slc8a4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc8a4a
Entrez Gene ID	568512
Full Name	solute carrier family 8 (sodium/calcium exchanger), member 4a
General protein information	Preferred Names solute carrier family 8 (sodium/calcium exchanger), member 4a Names solute carrier family 8 (sodium/calcium exchanger), member 4a Ncx4a sodium/calcium exchanger 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161357.3	XP_005161414.1	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
XM_009295486.2	XP_009293761.1	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
XM_005161357.4	XP_005161414.1	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
NM_001089419.1	NP_001082888.1	solute carrier family 8 member 4a precursor
XM_021468890.1	XP_021324565.1	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
XM_009295486.3	XP_009293761.1	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	slc8a4b	NP_001116712.1
Oryza sativa (rice)	Os11g0660000	NP_001068402.1
Xenopus tropicalis (tropical clawed frog)	LOC100487899	XP_002940274.1
Arabidopsis thaliana (thale cress)	MHX	NP_566105.1
Danio rerio (zebrafish)	slc8a4a	NP_001082888.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The ?2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish.
Doganli C, Kjaer-Sorensen K, Knoeckel C, Beck HC, Nyengaard JR, Honor? B, Nissen P, Ribera A, Oxvig C, Lykke-Hartmann K
Journal of cell science125(Pt 24)6166-75(2012 Dec)

Effects of dietary methylmercury on the zebrafish brain: histological, mitochondrial, and gene transcription analyses.
Cambier S, Gonzalez P, Mesmer-Dudons N, Br?thes D, Fujimura M, Bourdineaud JP
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(1)165-80(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Characterization of zebrafish (Danio rerio) NCX4: a novel NCX with distinct electrophysiological properties.
Title: Characterization of zebrafish (Danio rerio) NCX4: a novel NCX with distinct electrophysiological properties.

Na,K-ATPase alpha2 and Ncx4a regulate left-right patterning by modulating intracellular calcium levels in Kupffer's vesicle and by influencing cilia function
Title: Na,K-ATPase alpha2 and Ncx4a regulate zebrafish left-right patterning.

The following slc8a4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc8a4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00359	NM_001089419.1 Latest version!	Danio rerio solute carrier family 8 member 4a (slc8a4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25974	XM_021468890.1 Latest version!	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25974	XM_009295486.3 Latest version!	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25974	XM_005161357.4 Latest version!	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa25974	XM_005161357.3	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa25974	XM_009295486.2	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc8a4a ( NM_001089419.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082888.1 Danio rerio solute carrier family 8 member 4a (slc8a4a), mRNA.

Price & Availability↑

CloneID	ODa00359
Clone ID Related Accession (Same CDS sequence)	NM_001089419.1
Accession Version	NM_001089419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	solute carrier family 8 member 4a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF470289.1. On May 24, 2007 this sequence version replaced XM_691845.2. ##Evidence-Data-START## Transcript exon combination :: EF470289.1, EU559290.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476810 [ECO:0000348] ##Evidence-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCCGGTGTG GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GAGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATT 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACGTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT AAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCCGTGTTG 
CTGGAGATCG GACAGAAACA CGGAGACTCT AATGATAACA AACCAGCGGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGTGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTCCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGCTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082888.1
CDS	1..2820
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	NM_001089419.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 8 member 4a (slc8a4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089419.1

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCCGGTGTG GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GAGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATT 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACGTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT AAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCCGTGTTG 
CTGGAGATCG GACAGAAACA CGGAGACTCT AATGATAACA AACCAGCGGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGTGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTCCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGCTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a4a ( XM_005161357.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161414.1 Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25974
Clone ID Related Accession (Same CDS sequence)	XM_005161357.3 , XM_009295486.2 , XM_005161357.4 , XM_021468890.1 , XM_009295486.3
Accession Version	XM_005161357.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161357.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161414.1
CDS	660..3479
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	XM_005161357.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161357.3

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a4a ( XM_009295486.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293761.1 Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25974
Clone ID Related Accession (Same CDS sequence)	XM_005161357.3 , XM_009295486.2 , XM_005161357.4 , XM_021468890.1 , XM_009295486.3
Accession Version	XM_009295486.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293761.1
CDS	568..3387
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	XM_009295486.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295486.2

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a4a ( XM_005161357.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161414.1 Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25974
Clone ID Related Accession (Same CDS sequence)	XM_005161357.3 , XM_009295486.2 , XM_005161357.4 , XM_021468890.1 , XM_009295486.3
Accession Version	XM_005161357.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161357.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161414.1
CDS	1020..3839
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	XM_005161357.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161357.4

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a4a ( XM_021468890.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324565.1 Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25974
Clone ID Related Accession (Same CDS sequence)	XM_005161357.3 , XM_009295486.2 , XM_005161357.4 , XM_021468890.1 , XM_009295486.3
Accession Version	XM_021468890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324565.1
CDS	990..3809
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	XM_021468890.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468890.1

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a4a ( XM_009295486.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293761.1 Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa25974
Clone ID Related Accession (Same CDS sequence)	XM_005161357.3 , XM_009295486.2 , XM_005161357.4 , XM_021468890.1 , XM_009295486.3
Accession Version	XM_009295486.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	solute carrier family 8 (sodium/calcium exchanger), member 4a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295486.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293761.1
CDS	928..3747
Translation	MFHLRLSRSSFSSITPCLSSVLLLIFLSGLTHLSQASGDVSHSGPGNCSGEDSCSEGVVLPIWNPQNPSVGDKVARAIVYFVALIYMFLGMSIIADRFMSSIEVITSQEKEITIKKPNGETTTATVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHKFEAGHLGPSTIVGSAAFNMFIIIALCVYVVPDGEVRKIKHLRVFFVTAAWSIFAYIWLYLILSVFSPGVVEVWEAVLTFLFFPLCVVQAWIADRRLLFYKYVHKRYRTDKNRGIIIETEGDGMFTKMDMEMDGQGANSHHKEALDGMLAGVEEGGGGGEEEEARREMARTLKELKQRHPEKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEANPQEEDPHTIYLEFEPSHYQCFENCGSLKLSVTRHGGDSGCTVKVDYRTEDGTANAGSDYEFAEGTLVFKPGETVKELTVGVIDDDIFEEDEHFYVHLSNPRVVHRAEVSILDPNAVSPGNSIIGSSHVPPKAALGNAHSATVTIYDDDHAGIFTFESNSTRVSESVGIMQVKVHRTSGARGKVAVPYHTTEGTAKAGEDYDEVAGKLEFLNDETMKILEVKIIDDEEYEKNKTFTIHLGEPVLLEIGQKHGDSNDNKPAVGAEEEEVAKMGCPSLGEHTKLEVVIEESYEFKNTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFIVSITLIGVLTAVTGDLASHFGCTVGLKDTVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWHSQGKKFQVPPGSLAFSVTLFTILALVCVVTLLYRRRPSVSGGELGGPRTPKLLTAFLFIVLWLIYIMLASLEAYCHVPGF

Target ORF information:

RefSeq Version	XM_009295486.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio solute carrier family 8 (sodium/calcium exchanger), member 4a (slc8a4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295486.3

ORF Insert Sequence:

ATGTTTCATC TGCGCCTTTC GAGATCCTCG TTTTCCTCCA TCACACCATG CCTTTCTTCA 
GTCCTCCTCC TGATCTTCCT CTCAGGCCTC ACTCATCTTT CTCAGGCTAG CGGTGACGTA 
TCCCACAGCG GCCCGGGAAA CTGTTCAGGA GAGGACAGCT GCTCTGAAGG AGTCGTCCTT 
CCCATCTGGA ACCCCCAAAA CCCCTCGGTG GGCGACAAGG TGGCACGTGC CATCGTCTAC 
TTCGTGGCCC TCATCTACAT GTTTCTGGGC ATGTCCATCA TCGCAGATCG CTTCATGTCA 
TCCATTGAAG TCATTACTTC TCAAGAGAAG GAGATTACAA TCAAGAAACC CAACGGCGAG 
ACCACCACGG CCACTGTTCG CATTTGGAAC GAGACCGTAT CCAACTTGAC CCTCATGGCT 
TTGGGATCAT CAGCTCCCGA AATCCTATTA TCCGTGATTG AAGTCTGCGG ACACAAGTTC 
GAAGCTGGTC ACTTGGGCCC GAGTACAATC GTAGGAAGTG CTGCCTTCAA CATGTTCATC 
ATCATCGCCC TCTGCGTTTA CGTCGTCCCA GATGGTGAAG TCCGCAAAAT CAAGCACCTG 
AGGGTCTTCT TTGTGACGGC AGCCTGGAGT ATCTTCGCCT ACATATGGCT CTACTTGATC 
CTGTCGGTCT TCTCTCCTGG TGTGGTGGAA GTTTGGGAGG CCGTTCTGAC TTTCCTGTTC 
TTTCCGCTCT GCGTGGTTCA GGCTTGGATC GCCGACCGCC GCTTGCTGTT TTACAAATAC 
GTCCACAAAC GCTACCGCAC TGACAAGAAC CGAGGCATAA TCATCGAGAC AGAAGGGGAC 
GGGATGTTCA CCAAGATGGA CATGGAGATG GATGGCCAAG GCGCCAATTC CCACCATAAG 
GAAGCACTAG ATGGGATGCT GGCTGGTGTA GAGGAAGGAG GAGGCGGAGG AGAAGAAGAA 
GAGGCCAGAA GAGAAATGGC TCGAACTCTG AAGGAGCTGA AGCAGAGACA CCCGGAGAAA 
GACATGGAGC AGCTGATTGA GATGGCCAAT TATCAAGTGC TGGTGCAGCA GCAGAAGAGT 
CGAGCTTTCT ATCGCATCCA AGCCACTCGG ATGATGATCG GCGCTGGGAA CATCCTAAAA 
AAGCATGCAG CAGACCAGGC TAGGAAGGTG GTGAGCTGCC ATGAGGCAAA CCCGCAGGAG 
GAGGACCCTC ACACCATCTA CCTGGAGTTT GAGCCCTCAC ATTACCAATG CTTCGAGAAC 
TGCGGCTCCT TGAAGCTCTC TGTGACCCGG CATGGAGGAG ATAGTGGCTG CACCGTCAAG 
GTTGACTATC GCACAGAGGA TGGCACCGCT AACGCCGGAT CCGACTATGA GTTTGCTGAG 
GGCACTCTGG TCTTCAAACC AGGAGAGACG GTTAAGGAGC TCACGGTCGG CGTGATTGAT 
GACGACATAT TCGAGGAAGA TGAGCATTTC TACGTTCACC TCAGCAACCC TCGAGTCGTC 
CACCGTGCCG AAGTCTCCAT CCTCGACCCC AATGCGGTGT CGCCGGGGAA CAGCATCATA 
GGCTCCAGCC ACGTTCCTCC AAAAGCCGCC CTGGGAAATG CTCACTCTGC CACAGTGACC 
ATTTATGACG ATGACCACGC GGGGATCTTC ACCTTCGAGA GCAATTCAAC AAGAGTGAGC 
GAGAGCGTGG GGATCATGCA GGTGAAGGTC CACAGGACAT CTGGAGCGAG GGGCAAGGTG 
GCAGTCCCGT ACCACACTAC TGAGGGCACT GCCAAAGCTG GAGAAGATTA TGACGAGGTG 
GCCGGCAAAC TGGAGTTCCT CAATGATGAG ACCATGAAGA TCCTGGAGGT GAAGATCATT 
GACGATGAGG AGTACGAGAA GAACAAAACC TTCACCATCC ACCTGGGGGA GCCGGTGTTG 
CTGGAGATCG GGCAGAAACA CGGAGACTCT AATGATAACA AACCAGCAGT GGGTGCTGAG 
GAAGAAGAGG TGGCCAAGAT GGGCTGTCCG AGTCTGGGAG AGCACACCAA ACTGGAGGTG 
GTCATAGAAG AGTCGTATGA GTTTAAGAAT ACCGTGGACA AGCTAATAAA GAAGACTAAC 
CTTGCGCTGG TGGTGGGCAG CAGTAGCTGG AGAGAGCAGT TTGTCAGCGC CGTCACTGTC 
AGTGCAGGAG ATGACGATGA GGAAGAGAGC GGAGAAGAGC GCTTGCCCTC CTGCTTCGAT 
TACATCATGC ACTTTCTGAC GGTTTTCTGG AAGGTTCTGT TCGCTTTCGT ACCCCCAACA 
GAATACTGGA ATGGCTGGGC CTGCTTTATT GTCTCCATCA CACTCATCGG GGTCCTGACT 
GCTGTAACTG GAGATCTGGC ATCTCATTTT GGTTGTACAG TAGGCCTGAA GGACACAGTC 
ACAGCCGTGG TGTTTGTGGC CTTGGGCACT TCTGTACCTG ACACCTTTGC CAGCAAGGTG 
GCAGCCATCC AGGACCAATA CGCCGATGCG TCCATTGGAA ATGTGACCGG TAGTAATGCA 
GTTAATGTCT TCCTGGGTAT CGGAGTGGCG TGGACCATCG CTGCAGTGTA CTGGCACAGT 
CAAGGCAAAA AATTCCAAGT CCCACCAGGG TCATTGGCGT TCTCCGTCAC CCTCTTCACC 
ATCCTGGCAC TGGTATGTGT TGTGACACTA CTGTACCGCC GCCGGCCCTC TGTGTCCGGG 
GGCGAGTTGG GGGGCCCGCG AACCCCCAAG CTGCTCACCG CATTCCTCTT TATCGTGCTG 
TGGCTCATCT ACATCATGCT GGCCTCGCTG GAGGCCTACT GCCACGTGCC TGGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The ?2Na+/K+-ATPase is critical for skeletal and heart muscle function in zebrafish.
Journal of cell science125(Pt 24)6166-75(2012 Dec)
Doganli C,Kjaer-Sorensen K,Knoeckel C,Beck HC,Nyengaard JR,Honor? B,Nissen P,Ribera A,Oxvig C,Lykke-Hartmann K

Effects of dietary methylmercury on the zebrafish brain: histological, mitochondrial, and gene transcription analyses.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine25(1)165-80(2012 Feb)
Cambier S,Gonzalez P,Mesmer-Dudons N,Br?thes D,Fujimura M,Bourdineaud JP

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slc8a4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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