCCCTTCAGG CCCTGCTATA CTTTACACAC AAGCCTCACA GATTGGAGTC
    ATTCCACTTG AAGAAACAGA CCCAGTTCAG GAAAAGTCCA CTGTGTTATT AGCCTTACAG
    GACTCGGTTG TGATTGGAAT TGACTATGAC TGTCAGGAGA ACATCTTGTA TTGGACAGAT
    TTTGGACGAC ATACAATTTA CAGAGCAGTT CTAATATCTG GGTCTGAACC AGAGGCTGTT
    ATCAGCAAAG ACTTGATTCA GCCGGAGGGA ATTGCAGTGG ACTCTGTGCA CAGGATGCTG
    TTCTGGGCAG ACAGTGGGAA AGACAAGATT GAGACATCTG GCCTGGATGG CAGTAAAAGA
    AGAGTACTGA TTGACTCAGG TTTGGTCAAT CCCAGAGCCA TCATAGTAGA CGCTAAGAGT
    GGAACTGTAT TCTGGACAGA CTGGAACCGT GATGCTCCCA AAATAGAAAG CTCATCACTG
    GAGGGACGTA ACAGAAAAGT ACTGGTGCAA GTGGGTCTCA TGTTGCCCAA TGCCTTGACA
    TGGGACAATG CCACAAATCA ACTGTGCTGG GCTGATGCAG GCACAAAGAA ACTGGAGTGC
    ATATCTCCGG ATGGGACTCA GAGACGTGTG TTCCAGGATA CTTTGAACTA TCCGTTCAGT
    CTAGCCATCC ATAACAAACA CTTCTATTAC ACCGACTGGG AGAGAGATGG GATACTGGTG
    ATTGACCAAA GCACAGGGAA AAACACAGAT TATCTGCCCA TTCAGCAGTC ACACTTATAT
    GGGATCACAT TTATCCCATC ACAATGTCTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017208217.1
    CDS56..2128
    Translation

    Target ORF information:

    RefSeq Version XM_017352728.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio nidogen 2b (osteonidogen) (nid2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGATTCAT TCAACCACTT GAATTTTGAC ATTAACATCA TTGGAGATTT ACCTAGCATT 
    CCCAGTGGAG CCAAAGTTCA TATTCTGCCA TATACAGAGA CCTATCACTA CAACCATTCA
    GTGTTGACAT CATCTTCACT GCGGGAGTAC ATGGTGGTGT CAGAAAATGG TGGATCTGAG
    ACGTTGTCCT ACCTACTACA GCAGAACATG AGTTTCAGTG GATGTGCACA TGATCCCAGG
    ACCACACCAG ACTTTCAGCA GGTGAACGTG GAGCATCTGA TGGTCATGTT CACAGAGGGA
    AACACACTCA GATATGCCAT CAGCAACAGA GTGGGACCTG TAGGAGGTGA GCTACCTGGA
    CTTGTAGAGT TGAATCCATG TTTAACTGGG AAGCACCATT GTGACCCGAT GGCCCTCTGT
    CTGCCAGGGG ACGGCACTCA GTATCTCTGT CAGTGTGCTA TGGGCTACCA GGGCGATGGA
    CAAAACTGCT ACGATGTGGA TGAGTGCGCG GAGGGGCTCA GTTCCTGTGG TCCTCATTCT
    GACTGTGTGA ACATGCCGGG TGGCCATCAC TGCCACTGTC AGTCTGGCTA CGAGTTTGAC
    CTCGATTTGC ATGAGTGTGT GGATATCGAT GAGTGTTTCG AGGAGCTCTG TCATCCGTTT
    GCTTCCTGCT CAAACACCCA GGGCTCGTTC CGCTGTCAAT GCTGGCCTGA ATACAAGGGC
    GATGGCTTCC TCTGCCAACC ACCACCAAAA AGGCCCAAGC CTTCATTAAC TGCATGCCAG
    CAGCACAGAA ACAGCCTGCA GGAGAGTATG AGCATGTATC CAGCTCTGGA GGCTTTTGTT
    CCAAAGTGTG ATGATAAAGG CCAGTACAAA CCCCTGCAGT GTTTGGGCTC CACTGGACAC
    TGCTGGTGTG TGGACAGCAG GGGGCAGGAG AGAGTGGGCA CCAGGACAAT GCCTGGCACA
    GCTCATGCCA ACTGTGATCA ACCAGCTGCT CTGGTTCCCC GTGTGGAGAC TGTGTGTGAG
    CGTTGGAGGC TTGCACTCAT GAGTCACTAT AATGGTCAGC CGTCCTCTCA GGACTACATG
    CCCCTCTGTG ACGCTCACGG AAATTTCCAG CCTGTGCAAT GTTATGGCAA CAGCGCTTTC
    TGCTGGTGTG TGGACCGACA TGGTGTTGAA ATTATTGGAA CCCGGTCCAA CGAGGATGTA
    AAGCCCCCCT GTATATCTGG TGATGCCGCC CCACTCAACA ATGCACTGAT GTATCCGGTT
    ATAAGCTCCT CCCCTTCAGG CCCTGCTATA CTTTACACAC AAGCCTCACA GATTGGAGTC
    ATTCCACTTG AAGAAACAGA CCCAGTTCAG GAAAAGTCCA CTGTGTTATT AGCCTTACAG
    GACTCGGTTG TGATTGGAAT TGACTATGAC TGTCAGGAGA ACATCTTGTA TTGGACAGAT
    TTTGGACGAC ATACAATTTA CAGAGCAGTT CTAATATCTG GGTCTGAACC AGAGGCTGTT
    ATCAGCAAAG ACTTGATTCA GCCGGAGGGA ATTGCAGTGG ACTCTGTGCA CAGGATGCTG
    TTCTGGGCAG ACAGTGGGAA AGACAAGATT GAGACATCTG GCCTGGATGG CAGTAAAAGA
    AGAGTACTGA TTGACTCAGG TTTGGTCAAT CCCAGAGCCA TCATAGTAGA CGCTAAGAGT
    GGAACTGTAT TCTGGACAGA CTGGAACCGT GATGCTCCCA AAATAGAAAG CTCATCACTG
    GAGGGACGTA ACAGAAAAGT ACTGGTGCAA GTGGGTCTCA TGTTGCCCAA TGCCTTGACA
    TGGGACAATG CCACAAATCA ACTGTGCTGG GCTGATGCAG GCACAAAGAA ACTGGAGTGC
    ATATCTCCGG ATGGGACTCA GAGACGTGTG TTCCAGGATA CTTTGAACTA TCCGTTCAGT
    CTAGCCATCC ATAACAAACA CTTCTATTAC ACCGACTGGG AGAGAGATGG GATACTGGTG
    ATTGACCAAA GCACAGGGAA AAACACAGAT TATCTGCCCA TTCAGCAGTC ACACTTATAT
    GGGATCACAT TTATCCCATC ACAATGTCTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150674
    Clone ID Related Accession (Same CDS sequence) XM_021468530.1
    Accession Version XM_021468530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nidogen-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 21% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGAACT GCTGGATTTT AGCTGTACTT TTAACAAATG TTCGTTTTAT GAAAGCGATT 
    CAAAAGGAGA GACTTTTCCC TTTTGGAATA TCTGAAGGGG ATTCAGTTCT TCAGGAAGGT
    GACGATGAAG TGTCCGCAGC CCTGAAGATA AAAAAGCCAT TACAGTTCTA CGACATGCAG
    TTCAACATTC TTTATGTTTC CACAAATGGC TTTTTATCCC TTCAGCCTCC TCCTGAAGAG
    AGCCAGTATC TAGGAAGCAC CTTTCCTCTG AGTTTCTCTG CCATCGCACC TTTCCTGACA
    GACTTTAGTT CAGGAAGAGG GAAAGGATCT GTGTTTTACC GTGTAGAGGA GTCAATAAAT
    GTATTAAACC GGGCTTCTGA CATGGTTCAG CATGGTTTCA CTGGTTCTGC ATTCAAGCCC
    AGCCACACAG TCATCGCCAC ATGGGACAAC ATCGAATGTG AAGTGGAAAA GCTCCTTCAG
    GTGACCAGTT TCCAGGTTGT GTTGGCTGGT AATAAATCAG ATACATTTGC CTTGTTCCTG
    TATCCTGAGG ATGAGCAGCA AATGCTAAAT ATCAGTCACA ATTATGCTAA CATGTCAAAA
    GGAGATGTCA AACTGCAGGC TGGTTTTAAT AGAGGCGATA CATCGACCAC AGGAGAGCTG
    TTTTACAGTC TGCCTGGCTC TAAAGAGAAC ATCAACCTCT ATAGGGAGGG GAACATGGGA
    ATAAGCGGAC TGTGGATTTT CCACATCGGA TCCAGCCGCT TCCCTTTTCA GAATATAGTT
    CCTGCTTCTG TCACCAAGTC TGACTCTATG GGTTATAAAA CCACTCAAAG AACCACAAAA
    TCAAATACAA CTGCAGATGA ATCTGCTTCT GAGCCTCAGG TGGACAATCC AGCTTCACTT
    GTTCAACAGC CCTTAAAACC AAACCAAGAA CTACAACAAG TCAATGTAAC CAAAATGTGT
    GGTTTGCACC TGAGATTCTG CTCTGTGGAT GGATACTGCA CAGACTACCC AACTGGGCCA
    TGCTGTTACT GCAGAACCGG CTACTATGGC AATGGCAGAC ATTGTTTACC CATGGGTATT
    TCTCAACCTT TGAGTGGAAA GCTGAGTGGC CATGTTTCTG TTGGGGGCAC TAAAGTTCAG
    CTAAACAGTG TGGACCTCCA TGGCTTTGCG GTGGTGGGAG AAGGTCGTGT GTATGTCTCC
    ATCAGTCCTG TCCCAGCTAG GGTGGGCTGG GCTCTGATGG CTGTGGGCCC GCTGGTCTCC
    ACATTTGGCT GGCTCTTTGC TCTTGAGCTC CAGAACCATC CAAATGGCTT CAGCATAACT
    GGAGCTGAGT TTTTCTACAA AGCAGAGCTG GTGTTCAGCC CACAAGGACA GCGTGTTGTC
    ATTATTCAAG AGGCTCAAGG AATGGATTCA TTCAACCACT TGAATTTTGA CATTAACATC
    ATTGGAGATT TACCTAGCAT TCCCAGTGGA GCCAAAGTTC ATATTCTGCC ATATACAGAG
    ACCTATCACT ACAACCATTC AGTGTTGACA TCATCTTCAC TGCGGGAGTA CATGGTGGTG
    TCAGAAAATG GTGGATCTGA GACGTTGTCC TACCTACTAC AGCAGAACAT GAGTTTCAGT
    GGATGTGCAC ATGATCCCAG GACCACACCA GACTTTCAGC AGGTGAACGT GGAGCATCTG
    ATGGTCATGT TCACAGAGGG AAACACACTC AGATATGCCA TCAGCAACAG AGTGGGACCT
    GTAGGAGGTG AGCTACCTGG ACTTGTAGAG TTGAATCCAT GTTTAACTGG GAAGCACCAT
    TGTGACCCGA TGGCCCTCTG TCTGCCAGGG GACGGCACTC AGTATCTCTG TCAGTGTGCT
    ATGGGCTACC AGGGCGATGG ACAAAACTGC TACGATGTGG ATGAGTGCGC GGAGGGGCTC
    AGTTCCTGTG GTCCTCATTC TGACTGTGTG AACATGCCGG GTGGCCATCA CTGCCACTGT
    CAGTCTGGCT ACGAGTTTGA CCTCGATTTG CATGAGTGTG TGGATATCGA TGAGTGTTTC
    GAGGAGCTCT GTCATCCGTT TGCTTCCTGC TCAAACACCC AGGGCTCGTT CCGCTGTCAA
    TGCTGGCCTG AATACAAGGG CGATGGCTTC CTCTGCCAAC CACCACGTTT TTCTTTAAAA
    AAAAATTCTG CAGCAAAAAG GCCCAAGCCT TCATTAACTG CATGCCAGCA GCACAGAAAC
    AGCCTGCAGG AGAGTATGAG CATGTATCCA GCTCTGGAGG CTTTTGTTCC AAAGTGTGAT
    GATAAAGGCC AGTACAAACC CCTGCAGTGT TTGGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGCAGGG GGCAGGAGAG AGTGGGCACC AGGACAATGC CTGGCACAGC TCATGCCAAC
    TGTGATCAAC CAGCTGCTCT GGTTCCCCGT GTGGAGACTG TGTGTGAGCG TTGGAGGCTT
    GCACTCATGA GTCACTATAA TGGTCAGCCG TCCTCTCAGG ACTACATGCC CCTCTGTGAC
    GCTCACGGAA ATTTCCAGCC TGTGCAATGT TATGGCAACA GCGCTTTCTG CTGGTGTGTG
    GACCGACATG GTGTTGAAAT TATTGGAACC CGGTCCAACG AGGATGTAAA GCCCCCCTGT
    ATATCTGGTG ATGCCGCCCC ACTCAACAAT GCACTGATGT ATCCGGTTAT AAGCTCCTCC
    CCTTCAGGCC CTGCTATACT TTACACACAA GCCTCACAGA TTGGAGTCAT TCCACTTGAA
    GAAACAGACC CAGTTCAGGA AAAGTCCACT GTGTTATTAG CCTTACAGGA CTCGGTTGTG
    ATTGGAATTG ACTATGACTG TCAGGAGAAC ATCTTGTATT GGACAGATTT TGGACGACAT
    ACAATTTACA GAGCAGTTCT AATATCTGGG TCTGAACCAG AGGCTGTTAT CAGCAAAGAC
    TTGATTCAGC CGGAGGGAAT TGCAGTGGAC TCTGTGCACA GGATGCTGTT CTGGGCAGAC
    AGTGGGAAAG ACAAGATTGA GACATCTGGC CTGGATGGCA GTAAAAGAAG AGTACTGATT
    GACTCAGGTT TGGTCAATCC CAGAGCCATC ATAGTAGACG CTAAGAGTGG AACTGTATTC
    TGGACAGACT GGAACCGTGA TGCTCCCAAA ATAGAAAGCT CATCACTGGA GGGACGTAAC
    AGAAAAGTAC TGGTGCAAGT GGGTCTCATG TTGCCCAATG CCTTGACATG GGACAATGCC
    ACAAATCAAC TGTGCTGGGC TGATGCAGGC ACAAAGAAAC TGGAGTGCAT ATCTCCGGAT
    GGGACTCAGA GACGTGTGTT CCAGGATACT TTGAACTATC CGTTCAGTCT AGCCATCCAT
    AACAAACACT TCTATTACAC CGACTGGGAG AGAGATGGGA TACTGGTGAT TGACCAAAGC
    ACAGGGAAAA ACACAGATTA TCTGCCCATT CAGCAGTCAC ACTTATATGG GATCACATTT
    ATCCCATCAC AATGTCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324205.1
    CDS98..3598
    Translation

    Target ORF information:

    RefSeq Version XM_021468530.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nidogen 2b (osteonidogen) (nid2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGAACT GCTGGATTTT AGCTGTACTT TTAACAAATG TTCGTTTTAT GAAAGCGATT 
    CAAAAGGAGA GACTTTTCCC TTTTGGAATA TCTGAAGGGG ATTCAGTTCT TCAGGAAGGT
    GACGATGAAG TGTCCGCAGC CCTGAAGATA AAAAAGCCAT TACAGTTCTA CGACATGCAG
    TTCAACATTC TTTATGTTTC CACAAATGGC TTTTTATCCC TTCAGCCTCC TCCTGAAGAG
    AGCCAGTATC TAGGAAGCAC CTTTCCTCTG AGTTTCTCTG CCATCGCACC TTTCCTGACA
    GACTTTAGTT CAGGAAGAGG GAAAGGATCT GTGTTTTACC GTGTAGAGGA GTCAATAAAT
    GTATTAAACC GGGCTTCTGA CATGGTTCAG CATGGTTTCA CTGGTTCTGC ATTCAAGCCC
    AGCCACACAG TCATCGCCAC ATGGGACAAC ATCGAATGTG AAGTGGAAAA GCTCCTTCAG
    GTGACCAGTT TCCAGGTTGT GTTGGCTGGT AATAAATCAG ATACATTTGC CTTGTTCCTG
    TATCCTGAGG ATGAGCAGCA AATGCTAAAT ATCAGTCACA ATTATGCTAA CATGTCAAAA
    GGAGATGTCA AACTGCAGGC TGGTTTTAAT AGAGGCGATA CATCGACCAC AGGAGAGCTG
    TTTTACAGTC TGCCTGGCTC TAAAGAGAAC ATCAACCTCT ATAGGGAGGG GAACATGGGA
    ATAAGCGGAC TGTGGATTTT CCACATCGGA TCCAGCCGCT TCCCTTTTCA GAATATAGTT
    CCTGCTTCTG TCACCAAGTC TGACTCTATG GGTTATAAAA CCACTCAAAG AACCACAAAA
    TCAAATACAA CTGCAGATGA ATCTGCTTCT GAGCCTCAGG TGGACAATCC AGCTTCACTT
    GTTCAACAGC CCTTAAAACC AAACCAAGAA CTACAACAAG TCAATGTAAC CAAAATGTGT
    GGTTTGCACC TGAGATTCTG CTCTGTGGAT GGATACTGCA CAGACTACCC AACTGGGCCA
    TGCTGTTACT GCAGAACCGG CTACTATGGC AATGGCAGAC ATTGTTTACC CATGGGTATT
    TCTCAACCTT TGAGTGGAAA GCTGAGTGGC CATGTTTCTG TTGGGGGCAC TAAAGTTCAG
    CTAAACAGTG TGGACCTCCA TGGCTTTGCG GTGGTGGGAG AAGGTCGTGT GTATGTCTCC
    ATCAGTCCTG TCCCAGCTAG GGTGGGCTGG GCTCTGATGG CTGTGGGCCC GCTGGTCTCC
    ACATTTGGCT GGCTCTTTGC TCTTGAGCTC CAGAACCATC CAAATGGCTT CAGCATAACT
    GGAGCTGAGT TTTTCTACAA AGCAGAGCTG GTGTTCAGCC CACAAGGACA GCGTGTTGTC
    ATTATTCAAG AGGCTCAAGG AATGGATTCA TTCAACCACT TGAATTTTGA CATTAACATC
    ATTGGAGATT TACCTAGCAT TCCCAGTGGA GCCAAAGTTC ATATTCTGCC ATATACAGAG
    ACCTATCACT ACAACCATTC AGTGTTGACA TCATCTTCAC TGCGGGAGTA CATGGTGGTG
    TCAGAAAATG GTGGATCTGA GACGTTGTCC TACCTACTAC AGCAGAACAT GAGTTTCAGT
    GGATGTGCAC ATGATCCCAG GACCACACCA GACTTTCAGC AGGTGAACGT GGAGCATCTG
    ATGGTCATGT TCACAGAGGG AAACACACTC AGATATGCCA TCAGCAACAG AGTGGGACCT
    GTAGGAGGTG AGCTACCTGG ACTTGTAGAG TTGAATCCAT GTTTAACTGG GAAGCACCAT
    TGTGACCCGA TGGCCCTCTG TCTGCCAGGG GACGGCACTC AGTATCTCTG TCAGTGTGCT
    ATGGGCTACC AGGGCGATGG ACAAAACTGC TACGATGTGG ATGAGTGCGC GGAGGGGCTC
    AGTTCCTGTG GTCCTCATTC TGACTGTGTG AACATGCCGG GTGGCCATCA CTGCCACTGT
    CAGTCTGGCT ACGAGTTTGA CCTCGATTTG CATGAGTGTG TGGATATCGA TGAGTGTTTC
    GAGGAGCTCT GTCATCCGTT TGCTTCCTGC TCAAACACCC AGGGCTCGTT CCGCTGTCAA
    TGCTGGCCTG AATACAAGGG CGATGGCTTC CTCTGCCAAC CACCACGTTT TTCTTTAAAA
    AAAAATTCTG CAGCAAAAAG GCCCAAGCCT TCATTAACTG CATGCCAGCA GCACAGAAAC
    AGCCTGCAGG AGAGTATGAG CATGTATCCA GCTCTGGAGG CTTTTGTTCC AAAGTGTGAT
    GATAAAGGCC AGTACAAACC CCTGCAGTGT TTGGGCTCCA CTGGACACTG CTGGTGTGTG
    GACAGCAGGG GGCAGGAGAG AGTGGGCACC AGGACAATGC CTGGCACAGC TCATGCCAAC
    TGTGATCAAC CAGCTGCTCT GGTTCCCCGT GTGGAGACTG TGTGTGAGCG TTGGAGGCTT
    GCACTCATGA GTCACTATAA TGGTCAGCCG TCCTCTCAGG ACTACATGCC CCTCTGTGAC
    GCTCACGGAA ATTTCCAGCC TGTGCAATGT TATGGCAACA GCGCTTTCTG CTGGTGTGTG
    GACCGACATG GTGTTGAAAT TATTGGAACC CGGTCCAACG AGGATGTAAA GCCCCCCTGT
    ATATCTGGTG ATGCCGCCCC ACTCAACAAT GCACTGATGT ATCCGGTTAT AAGCTCCTCC
    CCTTCAGGCC CTGCTATACT TTACACACAA GCCTCACAGA TTGGAGTCAT TCCACTTGAA
    GAAACAGACC CAGTTCAGGA AAAGTCCACT GTGTTATTAG CCTTACAGGA CTCGGTTGTG
    ATTGGAATTG ACTATGACTG TCAGGAGAAC ATCTTGTATT GGACAGATTT TGGACGACAT
    ACAATTTACA GAGCAGTTCT AATATCTGGG TCTGAACCAG AGGCTGTTAT CAGCAAAGAC
    TTGATTCAGC CGGAGGGAAT TGCAGTGGAC TCTGTGCACA GGATGCTGTT CTGGGCAGAC
    AGTGGGAAAG ACAAGATTGA GACATCTGGC CTGGATGGCA GTAAAAGAAG AGTACTGATT
    GACTCAGGTT TGGTCAATCC CAGAGCCATC ATAGTAGACG CTAAGAGTGG AACTGTATTC
    TGGACAGACT GGAACCGTGA TGCTCCCAAA ATAGAAAGCT CATCACTGGA GGGACGTAAC
    AGAAAAGTAC TGGTGCAAGT GGGTCTCATG TTGCCCAATG CCTTGACATG GGACAATGCC
    ACAAATCAAC TGTGCTGGGC TGATGCAGGC ACAAAGAAAC TGGAGTGCAT ATCTCCGGAT
    GGGACTCAGA GACGTGTGTT CCAGGATACT TTGAACTATC CGTTCAGTCT AGCCATCCAT
    AACAAACACT TCTATTACAC CGACTGGGAG AGAGATGGGA TACTGGTGAT TGACCAAAGC
    ACAGGGAAAA ACACAGATTA TCTGCCCATT CAGCAGTCAC ACTTATATGG GATCACATTT
    ATCCCATCAC AATGTCTATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.