man2c1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	man2c1
Entrez Gene ID	569595
Full Name	mannosidase, alpha, class 2C, member 1
General protein information	Preferred Names mannosidase, alpha, class 2C, member 1 Names alpha-mannosidase 2C1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298093.2	XP_009296368.1	alpha-mannosidase 2C1 isoform X1
XM_017354269.1	XP_017209758.1	alpha-mannosidase 2C1 isoform X3
XM_692992.6	XP_698084.4	alpha-mannosidase 2C1 isoform X2
XM_009298093.3	XP_009296368.1	alpha-mannosidase 2C1 isoform X1
XM_692992.7	XP_698084.4	alpha-mannosidase 2C1 isoform X2
XM_017354269.2	XP_017209758.1	alpha-mannosidase 2C1 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Man2c1	NP_640349.1
Saccharomyces cerevisiae (baker's yeast)	AMS1	NP_011359.1
Magnaporthe oryzae (rice blast fungus)	MGG_04464	XP_003710997.1
	MAN2C1	XP_001102709.2
Eremothecium gossypii	AGOS_AGR385C	NP_987051.2
Schizosaccharomyces pombe (fission yeast)	ams1	NP_593979.1
Neurospora crassa	NCU07404	XP_958488.1
Xenopus tropicalis (tropical clawed frog)	man2c1	NP_001090678.1
Homo sapiens (human)	MAN2C1	NP_006706.2
Mus musculus (house mouse)	Man2c1	NP_082912.1
Danio rerio (zebrafish)	LOC569595	XP_698084.4
Pan troglodytes (chimpanzee)	MAN2C1	XP_003314839.1
Canis lupus familiaris (dog)	MAN2C1	XP_005638775.1

Gene Ontology

The following man2c1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man2c1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa57968	XM_017354269.2 Latest version!	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa29548	XM_692992.7 Latest version!	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45811	XM_009298093.3 Latest version!	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa45811	XM_009298093.2	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa29548	XM_692992.6	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa57968	XM_017354269.1	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

man2c1 ( XM_017354269.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209758.1 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57968
Clone ID Related Accession (Same CDS sequence)	XM_017354269.1 , XM_017354269.2
Accession Version	XM_017354269.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2C1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGC AGAATCAACA ACCAGTTCAG GGATTGACCA ATGAAGGTGA GAAGACAAGT 
TACATTCTGA CTGAGTGCTT GAAGGACAGT GAATCTCATG GGTTAGTGGA AATATGCAGT 
ATAGACAAAA TAATTACTCT ATATGTGGAA CTTGCATGTA ATGGACTTTT TGGAGCTGGA 
CAAGGTTCCA TGATTGCCGC CCCTGACCCA AACAGAAAGT TCACTCTACA GAAGGCAGAG 
CTTGTTGTAT TCAATCGAGA TGTCATGGAA CTTCTAACTG ATTTTGAGAT GCTTCTGGAC 
ATGGTCAAGG TACTAGGAGA AGGAGAGCAA CGTGGGTTTC AGGCTTTGTT TACAGCCAAT 
GAAATGGTGA ATCTGTGTGA TCCAGCCAAT CCTGGTTCAT TTGCTGCTGC TCGAAGTCTA 
GCTCAGAAGT TTTTTGCCCA GAAAAATGGG GAAAGCCAAC ATGTGGTACA TGCTATGGGA 
CACTGCCATA TTGATTCAGC TTGGTTGTGG CCATATGAAG AGACCATCAG GAAGTGTGCT 
CGCAGCTGGG TCACTGTTAT CAGACTGATG GAAAAGAACC CAGAGTTTGT CTTCACATGC 
TCCCAAGCAC AGCAGTTTCA GTGGGTGAAG AGCTGGTACC CGGGCCTGTT CTCAGAGATC 
AAGCACTTTA TACAGAAAGG CCAGTTTATT CCAGTTGGAG GCACTTGGGT AGAGATGGAT 
GGTAACTTGC CATCTGGTGA GTCCATGGTC AGACAGTTTC TACATGGCCA GAACTTCTTC 
AAGGACGAAT TTGGAAATTA CTGTAAAGAG TTTTGGCTAC CAGATACATT TGGTTACTCT 
GCACAGCTGC CTCAGATCAT GAAAGGGTGT GGGATCTCTC GTTTTATTAC TCAGAAGCTG 
AGCTGGAACC TTATTAACAC ATTTCCACAC AACACATTCT TTTGGGAGGG TATTGACGGG 
ACAGCAGTTC TCACTCATTT TCCTCCAGGT AACTCTTATG AGATGAAGGG TAAAGTGGAA 
GACTTGATTA ACACGGTGAA AAACAACAAA GACAAGGGAC GAGCAAATCA TAGTGCATTA 
TTGTTTGGTT TTGGAGACGG TGGTGGTGGA CCAACACAGC TAATGTTAGA CCGACTACAC 
CGTGTCAAGG ATACAGATTG TCTGCCTAAG GTTCAGATGT CAACTCCTGA TGTCCTGTTT 
TCTCACCTGG AATCAGACTC ATCGCTCCTT TGTAGATGGG TTGGTGAGCT CTTCCTTGAG 
CTACACAATG GCACTTACAC TACACAAGCA AAGATCAAGA TGAAAAATCG ACAATGTGAA 
ACACTGCTTC ATGATGTTGA GGTGGCAAGC TGTTTGGCCT TGTCTAAAAA GGATAGTTTC 
AAATACCCAG CATGCAGACT TCAGGAACTG TGGAGACTGC TTCTACTCAA CCAGTTCCAT 
GACGTGATTC CTGGCAGCTG TATTGGGATG GTTGTCGAGG ATGCATTACA GTACTATGAC 
GAAATACACA CCACGGCATC CAGACTTCTT CTTGATGCTT GCAAATCACT GCCGTCCTCT 
CCTGACAATG GTATTGCTGT GCTGAACACT TTATCTTGGG AGAGAACTGA AGTCATATCA 
CTACCAATGA AGGACACAGA AACAAGATTA GCCTTGGTGA GAGTCCCTAG CGTGGGTGTA 
GCTCATGTCA AGGAGACAGC AGAGTCTGCT GAGTCTGTTA TTGCCACAAT GATGGTCAAT 
GGAACCATCA TTATGGAAAA CGGTCTTTTG AAGGCAACTA TAGACCATCT AGGCCGTTTG 
ATGTCACTGC TTTTAATCCA AACAAACAGG GAAACCATTT CCGAGGGTTG CTTTGGGAAT 
CAGTTTGCAT TGTTTGATGA TGTACCTTTG TATTGGGATG CTTGGGATGT GATGGACTAC 
CATCTTCAAA CAAGGAAGCC TGTAATTGAG GTCATTGAAC CTGCCAAGGT GCTAAAATCA 
GGAGGAATTC AGGCCAGTGT CTCTTTCTCA CTAAGGATTA ACGGAAAAAG CACTATTCAA 
CAGGAGATTG TTCTAGATGC CTGCTGTCCC TATATCAAAT TCAAAACACA TGTGGTCTGG 
GAAGAGGCGC ATAAGTTTCT AAAGGTAGAG TTCCCTGTGC AGGTGCGCAG CCCAGAGGCT 
ACCTACGAGA TACAGTTTGG ACACTTGCAG AGGCCTACTC ATAGAAATAC CTCCTGGGAC 
TGGGCTCGTT TTGAGGTGTG GGGACACAAG TGGGCTGATC TGTCCGAGCA TGGTTTTGGC 
GTGGCTCTTC TTAACGACTC CAAATATGGC TACTCTGTCC ATCAAAACAT CATGACCTTG 
TCCCTTTTGC GGGCTCCAAA AGCTCCAGAT CTCAATGCTG ATATGGGGGA TCATATTTTT 
AGCTATGCGT TGATGCCACA TACAGGCACC TTTCAGGAAT CTGCAGTTAT CCAGTATGCC 
TACAACTTTA ACTACCCGCT TCGTGTCCAT CATGGTGTCA TTCCTAAACC CTGGAGTGCA 
TTCTCCGTAA GCTCACCTGC TATCATCCTG GAAACCATAA AAAAGGCGGA AAAAAGAGAG 
AATGCCCTTC TTATTCGCCT CTATGAGTCT CACGGAAGCC GTGTGACTGT GACATTGTCA 
ACTTCCTTTC TTTTACAAGA AGTCTGGCAT TGTGATCTCT TGGAGCGTCC AGATTTCAGA 
ATTCCTGTGT CAGAGACAAC CTCTGGAATA TCCTTAACAT TCAAACCATT TCAAATCAGA 
TCTCTCCTCT TGATTATACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209758.1
CDS	244..3027
Translation	MVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGLVEICSIDKIITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_017354269.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354269.1

ORF Insert Sequence:

ATGGTATGGC AGAATCAACA ACCAGTTCAG GGATTGACCA ATGAAGGTGA GAAGACAAGT 
TACATTCTGA CTGAGTGCTT GAAGGACAGT GAATCTCATG GGTTAGTGGA AATATGCAGT 
ATAGACAAAA TAATTACTCT ATATGTGGAA CTTGCATGTA ATGGACTTTT TGGAGCTGGA 
CAAGGTTCCA TGATTGCCGC CCCTGACCCA AACAGAAAGT TCACTCTACA GAAGGCAGAG 
CTTGTTGTAT TCAATCGAGA TGTCATGGAA CTTCTAACTG ATTTTGAGAT GCTTCTGGAC 
ATGGTCAAGG TACTAGGAGA AGGAGAGCAA CGTGGGTTTC AGGCTTTGTT TACAGCCAAT 
GAAATGGTGA ATCTGTGTGA TCCAGCCAAT CCTGGTTCAT TTGCTGCTGC TCGAAGTCTA 
GCTCAGAAGT TTTTTGCCCA GAAAAATGGG GAAAGCCAAC ATGTGGTACA TGCTATGGGA 
CACTGCCATA TTGATTCAGC TTGGTTGTGG CCATATGAAG AGACCATCAG GAAGTGTGCT 
CGCAGCTGGG TCACTGTTAT CAGACTGATG GAAAAGAACC CAGAGTTTGT CTTCACATGC 
TCCCAAGCAC AGCAGTTTCA GTGGGTGAAG AGCTGGTACC CGGGCCTGTT CTCAGAGATC 
AAGCACTTTA TACAGAAAGG CCAGTTTATT CCAGTTGGAG GCACTTGGGT AGAGATGGAT 
GGTAACTTGC CATCTGGTGA GTCCATGGTC AGACAGTTTC TACATGGCCA GAACTTCTTC 
AAGGACGAAT TTGGAAATTA CTGTAAAGAG TTTTGGCTAC CAGATACATT TGGTTACTCT 
GCACAGCTGC CTCAGATCAT GAAAGGGTGT GGGATCTCTC GTTTTATTAC TCAGAAGCTG 
AGCTGGAACC TTATTAACAC ATTTCCACAC AACACATTCT TTTGGGAGGG TATTGACGGG 
ACAGCAGTTC TCACTCATTT TCCTCCAGGT AACTCTTATG AGATGAAGGG TAAAGTGGAA 
GACTTGATTA ACACGGTGAA AAACAACAAA GACAAGGGAC GAGCAAATCA TAGTGCATTA 
TTGTTTGGTT TTGGAGACGG TGGTGGTGGA CCAACACAGC TAATGTTAGA CCGACTACAC 
CGTGTCAAGG ATACAGATTG TCTGCCTAAG GTTCAGATGT CAACTCCTGA TGTCCTGTTT 
TCTCACCTGG AATCAGACTC ATCGCTCCTT TGTAGATGGG TTGGTGAGCT CTTCCTTGAG 
CTACACAATG GCACTTACAC TACACAAGCA AAGATCAAGA TGAAAAATCG ACAATGTGAA 
ACACTGCTTC ATGATGTTGA GGTGGCAAGC TGTTTGGCCT TGTCTAAAAA GGATAGTTTC 
AAATACCCAG CATGCAGACT TCAGGAACTG TGGAGACTGC TTCTACTCAA CCAGTTCCAT 
GACGTGATTC CTGGCAGCTG TATTGGGATG GTTGTCGAGG ATGCATTACA GTACTATGAC 
GAAATACACA CCACGGCATC CAGACTTCTT CTTGATGCTT GCAAATCACT GCCGTCCTCT 
CCTGACAATG GTATTGCTGT GCTGAACACT TTATCTTGGG AGAGAACTGA AGTCATATCA 
CTACCAATGA AGGACACAGA AACAAGATTA GCCTTGGTGA GAGTCCCTAG CGTGGGTGTA 
GCTCATGTCA AGGAGACAGC AGAGTCTGCT GAGTCTGTTA TTGCCACAAT GATGGTCAAT 
GGAACCATCA TTATGGAAAA CGGTCTTTTG AAGGCAACTA TAGACCATCT AGGCCGTTTG 
ATGTCACTGC TTTTAATCCA AACAAACAGG GAAACCATTT CCGAGGGTTG CTTTGGGAAT 
CAGTTTGCAT TGTTTGATGA TGTACCTTTG TATTGGGATG CTTGGGATGT GATGGACTAC 
CATCTTCAAA CAAGGAAGCC TGTAATTGAG GTCATTGAAC CTGCCAAGGT GCTAAAATCA 
GGAGGAATTC AGGCCAGTGT CTCTTTCTCA CTAAGGATTA ACGGAAAAAG CACTATTCAA 
CAGGAGATTG TTCTAGATGC CTGCTGTCCC TATATCAAAT TCAAAACACA TGTGGTCTGG 
GAAGAGGCGC ATAAGTTTCT AAAGGTAGAG TTCCCTGTGC AGGTGCGCAG CCCAGAGGCT 
ACCTACGAGA TACAGTTTGG ACACTTGCAG AGGCCTACTC ATAGAAATAC CTCCTGGGAC 
TGGGCTCGTT TTGAGGTGTG GGGACACAAG TGGGCTGATC TGTCCGAGCA TGGTTTTGGC 
GTGGCTCTTC TTAACGACTC CAAATATGGC TACTCTGTCC ATCAAAACAT CATGACCTTG 
TCCCTTTTGC GGGCTCCAAA AGCTCCAGAT CTCAATGCTG ATATGGGGGA TCATATTTTT 
AGCTATGCGT TGATGCCACA TACAGGCACC TTTCAGGAAT CTGCAGTTAT CCAGTATGCC 
TACAACTTTA ACTACCCGCT TCGTGTCCAT CATGGTGTCA TTCCTAAACC CTGGAGTGCA 
TTCTCCGTAA GCTCACCTGC TATCATCCTG GAAACCATAA AAAAGGCGGA AAAAAGAGAG 
AATGCCCTTC TTATTCGCCT CTATGAGTCT CACGGAAGCC GTGTGACTGT GACATTGTCA 
ACTTCCTTTC TTTTACAAGA AGTCTGGCAT TGTGATCTCT TGGAGCGTCC AGATTTCAGA 
ATTCCTGTGT CAGAGACAAC CTCTGGAATA TCCTTAACAT TCAAACCATT TCAAATCAGA 
TCTCTCCTCT TGATTATACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2c1 ( XM_017354269.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209758.1 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57968
Clone ID Related Accession (Same CDS sequence)	XM_017354269.1 , XM_017354269.2
Accession Version	XM_017354269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2C1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATGGC AGAATCAACA ACCAGTTCAG GGATTGACCA ATGAAGGTGA GAAGACAAGT 
TACATTCTGA CTGAGTGCTT GAAGGACAGT GAATCTCATG GGTTAGTGGA AATATGCAGT 
ATAGACAAAA TAATTACTCT ATATGTGGAA CTTGCATGTA ATGGACTTTT TGGAGCTGGA 
CAAGGTTCCA TGATTGCCGC CCCTGACCCA AACAGAAAGT TCACTCTACA GAAGGCAGAG 
CTTGTTGTAT TCAATCGAGA TGTCATGGAA CTTCTAACTG ATTTTGAGAT GCTTCTGGAC 
ATGGTCAAGG TACTAGGAGA AGGAGAGCAA CGTGGGTTTC AGGCTTTGTT TACAGCCAAT 
GAAATGGTGA ATCTGTGTGA TCCAGCCAAT CCTGGTTCAT TTGCTGCTGC TCGAAGTCTA 
GCTCAGAAGT TTTTTGCCCA GAAAAATGGG GAAAGCCAAC ATGTGGTACA TGCTATGGGA 
CACTGCCATA TTGATTCAGC TTGGTTGTGG CCATATGAAG AGACCATCAG GAAGTGTGCT 
CGCAGCTGGG TCACTGTTAT CAGACTGATG GAAAAGAACC CAGAGTTTGT CTTCACATGC 
TCCCAAGCAC AGCAGTTTCA GTGGGTGAAG AGCTGGTACC CGGGCCTGTT CTCAGAGATC 
AAGCACTTTA TACAGAAAGG CCAGTTTATT CCAGTTGGAG GCACTTGGGT AGAGATGGAT 
GGTAACTTGC CATCTGGTGA GTCCATGGTC AGACAGTTTC TACATGGCCA GAACTTCTTC 
AAGGACGAAT TTGGAAATTA CTGTAAAGAG TTTTGGCTAC CAGATACATT TGGTTACTCT 
GCACAGCTGC CTCAGATCAT GAAAGGGTGT GGGATCTCTC GTTTTATTAC TCAGAAGCTG 
AGCTGGAACC TTATTAACAC ATTTCCACAC AACACATTCT TTTGGGAGGG TATTGACGGG 
ACAGCAGTTC TCACTCATTT TCCTCCAGGT AACTCTTATG AGATGAAGGG TAAAGTGGAA 
GACTTGATTA ACACGGTGAA AAACAACAAA GACAAGGGAC GAGCAAATCA TAGTGCATTA 
TTGTTTGGTT TTGGAGACGG TGGTGGTGGA CCAACACAGC TAATGTTAGA CCGACTACAC 
CGTGTCAAGG ATACAGATTG TCTGCCTAAG GTTCAGATGT CAACTCCTGA TGTCCTGTTT 
TCTCACCTGG AATCAGACTC ATCGCTCCTT TGTAGATGGG TTGGTGAGCT CTTCCTTGAG 
CTACACAATG GCACTTACAC TACACAAGCA AAGATCAAGA TGAAAAATCG ACAATGTGAA 
ACACTGCTTC ATGATGTTGA GGTGGCAAGC TGTTTGGCCT TGTCTAAAAA GGATAGTTTC 
AAATACCCAG CATGCAGACT TCAGGAACTG TGGAGACTGC TTCTACTCAA CCAGTTCCAT 
GACGTGATTC CTGGCAGCTG TATTGGGATG GTTGTCGAGG ATGCATTACA GTACTATGAC 
GAAATACACA CCACGGCATC CAGACTTCTT CTTGATGCTT GCAAATCACT GCCGTCCTCT 
CCTGACAATG GTATTGCTGT GCTGAACACT TTATCTTGGG AGAGAACTGA AGTCATATCA 
CTACCAATGA AGGACACAGA AACAAGATTA GCCTTGGTGA GAGTCCCTAG CGTGGGTGTA 
GCTCATGTCA AGGAGACAGC AGAGTCTGCT GAGTCTGTTA TTGCCACAAT GATGGTCAAT 
GGAACCATCA TTATGGAAAA CGGTCTTTTG AAGGCAACTA TAGACCATCT AGGCCGTTTG 
ATGTCACTGC TTTTAATCCA AACAAACAGG GAAACCATTT CCGAGGGTTG CTTTGGGAAT 
CAGTTTGCAT TGTTTGATGA TGTACCTTTG TATTGGGATG CTTGGGATGT GATGGACTAC 
CATCTTCAAA CAAGGAAGCC TGTAATTGAG GTCATTGAAC CTGCCAAGGT GCTAAAATCA 
GGAGGAATTC AGGCCAGTGT CTCTTTCTCA CTAAGGATTA ACGGAAAAAG CACTATTCAA 
CAGGAGATTG TTCTAGATGC CTGCTGTCCC TATATCAAAT TCAAAACACA TGTGGTCTGG 
GAAGAGGCGC ATAAGTTTCT AAAGGTAGAG TTCCCTGTGC AGGTGCGCAG CCCAGAGGCT 
ACCTACGAGA TACAGTTTGG ACACTTGCAG AGGCCTACTC ATAGAAATAC CTCCTGGGAC 
TGGGCTCGTT TTGAGGTGTG GGGACACAAG TGGGCTGATC TGTCCGAGCA TGGTTTTGGC 
GTGGCTCTTC TTAACGACTC CAAATATGGC TACTCTGTCC ATCAAAACAT CATGACCTTG 
TCCCTTTTGC GGGCTCCAAA AGCTCCAGAT CTCAATGCTG ATATGGGGGA TCATATTTTT 
AGCTATGCGT TGATGCCACA TACAGGCACC TTTCAGGAAT CTGCAGTTAT CCAGTATGCC 
TACAACTTTA ACTACCCGCT TCGTGTCCAT CATGGTGTCA TTCCTAAACC CTGGAGTGCA 
TTCTCCGTAA GCTCACCTGC TATCATCCTG GAAACCATAA AAAAGGCGGA AAAAAGAGAG 
AATGCCCTTC TTATTCGCCT CTATGAGTCT CACGGAAGCC GTGTGACTGT GACATTGTCA 
ACTTCCTTTC TTTTACAAGA AGTCTGGCAT TGTGATCTCT TGGAGCGTCC AGATTTCAGA 
ATTCCTGTGT CAGAGACAAC CTCTGGAATA TCCTTAACAT TCAAACCATT TCAAATCAGA 
TCTCTCCTCT TGATTATACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209758.1
CDS	308..3091
Translation	MVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGLVEICSIDKIITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_017354269.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354269.2

ORF Insert Sequence:

ATGGTATGGC AGAATCAACA ACCAGTTCAG GGATTGACCA ATGAAGGTGA GAAGACAAGT 
TACATTCTGA CTGAGTGCTT GAAGGACAGT GAATCTCATG GGTTAGTGGA AATATGCAGT 
ATAGACAAAA TAATTACTCT ATATGTGGAA CTTGCATGTA ATGGACTTTT TGGAGCTGGA 
CAAGGTTCCA TGATTGCCGC CCCTGACCCA AACAGAAAGT TCACTCTACA GAAGGCAGAG 
CTTGTTGTAT TCAATCGAGA TGTCATGGAA CTTCTAACTG ATTTTGAGAT GCTTCTGGAC 
ATGGTCAAGG TACTAGGAGA AGGAGAGCAA CGTGGGTTTC AGGCTTTGTT TACAGCCAAT 
GAAATGGTGA ATCTGTGTGA TCCAGCCAAT CCTGGTTCAT TTGCTGCTGC TCGAAGTCTA 
GCTCAGAAGT TTTTTGCCCA GAAAAATGGG GAAAGCCAAC ATGTGGTACA TGCTATGGGA 
CACTGCCATA TTGATTCAGC TTGGTTGTGG CCATATGAAG AGACCATCAG GAAGTGTGCT 
CGCAGCTGGG TCACTGTTAT CAGACTGATG GAAAAGAACC CAGAGTTTGT CTTCACATGC 
TCCCAAGCAC AGCAGTTTCA GTGGGTGAAG AGCTGGTACC CGGGCCTGTT CTCAGAGATC 
AAGCACTTTA TACAGAAAGG CCAGTTTATT CCAGTTGGAG GCACTTGGGT AGAGATGGAT 
GGTAACTTGC CATCTGGTGA GTCCATGGTC AGACAGTTTC TACATGGCCA GAACTTCTTC 
AAGGACGAAT TTGGAAATTA CTGTAAAGAG TTTTGGCTAC CAGATACATT TGGTTACTCT 
GCACAGCTGC CTCAGATCAT GAAAGGGTGT GGGATCTCTC GTTTTATTAC TCAGAAGCTG 
AGCTGGAACC TTATTAACAC ATTTCCACAC AACACATTCT TTTGGGAGGG TATTGACGGG 
ACAGCAGTTC TCACTCATTT TCCTCCAGGT AACTCTTATG AGATGAAGGG TAAAGTGGAA 
GACTTGATTA ACACGGTGAA AAACAACAAA GACAAGGGAC GAGCAAATCA TAGTGCATTA 
TTGTTTGGTT TTGGAGACGG TGGTGGTGGA CCAACACAGC TAATGTTAGA CCGACTACAC 
CGTGTCAAGG ATACAGATTG TCTGCCTAAG GTTCAGATGT CAACTCCTGA TGTCCTGTTT 
TCTCACCTGG AATCAGACTC ATCGCTCCTT TGTAGATGGG TTGGTGAGCT CTTCCTTGAG 
CTACACAATG GCACTTACAC TACACAAGCA AAGATCAAGA TGAAAAATCG ACAATGTGAA 
ACACTGCTTC ATGATGTTGA GGTGGCAAGC TGTTTGGCCT TGTCTAAAAA GGATAGTTTC 
AAATACCCAG CATGCAGACT TCAGGAACTG TGGAGACTGC TTCTACTCAA CCAGTTCCAT 
GACGTGATTC CTGGCAGCTG TATTGGGATG GTTGTCGAGG ATGCATTACA GTACTATGAC 
GAAATACACA CCACGGCATC CAGACTTCTT CTTGATGCTT GCAAATCACT GCCGTCCTCT 
CCTGACAATG GTATTGCTGT GCTGAACACT TTATCTTGGG AGAGAACTGA AGTCATATCA 
CTACCAATGA AGGACACAGA AACAAGATTA GCCTTGGTGA GAGTCCCTAG CGTGGGTGTA 
GCTCATGTCA AGGAGACAGC AGAGTCTGCT GAGTCTGTTA TTGCCACAAT GATGGTCAAT 
GGAACCATCA TTATGGAAAA CGGTCTTTTG AAGGCAACTA TAGACCATCT AGGCCGTTTG 
ATGTCACTGC TTTTAATCCA AACAAACAGG GAAACCATTT CCGAGGGTTG CTTTGGGAAT 
CAGTTTGCAT TGTTTGATGA TGTACCTTTG TATTGGGATG CTTGGGATGT GATGGACTAC 
CATCTTCAAA CAAGGAAGCC TGTAATTGAG GTCATTGAAC CTGCCAAGGT GCTAAAATCA 
GGAGGAATTC AGGCCAGTGT CTCTTTCTCA CTAAGGATTA ACGGAAAAAG CACTATTCAA 
CAGGAGATTG TTCTAGATGC CTGCTGTCCC TATATCAAAT TCAAAACACA TGTGGTCTGG 
GAAGAGGCGC ATAAGTTTCT AAAGGTAGAG TTCCCTGTGC AGGTGCGCAG CCCAGAGGCT 
ACCTACGAGA TACAGTTTGG ACACTTGCAG AGGCCTACTC ATAGAAATAC CTCCTGGGAC 
TGGGCTCGTT TTGAGGTGTG GGGACACAAG TGGGCTGATC TGTCCGAGCA TGGTTTTGGC 
GTGGCTCTTC TTAACGACTC CAAATATGGC TACTCTGTCC ATCAAAACAT CATGACCTTG 
TCCCTTTTGC GGGCTCCAAA AGCTCCAGAT CTCAATGCTG ATATGGGGGA TCATATTTTT 
AGCTATGCGT TGATGCCACA TACAGGCACC TTTCAGGAAT CTGCAGTTAT CCAGTATGCC 
TACAACTTTA ACTACCCGCT TCGTGTCCAT CATGGTGTCA TTCCTAAACC CTGGAGTGCA 
TTCTCCGTAA GCTCACCTGC TATCATCCTG GAAACCATAA AAAAGGCGGA AAAAAGAGAG 
AATGCCCTTC TTATTCGCCT CTATGAGTCT CACGGAAGCC GTGTGACTGT GACATTGTCA 
ACTTCCTTTC TTTTACAAGA AGTCTGGCAT TGTGATCTCT TGGAGCGTCC AGATTTCAGA 
ATTCCTGTGT CAGAGACAAC CTCTGGAATA TCCTTAACAT TCAAACCATT TCAAATCAGA 
TCTCTCCTCT TGATTATACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2c1 ( XM_692992.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_698084.4 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa29548
Clone ID Related Accession (Same CDS sequence)	XM_692992.6 , XM_692992.7
Accession Version	XM_692992.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2C1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_692992.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGAATTACTC TATATGTGGA ACTTGCATGT AATGGACTTT TTGGAGCTGG ACAAGGTTCC 
ATGATTGCCG CCCCTGACCC AAACAGAAAG TTCACTCTAC AGAAGGCAGA GCTTGTTGTA 
TTCAATCGAG ATGTCATGGA ACTTCTAACT GATTTTGAGA TGCTTCTGGA CATGGTCAAG 
GTACTAGGAG AAGGAGAGCA ACGTGGGTTT CAGGCTTTGT TTACAGCCAA TGAAATGGTG 
AATCTGTGTG ATCCAGCCAA TCCTGGTTCA TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG 
TTTTTTGCCC AGAAAAATGG GGAAAGCCAA CATGTGGTAC ATGCTATGGG ACACTGCCAT 
ATTGATTCAG CTTGGTTGTG GCCATATGAA GAGACCATCA GGAAGTGTGC TCGCAGCTGG 
GTCACTGTTA TCAGACTGAT GGAAAAGAAC CCAGAGTTTG TCTTCACATG CTCCCAAGCA 
CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC CCGGGCCTGT TCTCAGAGAT CAAGCACTTT 
ATACAGAAAG GCCAGTTTAT TCCAGTTGGA GGCACTTGGG TAGAGATGGA TGGTAACTTG 
CCATCTGGTG AGTCCATGGT CAGACAGTTT CTACATGGCC AGAACTTCTT CAAGGACGAA 
TTTGGAAATT ACTGTAAAGA GTTTTGGCTA CCAGATACAT TTGGTTACTC TGCACAGCTG 
CCTCAGATCA TGAAAGGGTG TGGGATCTCT CGTTTTATTA CTCAGAAGCT GAGCTGGAAC 
CTTATTAACA CATTTCCACA CAACACATTC TTTTGGGAGG GTATTGACGG GACAGCAGTT 
CTCACTCATT TTCCTCCAGG TAACTCTTAT GAGATGAAGG GTAAAGTGGA AGACTTGATT 
AACACGGTGA AAAACAACAA AGACAAGGGA CGAGCAAATC ATAGTGCATT ATTGTTTGGT 
TTTGGAGACG GTGGTGGTGG ACCAACACAG CTAATGTTAG ACCGACTACA CCGTGTCAAG 
GATACAGATT GTCTGCCTAA GGTTCAGATG TCAACTCCTG ATGTCCTGTT TTCTCACCTG 
GAATCAGACT CATCGCTCCT TTGTAGATGG GTTGGTGAGC TCTTCCTTGA GCTACACAAT 
GGCACTTACA CTACACAAGC AAAGATCAAG ATGAAAAATC GACAATGTGA AACACTGCTT 
CATGATGTTG AGGTGGCAAG CTGTTTGGCC TTGTCTAAAA AGGATAGTTT CAAATACCCA 
GCATGCAGAC TTCAGGAACT GTGGAGACTG CTTCTACTCA ACCAGTTCCA TGACGTGATT 
CCTGGCAGCT GTATTGGGAT GGTTGTCGAG GATGCATTAC AGTACTATGA CGAAATACAC 
ACCACGGCAT CCAGACTTCT TCTTGATGCT TGCAAATCAC TGCCGTCCTC TCCTGACAAT 
GGTATTGCTG TGCTGAACAC TTTATCTTGG GAGAGAACTG AAGTCATATC ACTACCAATG 
AAGGACACAG AAACAAGATT AGCCTTGGTG AGAGTCCCTA GCGTGGGTGT AGCTCATGTC 
AAGGAGACAG CAGAGTCTGC TGAGTCTGTT ATTGCCACAA TGATGGTCAA TGGAACCATC 
ATTATGGAAA ACGGTCTTTT GAAGGCAACT ATAGACCATC TAGGCCGTTT GATGTCACTG 
CTTTTAATCC AAACAAACAG GGAAACCATT TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA 
TTGTTTGATG ATGTACCTTT GTATTGGGAT GCTTGGGATG TGATGGACTA CCATCTTCAA 
ACAAGGAAGC CTGTAATTGA GGTCATTGAA CCTGCCAAGG TGCTAAAATC AGGAGGAATT 
CAGGCCAGTG TCTCTTTCTC ACTAAGGATT AACGGAAAAA GCACTATTCA ACAGGAGATT 
GTTCTAGATG CCTGCTGTCC CTATATCAAA TTCAAAACAC ATGTGGTCTG GGAAGAGGCG 
CATAAGTTTC TAAAGGTAGA GTTCCCTGTG CAGGTGCGCA GCCCAGAGGC TACCTACGAG 
ATACAGTTTG GACACTTGCA GAGGCCTACT CATAGAAATA CCTCCTGGGA CTGGGCTCGT 
TTTGAGGTGT GGGGACACAA GTGGGCTGAT CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT 
CTTAACGACT CCAAATATGG CTACTCTGTC CATCAAAACA TCATGACCTT GTCCCTTTTG 
CGGGCTCCAA AAGCTCCAGA TCTCAATGCT GATATGGGGG ATCATATTTT TAGCTATGCG 
TTGATGCCAC ATACAGGCAC CTTTCAGGAA TCTGCAGTTA TCCAGTATGC CTACAACTTT 
AACTACCCGC TTCGTGTCCA TCATGGTGTC ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA 
AGCTCACCTG CTATCATCCT GGAAACCATA AAAAAGGCGG AAAAAAGAGA GAATGCCCTT 
CTTATTCGCC TCTATGAGTC TCACGGAAGC CGTGTGACTG TGACATTGTC AACTTCCTTT 
CTTTTACAAG AAGTCTGGCA TTGTGATCTC TTGGAGCGTC CAGATTTCAG AATTCCTGTG 
TCAGAGACAA CCTCTGGAAT ATCCTTAACA TTCAAACCAT TTCAAATCAG ATCTCTCCTC 
TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698084.4
CDS	88..3162
Translation	MYHQPVLKNRRTLLERVEKFISDIYFTDCNLRGRLFGDIYPLESISVFLSEKRIPYSEAIQQNFQPCKVGDSFGPTWWTCWFKVNLKIPETWGGKEVHLRWESDGEGMVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_692992.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692992.6

ORF Insert Sequence:

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGAATTACTC TATATGTGGA ACTTGCATGT AATGGACTTT TTGGAGCTGG ACAAGGTTCC 
ATGATTGCCG CCCCTGACCC AAACAGAAAG TTCACTCTAC AGAAGGCAGA GCTTGTTGTA 
TTCAATCGAG ATGTCATGGA ACTTCTAACT GATTTTGAGA TGCTTCTGGA CATGGTCAAG 
GTACTAGGAG AAGGAGAGCA ACGTGGGTTT CAGGCTTTGT TTACAGCCAA TGAAATGGTG 
AATCTGTGTG ATCCAGCCAA TCCTGGTTCA TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG 
TTTTTTGCCC AGAAAAATGG GGAAAGCCAA CATGTGGTAC ATGCTATGGG ACACTGCCAT 
ATTGATTCAG CTTGGTTGTG GCCATATGAA GAGACCATCA GGAAGTGTGC TCGCAGCTGG 
GTCACTGTTA TCAGACTGAT GGAAAAGAAC CCAGAGTTTG TCTTCACATG CTCCCAAGCA 
CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC CCGGGCCTGT TCTCAGAGAT CAAGCACTTT 
ATACAGAAAG GCCAGTTTAT TCCAGTTGGA GGCACTTGGG TAGAGATGGA TGGTAACTTG 
CCATCTGGTG AGTCCATGGT CAGACAGTTT CTACATGGCC AGAACTTCTT CAAGGACGAA 
TTTGGAAATT ACTGTAAAGA GTTTTGGCTA CCAGATACAT TTGGTTACTC TGCACAGCTG 
CCTCAGATCA TGAAAGGGTG TGGGATCTCT CGTTTTATTA CTCAGAAGCT GAGCTGGAAC 
CTTATTAACA CATTTCCACA CAACACATTC TTTTGGGAGG GTATTGACGG GACAGCAGTT 
CTCACTCATT TTCCTCCAGG TAACTCTTAT GAGATGAAGG GTAAAGTGGA AGACTTGATT 
AACACGGTGA AAAACAACAA AGACAAGGGA CGAGCAAATC ATAGTGCATT ATTGTTTGGT 
TTTGGAGACG GTGGTGGTGG ACCAACACAG CTAATGTTAG ACCGACTACA CCGTGTCAAG 
GATACAGATT GTCTGCCTAA GGTTCAGATG TCAACTCCTG ATGTCCTGTT TTCTCACCTG 
GAATCAGACT CATCGCTCCT TTGTAGATGG GTTGGTGAGC TCTTCCTTGA GCTACACAAT 
GGCACTTACA CTACACAAGC AAAGATCAAG ATGAAAAATC GACAATGTGA AACACTGCTT 
CATGATGTTG AGGTGGCAAG CTGTTTGGCC TTGTCTAAAA AGGATAGTTT CAAATACCCA 
GCATGCAGAC TTCAGGAACT GTGGAGACTG CTTCTACTCA ACCAGTTCCA TGACGTGATT 
CCTGGCAGCT GTATTGGGAT GGTTGTCGAG GATGCATTAC AGTACTATGA CGAAATACAC 
ACCACGGCAT CCAGACTTCT TCTTGATGCT TGCAAATCAC TGCCGTCCTC TCCTGACAAT 
GGTATTGCTG TGCTGAACAC TTTATCTTGG GAGAGAACTG AAGTCATATC ACTACCAATG 
AAGGACACAG AAACAAGATT AGCCTTGGTG AGAGTCCCTA GCGTGGGTGT AGCTCATGTC 
AAGGAGACAG CAGAGTCTGC TGAGTCTGTT ATTGCCACAA TGATGGTCAA TGGAACCATC 
ATTATGGAAA ACGGTCTTTT GAAGGCAACT ATAGACCATC TAGGCCGTTT GATGTCACTG 
CTTTTAATCC AAACAAACAG GGAAACCATT TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA 
TTGTTTGATG ATGTACCTTT GTATTGGGAT GCTTGGGATG TGATGGACTA CCATCTTCAA 
ACAAGGAAGC CTGTAATTGA GGTCATTGAA CCTGCCAAGG TGCTAAAATC AGGAGGAATT 
CAGGCCAGTG TCTCTTTCTC ACTAAGGATT AACGGAAAAA GCACTATTCA ACAGGAGATT 
GTTCTAGATG CCTGCTGTCC CTATATCAAA TTCAAAACAC ATGTGGTCTG GGAAGAGGCG 
CATAAGTTTC TAAAGGTAGA GTTCCCTGTG CAGGTGCGCA GCCCAGAGGC TACCTACGAG 
ATACAGTTTG GACACTTGCA GAGGCCTACT CATAGAAATA CCTCCTGGGA CTGGGCTCGT 
TTTGAGGTGT GGGGACACAA GTGGGCTGAT CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT 
CTTAACGACT CCAAATATGG CTACTCTGTC CATCAAAACA TCATGACCTT GTCCCTTTTG 
CGGGCTCCAA AAGCTCCAGA TCTCAATGCT GATATGGGGG ATCATATTTT TAGCTATGCG 
TTGATGCCAC ATACAGGCAC CTTTCAGGAA TCTGCAGTTA TCCAGTATGC CTACAACTTT 
AACTACCCGC TTCGTGTCCA TCATGGTGTC ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA 
AGCTCACCTG CTATCATCCT GGAAACCATA AAAAAGGCGG AAAAAAGAGA GAATGCCCTT 
CTTATTCGCC TCTATGAGTC TCACGGAAGC CGTGTGACTG TGACATTGTC AACTTCCTTT 
CTTTTACAAG AAGTCTGGCA TTGTGATCTC TTGGAGCGTC CAGATTTCAG AATTCCTGTG 
TCAGAGACAA CCTCTGGAAT ATCCTTAACA TTCAAACCAT TTCAAATCAG ATCTCTCCTC 
TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2c1 ( XM_692992.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_698084.4 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa29548
Clone ID Related Accession (Same CDS sequence)	XM_692992.6 , XM_692992.7
Accession Version	XM_692992.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2C1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_692992.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGAATTACTC TATATGTGGA ACTTGCATGT AATGGACTTT TTGGAGCTGG ACAAGGTTCC 
ATGATTGCCG CCCCTGACCC AAACAGAAAG TTCACTCTAC AGAAGGCAGA GCTTGTTGTA 
TTCAATCGAG ATGTCATGGA ACTTCTAACT GATTTTGAGA TGCTTCTGGA CATGGTCAAG 
GTACTAGGAG AAGGAGAGCA ACGTGGGTTT CAGGCTTTGT TTACAGCCAA TGAAATGGTG 
AATCTGTGTG ATCCAGCCAA TCCTGGTTCA TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG 
TTTTTTGCCC AGAAAAATGG GGAAAGCCAA CATGTGGTAC ATGCTATGGG ACACTGCCAT 
ATTGATTCAG CTTGGTTGTG GCCATATGAA GAGACCATCA GGAAGTGTGC TCGCAGCTGG 
GTCACTGTTA TCAGACTGAT GGAAAAGAAC CCAGAGTTTG TCTTCACATG CTCCCAAGCA 
CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC CCGGGCCTGT TCTCAGAGAT CAAGCACTTT 
ATACAGAAAG GCCAGTTTAT TCCAGTTGGA GGCACTTGGG TAGAGATGGA TGGTAACTTG 
CCATCTGGTG AGTCCATGGT CAGACAGTTT CTACATGGCC AGAACTTCTT CAAGGACGAA 
TTTGGAAATT ACTGTAAAGA GTTTTGGCTA CCAGATACAT TTGGTTACTC TGCACAGCTG 
CCTCAGATCA TGAAAGGGTG TGGGATCTCT CGTTTTATTA CTCAGAAGCT GAGCTGGAAC 
CTTATTAACA CATTTCCACA CAACACATTC TTTTGGGAGG GTATTGACGG GACAGCAGTT 
CTCACTCATT TTCCTCCAGG TAACTCTTAT GAGATGAAGG GTAAAGTGGA AGACTTGATT 
AACACGGTGA AAAACAACAA AGACAAGGGA CGAGCAAATC ATAGTGCATT ATTGTTTGGT 
TTTGGAGACG GTGGTGGTGG ACCAACACAG CTAATGTTAG ACCGACTACA CCGTGTCAAG 
GATACAGATT GTCTGCCTAA GGTTCAGATG TCAACTCCTG ATGTCCTGTT TTCTCACCTG 
GAATCAGACT CATCGCTCCT TTGTAGATGG GTTGGTGAGC TCTTCCTTGA GCTACACAAT 
GGCACTTACA CTACACAAGC AAAGATCAAG ATGAAAAATC GACAATGTGA AACACTGCTT 
CATGATGTTG AGGTGGCAAG CTGTTTGGCC TTGTCTAAAA AGGATAGTTT CAAATACCCA 
GCATGCAGAC TTCAGGAACT GTGGAGACTG CTTCTACTCA ACCAGTTCCA TGACGTGATT 
CCTGGCAGCT GTATTGGGAT GGTTGTCGAG GATGCATTAC AGTACTATGA CGAAATACAC 
ACCACGGCAT CCAGACTTCT TCTTGATGCT TGCAAATCAC TGCCGTCCTC TCCTGACAAT 
GGTATTGCTG TGCTGAACAC TTTATCTTGG GAGAGAACTG AAGTCATATC ACTACCAATG 
AAGGACACAG AAACAAGATT AGCCTTGGTG AGAGTCCCTA GCGTGGGTGT AGCTCATGTC 
AAGGAGACAG CAGAGTCTGC TGAGTCTGTT ATTGCCACAA TGATGGTCAA TGGAACCATC 
ATTATGGAAA ACGGTCTTTT GAAGGCAACT ATAGACCATC TAGGCCGTTT GATGTCACTG 
CTTTTAATCC AAACAAACAG GGAAACCATT TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA 
TTGTTTGATG ATGTACCTTT GTATTGGGAT GCTTGGGATG TGATGGACTA CCATCTTCAA 
ACAAGGAAGC CTGTAATTGA GGTCATTGAA CCTGCCAAGG TGCTAAAATC AGGAGGAATT 
CAGGCCAGTG TCTCTTTCTC ACTAAGGATT AACGGAAAAA GCACTATTCA ACAGGAGATT 
GTTCTAGATG CCTGCTGTCC CTATATCAAA TTCAAAACAC ATGTGGTCTG GGAAGAGGCG 
CATAAGTTTC TAAAGGTAGA GTTCCCTGTG CAGGTGCGCA GCCCAGAGGC TACCTACGAG 
ATACAGTTTG GACACTTGCA GAGGCCTACT CATAGAAATA CCTCCTGGGA CTGGGCTCGT 
TTTGAGGTGT GGGGACACAA GTGGGCTGAT CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT 
CTTAACGACT CCAAATATGG CTACTCTGTC CATCAAAACA TCATGACCTT GTCCCTTTTG 
CGGGCTCCAA AAGCTCCAGA TCTCAATGCT GATATGGGGG ATCATATTTT TAGCTATGCG 
TTGATGCCAC ATACAGGCAC CTTTCAGGAA TCTGCAGTTA TCCAGTATGC CTACAACTTT 
AACTACCCGC TTCGTGTCCA TCATGGTGTC ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA 
AGCTCACCTG CTATCATCCT GGAAACCATA AAAAAGGCGG AAAAAAGAGA GAATGCCCTT 
CTTATTCGCC TCTATGAGTC TCACGGAAGC CGTGTGACTG TGACATTGTC AACTTCCTTT 
CTTTTACAAG AAGTCTGGCA TTGTGATCTC TTGGAGCGTC CAGATTTCAG AATTCCTGTG 
TCAGAGACAA CCTCTGGAAT ATCCTTAACA TTCAAACCAT TTCAAATCAG ATCTCTCCTC 
TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698084.4
CDS	92..3166
Translation	MYHQPVLKNRRTLLERVEKFISDIYFTDCNLRGRLFGDIYPLESISVFLSEKRIPYSEAIQQNFQPCKVGDSFGPTWWTCWFKVNLKIPETWGGKEVHLRWESDGEGMVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_692992.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692992.7

ORF Insert Sequence:

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGAATTACTC TATATGTGGA ACTTGCATGT AATGGACTTT TTGGAGCTGG ACAAGGTTCC 
ATGATTGCCG CCCCTGACCC AAACAGAAAG TTCACTCTAC AGAAGGCAGA GCTTGTTGTA 
TTCAATCGAG ATGTCATGGA ACTTCTAACT GATTTTGAGA TGCTTCTGGA CATGGTCAAG 
GTACTAGGAG AAGGAGAGCA ACGTGGGTTT CAGGCTTTGT TTACAGCCAA TGAAATGGTG 
AATCTGTGTG ATCCAGCCAA TCCTGGTTCA TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG 
TTTTTTGCCC AGAAAAATGG GGAAAGCCAA CATGTGGTAC ATGCTATGGG ACACTGCCAT 
ATTGATTCAG CTTGGTTGTG GCCATATGAA GAGACCATCA GGAAGTGTGC TCGCAGCTGG 
GTCACTGTTA TCAGACTGAT GGAAAAGAAC CCAGAGTTTG TCTTCACATG CTCCCAAGCA 
CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC CCGGGCCTGT TCTCAGAGAT CAAGCACTTT 
ATACAGAAAG GCCAGTTTAT TCCAGTTGGA GGCACTTGGG TAGAGATGGA TGGTAACTTG 
CCATCTGGTG AGTCCATGGT CAGACAGTTT CTACATGGCC AGAACTTCTT CAAGGACGAA 
TTTGGAAATT ACTGTAAAGA GTTTTGGCTA CCAGATACAT TTGGTTACTC TGCACAGCTG 
CCTCAGATCA TGAAAGGGTG TGGGATCTCT CGTTTTATTA CTCAGAAGCT GAGCTGGAAC 
CTTATTAACA CATTTCCACA CAACACATTC TTTTGGGAGG GTATTGACGG GACAGCAGTT 
CTCACTCATT TTCCTCCAGG TAACTCTTAT GAGATGAAGG GTAAAGTGGA AGACTTGATT 
AACACGGTGA AAAACAACAA AGACAAGGGA CGAGCAAATC ATAGTGCATT ATTGTTTGGT 
TTTGGAGACG GTGGTGGTGG ACCAACACAG CTAATGTTAG ACCGACTACA CCGTGTCAAG 
GATACAGATT GTCTGCCTAA GGTTCAGATG TCAACTCCTG ATGTCCTGTT TTCTCACCTG 
GAATCAGACT CATCGCTCCT TTGTAGATGG GTTGGTGAGC TCTTCCTTGA GCTACACAAT 
GGCACTTACA CTACACAAGC AAAGATCAAG ATGAAAAATC GACAATGTGA AACACTGCTT 
CATGATGTTG AGGTGGCAAG CTGTTTGGCC TTGTCTAAAA AGGATAGTTT CAAATACCCA 
GCATGCAGAC TTCAGGAACT GTGGAGACTG CTTCTACTCA ACCAGTTCCA TGACGTGATT 
CCTGGCAGCT GTATTGGGAT GGTTGTCGAG GATGCATTAC AGTACTATGA CGAAATACAC 
ACCACGGCAT CCAGACTTCT TCTTGATGCT TGCAAATCAC TGCCGTCCTC TCCTGACAAT 
GGTATTGCTG TGCTGAACAC TTTATCTTGG GAGAGAACTG AAGTCATATC ACTACCAATG 
AAGGACACAG AAACAAGATT AGCCTTGGTG AGAGTCCCTA GCGTGGGTGT AGCTCATGTC 
AAGGAGACAG CAGAGTCTGC TGAGTCTGTT ATTGCCACAA TGATGGTCAA TGGAACCATC 
ATTATGGAAA ACGGTCTTTT GAAGGCAACT ATAGACCATC TAGGCCGTTT GATGTCACTG 
CTTTTAATCC AAACAAACAG GGAAACCATT TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA 
TTGTTTGATG ATGTACCTTT GTATTGGGAT GCTTGGGATG TGATGGACTA CCATCTTCAA 
ACAAGGAAGC CTGTAATTGA GGTCATTGAA CCTGCCAAGG TGCTAAAATC AGGAGGAATT 
CAGGCCAGTG TCTCTTTCTC ACTAAGGATT AACGGAAAAA GCACTATTCA ACAGGAGATT 
GTTCTAGATG CCTGCTGTCC CTATATCAAA TTCAAAACAC ATGTGGTCTG GGAAGAGGCG 
CATAAGTTTC TAAAGGTAGA GTTCCCTGTG CAGGTGCGCA GCCCAGAGGC TACCTACGAG 
ATACAGTTTG GACACTTGCA GAGGCCTACT CATAGAAATA CCTCCTGGGA CTGGGCTCGT 
TTTGAGGTGT GGGGACACAA GTGGGCTGAT CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT 
CTTAACGACT CCAAATATGG CTACTCTGTC CATCAAAACA TCATGACCTT GTCCCTTTTG 
CGGGCTCCAA AAGCTCCAGA TCTCAATGCT GATATGGGGG ATCATATTTT TAGCTATGCG 
TTGATGCCAC ATACAGGCAC CTTTCAGGAA TCTGCAGTTA TCCAGTATGC CTACAACTTT 
AACTACCCGC TTCGTGTCCA TCATGGTGTC ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA 
AGCTCACCTG CTATCATCCT GGAAACCATA AAAAAGGCGG AAAAAAGAGA GAATGCCCTT 
CTTATTCGCC TCTATGAGTC TCACGGAAGC CGTGTGACTG TGACATTGTC AACTTCCTTT 
CTTTTACAAG AAGTCTGGCA TTGTGATCTC TTGGAGCGTC CAGATTTCAG AATTCCTGTG 
TCAGAGACAA CCTCTGGAAT ATCCTTAACA TTCAAACCAT TTCAAATCAG ATCTCTCCTC 
TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2c1 ( XM_009298093.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296368.1 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45811
Clone ID Related Accession (Same CDS sequence)	XM_009298093.2 , XM_009298093.3
Accession Version	XM_009298093.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-mannosidase 2C1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGGTTAGTGG AAATATGCAG TATAGACAAA ATAATTACTC TATATGTGGA ACTTGCATGT 
AATGGACTTT TTGGAGCTGG ACAAGGTTCC ATGATTGCCG CCCCTGACCC AAACAGAAAG 
TTCACTCTAC AGAAGGCAGA GCTTGTTGTA TTCAATCGAG ATGTCATGGA ACTTCTAACT 
GATTTTGAGA TGCTTCTGGA CATGGTCAAG GTACTAGGAG AAGGAGAGCA ACGTGGGTTT 
CAGGCTTTGT TTACAGCCAA TGAAATGGTG AATCTGTGTG ATCCAGCCAA TCCTGGTTCA 
TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG TTTTTTGCCC AGAAAAATGG GGAAAGCCAA 
CATGTGGTAC ATGCTATGGG ACACTGCCAT ATTGATTCAG CTTGGTTGTG GCCATATGAA 
GAGACCATCA GGAAGTGTGC TCGCAGCTGG GTCACTGTTA TCAGACTGAT GGAAAAGAAC 
CCAGAGTTTG TCTTCACATG CTCCCAAGCA CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC 
CCGGGCCTGT TCTCAGAGAT CAAGCACTTT ATACAGAAAG GCCAGTTTAT TCCAGTTGGA 
GGCACTTGGG TAGAGATGGA TGGTAACTTG CCATCTGGTG AGTCCATGGT CAGACAGTTT 
CTACATGGCC AGAACTTCTT CAAGGACGAA TTTGGAAATT ACTGTAAAGA GTTTTGGCTA 
CCAGATACAT TTGGTTACTC TGCACAGCTG CCTCAGATCA TGAAAGGGTG TGGGATCTCT 
CGTTTTATTA CTCAGAAGCT GAGCTGGAAC CTTATTAACA CATTTCCACA CAACACATTC 
TTTTGGGAGG GTATTGACGG GACAGCAGTT CTCACTCATT TTCCTCCAGG TAACTCTTAT 
GAGATGAAGG GTAAAGTGGA AGACTTGATT AACACGGTGA AAAACAACAA AGACAAGGGA 
CGAGCAAATC ATAGTGCATT ATTGTTTGGT TTTGGAGACG GTGGTGGTGG ACCAACACAG 
CTAATGTTAG ACCGACTACA CCGTGTCAAG GATACAGATT GTCTGCCTAA GGTTCAGATG 
TCAACTCCTG ATGTCCTGTT TTCTCACCTG GAATCAGACT CATCGCTCCT TTGTAGATGG 
GTTGGTGAGC TCTTCCTTGA GCTACACAAT GGCACTTACA CTACACAAGC AAAGATCAAG 
ATGAAAAATC GACAATGTGA AACACTGCTT CATGATGTTG AGGTGGCAAG CTGTTTGGCC 
TTGTCTAAAA AGGATAGTTT CAAATACCCA GCATGCAGAC TTCAGGAACT GTGGAGACTG 
CTTCTACTCA ACCAGTTCCA TGACGTGATT CCTGGCAGCT GTATTGGGAT GGTTGTCGAG 
GATGCATTAC AGTACTATGA CGAAATACAC ACCACGGCAT CCAGACTTCT TCTTGATGCT 
TGCAAATCAC TGCCGTCCTC TCCTGACAAT GGTATTGCTG TGCTGAACAC TTTATCTTGG 
GAGAGAACTG AAGTCATATC ACTACCAATG AAGGACACAG AAACAAGATT AGCCTTGGTG 
AGAGTCCCTA GCGTGGGTGT AGCTCATGTC AAGGAGACAG CAGAGTCTGC TGAGTCTGTT 
ATTGCCACAA TGATGGTCAA TGGAACCATC ATTATGGAAA ACGGTCTTTT GAAGGCAACT 
ATAGACCATC TAGGCCGTTT GATGTCACTG CTTTTAATCC AAACAAACAG GGAAACCATT 
TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA TTGTTTGATG ATGTACCTTT GTATTGGGAT 
GCTTGGGATG TGATGGACTA CCATCTTCAA ACAAGGAAGC CTGTAATTGA GGTCATTGAA 
CCTGCCAAGG TGCTAAAATC AGGAGGAATT CAGGCCAGTG TCTCTTTCTC ACTAAGGATT 
AACGGAAAAA GCACTATTCA ACAGGAGATT GTTCTAGATG CCTGCTGTCC CTATATCAAA 
TTCAAAACAC ATGTGGTCTG GGAAGAGGCG CATAAGTTTC TAAAGGTAGA GTTCCCTGTG 
CAGGTGCGCA GCCCAGAGGC TACCTACGAG ATACAGTTTG GACACTTGCA GAGGCCTACT 
CATAGAAATA CCTCCTGGGA CTGGGCTCGT TTTGAGGTGT GGGGACACAA GTGGGCTGAT 
CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT CTTAACGACT CCAAATATGG CTACTCTGTC 
CATCAAAACA TCATGACCTT GTCCCTTTTG CGGGCTCCAA AAGCTCCAGA TCTCAATGCT 
GATATGGGGG ATCATATTTT TAGCTATGCG TTGATGCCAC ATACAGGCAC CTTTCAGGAA 
TCTGCAGTTA TCCAGTATGC CTACAACTTT AACTACCCGC TTCGTGTCCA TCATGGTGTC 
ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA AGCTCACCTG CTATCATCCT GGAAACCATA 
AAAAAGGCGG AAAAAAGAGA GAATGCCCTT CTTATTCGCC TCTATGAGTC TCACGGAAGC 
CGTGTGACTG TGACATTGTC AACTTCCTTT CTTTTACAAG AAGTCTGGCA TTGTGATCTC 
TTGGAGCGTC CAGATTTCAG AATTCCTGTG TCAGAGACAA CCTCTGGAAT ATCCTTAACA 
TTCAAACCAT TTCAAATCAG ATCTCTCCTC TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296368.1
CDS	88..3192
Translation	MYHQPVLKNRRTLLERVEKFISDIYFTDCNLRGRLFGDIYPLESISVFLSEKRIPYSEAIQQNFQPCKVGDSFGPTWWTCWFKVNLKIPETWGGKEVHLRWESDGEGMVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGLVEICSIDKIITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_009298093.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298093.2

ORF Insert Sequence:

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGGTTAGTGG AAATATGCAG TATAGACAAA ATAATTACTC TATATGTGGA ACTTGCATGT 
AATGGACTTT TTGGAGCTGG ACAAGGTTCC ATGATTGCCG CCCCTGACCC AAACAGAAAG 
TTCACTCTAC AGAAGGCAGA GCTTGTTGTA TTCAATCGAG ATGTCATGGA ACTTCTAACT 
GATTTTGAGA TGCTTCTGGA CATGGTCAAG GTACTAGGAG AAGGAGAGCA ACGTGGGTTT 
CAGGCTTTGT TTACAGCCAA TGAAATGGTG AATCTGTGTG ATCCAGCCAA TCCTGGTTCA 
TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG TTTTTTGCCC AGAAAAATGG GGAAAGCCAA 
CATGTGGTAC ATGCTATGGG ACACTGCCAT ATTGATTCAG CTTGGTTGTG GCCATATGAA 
GAGACCATCA GGAAGTGTGC TCGCAGCTGG GTCACTGTTA TCAGACTGAT GGAAAAGAAC 
CCAGAGTTTG TCTTCACATG CTCCCAAGCA CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC 
CCGGGCCTGT TCTCAGAGAT CAAGCACTTT ATACAGAAAG GCCAGTTTAT TCCAGTTGGA 
GGCACTTGGG TAGAGATGGA TGGTAACTTG CCATCTGGTG AGTCCATGGT CAGACAGTTT 
CTACATGGCC AGAACTTCTT CAAGGACGAA TTTGGAAATT ACTGTAAAGA GTTTTGGCTA 
CCAGATACAT TTGGTTACTC TGCACAGCTG CCTCAGATCA TGAAAGGGTG TGGGATCTCT 
CGTTTTATTA CTCAGAAGCT GAGCTGGAAC CTTATTAACA CATTTCCACA CAACACATTC 
TTTTGGGAGG GTATTGACGG GACAGCAGTT CTCACTCATT TTCCTCCAGG TAACTCTTAT 
GAGATGAAGG GTAAAGTGGA AGACTTGATT AACACGGTGA AAAACAACAA AGACAAGGGA 
CGAGCAAATC ATAGTGCATT ATTGTTTGGT TTTGGAGACG GTGGTGGTGG ACCAACACAG 
CTAATGTTAG ACCGACTACA CCGTGTCAAG GATACAGATT GTCTGCCTAA GGTTCAGATG 
TCAACTCCTG ATGTCCTGTT TTCTCACCTG GAATCAGACT CATCGCTCCT TTGTAGATGG 
GTTGGTGAGC TCTTCCTTGA GCTACACAAT GGCACTTACA CTACACAAGC AAAGATCAAG 
ATGAAAAATC GACAATGTGA AACACTGCTT CATGATGTTG AGGTGGCAAG CTGTTTGGCC 
TTGTCTAAAA AGGATAGTTT CAAATACCCA GCATGCAGAC TTCAGGAACT GTGGAGACTG 
CTTCTACTCA ACCAGTTCCA TGACGTGATT CCTGGCAGCT GTATTGGGAT GGTTGTCGAG 
GATGCATTAC AGTACTATGA CGAAATACAC ACCACGGCAT CCAGACTTCT TCTTGATGCT 
TGCAAATCAC TGCCGTCCTC TCCTGACAAT GGTATTGCTG TGCTGAACAC TTTATCTTGG 
GAGAGAACTG AAGTCATATC ACTACCAATG AAGGACACAG AAACAAGATT AGCCTTGGTG 
AGAGTCCCTA GCGTGGGTGT AGCTCATGTC AAGGAGACAG CAGAGTCTGC TGAGTCTGTT 
ATTGCCACAA TGATGGTCAA TGGAACCATC ATTATGGAAA ACGGTCTTTT GAAGGCAACT 
ATAGACCATC TAGGCCGTTT GATGTCACTG CTTTTAATCC AAACAAACAG GGAAACCATT 
TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA TTGTTTGATG ATGTACCTTT GTATTGGGAT 
GCTTGGGATG TGATGGACTA CCATCTTCAA ACAAGGAAGC CTGTAATTGA GGTCATTGAA 
CCTGCCAAGG TGCTAAAATC AGGAGGAATT CAGGCCAGTG TCTCTTTCTC ACTAAGGATT 
AACGGAAAAA GCACTATTCA ACAGGAGATT GTTCTAGATG CCTGCTGTCC CTATATCAAA 
TTCAAAACAC ATGTGGTCTG GGAAGAGGCG CATAAGTTTC TAAAGGTAGA GTTCCCTGTG 
CAGGTGCGCA GCCCAGAGGC TACCTACGAG ATACAGTTTG GACACTTGCA GAGGCCTACT 
CATAGAAATA CCTCCTGGGA CTGGGCTCGT TTTGAGGTGT GGGGACACAA GTGGGCTGAT 
CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT CTTAACGACT CCAAATATGG CTACTCTGTC 
CATCAAAACA TCATGACCTT GTCCCTTTTG CGGGCTCCAA AAGCTCCAGA TCTCAATGCT 
GATATGGGGG ATCATATTTT TAGCTATGCG TTGATGCCAC ATACAGGCAC CTTTCAGGAA 
TCTGCAGTTA TCCAGTATGC CTACAACTTT AACTACCCGC TTCGTGTCCA TCATGGTGTC 
ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA AGCTCACCTG CTATCATCCT GGAAACCATA 
AAAAAGGCGG AAAAAAGAGA GAATGCCCTT CTTATTCGCC TCTATGAGTC TCACGGAAGC 
CGTGTGACTG TGACATTGTC AACTTCCTTT CTTTTACAAG AAGTCTGGCA TTGTGATCTC 
TTGGAGCGTC CAGATTTCAG AATTCCTGTG TCAGAGACAA CCTCTGGAAT ATCCTTAACA 
TTCAAACCAT TTCAAATCAG ATCTCTCCTC TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2c1 ( XM_009298093.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296368.1 Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45811
Clone ID Related Accession (Same CDS sequence)	XM_009298093.2 , XM_009298093.3
Accession Version	XM_009298093.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-mannosidase 2C1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGGTTAGTGG AAATATGCAG TATAGACAAA ATAATTACTC TATATGTGGA ACTTGCATGT 
AATGGACTTT TTGGAGCTGG ACAAGGTTCC ATGATTGCCG CCCCTGACCC AAACAGAAAG 
TTCACTCTAC AGAAGGCAGA GCTTGTTGTA TTCAATCGAG ATGTCATGGA ACTTCTAACT 
GATTTTGAGA TGCTTCTGGA CATGGTCAAG GTACTAGGAG AAGGAGAGCA ACGTGGGTTT 
CAGGCTTTGT TTACAGCCAA TGAAATGGTG AATCTGTGTG ATCCAGCCAA TCCTGGTTCA 
TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG TTTTTTGCCC AGAAAAATGG GGAAAGCCAA 
CATGTGGTAC ATGCTATGGG ACACTGCCAT ATTGATTCAG CTTGGTTGTG GCCATATGAA 
GAGACCATCA GGAAGTGTGC TCGCAGCTGG GTCACTGTTA TCAGACTGAT GGAAAAGAAC 
CCAGAGTTTG TCTTCACATG CTCCCAAGCA CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC 
CCGGGCCTGT TCTCAGAGAT CAAGCACTTT ATACAGAAAG GCCAGTTTAT TCCAGTTGGA 
GGCACTTGGG TAGAGATGGA TGGTAACTTG CCATCTGGTG AGTCCATGGT CAGACAGTTT 
CTACATGGCC AGAACTTCTT CAAGGACGAA TTTGGAAATT ACTGTAAAGA GTTTTGGCTA 
CCAGATACAT TTGGTTACTC TGCACAGCTG CCTCAGATCA TGAAAGGGTG TGGGATCTCT 
CGTTTTATTA CTCAGAAGCT GAGCTGGAAC CTTATTAACA CATTTCCACA CAACACATTC 
TTTTGGGAGG GTATTGACGG GACAGCAGTT CTCACTCATT TTCCTCCAGG TAACTCTTAT 
GAGATGAAGG GTAAAGTGGA AGACTTGATT AACACGGTGA AAAACAACAA AGACAAGGGA 
CGAGCAAATC ATAGTGCATT ATTGTTTGGT TTTGGAGACG GTGGTGGTGG ACCAACACAG 
CTAATGTTAG ACCGACTACA CCGTGTCAAG GATACAGATT GTCTGCCTAA GGTTCAGATG 
TCAACTCCTG ATGTCCTGTT TTCTCACCTG GAATCAGACT CATCGCTCCT TTGTAGATGG 
GTTGGTGAGC TCTTCCTTGA GCTACACAAT GGCACTTACA CTACACAAGC AAAGATCAAG 
ATGAAAAATC GACAATGTGA AACACTGCTT CATGATGTTG AGGTGGCAAG CTGTTTGGCC 
TTGTCTAAAA AGGATAGTTT CAAATACCCA GCATGCAGAC TTCAGGAACT GTGGAGACTG 
CTTCTACTCA ACCAGTTCCA TGACGTGATT CCTGGCAGCT GTATTGGGAT GGTTGTCGAG 
GATGCATTAC AGTACTATGA CGAAATACAC ACCACGGCAT CCAGACTTCT TCTTGATGCT 
TGCAAATCAC TGCCGTCCTC TCCTGACAAT GGTATTGCTG TGCTGAACAC TTTATCTTGG 
GAGAGAACTG AAGTCATATC ACTACCAATG AAGGACACAG AAACAAGATT AGCCTTGGTG 
AGAGTCCCTA GCGTGGGTGT AGCTCATGTC AAGGAGACAG CAGAGTCTGC TGAGTCTGTT 
ATTGCCACAA TGATGGTCAA TGGAACCATC ATTATGGAAA ACGGTCTTTT GAAGGCAACT 
ATAGACCATC TAGGCCGTTT GATGTCACTG CTTTTAATCC AAACAAACAG GGAAACCATT 
TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA TTGTTTGATG ATGTACCTTT GTATTGGGAT 
GCTTGGGATG TGATGGACTA CCATCTTCAA ACAAGGAAGC CTGTAATTGA GGTCATTGAA 
CCTGCCAAGG TGCTAAAATC AGGAGGAATT CAGGCCAGTG TCTCTTTCTC ACTAAGGATT 
AACGGAAAAA GCACTATTCA ACAGGAGATT GTTCTAGATG CCTGCTGTCC CTATATCAAA 
TTCAAAACAC ATGTGGTCTG GGAAGAGGCG CATAAGTTTC TAAAGGTAGA GTTCCCTGTG 
CAGGTGCGCA GCCCAGAGGC TACCTACGAG ATACAGTTTG GACACTTGCA GAGGCCTACT 
CATAGAAATA CCTCCTGGGA CTGGGCTCGT TTTGAGGTGT GGGGACACAA GTGGGCTGAT 
CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT CTTAACGACT CCAAATATGG CTACTCTGTC 
CATCAAAACA TCATGACCTT GTCCCTTTTG CGGGCTCCAA AAGCTCCAGA TCTCAATGCT 
GATATGGGGG ATCATATTTT TAGCTATGCG TTGATGCCAC ATACAGGCAC CTTTCAGGAA 
TCTGCAGTTA TCCAGTATGC CTACAACTTT AACTACCCGC TTCGTGTCCA TCATGGTGTC 
ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA AGCTCACCTG CTATCATCCT GGAAACCATA 
AAAAAGGCGG AAAAAAGAGA GAATGCCCTT CTTATTCGCC TCTATGAGTC TCACGGAAGC 
CGTGTGACTG TGACATTGTC AACTTCCTTT CTTTTACAAG AAGTCTGGCA TTGTGATCTC 
TTGGAGCGTC CAGATTTCAG AATTCCTGTG TCAGAGACAA CCTCTGGAAT ATCCTTAACA 
TTCAAACCAT TTCAAATCAG ATCTCTCCTC TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296368.1
CDS	92..3196
Translation	MYHQPVLKNRRTLLERVEKFISDIYFTDCNLRGRLFGDIYPLESISVFLSEKRIPYSEAIQQNFQPCKVGDSFGPTWWTCWFKVNLKIPETWGGKEVHLRWESDGEGMVWQNQQPVQGLTNEGEKTSYILTECLKDSESHGLVEICSIDKIITLYVELACNGLFGAGQGSMIAAPDPNRKFTLQKAELVVFNRDVMELLTDFEMLLDMVKVLGEGEQRGFQALFTANEMVNLCDPANPGSFAAARSLAQKFFAQKNGESQHVVHAMGHCHIDSAWLWPYEETIRKCARSWVTVIRLMEKNPEFVFTCSQAQQFQWVKSWYPGLFSEIKHFIQKGQFIPVGGTWVEMDGNLPSGESMVRQFLHGQNFFKDEFGNYCKEFWLPDTFGYSAQLPQIMKGCGISRFITQKLSWNLINTFPHNTFFWEGIDGTAVLTHFPPGNSYEMKGKVEDLINTVKNNKDKGRANHSALLFGFGDGGGGPTQLMLDRLHRVKDTDCLPKVQMSTPDVLFSHLESDSSLLCRWVGELFLELHNGTYTTQAKIKMKNRQCETLLHDVEVASCLALSKKDSFKYPACRLQELWRLLLLNQFHDVIPGSCIGMVVEDALQYYDEIHTTASRLLLDACKSLPSSPDNGIAVLNTLSWERTEVISLPMKDTETRLALVRVPSVGVAHVKETAESAESVIATMMVNGTIIMENGLLKATIDHLGRLMSLLLIQTNRETISEGCFGNQFALFDDVPLYWDAWDVMDYHLQTRKPVIEVIEPAKVLKSGGIQASVSFSLRINGKSTIQQEIVLDACCPYIKFKTHVVWEEAHKFLKVEFPVQVRSPEATYEIQFGHLQRPTHRNTSWDWARFEVWGHKWADLSEHGFGVALLNDSKYGYSVHQNIMTLSLLRAPKAPDLNADMGDHIFSYALMPHTGTFQESAVIQYAYNFNYPLRVHHGVIPKPWSAFSVSSPAIILETIKKAEKRENALLIRLYESHGSRVTVTLSTSFLLQEVWHCDLLERPDFRIPVSETTSGISLTFKPFQIRSLLLIIQ

Target ORF information:

RefSeq Version	XM_009298093.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mannosidase, alpha, class 2C, member 1 (man2c1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298093.3

ORF Insert Sequence:

ATGTACCACC AACCCGTACT GAAAAACAGA CGGACTCTGC TGGAGCGTGT GGAAAAGTTT 
ATTTCTGATA TATATTTCAC AGACTGCAAC CTTAGAGGAA GACTGTTTGG AGACATTTAC 
CCTCTTGAAT CCATCTCTGT GTTCTTGTCA GAGAAGCGCA TTCCTTATTC AGAGGCAATT 
CAACAAAACT TTCAGCCTTG TAAAGTTGGT GATTCATTTG GACCCACCTG GTGGACATGC 
TGGTTCAAAG TAAACTTGAA GATCCCAGAG ACTTGGGGAG GGAAGGAAGT CCACTTAAGA 
TGGGAAAGTG ATGGAGAAGG AATGGTATGG CAGAATCAAC AACCAGTTCA GGGATTGACC 
AATGAAGGTG AGAAGACAAG TTACATTCTG ACTGAGTGCT TGAAGGACAG TGAATCTCAT 
GGGTTAGTGG AAATATGCAG TATAGACAAA ATAATTACTC TATATGTGGA ACTTGCATGT 
AATGGACTTT TTGGAGCTGG ACAAGGTTCC ATGATTGCCG CCCCTGACCC AAACAGAAAG 
TTCACTCTAC AGAAGGCAGA GCTTGTTGTA TTCAATCGAG ATGTCATGGA ACTTCTAACT 
GATTTTGAGA TGCTTCTGGA CATGGTCAAG GTACTAGGAG AAGGAGAGCA ACGTGGGTTT 
CAGGCTTTGT TTACAGCCAA TGAAATGGTG AATCTGTGTG ATCCAGCCAA TCCTGGTTCA 
TTTGCTGCTG CTCGAAGTCT AGCTCAGAAG TTTTTTGCCC AGAAAAATGG GGAAAGCCAA 
CATGTGGTAC ATGCTATGGG ACACTGCCAT ATTGATTCAG CTTGGTTGTG GCCATATGAA 
GAGACCATCA GGAAGTGTGC TCGCAGCTGG GTCACTGTTA TCAGACTGAT GGAAAAGAAC 
CCAGAGTTTG TCTTCACATG CTCCCAAGCA CAGCAGTTTC AGTGGGTGAA GAGCTGGTAC 
CCGGGCCTGT TCTCAGAGAT CAAGCACTTT ATACAGAAAG GCCAGTTTAT TCCAGTTGGA 
GGCACTTGGG TAGAGATGGA TGGTAACTTG CCATCTGGTG AGTCCATGGT CAGACAGTTT 
CTACATGGCC AGAACTTCTT CAAGGACGAA TTTGGAAATT ACTGTAAAGA GTTTTGGCTA 
CCAGATACAT TTGGTTACTC TGCACAGCTG CCTCAGATCA TGAAAGGGTG TGGGATCTCT 
CGTTTTATTA CTCAGAAGCT GAGCTGGAAC CTTATTAACA CATTTCCACA CAACACATTC 
TTTTGGGAGG GTATTGACGG GACAGCAGTT CTCACTCATT TTCCTCCAGG TAACTCTTAT 
GAGATGAAGG GTAAAGTGGA AGACTTGATT AACACGGTGA AAAACAACAA AGACAAGGGA 
CGAGCAAATC ATAGTGCATT ATTGTTTGGT TTTGGAGACG GTGGTGGTGG ACCAACACAG 
CTAATGTTAG ACCGACTACA CCGTGTCAAG GATACAGATT GTCTGCCTAA GGTTCAGATG 
TCAACTCCTG ATGTCCTGTT TTCTCACCTG GAATCAGACT CATCGCTCCT TTGTAGATGG 
GTTGGTGAGC TCTTCCTTGA GCTACACAAT GGCACTTACA CTACACAAGC AAAGATCAAG 
ATGAAAAATC GACAATGTGA AACACTGCTT CATGATGTTG AGGTGGCAAG CTGTTTGGCC 
TTGTCTAAAA AGGATAGTTT CAAATACCCA GCATGCAGAC TTCAGGAACT GTGGAGACTG 
CTTCTACTCA ACCAGTTCCA TGACGTGATT CCTGGCAGCT GTATTGGGAT GGTTGTCGAG 
GATGCATTAC AGTACTATGA CGAAATACAC ACCACGGCAT CCAGACTTCT TCTTGATGCT 
TGCAAATCAC TGCCGTCCTC TCCTGACAAT GGTATTGCTG TGCTGAACAC TTTATCTTGG 
GAGAGAACTG AAGTCATATC ACTACCAATG AAGGACACAG AAACAAGATT AGCCTTGGTG 
AGAGTCCCTA GCGTGGGTGT AGCTCATGTC AAGGAGACAG CAGAGTCTGC TGAGTCTGTT 
ATTGCCACAA TGATGGTCAA TGGAACCATC ATTATGGAAA ACGGTCTTTT GAAGGCAACT 
ATAGACCATC TAGGCCGTTT GATGTCACTG CTTTTAATCC AAACAAACAG GGAAACCATT 
TCCGAGGGTT GCTTTGGGAA TCAGTTTGCA TTGTTTGATG ATGTACCTTT GTATTGGGAT 
GCTTGGGATG TGATGGACTA CCATCTTCAA ACAAGGAAGC CTGTAATTGA GGTCATTGAA 
CCTGCCAAGG TGCTAAAATC AGGAGGAATT CAGGCCAGTG TCTCTTTCTC ACTAAGGATT 
AACGGAAAAA GCACTATTCA ACAGGAGATT GTTCTAGATG CCTGCTGTCC CTATATCAAA 
TTCAAAACAC ATGTGGTCTG GGAAGAGGCG CATAAGTTTC TAAAGGTAGA GTTCCCTGTG 
CAGGTGCGCA GCCCAGAGGC TACCTACGAG ATACAGTTTG GACACTTGCA GAGGCCTACT 
CATAGAAATA CCTCCTGGGA CTGGGCTCGT TTTGAGGTGT GGGGACACAA GTGGGCTGAT 
CTGTCCGAGC ATGGTTTTGG CGTGGCTCTT CTTAACGACT CCAAATATGG CTACTCTGTC 
CATCAAAACA TCATGACCTT GTCCCTTTTG CGGGCTCCAA AAGCTCCAGA TCTCAATGCT 
GATATGGGGG ATCATATTTT TAGCTATGCG TTGATGCCAC ATACAGGCAC CTTTCAGGAA 
TCTGCAGTTA TCCAGTATGC CTACAACTTT AACTACCCGC TTCGTGTCCA TCATGGTGTC 
ATTCCTAAAC CCTGGAGTGC ATTCTCCGTA AGCTCACCTG CTATCATCCT GGAAACCATA 
AAAAAGGCGG AAAAAAGAGA GAATGCCCTT CTTATTCGCC TCTATGAGTC TCACGGAAGC 
CGTGTGACTG TGACATTGTC AACTTCCTTT CTTTTACAAG AAGTCTGGCA TTGTGATCTC 
TTGGAGCGTC CAGATTTCAG AATTCCTGTG TCAGAGACAA CCTCTGGAAT ATCCTTAACA 
TTCAAACCAT TTCAAATCAG ATCTCTCCTC TTGATTATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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man2c1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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