plekha5 cDNA ORF clone, Danio rerio(zebrafish)

The following plekha5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plekha5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa09067 NM_001327786.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa09061 XM_005164474.4
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa09066 XM_005164479.4
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142020 XM_021475077.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142021 XM_021475078.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142022 XM_021475079.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142023 XM_021475080.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142024 XM_021475081.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142025 XM_021475082.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142026 XM_021475083.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142027 XM_021475084.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa142028 XM_021475085.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142029 XM_021475086.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142030 XM_021475087.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142031 XM_021475088.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142032 XM_021475089.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142033 XM_021475090.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142034 XM_021475091.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa142035 XM_021475093.1
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45107 XM_009299998.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45108 XM_009299999.3
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45109 XM_009300000.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45110 XM_009300001.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45111 XM_009300002.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45112 XM_009300003.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45113 XM_009300004.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45114 XM_009300005.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45115 XM_009300006.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45116 XM_009300008.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45117 XM_009300009.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45118 XM_009300010.3
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45119 XM_009300011.3
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45120 XM_009300012.2
Latest version!
Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
ODa45118 XM_009300010.2 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa09066 XM_005164479.3 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa09061 XM_005164474.3 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45119 XM_009300011.2 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45112 XM_009300003.1 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ODa45108 XM_009299999.2 Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa09067
    Clone ID Related Accession (Same CDS sequence) NM_001327786.1
    Accession Version NM_001327786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LO018342.1, BX088564.10 and BX914207.15. On May 25, 2016 this sequence version replaced XM_690591.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC155854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT
    CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001314715.1
    CDS21..3737
    Translation

    Target ORF information:

    RefSeq Version NM_001327786.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001327786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT
    CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09061
    Clone ID Related Accession (Same CDS sequence) XM_005164474.3 , XM_005164474.4
    Accession Version XM_005164474.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC
    TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT
    CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164531.1
    CDS44..4201
    Translation

    Target ORF information:

    RefSeq Version XM_005164474.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164474.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC
    TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT
    CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09061
    Clone ID Related Accession (Same CDS sequence) XM_005164474.3 , XM_005164474.4
    Accession Version XM_005164474.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164474.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC
    TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT
    CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164531.1
    CDS42..4199
    Translation

    Target ORF information:

    RefSeq Version XM_005164474.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164474.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGCATCATG TGACAGGAAG AACACATCAT CACCATCGTC ATCATCAGAC
    TGGAAGTCTC GCCCCCTCCT TCATCAGCTG CCCAACACAA ACACAACAGC GTCCAGGCCT
    CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09066
    Clone ID Related Accession (Same CDS sequence) XM_005164479.4 , XM_005164479.3
    Accession Version XM_005164479.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164479.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
    ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC
    ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC
    ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC
    TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC
    TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA
    AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA
    GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG
    CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA
    CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC
    AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG
    CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA
    GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC
    GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA
    TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG
    CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG
    CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA
    ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC
    TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT
    GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA
    GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC
    CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG
    AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG
    CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA
    GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG
    CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT
    CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG
    GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG
    TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC
    AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG
    TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG
    CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC
    GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC
    TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG
    AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164536.1
    CDS329..4123
    Translation

    Target ORF information:

    RefSeq Version XM_005164479.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164479.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
    ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC
    ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC
    ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC
    TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC
    TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA
    AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA
    GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG
    CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA
    CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC
    AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG
    CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA
    GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC
    GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA
    TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG
    CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG
    CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA
    ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC
    TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT
    GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA
    GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC
    CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG
    AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG
    CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA
    GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG
    CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT
    CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG
    GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG
    TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC
    AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG
    TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG
    CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC
    GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC
    TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG
    AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09066
    Clone ID Related Accession (Same CDS sequence) XM_005164479.4 , XM_005164479.3
    Accession Version XM_005164479.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
    ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC
    ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC
    ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC
    TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC
    TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA
    AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA
    GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG
    CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA
    CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC
    AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG
    CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA
    GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC
    GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA
    TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG
    CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG
    CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA
    ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC
    TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT
    GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA
    GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC
    CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG
    AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG
    CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA
    GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG
    CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT
    CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG
    GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG
    TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC
    AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG
    TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG
    CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC
    GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC
    TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG
    AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164536.1
    CDS332..4126
    Translation

    Target ORF information:

    RefSeq Version XM_005164479.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164479.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGAAG CCTCAAACTA CACCGGCGGC TCCGACTACA CCACACATCC CAGCAGCCCC 
    ACAACCAGAC CATCCCGGTC CTCAAAGAAA GTGCACAACT TTGGGAAAAG GTCCAACTCC
    ATCAAGAGGA ACCCCAATGC ACCTGTGATC AAAAACGGCT GGCTTCACAA ACAGGACAGC
    ACTGGGATGA AGATGTGGAA GAAGAGATGG TTCGTTCTGT CTGATATGTG CCTCTTCTAC
    TACAGAGATG AGAAGGAAGA GGGGATTCTG GGAAGCATCC TCCTGCCTAG CTTTCACATC
    TCCATGCTGT CCGTGGACGA CCATATTACC CGGAAATACG CCTTCAAGGC GACGCATCCA
    AACATGCGGA CGTATTATTT CAGCACAGAC ACAGCCAAAG ATATGGAGTC CTGGATGAAA
    GTGATGTCGG ATGCGGCGAT GGCTCACAGC GAGCCAATCA GAAGGCTTGA GAAGGTAAAG
    CTGGACTCTC GCGTTCCTCA GGAGATGAAC AACATGCTGA ACCACCGCGT CTTGACACGA
    CCCGAGATCC AAAACAACGA GAGGAACCGC GAGACGCTCC GCCAAGAGGA CAAGAAGAGC
    AAACCATCAG AGAAACCTCA GAAAGACAAA GACAGAGTCA GCATGCAGAA AGACGGAGAG
    CGCTTCAGTG AAAACTACAC TCACCGGCAG AAAGACGGCG AGCGCTACAT GCTGCAGAAA
    GACCTGGAGA AATTTGTCCT GCAGAAAGAC GGAGAGAAGT ACGCCGTGCA GAAAGAAGGC
    GACAAATATC TCCTGCAGAA AGACGGACAC AAATACACAG TGCACAAAGA CGGAGACAAA
    TACACGCTTC AGAGAGAACT CGAGATGTAC AATCCACCAA AAGACGCGGA GAAGATCCCG
    CAGAAAGATG CAAGGTTTAG TCTGGTGCAG AAGGAGCTGG AGAAACCCGT CCCGCCACCG
    CGATCTGAGG AAAAATACGG CTTTCAGAAA GAGGGCACCA TTGAGCGTCC GCTAACTAAA
    ATAAATAGCA TTAAACTGCA GCCGGCTCAA GCAGCCGCCA TCGCTGCAGC CGTTTCATCC
    TCTAGGATGA TGCAGAAACA GGTTAATGGC TCCGGGGAAC GAGCGGGAGA CAGAAGTCCT
    GGAGATTTGA GTACTGTGAG GACGAGCGTC CCGCCACCAA CGCAGCCGGC TCCAGAACCA
    GAGCGAAGCC TCAGCAGAAC CAGCTCAATG CAGCAGCTGG AGCAGTGGGT TCGCACACAC
    CGCACACGAG CTCCAGACGA CGACACCAGG AGCATTACGT CCTACCAGAC CCTGCCAAGG
    AACATGCCGA GCCACCGGGC GCAGATTGTC CCTCGCTACC CTGAGGGCTA CCGCACTCTG
    CCCCGAAACA TGCTGCGTCC GGACAGCATC TGCAGTGTAG CAGGATCAGT GTACGACCGA
    GCGCTTCAGC CCAGCACGCA GGAAAAGCGG CGCTCCATCC GAGATGACAC CATGTGGCAG
    CTGTACGAGT GGCAGCAGCG GCAGGCGCAC AGTCGGCTTG GGTACGGGAC GCTGCCCAGT
    CCCAAAACTA TGGGGCAGAT CGCGGAGTCC ATCCCCACGT CCCCATCCCA TGGCTCTCTG
    GCCGGATATC ACACCTTCTC CCCGAACAGA CCGCTCAACC CTGACTCGCG CTCGGAGCTG
    TCTTCGCCTG TGTTTCGTGG AGACGTGACC ATCGAGCGAC GCCACCGACC ACACCTGTCC
    AAGTACTCCT ATCCTGCGGA ACGCAGAGCT GCTCCTCCCG CACAGAACAT CACTGCACAG
    TCTCTTCAAG GCAAAACGCC AGAGGAACTG ACCCTGCTGC TCATCAAACT GCGGCGGCAG
    CAGGCCGAGC TCAACAGCCT CCGTGAGCAC ACACTCGCGC AGCTCATGCA GCTGAACCTC
    GACGCCACCA ACCCAAAGAG TGAAATCCTC TCTCATCACC TGCAGAGGAA TCTCATGTAC
    TTGGACAGTC AGATGAAGGA GAACGAACCC ATAATCTTCA TGATTCACAC TATGATTGAG
    AACTCGGCGC CCCGGCCGCA GCTCTACCAG CAACAGATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142020
    Clone ID Related Accession (Same CDS sequence) XM_021475077.1
    Accession Version XM_021475077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330752.1
    CDS42..4238
    Translation

    Target ORF information:

    RefSeq Version XM_021475077.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142021
    Clone ID Related Accession (Same CDS sequence) XM_021475078.1
    Accession Version XM_021475078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC
    AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT
    CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330753.1
    CDS42..4235
    Translation

    Target ORF information:

    RefSeq Version XM_021475078.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC
    AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT
    CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142022
    Clone ID Related Accession (Same CDS sequence) XM_021475079.1
    Accession Version XM_021475079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGTGAACAGT
    GTGTCGACTC CTCCTCAATC TCCAAGCTCC TCCTCCTCTC CTCCGCCTCC ACCCTCCCCT
    CCACAGCTGA CGGACGGGTC ACACTTCATG TGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330754.1
    CDS42..4220
    Translation

    Target ORF information:

    RefSeq Version XM_021475079.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGTGAACAGT
    GTGTCGACTC CTCCTCAATC TCCAAGCTCC TCCTCCTCTC CTCCGCCTCC ACCCTCCCCT
    CCACAGCTGA CGGACGGGTC ACACTTCATG TGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142023
    Clone ID Related Accession (Same CDS sequence) XM_021475080.1
    Accession Version XM_021475080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG
    AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC
    GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG
    CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG
    ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC
    CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG
    CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG
    CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT
    CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA
    CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA
    AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT
    GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA
    ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG
    GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT
    AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT
    ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC
    ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG
    CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA
    ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG
    AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC
    ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC
    GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA
    GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG
    GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT
    CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG
    GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA
    GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC
    AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA
    CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC
    ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330755.1
    CDS42..4157
    Translation

    Target ORF information:

    RefSeq Version XM_021475080.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG
    AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC
    GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG
    CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG
    ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC
    CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG
    CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG
    CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT
    CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA
    CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA
    AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT
    GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA
    ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG
    GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT
    AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT
    ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC
    ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG
    CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA
    ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG
    AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC
    ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC
    GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA
    GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG
    GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT
    CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG
    GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA
    GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC
    AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA
    CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC
    ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142024
    Clone ID Related Accession (Same CDS sequence) XM_021475081.1
    Accession Version XM_021475081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA
    GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA
    CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT
    TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC
    GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA
    GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC
    ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG
    CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA
    CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA
    GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA
    TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG
    CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG
    GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC
    AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC
    AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC
    ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG
    GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC
    AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA
    TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA
    ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330756.1
    CDS42..4154
    Translation

    Target ORF information:

    RefSeq Version XM_021475081.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA
    GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA
    CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT
    TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC
    GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA
    GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC
    ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG
    CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA
    CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA
    GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA
    TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG
    CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG
    GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC
    AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC
    AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC
    ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG
    GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC
    AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA
    TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA
    ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142025
    Clone ID Related Accession (Same CDS sequence) XM_021475082.1
    Accession Version XM_021475082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330757.1
    CDS42..4118
    Translation

    Target ORF information:

    RefSeq Version XM_021475082.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142026
    Clone ID Related Accession (Same CDS sequence) XM_021475083.1
    Accession Version XM_021475083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGGC CCGGATTACA GGCTGTATAA GAGTGAGCCG
    GAGCTGACCA CAGTAGCCGA AGTCGACGAG AGTAACGGTG AAGACAAATC AGAGCAGACA
    GCAGAGAAAG AGCCGCCCGG CGTCACTAAA GGTATAGTTT ACCCGGTGGG AGTGGTCAGT
    CCCAGAACTA AATCACCCAT GCCCGAGTCC TCCACCATCG CCTCCTACGT CACCTTGAGG
    AAGACCAAAA AACCTGATCC CAGAACATGT CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG
    GAGCAGATGA GCAGTGGTGC TGAAGTGGGC CGAACACGCA TGAGTGTGGA GGAGCAGATG
    GAGAGAATCA GACGACACCA GCAGGGGGCG CTGAGAGAGC GCAGGAGAGA AGATGGCTCT
    CTATCCCGCA GCCTGTCCTT CACTAAGGAC AGCACATACT ACACACTGCA GGCACGTAAG
    AAGAGTCCGG TGATCTCTCC AGACGAGCAG GACGAGCGCA GAGACATGAC GCCTGTGGAG
    CGAGACACAA AACACACAAA ACACCCTGAC GCAGAGGAAA ACTGCAGAGA CACTAAGGAG
    CAGCTGCCAG CACACACCCA CCTGGAGATT AAGGACAAAG CATCTGTGAC TCGCTCGGTC
    TCCATGAAAG AGTCTCTCCT GAAGAGTGTG AGTCCCAAAC CCGTGCTGAA GCAGGACGAA
    TCTGAGTGTA AACTGGAGTA CGACAGAAAG CTGGCCACTC AGAACAGCCT GCAGACGGCA
    GTGATGGTGC GTGTGGGCAC TGAGGAGGAT GAAGAGGATG AAGAAGAGGA GCAGAAAGAC
    TCAGTTCAGC AGCAGGAGGA CTCTTATGAG CTCAGCAACA GCAAACACAG CACACTGATC
    TCAGCATCAT GTGACAGGAA GAACACATCA TCACCATCGT CATCATCAGA CTGGAAGTCT
    CGCCCCCTCC TTCATCAGCT GCCCAACACA AACACAACAG CGTCCAGGCC TCAGTTCAAC
    AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330758.1
    CDS42..4070
    Translation

    Target ORF information:

    RefSeq Version XM_021475083.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAACAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGGC CCGGATTACA GGCTGTATAA GAGTGAGCCG
    GAGCTGACCA CAGTAGCCGA AGTCGACGAG AGTAACGGTG AAGACAAATC AGAGCAGACA
    GCAGAGAAAG AGCCGCCCGG CGTCACTAAA GGTATAGTTT ACCCGGTGGG AGTGGTCAGT
    CCCAGAACTA AATCACCCAT GCCCGAGTCC TCCACCATCG CCTCCTACGT CACCTTGAGG
    AAGACCAAAA AACCTGATCC CAGAACATGT CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG
    GAGCAGATGA GCAGTGGTGC TGAAGTGGGC CGAACACGCA TGAGTGTGGA GGAGCAGATG
    GAGAGAATCA GACGACACCA GCAGGGGGCG CTGAGAGAGC GCAGGAGAGA AGATGGCTCT
    CTATCCCGCA GCCTGTCCTT CACTAAGGAC AGCACATACT ACACACTGCA GGCACGTAAG
    AAGAGTCCGG TGATCTCTCC AGACGAGCAG GACGAGCGCA GAGACATGAC GCCTGTGGAG
    CGAGACACAA AACACACAAA ACACCCTGAC GCAGAGGAAA ACTGCAGAGA CACTAAGGAG
    CAGCTGCCAG CACACACCCA CCTGGAGATT AAGGACAAAG CATCTGTGAC TCGCTCGGTC
    TCCATGAAAG AGTCTCTCCT GAAGAGTGTG AGTCCCAAAC CCGTGCTGAA GCAGGACGAA
    TCTGAGTGTA AACTGGAGTA CGACAGAAAG CTGGCCACTC AGAACAGCCT GCAGACGGCA
    GTGATGGTGC GTGTGGGCAC TGAGGAGGAT GAAGAGGATG AAGAAGAGGA GCAGAAAGAC
    TCAGTTCAGC AGCAGGAGGA CTCTTATGAG CTCAGCAACA GCAAACACAG CACACTGATC
    TCAGCATCAT GTGACAGGAA GAACACATCA TCACCATCGT CATCATCAGA CTGGAAGTCT
    CGCCCCCTCC TTCATCAGCT GCCCAACACA AACACAACAG CGTCCAGGCC TCAGTTCAAC
    AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142027
    Clone ID Related Accession (Same CDS sequence) XM_021475084.1
    Accession Version XM_021475084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACACCTG
    AATTGTGGGA ATCTTGTCCA TCCTGTTTTA AGCAGACCAG CGACCAAAGC ACCAGAGGCG
    GAGAAGAAGG AGCGTCCCAC CAGCACCATG AGCGAAGCCT CAAACTACAC CGGCGGCTCC
    GACTACACCA CACATCCCAG CAGCCCCACA ACCAGACCAT CCCGGTCCTC AAAGAAAGTG
    CACAACTTTG GGAAAAGGTC CAACTCCATC AAGAGGAACC CCAATGCACC TGTGATCAAA
    AACGGCTGGC TTCACAAACA GGACAGCACT GGGATGAAGA TGTGGAAGAA GAGATGGTTC
    GTTCTGTCTG ATATGTGCCT CTTCTACTAC AGAGATGAGA AGGAAGAGGG GATTCTGGGA
    AGCATCCTCC TGCCTAGCTT TCACATCTCC ATGCTGTCCG TGGACGACCA TATTACCCGG
    AAATACGCCT TCAAGGCGAC GCATCCAAAC ATGCGGACGT ATTATTTCAG CACAGACACA
    GCCAAAGATA TGGAGTCCTG GATGAAAGTG ATGTCGGATG CGGCGATGGC TCACAGCGAG
    CCAATCAGAA GGCTTGAGAA GGTAAAGCTG GACTCTCGCG TTCCTCAGGA GATGAACAAC
    ATGCTGAACC ACCGCGTCTT GACACGACCC GAGATCCAAA ACAACGAGAG GAACCGCGAG
    ACGCTCCGCC AAGAGGACAA GAAGAGCAAA CCATCAGAGA AACCTCAGAA AGACAAAGAC
    AGAGTCAGCA TGCAGAAAGA CGGAGAGCGC TTCAGTGAAA ACTACACTCA CCGGCAGAAA
    GACGGCGAGC GCTACATGCT GCAGAAAGAC CTGGAGAAAT TTGTCCTGCA GAAAGACGGA
    GAGAAGTACG CCGTGCAGAA AGAAGGCGAC AAATATCTCC TGCAGAAAGA CGGACACAAA
    TACACAGTGC ACAAAGACGG AGACAAATAC ACGCTTCAGA GAGAACTCGA GATGTACAAT
    CCACCAAAAG ACGCGGAGAA GATCCCGCAG AAAGATGCAA GGTTTAGTCT GGTGCAGAAG
    GAGCTGGAGA AACCCGTCCC GCCACCGCGA TCTGAGGAAA AATACGGCTT TCAGAAAGAG
    GGCACCATTG AGCGTCCGCT AACTAAAATA AATAGCATTA AACTGCAGCC GGCTCAAGCA
    GCCGCCATCG CTGCAGCCGT TTCATCCTCT AGGATGATGC AGAAACAGGT TAATGGCTCC
    GGGGAACGAG CGGGAGACAG AAGTCCTGGA GATTTGAGTA CTGTGAGGAC GAGCGTCCCG
    CCACCAACGC AGCCGGCTCC AGAACCAGAG CGAAGCCTCA GCAGAACCAG CTCAATGCAG
    CAGCTGGAGC AGTGGGTTCG CACACACCGC ACACGAGCTC CAGACGACGA CACCAGGAGC
    ATTACGTCCT ACCAGACCCT GCCAAGGAAC ATGCCGAGCC ACCGGGCGCA GATTGTCCCT
    CGCTACCCTG AGGGCTACCG CACTCTGCCC CGAAACATGC TGCGTCCGGA CAGCATCTGC
    AGTGTAGCAG GATCAGTGTA CGACCGAGCG CTTCAGCCCA GCACGCAGGA AAAGCGGCGC
    TCCATCCGAG ATGACACCAT GTGGCAGCTG TACGAGTGGC AGCAGCGGCA GGCGCACAGT
    CGGCTTGGGT ACGGGACGCT GCCCAGTCCC AAAACTATGG GGCAGATCGC GGAGTCCATC
    CCCACGTCCC CATCCCATGG CTCTCTGGCC GGATATCACA CCTTCTCCCC GAACAGACCG
    CTCAACCCTG ACTCGCGCTC GGAGCTGTCT TCGCCTGTGT TTCGTGGAGA CGTGACCATC
    GAGCGACGCC ACCGACCACA CCTGTCCAAG TACTCCTATC CTGCGGAACG CAGAGCTGCT
    CCTCCCGCAC AGAACATCAC TGCACAGTCT CTTCAAGGCA AAACGCCAGA GGAACTGACC
    CTGCTGCTCA TCAAACTGCG GCGGCAGCAG GCCGAGCTCA ACAGCCTCCG TGAGCACACA
    CTCGCGCAGC TCATGCAGCT GAACCTCGAC GCCACCAACC CAAAGAGTGA AATCCTCTCT
    CATCACCTGC AGAGGAATCT CATGTACTTG GACAGTCAGA TGAAGGAGAA CGAACCCATA
    ATCTTCATGA TTCACACTAT GATTGAGAAC TCGGCGCCCC GGCCGCAGCT CTACCAGCAA
    CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT
    GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG
    AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT
    CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG
    GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG
    CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT
    AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG
    CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG
    AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG
    CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC
    TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC
    CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC
    GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC
    GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT
    AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG
    TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA
    TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG
    GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG
    GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG
    GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG
    CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT
    GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG
    ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT
    GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA
    AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG
    GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT
    GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA
    TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC
    ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330759.1
    CDS75..4076
    Translation

    Target ORF information:

    RefSeq Version XM_021475084.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACACCTG
    AATTGTGGGA ATCTTGTCCA TCCTGTTTTA AGCAGACCAG CGACCAAAGC ACCAGAGGCG
    GAGAAGAAGG AGCGTCCCAC CAGCACCATG AGCGAAGCCT CAAACTACAC CGGCGGCTCC
    GACTACACCA CACATCCCAG CAGCCCCACA ACCAGACCAT CCCGGTCCTC AAAGAAAGTG
    CACAACTTTG GGAAAAGGTC CAACTCCATC AAGAGGAACC CCAATGCACC TGTGATCAAA
    AACGGCTGGC TTCACAAACA GGACAGCACT GGGATGAAGA TGTGGAAGAA GAGATGGTTC
    GTTCTGTCTG ATATGTGCCT CTTCTACTAC AGAGATGAGA AGGAAGAGGG GATTCTGGGA
    AGCATCCTCC TGCCTAGCTT TCACATCTCC ATGCTGTCCG TGGACGACCA TATTACCCGG
    AAATACGCCT TCAAGGCGAC GCATCCAAAC ATGCGGACGT ATTATTTCAG CACAGACACA
    GCCAAAGATA TGGAGTCCTG GATGAAAGTG ATGTCGGATG CGGCGATGGC TCACAGCGAG
    CCAATCAGAA GGCTTGAGAA GGTAAAGCTG GACTCTCGCG TTCCTCAGGA GATGAACAAC
    ATGCTGAACC ACCGCGTCTT GACACGACCC GAGATCCAAA ACAACGAGAG GAACCGCGAG
    ACGCTCCGCC AAGAGGACAA GAAGAGCAAA CCATCAGAGA AACCTCAGAA AGACAAAGAC
    AGAGTCAGCA TGCAGAAAGA CGGAGAGCGC TTCAGTGAAA ACTACACTCA CCGGCAGAAA
    GACGGCGAGC GCTACATGCT GCAGAAAGAC CTGGAGAAAT TTGTCCTGCA GAAAGACGGA
    GAGAAGTACG CCGTGCAGAA AGAAGGCGAC AAATATCTCC TGCAGAAAGA CGGACACAAA
    TACACAGTGC ACAAAGACGG AGACAAATAC ACGCTTCAGA GAGAACTCGA GATGTACAAT
    CCACCAAAAG ACGCGGAGAA GATCCCGCAG AAAGATGCAA GGTTTAGTCT GGTGCAGAAG
    GAGCTGGAGA AACCCGTCCC GCCACCGCGA TCTGAGGAAA AATACGGCTT TCAGAAAGAG
    GGCACCATTG AGCGTCCGCT AACTAAAATA AATAGCATTA AACTGCAGCC GGCTCAAGCA
    GCCGCCATCG CTGCAGCCGT TTCATCCTCT AGGATGATGC AGAAACAGGT TAATGGCTCC
    GGGGAACGAG CGGGAGACAG AAGTCCTGGA GATTTGAGTA CTGTGAGGAC GAGCGTCCCG
    CCACCAACGC AGCCGGCTCC AGAACCAGAG CGAAGCCTCA GCAGAACCAG CTCAATGCAG
    CAGCTGGAGC AGTGGGTTCG CACACACCGC ACACGAGCTC CAGACGACGA CACCAGGAGC
    ATTACGTCCT ACCAGACCCT GCCAAGGAAC ATGCCGAGCC ACCGGGCGCA GATTGTCCCT
    CGCTACCCTG AGGGCTACCG CACTCTGCCC CGAAACATGC TGCGTCCGGA CAGCATCTGC
    AGTGTAGCAG GATCAGTGTA CGACCGAGCG CTTCAGCCCA GCACGCAGGA AAAGCGGCGC
    TCCATCCGAG ATGACACCAT GTGGCAGCTG TACGAGTGGC AGCAGCGGCA GGCGCACAGT
    CGGCTTGGGT ACGGGACGCT GCCCAGTCCC AAAACTATGG GGCAGATCGC GGAGTCCATC
    CCCACGTCCC CATCCCATGG CTCTCTGGCC GGATATCACA CCTTCTCCCC GAACAGACCG
    CTCAACCCTG ACTCGCGCTC GGAGCTGTCT TCGCCTGTGT TTCGTGGAGA CGTGACCATC
    GAGCGACGCC ACCGACCACA CCTGTCCAAG TACTCCTATC CTGCGGAACG CAGAGCTGCT
    CCTCCCGCAC AGAACATCAC TGCACAGTCT CTTCAAGGCA AAACGCCAGA GGAACTGACC
    CTGCTGCTCA TCAAACTGCG GCGGCAGCAG GCCGAGCTCA ACAGCCTCCG TGAGCACACA
    CTCGCGCAGC TCATGCAGCT GAACCTCGAC GCCACCAACC CAAAGAGTGA AATCCTCTCT
    CATCACCTGC AGAGGAATCT CATGTACTTG GACAGTCAGA TGAAGGAGAA CGAACCCATA
    ATCTTCATGA TTCACACTAT GATTGAGAAC TCGGCGCCCC GGCCGCAGCT CTACCAGCAA
    CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT
    GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG
    AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT
    CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG
    GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG
    CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT
    AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG
    CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG
    AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG
    CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC
    TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC
    CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC
    GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC
    GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT
    AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG
    TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA
    TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG
    GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG
    GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG
    GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG
    CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT
    GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG
    ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT
    GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA
    AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG
    GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT
    GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA
    TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC
    ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142028
    Clone ID Related Accession (Same CDS sequence) XM_021475085.1
    Accession Version XM_021475085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG
    AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC
    GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG
    CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG
    ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC
    CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG
    CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG
    CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT
    CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA
    CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA
    AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT
    GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA
    ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG
    GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT
    AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT
    ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC
    ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG
    CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA
    ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG
    AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC
    ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC
    GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA
    GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG
    GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT
    CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG
    GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA
    GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC
    AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA
    CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC
    ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330760.1
    CDS42..4037
    Translation

    Target ORF information:

    RefSeq Version XM_021475085.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGCAGATG
    AGTCCTGAAG AGTACAGAGA AAACACTTAC ATGTACAGGC CGGAGGAGCT CGATGTCGAC
    GCGAAGTTAA GTCGGCTCTG TGAGCAGGAC AAAGTAGTGC GAACTCAAGA AGAGAAACTG
    CAGCAGCTCC ACAGAGAAAA GCACACACTG GAGACGGCGC TGCTCTCCGC CAGTCAGGAG
    ATCGAGATGA GCTCTGAAAA CCCAGCGGCG GTCCAGAGCG TGGTCCAGCA GAGGGACGTC
    CTGCAGAGTG GCCTGCTGAG CACCTGCAGA GAGCTGAGCC GCGTCAGCAC TGAGCTGGAG
    CGAAGCTGGA GAGAATATGA TCGACTAGAG GCCGATGTTA CACTTGCCAA AACTAACCTG
    CTGGAGCAGC TGGAGGCCCT CGGCAGCCCA CAGACGGAGC CGCCCAGTCA AAAGCATGTT
    CAGATCCAGA AAGAGTTGTG GAGAATTCAG GATGTGATGG AGGCGCTGAG CAAGAACAAA
    CCCAAGAGAA ACCCTGAACC CAGTTTTCCT GGTGCAAACC CTCTGTCAAC CCTGCATAAA
    AGTGAAGACT CTGTAGACTC CTCGTGTGTT CCTCCTCGTC CTCCTCTGCC GTACTCATAT
    GAAGGAGGCG ATCGACCACC GCCCCTCCCC AACCCCGGTC AACCGCACAC CACCCGCACA
    ACACCACACC GACCAGAGGA CCGCAAAACT GCCCAACGCA ACGGCACGCA CACCGGCCCG
    GATTACAGGC TGTATAAGAG TGAGCCGGAG CTGACCACAG TAGCCGAAGT CGACGAGAGT
    AACGGTGAAG ACAAATCAGA GCAGACAGCA GAGAAAGAGC CGCCCGGCGT CACTAAAGGT
    ATAGTTTACC CGGTGGGAGT GGTCAGTCCC AGAACTAAAT CACCCATGCC CGAGTCCTCC
    ACCATCGCCT CCTACGTCAC CTTGAGGAAG ACCAAAAAAC CTGATCCCAG AACATGTCAG
    CCGCAGGACC GTCCGCGCAG CGCTGTGGAG CAGATGAGCA GTGGTGCTGA AGTGGGCCGA
    ACACGCATGA GTGTGGAGGA GCAGATGGAG AGAATCAGAC GACACCAGCA GGGGGCGCTG
    AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA TCCCGCAGCC TGTCCTTCAC TAAGGACAGC
    ACATACTACA CACTGCAGGC ACGTAAGAAG AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC
    GAGCGCAGAG ACATGACGCC TGTGGAGCGA GACACAAAAC ACACAAAACA CCCTGACGCA
    GAGGAAAACT GCAGAGACAC TAAGGAGCAG CTGCCAGCAC ACACCCACCT GGAGATTAAG
    GACAAAGCAT CTGTGACTCG CTCGGTCTCC ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT
    CCCAAACCCG TGCTGAAGCA GGACGAATCT GAGTGTAAAC TGGAGTACGA CAGAAAGCTG
    GCCACTCAGA ACAGCCTGCA GACGGCAGTG ATGGTGCGTG TGGGCACTGA GGAGGATGAA
    GAGGATGAAG AAGAGGAGCA GAAAGACTCA GTTCAGCAGC AGGAGGACTC TTATGAGCTC
    AGCAACAGCA AACACAGCAC ACTGATCTCA GCATCATGTG ACAGGAAGAA CACATCATCA
    CCATCGTCAT CATCAGACTG GAAGTCTCGC CCCCTCCTTC ATCAGCTGCC CAACACAAAC
    ACAACAGCGT CCAGGCCTCA GTTCAACAGC GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142029
    Clone ID Related Accession (Same CDS sequence) XM_021475086.1
    Accession Version XM_021475086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA
    GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA
    CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT
    TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC
    GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA
    GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC
    ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG
    CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA
    CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA
    GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA
    TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG
    CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG
    GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC
    AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC
    AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC
    ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG
    GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC
    AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA
    TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA
    ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330761.1
    CDS42..4034
    Translation

    Target ORF information:

    RefSeq Version XM_021475086.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGACTCTG TAGACTCCTC GTGTGTTCCT CCTCGTCCTC CTCTGCCGTA CTCATATGAA
    GGAGGCGATC GACCACCGCC CCTCCCCAAC CCCGGTCAAC CGCACACCAC CCGCACAACA
    CCACACCGAC CAGAGGACCG CAAAACTGCC CAACGCAACG GCACGCACAC CGGCCCGGAT
    TACAGGCTGT ATAAGAGTGA GCCGGAGCTG ACCACAGTAG CCGAAGTCGA CGAGAGTAAC
    GGTGAAGACA AATCAGAGCA GACAGCAGAG AAAGAGCCGC CCGGCGTCAC TAAAGGTATA
    GTTTACCCGG TGGGAGTGGT CAGTCCCAGA ACTAAATCAC CCATGCCCGA GTCCTCCACC
    ATCGCCTCCT ACGTCACCTT GAGGAAGACC AAAAAACCTG ATCCCAGAAC ATGTCAGCCG
    CAGGACCGTC CGCGCAGCGC TGTGGAGCAG ATGAGCAGTG GTGCTGAAGT GGGCCGAACA
    CGCATGAGTG TGGAGGAGCA GATGGAGAGA ATCAGACGAC ACCAGCAGGG GGCGCTGAGA
    GAGCGCAGGA GAGAAGATGG CTCTCTATCC CGCAGCCTGT CCTTCACTAA GGACAGCACA
    TACTACACAC TGCAGGCACG TAAGAAGAGT CCGGTGATCT CTCCAGACGA GCAGGACGAG
    CGCAGAGACA TGACGCCTGT GGAGCGAGAC ACAAAACACA CAAAACACCC TGACGCAGAG
    GAAAACTGCA GAGACACTAA GGAGCAGCTG CCAGCACACA CCCACCTGGA GATTAAGGAC
    AAAGCATCTG TGACTCGCTC GGTCTCCATG AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC
    AAACCCGTGC TGAAGCAGGA CGAATCTGAG TGTAAACTGG AGTACGACAG AAAGCTGGCC
    ACTCAGAACA GCCTGCAGAC GGCAGTGATG GTGCGTGTGG GCACTGAGGA GGATGAAGAG
    GATGAAGAAG AGGAGCAGAA AGACTCAGTT CAGCAGCAGG AGGACTCTTA TGAGCTCAGC
    AACAGCAAAC ACAGCACACT GATCTCAGCA TCATGTGACA GGAAGAACAC ATCATCACCA
    TCGTCATCAT CAGACTGGAA GTCTCGCCCC CTCCTTCATC AGCTGCCCAA CACAAACACA
    ACAGCGTCCA GGCCTCAGTT CAACAGCGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142030
    Clone ID Related Accession (Same CDS sequence) XM_021475087.1
    Accession Version XM_021475087.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
    GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC ACCTGAATTG TGGGAATCTT
    GTCCATCCTG TTTTAAGCAG ACCAGCGACC AAAGCACCAG AGGCGGAGAA GAAGGAGCGT
    CCCACCAGCA CCATGAGCGA AGCCTCAAAC TACACCGGCG GCTCCGACTA CACCACACAT
    CCCAGCAGCC CCACAACCAG ACCATCCCGG TCCTCAAAGA AAGTGCACAA CTTTGGGAAA
    AGGTCCAACT CCATCAAGAG GAACCCCAAT GCACCTGTGA TCAAAAACGG CTGGCTTCAC
    AAACAGGACA GCACTGGGAT GAAGATGTGG AAGAAGAGAT GGTTCGTTCT GTCTGATATG
    TGCCTCTTCT ACTACAGAGA TGAGAAGGAA GAGGGGATTC TGGGAAGCAT CCTCCTGCCT
    AGCTTTCACA TCTCCATGCT GTCCGTGGAC GACCATATTA CCCGGAAATA CGCCTTCAAG
    GCGACGCATC CAAACATGCG GACGTATTAT TTCAGCACAG ACACAGCCAA AGATATGGAG
    TCCTGGATGA AAGTGATGTC GGATGCGGCG ATGGCTCACA GCGAGCCAAT CAGAAGGCTT
    GAGAAGGTAA AGCTGGACTC TCGCGTTCCT CAGGAGATGA ACAACATGCT GAACCACCGC
    GTCTTGACAC GACCCGAGAT CCAAAACAAC GAGAGGAACC GCGAGACGCT CCGCCAAGAG
    GACAAGAAGA GCAAACCATC AGAGAAACCT CAGAAAGACA AAGACAGAGT CAGCATGCAG
    AAAGACGGAG AGCGCTTCAG TGAAAACTAC ACTCACCGGC AGAAAGACGG CGAGCGCTAC
    ATGCTGCAGA AAGACCTGGA GAAATTTGTC CTGCAGAAAG ACGGAGAGAA GTACGCCGTG
    CAGAAAGAAG GCGACAAATA TCTCCTGCAG AAAGACGGAC ACAAATACAC AGTGCACAAA
    GACGGAGACA AATACACGCT TCAGAGAGAA CTCGAGATGT ACAATCCACC AAAAGACGCG
    GAGAAGATCC CGCAGAAAGA TGCAAGGTTT AGTCTGGTGC AGAAGGAGCT GGAGAAACCC
    GTCCCGCCAC CGCGATCTGA GGAAAAATAC GGCTTTCAGA AAGAGGGCAC CATTGAGCGT
    CCGCTAACTA AAATAAATAG CATTAAACTG CAGCCGGCTC AAGCAGCCGC CATCGCTGCA
    GCCGTTTCAT CCTCTAGGAT GATGCAGAAA CAGGTTAATG GCTCCGGGGA ACGAGCGGGA
    GACAGAAGTC CTGGAGATTT GAGTACTGTG AGGACGAGCG TCCCGCCACC AACGCAGCCG
    GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG
    GTTCGCACAC ACCGCACACG AGCTCCAGAC GACGACACCA GGAGCATTAC GTCCTACCAG
    ACCCTGCCAA GGAACATGCC GAGCCACCGG GCGCAGATTG TCCCTCGCTA CCCTGAGGGC
    TACCGCACTC TGCCCCGAAA CATGCTGCGT CCGGACAGCA TCTGCAGTGT AGCAGGATCA
    GTGTACGACC GAGCGCTTCA GCCCAGCACG CAGGAAAAGC GGCGCTCCAT CCGAGATGAC
    ACCATGTGGC AGCTGTACGA GTGGCAGCAG CGGCAGGCGC ACAGTCGGCT TGGGTACGGG
    ACGCTGCCCA GTCCCAAAAC TATGGGGCAG ATCGCGGAGT CCATCCCCAC GTCCCCATCC
    CATGGCTCTC TGGCCGGATA TCACACCTTC TCCCCGAACA GACCGCTCAA CCCTGACTCG
    CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT GGAGACGTGA CCATCGAGCG ACGCCACCGA
    CCACACCTGT CCAAGTACTC CTATCCTGCG GAACGCAGAG CTGCTCCTCC CGCACAGAAC
    ATCACTGCAC AGTCTCTTCA AGGCAAAACG CCAGAGGAAC TGACCCTGCT GCTCATCAAA
    CTGCGGCGGC AGCAGGCCGA GCTCAACAGC CTCCGTGAGC ACACACTCGC GCAGCTCATG
    CAGCTGAACC TCGACGCCAC CAACCCAAAG AGTGAAATCC TCTCTCATCA CCTGCAGAGG
    AATCTCATGT ACTTGGACAG TCAGATGAAG GAGAACGAAC CCATAATCTT CATGATTCAC
    ACTATGATTG AGAACTCGGC GCCCCGGCCG CAGCTCTACC AGCAACAGAT GAGTCCTGAA
    GAGTACAGAG AAAACACTTA CATGTACAGG CCGGAGGAGC TCGATGTCGA CGCGAAGTTA
    AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG CGAACTCAAG AAGAGAAACT GCAGCAGCTC
    CACAGAGAAA AGCACACACT GGAGACGGCG CTGCTCTCCG CCAGTCAGGA GATCGAGATG
    AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC GTGGTCCAGC AGAGGGACGT CCTGCAGAGT
    GGCCTGCTGA GCACCTGCAG AGAGCTGAGC CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG
    AGAGAATATG ATCGACTAGA GGCCGATGTT ACACTTGCCA AAACTAACCT GCTGGAGCAG
    CTGGAGGCCC TCGGCAGCCC ACAGACGGAG CCGCCCAGTC AAAAGCATGT TCAGATCCAG
    AAAGAGTTGT GGAGAATTCA GGATGTGATG GAGGCGCTGA GCAAGAACAA ACCCAAGAGA
    AACCCTGAAC CCAGTTTTCC TGGTGCAAAC CCTCTGTCAA CCCTGCATAA AAGTGAAGAC
    TCTGTAGACT CCTCGTGTGT TCCTCCTCGT CCTCCTCTGC CGTACTCATA TGAAGGAGGC
    GATCGACCAC CGCCCCTCCC CAACCCCGGT CAACCGCACA CCACCCGCAC AACACCACAC
    CGACCAGAGG ACCGCAAAAC TGCCCAACGC AACGGCACGC ACACCGGCCC GGATTACAGG
    CTGTATAAGA GTGAGCCGGA GCTGACCACA GTAGCCGAAG TCGACGAGAG TAACGGTGAA
    GACAAATCAG AGCAGACAGC AGAGAAAGAG CCGCCCGGCG TCACTAAAGG TATAGTTTAC
    CCGGTGGGAG TGGTCAGTCC CAGAACTAAA TCACCCATGC CCGAGTCCTC CACCATCGCC
    TCCTACGTCA CCTTGAGGAA GACCAAAAAA CCTGATCCCA GAACATGTCA GCCGCAGGAC
    CGTCCGCGCA GCGCTGTGGA GCAGATGAGC AGTGGTGCTG AAGTGGGCCG AACACGCATG
    AGTGTGGAGG AGCAGATGGA GAGAATCAGA CGACACCAGC AGGGGGCGCT GAGAGAGCGC
    AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC CTGTCCTTCA CTAAGGACAG CACATACTAC
    ACACTGCAGG CACGTAAGAA GAGTCCGGTG ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA
    GACATGACGC CTGTGGAGCG AGACACAAAA CACACAAAAC ACCCTGACGC AGAGGAAAAC
    TGCAGAGACA CTAAGGAGCA GCTGCCAGCA CACACCCACC TGGAGATTAA GGACAAAGCA
    TCTGTGACTC GCTCGGTCTC CATGAAAGAG TCTCTCCTGA AGAGTGTGAG TCCCAAACCC
    GTGCTGAAGC AGGACGAATC TGAGTGTAAA CTGGAGTACG ACAGAAAGCT GGCCACTCAG
    AACAGCCTGC AGACGGCAGT GATGGTGCGT GTGGGCACTG AGGAGGATGA AGAGGATGAA
    GAAGAGGAGC AGAAAGACTC AGTTCAGCAG CAGGAGGACT CTTATGAGCT CAGCAACAGC
    AAACACAGCA CACTGATCTC AGCATCATGT GACAGGAAGA ACACATCATC ACCATCGTCA
    TCATCAGACT GGAAGTCTCG CCCCCTCCTT CATCAGCTGC CCAACACAAA CACAACAGCG
    TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330762.1
    CDS117..4103
    Translation

    Target ORF information:

    RefSeq Version XM_021475087.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475087.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
    GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC ACCTGAATTG TGGGAATCTT
    GTCCATCCTG TTTTAAGCAG ACCAGCGACC AAAGCACCAG AGGCGGAGAA GAAGGAGCGT
    CCCACCAGCA CCATGAGCGA AGCCTCAAAC TACACCGGCG GCTCCGACTA CACCACACAT
    CCCAGCAGCC CCACAACCAG ACCATCCCGG TCCTCAAAGA AAGTGCACAA CTTTGGGAAA
    AGGTCCAACT CCATCAAGAG GAACCCCAAT GCACCTGTGA TCAAAAACGG CTGGCTTCAC
    AAACAGGACA GCACTGGGAT GAAGATGTGG AAGAAGAGAT GGTTCGTTCT GTCTGATATG
    TGCCTCTTCT ACTACAGAGA TGAGAAGGAA GAGGGGATTC TGGGAAGCAT CCTCCTGCCT
    AGCTTTCACA TCTCCATGCT GTCCGTGGAC GACCATATTA CCCGGAAATA CGCCTTCAAG
    GCGACGCATC CAAACATGCG GACGTATTAT TTCAGCACAG ACACAGCCAA AGATATGGAG
    TCCTGGATGA AAGTGATGTC GGATGCGGCG ATGGCTCACA GCGAGCCAAT CAGAAGGCTT
    GAGAAGGTAA AGCTGGACTC TCGCGTTCCT CAGGAGATGA ACAACATGCT GAACCACCGC
    GTCTTGACAC GACCCGAGAT CCAAAACAAC GAGAGGAACC GCGAGACGCT CCGCCAAGAG
    GACAAGAAGA GCAAACCATC AGAGAAACCT CAGAAAGACA AAGACAGAGT CAGCATGCAG
    AAAGACGGAG AGCGCTTCAG TGAAAACTAC ACTCACCGGC AGAAAGACGG CGAGCGCTAC
    ATGCTGCAGA AAGACCTGGA GAAATTTGTC CTGCAGAAAG ACGGAGAGAA GTACGCCGTG
    CAGAAAGAAG GCGACAAATA TCTCCTGCAG AAAGACGGAC ACAAATACAC AGTGCACAAA
    GACGGAGACA AATACACGCT TCAGAGAGAA CTCGAGATGT ACAATCCACC AAAAGACGCG
    GAGAAGATCC CGCAGAAAGA TGCAAGGTTT AGTCTGGTGC AGAAGGAGCT GGAGAAACCC
    GTCCCGCCAC CGCGATCTGA GGAAAAATAC GGCTTTCAGA AAGAGGGCAC CATTGAGCGT
    CCGCTAACTA AAATAAATAG CATTAAACTG CAGCCGGCTC AAGCAGCCGC CATCGCTGCA
    GCCGTTTCAT CCTCTAGGAT GATGCAGAAA CAGGTTAATG GCTCCGGGGA ACGAGCGGGA
    GACAGAAGTC CTGGAGATTT GAGTACTGTG AGGACGAGCG TCCCGCCACC AACGCAGCCG
    GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG
    GTTCGCACAC ACCGCACACG AGCTCCAGAC GACGACACCA GGAGCATTAC GTCCTACCAG
    ACCCTGCCAA GGAACATGCC GAGCCACCGG GCGCAGATTG TCCCTCGCTA CCCTGAGGGC
    TACCGCACTC TGCCCCGAAA CATGCTGCGT CCGGACAGCA TCTGCAGTGT AGCAGGATCA
    GTGTACGACC GAGCGCTTCA GCCCAGCACG CAGGAAAAGC GGCGCTCCAT CCGAGATGAC
    ACCATGTGGC AGCTGTACGA GTGGCAGCAG CGGCAGGCGC ACAGTCGGCT TGGGTACGGG
    ACGCTGCCCA GTCCCAAAAC TATGGGGCAG ATCGCGGAGT CCATCCCCAC GTCCCCATCC
    CATGGCTCTC TGGCCGGATA TCACACCTTC TCCCCGAACA GACCGCTCAA CCCTGACTCG
    CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT GGAGACGTGA CCATCGAGCG ACGCCACCGA
    CCACACCTGT CCAAGTACTC CTATCCTGCG GAACGCAGAG CTGCTCCTCC CGCACAGAAC
    ATCACTGCAC AGTCTCTTCA AGGCAAAACG CCAGAGGAAC TGACCCTGCT GCTCATCAAA
    CTGCGGCGGC AGCAGGCCGA GCTCAACAGC CTCCGTGAGC ACACACTCGC GCAGCTCATG
    CAGCTGAACC TCGACGCCAC CAACCCAAAG AGTGAAATCC TCTCTCATCA CCTGCAGAGG
    AATCTCATGT ACTTGGACAG TCAGATGAAG GAGAACGAAC CCATAATCTT CATGATTCAC
    ACTATGATTG AGAACTCGGC GCCCCGGCCG CAGCTCTACC AGCAACAGAT GAGTCCTGAA
    GAGTACAGAG AAAACACTTA CATGTACAGG CCGGAGGAGC TCGATGTCGA CGCGAAGTTA
    AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG CGAACTCAAG AAGAGAAACT GCAGCAGCTC
    CACAGAGAAA AGCACACACT GGAGACGGCG CTGCTCTCCG CCAGTCAGGA GATCGAGATG
    AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC GTGGTCCAGC AGAGGGACGT CCTGCAGAGT
    GGCCTGCTGA GCACCTGCAG AGAGCTGAGC CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG
    AGAGAATATG ATCGACTAGA GGCCGATGTT ACACTTGCCA AAACTAACCT GCTGGAGCAG
    CTGGAGGCCC TCGGCAGCCC ACAGACGGAG CCGCCCAGTC AAAAGCATGT TCAGATCCAG
    AAAGAGTTGT GGAGAATTCA GGATGTGATG GAGGCGCTGA GCAAGAACAA ACCCAAGAGA
    AACCCTGAAC CCAGTTTTCC TGGTGCAAAC CCTCTGTCAA CCCTGCATAA AAGTGAAGAC
    TCTGTAGACT CCTCGTGTGT TCCTCCTCGT CCTCCTCTGC CGTACTCATA TGAAGGAGGC
    GATCGACCAC CGCCCCTCCC CAACCCCGGT CAACCGCACA CCACCCGCAC AACACCACAC
    CGACCAGAGG ACCGCAAAAC TGCCCAACGC AACGGCACGC ACACCGGCCC GGATTACAGG
    CTGTATAAGA GTGAGCCGGA GCTGACCACA GTAGCCGAAG TCGACGAGAG TAACGGTGAA
    GACAAATCAG AGCAGACAGC AGAGAAAGAG CCGCCCGGCG TCACTAAAGG TATAGTTTAC
    CCGGTGGGAG TGGTCAGTCC CAGAACTAAA TCACCCATGC CCGAGTCCTC CACCATCGCC
    TCCTACGTCA CCTTGAGGAA GACCAAAAAA CCTGATCCCA GAACATGTCA GCCGCAGGAC
    CGTCCGCGCA GCGCTGTGGA GCAGATGAGC AGTGGTGCTG AAGTGGGCCG AACACGCATG
    AGTGTGGAGG AGCAGATGGA GAGAATCAGA CGACACCAGC AGGGGGCGCT GAGAGAGCGC
    AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC CTGTCCTTCA CTAAGGACAG CACATACTAC
    ACACTGCAGG CACGTAAGAA GAGTCCGGTG ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA
    GACATGACGC CTGTGGAGCG AGACACAAAA CACACAAAAC ACCCTGACGC AGAGGAAAAC
    TGCAGAGACA CTAAGGAGCA GCTGCCAGCA CACACCCACC TGGAGATTAA GGACAAAGCA
    TCTGTGACTC GCTCGGTCTC CATGAAAGAG TCTCTCCTGA AGAGTGTGAG TCCCAAACCC
    GTGCTGAAGC AGGACGAATC TGAGTGTAAA CTGGAGTACG ACAGAAAGCT GGCCACTCAG
    AACAGCCTGC AGACGGCAGT GATGGTGCGT GTGGGCACTG AGGAGGATGA AGAGGATGAA
    GAAGAGGAGC AGAAAGACTC AGTTCAGCAG CAGGAGGACT CTTATGAGCT CAGCAACAGC
    AAACACAGCA CACTGATCTC AGCATCATGT GACAGGAAGA ACACATCATC ACCATCGTCA
    TCATCAGACT GGAAGTCTCG CCCCCTCCTT CATCAGCTGC CCAACACAAA CACAACAGCG
    TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142031
    Clone ID Related Accession (Same CDS sequence) XM_021475088.1
    Accession Version XM_021475088.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3945bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGGCCCGG ATTACAGGCT GTATAAGAGT GAGCCGGAGC TGACCACAGT AGCCGAAGTC
    GACGAGAGTA ACGGTGAAGA CAAATCAGAG CAGACAGCAG AGAAAGAGCC GCCCGGCGTC
    ACTAAAGGTA TAGTTTACCC GGTGGGAGTG GTCAGTCCCA GAACTAAATC ACCCATGCCC
    GAGTCCTCCA CCATCGCCTC CTACGTCACC TTGAGGAAGA CCAAAAAACC TGATCCCAGA
    ACATGTCAGC CGCAGGACCG TCCGCGCAGC GCTGTGGAGC AGATGAGCAG TGGTGCTGAA
    GTGGGCCGAA CACGCATGAG TGTGGAGGAG CAGATGGAGA GAATCAGACG ACACCAGCAG
    GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT GGCTCTCTAT CCCGCAGCCT GTCCTTCACT
    AAGGACAGCA CATACTACAC ACTGCAGGCA CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC
    GAGCAGGACG AGCGCAGAGA CATGACGCCT GTGGAGCGAG ACACAAAACA CACAAAACAC
    CCTGACGCAG AGGAAAACTG CAGAGACACT AAGGAGCAGC TGCCAGCACA CACCCACCTG
    GAGATTAAGG ACAAAGCATC TGTGACTCGC TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG
    AGTGTGAGTC CCAAACCCGT GCTGAAGCAG GACGAATCTG AGTGTAAACT GGAGTACGAC
    AGAAAGCTGG CCACTCAGAA CAGCCTGCAG ACGGCAGTGA TGGTGCGTGT GGGCACTGAG
    GAGGATGAAG AGGATGAAGA AGAGGAGCAG AAAGACTCAG TTCAGCAGCA GGAGGACTCT
    TATGAGCTCA GCAACAGCAA ACACAGCACA CTGATCTCAG CATCATGTGA CAGGAAGAAC
    ACATCATCAC CATCGTCATC ATCAGACTGG AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC
    AACACAAACA CAACAGCGTC CAGGCCTCAG TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330763.1
    CDS42..3986
    Translation

    Target ORF information:

    RefSeq Version XM_021475088.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475088.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAGT
    CCTGAAGAGT ACAGAGAAAA CACTTACATG TACAGGCCGG AGGAGCTCGA TGTCGACGCG
    AAGTTAAGTC GGCTCTGTGA GCAGGACAAA GTAGTGCGAA CTCAAGAAGA GAAACTGCAG
    CAGCTCCACA GAGAAAAGCA CACACTGGAG ACGGCGCTGC TCTCCGCCAG TCAGGAGATC
    GAGATGAGCT CTGAAAACCC AGCGGCGGTC CAGAGCGTGG TCCAGCAGAG GGACGTCCTG
    CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG CTGAGCCGCG TCAGCACTGA GCTGGAGCGA
    AGCTGGAGAG AATATGATCG ACTAGAGGCC GATGTTACAC TTGCCAAAAC TAACCTGCTG
    GAGCAGCTGG AGGCCCTCGG CAGCCCACAG ACGGAGCCGC CCAGTCAAAA GCATGTTCAG
    ATCCAGAAAG AGTTGTGGAG AATTCAGGAT GTGATGGAGG CGCTGAGCAA GAACAAACCC
    AAGAGAAACC CTGAACCCAG TTTTCCTGGT GCAAACCCTC TGTCAACCCT GCATAAAAGT
    GAAGGCCCGG ATTACAGGCT GTATAAGAGT GAGCCGGAGC TGACCACAGT AGCCGAAGTC
    GACGAGAGTA ACGGTGAAGA CAAATCAGAG CAGACAGCAG AGAAAGAGCC GCCCGGCGTC
    ACTAAAGGTA TAGTTTACCC GGTGGGAGTG GTCAGTCCCA GAACTAAATC ACCCATGCCC
    GAGTCCTCCA CCATCGCCTC CTACGTCACC TTGAGGAAGA CCAAAAAACC TGATCCCAGA
    ACATGTCAGC CGCAGGACCG TCCGCGCAGC GCTGTGGAGC AGATGAGCAG TGGTGCTGAA
    GTGGGCCGAA CACGCATGAG TGTGGAGGAG CAGATGGAGA GAATCAGACG ACACCAGCAG
    GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT GGCTCTCTAT CCCGCAGCCT GTCCTTCACT
    AAGGACAGCA CATACTACAC ACTGCAGGCA CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC
    GAGCAGGACG AGCGCAGAGA CATGACGCCT GTGGAGCGAG ACACAAAACA CACAAAACAC
    CCTGACGCAG AGGAAAACTG CAGAGACACT AAGGAGCAGC TGCCAGCACA CACCCACCTG
    GAGATTAAGG ACAAAGCATC TGTGACTCGC TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG
    AGTGTGAGTC CCAAACCCGT GCTGAAGCAG GACGAATCTG AGTGTAAACT GGAGTACGAC
    AGAAAGCTGG CCACTCAGAA CAGCCTGCAG ACGGCAGTGA TGGTGCGTGT GGGCACTGAG
    GAGGATGAAG AGGATGAAGA AGAGGAGCAG AAAGACTCAG TTCAGCAGCA GGAGGACTCT
    TATGAGCTCA GCAACAGCAA ACACAGCACA CTGATCTCAG CATCATGTGA CAGGAAGAAC
    ACATCATCAC CATCGTCATC ATCAGACTGG AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC
    AACACAAACA CAACAGCGTC CAGGCCTCAG TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142032
    Clone ID Related Accession (Same CDS sequence) XM_021475089.1
    Accession Version XM_021475089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT
    GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG
    AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT
    CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG
    GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG
    CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT
    AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG
    CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG
    AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG
    CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC
    TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC
    CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC
    GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC
    GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT
    AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG
    TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA
    TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG
    GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG
    GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG
    GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG
    CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT
    GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG
    ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT
    GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA
    AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG
    GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT
    GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA
    TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC
    ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330764.1
    CDS42..3983
    Translation

    Target ORF information:

    RefSeq Version XM_021475089.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CAGATGAGTC CTGAAGAGTA CAGAGAAAAC ACTTACATGT ACAGGCCGGA GGAGCTCGAT
    GTCGACGCGA AGTTAAGTCG GCTCTGTGAG CAGGACAAAG TAGTGCGAAC TCAAGAAGAG
    AAACTGCAGC AGCTCCACAG AGAAAAGCAC ACACTGGAGA CGGCGCTGCT CTCCGCCAGT
    CAGGAGATCG AGATGAGCTC TGAAAACCCA GCGGCGGTCC AGAGCGTGGT CCAGCAGAGG
    GACGTCCTGC AGAGTGGCCT GCTGAGCACC TGCAGAGAGC TGAGCCGCGT CAGCACTGAG
    CTGGAGCGAA GCTGGAGAGA ATATGATCGA CTAGAGGCCG ATGTTACACT TGCCAAAACT
    AACCTGCTGG AGCAGCTGGA GGCCCTCGGC AGCCCACAGA CGGAGCCGCC CAGTCAAAAG
    CATGTTCAGA TCCAGAAAGA GTTGTGGAGA ATTCAGGATG TGATGGAGGC GCTGAGCAAG
    AACAAACCCA AGAGAAACCC TGAACCCAGT TTTCCTGGTG CAAACCCTCT GTCAACCCTG
    CATAAAAGTG AAGACTCTGT AGACTCCTCG TGTGTTCCTC CTCGTCCTCC TCTGCCGTAC
    TCATATGAAG GAGGCGATCG ACCACCGCCC CTCCCCAACC CCGGTCAACC GCACACCACC
    CGCACAACAC CACACCGACC AGAGGACCGC AAAACTGCCC AACGCAACGG CACGCACACC
    GGCCCGGATT ACAGGCTGTA TAAGAGTGAG CCGGAGCTGA CCACAGTAGC CGAAGTCGAC
    GAGAGTAACG GTGAAGACAA ATCAGAGCAG ACAGCAGAGA AAGAGCCGCC CGGCGTCACT
    AAAGGTATAG TTTACCCGGT GGGAGTGGTC AGTCCCAGAA CTAAATCACC CATGCCCGAG
    TCCTCCACCA TCGCCTCCTA CGTCACCTTG AGGAAGACCA AAAAACCTGA TCCCAGAACA
    TGTCAGCCGC AGGACCGTCC GCGCAGCGCT GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG
    GGCCGAACAC GCATGAGTGT GGAGGAGCAG ATGGAGAGAA TCAGACGACA CCAGCAGGGG
    GCGCTGAGAG AGCGCAGGAG AGAAGATGGC TCTCTATCCC GCAGCCTGTC CTTCACTAAG
    GACAGCACAT ACTACACACT GCAGGCACGT AAGAAGAGTC CGGTGATCTC TCCAGACGAG
    CAGGACGAGC GCAGAGACAT GACGCCTGTG GAGCGAGACA CAAAACACAC AAAACACCCT
    GACGCAGAGG AAAACTGCAG AGACACTAAG GAGCAGCTGC CAGCACACAC CCACCTGGAG
    ATTAAGGACA AAGCATCTGT GACTCGCTCG GTCTCCATGA AAGAGTCTCT CCTGAAGAGT
    GTGAGTCCCA AACCCGTGCT GAAGCAGGAC GAATCTGAGT GTAAACTGGA GTACGACAGA
    AAGCTGGCCA CTCAGAACAG CCTGCAGACG GCAGTGATGG TGCGTGTGGG CACTGAGGAG
    GATGAAGAGG ATGAAGAAGA GGAGCAGAAA GACTCAGTTC AGCAGCAGGA GGACTCTTAT
    GAGCTCAGCA ACAGCAAACA CAGCACACTG ATCTCAGCAT CATGTGACAG GAAGAACACA
    TCATCACCAT CGTCATCATC AGACTGGAAG TCTCGCCCCC TCCTTCATCA GCTGCCCAAC
    ACAAACACAA CAGCGTCCAG GCCTCAGTTC AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142033
    Clone ID Related Accession (Same CDS sequence) XM_021475090.1
    Accession Version XM_021475090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC
    GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA
    CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG
    GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC
    GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG
    GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC
    CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT
    GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC
    AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT
    AAAAGTGAAG ACTCTGTAGA CTCCTCGTGT GTTCCTCCTC GTCCTCCTCT GCCGTACTCA
    TATGAAGGAG GCGATCGACC ACCGCCCCTC CCCAACCCCG GTCAACCGCA CACCACCCGC
    ACAACACCAC ACCGACCAGA GGACCGCAAA ACTGCCCAAC GCAACGGCAC GCACACCGGC
    CCGGATTACA GGCTGTATAA GAGTGAGCCG GAGCTGACCA CAGTAGCCGA AGTCGACGAG
    AGTAACGGTG AAGACAAATC AGAGCAGACA GCAGAGAAAG AGCCGCCCGG CGTCACTAAA
    GGTATAGTTT ACCCGGTGGG AGTGGTCAGT CCCAGAACTA AATCACCCAT GCCCGAGTCC
    TCCACCATCG CCTCCTACGT CACCTTGAGG AAGACCAAAA AACCTGATCC CAGAACATGT
    CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG GAGCAGATGA GCAGTGGTGC TGAAGTGGGC
    CGAACACGCA TGAGTGTGGA GGAGCAGATG GAGAGAATCA GACGACACCA GCAGGGGGCG
    CTGAGAGAGC GCAGGAGAGA AGATGGCTCT CTATCCCGCA GCCTGTCCTT CACTAAGGAC
    AGCACATACT ACACACTGCA GGCACGTAAG AAGAGTCCGG TGATCTCTCC AGACGAGCAG
    GACGAGCGCA GAGACATGAC GCCTGTGGAG CGAGACACAA AACACACAAA ACACCCTGAC
    GCAGAGGAAA ACTGCAGAGA CACTAAGGAG CAGCTGCCAG CACACACCCA CCTGGAGATT
    AAGGACAAAG CATCTGTGAC TCGCTCGGTC TCCATGAAAG AGTCTCTCCT GAAGAGTGTG
    AGTCCCAAAC CCGTGCTGAA GCAGGACGAA TCTGAGTGTA AACTGGAGTA CGACAGAAAG
    CTGGCCACTC AGAACAGCCT GCAGACGGCA GTGATGGTGC GTGTGGGCAC TGAGGAGGAT
    GAAGAGGATG AAGAAGAGGA GCAGAAAGAC TCAGTTCAGC AGCAGGAGGA CTCTTATGAG
    CTCAGCAACA GCAAACACAG CACACTGATC TCAGCATCAT GTGACAGGAA GAACACATCA
    TCACCATCGT CATCATCAGA CTGGAAGTCT CGCCCCCTCC TTCATCAGCT GCCCAACACA
    AACACAACAG CGTCCAGGCC TCAGTTCAAC AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330765.1
    CDS42..3980
    Translation

    Target ORF information:

    RefSeq Version XM_021475090.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC
    GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA
    CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG
    GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC
    GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG
    GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC
    CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT
    GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC
    AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT
    AAAAGTGAAG ACTCTGTAGA CTCCTCGTGT GTTCCTCCTC GTCCTCCTCT GCCGTACTCA
    TATGAAGGAG GCGATCGACC ACCGCCCCTC CCCAACCCCG GTCAACCGCA CACCACCCGC
    ACAACACCAC ACCGACCAGA GGACCGCAAA ACTGCCCAAC GCAACGGCAC GCACACCGGC
    CCGGATTACA GGCTGTATAA GAGTGAGCCG GAGCTGACCA CAGTAGCCGA AGTCGACGAG
    AGTAACGGTG AAGACAAATC AGAGCAGACA GCAGAGAAAG AGCCGCCCGG CGTCACTAAA
    GGTATAGTTT ACCCGGTGGG AGTGGTCAGT CCCAGAACTA AATCACCCAT GCCCGAGTCC
    TCCACCATCG CCTCCTACGT CACCTTGAGG AAGACCAAAA AACCTGATCC CAGAACATGT
    CAGCCGCAGG ACCGTCCGCG CAGCGCTGTG GAGCAGATGA GCAGTGGTGC TGAAGTGGGC
    CGAACACGCA TGAGTGTGGA GGAGCAGATG GAGAGAATCA GACGACACCA GCAGGGGGCG
    CTGAGAGAGC GCAGGAGAGA AGATGGCTCT CTATCCCGCA GCCTGTCCTT CACTAAGGAC
    AGCACATACT ACACACTGCA GGCACGTAAG AAGAGTCCGG TGATCTCTCC AGACGAGCAG
    GACGAGCGCA GAGACATGAC GCCTGTGGAG CGAGACACAA AACACACAAA ACACCCTGAC
    GCAGAGGAAA ACTGCAGAGA CACTAAGGAG CAGCTGCCAG CACACACCCA CCTGGAGATT
    AAGGACAAAG CATCTGTGAC TCGCTCGGTC TCCATGAAAG AGTCTCTCCT GAAGAGTGTG
    AGTCCCAAAC CCGTGCTGAA GCAGGACGAA TCTGAGTGTA AACTGGAGTA CGACAGAAAG
    CTGGCCACTC AGAACAGCCT GCAGACGGCA GTGATGGTGC GTGTGGGCAC TGAGGAGGAT
    GAAGAGGATG AAGAAGAGGA GCAGAAAGAC TCAGTTCAGC AGCAGGAGGA CTCTTATGAG
    CTCAGCAACA GCAAACACAG CACACTGATC TCAGCATCAT GTGACAGGAA GAACACATCA
    TCACCATCGT CATCATCAGA CTGGAAGTCT CGCCCCCTCC TTCATCAGCT GCCCAACACA
    AACACAACAG CGTCCAGGCC TCAGTTCAAC AGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142034
    Clone ID Related Accession (Same CDS sequence) XM_021475091.1
    Accession Version XM_021475091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3771bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC
    GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA
    CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG
    GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC
    GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG
    GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC
    CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT
    GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC
    AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT
    AAAAGTGAAG GCCCGGATTA CAGGCTGTAT AAGAGTGAGC CGGAGCTGAC CACAGTAGCC
    GAAGTCGACG AGAGTAACGG TGAAGACAAA TCAGAGCAGA CAGCAGAGAA AGAGCCGCCC
    GGCGTCACTA AAGGTATAGT TTACCCGGTG GGAGTGGTCA GTCCCAGAAC TAAATCACCC
    ATGCCCGAGT CCTCCACCAT CGCCTCCTAC GTCACCTTGA GGAAGACCAA AAAACCTGAT
    CCCAGAACAT GTCAGCCGCA GGACCGTCCG CGCAGCGCTG TGGAGCAGAT GAGCAGTGGT
    GCTGAAGTGG GCCGAACACG CATGAGTGTG GAGGAGCAGA TGGAGAGAAT CAGACGACAC
    CAGCAGGGGG CGCTGAGAGA GCGCAGGAGA GAAGATGGCT CTCTATCCCG CAGCCTGTCC
    TTCACTAAGG ACAGCACATA CTACACACTG CAGGCACGTA AGAAGAGTCC GGTGATCTCT
    CCAGACGAGC AGGACGAGCG CAGAGACATG ACGCCTGTGG AGCGAGACAC AAAACACACA
    AAACACCCTG ACGCAGAGGA AAACTGCAGA GACACTAAGG AGCAGCTGCC AGCACACACC
    CACCTGGAGA TTAAGGACAA AGCATCTGTG ACTCGCTCGG TCTCCATGAA AGAGTCTCTC
    CTGAAGAGTG TGAGTCCCAA ACCCGTGCTG AAGCAGGACG AATCTGAGTG TAAACTGGAG
    TACGACAGAA AGCTGGCCAC TCAGAACAGC CTGCAGACGG CAGTGATGGT GCGTGTGGGC
    ACTGAGGAGG ATGAAGAGGA TGAAGAAGAG GAGCAGAAAG ACTCAGTTCA GCAGCAGGAG
    GACTCTTATG AGCTCAGCAA CAGCAAACAC AGCACACTGA TCTCAGCATC ATGTGACAGG
    AAGAACACAT CATCACCATC GTCATCATCA GACTGGAAGT CTCGCCCCCT CCTTCATCAG
    CTGCCCAACA CAAACACAAC AGCGTCCAGG CCTCAGTTCA ACAGCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330766.1
    CDS42..3812
    Translation

    Target ORF information:

    RefSeq Version XM_021475091.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    ATGAGTCCTG AAGAGTACAG AGAAAACACT TACATGTACA GGCCGGAGGA GCTCGATGTC
    GACGCGAAGT TAAGTCGGCT CTGTGAGCAG GACAAAGTAG TGCGAACTCA AGAAGAGAAA
    CTGCAGCAGC TCCACAGAGA AAAGCACACA CTGGAGACGG CGCTGCTCTC CGCCAGTCAG
    GAGATCGAGA TGAGCTCTGA AAACCCAGCG GCGGTCCAGA GCGTGGTCCA GCAGAGGGAC
    GTCCTGCAGA GTGGCCTGCT GAGCACCTGC AGAGAGCTGA GCCGCGTCAG CACTGAGCTG
    GAGCGAAGCT GGAGAGAATA TGATCGACTA GAGGCCGATG TTACACTTGC CAAAACTAAC
    CTGCTGGAGC AGCTGGAGGC CCTCGGCAGC CCACAGACGG AGCCGCCCAG TCAAAAGCAT
    GTTCAGATCC AGAAAGAGTT GTGGAGAATT CAGGATGTGA TGGAGGCGCT GAGCAAGAAC
    AAACCCAAGA GAAACCCTGA ACCCAGTTTT CCTGGTGCAA ACCCTCTGTC AACCCTGCAT
    AAAAGTGAAG GCCCGGATTA CAGGCTGTAT AAGAGTGAGC CGGAGCTGAC CACAGTAGCC
    GAAGTCGACG AGAGTAACGG TGAAGACAAA TCAGAGCAGA CAGCAGAGAA AGAGCCGCCC
    GGCGTCACTA AAGGTATAGT TTACCCGGTG GGAGTGGTCA GTCCCAGAAC TAAATCACCC
    ATGCCCGAGT CCTCCACCAT CGCCTCCTAC GTCACCTTGA GGAAGACCAA AAAACCTGAT
    CCCAGAACAT GTCAGCCGCA GGACCGTCCG CGCAGCGCTG TGGAGCAGAT GAGCAGTGGT
    GCTGAAGTGG GCCGAACACG CATGAGTGTG GAGGAGCAGA TGGAGAGAAT CAGACGACAC
    CAGCAGGGGG CGCTGAGAGA GCGCAGGAGA GAAGATGGCT CTCTATCCCG CAGCCTGTCC
    TTCACTAAGG ACAGCACATA CTACACACTG CAGGCACGTA AGAAGAGTCC GGTGATCTCT
    CCAGACGAGC AGGACGAGCG CAGAGACATG ACGCCTGTGG AGCGAGACAC AAAACACACA
    AAACACCCTG ACGCAGAGGA AAACTGCAGA GACACTAAGG AGCAGCTGCC AGCACACACC
    CACCTGGAGA TTAAGGACAA AGCATCTGTG ACTCGCTCGG TCTCCATGAA AGAGTCTCTC
    CTGAAGAGTG TGAGTCCCAA ACCCGTGCTG AAGCAGGACG AATCTGAGTG TAAACTGGAG
    TACGACAGAA AGCTGGCCAC TCAGAACAGC CTGCAGACGG CAGTGATGGT GCGTGTGGGC
    ACTGAGGAGG ATGAAGAGGA TGAAGAAGAG GAGCAGAAAG ACTCAGTTCA GCAGCAGGAG
    GACTCTTATG AGCTCAGCAA CAGCAAACAC AGCACACTGA TCTCAGCATC ATGTGACAGG
    AAGAACACAT CATCACCATC GTCATCATCA GACTGGAAGT CTCGCCCCCT CCTTCATCAG
    CTGCCCAACA CAAACACAAC AGCGTCCAGG CCTCAGTTCA ACAGCGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142035
    Clone ID Related Accession (Same CDS sequence) XM_021475093.1
    Accession Version XM_021475093.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGT GAACAGTGTG
    TCGACTCCTC CTCAATCTCC AAGCTCCTCC TCCTCTCCTC CGCCTCCACC CTCCCCTCCA
    CAGCTGACGG ACGGGTCACA CTTCATGTGT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330768.1
    CDS42..4217
    Translation

    Target ORF information:

    RefSeq Version XM_021475093.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475093.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC ACCTGAATTG TGGGAATCTT GTCCATCCTG TTTTAAGCAG ACCAGCGACC
    AAAGCACCAG AGGCGGAGAA GAAGGAGCGT CCCACCAGCA CCATGAGCGA AGCCTCAAAC
    TACACCGGCG GCTCCGACTA CACCACACAT CCCAGCAGCC CCACAACCAG ACCATCCCGG
    TCCTCAAAGA AAGTGCACAA CTTTGGGAAA AGGTCCAACT CCATCAAGAG GAACCCCAAT
    GCACCTGTGA TCAAAAACGG CTGGCTTCAC AAACAGGACA GCACTGGGAT GAAGATGTGG
    AAGAAGAGAT GGTTCGTTCT GTCTGATATG TGCCTCTTCT ACTACAGAGA TGAGAAGGAA
    GAGGGGATTC TGGGAAGCAT CCTCCTGCCT AGCTTTCACA TCTCCATGCT GTCCGTGGAC
    GACCATATTA CCCGGAAATA CGCCTTCAAG GCGACGCATC CAAACATGCG GACGTATTAT
    TTCAGCACAG ACACAGCCAA AGATATGGAG TCCTGGATGA AAGTGATGTC GGATGCGGCG
    ATGGCTCACA GCGAGCCAAT CAGAAGGCTT GAGAAGGTAA AGCTGGACTC TCGCGTTCCT
    CAGGAGATGA ACAACATGCT GAACCACCGC GTCTTGACAC GACCCGAGAT CCAAAACAAC
    GAGAGGAACC GCGAGACGCT CCGCCAAGAG GACAAGAAGA GCAAACCATC AGAGAAACCT
    CAGAAAGACA AAGACAGAGT CAGCATGCAG AAAGACGGAG AGCGCTTCAG TGAAAACTAC
    ACTCACCGGC AGAAAGACGG CGAGCGCTAC ATGCTGCAGA AAGACCTGGA GAAATTTGTC
    CTGCAGAAAG ACGGAGAGAA GTACGCCGTG CAGAAAGAAG GCGACAAATA TCTCCTGCAG
    AAAGACGGAC ACAAATACAC AGTGCACAAA GACGGAGACA AATACACGCT TCAGAGAGAA
    CTCGAGATGT ACAATCCACC AAAAGACGCG GAGAAGATCC CGCAGAAAGA TGCAAGGTTT
    AGTCTGGTGC AGAAGGAGCT GGAGAAACCC GTCCCGCCAC CGCGATCTGA GGAAAAATAC
    GGCTTTCAGA AAGAGGGCAC CATTGAGCGT CCGCTAACTA AAATAAATAG CATTAAACTG
    CAGCCGGCTC AAGCAGCCGC CATCGCTGCA GCCGTTTCAT CCTCTAGGAT GATGCAGAAA
    CAGGTTAATG GCTCCGGGGA ACGAGCGGGA GACAGAAGTC CTGGAGATTT GAGTACTGTG
    AGGACGAGCG TCCCGCCACC AACGCAGCCG GCTCCAGAAC CAGAGCGAAG CCTCAGCAGA
    ACCAGCTCAA TGCAGCAGCT GGAGCAGTGG GTTCGCACAC ACCGCACACG AGCTCCAGAC
    GACGACACCA GGAGCATTAC GTCCTACCAG ACCCTGCCAA GGAACATGCC GAGCCACCGG
    GCGCAGATTG TCCCTCGCTA CCCTGAGGGC TACCGCACTC TGCCCCGAAA CATGCTGCGT
    CCGGACAGCA TCTGCAGTGT AGCAGGATCA GTGTACGACC GAGCGCTTCA GCCCAGCACG
    CAGGAAAAGC GGCGCTCCAT CCGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG
    CGGCAGGCGC ACAGTCGGCT TGGGTACGGG ACGCTGCCCA GTCCCAAAAC TATGGGGCAG
    ATCGCGGAGT CCATCCCCAC GTCCCCATCC CATGGCTCTC TGGCCGGATA TCACACCTTC
    TCCCCGAACA GACCGCTCAA CCCTGACTCG CGCTCGGAGC TGTCTTCGCC TGTGTTTCGT
    GGAGACGTGA CCATCGAGCG ACGCCACCGA CCACACCTGT CCAAGTACTC CTATCCTGCG
    GAACGCAGAG CTGCTCCTCC CGCACAGAAC ATCACTGCAC AGTCTCTTCA AGGCAAAACG
    CCAGAGGAAC TGACCCTGCT GCTCATCAAA CTGCGGCGGC AGCAGGCCGA GCTCAACAGC
    CTCCGTGAGC ACACACTCGC GCAGCTCATG CAGCTGAACC TCGACGCCAC CAACCCAAAG
    AGTGAAATCC TCTCTCATCA CCTGCAGAGG AATCTCATGT ACTTGGACAG TCAGATGAAG
    GAGAACGAAC CCATAATCTT CATGATTCAC ACTATGATTG AGAACTCGGC GCCCCGGCCG
    CAGCTCTACC AGCAAATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGT GAACAGTGTG
    TCGACTCCTC CTCAATCTCC AAGCTCCTCC TCCTCTCCTC CGCCTCCACC CTCCCCTCCA
    CAGCTGACGG ACGGGTCACA CTTCATGTGT GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45107
    Clone ID Related Accession (Same CDS sequence) XM_009299998.2
    Accession Version XM_009299998.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298273.1
    CDS44..4198
    Translation

    Target ORF information:

    RefSeq Version XM_009299998.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299998.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG CATCATGTGA CAGGAAGAAC ACATCATCAC CATCGTCATC ATCAGACTGG
    AAGTCTCGCC CCCTCCTTCA TCAGCTGCCC AACACAAACA CAACAGCGTC CAGGCCTCAG
    TTCAACAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45108
    Clone ID Related Accession (Same CDS sequence) XM_009299999.2 , XM_009299999.3
    Accession Version XM_009299999.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT
    CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298274.1
    CDS44..4183
    Translation

    Target ORF information:

    RefSeq Version XM_009299999.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299999.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT
    CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45108
    Clone ID Related Accession (Same CDS sequence) XM_009299999.2 , XM_009299999.3
    Accession Version XM_009299999.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT
    CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298274.1
    CDS42..4181
    Translation

    Target ORF information:

    RefSeq Version XM_009299999.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299999.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGA CTCTGTAGAC
    TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG CCGTACTCAT ATGAAGGAGG CGATCGACCA
    CCGCCCCTCC CCAACCCCGG TCAACCGCAC ACCACCCGCA CAACACCACA CCGACCAGAG
    GACCGCAAAA CTGCCCAACG CAACGGCACG CACACCGGCC CGGATTACAG GCTGTATAAG
    AGTGAGCCGG AGCTGACCAC AGTAGCCGAA GTCGACGAGA GTAACGGTGA AGACAAATCA
    GAGCAGACAG CAGAGAAAGA GCCGCCCGGC GTCACTAAAG GTATAGTTTA CCCGGTGGGA
    GTGGTCAGTC CCAGAACTAA ATCACCCATG CCCGAGTCCT CCACCATCGC CTCCTACGTC
    ACCTTGAGGA AGACCAAAAA ACCTGATCCC AGAACATGTC AGCCGCAGGA CCGTCCGCGC
    AGCGCTGTGG AGCAGATGAG CAGTGGTGCT GAAGTGGGCC GAACACGCAT GAGTGTGGAG
    GAGCAGATGG AGAGAATCAG ACGACACCAG CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA
    GATGGCTCTC TATCCCGCAG CCTGTCCTTC ACTAAGGACA GCACATACTA CACACTGCAG
    GCACGTAAGA AGAGTCCGGT GATCTCTCCA GACGAGCAGG ACGAGCGCAG AGACATGACG
    CCTGTGGAGC GAGACACAAA ACACACAAAA CACCCTGACG CAGAGGAAAA CTGCAGAGAC
    ACTAAGGAGC AGCTGCCAGC ACACACCCAC CTGGAGATTA AGGACAAAGC ATCTGTGACT
    CGCTCGGTCT CCATGAAAGA GTCTCTCCTG AAGAGTGTGA GTCCCAAACC CGTGCTGAAG
    CAGGACGAAT CTGAGTGTAA ACTGGAGTAC GACAGAAAGC TGGCCACTCA GAACAGCCTG
    CAGACGGCAG TGATGGTGCG TGTGGGCACT GAGGAGGATG AAGAGGATGA AGAAGAGGAG
    CAGAAAGACT CAGTTCAGCA GCAGGAGGAC TCTTATGAGC TCAGCAACAG CAAACACAGC
    ACACTGATCT CAGTGAACAG TGTGTCGACT CCTCCTCAAT CTCCAAGCTC CTCCTCCTCT
    CCTCCGCCTC CACCCTCCCC TCCACAGCTG ACGGACGGGT CACACTTCAT GTGTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45109
    Clone ID Related Accession (Same CDS sequence) XM_009300000.2
    Accession Version XM_009300000.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGCAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298275.1
    CDS44..4120
    Translation

    Target ORF information:

    RefSeq Version XM_009300000.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGCAGAT GAGTCCTGAA GAGTACAGAG AAAACACTTA CATGTACAGG
    CCGGAGGAGC TCGATGTCGA CGCGAAGTTA AGTCGGCTCT GTGAGCAGGA CAAAGTAGTG
    CGAACTCAAG AAGAGAAACT GCAGCAGCTC CACAGAGAAA AGCACACACT GGAGACGGCG
    CTGCTCTCCG CCAGTCAGGA GATCGAGATG AGCTCTGAAA ACCCAGCGGC GGTCCAGAGC
    GTGGTCCAGC AGAGGGACGT CCTGCAGAGT GGCCTGCTGA GCACCTGCAG AGAGCTGAGC
    CGCGTCAGCA CTGAGCTGGA GCGAAGCTGG AGAGAATATG ATCGACTAGA GGCCGATGTT
    ACACTTGCCA AAACTAACCT GCTGGAGCAG CTGGAGGCCC TCGGCAGCCC ACAGACGGAG
    CCGCCCAGTC AAAAGCATGT TCAGATCCAG AAAGAGTTGT GGAGAATTCA GGATGTGATG
    GAGGCGCTGA GCAAGAACAA ACCCAAGAGA AACCCTGAAC CCAGTTTTCC TGGTGCAAAC
    CCTCTGTCAA CCCTGCATAA AAGTGAAGAC TCTGTAGACT CCTCGTGTGT TCCTCCTCGT
    CCTCCTCTGC CGTACTCATA TGAAGGAGGC GATCGACCAC CGCCCCTCCC CAACCCCGGT
    CAACCGCACA CCACCCGCAC AACACCACAC CGACCAGAGG ACCGCAAAAC TGCCCAACGC
    AACGGCACGC ACACCGGCCC GGATTACAGG CTGTATAAGA GTGAGCCGGA GCTGACCACA
    GTAGCCGAAG TCGACGAGAG TAACGGTGAA GACAAATCAG AGCAGACAGC AGAGAAAGAG
    CCGCCCGGCG TCACTAAAGG TATAGTTTAC CCGGTGGGAG TGGTCAGTCC CAGAACTAAA
    TCACCCATGC CCGAGTCCTC CACCATCGCC TCCTACGTCA CCTTGAGGAA GACCAAAAAA
    CCTGATCCCA GAACATGTCA GCCGCAGGAC CGTCCGCGCA GCGCTGTGGA GCAGATGAGC
    AGTGGTGCTG AAGTGGGCCG AACACGCATG AGTGTGGAGG AGCAGATGGA GAGAATCAGA
    CGACACCAGC AGGGGGCGCT GAGAGAGCGC AGGAGAGAAG ATGGCTCTCT ATCCCGCAGC
    CTGTCCTTCA CTAAGGACAG CACATACTAC ACACTGCAGG CACGTAAGAA GAGTCCGGTG
    ATCTCTCCAG ACGAGCAGGA CGAGCGCAGA GACATGACGC CTGTGGAGCG AGACACAAAA
    CACACAAAAC ACCCTGACGC AGAGGAAAAC TGCAGAGACA CTAAGGAGCA GCTGCCAGCA
    CACACCCACC TGGAGATTAA GGACAAAGCA TCTGTGACTC GCTCGGTCTC CATGAAAGAG
    TCTCTCCTGA AGAGTGTGAG TCCCAAACCC GTGCTGAAGC AGGACGAATC TGAGTGTAAA
    CTGGAGTACG ACAGAAAGCT GGCCACTCAG AACAGCCTGC AGACGGCAGT GATGGTGCGT
    GTGGGCACTG AGGAGGATGA AGAGGATGAA GAAGAGGAGC AGAAAGACTC AGTTCAGCAG
    CAGGAGGACT CTTATGAGCT CAGCAACAGC AAACACAGCA CACTGATCTC AGCATCATGT
    GACAGGAAGA ACACATCATC ACCATCGTCA TCATCAGACT GGAAGTCTCG CCCCCTCCTT
    CATCAGCTGC CCAACACAAA CACAACAGCG TCCAGGCCTC AGTTCAACAG CGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45110
    Clone ID Related Accession (Same CDS sequence) XM_009300001.2
    Accession Version XM_009300001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC
    AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT
    CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298276.1
    CDS44..4117
    Translation

    Target ORF information:

    RefSeq Version XM_009300001.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGACTCT GTAGACTCCT CGTGTGTTCC TCCTCGTCCT
    CCTCTGCCGT ACTCATATGA AGGAGGCGAT CGACCACCGC CCCTCCCCAA CCCCGGTCAA
    CCGCACACCA CCCGCACAAC ACCACACCGA CCAGAGGACC GCAAAACTGC CCAACGCAAC
    GGCACGCACA CCGGCCCGGA TTACAGGCTG TATAAGAGTG AGCCGGAGCT GACCACAGTA
    GCCGAAGTCG ACGAGAGTAA CGGTGAAGAC AAATCAGAGC AGACAGCAGA GAAAGAGCCG
    CCCGGCGTCA CTAAAGGTAT AGTTTACCCG GTGGGAGTGG TCAGTCCCAG AACTAAATCA
    CCCATGCCCG AGTCCTCCAC CATCGCCTCC TACGTCACCT TGAGGAAGAC CAAAAAACCT
    GATCCCAGAA CATGTCAGCC GCAGGACCGT CCGCGCAGCG CTGTGGAGCA GATGAGCAGT
    GGTGCTGAAG TGGGCCGAAC ACGCATGAGT GTGGAGGAGC AGATGGAGAG AATCAGACGA
    CACCAGCAGG GGGCGCTGAG AGAGCGCAGG AGAGAAGATG GCTCTCTATC CCGCAGCCTG
    TCCTTCACTA AGGACAGCAC ATACTACACA CTGCAGGCAC GTAAGAAGAG TCCGGTGATC
    TCTCCAGACG AGCAGGACGA GCGCAGAGAC ATGACGCCTG TGGAGCGAGA CACAAAACAC
    ACAAAACACC CTGACGCAGA GGAAAACTGC AGAGACACTA AGGAGCAGCT GCCAGCACAC
    ACCCACCTGG AGATTAAGGA CAAAGCATCT GTGACTCGCT CGGTCTCCAT GAAAGAGTCT
    CTCCTGAAGA GTGTGAGTCC CAAACCCGTG CTGAAGCAGG ACGAATCTGA GTGTAAACTG
    GAGTACGACA GAAAGCTGGC CACTCAGAAC AGCCTGCAGA CGGCAGTGAT GGTGCGTGTG
    GGCACTGAGG AGGATGAAGA GGATGAAGAA GAGGAGCAGA AAGACTCAGT TCAGCAGCAG
    GAGGACTCTT ATGAGCTCAG CAACAGCAAA CACAGCACAC TGATCTCAGC ATCATGTGAC
    AGGAAGAACA CATCATCACC ATCGTCATCA TCAGACTGGA AGTCTCGCCC CCTCCTTCAT
    CAGCTGCCCA ACACAAACAC AACAGCGTCC AGGCCTCAGT TCAACAGCGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45111
    Clone ID Related Accession (Same CDS sequence) XM_009300002.2
    Accession Version XM_009300002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGG CCCGGATTAC
    AGGCTGTATA AGAGTGAGCC GGAGCTGACC ACAGTAGCCG AAGTCGACGA GAGTAACGGT
    GAAGACAAAT CAGAGCAGAC AGCAGAGAAA GAGCCGCCCG GCGTCACTAA AGGTATAGTT
    TACCCGGTGG GAGTGGTCAG TCCCAGAACT AAATCACCCA TGCCCGAGTC CTCCACCATC
    GCCTCCTACG TCACCTTGAG GAAGACCAAA AAACCTGATC CCAGAACATG TCAGCCGCAG
    GACCGTCCGC GCAGCGCTGT GGAGCAGATG AGCAGTGGTG CTGAAGTGGG CCGAACACGC
    ATGAGTGTGG AGGAGCAGAT GGAGAGAATC AGACGACACC AGCAGGGGGC GCTGAGAGAG
    CGCAGGAGAG AAGATGGCTC TCTATCCCGC AGCCTGTCCT TCACTAAGGA CAGCACATAC
    TACACACTGC AGGCACGTAA GAAGAGTCCG GTGATCTCTC CAGACGAGCA GGACGAGCGC
    AGAGACATGA CGCCTGTGGA GCGAGACACA AAACACACAA AACACCCTGA CGCAGAGGAA
    AACTGCAGAG ACACTAAGGA GCAGCTGCCA GCACACACCC ACCTGGAGAT TAAGGACAAA
    GCATCTGTGA CTCGCTCGGT CTCCATGAAA GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA
    CCCGTGCTGA AGCAGGACGA ATCTGAGTGT AAACTGGAGT ACGACAGAAA GCTGGCCACT
    CAGAACAGCC TGCAGACGGC AGTGATGGTG CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT
    GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG CAGCAGGAGG ACTCTTATGA GCTCAGCAAC
    AGCAAACACA GCACACTGAT CTCAGCATCA TGTGACAGGA AGAACACATC ATCACCATCG
    TCATCATCAG ACTGGAAGTC TCGCCCCCTC CTTCATCAGC TGCCCAACAC AAACACAACA
    GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298277.1
    CDS43..4032
    Translation

    Target ORF information:

    RefSeq Version XM_009300002.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAACAGA TGAGTCCTGA AGAGTACAGA
    GAAAACACTT ACATGTACAG GCCGGAGGAG CTCGATGTCG ACGCGAAGTT AAGTCGGCTC
    TGTGAGCAGG ACAAAGTAGT GCGAACTCAA GAAGAGAAAC TGCAGCAGCT CCACAGAGAA
    AAGCACACAC TGGAGACGGC GCTGCTCTCC GCCAGTCAGG AGATCGAGAT GAGCTCTGAA
    AACCCAGCGG CGGTCCAGAG CGTGGTCCAG CAGAGGGACG TCCTGCAGAG TGGCCTGCTG
    AGCACCTGCA GAGAGCTGAG CCGCGTCAGC ACTGAGCTGG AGCGAAGCTG GAGAGAATAT
    GATCGACTAG AGGCCGATGT TACACTTGCC AAAACTAACC TGCTGGAGCA GCTGGAGGCC
    CTCGGCAGCC CACAGACGGA GCCGCCCAGT CAAAAGCATG TTCAGATCCA GAAAGAGTTG
    TGGAGAATTC AGGATGTGAT GGAGGCGCTG AGCAAGAACA AACCCAAGAG AAACCCTGAA
    CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA ACCCTGCATA AAAGTGAAGG CCCGGATTAC
    AGGCTGTATA AGAGTGAGCC GGAGCTGACC ACAGTAGCCG AAGTCGACGA GAGTAACGGT
    GAAGACAAAT CAGAGCAGAC AGCAGAGAAA GAGCCGCCCG GCGTCACTAA AGGTATAGTT
    TACCCGGTGG GAGTGGTCAG TCCCAGAACT AAATCACCCA TGCCCGAGTC CTCCACCATC
    GCCTCCTACG TCACCTTGAG GAAGACCAAA AAACCTGATC CCAGAACATG TCAGCCGCAG
    GACCGTCCGC GCAGCGCTGT GGAGCAGATG AGCAGTGGTG CTGAAGTGGG CCGAACACGC
    ATGAGTGTGG AGGAGCAGAT GGAGAGAATC AGACGACACC AGCAGGGGGC GCTGAGAGAG
    CGCAGGAGAG AAGATGGCTC TCTATCCCGC AGCCTGTCCT TCACTAAGGA CAGCACATAC
    TACACACTGC AGGCACGTAA GAAGAGTCCG GTGATCTCTC CAGACGAGCA GGACGAGCGC
    AGAGACATGA CGCCTGTGGA GCGAGACACA AAACACACAA AACACCCTGA CGCAGAGGAA
    AACTGCAGAG ACACTAAGGA GCAGCTGCCA GCACACACCC ACCTGGAGAT TAAGGACAAA
    GCATCTGTGA CTCGCTCGGT CTCCATGAAA GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA
    CCCGTGCTGA AGCAGGACGA ATCTGAGTGT AAACTGGAGT ACGACAGAAA GCTGGCCACT
    CAGAACAGCC TGCAGACGGC AGTGATGGTG CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT
    GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG CAGCAGGAGG ACTCTTATGA GCTCAGCAAC
    AGCAAACACA GCACACTGAT CTCAGCATCA TGTGACAGGA AGAACACATC ATCACCATCG
    TCATCATCAG ACTGGAAGTC TCGCCCCCTC CTTCATCAGC TGCCCAACAC AAACACAACA
    GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45112
    Clone ID Related Accession (Same CDS sequence) XM_009300003.2 , XM_009300003.1
    Accession Version XM_009300003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA
    GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC
    TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA
    TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC
    CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG
    ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG
    AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC
    GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG
    TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT
    GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC
    GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA
    AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG
    AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA
    AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA
    TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC
    CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG
    AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA
    AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA
    AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT
    AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG
    CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT
    ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC
    AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT
    CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC
    CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG
    CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC
    AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG
    CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG
    GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC
    ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG
    TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT
    CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC
    AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC
    AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC
    CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG
    ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC
    CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298278.1
    CDS74..4036
    Translation

    Target ORF information:

    RefSeq Version XM_009300003.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA
    GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC
    TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA
    TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC
    CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG
    ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG
    AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC
    GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG
    TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT
    GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC
    GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA
    AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG
    AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA
    AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA
    TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC
    CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG
    AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA
    AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA
    AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT
    AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG
    CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT
    ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC
    AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT
    CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC
    CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG
    CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC
    AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG
    CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG
    GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC
    ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG
    TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT
    CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC
    AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC
    AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC
    CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG
    ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC
    CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45112
    Clone ID Related Accession (Same CDS sequence) XM_009300003.2 , XM_009300003.1
    Accession Version XM_009300003.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA
    GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC
    TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA
    TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC
    CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG
    ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG
    AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC
    GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG
    TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT
    GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC
    GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA
    AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG
    AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA
    AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA
    TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC
    CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG
    AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA
    AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA
    AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT
    AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG
    CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT
    ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC
    AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT
    CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC
    CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG
    CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC
    AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG
    CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG
    GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC
    ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG
    TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT
    CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC
    AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC
    AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC
    CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG
    ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC
    CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298278.1
    CDS33..3995
    Translation

    Target ORF information:

    RefSeq Version XM_009300003.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGAAGT TCTGCACCAC CTCTCGCTCC AGCCACAATG AGCGAAAGGT GACCTGCAAG 
    CATCCCGTCA GCGGCGTCCC GTCACAAGAC AACTGCATCT TTGTGGTCAA CGAACAACCA
    GCGACCAAAG CACCAGAGGC GGAGAAGAAG GAGCGTCCCA CCAGCACCAT GAGCGAAGCC
    TCAAACTACA CCGGCGGCTC CGACTACACC ACACATCCCA GCAGCCCCAC AACCAGACCA
    TCCCGGTCCT CAAAGAAAGT GCACAACTTT GGGAAAAGGT CCAACTCCAT CAAGAGGAAC
    CCCAATGCAC CTGTGATCAA AAACGGCTGG CTTCACAAAC AGGACAGCAC TGGGATGAAG
    ATGTGGAAGA AGAGATGGTT CGTTCTGTCT GATATGTGCC TCTTCTACTA CAGAGATGAG
    AAGGAAGAGG GGATTCTGGG AAGCATCCTC CTGCCTAGCT TTCACATCTC CATGCTGTCC
    GTGGACGACC ATATTACCCG GAAATACGCC TTCAAGGCGA CGCATCCAAA CATGCGGACG
    TATTATTTCA GCACAGACAC AGCCAAAGAT ATGGAGTCCT GGATGAAAGT GATGTCGGAT
    GCGGCGATGG CTCACAGCGA GCCAATCAGA AGGCTTGAGA AGGTAAAGCT GGACTCTCGC
    GTTCCTCAGG AGATGAACAA CATGCTGAAC CACCGCGTCT TGACACGACC CGAGATCCAA
    AACAACGAGA GGAACCGCGA GACGCTCCGC CAAGAGGACA AGAAGAGCAA ACCATCAGAG
    AAACCTCAGA AAGACAAAGA CAGAGTCAGC ATGCAGAAAG ACGGAGAGCG CTTCAGTGAA
    AACTACACTC ACCGGCAGAA AGACGGCGAG CGCTACATGC TGCAGAAAGA CCTGGAGAAA
    TTTGTCCTGC AGAAAGACGG AGAGAAGTAC GCCGTGCAGA AAGAAGGCGA CAAATATCTC
    CTGCAGAAAG ACGGACACAA ATACACAGTG CACAAAGACG GAGACAAATA CACGCTTCAG
    AGAGAACTCG AGATGTACAA TCCACCAAAA GACGCGGAGA AGATCCCGCA GAAAGATGCA
    AGGTTTAGTC TGGTGCAGAA GGAGCTGGAG AAACCCGTCC CGCCACCGCG ATCTGAGGAA
    AAATACGGCT TTCAGAAAGA GGGCACCATT GAGCGTCCGC TAACTAAAAT AAATAGCATT
    AAACTGCAGC CGGCTCAAGC AGCCGCCATC GCTGCAGCCG TTTCATCCTC TAGGATGATG
    CAGAAACAGG TTAATGGCTC CGGGGAACGA GCGGGAGACA GAAGTCCTGG AGATTTGAGT
    ACTGTGAGGA CGAGCGTCCC GCCACCAACG CAGCCGGCTC CAGAACCAGA GCGAAGCCTC
    AGCAGAACCA GCTCAATGCA GCAGCTGGAG CAGTGGGTTC GCACACACCG CACACGAGCT
    CCAGACGACG ACACCAGGAG CATTACGTCC TACCAGACCC TGCCAAGGAA CATGCCGAGC
    CACCGGGCGC AGATTGTCCC TCGCTACCCT GAGGGCTACC GCACTCTGCC CCGAAACATG
    CTGCGTCCGG ACAGCATCTG CAGTGTAGCA GGATCAGTGT ACGACCGAGC GCTTCAGCCC
    AGCACGCAGG AAAAGCGGCG CTCCATCCGA GATGACACCA TGTGGCAGCT GTACGAGTGG
    CAGCAGCGGC AGGCGCACAG TCGGCTTGGG TACGGGACGC TGCCCAGTCC CAAAACTATG
    GGGCAGATCG CGGAGTCCAT CCCCACGTCC CCATCCCATG GCTCTCTGGC CGGATATCAC
    ACCTTCTCCC CGAACAGACC GCTCAACCCT GACTCGCGCT CGGAGCTGTC TTCGCCTGTG
    TTTCGTGGAG ACGTGACCAT CGAGCGACGC CACCGACCAC ACCTGTCCAA GTACTCCTAT
    CCTGCGGAAC GCAGAGCTGC TCCTCCCGCA CAGAACATCA CTGCACAGTC TCTTCAAGGC
    AAAACGCCAG AGGAACTGAC CCTGCTGCTC ATCAAACTGC GGCGGCAGCA GGCCGAGCTC
    AACAGCCTCC GTGAGCACAC ACTCGCGCAG CTCATGCAGC TGAACCTCGA CGCCACCAAC
    CCAAAGAGTG AAATCCTCTC TCATCACCTG CAGAGGAATC TCATGTACTT GGACAGTCAG
    ATGAAGGAGA ACGAACCCAT AATCTTCATG ATTCACACTA TGATTGAGAA CTCGGCGCCC
    CGGCCGCAGC TCTACCAGCA ACAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45113
    Clone ID Related Accession (Same CDS sequence) XM_009300004.2
    Accession Version XM_009300004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
    GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC AACCAGCGAC CAAAGCACCA
    GAGGCGGAGA AGAAGGAGCG TCCCACCAGC ACCATGAGCG AAGCCTCAAA CTACACCGGC
    GGCTCCGACT ACACCACACA TCCCAGCAGC CCCACAACCA GACCATCCCG GTCCTCAAAG
    AAAGTGCACA ACTTTGGGAA AAGGTCCAAC TCCATCAAGA GGAACCCCAA TGCACCTGTG
    ATCAAAAACG GCTGGCTTCA CAAACAGGAC AGCACTGGGA TGAAGATGTG GAAGAAGAGA
    TGGTTCGTTC TGTCTGATAT GTGCCTCTTC TACTACAGAG ATGAGAAGGA AGAGGGGATT
    CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC ATCTCCATGC TGTCCGTGGA CGACCATATT
    ACCCGGAAAT ACGCCTTCAA GGCGACGCAT CCAAACATGC GGACGTATTA TTTCAGCACA
    GACACAGCCA AAGATATGGA GTCCTGGATG AAAGTGATGT CGGATGCGGC GATGGCTCAC
    AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA AAGCTGGACT CTCGCGTTCC TCAGGAGATG
    AACAACATGC TGAACCACCG CGTCTTGACA CGACCCGAGA TCCAAAACAA CGAGAGGAAC
    CGCGAGACGC TCCGCCAAGA GGACAAGAAG AGCAAACCAT CAGAGAAACC TCAGAAAGAC
    AAAGACAGAG TCAGCATGCA GAAAGACGGA GAGCGCTTCA GTGAAAACTA CACTCACCGG
    CAGAAAGACG GCGAGCGCTA CATGCTGCAG AAAGACCTGG AGAAATTTGT CCTGCAGAAA
    GACGGAGAGA AGTACGCCGT GCAGAAAGAA GGCGACAAAT ATCTCCTGCA GAAAGACGGA
    CACAAATACA CAGTGCACAA AGACGGAGAC AAATACACGC TTCAGAGAGA ACTCGAGATG
    TACAATCCAC CAAAAGACGC GGAGAAGATC CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG
    CAGAAGGAGC TGGAGAAACC CGTCCCGCCA CCGCGATCTG AGGAAAAATA CGGCTTTCAG
    AAAGAGGGCA CCATTGAGCG TCCGCTAACT AAAATAAATA GCATTAAACT GCAGCCGGCT
    CAAGCAGCCG CCATCGCTGC AGCCGTTTCA TCCTCTAGGA TGATGCAGAA ACAGGTTAAT
    GGCTCCGGGG AACGAGCGGG AGACAGAAGT CCTGGAGATT TGAGTACTGT GAGGACGAGC
    GTCCCGCCAC CAACGCAGCC GGCTCCAGAA CCAGAGCGAA GCCTCAGCAG AACCAGCTCA
    ATGCAGCAGC TGGAGCAGTG GGTTCGCACA CACCGCACAC GAGCTCCAGA CGACGACACC
    AGGAGCATTA CGTCCTACCA GACCCTGCCA AGGAACATGC CGAGCCACCG GGCGCAGATT
    GTCCCTCGCT ACCCTGAGGG CTACCGCACT CTGCCCCGAA ACATGCTGCG TCCGGACAGC
    ATCTGCAGTG TAGCAGGATC AGTGTACGAC CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG
    CGGCGCTCCA TCCGAGATGA CACCATGTGG CAGCTGTACG AGTGGCAGCA GCGGCAGGCG
    CACAGTCGGC TTGGGTACGG GACGCTGCCC AGTCCCAAAA CTATGGGGCA GATCGCGGAG
    TCCATCCCCA CGTCCCCATC CCATGGCTCT CTGGCCGGAT ATCACACCTT CTCCCCGAAC
    AGACCGCTCA ACCCTGACTC GCGCTCGGAG CTGTCTTCGC CTGTGTTTCG TGGAGACGTG
    ACCATCGAGC GACGCCACCG ACCACACCTG TCCAAGTACT CCTATCCTGC GGAACGCAGA
    GCTGCTCCTC CCGCACAGAA CATCACTGCA CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA
    CTGACCCTGC TGCTCATCAA ACTGCGGCGG CAGCAGGCCG AGCTCAACAG CCTCCGTGAG
    CACACACTCG CGCAGCTCAT GCAGCTGAAC CTCGACGCCA CCAACCCAAA GAGTGAAATC
    CTCTCTCATC ACCTGCAGAG GAATCTCATG TACTTGGACA GTCAGATGAA GGAGAACGAA
    CCCATAATCT TCATGATTCA CACTATGATT GAGAACTCGG CGCCCCGGCC GCAGCTCTAC
    CAGCAACAGA TGAGTCCTGA AGAGTACAGA GAAAACACTT ACATGTACAG GCCGGAGGAG
    CTCGATGTCG ACGCGAAGTT AAGTCGGCTC TGTGAGCAGG ACAAAGTAGT GCGAACTCAA
    GAAGAGAAAC TGCAGCAGCT CCACAGAGAA AAGCACACAC TGGAGACGGC GCTGCTCTCC
    GCCAGTCAGG AGATCGAGAT GAGCTCTGAA AACCCAGCGG CGGTCCAGAG CGTGGTCCAG
    CAGAGGGACG TCCTGCAGAG TGGCCTGCTG AGCACCTGCA GAGAGCTGAG CCGCGTCAGC
    ACTGAGCTGG AGCGAAGCTG GAGAGAATAT GATCGACTAG AGGCCGATGT TACACTTGCC
    AAAACTAACC TGCTGGAGCA GCTGGAGGCC CTCGGCAGCC CACAGACGGA GCCGCCCAGT
    CAAAAGCATG TTCAGATCCA GAAAGAGTTG TGGAGAATTC AGGATGTGAT GGAGGCGCTG
    AGCAAGAACA AACCCAAGAG AAACCCTGAA CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA
    ACCCTGCATA AAAGTGAAGA CTCTGTAGAC TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG
    CCGTACTCAT ATGAAGGAGG CGATCGACCA CCGCCCCTCC CCAACCCCGG TCAACCGCAC
    ACCACCCGCA CAACACCACA CCGACCAGAG GACCGCAAAA CTGCCCAACG CAACGGCACG
    CACACCGGCC CGGATTACAG GCTGTATAAG AGTGAGCCGG AGCTGACCAC AGTAGCCGAA
    GTCGACGAGA GTAACGGTGA AGACAAATCA GAGCAGACAG CAGAGAAAGA GCCGCCCGGC
    GTCACTAAAG GTATAGTTTA CCCGGTGGGA GTGGTCAGTC CCAGAACTAA ATCACCCATG
    CCCGAGTCCT CCACCATCGC CTCCTACGTC ACCTTGAGGA AGACCAAAAA ACCTGATCCC
    AGAACATGTC AGCCGCAGGA CCGTCCGCGC AGCGCTGTGG AGCAGATGAG CAGTGGTGCT
    GAAGTGGGCC GAACACGCAT GAGTGTGGAG GAGCAGATGG AGAGAATCAG ACGACACCAG
    CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA GATGGCTCTC TATCCCGCAG CCTGTCCTTC
    ACTAAGGACA GCACATACTA CACACTGCAG GCACGTAAGA AGAGTCCGGT GATCTCTCCA
    GACGAGCAGG ACGAGCGCAG AGACATGACG CCTGTGGAGC GAGACACAAA ACACACAAAA
    CACCCTGACG CAGAGGAAAA CTGCAGAGAC ACTAAGGAGC AGCTGCCAGC ACACACCCAC
    CTGGAGATTA AGGACAAAGC ATCTGTGACT CGCTCGGTCT CCATGAAAGA GTCTCTCCTG
    AAGAGTGTGA GTCCCAAACC CGTGCTGAAG CAGGACGAAT CTGAGTGTAA ACTGGAGTAC
    GACAGAAAGC TGGCCACTCA GAACAGCCTG CAGACGGCAG TGATGGTGCG TGTGGGCACT
    GAGGAGGATG AAGAGGATGA AGAAGAGGAG CAGAAAGACT CAGTTCAGCA GCAGGAGGAC
    TCTTATGAGC TCAGCAACAG CAAACACAGC ACACTGATCT CAGCATCATG TGACAGGAAG
    AACACATCAT CACCATCGTC ATCATCAGAC TGGAAGTCTC GCCCCCTCCT TCATCAGCTG
    CCCAACACAA ACACAACAGC GTCCAGGCCT CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298279.1
    CDS123..4070
    Translation

    Target ORF information:

    RefSeq Version XM_009300004.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGCAGA TGTGCTGCCA CAATGAGCGA AAGGTGACCT GCAAGCATCC CGTCAGCGGC 
    GTCCCGTCAC AAGACAACTG CATCTTTGTG GTCAACGAAC AACCAGCGAC CAAAGCACCA
    GAGGCGGAGA AGAAGGAGCG TCCCACCAGC ACCATGAGCG AAGCCTCAAA CTACACCGGC
    GGCTCCGACT ACACCACACA TCCCAGCAGC CCCACAACCA GACCATCCCG GTCCTCAAAG
    AAAGTGCACA ACTTTGGGAA AAGGTCCAAC TCCATCAAGA GGAACCCCAA TGCACCTGTG
    ATCAAAAACG GCTGGCTTCA CAAACAGGAC AGCACTGGGA TGAAGATGTG GAAGAAGAGA
    TGGTTCGTTC TGTCTGATAT GTGCCTCTTC TACTACAGAG ATGAGAAGGA AGAGGGGATT
    CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC ATCTCCATGC TGTCCGTGGA CGACCATATT
    ACCCGGAAAT ACGCCTTCAA GGCGACGCAT CCAAACATGC GGACGTATTA TTTCAGCACA
    GACACAGCCA AAGATATGGA GTCCTGGATG AAAGTGATGT CGGATGCGGC GATGGCTCAC
    AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA AAGCTGGACT CTCGCGTTCC TCAGGAGATG
    AACAACATGC TGAACCACCG CGTCTTGACA CGACCCGAGA TCCAAAACAA CGAGAGGAAC
    CGCGAGACGC TCCGCCAAGA GGACAAGAAG AGCAAACCAT CAGAGAAACC TCAGAAAGAC
    AAAGACAGAG TCAGCATGCA GAAAGACGGA GAGCGCTTCA GTGAAAACTA CACTCACCGG
    CAGAAAGACG GCGAGCGCTA CATGCTGCAG AAAGACCTGG AGAAATTTGT CCTGCAGAAA
    GACGGAGAGA AGTACGCCGT GCAGAAAGAA GGCGACAAAT ATCTCCTGCA GAAAGACGGA
    CACAAATACA CAGTGCACAA AGACGGAGAC AAATACACGC TTCAGAGAGA ACTCGAGATG
    TACAATCCAC CAAAAGACGC GGAGAAGATC CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG
    CAGAAGGAGC TGGAGAAACC CGTCCCGCCA CCGCGATCTG AGGAAAAATA CGGCTTTCAG
    AAAGAGGGCA CCATTGAGCG TCCGCTAACT AAAATAAATA GCATTAAACT GCAGCCGGCT
    CAAGCAGCCG CCATCGCTGC AGCCGTTTCA TCCTCTAGGA TGATGCAGAA ACAGGTTAAT
    GGCTCCGGGG AACGAGCGGG AGACAGAAGT CCTGGAGATT TGAGTACTGT GAGGACGAGC
    GTCCCGCCAC CAACGCAGCC GGCTCCAGAA CCAGAGCGAA GCCTCAGCAG AACCAGCTCA
    ATGCAGCAGC TGGAGCAGTG GGTTCGCACA CACCGCACAC GAGCTCCAGA CGACGACACC
    AGGAGCATTA CGTCCTACCA GACCCTGCCA AGGAACATGC CGAGCCACCG GGCGCAGATT
    GTCCCTCGCT ACCCTGAGGG CTACCGCACT CTGCCCCGAA ACATGCTGCG TCCGGACAGC
    ATCTGCAGTG TAGCAGGATC AGTGTACGAC CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG
    CGGCGCTCCA TCCGAGATGA CACCATGTGG CAGCTGTACG AGTGGCAGCA GCGGCAGGCG
    CACAGTCGGC TTGGGTACGG GACGCTGCCC AGTCCCAAAA CTATGGGGCA GATCGCGGAG
    TCCATCCCCA CGTCCCCATC CCATGGCTCT CTGGCCGGAT ATCACACCTT CTCCCCGAAC
    AGACCGCTCA ACCCTGACTC GCGCTCGGAG CTGTCTTCGC CTGTGTTTCG TGGAGACGTG
    ACCATCGAGC GACGCCACCG ACCACACCTG TCCAAGTACT CCTATCCTGC GGAACGCAGA
    GCTGCTCCTC CCGCACAGAA CATCACTGCA CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA
    CTGACCCTGC TGCTCATCAA ACTGCGGCGG CAGCAGGCCG AGCTCAACAG CCTCCGTGAG
    CACACACTCG CGCAGCTCAT GCAGCTGAAC CTCGACGCCA CCAACCCAAA GAGTGAAATC
    CTCTCTCATC ACCTGCAGAG GAATCTCATG TACTTGGACA GTCAGATGAA GGAGAACGAA
    CCCATAATCT TCATGATTCA CACTATGATT GAGAACTCGG CGCCCCGGCC GCAGCTCTAC
    CAGCAACAGA TGAGTCCTGA AGAGTACAGA GAAAACACTT ACATGTACAG GCCGGAGGAG
    CTCGATGTCG ACGCGAAGTT AAGTCGGCTC TGTGAGCAGG ACAAAGTAGT GCGAACTCAA
    GAAGAGAAAC TGCAGCAGCT CCACAGAGAA AAGCACACAC TGGAGACGGC GCTGCTCTCC
    GCCAGTCAGG AGATCGAGAT GAGCTCTGAA AACCCAGCGG CGGTCCAGAG CGTGGTCCAG
    CAGAGGGACG TCCTGCAGAG TGGCCTGCTG AGCACCTGCA GAGAGCTGAG CCGCGTCAGC
    ACTGAGCTGG AGCGAAGCTG GAGAGAATAT GATCGACTAG AGGCCGATGT TACACTTGCC
    AAAACTAACC TGCTGGAGCA GCTGGAGGCC CTCGGCAGCC CACAGACGGA GCCGCCCAGT
    CAAAAGCATG TTCAGATCCA GAAAGAGTTG TGGAGAATTC AGGATGTGAT GGAGGCGCTG
    AGCAAGAACA AACCCAAGAG AAACCCTGAA CCCAGTTTTC CTGGTGCAAA CCCTCTGTCA
    ACCCTGCATA AAAGTGAAGA CTCTGTAGAC TCCTCGTGTG TTCCTCCTCG TCCTCCTCTG
    CCGTACTCAT ATGAAGGAGG CGATCGACCA CCGCCCCTCC CCAACCCCGG TCAACCGCAC
    ACCACCCGCA CAACACCACA CCGACCAGAG GACCGCAAAA CTGCCCAACG CAACGGCACG
    CACACCGGCC CGGATTACAG GCTGTATAAG AGTGAGCCGG AGCTGACCAC AGTAGCCGAA
    GTCGACGAGA GTAACGGTGA AGACAAATCA GAGCAGACAG CAGAGAAAGA GCCGCCCGGC
    GTCACTAAAG GTATAGTTTA CCCGGTGGGA GTGGTCAGTC CCAGAACTAA ATCACCCATG
    CCCGAGTCCT CCACCATCGC CTCCTACGTC ACCTTGAGGA AGACCAAAAA ACCTGATCCC
    AGAACATGTC AGCCGCAGGA CCGTCCGCGC AGCGCTGTGG AGCAGATGAG CAGTGGTGCT
    GAAGTGGGCC GAACACGCAT GAGTGTGGAG GAGCAGATGG AGAGAATCAG ACGACACCAG
    CAGGGGGCGC TGAGAGAGCG CAGGAGAGAA GATGGCTCTC TATCCCGCAG CCTGTCCTTC
    ACTAAGGACA GCACATACTA CACACTGCAG GCACGTAAGA AGAGTCCGGT GATCTCTCCA
    GACGAGCAGG ACGAGCGCAG AGACATGACG CCTGTGGAGC GAGACACAAA ACACACAAAA
    CACCCTGACG CAGAGGAAAA CTGCAGAGAC ACTAAGGAGC AGCTGCCAGC ACACACCCAC
    CTGGAGATTA AGGACAAAGC ATCTGTGACT CGCTCGGTCT CCATGAAAGA GTCTCTCCTG
    AAGAGTGTGA GTCCCAAACC CGTGCTGAAG CAGGACGAAT CTGAGTGTAA ACTGGAGTAC
    GACAGAAAGC TGGCCACTCA GAACAGCCTG CAGACGGCAG TGATGGTGCG TGTGGGCACT
    GAGGAGGATG AAGAGGATGA AGAAGAGGAG CAGAAAGACT CAGTTCAGCA GCAGGAGGAC
    TCTTATGAGC TCAGCAACAG CAAACACAGC ACACTGATCT CAGCATCATG TGACAGGAAG
    AACACATCAT CACCATCGTC ATCATCAGAC TGGAAGTCTC GCCCCCTCCT TCATCAGCTG
    CCCAACACAA ACACAACAGC GTCCAGGCCT CAGTTCAACA GCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45114
    Clone ID Related Accession (Same CDS sequence) XM_009300005.2
    Accession Version XM_009300005.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GCATCATGTG ACAGGAAGAA CACATCATCA CCATCGTCAT CATCAGACTG GAAGTCTCGC
    CCCCTCCTTC ATCAGCTGCC CAACACAAAC ACAACAGCGT CCAGGCCTCA GTTCAACAGC
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298280.1
    CDS43..3948
    Translation

    Target ORF information:

    RefSeq Version XM_009300005.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GCATCATGTG ACAGGAAGAA CACATCATCA CCATCGTCAT CATCAGACTG GAAGTCTCGC
    CCCCTCCTTC ATCAGCTGCC CAACACAAAC ACAACAGCGT CCAGGCCTCA GTTCAACAGC
    GACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45115
    Clone ID Related Accession (Same CDS sequence) XM_009300006.2
    Accession Version XM_009300006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298281.1
    CDS43..3945
    Translation

    Target ORF information:

    RefSeq Version XM_009300006.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCAGATGAGT CCTGAAGAGT ACAGAGAAAA CACTTACATG
    TACAGGCCGG AGGAGCTCGA TGTCGACGCG AAGTTAAGTC GGCTCTGTGA GCAGGACAAA
    GTAGTGCGAA CTCAAGAAGA GAAACTGCAG CAGCTCCACA GAGAAAAGCA CACACTGGAG
    ACGGCGCTGC TCTCCGCCAG TCAGGAGATC GAGATGAGCT CTGAAAACCC AGCGGCGGTC
    CAGAGCGTGG TCCAGCAGAG GGACGTCCTG CAGAGTGGCC TGCTGAGCAC CTGCAGAGAG
    CTGAGCCGCG TCAGCACTGA GCTGGAGCGA AGCTGGAGAG AATATGATCG ACTAGAGGCC
    GATGTTACAC TTGCCAAAAC TAACCTGCTG GAGCAGCTGG AGGCCCTCGG CAGCCCACAG
    ACGGAGCCGC CCAGTCAAAA GCATGTTCAG ATCCAGAAAG AGTTGTGGAG AATTCAGGAT
    GTGATGGAGG CGCTGAGCAA GAACAAACCC AAGAGAAACC CTGAACCCAG TTTTCCTGGT
    GCAAACCCTC TGTCAACCCT GCATAAAAGT GAAGACTCTG TAGACTCCTC GTGTGTTCCT
    CCTCGTCCTC CTCTGCCGTA CTCATATGAA GGAGGCGATC GACCACCGCC CCTCCCCAAC
    CCCGGTCAAC CGCACACCAC CCGCACAACA CCACACCGAC CAGAGGACCG CAAAACTGCC
    CAACGCAACG GCACGCACAC CGGCCCGGAT TACAGGCTGT ATAAGAGTGA GCCGGAGCTG
    ACCACAGTAG CCGAAGTCGA CGAGAGTAAC GGTGAAGACA AATCAGAGCA GACAGCAGAG
    AAAGAGCCGC CCGGCGTCAC TAAAGGTATA GTTTACCCGG TGGGAGTGGT CAGTCCCAGA
    ACTAAATCAC CCATGCCCGA GTCCTCCACC ATCGCCTCCT ACGTCACCTT GAGGAAGACC
    AAAAAACCTG ATCCCAGAAC ATGTCAGCCG CAGGACCGTC CGCGCAGCGC TGTGGAGCAG
    ATGAGCAGTG GTGCTGAAGT GGGCCGAACA CGCATGAGTG TGGAGGAGCA GATGGAGAGA
    ATCAGACGAC ACCAGCAGGG GGCGCTGAGA GAGCGCAGGA GAGAAGATGG CTCTCTATCC
    CGCAGCCTGT CCTTCACTAA GGACAGCACA TACTACACAC TGCAGGCACG TAAGAAGAGT
    CCGGTGATCT CTCCAGACGA GCAGGACGAG CGCAGAGACA TGACGCCTGT GGAGCGAGAC
    ACAAAACACA CAAAACACCC TGACGCAGAG GAAAACTGCA GAGACACTAA GGAGCAGCTG
    CCAGCACACA CCCACCTGGA GATTAAGGAC AAAGCATCTG TGACTCGCTC GGTCTCCATG
    AAAGAGTCTC TCCTGAAGAG TGTGAGTCCC AAACCCGTGC TGAAGCAGGA CGAATCTGAG
    TGTAAACTGG AGTACGACAG AAAGCTGGCC ACTCAGAACA GCCTGCAGAC GGCAGTGATG
    GTGCGTGTGG GCACTGAGGA GGATGAAGAG GATGAAGAAG AGGAGCAGAA AGACTCAGTT
    CAGCAGCAGG AGGACTCTTA TGAGCTCAGC AACAGCAAAC ACAGCACACT GATCTCAGCA
    TCATGTGACA GGAAGAACAC ATCATCACCA TCGTCATCAT CAGACTGGAA GTCTCGCCCC
    CTCCTTCATC AGCTGCCCAA CACAAACACA ACAGCGTCCA GGCCTCAGTT CAACAGCGAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45116
    Clone ID Related Accession (Same CDS sequence) XM_009300008.2
    Accession Version XM_009300008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GACTCTGTAG ACTCCTCGTG TGTTCCTCCT
    CGTCCTCCTC TGCCGTACTC ATATGAAGGA GGCGATCGAC CACCGCCCCT CCCCAACCCC
    GGTCAACCGC ACACCACCCG CACAACACCA CACCGACCAG AGGACCGCAA AACTGCCCAA
    CGCAACGGCA CGCACACCGG CCCGGATTAC AGGCTGTATA AGAGTGAGCC GGAGCTGACC
    ACAGTAGCCG AAGTCGACGA GAGTAACGGT GAAGACAAAT CAGAGCAGAC AGCAGAGAAA
    GAGCCGCCCG GCGTCACTAA AGGTATAGTT TACCCGGTGG GAGTGGTCAG TCCCAGAACT
    AAATCACCCA TGCCCGAGTC CTCCACCATC GCCTCCTACG TCACCTTGAG GAAGACCAAA
    AAACCTGATC CCAGAACATG TCAGCCGCAG GACCGTCCGC GCAGCGCTGT GGAGCAGATG
    AGCAGTGGTG CTGAAGTGGG CCGAACACGC ATGAGTGTGG AGGAGCAGAT GGAGAGAATC
    AGACGACACC AGCAGGGGGC GCTGAGAGAG CGCAGGAGAG AAGATGGCTC TCTATCCCGC
    AGCCTGTCCT TCACTAAGGA CAGCACATAC TACACACTGC AGGCACGTAA GAAGAGTCCG
    GTGATCTCTC CAGACGAGCA GGACGAGCGC AGAGACATGA CGCCTGTGGA GCGAGACACA
    AAACACACAA AACACCCTGA CGCAGAGGAA AACTGCAGAG ACACTAAGGA GCAGCTGCCA
    GCACACACCC ACCTGGAGAT TAAGGACAAA GCATCTGTGA CTCGCTCGGT CTCCATGAAA
    GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA CCCGTGCTGA AGCAGGACGA ATCTGAGTGT
    AAACTGGAGT ACGACAGAAA GCTGGCCACT CAGAACAGCC TGCAGACGGC AGTGATGGTG
    CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG
    CAGCAGGAGG ACTCTTATGA GCTCAGCAAC AGCAAACACA GCACACTGAT CTCAGCATCA
    TGTGACAGGA AGAACACATC ATCACCATCG TCATCATCAG ACTGGAAGTC TCGCCCCCTC
    CTTCATCAGC TGCCCAACAC AAACACAACA GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298283.1
    CDS43..3942
    Translation

    Target ORF information:

    RefSeq Version XM_009300008.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GACTCTGTAG ACTCCTCGTG TGTTCCTCCT
    CGTCCTCCTC TGCCGTACTC ATATGAAGGA GGCGATCGAC CACCGCCCCT CCCCAACCCC
    GGTCAACCGC ACACCACCCG CACAACACCA CACCGACCAG AGGACCGCAA AACTGCCCAA
    CGCAACGGCA CGCACACCGG CCCGGATTAC AGGCTGTATA AGAGTGAGCC GGAGCTGACC
    ACAGTAGCCG AAGTCGACGA GAGTAACGGT GAAGACAAAT CAGAGCAGAC AGCAGAGAAA
    GAGCCGCCCG GCGTCACTAA AGGTATAGTT TACCCGGTGG GAGTGGTCAG TCCCAGAACT
    AAATCACCCA TGCCCGAGTC CTCCACCATC GCCTCCTACG TCACCTTGAG GAAGACCAAA
    AAACCTGATC CCAGAACATG TCAGCCGCAG GACCGTCCGC GCAGCGCTGT GGAGCAGATG
    AGCAGTGGTG CTGAAGTGGG CCGAACACGC ATGAGTGTGG AGGAGCAGAT GGAGAGAATC
    AGACGACACC AGCAGGGGGC GCTGAGAGAG CGCAGGAGAG AAGATGGCTC TCTATCCCGC
    AGCCTGTCCT TCACTAAGGA CAGCACATAC TACACACTGC AGGCACGTAA GAAGAGTCCG
    GTGATCTCTC CAGACGAGCA GGACGAGCGC AGAGACATGA CGCCTGTGGA GCGAGACACA
    AAACACACAA AACACCCTGA CGCAGAGGAA AACTGCAGAG ACACTAAGGA GCAGCTGCCA
    GCACACACCC ACCTGGAGAT TAAGGACAAA GCATCTGTGA CTCGCTCGGT CTCCATGAAA
    GAGTCTCTCC TGAAGAGTGT GAGTCCCAAA CCCGTGCTGA AGCAGGACGA ATCTGAGTGT
    AAACTGGAGT ACGACAGAAA GCTGGCCACT CAGAACAGCC TGCAGACGGC AGTGATGGTG
    CGTGTGGGCA CTGAGGAGGA TGAAGAGGAT GAAGAAGAGG AGCAGAAAGA CTCAGTTCAG
    CAGCAGGAGG ACTCTTATGA GCTCAGCAAC AGCAAACACA GCACACTGAT CTCAGCATCA
    TGTGACAGGA AGAACACATC ATCACCATCG TCATCATCAG ACTGGAAGTC TCGCCCCCTC
    CTTCATCAGC TGCCCAACAC AAACACAACA GCGTCCAGGC CTCAGTTCAA CAGCGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45117
    Clone ID Related Accession (Same CDS sequence) XM_009300009.2
    Accession Version XM_009300009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGCAT CATGTGACAG GAAGAACACA TCATCACCAT CGTCATCATC AGACTGGAAG
    TCTCGCCCCC TCCTTCATCA GCTGCCCAAC ACAAACACAA CAGCGTCCAG GCCTCAGTTC
    AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298284.1
    CDS43..3774
    Translation

    Target ORF information:

    RefSeq Version XM_009300009.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGCAT CATGTGACAG GAAGAACACA TCATCACCAT CGTCATCATC AGACTGGAAG
    TCTCGCCCCC TCCTTCATCA GCTGCCCAAC ACAAACACAA CAGCGTCCAG GCCTCAGTTC
    AACAGCGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45118
    Clone ID Related Accession (Same CDS sequence) XM_009300010.2 , XM_009300010.3
    Accession Version XM_009300010.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA
    CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298285.1
    CDS43..3930
    Translation

    Target ORF information:

    RefSeq Version XM_009300010.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA
    CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45118
    Clone ID Related Accession (Same CDS sequence) XM_009300010.2 , XM_009300010.3
    Accession Version XM_009300010.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA
    CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298285.1
    CDS42..3929
    Translation

    Target ORF information:

    RefSeq Version XM_009300010.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300010.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAG TCCTGAAGAG TACAGAGAAA ACACTTACAT GTACAGGCCG
    GAGGAGCTCG ATGTCGACGC GAAGTTAAGT CGGCTCTGTG AGCAGGACAA AGTAGTGCGA
    ACTCAAGAAG AGAAACTGCA GCAGCTCCAC AGAGAAAAGC ACACACTGGA GACGGCGCTG
    CTCTCCGCCA GTCAGGAGAT CGAGATGAGC TCTGAAAACC CAGCGGCGGT CCAGAGCGTG
    GTCCAGCAGA GGGACGTCCT GCAGAGTGGC CTGCTGAGCA CCTGCAGAGA GCTGAGCCGC
    GTCAGCACTG AGCTGGAGCG AAGCTGGAGA GAATATGATC GACTAGAGGC CGATGTTACA
    CTTGCCAAAA CTAACCTGCT GGAGCAGCTG GAGGCCCTCG GCAGCCCACA GACGGAGCCG
    CCCAGTCAAA AGCATGTTCA GATCCAGAAA GAGTTGTGGA GAATTCAGGA TGTGATGGAG
    GCGCTGAGCA AGAACAAACC CAAGAGAAAC CCTGAACCCA GTTTTCCTGG TGCAAACCCT
    CTGTCAACCC TGCATAAAAG TGAAGGCCCG GATTACAGGC TGTATAAGAG TGAGCCGGAG
    CTGACCACAG TAGCCGAAGT CGACGAGAGT AACGGTGAAG ACAAATCAGA GCAGACAGCA
    GAGAAAGAGC CGCCCGGCGT CACTAAAGGT ATAGTTTACC CGGTGGGAGT GGTCAGTCCC
    AGAACTAAAT CACCCATGCC CGAGTCCTCC ACCATCGCCT CCTACGTCAC CTTGAGGAAG
    ACCAAAAAAC CTGATCCCAG AACATGTCAG CCGCAGGACC GTCCGCGCAG CGCTGTGGAG
    CAGATGAGCA GTGGTGCTGA AGTGGGCCGA ACACGCATGA GTGTGGAGGA GCAGATGGAG
    AGAATCAGAC GACACCAGCA GGGGGCGCTG AGAGAGCGCA GGAGAGAAGA TGGCTCTCTA
    TCCCGCAGCC TGTCCTTCAC TAAGGACAGC ACATACTACA CACTGCAGGC ACGTAAGAAG
    AGTCCGGTGA TCTCTCCAGA CGAGCAGGAC GAGCGCAGAG ACATGACGCC TGTGGAGCGA
    GACACAAAAC ACACAAAACA CCCTGACGCA GAGGAAAACT GCAGAGACAC TAAGGAGCAG
    CTGCCAGCAC ACACCCACCT GGAGATTAAG GACAAAGCAT CTGTGACTCG CTCGGTCTCC
    ATGAAAGAGT CTCTCCTGAA GAGTGTGAGT CCCAAACCCG TGCTGAAGCA GGACGAATCT
    GAGTGTAAAC TGGAGTACGA CAGAAAGCTG GCCACTCAGA ACAGCCTGCA GACGGCAGTG
    ATGGTGCGTG TGGGCACTGA GGAGGATGAA GAGGATGAAG AAGAGGAGCA GAAAGACTCA
    GTTCAGCAGC AGGAGGACTC TTATGAGCTC AGCAACAGCA AACACAGCAC ACTGATCTCA
    GTGAACAGTG TGTCGACTCC TCCTCAATCT CCAAGCTCCT CCTCCTCTCC TCCGCCTCCA
    CCCTCCCCTC CACAGCTGAC GGACGGGTCA CACTTCATGT GTGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45119
    Clone ID Related Accession (Same CDS sequence) XM_009300011.2 , XM_009300011.3
    Accession Version XM_009300011.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG
    CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298286.1
    CDS43..3756
    Translation

    Target ORF information:

    RefSeq Version XM_009300011.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG
    CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45119
    Clone ID Related Accession (Same CDS sequence) XM_009300011.2 , XM_009300011.3
    Accession Version XM_009300011.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG
    CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298286.1
    CDS42..3755
    Translation

    Target ORF information:

    RefSeq Version XM_009300011.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300011.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GATGAGTCCT GAAGAGTACA GAGAAAACAC TTACATGTAC
    AGGCCGGAGG AGCTCGATGT CGACGCGAAG TTAAGTCGGC TCTGTGAGCA GGACAAAGTA
    GTGCGAACTC AAGAAGAGAA ACTGCAGCAG CTCCACAGAG AAAAGCACAC ACTGGAGACG
    GCGCTGCTCT CCGCCAGTCA GGAGATCGAG ATGAGCTCTG AAAACCCAGC GGCGGTCCAG
    AGCGTGGTCC AGCAGAGGGA CGTCCTGCAG AGTGGCCTGC TGAGCACCTG CAGAGAGCTG
    AGCCGCGTCA GCACTGAGCT GGAGCGAAGC TGGAGAGAAT ATGATCGACT AGAGGCCGAT
    GTTACACTTG CCAAAACTAA CCTGCTGGAG CAGCTGGAGG CCCTCGGCAG CCCACAGACG
    GAGCCGCCCA GTCAAAAGCA TGTTCAGATC CAGAAAGAGT TGTGGAGAAT TCAGGATGTG
    ATGGAGGCGC TGAGCAAGAA CAAACCCAAG AGAAACCCTG AACCCAGTTT TCCTGGTGCA
    AACCCTCTGT CAACCCTGCA TAAAAGTGAA GGCCCGGATT ACAGGCTGTA TAAGAGTGAG
    CCGGAGCTGA CCACAGTAGC CGAAGTCGAC GAGAGTAACG GTGAAGACAA ATCAGAGCAG
    ACAGCAGAGA AAGAGCCGCC CGGCGTCACT AAAGGTATAG TTTACCCGGT GGGAGTGGTC
    AGTCCCAGAA CTAAATCACC CATGCCCGAG TCCTCCACCA TCGCCTCCTA CGTCACCTTG
    AGGAAGACCA AAAAACCTGA TCCCAGAACA TGTCAGCCGC AGGACCGTCC GCGCAGCGCT
    GTGGAGCAGA TGAGCAGTGG TGCTGAAGTG GGCCGAACAC GCATGAGTGT GGAGGAGCAG
    ATGGAGAGAA TCAGACGACA CCAGCAGGGG GCGCTGAGAG AGCGCAGGAG AGAAGATGGC
    TCTCTATCCC GCAGCCTGTC CTTCACTAAG GACAGCACAT ACTACACACT GCAGGCACGT
    AAGAAGAGTC CGGTGATCTC TCCAGACGAG CAGGACGAGC GCAGAGACAT GACGCCTGTG
    GAGCGAGACA CAAAACACAC AAAACACCCT GACGCAGAGG AAAACTGCAG AGACACTAAG
    GAGCAGCTGC CAGCACACAC CCACCTGGAG ATTAAGGACA AAGCATCTGT GACTCGCTCG
    GTCTCCATGA AAGAGTCTCT CCTGAAGAGT GTGAGTCCCA AACCCGTGCT GAAGCAGGAC
    GAATCTGAGT GTAAACTGGA GTACGACAGA AAGCTGGCCA CTCAGAACAG CCTGCAGACG
    GCAGTGATGG TGCGTGTGGG CACTGAGGAG GATGAAGAGG ATGAAGAAGA GGAGCAGAAA
    GACTCAGTTC AGCAGCAGGA GGACTCTTAT GAGCTCAGCA ACAGCAAACA CAGCACACTG
    ATCTCAGTGA ACAGTGTGTC GACTCCTCCT CAATCTCCAA GCTCCTCCTC CTCTCCTCCG
    CCTCCACCCT CCCCTCCACA GCTGACGGAC GGGTCACACT TCATGTGTGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45120
    Clone ID Related Accession (Same CDS sequence) XM_009300012.2
    Accession Version XM_009300012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pleckstrin homology domain-containing family A member 5 isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT
    CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009298287.1
    CDS44..4180
    Translation

    Target ORF information:

    RefSeq Version XM_009300012.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pleckstrin homology domain containing, family A member 5 (plekha5), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG ACCTGAACCC GGACTGGCTG TCTTGCCTGC CTTCCTCTTG GAGTTACGGC 
    GTGACCCGGG ACGGACGAGT GTTCTTTATC AATGAAGAAG CGAAGAGCAC AACCTGGCTG
    CATCCAGTCA CAGGAGAAGC CGTCATTACA GGACACAGAA AAACTCCAGA TTTACCCACC
    GGATGGGAAG AAGGATACAC GTTTGAAGGA GCCCGTTGCT TTATCAACCA CAATGAGCGA
    AAGGTGACCT GCAAGCATCC CGTCAGCGGC GTCCCGTCAC AAGACAACTG CATCTTTGTG
    GTCAACGAAC AACCAGCGAC CAAAGCACCA GAGGCGGAGA AGAAGGAGCG TCCCACCAGC
    ACCATGAGCG AAGCCTCAAA CTACACCGGC GGCTCCGACT ACACCACACA TCCCAGCAGC
    CCCACAACCA GACCATCCCG GTCCTCAAAG AAAGTGCACA ACTTTGGGAA AAGGTCCAAC
    TCCATCAAGA GGAACCCCAA TGCACCTGTG ATCAAAAACG GCTGGCTTCA CAAACAGGAC
    AGCACTGGGA TGAAGATGTG GAAGAAGAGA TGGTTCGTTC TGTCTGATAT GTGCCTCTTC
    TACTACAGAG ATGAGAAGGA AGAGGGGATT CTGGGAAGCA TCCTCCTGCC TAGCTTTCAC
    ATCTCCATGC TGTCCGTGGA CGACCATATT ACCCGGAAAT ACGCCTTCAA GGCGACGCAT
    CCAAACATGC GGACGTATTA TTTCAGCACA GACACAGCCA AAGATATGGA GTCCTGGATG
    AAAGTGATGT CGGATGCGGC GATGGCTCAC AGCGAGCCAA TCAGAAGGCT TGAGAAGGTA
    AAGCTGGACT CTCGCGTTCC TCAGGAGATG AACAACATGC TGAACCACCG CGTCTTGACA
    CGACCCGAGA TCCAAAACAA CGAGAGGAAC CGCGAGACGC TCCGCCAAGA GGACAAGAAG
    AGCAAACCAT CAGAGAAACC TCAGAAAGAC AAAGACAGAG TCAGCATGCA GAAAGACGGA
    GAGCGCTTCA GTGAAAACTA CACTCACCGG CAGAAAGACG GCGAGCGCTA CATGCTGCAG
    AAAGACCTGG AGAAATTTGT CCTGCAGAAA GACGGAGAGA AGTACGCCGT GCAGAAAGAA
    GGCGACAAAT ATCTCCTGCA GAAAGACGGA CACAAATACA CAGTGCACAA AGACGGAGAC
    AAATACACGC TTCAGAGAGA ACTCGAGATG TACAATCCAC CAAAAGACGC GGAGAAGATC
    CCGCAGAAAG ATGCAAGGTT TAGTCTGGTG CAGAAGGAGC TGGAGAAACC CGTCCCGCCA
    CCGCGATCTG AGGAAAAATA CGGCTTTCAG AAAGAGGGCA CCATTGAGCG TCCGCTAACT
    AAAATAAATA GCATTAAACT GCAGCCGGCT CAAGCAGCCG CCATCGCTGC AGCCGTTTCA
    TCCTCTAGGA TGATGCAGAA ACAGGTTAAT GGCTCCGGGG AACGAGCGGG AGACAGAAGT
    CCTGGAGATT TGAGTACTGT GAGGACGAGC GTCCCGCCAC CAACGCAGCC GGCTCCAGAA
    CCAGAGCGAA GCCTCAGCAG AACCAGCTCA ATGCAGCAGC TGGAGCAGTG GGTTCGCACA
    CACCGCACAC GAGCTCCAGA CGACGACACC AGGAGCATTA CGTCCTACCA GACCCTGCCA
    AGGAACATGC CGAGCCACCG GGCGCAGATT GTCCCTCGCT ACCCTGAGGG CTACCGCACT
    CTGCCCCGAA ACATGCTGCG TCCGGACAGC ATCTGCAGTG TAGCAGGATC AGTGTACGAC
    CGAGCGCTTC AGCCCAGCAC GCAGGAAAAG CGGCGCTCCA TCCGAGATGA CACCATGTGG
    CAGCTGTACG AGTGGCAGCA GCGGCAGGCG CACAGTCGGC TTGGGTACGG GACGCTGCCC
    AGTCCCAAAA CTATGGGGCA GATCGCGGAG TCCATCCCCA CGTCCCCATC CCATGGCTCT
    CTGGCCGGAT ATCACACCTT CTCCCCGAAC AGACCGCTCA ACCCTGACTC GCGCTCGGAG
    CTGTCTTCGC CTGTGTTTCG TGGAGACGTG ACCATCGAGC GACGCCACCG ACCACACCTG
    TCCAAGTACT CCTATCCTGC GGAACGCAGA GCTGCTCCTC CCGCACAGAA CATCACTGCA
    CAGTCTCTTC AAGGCAAAAC GCCAGAGGAA CTGACCCTGC TGCTCATCAA ACTGCGGCGG
    CAGCAGGCCG AGCTCAACAG CCTCCGTGAG CACACACTCG CGCAGCTCAT GCAGCTGAAC
    CTCGACGCCA CCAACCCAAA GAGTGAAATC CTCTCTCATC ACCTGCAGAG GAATCTCATG
    TACTTGGACA GTCAGATGAA GGAGAACGAA CCCATAATCT TCATGATTCA CACTATGATT
    GAGAACTCGG CGCCCCGGCC GCAGCTCTAC CAGCAAATGA GTCCTGAAGA GTACAGAGAA
    AACACTTACA TGTACAGGCC GGAGGAGCTC GATGTCGACG CGAAGTTAAG TCGGCTCTGT
    GAGCAGGACA AAGTAGTGCG AACTCAAGAA GAGAAACTGC AGCAGCTCCA CAGAGAAAAG
    CACACACTGG AGACGGCGCT GCTCTCCGCC AGTCAGGAGA TCGAGATGAG CTCTGAAAAC
    CCAGCGGCGG TCCAGAGCGT GGTCCAGCAG AGGGACGTCC TGCAGAGTGG CCTGCTGAGC
    ACCTGCAGAG AGCTGAGCCG CGTCAGCACT GAGCTGGAGC GAAGCTGGAG AGAATATGAT
    CGACTAGAGG CCGATGTTAC ACTTGCCAAA ACTAACCTGC TGGAGCAGCT GGAGGCCCTC
    GGCAGCCCAC AGACGGAGCC GCCCAGTCAA AAGCATGTTC AGATCCAGAA AGAGTTGTGG
    AGAATTCAGG ATGTGATGGA GGCGCTGAGC AAGAACAAAC CCAAGAGAAA CCCTGAACCC
    AGTTTTCCTG GTGCAAACCC TCTGTCAACC CTGCATAAAA GTGAAGACTC TGTAGACTCC
    TCGTGTGTTC CTCCTCGTCC TCCTCTGCCG TACTCATATG AAGGAGGCGA TCGACCACCG
    CCCCTCCCCA ACCCCGGTCA ACCGCACACC ACCCGCACAA CACCACACCG ACCAGAGGAC
    CGCAAAACTG CCCAACGCAA CGGCACGCAC ACCGGCCCGG ATTACAGGCT GTATAAGAGT
    GAGCCGGAGC TGACCACAGT AGCCGAAGTC GACGAGAGTA ACGGTGAAGA CAAATCAGAG
    CAGACAGCAG AGAAAGAGCC GCCCGGCGTC ACTAAAGGTA TAGTTTACCC GGTGGGAGTG
    GTCAGTCCCA GAACTAAATC ACCCATGCCC GAGTCCTCCA CCATCGCCTC CTACGTCACC
    TTGAGGAAGA CCAAAAAACC TGATCCCAGA ACATGTCAGC CGCAGGACCG TCCGCGCAGC
    GCTGTGGAGC AGATGAGCAG TGGTGCTGAA GTGGGCCGAA CACGCATGAG TGTGGAGGAG
    CAGATGGAGA GAATCAGACG ACACCAGCAG GGGGCGCTGA GAGAGCGCAG GAGAGAAGAT
    GGCTCTCTAT CCCGCAGCCT GTCCTTCACT AAGGACAGCA CATACTACAC ACTGCAGGCA
    CGTAAGAAGA GTCCGGTGAT CTCTCCAGAC GAGCAGGACG AGCGCAGAGA CATGACGCCT
    GTGGAGCGAG ACACAAAACA CACAAAACAC CCTGACGCAG AGGAAAACTG CAGAGACACT
    AAGGAGCAGC TGCCAGCACA CACCCACCTG GAGATTAAGG ACAAAGCATC TGTGACTCGC
    TCGGTCTCCA TGAAAGAGTC TCTCCTGAAG AGTGTGAGTC CCAAACCCGT GCTGAAGCAG
    GACGAATCTG AGTGTAAACT GGAGTACGAC AGAAAGCTGG CCACTCAGAA CAGCCTGCAG
    ACGGCAGTGA TGGTGCGTGT GGGCACTGAG GAGGATGAAG AGGATGAAGA AGAGGAGCAG
    AAAGACTCAG TTCAGCAGCA GGAGGACTCT TATGAGCTCA GCAACAGCAA ACACAGCACA
    CTGATCTCAG TGAACAGTGT GTCGACTCCT CCTCAATCTC CAAGCTCCTC CTCCTCTCCT
    CCGCCTCCAC CCTCCCCTCC ACAGCTGACG GACGGGTCAC ACTTCATGTG TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.