si:dkey-11f4.7 cDNA ORF clone, Danio rerio(zebrafish)

The following si:dkey-11f4.7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-11f4.7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144017 XM_021478800.1
Latest version!
Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa144018 XM_021478801.1
Latest version!
Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa45827 XM_009304390.3
Latest version!
Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa45828 XM_009304391.2
Latest version!
Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
ODa45827 XM_009304390.2 Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144017
    Clone ID Related Accession (Same CDS sequence) XM_021478800.1
    Accession Version XM_021478800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7905bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGTACA CACTCCTCAC ACACACACTC AAACATGGAG TCTTTCTCAT GATTCTCTCT
    GCTCATGTCA TCTGTTTTCT GCTGTCTGTC AGGCTGCTGG AGAATCTGCT GCCCAAGTAT
    ATCAGAGGAG CGTCTGGACC CAATGCTAAA GTGGCGCATC GACTGCAAGT GGAGTTCTCC
    AGAGAGCCGG AGAATGCGGA TCTGCAGTTC TTGCGTCCTC TGTCAGTGAG TCTCCAGCAG
    GGGAACCGCA GCTCCCCAGA CAGCGCCCCG CAGTGGTGGG TGGTGCAGGA GTGCTGGCCC
    GGACCTCCAC CCAGCAGCTG CCGCAGTATA GAGATGGTGG TGTTCAATGA TAAAGCCAGC
    CCGCCCAGCG TGGGCTTCCT GGCCGGATAC GGTATCGTGG GTCTGTACAT GTCCGTGGTG
    CTGGTCATCG GGAAGTTCGT GCGGGAGTTC TTCACAGGGA TCTCTCGGTC CATCATGTAC
    GAGGAGCTGC CGTGTGTGGA CCGCGTGCTC AAACTCTGCA ACGACATCTT CATAGTGCGC
    GAGGCGGGAG AGATGGAGAT GGAGGAGCAG ATGTTCGAGA AGCTCATCTT CCTCTACCGC
    TCTCCCGAAA CCATGATCAA GATGACCCAC AACAAGAGCG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334475.1
    CDS234..8138
    Translation

    Target ORF information:

    RefSeq Version XM_021478800.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGTACA CACTCCTCAC ACACACACTC AAACATGGAG TCTTTCTCAT GATTCTCTCT
    GCTCATGTCA TCTGTTTTCT GCTGTCTGTC AGGCTGCTGG AGAATCTGCT GCCCAAGTAT
    ATCAGAGGAG CGTCTGGACC CAATGCTAAA GTGGCGCATC GACTGCAAGT GGAGTTCTCC
    AGAGAGCCGG AGAATGCGGA TCTGCAGTTC TTGCGTCCTC TGTCAGTGAG TCTCCAGCAG
    GGGAACCGCA GCTCCCCAGA CAGCGCCCCG CAGTGGTGGG TGGTGCAGGA GTGCTGGCCC
    GGACCTCCAC CCAGCAGCTG CCGCAGTATA GAGATGGTGG TGTTCAATGA TAAAGCCAGC
    CCGCCCAGCG TGGGCTTCCT GGCCGGATAC GGTATCGTGG GTCTGTACAT GTCCGTGGTG
    CTGGTCATCG GGAAGTTCGT GCGGGAGTTC TTCACAGGGA TCTCTCGGTC CATCATGTAC
    GAGGAGCTGC CGTGTGTGGA CCGCGTGCTC AAACTCTGCA ACGACATCTT CATAGTGCGC
    GAGGCGGGAG AGATGGAGAT GGAGGAGCAG ATGTTCGAGA AGCTCATCTT CCTCTACCGC
    TCTCCCGAAA CCATGATCAA GATGACCCAC AACAAGAGCG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144018
    Clone ID Related Accession (Same CDS sequence) XM_021478801.1
    Accession Version XM_021478801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGA TGGGCATCAG ATGAGGAAGA AGCGTTCACA GAAGAAGAGA
    AGCGTGCCGC TGAGCAGGAA GGAGAGAATG AAGAAATACA TGAATGACCG ACTCCTGGAG
    GCCAAAGCTG CGGTCATAGA GCTTGCTCTA CACATATACT TACCCATAAG GCAGTTCTTC
    TATGACATCA TCCACCCCGA GTACAGCCCT GTGTGTGACG TGTACGCGCT GATGTTCCTG
    ATAGACGTGG TCAACTTCAT CATCATTATA TTCGGATACT GGGCTTTTGG AAAGCACAGT
    GCGGCGGCTG ATATAACAGA GTCGCTGTCT GAAGACCAGG TTCCCGAGGC GTTCCTCGTC
    ATGCTCCTCA TCCAGTTCAG CACCATGATC GTGGACCGAG CGCTCTACCT GCGCAAAACC
    CTGCTGGGGA AGTGTGTGTT CCAGGTGGTG CTCGTCTTCG GGATACACTT CTGGATGTTC
    TTCATTTTGC CCGGTGTCAC TGAGAGGAGA TTCAACAGGA ACCGAGTTGC TCAGCTGTGG
    TACTTTGTAA AGTGCATCTA CTTTGGCCTC TCGGCGTATC AGATCAAGTG TGGATACCCA
    AACCGTGTGC TCGGAAACTT CCTCACCAAG AACCACAACT ACTTCAACCT CTTCCTCTTT
    CAGGGGTTCC GATTGGTTCC GTTCCTAACA GAGTTGCGGG CCGTAATGGA CTGGGTGTGG
    ACGGACACCA CTCTCAGCCT GTCCAACTGG ATCTGTGTGG AGGACATCTA CGCCAACATC
    TTCATCCTCA AGTGCTGGAG AGAAGCAGAA AAGAAATATC CCCATCGGCG TGGACAGCGG
    AAGAAGAAGG TGGTGAAGTA CACCATGGGC GGCTTCATCA TTTTTGCTCT GATCTGCATC
    GTCTGGTTCC CGCTGCTCTT CATGTCATTG GTTAAATCTG TCGCAGGAGT GACCAATCAG
    CCTTTGGACG TGTCCATCCA GCTGAGCATT CTGGGATACG AGCCGTTGTT CAGCATGAGT
    GCGCAGGAGC ACAATCTGAT CCCATACTCT CAATCAGCCT TCCAGAAGAT GACCAATCGC
    TTCGCCACGT ACCCGTCTGC CATGCAGTTC ATCGTGAACT ATATGGCAGA AGACATCGTG
    ACGGCGAAGA TCAAGAGCGA CGCCAGTCTG CTGTGGAGCG TCAGTCCTGC CAGCCGAGAC
    GCCATGATCT CTGAGCTCAG CAACTCCACA CACTTCTACA TCACACTGCG CTGGACTTTA
    CTACGAAATG CCTCTGTGTC GATGAATGCG GAGACGGCTG GTGAACACAC AGTGAAGTAT
    GAAGACCGTG CGCTGAGAGA CCAGCTGGTG GCGATGCTCA GAGGAACACA CACTCGCCCG
    CTGTACACAC TCCTCACACA CACACTCAAA CATGGAGTCT TTCTCATGAT TCTCTCTGCT
    CATGTCATCT GTTTTCTGCT GTCTGTCAGG CTGCTGGAGA ATCTGCTGCC CAAGTATATC
    AGAGGAGCGT CTGGACCCAA TGCTAAAGTG GCGCATCGAC TGCAAGTGGA GTTCTCCAGA
    GAGCCGGAGA ATGCGGATCT GCAGTTCTTG CGTCCTCTGT CAGTGAGTCT CCAGCAGGGG
    AACCGCAGCT CCCCAGACAG CGCCCCGCAG TGGTGGGTGG TGCAGGAGTG CTGGCCCGGA
    CCTCCACCCA GCAGCTGCCG CAGTATAGAG ATGGTGGTGT TCAATGATAA AGCCAGCCCG
    CCCAGCGTGG GCTTCCTGGC CGGATACGGT ATCGTGGGTC TGTACATGTC CGTGGTGCTG
    GTCATCGGGA AGTTCGTGCG GGAGTTCTTC ACAGGGATCT CTCGGTCCAT CATGTACGAG
    GAGCTGCCGT GTGTGGACCG CGTGCTCAAA CTCTGCAACG ACATCTTCAT AGTGCGCGAG
    GCGGGAGAGA TGGAGATGGA GGAGCAGATG TTCGAGAAGC TCATCTTCCT CTACCGCTCT
    CCCGAAACCA TGATCAAGAT GACCCACAAC AAGAGCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334476.1
    CDS234..8135
    Translation

    Target ORF information:

    RefSeq Version XM_021478801.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGA TGGGCATCAG ATGAGGAAGA AGCGTTCACA GAAGAAGAGA
    AGCGTGCCGC TGAGCAGGAA GGAGAGAATG AAGAAATACA TGAATGACCG ACTCCTGGAG
    GCCAAAGCTG CGGTCATAGA GCTTGCTCTA CACATATACT TACCCATAAG GCAGTTCTTC
    TATGACATCA TCCACCCCGA GTACAGCCCT GTGTGTGACG TGTACGCGCT GATGTTCCTG
    ATAGACGTGG TCAACTTCAT CATCATTATA TTCGGATACT GGGCTTTTGG AAAGCACAGT
    GCGGCGGCTG ATATAACAGA GTCGCTGTCT GAAGACCAGG TTCCCGAGGC GTTCCTCGTC
    ATGCTCCTCA TCCAGTTCAG CACCATGATC GTGGACCGAG CGCTCTACCT GCGCAAAACC
    CTGCTGGGGA AGTGTGTGTT CCAGGTGGTG CTCGTCTTCG GGATACACTT CTGGATGTTC
    TTCATTTTGC CCGGTGTCAC TGAGAGGAGA TTCAACAGGA ACCGAGTTGC TCAGCTGTGG
    TACTTTGTAA AGTGCATCTA CTTTGGCCTC TCGGCGTATC AGATCAAGTG TGGATACCCA
    AACCGTGTGC TCGGAAACTT CCTCACCAAG AACCACAACT ACTTCAACCT CTTCCTCTTT
    CAGGGGTTCC GATTGGTTCC GTTCCTAACA GAGTTGCGGG CCGTAATGGA CTGGGTGTGG
    ACGGACACCA CTCTCAGCCT GTCCAACTGG ATCTGTGTGG AGGACATCTA CGCCAACATC
    TTCATCCTCA AGTGCTGGAG AGAAGCAGAA AAGAAATATC CCCATCGGCG TGGACAGCGG
    AAGAAGAAGG TGGTGAAGTA CACCATGGGC GGCTTCATCA TTTTTGCTCT GATCTGCATC
    GTCTGGTTCC CGCTGCTCTT CATGTCATTG GTTAAATCTG TCGCAGGAGT GACCAATCAG
    CCTTTGGACG TGTCCATCCA GCTGAGCATT CTGGGATACG AGCCGTTGTT CAGCATGAGT
    GCGCAGGAGC ACAATCTGAT CCCATACTCT CAATCAGCCT TCCAGAAGAT GACCAATCGC
    TTCGCCACGT ACCCGTCTGC CATGCAGTTC ATCGTGAACT ATATGGCAGA AGACATCGTG
    ACGGCGAAGA TCAAGAGCGA CGCCAGTCTG CTGTGGAGCG TCAGTCCTGC CAGCCGAGAC
    GCCATGATCT CTGAGCTCAG CAACTCCACA CACTTCTACA TCACACTGCG CTGGACTTTA
    CTACGAAATG CCTCTGTGTC GATGAATGCG GAGACGGCTG GTGAACACAC AGTGAAGTAT
    GAAGACCGTG CGCTGAGAGA CCAGCTGGTG GCGATGCTCA GAGGAACACA CACTCGCCCG
    CTGTACACAC TCCTCACACA CACACTCAAA CATGGAGTCT TTCTCATGAT TCTCTCTGCT
    CATGTCATCT GTTTTCTGCT GTCTGTCAGG CTGCTGGAGA ATCTGCTGCC CAAGTATATC
    AGAGGAGCGT CTGGACCCAA TGCTAAAGTG GCGCATCGAC TGCAAGTGGA GTTCTCCAGA
    GAGCCGGAGA ATGCGGATCT GCAGTTCTTG CGTCCTCTGT CAGTGAGTCT CCAGCAGGGG
    AACCGCAGCT CCCCAGACAG CGCCCCGCAG TGGTGGGTGG TGCAGGAGTG CTGGCCCGGA
    CCTCCACCCA GCAGCTGCCG CAGTATAGAG ATGGTGGTGT TCAATGATAA AGCCAGCCCG
    CCCAGCGTGG GCTTCCTGGC CGGATACGGT ATCGTGGGTC TGTACATGTC CGTGGTGCTG
    GTCATCGGGA AGTTCGTGCG GGAGTTCTTC ACAGGGATCT CTCGGTCCAT CATGTACGAG
    GAGCTGCCGT GTGTGGACCG CGTGCTCAAA CTCTGCAACG ACATCTTCAT AGTGCGCGAG
    GCGGGAGAGA TGGAGATGGA GGAGCAGATG TTCGAGAAGC TCATCTTCCT CTACCGCTCT
    CCCGAAACCA TGATCAAGAT GACCCACAAC AAGAGCGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45827
    Clone ID Related Accession (Same CDS sequence) XM_009304390.2 , XM_009304390.3
    Accession Version XM_009304390.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGCTGC TGGAGAATCT GCTGCCCAAG TATATCAGAG GAGCGTCTGG ACCCAATGCT
    AAAGTGGCGC ATCGACTGCA AGTGGAGTTC TCCAGAGAGC CGGAGAATGC GGATCTGCAG
    TTCTTGCGTC CTCTGTCAGT GAGTCTCCAG CAGGGGAACC GCAGCTCCCC AGACAGCGCC
    CCGCAGTGGT GGGTGGTGCA GGAGTGCTGG CCCGGACCTC CACCCAGCAG CTGCCGCAGT
    ATAGAGATGG TGGTGTTCAA TGATAAAGCC AGCCCGCCCA GCGTGGGCTT CCTGGCCGGA
    TACGGTATCG TGGGTCTGTA CATGTCCGTG GTGCTGGTCA TCGGGAAGTT CGTGCGGGAG
    TTCTTCACAG GGATCTCTCG GTCCATCATG TACGAGGAGC TGCCGTGTGT GGACCGCGTG
    CTCAAACTCT GCAACGACAT CTTCATAGTG CGCGAGGCGG GAGAGATGGA GATGGAGGAG
    CAGATGTTCG AGAAGCTCAT CTTCCTCTAC CGCTCTCCCG AAACCATGAT CAAGATGACC
    CACAACAAGA GCGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302665.1
    CDS156..7973
    Translation

    Target ORF information:

    RefSeq Version XM_009304390.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304390.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGCTGC TGGAGAATCT GCTGCCCAAG TATATCAGAG GAGCGTCTGG ACCCAATGCT
    AAAGTGGCGC ATCGACTGCA AGTGGAGTTC TCCAGAGAGC CGGAGAATGC GGATCTGCAG
    TTCTTGCGTC CTCTGTCAGT GAGTCTCCAG CAGGGGAACC GCAGCTCCCC AGACAGCGCC
    CCGCAGTGGT GGGTGGTGCA GGAGTGCTGG CCCGGACCTC CACCCAGCAG CTGCCGCAGT
    ATAGAGATGG TGGTGTTCAA TGATAAAGCC AGCCCGCCCA GCGTGGGCTT CCTGGCCGGA
    TACGGTATCG TGGGTCTGTA CATGTCCGTG GTGCTGGTCA TCGGGAAGTT CGTGCGGGAG
    TTCTTCACAG GGATCTCTCG GTCCATCATG TACGAGGAGC TGCCGTGTGT GGACCGCGTG
    CTCAAACTCT GCAACGACAT CTTCATAGTG CGCGAGGCGG GAGAGATGGA GATGGAGGAG
    CAGATGTTCG AGAAGCTCAT CTTCCTCTAC CGCTCTCCCG AAACCATGAT CAAGATGACC
    CACAACAAGA GCGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45827
    Clone ID Related Accession (Same CDS sequence) XM_009304390.2 , XM_009304390.3
    Accession Version XM_009304390.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGCTGC TGGAGAATCT GCTGCCCAAG TATATCAGAG GAGCGTCTGG ACCCAATGCT
    AAAGTGGCGC ATCGACTGCA AGTGGAGTTC TCCAGAGAGC CGGAGAATGC GGATCTGCAG
    TTCTTGCGTC CTCTGTCAGT GAGTCTCCAG CAGGGGAACC GCAGCTCCCC AGACAGCGCC
    CCGCAGTGGT GGGTGGTGCA GGAGTGCTGG CCCGGACCTC CACCCAGCAG CTGCCGCAGT
    ATAGAGATGG TGGTGTTCAA TGATAAAGCC AGCCCGCCCA GCGTGGGCTT CCTGGCCGGA
    TACGGTATCG TGGGTCTGTA CATGTCCGTG GTGCTGGTCA TCGGGAAGTT CGTGCGGGAG
    TTCTTCACAG GGATCTCTCG GTCCATCATG TACGAGGAGC TGCCGTGTGT GGACCGCGTG
    CTCAAACTCT GCAACGACAT CTTCATAGTG CGCGAGGCGG GAGAGATGGA GATGGAGGAG
    CAGATGTTCG AGAAGCTCAT CTTCCTCTAC CGCTCTCCCG AAACCATGAT CAAGATGACC
    CACAACAAGA GCGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302665.1
    CDS234..8051
    Translation

    Target ORF information:

    RefSeq Version XM_009304390.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304390.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGC AGATGGGCAT CAGATGAGGA AGAAGCGTTC ACAGAAGAAG
    AGAAGCGTGC CGCTGAGCAG GAAGGAGAGA ATGAAGAAAT ACATGAATGA CCGACTCCTG
    GAGGCCAAAG CTGCGGTCAT AGAGCTTGCT CTACACATAT ACTTACCCAT AAGGCAGTTC
    TTCTATGACA TCATCCACCC CGAGTACAGC CCTGTGTGTG ACGTGTACGC GCTGATGTTC
    CTGATAGACG TGGTCAACTT CATCATCATT ATATTCGGAT ACTGGGCTTT TGGAAAGCAC
    AGTGCGGCGG CTGATATAAC AGAGTCGCTG TCTGAAGACC AGGTTCCCGA GGCGTTCCTC
    GTCATGCTCC TCATCCAGTT CAGCACCATG ATCGTGGACC GAGCGCTCTA CCTGCGCAAA
    ACCCTGCTGG GGAAGTGTGT GTTCCAGGTG GTGCTCGTCT TCGGGATACA CTTCTGGATG
    TTCTTCATTT TGCCCGGTGT CACTGAGAGG AGATTCAACA GGAACCGAGT TGCTCAGCTG
    TGGTACTTTG TAAAGTGCAT CTACTTTGGC CTCTCGGCGT ATCAGATCAA GTGTGGATAC
    CCAAACCGTG TGCTCGGAAA CTTCCTCACC AAGAACCACA ACTACTTCAA CCTCTTCCTC
    TTTCAGGGGT TCCGATTGGT TCCGTTCCTA ACAGAGTTGC GGGCCGTAAT GGACTGGGTG
    TGGACGGACA CCACTCTCAG CCTGTCCAAC TGGATCTGTG TGGAGGACAT CTACGCCAAC
    ATCTTCATCC TCAAGTGCTG GAGAGAAGCA GAAAAGAAAT ATCCCCATCG GCGTGGACAG
    CGGAAGAAGA AGGTGGTGAA GTACACCATG GGCGGCTTCA TCATTTTTGC TCTGATCTGC
    ATCGTCTGGT TCCCGCTGCT CTTCATGTCA TTGGTTAAAT CTGTCGCAGG AGTGACCAAT
    CAGCCTTTGG ACGTGTCCAT CCAGCTGAGC ATTCTGGGAT ACGAGCCGTT GTTCAGCATG
    AGTGCGCAGG AGCACAATCT GATCCCATAC TCTCAATCAG CCTTCCAGAA GATGACCAAT
    CGCTTCGCCA CGTACCCGTC TGCCATGCAG TTCATCGTGA ACTATATGGC AGAAGACATC
    GTGACGGCGA AGATCAAGAG CGACGCCAGT CTGCTGTGGA GCGTCAGTCC TGCCAGCCGA
    GACGCCATGA TCTCTGAGCT CAGCAACTCC ACACACTTCT ACATCACACT GCGCTGGACT
    TTACTACGAA ATGCCTCTGT GTCGATGAAT GCGGAGACGG CTGGTGAACA CACAGTGAAG
    TATGAAGACC GTGCGCTGAG AGACCAGCTG GTGGCGATGC TCAGAGGAAC ACACACTCGC
    CCGCTGCTGC TGGAGAATCT GCTGCCCAAG TATATCAGAG GAGCGTCTGG ACCCAATGCT
    AAAGTGGCGC ATCGACTGCA AGTGGAGTTC TCCAGAGAGC CGGAGAATGC GGATCTGCAG
    TTCTTGCGTC CTCTGTCAGT GAGTCTCCAG CAGGGGAACC GCAGCTCCCC AGACAGCGCC
    CCGCAGTGGT GGGTGGTGCA GGAGTGCTGG CCCGGACCTC CACCCAGCAG CTGCCGCAGT
    ATAGAGATGG TGGTGTTCAA TGATAAAGCC AGCCCGCCCA GCGTGGGCTT CCTGGCCGGA
    TACGGTATCG TGGGTCTGTA CATGTCCGTG GTGCTGGTCA TCGGGAAGTT CGTGCGGGAG
    TTCTTCACAG GGATCTCTCG GTCCATCATG TACGAGGAGC TGCCGTGTGT GGACCGCGTG
    CTCAAACTCT GCAACGACAT CTTCATAGTG CGCGAGGCGG GAGAGATGGA GATGGAGGAG
    CAGATGTTCG AGAAGCTCAT CTTCCTCTAC CGCTCTCCCG AAACCATGAT CAAGATGACC
    CACAACAAGA GCGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45828
    Clone ID Related Accession (Same CDS sequence) XM_009304391.2
    Accession Version XM_009304391.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product piezo-type mechanosensitive ion channel component 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGA TGGGCATCAG ATGAGGAAGA AGCGTTCACA GAAGAAGAGA
    AGCGTGCCGC TGAGCAGGAA GGAGAGAATG AAGAAATACA TGAATGACCG ACTCCTGGAG
    GCCAAAGCTG CGGTCATAGA GCTTGCTCTA CACATATACT TACCCATAAG GCAGTTCTTC
    TATGACATCA TCCACCCCGA GTACAGCCCT GTGTGTGACG TGTACGCGCT GATGTTCCTG
    ATAGACGTGG TCAACTTCAT CATCATTATA TTCGGATACT GGGCTTTTGG AAAGCACAGT
    GCGGCGGCTG ATATAACAGA GTCGCTGTCT GAAGACCAGG TTCCCGAGGC GTTCCTCGTC
    ATGCTCCTCA TCCAGTTCAG CACCATGATC GTGGACCGAG CGCTCTACCT GCGCAAAACC
    CTGCTGGGGA AGTGTGTGTT CCAGGTGGTG CTCGTCTTCG GGATACACTT CTGGATGTTC
    TTCATTTTGC CCGGTGTCAC TGAGAGGAGA TTCAACAGGA ACCGAGTTGC TCAGCTGTGG
    TACTTTGTAA AGTGCATCTA CTTTGGCCTC TCGGCGTATC AGATCAAGTG TGGATACCCA
    AACCGTGTGC TCGGAAACTT CCTCACCAAG AACCACAACT ACTTCAACCT CTTCCTCTTT
    CAGGGGTTCC GATTGGTTCC GTTCCTAACA GAGTTGCGGG CCGTAATGGA CTGGGTGTGG
    ACGGACACCA CTCTCAGCCT GTCCAACTGG ATCTGTGTGG AGGACATCTA CGCCAACATC
    TTCATCCTCA AGTGCTGGAG AGAAGCAGAA AAGAAATATC CCCATCGGCG TGGACAGCGG
    AAGAAGAAGG TGGTGAAGTA CACCATGGGC GGCTTCATCA TTTTTGCTCT GATCTGCATC
    GTCTGGTTCC CGCTGCTCTT CATGTCATTG GTTAAATCTG TCGCAGGAGT GACCAATCAG
    CCTTTGGACG TGTCCATCCA GCTGAGCATT CTGGGATACG AGCCGTTGTT CAGCATGAGT
    GCGCAGGAGC ACAATCTGAT CCCATACTCT CAATCAGCCT TCCAGAAGAT GACCAATCGC
    TTCGCCACGT ACCCGTCTGC CATGCAGTTC ATCGTGAACT ATATGGCAGA AGACATCGTG
    ACGGCGAAGA TCAAGAGCGA CGCCAGTCTG CTGTGGAGCG TCAGTCCTGC CAGCCGAGAC
    GCCATGATCT CTGAGCTCAG CAACTCCACA CACTTCTACA TCACACTGCG CTGGACTTTA
    CTACGAAATG CCTCTGTGTC GATGAATGCG GAGACGGCTG GTGAACACAC AGTGAAGTAT
    GAAGACCGTG CGCTGAGAGA CCAGCTGGTG GCGATGCTCA GAGGAACACA CACTCGCCCG
    CTGCTGCTGG AGAATCTGCT GCCCAAGTAT ATCAGAGGAG CGTCTGGACC CAATGCTAAA
    GTGGCGCATC GACTGCAAGT GGAGTTCTCC AGAGAGCCGG AGAATGCGGA TCTGCAGTTC
    TTGCGTCCTC TGTCAGTGAG TCTCCAGCAG GGGAACCGCA GCTCCCCAGA CAGCGCCCCG
    CAGTGGTGGG TGGTGCAGGA GTGCTGGCCC GGACCTCCAC CCAGCAGCTG CCGCAGTATA
    GAGATGGTGG TGTTCAATGA TAAAGCCAGC CCGCCCAGCG TGGGCTTCCT GGCCGGATAC
    GGTATCGTGG GTCTGTACAT GTCCGTGGTG CTGGTCATCG GGAAGTTCGT GCGGGAGTTC
    TTCACAGGGA TCTCTCGGTC CATCATGTAC GAGGAGCTGC CGTGTGTGGA CCGCGTGCTC
    AAACTCTGCA ACGACATCTT CATAGTGCGC GAGGCGGGAG AGATGGAGAT GGAGGAGCAG
    ATGTTCGAGA AGCTCATCTT CCTCTACCGC TCTCCCGAAA CCATGATCAA GATGACCCAC
    AACAAGAGCG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302666.1
    CDS156..7970
    Translation

    Target ORF information:

    RefSeq Version XM_009304391.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-11f4.7 (si:dkey-11f4.7), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304391.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGACTGGCG CTCGTCTCCC CTGCTGCTGT GTGCGCGCGC GTGCGATATC AGTGAGATTC 
    AGTGGATTAT CAGTGGTGTA TCTCCTGTTT CTGTTGTCCT TCCCTCTGAT ACCCACTCCA
    AACACGGCTA CTATCAAGGG GAAATCTGGC AGGTTTCTTC GCACTGTTTG CTCCACTGGC
    GTGATATTTC TGGCATTGCA ATGTGGGATG CAGATCACCT TCGCATATGT TCCCAAGGAT
    GAGACTGGAC CATGGGATGA TGTTTTCTTT CACCTTGGCA TTGTCAGGTT CAGTGAAGTG
    GACACACTAA ACATAATCCG CCTGTTAACT CCAGATGTGG TGGTGTCCGT GTCAACACTG
    TTCATCATCC GCTGGTGTAA AGCCAGAAAG CCCCATCAGA GTGAAGACGG CAGAGCGATG
    TACAGCACTG AGGGCTCGGA TGAAGAGATG TCGGACTCAG AATGTGAAGC GGAGAGCAGA
    GGATCTGAGG ACTCGTCTTT CGACACCTCC GCCGAGACCC GTATTTCTCC AGACGCATCT
    CCAGACGTCC TGCAGAAACT GATCCTGTTC GTGAGCGGTC TGAGACTCCT GGTGTCGTCT
    CTAATGAACA GTGCTGGAAG AGTCCTGGTC ACCTTTCTGC TGGGACTGGC GGGTATAACG
    TTCCCCTGTC TGACGTCGGC GCTGTATTTC AGTGTGTTTC TGGTGCTGGT GTGGTGCTGG
    GTGCTGGACT TCTCCATCTC TCTGCTGTTC TTCAGCTCTC TGTGTGTAAT GATGACCATC
    TTCAGTGGAG GACACGTGCT GATCCTCTAC CTGTACCAGC TGCCCCTCGC ACAGCAGATC
    ATTCCCCCTC ACGATCACTT CTCCAGGTTG TTTGGTTTGA CAGCCCTCAT CAAAACCAAC
    ATGTCAGAGC CGTACAGCCT GATCCTGCAT GAGGACAGCA GCTGGCCTGC AGTGGTGAAT
    CCGCTGGTCC TCCTGCTGCT GTATTTTACA GTCATGAGTC TCCTACACAA GTGGGCTCAC
    TCTGTTAATC AGGAAAATGC TGAAAACTAT GCTGAGGGCT CGAGCCTCTC CAAAATGCAC
    AATTTCCCCC TGGAAAGAAG AGTAGAGGCT ATGGTGTGGA GAAGGACATG GCAAGACCCG
    GAGGATTCTG GAGTCAATCT GCTTCTGGAG GACGACTATG CAAACTGCTT TGCTTTGTCT
    CCTTATACAC AGAAGATCTC TTATGAATAT ACTGTGGAGA AGGAGAGCTG TGACGGAGGG
    CATGAGATAT TAAATGATGT GGATCCTCCG CCTGATCAGT CTGGACCCAG CGCTGTGGTT
    CTGCTGGGAC AGTTCATCAT GAAACAGAGC TACATCATCG CTCTCATCAT CACAATGGTT
    TGGAGCATCT GCTATAACTC CTGGCTGACG TTAGTGATGC TGGTGTGGTC GTGTGTGATC
    TGGATGCTGA AGGACCGACG GCGGTTCGCC ATGCTCAGCG CTCCTTTCCT TGCCGTCTAC
    GGGACGCTGC TGCTCATAAT CTCGTTCATC AGTCAGCTTC ATCTGAAGCA TATTGCCGTT
    TTCCCTACTT TACCAAAGCT TGTCTTGATT GACTTTGATG CCTATGGTTA TCCTGTGCCC
    TGCGTGCACC TTGCTGCAAA GGTTTCTTAT AGTTTTAGTT TCTGGATTTT GCTTAGGCAA
    CGGCTGATAG AAAAACATGA AGAGGAGCTG CAGAACGAGG ATGAGCTAAA GGAAGTCAAA
    GTTCAAGCAC GTGACAGCAG TAATAGTTCT CCAATAATTG TAGTGTTGGG ATCATTAGTG
    AAAGGCATGT TGGTCAAGTA CTGGATCTTC TGCTGTTGCT TCATGTTCTT CTATGTCAGT
    TTCTCGGACA AAGTGGCGGT CTACAAAATC CTCTATATCT GCTTGTTCCT CTTCGGTGTG
    GTTCTGTATG AGGTTCATTA TGACGTATGG AGAAGTCAGC TGAAGTATTT CTGGGCCGTG
    GTGGTCGGAT ACTCCATGCT GGTGTTGATC CTGATCTACA TCTACCAGTT TAAGAGCGTC
    TGTAAGCTGT TTCAGCAGAT ATTAGGCCTG TCAGAGGAGA GCCTGCGAGA TCTTGGACTG
    GAGCAGTTTG ACACGGTTGA ACTCTTTGCA CGAATCTTAC GTCCAGCTTT CTTCCTCCTG
    GCCTGCATCC TTCAGCTGCA CTACTTCAAC TCGGACTTCC TCTGTCTGAC TGATCTGAAC
    ACAGTTACAA CTACAAACAG AGCAAAGAGA GAAGAAGACC TGAAAGAAAC GCTTCAGGGG
    GTTTTTGACA TAATGAAGAA AAGCTCTGAG AAATTGAAGA CATGGCTGAA GGACGGGCAG
    CTACGTGGAA AACAAGCCGA TCAAATGAAC ACCTCATCTT CCACCTTGGA CGAACATCAA
    GAGTTAGATT CAGAGGATGA TGTGCCGGTG GTGGTGCAGC AGTGGTTGTT GGTGTTGGAG
    CGGCTGCACG AGCTGATCCT GCGCTTCGTT CTGCTATTAT GGAGAGTTCA GGAGCTGTGC
    TGGAGGTTAC TGGAGCTCCA CAGCTTCAAA CTCGTCTCTA CCGGCATCAT CTGGGTGTGT
    GTGAAGGAGG TTTCACTGAT GAACTTTGTG TTTTTGGTGT TGTGGACGTT TGCTCTGCCC
    TATCCGAGAC TGCGGCCTGT GGCTTTCAAT GTGTCCTCTG TGTGGGCCTG TGTGATGGTC
    GTCTGTAAAA TGATGTACCA GCTGAAAAGC ATCAAACCCT CAGAGTATTC ATCAAACTGC
    ACTGCTGGTC TGTCCTCCTC CAATGAGAGT GGGACTCTTG ATGTTCCAGC TGATCTACTG
    GGGAATTCAG TTCTGTATTC GGCACCAGTG GACCCTTTCA TGTGGGTCGG CGGACTGAAG
    AAGTGTGAGG ATCGCATCCT GCCCTGCCTC TGGAATCACC TGTTGATTTT AGGTCTGCTT
    GTGGTGGACG TGACGGTCCA TCGACACCAG CTCCACCACC GCCTCCAACA CGGCCTAAAA
    ACAGCAAACA GCAGCATCAT ATTTCAGGCT GTCACACACC ATCACCTGGA CCACGGCATC
    CTGCCCTCCG TCAAATATTT TGTTAATTAT TTCTTCTACA AGTTTGGTTT AGAGGTGTGT
    TTTGTGGTAG CGGTGAACGT GATTGGCCAG CGGATGGATC TCTGTGCGCT TCTGCACTCC
    TTGGCTCTTA TGGTGGTTCT GGCTCGTCGG CGCAGGAAAG CCATTGGAGA AGTCTGGCCA
    AAATACTGCA GCTTCATCGC CAGCTTCATG GTGCTGCAGT ACCTGCTGTG CATCGGTGTA
    CCACCGGCCC TCTGCAGCGA TTATCCCTGG AGGAGTTCCT CTCCGGCTTT GAGTTCTAAC
    CTGATAAAGT GGCTGTATCT CCCAGACCAC GCCATGAGTC CCAACGCCAC CTTCATCCTC
    TATGACTGTC TGCTGCTGCT GGTGGCGTCG CTGCAGTGGA AGGTGTTCGA GGAGGAAAAT
    CAGGCCTGTG TGAGACTCCT CGCTGGAGAA AACGTGGAGA TCAGCCGGAA TCTGGATCCA
    CAGACACTCA ATCAGTACAC TCCTGTCAAC AACTTCCTTC ACTGCCGGTC ATATCTGGAC
    ATGGTGAAGG TGTTTGTGTT CAGTTATTTT TTCTGGCTGG TGTTGTGCCT CATCTTCATC
    ACGGGAACCA CCAGGATTAA CATGCTGTGT CTGGGTTATC TGGTGGCCTG CTTCTACTTC
    ATGCTGTTCG GAGGCACATT ACTGCTGCAG CCGGTGCAGT ATGTGCTGAG ACTGTGGGAC
    GCTCTGATAG CATACACCTG CGTGGTCATC AGCCTCAAAA ACTTGCTGTC GCTCGGCTCC
    TGTGCGTATC TGGATCCTCT ACAGAAGCAC GGCTGTTGGC TGATTCAGGC TTTTAGCATG
    TTCTGCACCA TCAAAGGCTA CAGTCTGCCG AAGCCTGACA GTCAGTGTGA GCTGCCGGAG
    GGTGAGGCGG GCATCATCTG GGATGCCATC TGTTTCACTG TGCTGCTGGC TCAGAGGAGA
    GTCTTCCTCA GCTACTACTT TCTCTATGTC GTGTCTGACC TGCAGACGTC TAAAATCCTC
    GCTTCAAGAG GTGCAGAATT GTTTGAAGCC AAAGTGAAGA AACAGGTGGC AGCGAGATTA
    GAGATGGAGA TCAAATCTGT GGAGATCCTG AAGAAACAAA TGGAGAAGAT CAAGTCAAAG
    CAGAATCCTC CTCCTCAGCA AGTGTCATCC TCTCCTGCTC ATCCTGAATC CTCACATCTG
    CCAAAGCGCA AAGATGGAGA CTGTGGATAC GATATGTTTG ACACTGACAG TGAGGAAGAG
    GAGGAGGAGG TGAAGGACAG GGCACAGGAG AGCATTTTAC CTATGAAGAA AACAGCCTTT
    CAGTTGGCGT ATGAGGCGTG GGTGGCGGGC CCGAAAGCAG CCCTCAACGC CTATAATCAG
    CAAGAACAAG AAGAAACCAG TAAAGAGAAA AGAAAGCTGC ACAGACAAGA GGGTATCGAC
    AAAGATGAGC CTGAAGAAGA GCTGCAGGAG GAGAGTCTTA AAGAAGAAGA AGGAGAAGAA
    GAAAGAGAGC ACATATTCCG GCGTATTCTT GACATGTTGA AGTTCCTCTG GGTTTTCCTT
    CTGCGTCTGA CGGAGGATGC CATATCAGGT CTGAATGCGC TCTGTAAAGA CAGCCTGAAC
    ATCTCTAAGG TGCTTCGCTT TGAGAAAACT CTACTGAGCA TACAGCAGAA CAAGGGCAGG
    GACGTCAATC ATGAGAGCAT TCATAAGTTC TACAAAACCT GGCTGTCCAG ACAAGAAACC
    GAGGACACGC TGGACACAGA AAAGGAGGAT GTTAAAGAAA TGACCTGCTC TCCTCCTGCA
    GTGAGCAGCT ACCCTCGGGG ATATGTCAAA CTCAACAGCC TACACTCATG GGGCAGCAGC
    CTGTCCAGCT GCCAGACTGA CGACACTCTC CTGGTGACCC GCCAGCCAAC TCTAGATGAC
    GTGGATGAGC TTGACTTCAC ACCGCTCGAC TCTGAGCAAC ACTGTGGAGA TGCCCAGTCA
    GAAACTCCTG ATATCAGTAA AGAGCGGAAG GATCCTGATA AAGGATGTGA AATGCTGACA
    GACGGATGGA CACATGAAGA AAACACCGCC GACATCGTGC TGACAGAGTT TCCCAAACAA
    ATGAATCTGC ACCAGGTGGA AAAAGAAGAG ACCCCAGAGC CCAGAACACT CAATCAGGAG
    ACCAGAGCAC TTACTGCCAG CGAACTGCTG CTTAACAACA TTTTTGATAT TCCAGAGATC
    GAGGAGTCAG ACAGATTCTA CTCGTCGCTG CCCCGTGGCC TGCGTCTCGT CTTTGCTCTG
    TATAATCTGA TGGAGTCTCA GTCGGAGATG TTCTGCTACT TTGTGATGAT CTTCAATCAT
    GTGGTGTCTG CTTCTCTGCT CACGCTCTTC CTGCCGATCC TCATCTTCCT GTGGGCCATG
    ATGTCGGTGC CACGACCAAC CAAGCGCTTC TGGATGACCG CTATCATCTA CACTCAGCTC
    ACAGTGGTGG TGAAGTACTT TTTCCAGTTC GGCTTCTTCC CATGGACCAC ATCAGCGTAC
    CTGGGCATCA ATGCTGAGCG AGCGTTCGCC GTACCCAACA TAGTAGGAGT GGAGAAGAAA
    GACGGATACG TCCTGGTAGA CCTCATTCAG TTAATCGCTC TGTTCTTCCA TCGCTCCATC
    CTCAAGTGTC ATGGATTGTG GGATAACAAG GAGGTGGAGA TGCCAGACTT CTTTAAGAAG
    CTAAAGAGGA AAACGGAGAA AAACAAGATG ACTGGAGGAT CGAGACAACG AGAAGAAAAC
    ACCAGCCGAC GAATACCCTT CCTTCCTCTT CAGGTCTCTT TCCTTCGGAG ACAGAGAGCA
    GAGAGTCCTG AGAGAGCGGA TGGGCATCAG ATGAGGAAGA AGCGTTCACA GAAGAAGAGA
    AGCGTGCCGC TGAGCAGGAA GGAGAGAATG AAGAAATACA TGAATGACCG ACTCCTGGAG
    GCCAAAGCTG CGGTCATAGA GCTTGCTCTA CACATATACT TACCCATAAG GCAGTTCTTC
    TATGACATCA TCCACCCCGA GTACAGCCCT GTGTGTGACG TGTACGCGCT GATGTTCCTG
    ATAGACGTGG TCAACTTCAT CATCATTATA TTCGGATACT GGGCTTTTGG AAAGCACAGT
    GCGGCGGCTG ATATAACAGA GTCGCTGTCT GAAGACCAGG TTCCCGAGGC GTTCCTCGTC
    ATGCTCCTCA TCCAGTTCAG CACCATGATC GTGGACCGAG CGCTCTACCT GCGCAAAACC
    CTGCTGGGGA AGTGTGTGTT CCAGGTGGTG CTCGTCTTCG GGATACACTT CTGGATGTTC
    TTCATTTTGC CCGGTGTCAC TGAGAGGAGA TTCAACAGGA ACCGAGTTGC TCAGCTGTGG
    TACTTTGTAA AGTGCATCTA CTTTGGCCTC TCGGCGTATC AGATCAAGTG TGGATACCCA
    AACCGTGTGC TCGGAAACTT CCTCACCAAG AACCACAACT ACTTCAACCT CTTCCTCTTT
    CAGGGGTTCC GATTGGTTCC GTTCCTAACA GAGTTGCGGG CCGTAATGGA CTGGGTGTGG
    ACGGACACCA CTCTCAGCCT GTCCAACTGG ATCTGTGTGG AGGACATCTA CGCCAACATC
    TTCATCCTCA AGTGCTGGAG AGAAGCAGAA AAGAAATATC CCCATCGGCG TGGACAGCGG
    AAGAAGAAGG TGGTGAAGTA CACCATGGGC GGCTTCATCA TTTTTGCTCT GATCTGCATC
    GTCTGGTTCC CGCTGCTCTT CATGTCATTG GTTAAATCTG TCGCAGGAGT GACCAATCAG
    CCTTTGGACG TGTCCATCCA GCTGAGCATT CTGGGATACG AGCCGTTGTT CAGCATGAGT
    GCGCAGGAGC ACAATCTGAT CCCATACTCT CAATCAGCCT TCCAGAAGAT GACCAATCGC
    TTCGCCACGT ACCCGTCTGC CATGCAGTTC ATCGTGAACT ATATGGCAGA AGACATCGTG
    ACGGCGAAGA TCAAGAGCGA CGCCAGTCTG CTGTGGAGCG TCAGTCCTGC CAGCCGAGAC
    GCCATGATCT CTGAGCTCAG CAACTCCACA CACTTCTACA TCACACTGCG CTGGACTTTA
    CTACGAAATG CCTCTGTGTC GATGAATGCG GAGACGGCTG GTGAACACAC AGTGAAGTAT
    GAAGACCGTG CGCTGAGAGA CCAGCTGGTG GCGATGCTCA GAGGAACACA CACTCGCCCG
    CTGCTGCTGG AGAATCTGCT GCCCAAGTAT ATCAGAGGAG CGTCTGGACC CAATGCTAAA
    GTGGCGCATC GACTGCAAGT GGAGTTCTCC AGAGAGCCGG AGAATGCGGA TCTGCAGTTC
    TTGCGTCCTC TGTCAGTGAG TCTCCAGCAG GGGAACCGCA GCTCCCCAGA CAGCGCCCCG
    CAGTGGTGGG TGGTGCAGGA GTGCTGGCCC GGACCTCCAC CCAGCAGCTG CCGCAGTATA
    GAGATGGTGG TGTTCAATGA TAAAGCCAGC CCGCCCAGCG TGGGCTTCCT GGCCGGATAC
    GGTATCGTGG GTCTGTACAT GTCCGTGGTG CTGGTCATCG GGAAGTTCGT GCGGGAGTTC
    TTCACAGGGA TCTCTCGGTC CATCATGTAC GAGGAGCTGC CGTGTGTGGA CCGCGTGCTC
    AAACTCTGCA ACGACATCTT CATAGTGCGC GAGGCGGGAG AGATGGAGAT GGAGGAGCAG
    ATGTTCGAGA AGCTCATCTT CCTCTACCGC TCTCCCGAAA CCATGATCAA GATGACCCAC
    AACAAGAGCG ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.