mcf2lb gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021478971.1	XP_021334646.1	guanine nucleotide exchange factor DBS isoform X2
XM_021478972.1	XP_021334647.1	guanine nucleotide exchange factor DBS isoform X3
XM_009304775.2	XP_009303050.1	guanine nucleotide exchange factor DBS isoform X1
XM_009304776.2	XP_009303051.1	guanine nucleotide exchange factor DBS isoform X3
XM_021478973.1	XP_021334648.1	guanine nucleotide exchange factor DBS isoform X4
XM_021472200.1	XP_021327875.1	guanine nucleotide exchange factor DBS isoform X2
XM_009304777.2	XP_009303052.1	guanine nucleotide exchange factor DBS isoform X4
XM_021478974.1	XP_021334649.1	guanine nucleotide exchange factor DBS isoform X5
XM_009304777.3	XP_009303052.1	guanine nucleotide exchange factor DBS isoform X5
XM_692603.7	XP_697695.5	guanine nucleotide exchange factor DBS isoform X2
XM_009304776.3	XP_009303051.1	guanine nucleotide exchange factor DBS isoform X4
XM_021478970.1	XP_021334645.1	guanine nucleotide exchange factor DBS isoform X1
XM_009304775.3	XP_009303050.1	guanine nucleotide exchange factor DBS isoform X1
XM_692603.8	XP_697695.5	guanine nucleotide exchange factor DBS isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144085	XM_021472200.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa144085	XM_021478971.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa15308	XM_692603.8 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15308	XM_021478972.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45701	XM_009304775.3 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45701	XM_021478970.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45702	XM_009304776.3 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45702	XM_021478973.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45703	XM_021478974.1 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45703	XM_009304777.3 Latest version!	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa45703	XM_009304777.2	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45702	XM_009304776.2	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45701	XM_009304775.2	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15308	XM_692603.7	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

mcf2lb ( XM_021478971.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334646.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa144085
Clone ID Related Accession (Same CDS sequence)	XM_021478971.1 , XM_021472200.1
Accession Version	XM_021478971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGGCCACACC CTAACTTGGG CGGAGTCAGG 
TGGTTCAGTA CATCAAGCCT GTTTCAGAGT CGTAGGAGAG GTTGGATGAA AGGATCTCTC 
TCGCTGGATG CCTCTGTGGA GCATGATGGA TATTTCAGTG CAGAAGAGCA GGCCAACTCT 
GACCCAGAAG AGGAGAGGGA AGACAAACTG AGTGAAGAAG AACCGCAGCC TGTGAGGATA 
GATGAAGAGA TCCAGTGTTT TGAAGAGACA GAGGAACCTG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334646.1
CDS	58..3345
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021478971.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478971.1

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGGCCACACC CTAACTTGGG CGGAGTCAGG 
TGGTTCAGTA CATCAAGCCT GTTTCAGAGT CGTAGGAGAG GTTGGATGAA AGGATCTCTC 
TCGCTGGATG CCTCTGTGGA GCATGATGGA TATTTCAGTG CAGAAGAGCA GGCCAACTCT 
GACCCAGAAG AGGAGAGGGA AGACAAACTG AGTGAAGAAG AACCGCAGCC TGTGAGGATA 
GATGAAGAGA TCCAGTGTTT TGAAGAGACA GAGGAACCTG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_021472200.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327875.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa144085
Clone ID Related Accession (Same CDS sequence)	XM_021478971.1 , XM_021472200.1
Accession Version	XM_021472200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394759.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGGCCACACC CTAACTTGGG CGGAGTCAGG 
TGGTTCAGTA CATCAAGCCT GTTTCAGAGT CGTAGGAGAG GTTGGATGAA AGGATCTCTC 
TCGCTGGATG CCTCTGTGGA GCATGATGGA TATTTCAGTG CAGAAGAGCA GGCCAACTCT 
GACCCAGAAG AGGAGAGGGA AGACAAACTG AGTGAAGAAG AACCGCAGCC TGTGAGGATA 
GATGAAGAGA TCCAGTGTTT TGAAGAGACA GAGGAACCTG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327875.1
CDS	58..3345
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021472200.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472200.1

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGGCCACACC CTAACTTGGG CGGAGTCAGG 
TGGTTCAGTA CATCAAGCCT GTTTCAGAGT CGTAGGAGAG GTTGGATGAA AGGATCTCTC 
TCGCTGGATG CCTCTGTGGA GCATGATGGA TATTTCAGTG CAGAAGAGCA GGCCAACTCT 
GACCCAGAAG AGGAGAGGGA AGACAAACTG AGTGAAGAAG AACCGCAGCC TGTGAGGATA 
GATGAAGAGA TCCAGTGTTT TGAAGAGACA GAGGAACCTG AGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_021478972.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334647.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa15308
Clone ID Related Accession (Same CDS sequence)	XM_021478972.1 , XM_692603.7 , XM_692603.8
Accession Version	XM_021478972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334647.1
CDS	185..3466
Translation	MAMIWMQTEKTVVQELQRRLSNVAHSIDEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021478972.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478972.1

ORF Insert Sequence:

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_692603.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697695.5 Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15308
Clone ID Related Accession (Same CDS sequence)	XM_021478972.1 , XM_692603.7 , XM_692603.8
Accession Version	XM_692603.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_692603.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697695.5
CDS	293..3574
Translation	MAMIWMQTEKTVVQELQRRLSNVAHSIDEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_692603.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692603.7

ORF Insert Sequence:

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_692603.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697695.5 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.

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CloneID	ODa15308
Clone ID Related Accession (Same CDS sequence)	XM_021478972.1 , XM_692603.7 , XM_692603.8
Accession Version	XM_692603.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394759.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_692603.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697695.5
CDS	185..3466
Translation	MAMIWMQTEKTVVQELQRRLSNVAHSIDEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_692603.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692603.8

ORF Insert Sequence:

ATGGCGATGA TCTGGATGCA GACAGAGAAG ACGGTCGTGC AAGAGCTGCA GCGCAGATTG 
AGCAATGTCG CTCACAGCAT TGATGAAATC ATGCAGAAGG AGTCGAGTCC CCTCTTCGCT 
GCTGATGTCA TCACTGAACT CAAGAGACAG TTTGCGTTCC TGTCTGGTGG AAGAGCACAG 
GATGGAAGCC CTATCATTAT CTTCCCAGAA TACCCTTCAT TTTGTGAAAT AGGAGACCAA 
GAGTTTCGTA ATGTGCTTAC ATACCTGACA AGTATTCCCA GTCTGAGTGC AGCAGGTGTG 
GGCTTTATCG TAGTGATTGA CAGACGTCAA GATCGATGGA CGTGTTTGAA GGGAACATTG 
TTGCGTATTT CAGGCTGGTT TCCTGCTAAT CTTCGGCTTG TTCTGGTTCT GAGGCCCAGT 
GCTATACTGC AGCGAACACT TTCTGATGTC TTTTTCAAAC TCCACAGAGA TGACTTTAAA 
GTACCGGTTA TCATGCTAAG CTCAGTGGCT GATCTCCATA CTTATATTGA GAGGAATCAA 
CTGACACAGG AACTTGGCGG TTCTCAGTAC TACTGCCACA AAACCTGGAT CTCTCACCGC 
ACAGATCTTG AAAGCTTCGC TGCGTTAGTG AAGAGGATGG CTCAGAGACT GCAAGTGTTC 
GGCAGAGAGC TGGCAGAAAC CGAGCTACCC AGCAACCACC TGACAGCCAG CAGTCTGCTT 
AATACACACA CCAGCAGAAA AGACATCTTC AAAGAAGACA TGGCAGGAGC TCTCAGCCAA 
GGTCGGAAAA TCTTGGAGAA TATCAGAGAG CCGGTGCGCA GAGATCCGGA CAGCAGTTTG 
AACCCTGACC AGTTGGAGAA TCTTGCTACA GTCCACAGGC TCTTATCCCA GCTTAATGAG 
AGCTCAACAG CATTCGATGA GTTCTGGATT CAGCATCAAA ATAAACTGGA GCTATGTCTG 
AAAGTCTGTC AGTTTGAGCA CAACTTTCAG CAGTTGCGAA CAGAGCTGGA TCGAGCAACT 
GAAACTCTGA ATGCTTTCTC AGCTGTTGGA ATAAGCCCCG CCCAAACAGA ACACCTCCTT 
CAAGAGCTGA CCAATCATGA AAAGAAAGTC TGTGAAGTGC TGGACAGAGT GAGGTCTCTG 
GTTGCTGAGG GTCAAGGTTT GATTGACAGC TCTCAGATTC TTGATGACAG TATTGCAGCA 
AAATGCAGTG AGCTAGAAAA AGCAAGTGAA AATCTCACCC AAGAGCTTAA AGACAAGCAG 
ACCAAGCTGA CACAGGCTAT GGATCTACAT AAAAGACTGG ACATGATGTG TAAATGGTGT 
GATGATGGGA TTTACATGCT GGCATCACAA CCACTGGACA AGTGCCAGTC ACAGGAGGGG 
GCAGAGTCAG CGCTATCAGA GCTAGAATGT TACCTGGAGA CAGCCAATGA GAAACAGAAG 
TGGGAAATCA GCACAGTTTG GCAGGAATAT GACAACATAT TGAACAATGA GCTTAGGGAG 
CAAGTGACGC GTGCATTTGA GAAGAAAGCC TCACTACAGG AGATGTTTGA TAGAAGGAGG 
GTCAGTCTGA AGAAACTAGC AGCCAAACAA ACACGTCCTG TACAACCTGT GGCTCCCAGT 
CCAGAGTCAC TCTCATCTCC TGCTCACAGG GATCATGAGA GCATCTGTAT TAGTGAGGAC 
TCGGACAGCA GGGGCTCCTG TAAACAAATA AACTCTGATC AGGTTGACAG GAGCAGTCGC 
AATGCTTCTG TATCTGAGGA GGAAGAAACC CTGGCTGTAC TGCGCAGGCA TGTAATGAAC 
GAGTTATTGG AAACCGAGAG AGCATATGTG GAAGAGCTTA TGTGTGTGTT GCAGGGATAT 
GCTGCTGAGA TGGACAATCC CTCCATGGCT CCTCTTATTC CTGCTGCCCT GCAGAACAAG 
AAGGACGTGT TATTTGGGAA CATGCAAGAG ATATACAATT TCCACAAAAG AATATTTCTC 
AGAGAGCTGG AGACCTACAC TGACTATCCT GAGCTTGTGG GCCGCTGCTT CCTGGACTGG 
ATGGGGGAGC TGCAAATTTA TGAGAAATAC TGTCACAACA AACCTCGTTC TGAGAGTCTC 
TGGAGACAGT GCTCCGACTG CGCCTTTTTC CAGGAGTGTC AGAAGAAACT AGAACACAAG 
CTGGGACTGG ACTCTTACTT ACTGAAGCCT GTACAGAGGA TCACTAAATA CCAACTTCTG 
ATTAAGGAGA TGATTAAATA CAGTAAAGGC TGTGAGGGCT CAGTGGAGCT GCAGGCGGCT 
CTTTCCTCAA TACTGGGAAT CCTGAAGGCC GTAAATGACT CAATGCACCT CATCGCCATC 
ACAGGATATG ATGGTAATCT TGGAGACCTG GGTCGTCTGC TGATGCAGGG GTCGTTCAGC 
GTGTGGGCAG AGCACAAGAG AGGTCATGTG AAGGTGATGG AGCTCGCCAG GTTTAAGCCC 
ATGCAGAGAC ACCTGTTCCT GCATGAGAAA GCTCTGCTCT TCTGCAAGAG ACGAGAGGAG 
AGCGGAGAGG GATACGAAAA AGCCCCTTCA TACAGCTTTA AACAGGAGCT CAGCATGGCT 
GCTATTGGAA TAACAGAGCA TGCTAAAGGG GACAGCAAGA AGTTTGAAAT CTGGTCCAGT 
TCAAGAGACG AGGTGTACAC GATACAGGCT GCATCTGAAG AGGTTAAGAC CATCTGGGTG 
ACAGAAATCC GCAAACTTCT AACAGGACAA CTGGAAGCCT GCAAAGAAAG GATCTTTGAG 
GAACCAAAAA ATGAAGCAAG TCAGCAGAGG GCACCTGAGC AGTTCACCTC AGAGAGCAGC 
TCAACAAACA GACTGGTGAG AAATGAGCGT AGTAGCCTCA AGAGTGACGG AGTGGAGAAA 
CAAAAAAGAG AAGAGGAAAG AGGCAAGGAA CTTGAGAGCA TTTTTGAATC GAGGACAGCC 
GAAAGGGCAA AAGGATCTTC GTTCAGCTTT GAGACAAAAC CAAAACGGCA GGATGTCCGG 
AGCGACCCTA CGCCTCTAGA AACAGGGCCA CACCCTAACT TGGGCGGAGT CAGGTGGTTC 
AGTACATCAA GCCTGTTTCA GAGTCGTAGG AGAGGTTGGA TGAAAGGATC TCTCTCGCTG 
GATGCCTCTG TGGAGCATGA TGGATATTTC AGTGCAGAAG AGCAGGCCAA CTCTGACCCA 
GAAGAGGAGA GGGAAGACAA ACTGAGTGAA GAAGAACCGC AGCCTGTGAG GATAGATGAA 
GAGATCCAGT GTTTTGAAGA GACAGAGGAA CCTGAGGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304775.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303050.1 Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X1, mRNA.

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CloneID	ODa45701
Clone ID Related Accession (Same CDS sequence)	XM_009304775.2 , XM_021478970.1 , XM_009304775.3
Accession Version	XM_009304775.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303050.1
CDS	65..3358
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304775.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304775.2

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_021478970.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334645.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45701
Clone ID Related Accession (Same CDS sequence)	XM_009304775.2 , XM_021478970.1 , XM_009304775.3
Accession Version	XM_021478970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334645.1
CDS	57..3350
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021478970.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478970.1

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304775.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303050.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.

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CloneID	ODa45701
Clone ID Related Accession (Same CDS sequence)	XM_009304775.2 , XM_021478970.1 , XM_009304775.3
Accession Version	XM_009304775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394759.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303050.1
CDS	57..3350
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304775.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304775.3

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GAAACAGGGC CACACCCTAA CTTGGGCGGA 
GTCAGGTGGT TCAGTACATC AAGCCTGTTT CAGAGTCGTA GGAGAGGTTG GATGAAAGGA 
TCTCTCTCGC TGGATGCCTC TGTGGAGCAT GATGGATATT TCAGTGCAGA AGAGCAGGCC 
AACTCTGACC CAGAAGAGGA GAGGGAAGAC AAACTGAGTG AAGAAGAACC GCAGCCTGTG 
AGGATAGATG AAGAGATCCA GTGTTTTGAA GAGACAGAGG AACCTGAGGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304776.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303051.1 Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45702
Clone ID Related Accession (Same CDS sequence)	XM_009304776.2 , XM_021478973.1 , XM_009304776.3
Accession Version	XM_009304776.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303051.1
CDS	64..3330
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304776.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304776.2

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_021478973.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334648.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.

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CloneID	ODa45702
Clone ID Related Accession (Same CDS sequence)	XM_009304776.2 , XM_021478973.1 , XM_009304776.3
Accession Version	XM_021478973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334648.1
CDS	57..3323
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021478973.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478973.1

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304776.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303051.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa45702
Clone ID Related Accession (Same CDS sequence)	XM_009304776.2 , XM_021478973.1 , XM_009304776.3
Accession Version	XM_009304776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394759.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303051.1
CDS	57..3323
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304776.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304776.3

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
GCAAGTCAGC AGAGGGCACC TGAGCAGTTC ACCTCAGAGA GCAGCTCAAC AAACAGACTG 
GTGAGAAATG AGCGTAGTAG CCTCAAGAGT GACGGAGTGG AGAAACAAAA AAGAGAAGAG 
GAAAGAGGCA AGGAACTTGA GAGCATTTTT GAATCGAGGA CAGCCGAAAG GGCAAAAGGA 
TCTTCGTTCA GCTTTGAGAC AAAACCAAAA CGGCAGGATG TCCGGAGCGA CCCTACGCCT 
CTAGAAACAG GGCCACACCC TAACTTGGGC GGAGTCAGGT GGTTCAGTAC ATCAAGCCTG 
TTTCAGAGTC GTAGGAGAGG TTGGATGAAA GGATCTCTCT CGCTGGATGC CTCTGTGGAG 
CATGATGGAT ATTTCAGTGC AGAAGAGCAG GCCAACTCTG ACCCAGAAGA GGAGAGGGAA 
GACAAACTGA GTGAAGAAGA ACCGCAGCCT GTGAGGATAG ATGAAGAGAT CCAGTGTTTT 
GAAGAGACAG AGGAACCTGA GGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304777.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303052.1 Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa45703
Clone ID Related Accession (Same CDS sequence)	XM_009304777.2 , XM_021478974.1 , XM_009304777.3
Accession Version	XM_009304777.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303052.1
CDS	64..3282
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304777.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio guanine nucleotide exchange factor DBS (LOC569231), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304777.2

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_021478974.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334649.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa45703
Clone ID Related Accession (Same CDS sequence)	XM_009304777.2 , XM_021478974.1 , XM_009304777.3
Accession Version	XM_021478974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334649.1
CDS	57..3275
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_021478974.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478974.1

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2lb ( XM_009304777.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303052.1 Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa45703
Clone ID Related Accession (Same CDS sequence)	XM_009304777.2 , XM_021478974.1 , XM_009304777.3
Accession Version	XM_009304777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394759.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303052.1
CDS	57..3275
Translation	MDVQNRRKTICIPLAEVEKYYHFSRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIVVIDRRQDRWTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLSSVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRKDIFKEDMAGALSQGRKILENIREPVRRDPDSSLNPDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELCLKVCQFEHNFQQLRTELDRATETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVRSLVAEGQGLIDSSQILDDSIAAKCSELEKASENLTQELKDKQTKLTQAMDLHKRLDMMCKWCDDGIYMLASQPLDKCQSQEGAESALSELECYLETANEKQKWEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLSSPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYVEELMCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETYTDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPVQRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQGSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAAIGITEHAKGDSKKFEIWSSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPEQFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGKELESIFESRTAERAKGSSFSFETKPKRQDVRSDPTPLGWMKGSLSLDASVEHDGYFSAEEQANSDPEEEREDKLSEEEPQPVRIDEEIQCFEETEEPEE

Target ORF information:

RefSeq Version	XM_009304777.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mcf.2 cell line derived transforming sequence-like b (mcf2lb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304777.3

ORF Insert Sequence:

ATGGATGTAC AGAACAGAAG GAAAACTATA TGCATTCCTC TAGCAGAGGT TGAGAAATAT 
TATCACTTTT CCAGACGTTG CCAATGGCTA CAAAATGAAA TCATGCAGAA GGAGTCGAGT 
CCCCTCTTCG CTGCTGATGT CATCACTGAA CTCAAGAGAC AGTTTGCGTT CCTGTCTGGT 
GGAAGAGCAC AGGATGGAAG CCCTATCATT ATCTTCCCAG AATACCCTTC ATTTTGTGAA 
ATAGGAGACC AAGAGTTTCG TAATGTGCTT ACATACCTGA CAAGTATTCC CAGTCTGAGT 
GCAGCAGGTG TGGGCTTTAT CGTAGTGATT GACAGACGTC AAGATCGATG GACGTGTTTG 
AAGGGAACAT TGTTGCGTAT TTCAGGCTGG TTTCCTGCTA ATCTTCGGCT TGTTCTGGTT 
CTGAGGCCCA GTGCTATACT GCAGCGAACA CTTTCTGATG TCTTTTTCAA ACTCCACAGA 
GATGACTTTA AAGTACCGGT TATCATGCTA AGCTCAGTGG CTGATCTCCA TACTTATATT 
GAGAGGAATC AACTGACACA GGAACTTGGC GGTTCTCAGT ACTACTGCCA CAAAACCTGG 
ATCTCTCACC GCACAGATCT TGAAAGCTTC GCTGCGTTAG TGAAGAGGAT GGCTCAGAGA 
CTGCAAGTGT TCGGCAGAGA GCTGGCAGAA ACCGAGCTAC CCAGCAACCA CCTGACAGCC 
AGCAGTCTGC TTAATACACA CACCAGCAGA AAAGACATCT TCAAAGAAGA CATGGCAGGA 
GCTCTCAGCC AAGGTCGGAA AATCTTGGAG AATATCAGAG AGCCGGTGCG CAGAGATCCG 
GACAGCAGTT TGAACCCTGA CCAGTTGGAG AATCTTGCTA CAGTCCACAG GCTCTTATCC 
CAGCTTAATG AGAGCTCAAC AGCATTCGAT GAGTTCTGGA TTCAGCATCA AAATAAACTG 
GAGCTATGTC TGAAAGTCTG TCAGTTTGAG CACAACTTTC AGCAGTTGCG AACAGAGCTG 
GATCGAGCAA CTGAAACTCT GAATGCTTTC TCAGCTGTTG GAATAAGCCC CGCCCAAACA 
GAACACCTCC TTCAAGAGCT GACCAATCAT GAAAAGAAAG TCTGTGAAGT GCTGGACAGA 
GTGAGGTCTC TGGTTGCTGA GGGTCAAGGT TTGATTGACA GCTCTCAGAT TCTTGATGAC 
AGTATTGCAG CAAAATGCAG TGAGCTAGAA AAAGCAAGTG AAAATCTCAC CCAAGAGCTT 
AAAGACAAGC AGACCAAGCT GACACAGGCT ATGGATCTAC ATAAAAGACT GGACATGATG 
TGTAAATGGT GTGATGATGG GATTTACATG CTGGCATCAC AACCACTGGA CAAGTGCCAG 
TCACAGGAGG GGGCAGAGTC AGCGCTATCA GAGCTAGAAT GTTACCTGGA GACAGCCAAT 
GAGAAACAGA AGTGGGAAAT CAGCACAGTT TGGCAGGAAT ATGACAACAT ATTGAACAAT 
GAGCTTAGGG AGCAAGTGAC GCGTGCATTT GAGAAGAAAG CCTCACTACA GGAGATGTTT 
GATAGAAGGA GGGTCAGTCT GAAGAAACTA GCAGCCAAAC AAACACGTCC TGTACAACCT 
GTGGCTCCCA GTCCAGAGTC ACTCTCATCT CCTGCTCACA GGGATCATGA GAGCATCTGT 
ATTAGTGAGG ACTCGGACAG CAGGGGCTCC TGTAAACAAA TAAACTCTGA TCAGGTTGAC 
AGGAGCAGTC GCAATGCTTC TGTATCTGAG GAGGAAGAAA CCCTGGCTGT ACTGCGCAGG 
CATGTAATGA ACGAGTTATT GGAAACCGAG AGAGCATATG TGGAAGAGCT TATGTGTGTG 
TTGCAGGGAT ATGCTGCTGA GATGGACAAT CCCTCCATGG CTCCTCTTAT TCCTGCTGCC 
CTGCAGAACA AGAAGGACGT GTTATTTGGG AACATGCAAG AGATATACAA TTTCCACAAA 
AGAATATTTC TCAGAGAGCT GGAGACCTAC ACTGACTATC CTGAGCTTGT GGGCCGCTGC 
TTCCTGGACT GGATGGGGGA GCTGCAAATT TATGAGAAAT ACTGTCACAA CAAACCTCGT 
TCTGAGAGTC TCTGGAGACA GTGCTCCGAC TGCGCCTTTT TCCAGGAGTG TCAGAAGAAA 
CTAGAACACA AGCTGGGACT GGACTCTTAC TTACTGAAGC CTGTACAGAG GATCACTAAA 
TACCAACTTC TGATTAAGGA GATGATTAAA TACAGTAAAG GCTGTGAGGG CTCAGTGGAG 
CTGCAGGCGG CTCTTTCCTC AATACTGGGA ATCCTGAAGG CCGTAAATGA CTCAATGCAC 
CTCATCGCCA TCACAGGATA TGATGGTAAT CTTGGAGACC TGGGTCGTCT GCTGATGCAG 
GGGTCGTTCA GCGTGTGGGC AGAGCACAAG AGAGGTCATG TGAAGGTGAT GGAGCTCGCC 
AGGTTTAAGC CCATGCAGAG ACACCTGTTC CTGCATGAGA AAGCTCTGCT CTTCTGCAAG 
AGACGAGAGG AGAGCGGAGA GGGATACGAA AAAGCCCCTT CATACAGCTT TAAACAGGAG 
CTCAGCATGG CTGCTATTGG AATAACAGAG CATGCTAAAG GGGACAGCAA GAAGTTTGAA 
ATCTGGTCCA GTTCAAGAGA CGAGGTGTAC ACGATACAGG CTGCATCTGA AGAGGTTAAG 
ACCATCTGGG TGACAGAAAT CCGCAAACTT CTAACAGGAC AACTGGAAGC CTGCAAAGAA 
AGGATCTTTG AGGAACCAAA AAATGAAGCA AGTCAGCAGA GGGCACCTGA GCAGTTCACC 
TCAGAGAGCA GCTCAACAAA CAGACTGGTG AGAAATGAGC GTAGTAGCCT CAAGAGTGAC 
GGAGTGGAGA AACAAAAAAG AGAAGAGGAA AGAGGCAAGG AACTTGAGAG CATTTTTGAA 
TCGAGGACAG CCGAAAGGGC AAAAGGATCT TCGTTCAGCT TTGAGACAAA ACCAAAACGG 
CAGGATGTCC GGAGCGACCC TACGCCTCTA GGTTGGATGA AAGGATCTCT CTCGCTGGAT 
GCCTCTGTGG AGCATGATGG ATATTTCAGT GCAGAAGAGC AGGCCAACTC TGACCCAGAA 
GAGGAGAGGG AAGACAAACT GAGTGAAGAA GAACCGCAGC CTGTGAGGAT AGATGAAGAG 
ATCCAGTGTT TTGAAGAGAC AGAGGAACCT GAGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mcf2lb
Entrez Gene ID	569231
Full Name	mcf.2 cell line derived transforming sequence-like b
Synonyms	mcf2l,sb:eu830,si:dkey-46k9.10
General protein information	Preferred Names mcf.2 cell line derived transforming sequence-like b Names guanine nucleotide exchange factor DBS MCF.2 cell line derived transforming sequence-like guanine nucleotide exchange factor DBS-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mcf2lb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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