nckap5l cDNA ORF clone, Danio rerio(zebrafish)

The following nckap5l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nckap5l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa27770 XM_021473691.1
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27770 XM_005162212.4
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27770 XM_005162213.4
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27770 XM_021473692.1
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27771 XM_021473693.1
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27771 XM_021469964.1
Latest version!
Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27771 XM_017353711.1
Latest version!
Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa27770 XM_005162213.3 Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa27770 XM_005162212.3 Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_005162213.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162213.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162270.1
    CDS467..4912
    Translation

    Target ORF information:

    RefSeq Version XM_005162213.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162213.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_005162213.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162270.1
    CDS517..4962
    Translation

    Target ORF information:

    RefSeq Version XM_005162213.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_005162212.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162269.1
    CDS234..4679
    Translation

    Target ORF information:

    RefSeq Version XM_005162212.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_005162212.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162212.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005162269.1
    CDS228..4673
    Translation

    Target ORF information:

    RefSeq Version XM_005162212.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005162212.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_021473691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395151.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329366.1
    CDS228..4673
    Translation

    Target ORF information:

    RefSeq Version XM_021473691.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27770
    Clone ID Related Accession (Same CDS sequence) XM_005162213.4 , XM_005162213.3 , XM_005162212.3 , XM_005162212.4 , XM_021473691.1 , XM_021473692.1
    Accession Version XM_021473692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395151.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329367.1
    CDS467..4912
    Translation

    Target ORF information:

    RefSeq Version XM_021473692.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCA GCCAGGACCC
    TGTCTGGAGC ACATTGGTGA GCTACAGCAG AGTGAATCTA CCAAACTGAT TGAGGCTCAA
    AGGAATGCAC AGGCCAAGGG TAACAGCGAA TGTGCACAAA ATTCCGTACC CGAGACTCAA
    GGCTCGTCCA CATCCATGGA GGCCATGTCC CCATTCTTGA AGAAGAAAGC ACACATTTTG
    GAGGTTCTCC GCAAACTTGA AGAAACAGAT CCACTTCGGT ACCATCGTTC CTCTGGCATT
    CCCTCCATCT GTGACTACAG TCAGGCTCTT GCCTCTAAGG AGGCTGTATT AGCCTGCAGA
    GACAATACAT CCAGGTGCAA TGCACCCAAA GCACATTGTT CCCATTCCTG TCCAGATGTT
    TGCACACATC AGCATTTGAA TGGAGGAGAG CACAGCAATG TAAAGTGTGG TAGTTGTAGC
    ACATGTTTGA TGCTGTCCAA TAAAGATTCC GTCAGCTTAG CCAAGAGTAG CCACAACTGT
    TGCACTTCAA CCCCCATTAC CATTGATAAC CTCAATTTGG CTCGGCCCGA TTTCCAAGAG
    AATGTGGAAA TGCAGAGCCT TGTGAAACCT GCCACAGGTA CAAGTGAACT GTTTGTAAAC
    AGGACTTCTG GAGATATGTC GATTCTTTCA GCAGAGCAAG GTCTTATACC GTCAGTTGAT
    CCTCACTCAT TAGAAGCCCC TGAATCATAC TCAAGATTGA CTATTTCAGA AAGAGATCAG
    CCAAGGGGAG TCTCTGACCA TGTGTTGGTA GATACAAGAG AAAAGAATAG GTTGTCTGAT
    CTTCTCCTTG AGTGTCAATC CAACAGGTCA AGCAGCAGCA CCTGTAATGA GACTATTTGT
    GACGATGGCA CAATTGATGT GGTGCTGCTA GACTCAGATT TAAAGCACTT AAATGCTATA
    GCACCTCAAA GGCTTGATAA AGAAGAGTTT GCGACAAAGG ATGACTCTGA CTGTGTCTAT
    CGAGCCAACA TAGCCTCCTC AAAGCTGCCT ACTCTTGGAC TAAAAGACAC ACACTGTGAG
    ATTGACAGCA GCGAACAGGT CCACAAAGGT CTGTTATTTT CTCCCAATGA GAATCATACT
    GCCTCAAAAG TACTGGATGA CATCTCTGTA CCTGTAAAAG ATACCCCATC TGACCCTCAG
    TCCATTAGAT CGAAAGTTGC AGTCAGCCCG AGTCAAGTTT CCTGCCTTAG AGAGGTCAAG
    GCCTCACCCT CCAAACTGCT CAAGTTCCTT AAGATCCCCA CTATTGGAGA ACGTACTGCA
    GTACCTCCAC CTCGTCTCAG TCCTCAGTTG ACAAGAAGCT CAAAGATTCC TTGCCGGAGC
    AACAACTATG AGGTGCACAG TTCACCTGTA GCCACACGAA AAGCCACCAC GACTGAGAGG
    CAAAGACAGG TGACCCCTCC AAAATCAGAG TCACACTCTG CTCCAACTTC TCCACCCCAA
    CCTGATGATG TCCCCACACC TTTGCCAAAG GATCTTGGTT GTGGTATCCC TAAAACAAGT
    CGTGTAGCTA AGCCTACACA AAGTACTCAC AATCAGAAAA CCCCTCAGAA AATTCCACAC
    TACGAAAATG TCTCAGATAT CTCCAAAACG AGCAACACTG ATGTATCCCA GCTGCTTGGA
    GAAGTGAGCT CATCTCTTCA AAATTCCAGC CCTGCTAATG AAGGTGCAAA AGGAAAGCAG
    ACAAAGTTTG GTGTAGAGAG ACCAACGAGT CTTAAGCATA AGGAGATCTC ACCAGAGTCA
    GATTCTTCAG ACCAAGAGGA CAGCTCGGAG AGCCCAGTGT GGCATAAGCA AGTCAATCAT
    CGCAGTCTGC CCAGTCAGTC ATCATCCCAG AAGGCTCAGA CTGGGATTAG CACTAGATCA
    AGGAGCCAAG AGAAAAATGA AATGGCAGAC AATGATTCAC AGAGCTCAGA ATCACCTTTT
    AAAAGGAGCG ATCCACCGCC TGTTCCTAAG AAATCAGGGA TTGGAAGACA TTCAGGTGAG
    TCAAGTCATT CCTTTAAAGA ACGACTGGCT GCACTTGGCA AGTTGAGGAA CCCAGATGAG
    TCTGCAGCAT GTCCTCCACT TGCAGAGAAG AAAGAAATCC AGTCTGCTGC AAGCATACCA
    ACAAGAACCA CAGATAAAAG CAAAACTGCT GACAGAAACT CTGATTGCAA GTCAGGGGAG
    TATAGACTTC CTAAAAACAC AGATTCAATT GAGGACAAAT CATACCATTC AGGTCATTAT
    GATGGTGCTG TTTCAAAACA ACCGGGAGCC TTCCAGCATG TTGAGCAAAC TGTCAGGTCT
    CTTCCGTCCA ACACGTTAAT CTCCAAACTT GAACACGAAT CCCCTAGAAC ATTCATAGCC
    AAGCAAGAGA GCCCGAAATC TAAGATGCAC CAACCGTCTG CTAACCTAGA AGCAGCACAG
    GCTTTGCGTA GCTATGCTAA GAGTACAACT CCCCATAGCC TTTCATATAA CGGAAAAAAT
    GTTGCTAGTC CCCATAGTAG CAGCCCCAAT AAAATCCCTC TCAAGTCACC AACTAAAACT
    GTGCCACCCT CTATGAACCG AGAAGGGAAA CCTACTCCAG AGTTTCAAAG GTACATGTCC
    AAATCTGAAG ACAGGAGCCA TCTCAGGGCC AATAAAAAGA AGAATTCTTC CTATGGAGAG
    AGTCTTCCTC CGCCGCCACC AAGGCCGGTA GAGGCCGTTG AAGAAAAGAG GCACTCGGCC
    CCCAGTCCTC AGTCTTCTAT TGAGCAAAAG GTCATGAAGG GCATTGAGGA AAATGTGCTG
    AAGCTGCAAG AGCAAGACAA AGGTCAGGTG GTCGAGGTCA AGCAAAAGGC TTCCAATGGA
    ATCGCCAACT GGTTTGGTCT CAGAAAAAGC AAGCTTCCAG CTCTTAGCCG TAAACCAGAA
    ACCTCGAAAG TGAAAGATGA CAAGAGAGAA TGGAAACTCA ATATATCCAA GGATTCCAAG
    GGGGCTGCGA AGAAACCTGA GAGTGAGAGC CTAAACATTT CAATGCTGAT TGAGAAGGCA
    GAGGGCTTGC ATAAAGCTCT TGAAGAAGAA CGGGCTTATG TGAATGGAGT GGCGTTGGAC
    CGTCAAGGAA AGGGACATTC ATGTGAGGTA GTGATGGACC AAGCCCAAGG TCAGCTGTCT
    GTGATGTATA GAGGAGTGCC ATCTGACAAC TTCATGCAGC AACTTCTGAA CCGGGTTGAT
    GGGAAGGATA CTAGTGGCAT CAGTATGGCA CATAGACGTC TCTCATTTGA CTGTAAATCC
    AGACCTGTTT TTAGTCACCA GAGTGCTGCC ATTGCTAGTC AGACACGAAG CAGTGATGAC
    ATGGAAAAGG TCTCTGCATT ACTAAGTAAA GACGTCACGT CGGACGACAA CCTGGCTGAG
    ACAATTCATC ATGAACACTT TGCAGGATCT GGAATCTCCA CACACACTCT TGACAGCGGC
    ATCGGCACCT TCCCTCTTCC AGACTACAGT GCAAACGCTG GTTGTAAAAG CATCCCAAAA
    GGAAAGGCCC ACGGGGAGAG TGACCCTTCT CATCTCCAGC CCAAACACGC ATCAGGGATG
    AAGATGTCCC ACAAAGCCTG GAGCCTGGAG AGAGAGTTGT CATCATTAGA GGAGGATTAT
    ATGGGCGGTC AGGACATGGA CCACCACACC AGCACTATAG AAAACAAGAT TCCCTCCAGC
    CAGATTGCAA ACATAGTTCA TGATGGCACT GATATCTGTG GAGCCCCTAT GAAATTAGGC
    CCCACAAAGA ACTGGACTTT CCCAAACCTC AAAGCATCTG CGGAGTCAAC TGAGGTTTAT
    CTGGGTGTTG GAGACGCAGT GGAGCCGGTG ACTCACAAGA GTTCATTTAA GAGGCGCAAC
    AAGACTAGTG ATTCAGTGGC CAGTCGTGAG GGAGATCCAA GAAGTCTTCC TCAGCCCAGT
    CAGACACGCA GAGTCAAGAG CCGCATTCCT GGAAACCCTG AGCTGGTGCG GGACACTGGT
    CTGGAGCTGG TAAGAGAGCG ACCCGACGAC GGCCTGTCCC CAAACCACCC GCAGGTCCTA
    GAGACGCCCG AGTCTCTGAG CGATTCCCTC TACGACAGCC TGTCCTCGTG TGGCAGTCAA
    GGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27771
    Clone ID Related Accession (Same CDS sequence) XM_021469964.1 , XM_017353711.1 , XM_021473693.1
    Accession Version XM_021469964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325639.1
    CDS228..4670
    Translation

    Target ORF information:

    RefSeq Version XM_021469964.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27771
    Clone ID Related Accession (Same CDS sequence) XM_021469964.1 , XM_017353711.1 , XM_021473693.1
    Accession Version XM_017353711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nck-associated protein 5-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209200.1
    CDS518..4960
    Translation

    Target ORF information:

    RefSeq Version XM_017353711.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio wu:fb77e12 (wu:fb77e12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa27771
    Clone ID Related Accession (Same CDS sequence) XM_021469964.1 , XM_017353711.1 , XM_021473693.1
    Accession Version XM_021473693.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4443bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nck-associated protein 5-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395151.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329368.1
    CDS228..4670
    Translation

    Target ORF information:

    RefSeq Version XM_021473693.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCGGAAG AGGCTCAAAA TAGAGTAGAT GAGGACTTTG AGTCTGAGGA GGGTGATTTG 
    GAGTCCTATC TAGAGGAAGA GAGTAGCAGT GAGCTTCTGG AGCGAGTGAG AGAACTTCAA
    GCAGAGAACT CTGCCCTTTC ATTAGCAAAC GAGAGTCAGA GAGAAGCATA TGAACGATGC
    TTGGATGAGG TGGCCAACCA TGTTGTCCAG GCTTTACTCA ATCAAAAGGA TCTCAGAGAG
    GAATGCATCA AACTGAAAAT GCGTGTTTTT GATTTGGAGA GGCAGAACAG AACACTGTGT
    GAGCTTCTGC AGGAGAAACT GCACAGTCAG TCTTGTGTAC ATCAGCAGCC AGGACCCTGT
    CTGGAGCACA TTGGTGAGCT ACAGCAGAGT GAATCTACCA AACTGATTGA GGCTCAAAGG
    AATGCACAGG CCAAGGGTAA CAGCGAATGT GCACAAAATT CCGTACCCGA GACTCAAGGC
    TCGTCCACAT CCATGGAGGC CATGTCCCCA TTCTTGAAGA AGAAAGCACA CATTTTGGAG
    GTTCTCCGCA AACTTGAAGA AACAGATCCA CTTCGGTACC ATCGTTCCTC TGGCATTCCC
    TCCATCTGTG ACTACAGTCA GGCTCTTGCC TCTAAGGAGG CTGTATTAGC CTGCAGAGAC
    AATACATCCA GGTGCAATGC ACCCAAAGCA CATTGTTCCC ATTCCTGTCC AGATGTTTGC
    ACACATCAGC ATTTGAATGG AGGAGAGCAC AGCAATGTAA AGTGTGGTAG TTGTAGCACA
    TGTTTGATGC TGTCCAATAA AGATTCCGTC AGCTTAGCCA AGAGTAGCCA CAACTGTTGC
    ACTTCAACCC CCATTACCAT TGATAACCTC AATTTGGCTC GGCCCGATTT CCAAGAGAAT
    GTGGAAATGC AGAGCCTTGT GAAACCTGCC ACAGGTACAA GTGAACTGTT TGTAAACAGG
    ACTTCTGGAG ATATGTCGAT TCTTTCAGCA GAGCAAGGTC TTATACCGTC AGTTGATCCT
    CACTCATTAG AAGCCCCTGA ATCATACTCA AGATTGACTA TTTCAGAAAG AGATCAGCCA
    AGGGGAGTCT CTGACCATGT GTTGGTAGAT ACAAGAGAAA AGAATAGGTT GTCTGATCTT
    CTCCTTGAGT GTCAATCCAA CAGGTCAAGC AGCAGCACCT GTAATGAGAC TATTTGTGAC
    GATGGCACAA TTGATGTGGT GCTGCTAGAC TCAGATTTAA AGCACTTAAA TGCTATAGCA
    CCTCAAAGGC TTGATAAAGA AGAGTTTGCG ACAAAGGATG ACTCTGACTG TGTCTATCGA
    GCCAACATAG CCTCCTCAAA GCTGCCTACT CTTGGACTAA AAGACACACA CTGTGAGATT
    GACAGCAGCG AACAGGTCCA CAAAGGTCTG TTATTTTCTC CCAATGAGAA TCATACTGCC
    TCAAAAGTAC TGGATGACAT CTCTGTACCT GTAAAAGATA CCCCATCTGA CCCTCAGTCC
    ATTAGATCGA AAGTTGCAGT CAGCCCGAGT CAAGTTTCCT GCCTTAGAGA GGTCAAGGCC
    TCACCCTCCA AACTGCTCAA GTTCCTTAAG ATCCCCACTA TTGGAGAACG TACTGCAGTA
    CCTCCACCTC GTCTCAGTCC TCAGTTGACA AGAAGCTCAA AGATTCCTTG CCGGAGCAAC
    AACTATGAGG TGCACAGTTC ACCTGTAGCC ACACGAAAAG CCACCACGAC TGAGAGGCAA
    AGACAGGTGA CCCCTCCAAA ATCAGAGTCA CACTCTGCTC CAACTTCTCC ACCCCAACCT
    GATGATGTCC CCACACCTTT GCCAAAGGAT CTTGGTTGTG GTATCCCTAA AACAAGTCGT
    GTAGCTAAGC CTACACAAAG TACTCACAAT CAGAAAACCC CTCAGAAAAT TCCACACTAC
    GAAAATGTCT CAGATATCTC CAAAACGAGC AACACTGATG TATCCCAGCT GCTTGGAGAA
    GTGAGCTCAT CTCTTCAAAA TTCCAGCCCT GCTAATGAAG GTGCAAAAGG AAAGCAGACA
    AAGTTTGGTG TAGAGAGACC AACGAGTCTT AAGCATAAGG AGATCTCACC AGAGTCAGAT
    TCTTCAGACC AAGAGGACAG CTCGGAGAGC CCAGTGTGGC ATAAGCAAGT CAATCATCGC
    AGTCTGCCCA GTCAGTCATC ATCCCAGAAG GCTCAGACTG GGATTAGCAC TAGATCAAGG
    AGCCAAGAGA AAAATGAAAT GGCAGACAAT GATTCACAGA GCTCAGAATC ACCTTTTAAA
    AGGAGCGATC CACCGCCTGT TCCTAAGAAA TCAGGGATTG GAAGACATTC AGGTGAGTCA
    AGTCATTCCT TTAAAGAACG ACTGGCTGCA CTTGGCAAGT TGAGGAACCC AGATGAGTCT
    GCAGCATGTC CTCCACTTGC AGAGAAGAAA GAAATCCAGT CTGCTGCAAG CATACCAACA
    AGAACCACAG ATAAAAGCAA AACTGCTGAC AGAAACTCTG ATTGCAAGTC AGGGGAGTAT
    AGACTTCCTA AAAACACAGA TTCAATTGAG GACAAATCAT ACCATTCAGG TCATTATGAT
    GGTGCTGTTT CAAAACAACC GGGAGCCTTC CAGCATGTTG AGCAAACTGT CAGGTCTCTT
    CCGTCCAACA CGTTAATCTC CAAACTTGAA CACGAATCCC CTAGAACATT CATAGCCAAG
    CAAGAGAGCC CGAAATCTAA GATGCACCAA CCGTCTGCTA ACCTAGAAGC AGCACAGGCT
    TTGCGTAGCT ATGCTAAGAG TACAACTCCC CATAGCCTTT CATATAACGG AAAAAATGTT
    GCTAGTCCCC ATAGTAGCAG CCCCAATAAA ATCCCTCTCA AGTCACCAAC TAAAACTGTG
    CCACCCTCTA TGAACCGAGA AGGGAAACCT ACTCCAGAGT TTCAAAGGTA CATGTCCAAA
    TCTGAAGACA GGAGCCATCT CAGGGCCAAT AAAAAGAAGA ATTCTTCCTA TGGAGAGAGT
    CTTCCTCCGC CGCCACCAAG GCCGGTAGAG GCCGTTGAAG AAAAGAGGCA CTCGGCCCCC
    AGTCCTCAGT CTTCTATTGA GCAAAAGGTC ATGAAGGGCA TTGAGGAAAA TGTGCTGAAG
    CTGCAAGAGC AAGACAAAGG TCAGGTGGTC GAGGTCAAGC AAAAGGCTTC CAATGGAATC
    GCCAACTGGT TTGGTCTCAG AAAAAGCAAG CTTCCAGCTC TTAGCCGTAA ACCAGAAACC
    TCGAAAGTGA AAGATGACAA GAGAGAATGG AAACTCAATA TATCCAAGGA TTCCAAGGGG
    GCTGCGAAGA AACCTGAGAG TGAGAGCCTA AACATTTCAA TGCTGATTGA GAAGGCAGAG
    GGCTTGCATA AAGCTCTTGA AGAAGAACGG GCTTATGTGA ATGGAGTGGC GTTGGACCGT
    CAAGGAAAGG GACATTCATG TGAGGTAGTG ATGGACCAAG CCCAAGGTCA GCTGTCTGTG
    ATGTATAGAG GAGTGCCATC TGACAACTTC ATGCAGCAAC TTCTGAACCG GGTTGATGGG
    AAGGATACTA GTGGCATCAG TATGGCACAT AGACGTCTCT CATTTGACTG TAAATCCAGA
    CCTGTTTTTA GTCACCAGAG TGCTGCCATT GCTAGTCAGA CACGAAGCAG TGATGACATG
    GAAAAGGTCT CTGCATTACT AAGTAAAGAC GTCACGTCGG ACGACAACCT GGCTGAGACA
    ATTCATCATG AACACTTTGC AGGATCTGGA ATCTCCACAC ACACTCTTGA CAGCGGCATC
    GGCACCTTCC CTCTTCCAGA CTACAGTGCA AACGCTGGTT GTAAAAGCAT CCCAAAAGGA
    AAGGCCCACG GGGAGAGTGA CCCTTCTCAT CTCCAGCCCA AACACGCATC AGGGATGAAG
    ATGTCCCACA AAGCCTGGAG CCTGGAGAGA GAGTTGTCAT CATTAGAGGA GGATTATATG
    GGCGGTCAGG ACATGGACCA CCACACCAGC ACTATAGAAA ACAAGATTCC CTCCAGCCAG
    ATTGCAAACA TAGTTCATGA TGGCACTGAT ATCTGTGGAG CCCCTATGAA ATTAGGCCCC
    ACAAAGAACT GGACTTTCCC AAACCTCAAA GCATCTGCGG AGTCAACTGA GGTTTATCTG
    GGTGTTGGAG ACGCAGTGGA GCCGGTGACT CACAAGAGTT CATTTAAGAG GCGCAACAAG
    ACTAGTGATT CAGTGGCCAG TCGTGAGGGA GATCCAAGAA GTCTTCCTCA GCCCAGTCAG
    ACACGCAGAG TCAAGAGCCG CATTCCTGGA AACCCTGAGC TGGTGCGGGA CACTGGTCTG
    GAGCTGGTAA GAGAGCGACC CGACGACGGC CTGTCCCCAA ACCACCCGCA GGTCCTAGAG
    ACGCCCGAGT CTCTGAGCGA TTCCCTCTAC GACAGCCTGT CCTCGTGTGG CAGTCAAGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.